FastQCFastQC Report
Thu 26 May 2016
SRR936607_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936607_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026359
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52490.5114194935690143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46480.4528629845892129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27770.2705680955688994No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13810.13455330931964352No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12740.1241281072217421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.030.178411
GTATCAA72700.029.3467731
TCGTAGA856.393365E-427.951142
GTACATG63700.026.4065861
TACATGG66300.025.3710352
ACATGGG64900.024.6712593
GTCTACG1000.001650097323.7584691
GAACCGA1201.711594E-423.7480536
ATCAACG91150.023.352183
GTAAGGT4500.023.2248444
TCAACGC91650.023.2213864
TAAGGTG5350.023.0867165
TATCAAC93100.022.9673712
CAACGCA94050.022.6288175
AGGTAAG4450.022.4237232
AACGCAG95200.022.4016
GAGTACT50300.022.0966812-13
CATGGGG23500.021.63014
AGTACTT52100.021.42442912-13
CATGGGA40800.021.4239854
Produced by FastQC (version 0.11.5)