##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936607_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026359 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.492770073629206 31.0 31.0 34.0 30.0 34.0 2 31.565180409583782 33.0 31.0 34.0 30.0 34.0 3 31.571204617487645 33.0 31.0 34.0 28.0 34.0 4 35.22515318714017 37.0 35.0 37.0 33.0 37.0 5 35.23167916878987 37.0 35.0 37.0 33.0 37.0 6 35.207695358056974 37.0 35.0 37.0 32.0 37.0 7 35.140573619951695 37.0 35.0 37.0 32.0 37.0 8 35.09964154842506 37.0 35.0 37.0 32.0 37.0 9 36.605407074912385 39.0 37.0 39.0 32.0 39.0 10-11 36.48665525415571 39.0 37.0 39.0 32.0 39.0 12-13 36.3927724119923 39.0 37.0 39.0 32.0 39.0 14-15 37.49378287714143 40.0 37.0 41.0 32.0 41.0 16-17 37.40165916604229 40.0 37.0 41.0 32.0 41.0 18-19 37.37761592191426 40.0 37.0 41.0 32.0 41.0 20-21 37.26060082290894 39.5 37.0 41.0 31.0 41.0 22-23 37.34215318421722 40.0 37.0 41.0 32.0 41.0 24-25 37.37317839079698 40.0 37.0 41.0 32.0 41.0 26-27 37.277272377404 40.0 37.0 41.0 31.0 41.0 28-29 37.130310641792974 39.5 36.0 41.0 31.0 41.0 30-31 36.90786459708542 39.0 36.0 41.0 30.5 41.0 32-33 36.69308838330448 39.0 35.5 41.0 30.0 41.0 34-35 36.496534351040914 39.0 35.0 41.0 30.0 41.0 36-37 36.28887601706615 39.0 35.0 40.0 30.0 41.0 38-39 36.082014188018036 39.0 35.0 40.0 29.5 41.0 40-41 35.839660878893255 38.0 35.0 40.0 28.5 41.0 42-43 35.581843682376245 38.0 35.0 40.0 27.5 41.0 44-45 35.27734545125048 38.0 34.5 40.0 26.5 41.0 46-47 35.029718646204685 38.0 34.0 40.0 25.5 41.0 48-49 34.83151022205681 38.0 34.0 40.0 24.0 41.0 50-51 33.83014422828659 37.5 33.0 39.5 23.5 40.5 52-53 33.91984383631848 37.5 33.0 39.5 23.0 40.5 54-55 34.32772256101423 38.0 34.0 40.0 23.0 41.0 56-57 34.2016755345839 38.0 34.0 40.0 23.0 41.0 58-59 33.964631283985426 38.0 33.5 40.0 22.5 41.0 60-61 33.67224138922151 37.0 33.0 40.0 22.0 41.0 62-63 33.29822508498488 36.5 33.0 40.0 21.0 41.0 64-65 32.92441241320045 36.0 32.0 39.0 20.0 41.0 66-67 32.453726230295636 35.5 32.0 39.0 18.0 41.0 68-69 32.069156113991305 35.0 31.0 39.0 17.0 40.0 70-71 31.66843862625066 35.0 31.0 38.0 13.5 40.0 72-73 31.285886322427146 35.0 31.0 37.0 11.0 39.5 74-75 30.854589378570267 35.0 30.5 37.0 7.5 39.0 76-77 30.386946477791884 34.5 30.0 36.0 7.0 39.0 78-79 29.904759445768974 34.0 29.5 36.0 2.0 37.5 80-81 29.48280036517437 34.0 29.0 35.0 2.0 37.0 82-83 29.088530426488198 34.0 29.0 35.0 2.0 37.0 84-85 28.742953488983872 34.0 29.0 35.0 2.0 36.0 86-87 28.38661228673398 34.0 28.5 35.0 2.0 36.0 88-89 27.980234011685972 34.0 27.0 35.0 2.0 35.5 90-91 27.590385040711872 33.5 26.5 35.0 2.0 35.0 92-93 27.135212922573874 33.0 25.5 35.0 2.0 35.0 94-95 26.628082863793274 33.0 25.0 35.0 2.0 35.0 96-97 26.052132343556202 33.0 24.0 35.0 2.0 35.0 98-99 25.298497406852768 32.5 20.5 35.0 2.0 35.0 100-101 22.49435626325681 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33128119246396537 1101 2 0.24252406570141716 1101 3 0.314012635250176 1101 4 0.3726081906482577 1101 5 0.22570823782404403 1101 6 0.26946161400557855 1101 7 0.32208418297912544 1101 8 0.41889115451033376 1101 9 0.40933408198699084 1101 10-11 0.4575480157490688 1101 12-13 0.46716878472768286 1101 14-15 0.5375052240515998 1101 16-17 0.5356268601043936 1101 18-19 0.4487535033366328 1101 20-21 0.4873737012538726 1101 22-23 0.4414046138367098 1101 24-25 0.41643753387245397 1101 26-27 0.30874393476332074 1101 28-29 0.44134714256757235 1101 30-31 0.3442526512859061 1101 32-33 0.18919081792092385 1101 34-35 0.22066209055229535 1101 36-37 0.210306904356095 1101 38-39 0.2000233018325943 1101 40-41 0.05301199316854621 1101 42-43 0.06941324298784224 1101 44-45 6.216104436091996E-4 1101 46-47 0.35062776044673427 1101 48-49 0.48030078697615153 1101 50-51 0.2893324811661202 1101 52-53 0.31414648615614027 1101 54-55 0.22168623096050766 1101 56-57 0.29763264735503725 1101 58-59 0.20790396960938295 1101 60-61 0.34823586928119 1101 62-63 0.43289007045800787 1101 64-65 0.3283785437858455 1101 66-67 0.5720925774000776 1101 68-69 0.38859925859269495 1101 70-71 0.39257594324466183 1101 72-73 0.16353891154678735 1101 74-75 0.26134053589245454 1101 76-77 0.21564816250510077 1101 78-79 0.0948942576891838 1101 80-81 0.21413375512198485 1101 82-83 0.0029287981190861956 1101 84-85 0.14176259549904557 1101 86-87 0.22215393523062232 1101 88-89 0.2005289448949661 1101 90-91 0.06965381740330656 1101 92-93 0.11333485697740997 1101 94-95 0.1445020296998223 1101 96-97 0.15964074262894812 1101 98-99 0.06267989964943865 1101 100-101 0.3799839592545311 1102 1 0.19877002987041337 1102 2 0.22517835335666447 1102 3 0.18513446864447403 1102 4 0.13081973703761918 1102 5 0.24838876991250913 1102 6 0.24510770898487522 1102 7 0.3089015809480884 1102 8 0.3408789961379526 1102 9 0.2596769434430968 1102 10-11 0.38502454381958273 1102 12-13 0.3991181887119595 1102 14-15 0.3925755672614315 1102 16-17 0.21808605639765943 1102 18-19 0.14013261346454442 1102 20-21 0.09918050274259826 1102 22-23 0.06858473077540594 1102 24-25 0.08090797114419956 1102 26-27 -0.08827163190159837 1102 28-29 -0.20622031201272506 1102 30-31 0.05522189536494437 1102 32-33 -0.035247858941581 1102 34-35 0.17870322231445357 1102 36-37 -0.10448084669338442 1102 38-39 -0.31659923702600423 1102 40-41 -0.17705791456950237 1102 42-43 -0.21460494231411786 1102 44-45 -0.07249061934614787 1102 46-47 0.11972377907331833 1102 48-49 0.4218935685957561 1102 50-51 0.29709607224069856 1102 52-53 0.3119125158727414 1102 54-55 0.4149523855123505 1102 56-57 0.36098508338423585 1102 58-59 0.3653749236409851 1102 60-61 0.13688370034135744 1102 62-63 0.11040492004605795 1102 64-65 0.04681409686404692 1102 66-67 0.15321661718478197 1102 68-69 0.085562278328279 1102 70-71 0.13067769268000262 1102 72-73 -0.17306858901932287 1102 74-75 -0.28112260867837335 1102 76-77 0.04169953656375469 1102 78-79 -0.13070712885299685 1102 80-81 0.2265943449249761 1102 82-83 0.143280775451327 1102 84-85 -0.14306992205046853 1102 86-87 -0.0867413018692531 1102 88-89 0.0382291893397948 1102 90-91 -0.04375348807144519 1102 92-93 -0.1014052824896936 1102 94-95 -0.17650832291619878 1102 96-97 -0.245143640772028 1102 98-99 0.036286535078261295 1102 100-101 0.31809964378715705 1103 1 0.22249594908308978 1103 2 0.08177814176299947 1103 3 0.2623781641265559 1103 4 0.29517391500128554 1103 5 0.38116695427707725 1103 6 0.33260181558308943 1103 7 0.42147471917031964 1103 8 0.36471361938367863 1103 9 0.18179366376657669 1103 10-11 0.44226006121917294 1103 12-13 -0.06777795722447166 1103 14-15 0.37627154230500537 1103 16-17 0.46024135401287225 1103 18-19 0.4844488461366865 1103 20-21 0.4208951445438487 1103 22-23 0.051673985634536734 1103 24-25 0.28557712536361635 1103 26-27 0.11545426203107212 1103 28-29 0.2330375175216588 1103 30-31 0.06406831805425384 1103 32-33 0.08218852075907535 1103 34-35 0.28232407781518987 1103 36-37 0.34876662309384443 1103 38-39 0.26608756011078327 1103 40-41 0.07708705869057297 1103 42-43 0.30925717293997224 1103 44-45 0.3542251663754499 1103 46-47 0.45383289264248816 1103 48-49 0.34760731801273437 1103 50-51 0.2941487393446991 1103 52-53 0.36043211862269686 1103 54-55 0.38232632359402174 1103 56-57 0.3326332387652755 1103 58-59 0.1765000862230366 1103 60-61 0.36471629360246993 1103 62-63 0.37558335266029985 1103 64-65 0.32913648269005336 1103 66-67 0.5373643459656918 1103 68-69 0.43226494372588675 1103 70-71 0.30241047288676626 1103 72-73 0.06702480796906585 1103 74-75 0.0857418108484076 1103 76-77 0.31811714459470863 1103 78-79 0.31926689593910496 1103 80-81 0.46843206244363955 1103 82-83 0.21884240242206232 1103 84-85 0.28731083278528047 1103 86-87 0.2120149849526598 1103 88-89 0.26141089318703337 1103 90-91 0.1315981252354419 1103 92-93 0.18776103341686934 1103 94-95 0.34627812801088 1103 96-97 0.35074437637792855 1103 98-99 0.4502210649143592 1103 100-101 0.24626081394815102 1104 1 0.3796484769027728 1104 2 0.3842534687161283 1104 3 0.44166262195691175 1104 4 0.5839003967577767 1104 5 0.45486154111572574 1104 6 0.4845647798213619 1104 7 0.5911361209349266 1104 8 0.44115922072761293 1104 9 0.4591669907211724 1104 10-11 0.5694941157749867 1104 12-13 0.5660938813983094 1104 14-15 0.3384833726197982 1104 16-17 0.4054516582655552 1104 18-19 0.44016117573679026 1104 20-21 0.12240750652607346 1104 22-23 0.2911794697141872 1104 24-25 0.3508016279281776 1104 26-27 0.13217171196150446 1104 28-29 0.3090265090678699 1104 30-31 0.3059559389285553 1104 32-33 0.2666674874032964 1104 34-35 0.2547919539514041 1104 36-37 0.21848209273341723 1104 38-39 0.40139974347854235 1104 40-41 0.13632709734397253 1104 42-43 0.4494730121898769 1104 44-45 0.5326714988811645 1104 46-47 0.3134668648510086 1104 48-49 0.5518935890949948 1104 50-51 0.5514121868970321 1104 52-53 0.6221387659675415 1104 54-55 0.3644410661745283 1104 56-57 0.6118542510946483 1104 58-59 0.536812104749707 1104 60-61 0.4723749201758807 1104 62-63 0.5593069808344993 1104 64-65 0.6006674753777048 1104 66-67 0.5454690325075475 1104 68-69 0.7057113897887923 1104 70-71 0.6831774381477906 1104 72-73 0.5534131471841732 1104 74-75 0.6076722684252758 1104 76-77 0.5311507992826776 1104 78-79 0.7800909474984685 1104 80-81 0.8161324560841443 1104 82-83 0.6305352031160041 1104 84-85 0.4791854861611142 1104 86-87 0.44880608587862625 1104 88-89 0.48121585321856486 1104 90-91 0.5772765781615945 1104 92-93 0.37272023708059265 1104 94-95 0.28434513554448415 1104 96-97 0.25720420533882304 1104 98-99 0.3088194688131054 1104 100-101 0.32419987549688045 1105 1 0.3773259088213763 1105 2 0.23478504648575793 1105 3 0.3528587847171245 1105 4 0.34516809014841954 1105 5 0.329313783532875 1105 6 0.46138063724426814 1105 7 0.4874455155888242 1105 8 0.5882371844249761 1105 9 0.6567447308713596 1105 10-11 0.7105852135948538 1105 12-13 0.4368666399972838 1105 14-15 0.5139384284708584 1105 16-17 0.5091748688943412 1105 18-19 0.5262493949121847 1105 20-21 0.4602670188493079 1105 22-23 0.14864435985447955 1105 24-25 0.22292504919602152 1105 26-27 0.1327697732424653 1105 28-29 0.23627072397510318 1105 30-31 0.22849338028929367 1105 32-33 0.03224577782346216 1105 34-35 0.2432876489374678 1105 36-37 0.25539216602383874 1105 38-39 0.48807642100916837 1105 40-41 0.24780254891597053 1105 42-43 0.36948801061991077 1105 44-45 0.42308112396060693 1105 46-47 0.6713748295176032 1105 48-49 0.624521856267144 1105 50-51 0.7183740132573746 1105 52-53 0.7891666961886656 1105 54-55 0.8681116170385437 1105 56-57 0.6596797243960637 1105 58-59 0.8560548939574488 1105 60-61 0.9041334034674549 1105 62-63 0.7511341317446991 1105 64-65 0.9261979476942557 1105 66-67 0.9663172522367844 1105 68-69 0.9434003273523537 1105 70-71 0.9645737571453701 1105 72-73 0.7317915122838379 1105 74-75 1.0018837587948788 1105 76-77 0.8355644379384266 1105 78-79 0.8185933198677695 1105 80-81 1.025995241441784 1105 82-83 0.9211221920002828 1105 84-85 0.6634484348131551 1105 86-87 0.6162252794919212 1105 88-89 0.42277914699528907 1105 90-91 0.33838102181497476 1105 92-93 0.2506494085803581 1105 94-95 0.5089881635300912 1105 96-97 0.500472219422516 1105 98-99 0.5756642288091953 1105 100-101 0.5805743199229632 1106 1 0.36690659185185126 1106 2 0.2552548698584154 1106 3 0.33559387495017035 1106 4 0.399341954479695 1106 5 0.3804411944715369 1106 6 0.34408492816829295 1106 7 0.4868178122799023 1106 8 0.5926433826743249 1106 9 0.6095325147048456 1106 10-11 0.5941786113835477 1106 12-13 0.5336046472373468 1106 14-15 0.5586660554510559 1106 16-17 0.5630786813131508 1106 18-19 0.5819001753845257 1106 20-21 0.4734468354818304 1106 22-23 0.7302616918308544 1106 24-25 0.6284158071714856 1106 26-27 0.5164315209470232 1106 28-29 0.5086788951656942 1106 30-31 0.7024623845788796 1106 32-33 0.5661339450860297 1106 34-35 0.5206574660609959 1106 36-37 0.46202679471361563 1106 38-39 0.5064307447411451 1106 40-41 0.5225008607567432 1106 42-43 0.5323912472255543 1106 44-45 0.6440321307409178 1106 46-47 0.544966542271645 1106 48-49 0.661441691022155 1106 50-51 0.24389559286019846 1106 52-53 0.3590208681559375 1106 54-55 0.40106520381895194 1106 56-57 0.6243319377058256 1106 58-59 0.6131649590823613 1106 60-61 0.5087031951480725 1106 62-63 0.6617885166289383 1106 64-65 0.39588350460379473 1106 66-67 0.5492496923901555 1106 68-69 0.5511362545231471 1106 70-71 0.39773897758361443 1106 72-73 0.1498218295167213 1106 74-75 0.3331668736316651 1106 76-77 0.28639741417832454 1106 78-79 0.2582888865527373 1106 80-81 0.5628124118333808 1106 82-83 0.3472108248234207 1106 84-85 0.3633883984788966 1106 86-87 0.2758644588912027 1106 88-89 0.18374498431244746 1106 90-91 0.26496037913605974 1106 92-93 0.22787047396862903 1106 94-95 0.1503129132299108 1106 96-97 0.09729587329184852 1106 98-99 0.1628114874475095 1106 100-101 0.30598453100982326 1107 1 0.24162658840565499 1107 2 0.12494018906234317 1107 3 0.3796836483627679 1107 4 0.3029292294000001 1107 5 0.38849667823134126 1107 6 0.4007102404798317 1107 7 0.5657787841072519 1107 8 0.568100003678893 1107 9 0.5925761521650017 1107 10-11 0.6697256950399577 1107 12-13 0.5207971819146948 1107 14-15 0.24358948527788016 1107 16-17 0.3821614546395935 1107 18-19 0.34455417727993165 1107 20-21 0.3288789023380332 1107 22-23 0.38910441604900114 1107 24-25 0.38577128231579394 1107 26-27 0.30732279345499336 1107 28-29 0.43269026761335283 1107 30-31 0.4211531530806454 1107 32-33 0.06431123034492714 1107 34-35 0.32085346847333085 1107 36-37 0.3573835837202495 1107 38-39 0.3424725729482603 1107 40-41 0.4073198277594159 1107 42-43 0.28861055454010653 1107 44-45 0.30754875863787845 1107 46-47 0.3705567033813182 1107 48-49 0.473957845067126 1107 50-51 0.4356441066880663 1107 52-53 0.6024380948295018 1107 54-55 0.768908961895967 1107 56-57 0.5361362368356666 1107 58-59 0.6694429594269806 1107 60-61 0.8884417150067776 1107 62-63 0.8183050583709885 1107 64-65 0.8661573695128979 1107 66-67 0.8516668759862611 1107 68-69 0.8987120708408902 1107 70-71 0.8843984287768656 1107 72-73 0.6685605216852402 1107 74-75 0.7438376972725713 1107 76-77 0.8180458806209892 1107 78-79 0.6871599341963979 1107 80-81 0.4747620973679716 1107 82-83 0.7235408411928503 1107 84-85 0.4388708659554226 1107 86-87 0.5824924635435664 1107 88-89 0.38138703221457604 1107 90-91 0.49237971660483026 1107 92-93 0.382658532898585 1107 94-95 0.6684027512586859 1107 96-97 0.7448226890065008 1107 98-99 0.7922592344967043 1107 100-101 0.46711036025079977 1108 1 0.13073085759038605 1108 2 0.21871429466149195 1108 3 0.15594711424918728 1108 4 0.1549092134404333 1108 5 0.06291814385176764 1108 6 0.16092103978824923 1108 7 0.0070641944011740065 1108 8 0.16165632765814308 1108 9 0.06850546457356899 1108 10-11 0.15643436640583275 1108 12-13 0.06816302178702216 1108 14-15 0.16729858888209037 1108 16-17 0.19309791560515066 1108 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14 5974.0 15 7541.0 16 9423.0 17 12389.0 18 9687.0 19 7030.0 20 7064.0 21 7460.0 22 8349.0 23 9381.0 24 10682.0 25 12149.0 26 14107.0 27 16379.0 28 19540.0 29 23514.0 30 28484.0 31 34679.0 32 43804.0 33 56109.0 34 75497.0 35 103121.0 36 144471.0 37 182620.0 38 133280.0 39 17581.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.56156010137969 18.17078054764914 14.465802866967895 22.80185648400327 2 15.734058579689075 19.798757778501034 39.8430755018875 24.624108139922395 3 19.080462906300063 24.82313596021095 29.570595549457618 26.52580558403137 4 12.65131626390375 16.144331986567423 36.65863674546514 34.54571500406369 5 13.903392029223516 37.18366937193416 33.423324818254564 15.489613780587765 6 32.041916459521374 35.251352536920606 17.624116586245552 15.082614417312474 7 29.27671515848347 32.6774108682731 20.09548957896891 17.950384394274526 8 27.52009605563742 33.04592660164199 19.618506303651206 19.815471039069383 9 27.49744631411506 16.098065436738768 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22.846588096824856 34-35 22.657276839780234 25.664606633741215 29.061663608932157 22.616452917546397 36-37 22.844775930355482 25.39326061645337 28.93492017869061 22.82704327450054 38-39 22.403905456083105 25.531076358272298 29.145260089305985 22.91975809633861 40-41 22.346719254212978 25.52511224219371 29.346705613668604 22.781462889924704 42-43 22.443316482102084 25.63077632097528 28.77672922424414 23.149177972678498 44-45 22.34261102810395 25.86645595242561 28.65526932563486 23.135663693835586 46-47 22.789999332587325 26.054524205401364 28.12140869867682 23.03406776333449 48-49 22.84151466774752 26.27815741308647 27.632616732782804 23.24771118638321 50-51 22.711536925058606 26.322586868448695 27.680445318330893 23.285430888161805 52-53 23.019559717573053 26.33344327321392 27.47577871781518 23.171218291397846 54-55 22.59342025583726 26.199179298004076 27.559724509934313 23.647675936224353 56-57 22.757744803305307 26.57471943036948 27.253825329480847 23.41371043684437 58-59 23.03104119419564 26.00878400268928 27.336079450646622 23.624095352468462 60-61 22.852507733904954 26.07653521058144 27.951867098628604 23.119089956885002 62-63 22.632163284872124 26.265950279314097 27.88016019821631 23.221726237597476 64-65 22.527057099541288 26.23895598763772 27.83578279491946 23.398204117901532 66-67 22.529856494905236 26.469096438756363 27.397716138318 23.6033309280204 68-69 22.427378652511166 26.31138528838975 27.77126882911053 23.489967229988558 70-71 22.536993413590725 26.28880476143824 27.647516704855462 23.526685120115577 72-73 22.659805699033758 26.339430798247694 27.40380328622874 23.59696021648981 74-75 22.40358042481483 26.707770338593612 27.254151586196613 23.634497650394948 76-77 22.532742537449803 26.493472446174664 27.39121515174108 23.582569864634454 78-79 22.586530624978067 26.339928092621694 27.34766476499468 23.725876517405563 80-81 22.446724900197555 26.85436546708713 27.262204956285846 23.43670467642946 82-83 22.625180702573555 26.733258144166292 27.16363001860883 23.47793113465132 84-85 22.443163546711624 26.464007639910736 26.94983385143102 24.142994961946616 86-87 22.53624547768994 26.433337799812406 27.12991363453644 23.900503087961216 88-89 22.49243898196319 26.36491785082417 27.084645883185864 24.057997284026776 90-91 22.320487384809724 26.32656751128571 27.391856743720915 23.961088360183652 92-93 22.421242567191904 26.211578783745786 27.387281766712036 23.97989688235028 94-95 22.142961895764522 26.20518219941153 27.374516979471842 24.277338925352105 96-97 22.25589657059245 26.252413711783202 27.25965569981928 24.232034017805066 98-99 22.138537920654155 26.31610244752211 26.970607703353338 24.57475192847039 100-101 22.15035290607599 26.195780727301443 27.09679054861075 24.55707581801181 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 207.0 1 165.0 2 163.0 3 218.5 4 201.5 5 199.5 6 342.5 7 653.0 8 1039.5 9 1314.0 10 1266.5 11 1110.0 12 1142.5 13 1232.5 14 1299.5 15 1373.0 16 1430.0 17 1500.0 18 1623.5 19 1722.0 20 1856.0 21 2078.5 22 2354.0 23 2762.5 24 3279.0 25 3901.0 26 4653.5 27 5598.5 28 6755.5 29 8318.5 30 10197.0 31 12174.5 32 14674.0 33 16827.5 34 18520.5 35 19775.5 36 21738.0 37 23855.0 38 24812.5 39 25504.5 40 26237.0 41 28422.5 42 30230.0 43 31853.5 44 34020.0 45 35811.0 46 36321.5 47 37095.0 48 40688.0 49 40921.5 50 37893.5 51 35907.5 52 35052.0 53 35891.0 54 36668.5 55 35122.5 56 31945.5 57 29033.5 58 27121.5 59 24550.0 60 21120.0 61 18171.0 62 15525.5 63 12493.5 64 9502.5 65 7579.0 66 5680.5 67 4387.5 68 3348.0 69 2182.0 70 1624.0 71 1286.0 72 1068.0 73 950.5 74 776.0 75 625.0 76 489.0 77 343.5 78 238.0 79 147.5 80 89.5 81 63.0 82 46.5 83 21.5 84 10.0 85 13.5 86 13.5 87 8.5 88 4.5 89 3.5 90 5.5 91 7.0 92 5.0 93 3.5 94 2.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011496951846283805 2 0.012958428775896154 3 0.014322473910201012 4 0.019194063675575506 5 0.032737083223316595 6 0.049592783811512345 7 0.006722793876216802 8 0.027962925253249594 9 0.03809583196522854 10-11 0.04223668326579686 12-13 0.030057708852360625 14-15 0.02849880012744079 16-17 0.029132106796939474 18-19 0.02377335805502753 20-21 0.052808033056659515 22-23 0.01232512210639747 24-25 0.003117817449839676 26-27 0.001705056417881073 28-29 4.871589765374494E-5 30-31 0.0013640451343048582 32-33 0.0010717497483823887 34-35 0.0 36-37 3.897271812299595E-4 38-39 0.0 40-41 6.820225671524291E-4 42-43 0.0023870789850335018 44-45 8.281702601136639E-4 46-47 7.307384648061741E-4 48-49 8.281702601136639E-4 50-51 0.003604976426377125 52-53 0.00394598770995334 54-55 0.0041408513005683194 56-57 9.256020554211539E-4 58-59 0.005992055411410627 60-61 0.0033126810404546557 62-63 5.358748741911944E-4 64-65 0.0010717497483823887 66-67 0.015442939556237144 68-69 0.04101878582445324 70-71 0.05207729459185333 72-73 0.016319825714004553 74-75 0.032688367325662854 76-77 0.027816777560288358 78-79 0.057971918207956476 80-81 0.08116068549113907 82-83 0.04891076124435992 84-85 0.01646597340696579 86-87 0.01914534777792176 88-89 0.02021709752630415 90-91 0.038339411453497264 92-93 0.024309232929218723 94-95 0.013104576468857387 96-97 0.01856075700607682 98-99 0.05319776023788947 100-101 0.0713200741650826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.31241196811808 #Duplication Level Percentage of deduplicated Percentage of total 1 78.08624097199413 42.410520887126864 2 12.194913471801963 13.246703287921225 3 3.9239622635588867 6.393595650172782 4 1.835871934841378 3.9884253138324373 5 0.9727673328628119 2.6416670065786243 6 0.5809007368340813 1.8930072078909579 7 0.40318215446647687 1.5328456690103698 8 0.2877356330198048 1.25020929907831 9 0.22444692799573956 1.0971228616454827 >10 1.3063603911500092 13.442174976465243 >50 0.12007333008451318 4.487589729765412 >100 0.060656449297870306 5.725487151244837 >500 0.002346826699983962 0.8430128583613493 >1k 5.415753923039912E-4 1.047638100906143 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4679 0.4558833702437451 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3730 0.36342059649693725 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2276 0.22175476611984696 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.743179530748988E-5 0.0 0.0 0.0 0.0 6 9.743179530748988E-5 0.0 0.0 0.0 0.0 7 9.743179530748988E-5 0.0 0.0 0.0 0.0 8 9.743179530748988E-5 0.0 0.0 0.0 0.0 9 9.743179530748988E-5 0.0 0.0 0.0 0.0 10-11 9.743179530748988E-5 0.0 0.0 0.0 0.0 12-13 9.743179530748988E-5 0.0 0.0 0.0 0.0 14-15 9.743179530748988E-5 0.0 0.0 9.743179530748988E-5 4.871589765374494E-5 16-17 9.743179530748988E-5 0.0 0.0 9.743179530748988E-5 9.743179530748988E-5 18-19 9.743179530748988E-5 0.0 0.0 9.743179530748988E-5 9.743179530748988E-5 20-21 9.743179530748988E-5 0.0 0.0 9.743179530748988E-5 9.743179530748988E-5 22-23 9.743179530748988E-5 0.0 0.0 1.4614769296123482E-4 9.743179530748988E-5 24-25 9.743179530748988E-5 0.0 0.0 3.410112835762146E-4 9.743179530748988E-5 26-27 9.743179530748988E-5 0.0 0.0 8.281702601136639E-4 9.743179530748988E-5 28-29 9.743179530748988E-5 0.0 0.0 0.0020947835991110323 9.743179530748988E-5 30-31 9.743179530748988E-5 0.0 0.0 0.0035075446310696354 9.743179530748988E-5 32-33 9.743179530748988E-5 0.0 0.0 0.005310032844258198 9.743179530748988E-5 34-35 9.743179530748988E-5 0.0 0.0 0.00779454362459919 2.9229538592246963E-4 36-37 9.743179530748988E-5 0.0 0.0 0.010327770302593926 2.9229538592246963E-4 38-39 9.743179530748988E-5 0.0 0.0 0.013591735445394836 2.9229538592246963E-4 40-41 9.743179530748988E-5 0.0 0.0 0.01807359802953937 2.9229538592246963E-4 42-43 9.743179530748988E-5 0.0 0.0 0.026014289347099796 2.9229538592246963E-4 44-45 9.743179530748988E-5 0.0 0.0 0.041505944800990686 3.410112835762146E-4 46-47 9.743179530748988E-5 0.0 0.0 0.057582191026726515 4.384430788837044E-4 48-49 9.743179530748988E-5 0.0 0.0 0.08612970705182105 5.845907718449393E-4 50-51 9.743179530748988E-5 0.0 0.0 0.11872064258217642 5.845907718449393E-4 52-53 9.743179530748988E-5 0.0 0.0 0.14980138528526568 5.845907718449393E-4 54-55 9.743179530748988E-5 0.0 0.0 0.20670155374483978 5.845907718449393E-4 56-57 9.743179530748988E-5 0.0 0.0 0.26413759707860507 5.845907718449393E-4 58-59 9.743179530748988E-5 0.0 0.0 0.3290758886510471 5.845907718449393E-4 60-61 9.743179530748988E-5 0.0 0.0 0.4007369740997059 5.845907718449393E-4 62-63 9.743179530748988E-5 0.0 0.0 0.4988020760766944 6.820225671524291E-4 64-65 9.743179530748988E-5 0.0 0.0 0.5677837871543973 6.820225671524291E-4 66-67 9.743179530748988E-5 0.0 0.0 0.6791970450885119 6.820225671524291E-4 68-69 9.743179530748988E-5 0.0 0.0 0.8324572591071935 6.820225671524291E-4 70-71 9.743179530748988E-5 0.0 0.0 0.9212176246323167 6.820225671524291E-4 72-73 9.743179530748988E-5 0.0 0.0 1.0658551247662855 8.768861577674088E-4 74-75 9.743179530748988E-5 0.0 0.0 1.2539472056073948 8.768861577674088E-4 76-77 9.743179530748988E-5 0.0 0.0 1.3842622318311624 9.256020554211538E-4 78-79 9.743179530748988E-5 0.0 0.0 1.5053212375007186 9.743179530748988E-4 80-81 9.743179530748988E-5 0.0 0.0 1.6993566578555845 9.743179530748988E-4 82-83 9.743179530748988E-5 0.0 0.0 1.9394773173908935 9.743179530748988E-4 84-85 9.743179530748988E-5 0.0 0.0 2.2053686867850333 9.743179530748988E-4 86-87 9.743179530748988E-5 0.0 0.0 2.6236433840400872 9.743179530748988E-4 88-89 9.743179530748988E-5 0.0 0.0 3.0758730619598014 9.743179530748988E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3910 0.0 35.726765 1 GTATCAA 6050 0.0 33.456253 1 TTAACGC 85 6.3955446E-4 27.949507 4 GTACATG 6355 0.0 27.663637 1 TCAACGC 7480 0.0 26.80612 4 ATCAACG 7495 0.0 26.749863 3 TATCAAC 7645 0.0 26.353155 2 TACATGG 6580 0.0 26.2135 2 CAACGCA 7650 0.0 26.149591 5 AACGCAG 7815 0.0 25.901497 6 GTGTAAG 370 0.0 25.683332 1 ACATGGG 6505 0.0 25.197214 3 GAGTACT 4070 0.0 22.87761 12-13 ACGCAGA 8810 0.0 22.812159 7 CGCAGAG 8850 0.0 22.710161 8 CATGGGG 2685 0.0 22.651072 4 AGTACTT 4255 0.0 21.659636 12-13 GCAGAGT 9260 0.0 21.140213 9 GTACTTT 4400 0.0 21.110378 14-15 CATGGGA 3605 0.0 21.088118 4 >>END_MODULE