FastQCFastQC Report
Thu 26 May 2016
SRR936606_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936606_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169561
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53410.45666707422699626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42250.3612466557964912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.21426843063337442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45400.039.8835371
GTATCAA70050.033.854541
GTACATG72800.028.397651
TACATGG74500.027.4921282
GTACACG1754.9330993E-927.1572931
ATCAACG87950.026.7999023
TCAACGC88500.026.3648684
TATCAAC90500.026.2022972
ACATGGG74900.026.0763853
CAACGCA90150.025.8318165
AACGCAG91450.025.5154786
CATGGGG28200.025.1087194
GAGTACT49250.023.53946912-13
ACGCAGA103100.022.5842767
CGCAGAG103550.022.3943528
CATGGGA43900.021.8662454
ATGGGAG16400.021.7341145
GTACTTT53600.021.40426814-15
CCGACTA1353.7856732E-421.1196299
GCAGAGT107850.021.016829
Produced by FastQC (version 0.11.5)