##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936607_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1026359 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965183722264822 31.0 31.0 34.0 30.0 34.0 2 32.085183644319386 33.0 31.0 34.0 30.0 34.0 3 32.18409055700783 33.0 31.0 34.0 30.0 34.0 4 35.92280478857788 37.0 35.0 37.0 35.0 37.0 5 35.779468002911266 37.0 35.0 37.0 35.0 37.0 6 35.83617038482636 37.0 35.0 37.0 35.0 37.0 7 35.764365100320646 37.0 35.0 37.0 33.0 37.0 8 35.736227772153796 37.0 35.0 37.0 33.0 37.0 9 37.347662952241855 39.0 37.0 39.0 34.0 39.0 10-11 37.243952652044754 39.0 37.0 39.0 33.5 39.0 12-13 37.12500207042565 39.0 37.0 39.0 33.0 39.0 14-15 38.22741263047335 40.0 38.0 41.0 33.0 41.0 16-17 38.18753866824376 40.0 37.5 41.0 33.0 41.0 18-19 38.32307896164987 40.0 38.0 41.0 33.5 41.0 20-21 38.33591754931754 40.0 38.0 41.0 34.0 41.0 22-23 38.240376905156964 40.0 38.0 41.0 33.5 41.0 24-25 38.10817170210424 40.0 37.0 41.0 33.0 41.0 26-27 37.9022320649987 40.0 37.0 41.0 33.0 41.0 28-29 37.914248328313974 40.0 37.0 41.0 33.0 41.0 30-31 37.78523304223961 40.0 37.0 41.0 33.0 41.0 32-33 37.57209855420959 40.0 36.5 41.0 33.0 41.0 34-35 37.375537214561376 39.5 36.0 41.0 32.0 41.0 36-37 37.18106188965069 39.0 36.0 41.0 31.5 41.0 38-39 36.95980012841511 39.0 35.5 41.0 31.0 41.0 40-41 36.70817715828477 39.0 35.0 41.0 31.0 41.0 42-43 36.40467711590194 39.0 35.0 40.0 30.0 41.0 44-45 36.06057139850676 39.0 35.0 40.0 30.0 41.0 46-47 35.588703367924865 38.0 35.0 40.0 28.5 41.0 48-49 35.123664819035056 38.0 34.5 40.0 26.5 41.0 50-51 34.78004869641129 38.0 34.0 40.0 26.0 41.0 52-53 34.49105381255487 38.0 34.0 40.0 24.5 41.0 54-55 34.121198333136846 38.0 33.0 40.0 24.0 41.0 56-57 33.773833035029654 37.0 33.0 40.0 23.0 41.0 58-59 33.38364987299765 37.0 32.5 39.5 22.0 41.0 60-61 32.99154779175707 36.0 32.0 39.0 21.5 40.0 62-63 32.65947733687725 36.0 32.0 39.0 20.0 40.0 64-65 32.33985330668899 35.0 31.0 39.0 19.5 40.0 66-67 31.89283915277208 35.0 31.0 38.0 18.0 40.0 68-69 31.43174756591017 35.0 30.5 38.0 15.5 40.0 70-71 30.933475031640974 34.5 30.0 37.0 12.0 39.0 72-73 30.392554164770807 34.0 29.5 36.5 8.0 39.0 74-75 29.857704273066247 34.0 29.0 36.0 7.0 38.5 76-77 28.48453221533596 32.5 27.0 34.5 7.0 36.5 78-79 29.274006463625298 33.5 29.0 35.0 3.5 37.0 80-81 29.153614378594625 34.0 29.0 35.0 2.0 37.0 82-83 28.762686837646477 34.0 29.0 35.0 2.0 36.0 84-85 28.333730205512886 33.0 27.5 35.0 2.0 36.0 86-87 27.90303831310487 33.0 27.0 35.0 2.0 35.0 88-89 27.430478029617316 33.0 26.0 35.0 2.0 35.0 90-91 26.879991309083856 32.0 25.5 34.0 2.0 35.0 92-93 26.300031470469882 32.0 24.5 34.0 2.0 35.0 94-95 25.54812692245111 32.0 23.5 34.0 2.0 35.0 96-97 24.7451559347168 31.0 19.0 34.0 2.0 35.0 98-99 23.565360171246123 31.0 2.0 34.0 2.0 35.0 100-101 19.928411501238845 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29814578052704377 1101 2 0.38034928342110064 1101 3 0.2255559504828355 1101 4 0.15264902423221827 1101 5 0.17605470328128092 1101 6 0.15698358716364424 1101 7 0.24165901922523858 1101 8 0.1106250854380022 1101 9 0.27142630297245773 1101 10-11 0.20972683726075303 1101 12-13 0.27816786607458255 1101 14-15 0.28373744945176327 1101 16-17 0.3123859715988502 1101 18-19 0.2625399357648419 1101 20-21 0.17346918582585147 1101 22-23 0.19625877641312428 1101 24-25 0.2558440915516442 1101 26-27 0.09785188529030364 1101 28-29 0.14462891853745674 1101 30-31 0.28353590469265555 1101 32-33 0.16685073954012353 1101 34-35 0.16923891773485877 1101 36-37 0.22378159677106169 1101 38-39 0.20103650569517129 1101 40-41 0.38150254949623275 1101 42-43 0.47163292402573376 1101 44-45 0.595034554370443 1101 46-47 0.3615370890289711 1101 48-49 0.40447670134254565 1101 50-51 0.4260280636749698 1101 52-53 0.24039450807114093 1101 54-55 0.3851037857889352 1101 56-57 0.22027627031470587 1101 58-59 0.044885744396033544 1101 60-61 0.202994559179011 1101 62-63 0.16914381844256354 1101 64-65 0.2558060762074632 1101 66-67 0.22448483810000397 1101 68-69 0.0931101513804613 1101 70-71 0.21694954300778235 1101 72-73 0.3350225278797829 1101 74-75 0.2037292044385275 1101 76-77 0.25156434733064614 1101 78-79 0.27271734460112285 1101 80-81 0.37941785099278036 1101 82-83 0.20635996904841392 1101 84-85 0.12097808508516295 1101 86-87 0.08504842257648093 1101 88-89 0.09820946426403054 1101 90-91 0.22595078746974906 1101 92-93 0.34175967152158293 1101 94-95 0.11454545210157718 1101 96-97 -0.05575606274542366 1101 98-99 -0.6143516669112898 1101 100-101 -0.4790983491764358 1102 1 0.19521708758110634 1102 2 0.3311589706362277 1102 3 0.21012863653089653 1102 4 0.2302106144590681 1102 5 0.08079379761608152 1102 6 0.1024816797972008 1102 7 0.17057957175184413 1102 8 0.1429051576682525 1102 9 0.21690523484725333 1102 10-11 0.2048478113240506 1102 12-13 0.24255614433524642 1102 14-15 0.3522304848062703 1102 16-17 0.3817827560751539 1102 18-19 0.2955838255149956 1102 20-21 0.27687457522473125 1102 22-23 0.2869312609829038 1102 24-25 0.13148467055493995 1102 26-27 0.23473750717304398 1102 28-29 0.28828932021663434 1102 30-31 0.4030540406411376 1102 32-33 0.38784889894602514 1102 34-35 0.32817247635226465 1102 36-37 0.3532702948265438 1102 38-39 0.5035732740299821 1102 40-41 0.582860350537878 1102 42-43 0.6603425920801058 1102 44-45 0.6580145168618117 1102 46-47 0.611349545844682 1102 48-49 0.9355940638931415 1102 50-51 0.8516175473215597 1102 52-53 0.9138742760517644 1102 54-55 0.9126572472090402 1102 56-57 0.974416445780804 1102 58-59 0.9600090990384587 1102 60-61 0.8204462363260276 1102 62-63 0.7878020427627916 1102 64-65 0.7916250825188982 1102 66-67 0.7252762354999476 1102 68-69 0.7136771937141226 1102 70-71 0.3902975081932638 1102 72-73 0.24873205077689065 1102 74-75 0.46724421660270465 1102 76-77 0.41502507067832184 1102 78-79 0.7368919513700938 1102 80-81 0.8253813178127345 1102 82-83 0.8090172179118333 1102 84-85 0.4289315418602335 1102 86-87 0.5370794630056785 1102 88-89 0.3329577906007799 1102 90-91 0.488174480618909 1102 92-93 0.5416026113626096 1102 94-95 0.5232932094218583 1102 96-97 0.6113292374688264 1102 98-99 0.5505687578769276 1102 100-101 0.30316285260958153 1103 1 0.16970766382623736 1103 2 0.23856272648696475 1103 3 0.22032975163203616 1103 4 0.11697640403586718 1103 5 0.0716241068284944 1103 6 0.13628547407355285 1103 7 0.18808534507360264 1103 8 0.17344530295245875 1103 9 -0.019298516958684786 1103 10-11 0.12403647475741053 1103 12-13 0.1408256014091691 1103 14-15 0.2510365100432992 1103 16-17 0.199588476688092 1103 18-19 0.20782017083484305 1103 20-21 0.11014502453894437 1103 22-23 0.15469975678453096 1103 24-25 0.09883743663018407 1103 26-27 0.07801536352951643 1103 28-29 0.3000549517933848 1103 30-31 0.32322420037287003 1103 32-33 0.1761212505808203 1103 34-35 0.15179449473335893 1103 36-37 0.2782212200116163 1103 38-39 0.10697893346530662 1103 40-41 0.17093716131323333 1103 42-43 0.3944187152392473 1103 44-45 0.21189348124101315 1103 46-47 0.26839495758648724 1103 48-49 0.30361946777286164 1103 50-51 0.43829826967249375 1103 52-53 0.4560015128584425 1103 54-55 0.4233652264642629 1103 56-57 0.33089457415015744 1103 58-59 0.16761768615146622 1103 60-61 -0.014646585542188006 1103 62-63 0.29226048811815986 1103 64-65 0.40890662217248774 1103 66-67 0.33486190881399125 1103 68-69 0.4509357538407279 1103 70-71 0.2851487922280569 1103 72-73 0.4801673611332262 1103 74-75 0.34799968435237005 1103 76-77 0.1346590414470512 1103 78-79 0.12506773337553412 1103 80-81 0.429821877562663 1103 82-83 0.3671030915699376 1103 84-85 0.6054292875155483 1103 86-87 0.41661043038309487 1103 88-89 0.30833285111479825 1103 90-91 0.4859798599517404 1103 92-93 0.38098355453441357 1103 94-95 0.32377612076866313 1103 96-97 0.3404523751222648 1103 98-99 0.3739461921836629 1103 100-101 0.41152655355214307 1104 1 0.13404667672853066 1104 2 0.2685088564213025 1104 3 0.12434205683566546 1104 4 0.081356077738441 1104 5 0.026404534843862848 1104 6 -0.02235868238334149 1104 7 0.01364355192497868 1104 8 0.08004048475751802 1104 9 0.1595811644364673 1104 10-11 0.10904820777933111 1104 12-13 0.15159009039081894 1104 14-15 0.10661868845117084 1104 16-17 0.16525647502649576 1104 18-19 0.09682662601162662 1104 20-21 0.1484048228760244 1104 22-23 0.14469353678102692 1104 24-25 0.028405787435445973 1104 26-27 0.12451446862559834 1104 28-29 0.3923793282728312 1104 30-31 0.33292290017967474 1104 32-33 0.2873126047026915 1104 34-35 0.4728851419434079 1104 36-37 0.4040636495200829 1104 38-39 0.3793075738340832 1104 40-41 0.43498858147410857 1104 42-43 0.46424961026770717 1104 44-45 0.5784260527399141 1104 46-47 0.4599351667222038 1104 48-49 0.7062817245132109 1104 50-51 0.7909693545685812 1104 52-53 0.7888858068733953 1104 54-55 0.627309727036085 1104 56-57 0.8169332671142229 1104 58-59 0.9963192292837917 1104 60-61 1.0267768794759675 1104 62-63 0.8307961205328027 1104 64-65 0.7763938066941236 1104 66-67 0.8176393125449692 1104 68-69 0.9050705542841122 1104 70-71 0.6254331687368087 1104 72-73 0.718987655781163 1104 74-75 0.9065948280608254 1104 76-77 0.7326867362141165 1104 78-79 0.9013855279337264 1104 80-81 0.9414083740742605 1104 82-83 0.7748014265284837 1104 84-85 0.8628266226579839 1104 86-87 0.8498301943160129 1104 88-89 0.7319009865277195 1104 90-91 0.7964917391327546 1104 92-93 0.8301756867061947 1104 94-95 0.7855122929829044 1104 96-97 1.011732150000629 1104 98-99 0.7111145227684261 1104 100-101 0.5806868384268427 1105 1 0.10651709860069047 1105 2 0.2689849828779849 1105 3 0.1622311819019373 1105 4 0.18763723979071756 1105 5 0.16563847149402733 1105 6 0.1662619846314115 1105 7 0.17474312711834727 1105 8 0.1459594333845473 1105 9 0.14323966474532313 1105 10-11 0.2873126777134658 1105 12-13 0.054475255925162 1105 14-15 0.19966523226887745 1105 16-17 0.27743495009082153 1105 18-19 0.33354053162870656 1105 20-21 0.297922376354272 1105 22-23 0.1594831590108683 1105 24-25 0.16733667361576465 1105 26-27 0.25571633954351825 1105 28-29 0.15337255664693572 1105 30-31 0.4065400453772554 1105 32-33 0.4357785261525464 1105 34-35 0.16411467822035775 1105 36-37 0.34373449695817726 1105 38-39 0.1435166657054907 1105 40-41 0.1853627605914454 1105 42-43 0.29825968931988456 1105 44-45 0.21808765457045354 1105 46-47 0.30972084281953727 1105 48-49 0.3566510351705361 1105 50-51 0.39331532698987104 1105 52-53 0.24998658801556672 1105 54-55 0.5507190247227598 1105 56-57 0.5699969354050509 1105 58-59 0.2821735390545186 1105 60-61 0.33104893080830067 1105 62-63 0.3287949154772889 1105 64-65 0.4333924859568583 1105 66-67 0.5847460314217727 1105 68-69 0.560841845645367 1105 70-71 0.42422686260714926 1105 72-73 0.5561242687890662 1105 74-75 0.6736403562016413 1105 76-77 0.4969545622591234 1105 78-79 0.3996168030102005 1105 80-81 0.5242452212595516 1105 82-83 0.5970660108044328 1105 84-85 0.4640119810101382 1105 86-87 0.568726876214857 1105 88-89 0.4678576009559592 1105 90-91 0.6115686451683473 1105 92-93 0.6076151536589514 1105 94-95 0.5524716181160834 1105 96-97 0.5657457039719738 1105 98-99 0.5409676516026174 1105 100-101 0.19940937238435197 1106 1 0.186858658474101 1106 2 0.28123886738504567 1106 3 0.23158040142959635 1106 4 0.12006065440744607 1106 5 0.1699503747574198 1106 6 0.14618161628953175 1106 7 0.17575749566555743 1106 8 0.17012126813062878 1106 9 0.21842122444019196 1106 10-11 0.12144277578024543 1106 12-13 0.23479181925791437 1106 14-15 0.20992720494229644 1106 16-17 0.3101304810664445 1106 18-19 0.3720798409207262 1106 20-21 0.21779451458514387 1106 22-23 0.24865636495110977 1106 24-25 0.29754186843255326 1106 26-27 0.2345236809382314 1106 28-29 0.3188267795000215 1106 30-31 0.3100447500832999 1106 32-33 0.25287973089653093 1106 34-35 0.3569314647365047 1106 36-37 0.3222450044325882 1106 38-39 0.4739781866557138 1106 40-41 0.1611476408089203 1106 42-43 0.4405996445046654 1106 44-45 0.4184407865030977 1106 46-47 0.351974481824854 1106 48-49 0.36127344233739933 1106 50-51 0.26406786793287296 1106 52-53 0.48378905285302665 1106 54-55 0.450062753938802 1106 56-57 0.32790962382763666 1106 58-59 0.4020852365428098 1106 60-61 0.29778065543569454 1106 62-63 0.27036048006433333 1106 64-65 0.33546714303556513 1106 66-67 0.3475681966578321 1106 68-69 0.13978934621448502 1106 70-71 0.3950593032068852 1106 72-73 0.4753712145242517 1106 74-75 0.37224903089398254 1106 76-77 0.24492694108169744 1106 78-79 0.1829193928303603 1106 80-81 0.21418015213965447 1106 82-83 0.28148356908877403 1106 84-85 0.4045489491083032 1106 86-87 0.422492928680807 1106 88-89 0.47694479201433637 1106 90-91 0.5491648930090491 1106 92-93 0.538839515466023 1106 94-95 0.5410446281013108 1106 96-97 0.4355717548310061 1106 98-99 0.574930864283008 1106 100-101 0.3439370828351329 1107 1 0.10424148780123232 1107 2 0.18902080931663434 1107 3 0.14444009816432413 1107 4 0.07625626912270178 1107 5 -0.018244249176419203 1107 6 0.10825730337490569 1107 7 0.06334149669941525 1107 8 0.06362303430621807 1107 9 0.0757217758315889 1107 10-11 0.1909832470475834 1107 12-13 0.2505581671514321 1107 14-15 0.27640648468727846 1107 16-17 0.15918977718086325 1107 18-19 0.21347942737717318 1107 20-21 0.1090077910562357 1107 22-23 0.28249101214677097 1107 24-25 0.23217183310904232 1107 26-27 0.06784355256742458 1107 28-29 0.14973431374659896 1107 30-31 0.3179656168016578 1107 32-33 0.3725192803063706 1107 34-35 0.3359904377700218 1107 36-37 0.4314248097225004 1107 38-39 0.3606333301036386 1107 40-41 0.48765319780039107 1107 42-43 0.42844103332175365 1107 44-45 0.4488561608461623 1107 46-47 0.6209393306229885 1107 48-49 0.5399452567597365 1107 50-51 0.502194644877811 1107 52-53 0.6293962295567397 1107 54-55 0.7001704475719563 1107 56-57 0.49922057842400136 1107 58-59 0.57137693551757 1107 60-61 0.7866505184493917 1107 62-63 0.5600455838809992 1107 64-65 0.5929814662887694 1107 66-67 0.6186061478941554 1107 68-69 0.6841483537099613 1107 70-71 0.5206273871217668 1107 72-73 0.560198797017577 1107 74-75 0.7569656889451579 1107 76-77 0.38810562635782375 1107 78-79 0.6374053711193248 1107 80-81 0.7812409775036606 1107 82-83 0.9520622069797042 1107 84-85 0.7848017060653127 1107 86-87 1.1926531768069388 1107 88-89 1.2402503403903182 1107 90-91 0.7736899640868451 1107 92-93 0.45711889428914887 1107 94-95 0.6589799257665128 1107 96-97 0.6029195837273633 1107 98-99 0.75144342136063 1107 100-101 0.8005446256971993 1108 1 -0.03179555169869985 1108 2 0.11116803634150685 1108 3 -0.09435994173460216 1108 4 0.0012446319369061598 1108 5 -0.006583789008843155 1108 6 -0.028235248826185 1108 7 -0.04071988114338154 1108 8 0.009456921791255013 1108 9 -0.0653059539197045 1108 10-11 -0.12387993289655697 1108 12-13 0.010300154117778959 1108 14-15 -0.02216047900470386 1108 16-17 -0.005219552517239379 1108 18-19 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2316 50-51 -1.2814960071226622 2316 52-53 -1.4946309045947785 2316 54-55 -1.3964307814005608 2316 56-57 -1.2221403516230183 2316 58-59 -1.3866895758300544 2316 60-61 -1.0849640781454681 2316 62-63 -1.0236442193815556 2316 64-65 -1.3083087214520575 2316 66-67 -1.2222936840557317 2316 68-69 -1.440532308645114 2316 70-71 -1.0282137673315646 2316 72-73 -0.8230684408497169 2316 74-75 -1.0941768183964236 2316 76-77 -1.1727976854175637 2316 78-79 -1.457136076997326 2316 80-81 -1.1406528538690885 2316 82-83 -1.2505299447406912 2316 84-85 -1.0615305790766811 2316 86-87 -1.2191173329450606 2316 88-89 -1.191663903129566 2316 90-91 -1.915709087024151 2316 92-93 -1.7372733881368845 2316 94-95 -1.7613214610712582 2316 96-97 -1.606840159784035 2316 98-99 -0.6410697552956783 2316 100-101 -0.2270441310714837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 17.0 5 45.0 6 97.0 7 195.0 8 477.0 9 849.0 10 1359.0 11 2193.0 12 3143.0 13 4358.0 14 5802.0 15 7655.0 16 10370.0 17 13706.0 18 11866.0 19 7713.0 20 7294.0 21 7581.0 22 7782.0 23 8648.0 24 10049.0 25 11661.0 26 14253.0 27 17840.0 28 21747.0 29 26515.0 30 32570.0 31 39883.0 32 49726.0 33 64462.0 34 84697.0 35 114630.0 36 158227.0 37 186785.0 38 89093.0 39 3061.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.864367406160135 19.243005798821528 14.877275654986196 22.015351140032134 2 16.239526116134286 20.78727942602288 38.73975267066377 24.23344178717906 3 19.53787121429142 25.07936322340382 29.828099940173086 25.554665622131672 4 13.52574258004548 16.87595118253546 36.01827439655324 33.58003184086582 5 14.00212611092219 38.00155317177359 32.82424896739411 15.172071749910113 6 32.261810925806664 35.92320036166682 16.734008275856695 15.080980436669819 7 29.451213274159528 33.286046251053484 19.584549205684194 17.678191269102797 8 27.982956898256813 33.60605024265861 18.95056116936022 19.46043168972435 9 27.891145194951466 16.28077142673414 19.658540197474956 36.16954318083943 10-11 25.8575703043477 26.520009080643327 25.965963176627284 21.656457438381697 12-13 26.946419332806553 23.930174529574934 27.016911236711522 22.10649490090699 14-15 23.678403044566537 25.197130032259857 25.8262020961599 25.298264827013707 16-17 22.735957891700917 28.557857013405357 26.34394615251202 22.36223894238171 18-19 22.581584925441263 26.212696883143327 29.574160961515133 21.631557229900274 20-21 23.544081059790802 25.337517726592253 29.626488339543833 21.491912874073112 22-23 22.934619568579862 25.37159431755792 29.8162087271016 21.877577386760617 24-25 23.104612592055272 25.504370570178793 29.79494881636785 21.596068021398086 26-27 22.681803035587972 25.979044349047797 29.621048406111704 21.718104209252523 28-29 22.266034138218963 26.115912884113424 29.758894809505854 21.85915816816176 30-31 22.46016241943577 25.731252831622587 29.746189184054252 22.062395564887392 32-33 22.349164683850425 25.95769537316894 29.603738802203605 22.089401140777028 34-35 22.91178182827941 25.631182421158528 29.39628147386728 22.060754276694784 36-37 23.272280143479946 25.425697291993778 29.21374917120989 22.08827339331638 38-39 22.758343836155007 25.705061280127918 29.43750508495715 22.099089798759927 40-41 22.701657022542797 25.756630964409137 29.437019600354265 22.104692412693804 42-43 22.87798250190848 25.880446109259065 28.878038039032855 22.363533349799603 44-45 22.845844179444494 26.065403572773572 28.675826850026237 22.4129253977557 46-47 23.297306303155132 26.528193351449154 27.95966128810679 22.21483905728892 48-49 23.639197200520105 26.545026543516315 27.46602347092831 22.349752785035275 50-51 23.311272796361838 26.514735578035598 27.402548369574486 22.771443256028082 52-53 23.621339514463642 26.736555140329877 27.20666790406192 22.43543744114456 54-55 23.20336724776698 26.7125733479806 27.216393897837744 22.867665506414674 56-57 23.408775099344975 27.0391786572496 26.962002373755382 22.590043869650035 58-59 23.646596926230806 26.370624638578544 27.128081186402614 22.854697248788035 60-61 23.4513138529309 26.36777164092648 27.705959241498824 22.474955264643796 62-63 23.056436098408398 26.74005254514639 27.81516735672971 22.3883439997155 64-65 23.078122290059376 26.60837716332429 27.489199211937088 22.82430133467925 66-67 23.219224362993483 26.825521864755807 27.15986411956621 22.795389652684506 68-69 23.05886410769936 26.58883984182606 27.50725028828208 22.84504576219249 70-71 23.149004334993993 26.750683767595994 27.349096121110904 22.751215776299112 72-73 23.28120714375146 26.702642977579682 26.971223011068147 23.04492686760071 74-75 23.24286454138817 27.08914018838205 26.73664855731777 22.93134671291201 76-77 23.24049774516161 26.919316392044223 27.032582012834823 22.807603849959346 78-79 23.172346593262038 26.754865898516478 26.903900026795935 23.168887481425543 80-81 23.122312630745114 27.412213822273362 26.75501715336895 22.710456393612574 82-83 23.397082380085664 27.321750805794707 26.47240226207133 22.808764552048295 84-85 23.195148543695943 27.07718075357755 26.43182365143409 23.29584705129242 86-87 23.1987103789358 26.911632311847445 26.547574506892403 23.342082802324356 88-89 23.099546827941744 26.90332751643979 26.441581142741892 23.555544512876576 90-91 23.23948808493721 26.85517566630029 26.65551943749367 23.249816811268836 92-93 23.17465823331188 26.62425955219685 26.69645915047088 23.50462306402039 94-95 22.96444104946451 26.812730914929773 26.61976592825853 23.603062107347185 96-97 23.232195783537517 26.78246771594317 26.49085406846344 23.49448243205587 98-99 23.04215444124539 26.89290638138248 26.117825877263495 23.94711330010864 100-101 22.914264306617387 26.945601068657442 26.172071580951435 23.96806304377374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 367.0 1 253.5 2 193.0 3 271.5 4 254.5 5 236.0 6 367.5 7 709.5 8 1138.5 9 1381.0 10 1313.0 11 1158.0 12 1197.5 13 1330.0 14 1452.0 15 1575.5 16 1703.5 17 1823.5 18 1943.0 19 2033.5 20 2178.0 21 2381.5 22 2607.5 23 3017.5 24 3482.0 25 4004.0 26 4828.0 27 5867.0 28 6994.0 29 8401.0 30 10267.5 31 12267.0 32 14610.0 33 16754.5 34 18446.0 35 19802.5 36 21810.5 37 23882.0 38 24899.0 39 25508.0 40 25968.0 41 28003.5 42 30170.0 43 31903.0 44 33761.5 45 35743.0 46 36076.0 47 36392.5 48 39967.0 49 40363.0 50 37620.0 51 35636.5 52 35056.5 53 35769.5 54 35831.0 55 34178.5 56 31185.0 57 28582.5 58 27311.5 59 25218.5 60 21541.5 61 17991.0 62 15544.5 63 12832.5 64 9596.5 65 7447.0 66 5630.5 67 4654.0 68 3555.5 69 2216.0 70 1640.0 71 1282.5 72 1067.0 73 950.0 74 797.5 75 685.0 76 541.0 77 364.0 78 235.0 79 164.0 80 124.0 81 87.5 82 59.5 83 42.5 84 23.0 85 10.5 86 5.0 87 2.5 88 1.5 89 2.5 90 2.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011302088255668825 2 0.011302088255668825 3 0.0063330666949868416 4 4.871589765374494E-4 5 0.007015089262139271 6 0.0 7 3.897271812299595E-4 8 0.0019486359061497976 9 9.743179530748988E-5 10-11 0.0 12-13 0.0 14-15 5.845907718449392E-4 16-17 0.005163885151296964 18-19 0.0019973518038035425 20-21 5.358748741911944E-4 22-23 4.384430788837044E-4 24-25 5.358748741911944E-4 26-27 9.743179530748988E-5 28-29 1.9486359061497976E-4 30-31 3.897271812299595E-4 32-33 0.003994703607607085 34-35 0.0011204656460361336 36-37 0.0013153292366511133 38-39 0.004628010277105769 40-41 0.0 42-43 0.0018024882131885626 44-45 0.006771509773870546 46-47 0.0 48-49 0.0024845107803409916 50-51 0.0016076246225735827 52-53 9.256020554211539E-4 54-55 1.461476929612348E-4 56-57 0.013348155957126111 58-59 0.0028742379615709513 60-61 0.004433146686490789 62-63 3.4101128357621454E-4 64-65 0.004384430788837044 66-67 0.0015101928272660931 68-69 0.0019973518038035425 70-71 0.005845907718449393 72-73 0.0034588287334158902 74-75 0.009109872861250303 76-77 0.0010230338507286436 78-79 0.008184270805829149 80-81 0.0029229538592246964 82-83 0.004189567198222065 84-85 0.0027280902686097164 86-87 0.0010717497483823887 88-89 0.0037024082216846155 90-91 0.009256020554211538 92-93 0.0036536923240308703 94-95 6.820225671524291E-4 96-97 9.743179530748988E-5 98-99 0.0013640451343048582 100-101 0.0023870789850335018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1026359.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.568575496535075 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38705471835166 39.391715982012734 2 12.936942448562935 13.342793867060939 3 4.343467141344813 6.7195923958547805 4 1.9612844077532807 4.045625722056085 5 1.082197540171158 2.7903692776240456 6 0.6663604854999428 2.061795660264694 7 0.42988174469904306 1.5517872444266372 8 0.3254530564244592 1.342652040864225 9 0.2259110827435941 1.0484921453370313 >10 1.4352207320841452 14.052972624007218 >50 0.13289256287451362 4.73618058557576 >100 0.06917596182587815 6.338108097419738 >500 0.0030240855743110264 0.9848102638337997 >1k 9.450267419721957E-4 1.0814978906868409 >5k 1.8900534839443915E-4 0.511606202975417 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5249 0.5114194935690143 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4648 0.4528629845892129 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2777 0.2705680955688994 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1381 0.13455330931964352 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1274 0.1241281072217421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 9.743179530748988E-5 5 0.0 0.0 0.0 0.0 9.743179530748988E-5 6 0.0 0.0 0.0 0.0 9.743179530748988E-5 7 0.0 0.0 0.0 0.0 9.743179530748988E-5 8 0.0 0.0 0.0 0.0 9.743179530748988E-5 9 0.0 0.0 0.0 0.0 9.743179530748988E-5 10-11 0.0 0.0 0.0 0.0 9.743179530748988E-5 12-13 0.0 0.0 0.0 0.0 9.743179530748988E-5 14-15 0.0 0.0 0.0 9.743179530748988E-5 1.9486359061497976E-4 16-17 0.0 0.0 0.0 9.743179530748988E-5 2.9229538592246963E-4 18-19 0.0 0.0 0.0 9.743179530748988E-5 2.9229538592246963E-4 20-21 0.0 0.0 0.0 9.743179530748988E-5 2.9229538592246963E-4 22-23 0.0 0.0 0.0 1.4614769296123482E-4 2.9229538592246963E-4 24-25 0.0 0.0 0.0 3.410112835762146E-4 2.9229538592246963E-4 26-27 0.0 0.0 0.0 8.281702601136639E-4 2.9229538592246963E-4 28-29 0.0 0.0 0.0 0.002192215394418522 2.9229538592246963E-4 30-31 0.0 0.0 0.0 0.003702408221684615 2.9229538592246963E-4 32-33 0.0 0.0 0.0 0.005602328230180668 2.9229538592246963E-4 34-35 0.0 0.0 0.0 0.008281702601136639 2.9229538592246963E-4 36-37 0.0 0.0 0.0 0.010814929279131375 2.9229538592246963E-4 38-39 0.0 0.0 0.0 0.014078894421932286 2.9229538592246963E-4 40-41 0.0 0.0 0.0 0.01846332521076933 2.9229538592246963E-4 42-43 0.0 0.0 0.0 0.026647596016598482 3.410112835762146E-4 44-45 0.0 0.0 0.0 0.04223668326579686 3.897271812299595E-4 46-47 0.0 0.0 0.0 0.05831292949153269 3.897271812299595E-4 48-49 0.0 0.0 0.0 0.0871527409025497 3.897271812299595E-4 50-51 0.0 0.0 0.0 0.11945138104698258 3.897271812299595E-4 52-53 0.0 0.0 0.0 0.15043469195476436 5.358748741911942E-4 54-55 0.0 0.0 0.0 0.20762715580026092 6.820225671524291E-4 56-57 0.0 0.0 0.0 0.26555035811056366 6.820225671524291E-4 58-59 0.0 0.0 0.0 0.33068351327362067 6.820225671524291E-4 60-61 0.0 0.0 0.0 0.4024907464152407 6.820225671524291E-4 62-63 0.0 0.0 0.0 0.5003609848016143 6.820225671524291E-4 64-65 0.0 0.0 0.0 0.569927286651162 6.820225671524291E-4 66-67 0.0 0.0 0.0 0.6815354081758916 6.820225671524291E-4 68-69 0.0 0.0 0.0 0.8348930539898807 6.820225671524291E-4 70-71 0.0 0.0 0.0 0.9237508513103114 6.820225671524291E-4 72-73 0.0 0.0 0.0 1.0670243063099754 7.79454362459919E-4 74-75 0.0 0.0 0.0 1.255603546127622 7.79454362459919E-4 76-77 0.0 0.0 0.0 1.3864544472255809 7.79454362459919E-4 78-79 0.0 0.0 0.0 1.507903180076367 7.79454362459919E-4 80-81 0.0 0.0 0.0 1.701792452738272 7.79454362459919E-4 82-83 0.0 0.0 0.0 1.9418156804782734 7.79454362459919E-4 84-85 0.0 0.0 0.0 2.2079993452583357 7.79454362459919E-4 86-87 0.0 0.0 0.0 2.6205255665902474 7.79454362459919E-4 88-89 4.871589765374494E-5 0.0 0.0 3.0693938475718534 7.79454362459919E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5070 0.0 30.17841 1 GTATCAA 7270 0.0 29.346773 1 TCGTAGA 85 6.393365E-4 27.95114 2 GTACATG 6370 0.0 26.406586 1 TACATGG 6630 0.0 25.371035 2 ACATGGG 6490 0.0 24.671259 3 GTCTACG 100 0.0016500973 23.758469 1 GAACCGA 120 1.711594E-4 23.748053 6 ATCAACG 9115 0.0 23.35218 3 GTAAGGT 450 0.0 23.224844 4 TCAACGC 9165 0.0 23.221386 4 TAAGGTG 535 0.0 23.086716 5 TATCAAC 9310 0.0 22.967371 2 CAACGCA 9405 0.0 22.628817 5 AGGTAAG 445 0.0 22.423723 2 AACGCAG 9520 0.0 22.401 6 GAGTACT 5030 0.0 22.09668 12-13 CATGGGG 2350 0.0 21.6301 4 AGTACTT 5210 0.0 21.424429 12-13 CATGGGA 4080 0.0 21.423985 4 >>END_MODULE