FastQCFastQC Report
Thu 26 May 2016
SRR936607_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936607_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026359
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46790.4558833702437451No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.36342059649693725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.22175476611984696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.035.7267651
GTATCAA60500.033.4562531
TTAACGC856.3955446E-427.9495074
GTACATG63550.027.6636371
TCAACGC74800.026.806124
ATCAACG74950.026.7498633
TATCAAC76450.026.3531552
TACATGG65800.026.21352
CAACGCA76500.026.1495915
AACGCAG78150.025.9014976
GTGTAAG3700.025.6833321
ACATGGG65050.025.1972143
GAGTACT40700.022.8776112-13
ACGCAGA88100.022.8121597
CGCAGAG88500.022.7101618
CATGGGG26850.022.6510724
AGTACTT42550.021.65963612-13
GCAGAGT92600.021.1402139
GTACTTT44000.021.11037814-15
CATGGGA36050.021.0881184
Produced by FastQC (version 0.11.5)