##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936606_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1169561 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.355335035966487 31.0 31.0 34.0 30.0 34.0 2 31.411287654085594 31.0 31.0 34.0 28.0 34.0 3 31.432854720702895 31.0 31.0 34.0 28.0 34.0 4 35.098714816927036 37.0 35.0 37.0 32.0 37.0 5 35.03201885151779 37.0 35.0 37.0 32.0 37.0 6 35.133338919474916 37.0 35.0 37.0 32.0 37.0 7 34.97347893782368 37.0 35.0 37.0 32.0 37.0 8 34.95523961554806 37.0 35.0 37.0 32.0 37.0 9 36.47376494257247 39.0 37.0 39.0 32.0 39.0 10-11 36.33090236422042 39.0 36.0 39.0 32.0 39.0 12-13 36.23957621705922 39.0 35.0 39.0 32.0 39.0 14-15 37.28951846034538 39.5 37.0 41.0 32.0 41.0 16-17 37.23438623551914 39.0 37.0 41.0 31.5 41.0 18-19 37.20351653312653 39.0 36.5 41.0 31.0 41.0 20-21 37.10581748194409 39.0 36.0 41.0 31.0 41.0 22-23 36.993000365094254 39.0 36.0 41.0 31.0 41.0 24-25 36.839261483582305 39.0 36.0 41.0 30.5 41.0 26-27 36.64083574948207 39.0 36.0 40.5 30.0 41.0 28-29 36.71850164292414 39.0 36.0 41.0 30.0 41.0 30-31 36.64233759504634 39.0 36.0 41.0 30.0 41.0 32-33 36.46585599212012 39.0 35.0 40.5 30.0 41.0 34-35 36.27097859795256 39.0 35.0 40.0 30.0 41.0 36-37 36.028844156055136 38.0 35.0 40.0 29.5 41.0 38-39 35.83571228862796 38.0 35.0 40.0 28.5 41.0 40-41 35.64998533637835 38.0 35.0 40.0 27.5 41.0 42-43 35.35615884934603 38.0 34.5 40.0 27.0 41.0 44-45 35.048814469702734 38.0 34.0 40.0 25.5 41.0 46-47 34.63498312614733 38.0 33.5 40.0 24.0 41.0 48-49 34.3521611955255 38.0 33.0 40.0 23.5 41.0 50-51 33.626326886754946 37.5 32.0 39.5 22.5 40.5 52-53 33.79783311858039 37.5 33.0 39.5 23.0 40.5 54-55 34.14710904347871 38.0 33.0 40.0 23.0 41.0 56-57 34.05297970777069 38.0 33.0 40.0 23.0 41.0 58-59 33.81108082434349 37.5 33.0 40.0 22.0 41.0 60-61 33.49045411055943 37.0 33.0 40.0 21.5 41.0 62-63 33.15939185728662 36.5 32.5 40.0 20.0 41.0 64-65 32.83599743835508 36.0 32.0 39.0 20.0 41.0 66-67 32.47347594524783 35.5 32.0 39.0 18.5 41.0 68-69 32.08843147129564 35.0 31.0 39.0 17.5 40.0 70-71 31.685533717352065 35.0 31.0 38.0 14.5 40.0 72-73 31.282397839873255 35.0 31.0 37.0 12.0 39.5 74-75 30.863118725744105 35.0 30.5 37.0 8.0 39.0 76-77 30.448769666567202 34.5 30.0 36.0 7.0 39.0 78-79 29.9968453975466 34.0 29.5 36.0 4.5 37.5 80-81 29.608913087902213 34.0 29.0 35.0 2.0 37.0 82-83 29.221551077712064 34.0 29.0 35.0 2.0 37.0 84-85 28.845623272321838 34.0 29.0 35.0 2.0 36.0 86-87 28.50847924990659 34.0 29.0 35.0 2.0 36.0 88-89 28.134297826278406 34.0 27.5 35.0 2.0 35.5 90-91 27.79870395815182 34.0 27.0 35.0 2.0 35.0 92-93 27.4291764174763 33.0 26.5 35.0 2.0 35.0 94-95 27.001352216771934 33.0 25.5 35.0 2.0 35.0 96-97 26.405201182323967 33.0 25.0 35.0 2.0 35.0 98-99 25.736438287528397 33.0 23.5 35.0 2.0 35.0 100-101 23.708676588908148 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13865398795955386 1101 2 0.2588187815964744 1101 3 0.26480533845760945 1101 4 0.08138737854347511 1101 5 0.20951009939864917 1101 6 0.08788000466211798 1101 7 0.08357742799366719 1101 8 0.19813795134724188 1101 9 0.48188822630307726 1101 10-11 0.17197136331026996 1101 12-13 0.34084961124582236 1101 14-15 0.39497299758416204 1101 16-17 0.4850289122138278 1101 18-19 0.5657780180660836 1101 20-21 0.5302380442809849 1101 22-23 0.4757865045506193 1101 24-25 0.37765223403162906 1101 26-27 0.36177669632524356 1101 28-29 0.4870180971384954 1101 30-31 0.27903635281522554 1101 32-33 0.36929702719884716 1101 34-35 0.40775021383634424 1101 36-37 0.4247671515610705 1101 38-39 0.4179439726460359 1101 40-41 0.2407105017367286 1101 42-43 0.011175406572675683 1101 44-45 0.38821467031750245 1101 46-47 0.6011924422641712 1101 48-49 0.5150425130710374 1101 50-51 0.6201515837067859 1101 52-53 0.6960273540518216 1101 54-55 0.7477566993586962 1101 56-57 0.5674818105018389 1101 58-59 0.5163840929513484 1101 60-61 0.4885231727684811 1101 62-63 0.6175468046012114 1101 64-65 0.754120554242057 1101 66-67 0.6279991161249754 1101 68-69 0.6458523155437419 1101 70-71 0.7270488614013892 1101 72-73 0.5559910579997229 1101 74-75 0.5349848858583428 1101 76-77 0.5667531337800611 1101 78-79 0.6265177764006431 1101 80-81 0.31960614975154655 1101 82-83 0.5304761125324795 1101 84-85 0.46048439487071136 1101 86-87 0.47985462278210633 1101 88-89 0.5663774166171329 1101 90-91 0.42914636026669584 1101 92-93 0.04623642900661551 1101 94-95 -0.05474398378618872 1101 96-97 -0.12727087680963578 1101 98-99 -0.356247938469604 1101 100-101 0.05496125892300796 1102 1 0.1233046583900439 1102 2 0.01790355444271441 1102 3 0.11979971180060645 1102 4 0.1452271533924261 1102 5 0.13302465135356556 1102 6 0.13649336749566743 1102 7 -0.18490312030339595 1102 8 -0.07117599915936523 1102 9 0.26148804489372424 1102 10-11 0.27087151610950144 1102 12-13 0.30129585494895395 1102 14-15 0.12605111168979732 1102 16-17 0.1618723046535564 1102 18-19 0.07335625614891228 1102 20-21 -0.029498032656924522 1102 22-23 -0.32408782284664284 1102 24-25 -0.10591549036178094 1102 26-27 0.006381516388778152 1102 28-29 -0.045406176111463026 1102 30-31 0.010798990541161402 1102 32-33 0.06608432261000274 1102 34-35 -0.06133363712823581 1102 36-37 -0.15110980160946497 1102 38-39 -0.2439424197449398 1102 40-41 -0.3363927129811657 1102 42-43 -0.08093196191084928 1102 44-45 0.1445205527701745 1102 46-47 0.08146099592722322 1102 48-49 0.3985534940590867 1102 50-51 0.43941324127119685 1102 52-53 0.22126439419712085 1102 54-55 0.015984260017447127 1102 56-57 0.3190768247115372 1102 58-59 0.2035546792045082 1102 60-61 -0.07270645841527568 1102 62-63 0.12829811695802107 1102 64-65 0.17460428779918402 1102 66-67 0.2572551364360365 1102 68-69 0.19228707069409268 1102 70-71 0.40947970438268655 1102 72-73 0.19516260428136079 1102 74-75 0.15432961873518636 1102 76-77 0.16479975132208935 1102 78-79 0.22378132435226306 1102 80-81 0.3668570880575288 1102 82-83 0.10206393182672002 1102 84-85 0.008254818787925444 1102 86-87 0.07854496803208733 1102 88-89 -0.039005312618808574 1102 90-91 -0.20723013620154163 1102 92-93 -0.23308099709980112 1102 94-95 -0.17167645966361533 1102 96-97 -0.3431286717958706 1102 98-99 -0.30187281528714394 1102 100-101 -0.12896631720640173 1103 1 0.20394744026570066 1103 2 0.4168669013512698 1103 3 0.32724595678109836 1103 4 0.3439598165086508 1103 5 0.2870206072673298 1103 6 0.22505230578062907 1103 7 0.15852694833215253 1103 8 0.03848509502227415 1103 9 0.06083785835312483 1103 10-11 0.2840246217968527 1103 12-13 0.41478046178972505 1103 14-15 0.5023402353761881 1103 16-17 0.31494666457722076 1103 18-19 0.4189363831673987 1103 20-21 0.3111938763226547 1103 22-23 0.20982725134241065 1103 24-25 0.5416731520213744 1103 26-27 0.348937407369327 1103 28-29 0.4441897000597521 1103 30-31 0.25818343504889185 1103 32-33 0.43837805997223 1103 34-35 0.34761504284784195 1103 36-37 0.3771066709220747 1103 38-39 0.31758621637273166 1103 40-41 0.1540344792268442 1103 42-43 0.29171762656065425 1103 44-45 0.5702716990692664 1103 46-47 0.430543956654013 1103 48-49 0.3809097438810909 1103 50-51 -0.1886870349797647 1103 52-53 0.14774589677451644 1103 54-55 0.2591635668268921 1103 56-57 0.34455357121567687 1103 58-59 0.3562027013045821 1103 60-61 0.4377171738576706 1103 62-63 0.29722116540161636 1103 64-65 0.38477616146421667 1103 66-67 0.3660212629418922 1103 68-69 0.10418893040417032 1103 70-71 0.3576051849739166 1103 72-73 0.3096457017096661 1103 74-75 -0.036300774566441874 1103 76-77 0.09837406852654595 1103 78-79 0.21413859943556446 1103 80-81 0.4041884183340656 1103 82-83 0.26088260297415644 1103 84-85 -0.029635136499290837 1103 86-87 0.11972615448724255 1103 88-89 0.21986519118320302 1103 90-91 0.20911452247188222 1103 92-93 0.04077540935934465 1103 94-95 0.07209094841886099 1103 96-97 0.2837165137479438 1103 98-99 0.2998678505631105 1103 100-101 0.30635849126909065 1104 1 0.2958075286541053 1104 2 0.28370552173722174 1104 3 0.2603340463647932 1104 4 0.22052116152225665 1104 5 0.3117102255615336 1104 6 0.2361225604084396 1104 7 0.3431634077631358 1104 8 0.3661358821823626 1104 9 0.6984120527132518 1104 10-11 0.5093400362944394 1104 12-13 0.42370485982063855 1104 14-15 0.4927544970499014 1104 16-17 0.3242982208108103 1104 18-19 0.4231367197353464 1104 20-21 0.4477997300885974 1104 22-23 0.3967363308133329 1104 24-25 0.5136038567695067 1104 26-27 0.5108692130406496 1104 28-29 0.41688476006006425 1104 30-31 0.35679768728613226 1104 32-33 0.18094918747485877 1104 34-35 0.14929332163305276 1104 36-37 0.13637817431134636 1104 38-39 0.37070135721226904 1104 40-41 0.15968976444460026 1104 42-43 0.28599251135690906 1104 44-45 0.13632866398126708 1104 46-47 0.15965629800312087 1104 48-49 0.15623726853389996 1104 50-51 -0.08470861307453958 1104 52-53 -0.03074866938310805 1104 54-55 0.2697272395066932 1104 56-57 0.14933173519033005 1104 58-59 0.16659809000910997 1104 60-61 0.20032935006829433 1104 62-63 0.1953886000932883 1104 64-65 0.20948575365080302 1104 66-67 0.21076073732970002 1104 68-69 0.4107762257504106 1104 70-71 0.27436784165888284 1104 72-73 0.1309734150921713 1104 74-75 -0.046391050269065204 1104 76-77 0.29317425096524374 1104 78-79 0.19910093213455937 1104 80-81 0.4588800811999256 1104 82-83 0.23975903220838646 1104 84-85 0.4118449761094247 1104 86-87 0.41005658872281003 1104 88-89 0.24142724393315262 1104 90-91 0.2144892295476737 1104 92-93 -0.08054585575868956 1104 94-95 -0.0453654471585736 1104 96-97 0.03969861588216261 1104 98-99 -0.22553154864737124 1104 100-101 -0.13447412122166824 1105 1 0.12103427402010425 1105 2 0.2436749544323682 1105 3 0.08750751581983351 1105 4 0.26542916287331053 1105 5 0.35458431420232017 1105 6 0.29790558969282444 1105 7 0.49035794902276564 1105 8 0.08717808660377813 1105 9 0.4255497675068156 1105 10-11 0.4830609096167038 1105 12-13 0.2812811480325266 1105 14-15 0.34511650273987726 1105 16-17 0.4696196720690722 1105 18-19 0.2512364767062252 1105 20-21 0.09067495437948736 1105 22-23 -0.14485117615625853 1105 24-25 -0.17449735090279717 1105 26-27 0.015259233387105553 1105 28-29 0.16556410888561857 1105 30-31 0.1375653794326368 1105 32-33 -0.001736984043468226 1105 34-35 0.024536478974809484 1105 36-37 -0.10895412057375609 1105 38-39 -0.23292964588915765 1105 40-41 -0.3145980180850003 1105 42-43 -0.18090731770943336 1105 44-45 -0.056838825360102874 1105 46-47 -0.09986228159899468 1105 48-49 0.25023337948178437 1105 50-51 0.20423740837679816 1105 52-53 0.10598177197449132 1105 54-55 0.010143451608037424 1105 56-57 0.33985407562598624 1105 58-59 0.03231131124767472 1105 60-61 0.08197537598895366 1105 62-63 -0.0954770363799895 1105 64-65 0.029617276586904495 1105 66-67 -0.4130029609918111 1105 68-69 -0.1519765021910544 1105 70-71 0.15091095203601412 1105 72-73 0.1637160394110566 1105 74-75 0.041855374081617214 1105 76-77 0.2379563783236449 1105 78-79 0.053118239442209614 1105 80-81 0.03714835399334149 1105 82-83 0.07948546508743348 1105 84-85 -0.008429272401592414 1105 86-87 0.09539871613982243 1105 88-89 0.04359223264625456 1105 90-91 0.018247059122494136 1105 92-93 0.07927546618199699 1105 94-95 0.0399165640704382 1105 96-97 0.03424951022494227 1105 98-99 -0.24186567742423293 1105 100-101 0.1930766771403576 1106 1 0.11465356736072962 1106 2 0.08541304100664249 1106 3 0.017466940413395804 1106 4 0.11370703949408778 1106 5 0.25185987405698285 1106 6 0.2766368722523538 1106 7 0.3177611595815151 1106 8 0.31805608061712576 1106 9 0.40738454752374764 1106 10-11 0.31004789769276186 1106 12-13 0.30231078325613936 1106 14-15 0.31531625296067034 1106 16-17 0.15011760307076827 1106 18-19 0.2679454894201001 1106 20-21 0.20359741677089715 1106 22-23 0.4268243467618049 1106 24-25 0.0601753304568291 1106 26-27 0.24375783363615255 1106 28-29 0.02520021935166028 1106 30-31 0.25247181280138165 1106 32-33 0.1209976776666224 1106 34-35 0.05705257473935177 1106 36-37 0.396626826356389 1106 38-39 0.24295733542128772 1106 40-41 0.10498121549878192 1106 42-43 0.1741702308704305 1106 44-45 -0.17844184648162553 1106 46-47 0.06635174407011846 1106 48-49 0.20557414706827615 1106 50-51 0.256741783169943 1106 52-53 0.09907238733462265 1106 54-55 0.29591161408519895 1106 56-57 0.28013723491235254 1106 58-59 0.3242142411248139 1106 60-61 0.3261402613483426 1106 62-63 0.48898412807383096 1106 64-65 0.32432222261741117 1106 66-67 0.48874293346825226 1106 68-69 0.3230259206306485 1106 70-71 0.36202603515251397 1106 72-73 0.4841803800469897 1106 74-75 0.2590556253236542 1106 76-77 0.2481020584782634 1106 78-79 0.5467117045892316 1106 80-81 0.3382216640066318 1106 82-83 0.5158665100275783 1106 84-85 0.334093716976394 1106 86-87 0.3382132212533442 1106 88-89 0.34244614655471395 1106 90-91 0.1367918392674241 1106 92-93 0.03938964965451319 1106 94-95 0.2926282958302515 1106 96-97 0.43143661423073354 1106 98-99 0.26697327898875045 1106 100-101 0.7259726530620796 1107 1 0.30561564857884704 1107 2 0.46206797609909955 1107 3 0.4556565926735665 1107 4 0.34888133936172494 1107 5 0.4829465495069769 1107 6 0.44664181671164016 1107 7 0.29447843785629857 1107 8 0.6245304047463947 1107 9 0.5880991600900458 1107 10-11 0.7140858471855722 1107 12-13 0.6871471093693415 1107 14-15 0.9074405892207551 1107 16-17 0.6874275408197192 1107 18-19 0.8820008827195807 1107 20-21 0.8816159938107901 1107 22-23 0.9015496375175118 1107 24-25 0.9751321662485708 1107 26-27 1.0006762377735257 1107 28-29 0.8444122844465412 1107 30-31 0.7233513276915744 1107 32-33 0.7700332009961741 1107 34-35 0.9557326190859285 1107 36-37 1.0726238566097237 1107 38-39 1.083946902919294 1107 40-41 1.1025558200188144 1107 42-43 0.9813113660077235 1107 44-45 0.9935021091472294 1107 46-47 1.1949833112180315 1107 48-49 1.0252107907324586 1107 50-51 1.1410928766325057 1107 52-53 1.0162645947439444 1107 54-55 1.326403725983532 1107 56-57 1.0546004079039264 1107 58-59 1.2172381004441988 1107 60-61 1.1240458528868729 1107 62-63 1.0994079542254198 1107 64-65 1.137194784553671 1107 66-67 1.2113480185835854 1107 68-69 1.1756188442187891 1107 70-71 1.0979619660160758 1107 72-73 1.0898735717415242 1107 74-75 0.9495988862713318 1107 76-77 1.0472433809522173 1107 78-79 0.909983201470542 1107 80-81 1.032197638848242 1107 82-83 1.1548422852481615 1107 84-85 1.1045356779305209 1107 86-87 1.145682195539088 1107 88-89 1.1801500287230269 1107 90-91 1.1945425863107282 1107 92-93 1.313638613928493 1107 94-95 1.329515958329445 1107 96-97 1.5942302490839495 1107 98-99 1.704299570306464 1107 100-101 1.9270608947693617 1108 1 0.16420071032633032 1108 2 -0.15529221247238212 1108 3 -0.06788276194663467 1108 4 0.1845986378080724 1108 5 0.30583787372518145 1108 6 0.31405065226590523 1108 7 0.4246535564760521 1108 8 0.045694419232148675 1108 9 0.26113253349797816 1108 10-11 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-2.2420951754142706 2316 68-69 -1.8839407259284435 2316 70-71 -1.5146469779676828 2316 72-73 -1.3769972326811093 2316 74-75 -1.8053247471691805 2316 76-77 -2.641471221067306 2316 78-79 -2.9411813356761876 2316 80-81 -2.832103638863721 2316 82-83 -2.179654527523322 2316 84-85 -2.6248742617095786 2316 86-87 -2.652410050751879 2316 88-89 -2.8884987838228042 2316 90-91 -2.989361204516822 2316 92-93 -2.9929505579562026 2316 94-95 -3.013654064799116 2316 96-97 -3.081273572769721 2316 98-99 -3.632372596444821 2316 100-101 -4.574929360208916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2522.0 3 1080.0 4 1025.0 5 1170.0 6 1325.0 7 1607.0 8 1925.0 9 2496.0 10 3168.0 11 4010.0 12 4974.0 13 5941.0 14 6838.0 15 8468.0 16 10627.0 17 13178.0 18 11419.0 19 7742.0 20 8064.0 21 8468.0 22 9402.0 23 10812.0 24 12491.0 25 13869.0 26 16390.0 27 19447.0 28 22994.0 29 27989.0 30 33805.0 31 41295.0 32 51554.0 33 65921.0 34 86489.0 35 117201.0 36 161028.0 37 201644.0 38 149800.0 39 21377.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69354526999177 18.239601985965525 14.430317614241762 22.63653512980094 2 15.765270435970796 19.71120547387647 39.88287121990762 24.640652870245116 3 19.117965206014766 24.73665083308037 29.527001894044663 26.618382066860203 4 12.744376047234274 16.12879773442438 36.6742191556095 34.45260706273184 5 13.990995792162423 37.186625594841445 33.36552584282927 15.456852770166869 6 32.09606893501325 35.1448280346215 17.600432554888528 15.158670475476722 7 29.37686982670587 32.70573866814457 20.095943812402393 17.821447692747167 8 27.502773138063365 33.04639799940646 19.620390112319576 19.830438750210604 9 27.641514435780827 16.15608533025023 19.903522019247706 36.29887821472123 10-11 25.418426684869917 26.024488846232153 26.58884948273001 21.968234986167925 12-13 26.605973874669925 23.52130819968573 27.3524302065682 22.520287719076144 14-15 23.47618909160568 24.757897203374903 25.966615298570645 25.799298406448777 16-17 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29.231419070575164 22.68090556887062 42-43 22.582014590251962 25.608611189963987 28.63285645394631 23.176517765837744 44-45 22.486598426482765 25.85136704813964 28.539590806856168 23.122443718521428 46-47 22.826547006369207 26.089259559157373 28.034736942010184 23.04945649246323 48-49 22.96105055386474 26.31805529370371 27.475461810458597 23.24543234197295 50-51 22.793541756445247 26.345695416333204 27.569975990642508 23.29078683657904 52-53 23.113538064410218 26.32519929665286 27.385478438083695 23.175784200853233 54-55 22.687033226552682 26.248416566804742 27.440646104871906 23.623904101770666 56-57 22.892738078849874 26.568160617132047 27.136675219894336 23.402426084123746 58-59 23.076045012915433 26.064849927022028 27.249641095447664 23.60946396461488 60-61 22.961017618681993 26.154457169143107 27.858910319660314 23.025614892514582 62-63 22.749919413242907 26.3144619241467 27.82423451131079 23.111384151299603 64-65 22.620424353399535 26.322026847755126 27.682584443795555 23.374964355049784 66-67 22.587690984350164 26.54161960372217 27.29114954430529 23.579539867622376 68-69 22.54076020154041 26.274302742428873 27.74596673343352 23.438970322597193 70-71 22.656307451828113 26.31945738966256 27.563110432354133 23.461124726155195 72-73 22.697369264852878 26.398414393585067 27.328453272750124 23.575763068811934 74-75 22.53504326780637 26.78033737816033 27.158484453217284 23.52613490081602 76-77 22.710636178440346 26.541045597669132 27.254627422730216 23.4936908011603 78-79 22.64516631185322 26.42147047578644 27.34200395326897 23.591359259091362 80-81 22.555564060143496 26.89985961728499 27.114046951882038 23.430529370689474 82-83 22.752028510951757 26.797678183829117 27.025849593271744 23.424443711947383 84-85 22.557650145194224 26.53407361295905 26.897700492873682 24.010575748973036 86-87 22.666096047076973 26.42846576581738 27.04360622459895 23.861831962506702 88-89 22.61138204847458 26.478078870491178 26.943748158934653 23.96679092209958 90-91 22.525352116289234 26.37723196534377 27.27784243421362 23.819573484153377 92-93 22.552701329275397 26.239517563119097 27.30134731889483 23.906433788710675 94-95 22.320450964841587 26.228171621451185 27.281320743826914 24.17005666988031 96-97 22.43714576556109 26.256860236090024 27.164504154084845 24.141489844264036 98-99 22.26341561035559 26.384878235052895 26.897500730149243 24.454205424442275 100-101 22.15498278715133 26.220884751969457 27.190141992635837 24.433990468243376 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 194.5 2 188.5 3 247.0 4 222.0 5 236.5 6 420.0 7 775.0 8 1241.5 9 1527.5 10 1421.5 11 1248.5 12 1258.5 13 1344.5 14 1504.5 15 1621.0 16 1630.5 17 1682.0 18 1822.5 19 1922.0 20 2077.5 21 2362.5 22 2655.0 23 3036.5 24 3581.5 25 4345.0 26 5301.0 27 6429.5 28 7615.5 29 9185.0 30 11274.0 31 13724.0 32 16617.0 33 18933.0 34 20893.5 35 22500.0 36 24849.5 37 27264.5 38 28313.0 39 29037.5 40 30011.5 41 32371.5 42 34353.0 43 36311.0 44 38727.5 45 40788.0 46 41063.5 47 41707.5 48 45799.5 49 46238.5 50 42949.0 51 40690.5 52 39785.5 53 40821.0 54 41878.5 55 40216.5 56 36771.0 57 33474.0 58 31362.5 59 28395.5 60 24359.0 61 20962.5 62 17823.0 63 14428.0 64 11041.5 65 8814.0 66 6753.0 67 5314.0 68 3932.5 69 2499.5 70 1911.5 71 1560.5 72 1264.0 73 1117.5 74 913.5 75 742.5 76 582.0 77 374.5 78 249.0 79 176.0 80 121.5 81 79.5 82 55.5 83 39.5 84 25.5 85 14.0 86 11.0 87 11.5 88 9.5 89 6.5 90 4.0 91 3.5 92 4.5 93 3.5 94 1.5 95 2.0 96 2.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013252835893125712 2 0.006754671197141491 3 0.009063229707556937 4 0.03505588849149381 5 0.04719719621293802 6 0.05916749960027737 7 0.02864322596256202 8 0.026334667452146573 9 0.01496287923417419 10-11 0.0460856680412565 12-13 0.043093092194421666 14-15 0.01906698325269054 16-17 0.022743576435944768 18-19 0.029327243298981412 20-21 0.022786327519470982 22-23 0.013979604313071313 24-25 0.0027360693456775663 26-27 0.002778820429203778 28-29 0.001282532505786359 30-31 0.005985151693669677 32-33 0.0016672922575222667 34-35 0.0015390390069436309 36-37 0.005685894108986192 38-39 0.006198907111300736 40-41 0.0035910910162018058 42-43 0.0011115281716815113 44-45 0.001325283589312571 46-47 0.0023940606774678704 48-49 5.557640858407557E-4 50-51 0.0014535368398912072 52-53 8.977727540504514E-4 54-55 0.0025223139280465064 56-57 0.001881047675153327 58-59 0.002565065011572718 60-61 5.985151693669676E-4 62-63 0.0011970303387339352 64-65 0.00346283776562317 66-67 0.0023940606774678704 68-69 0.0058568984430910406 70-71 0.008806723206399667 72-73 0.0034200866820969576 74-75 0.003633842099728018 76-77 0.0032918334315183216 78-79 0.034371871155074424 80-81 0.022016808015999166 82-83 0.0038903486008852895 84-85 0.007951701535875428 86-87 0.0061561560277745234 88-89 0.005258383273724072 90-91 0.01128628605091996 92-93 0.01406510648012374 94-95 0.0058568984430910406 96-97 0.004232357269094985 98-99 0.023897855691152492 100-101 0.04458938011783909 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1169561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.03531094880083 #Duplication Level Percentage of deduplicated Percentage of total 1 77.42500246494501 41.06259080940029 2 12.415847292363251 13.169566436866237 3 4.129959626860914 6.57101079049686 4 1.8907759585045592 4.011115635752248 5 1.0046759584447573 2.6641650929451104 6 0.6264846113218004 1.9935483699654188 7 0.42469343106469715 1.5766623722100526 8 0.30566359152705 1.2968770897891473 9 0.2118374948740303 1.0111380670133279 >10 1.3699604976218813 13.723459643840021 >50 0.12545715055317816 4.633702163207864 >100 0.06673344428652841 6.3236494119997255 >500 0.0021005671789353065 0.7398955015928979 >1k 6.463283627493251E-4 0.7649186063491089 >5k 1.6158209068733127E-4 0.45770000857165477 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5341 0.45666707422699626 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.3612466557964912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2506 0.21426843063337442 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 8.550216705242395E-5 3 0.0 0.0 0.0 0.0 8.550216705242395E-5 4 0.0 0.0 0.0 0.0 8.550216705242395E-5 5 0.0 0.0 0.0 0.0 8.550216705242395E-5 6 0.0 0.0 0.0 0.0 8.550216705242395E-5 7 0.0 0.0 0.0 0.0 1.710043341048479E-4 8 0.0 0.0 0.0 0.0 1.710043341048479E-4 9 0.0 0.0 0.0 0.0 1.710043341048479E-4 10-11 0.0 0.0 0.0 0.0 1.710043341048479E-4 12-13 0.0 0.0 0.0 0.0 1.710043341048479E-4 14-15 0.0 0.0 0.0 0.0 1.710043341048479E-4 16-17 0.0 0.0 0.0 0.0 1.710043341048479E-4 18-19 0.0 0.0 0.0 0.0 1.710043341048479E-4 20-21 0.0 8.550216705242395E-5 0.0 8.550216705242395E-5 1.710043341048479E-4 22-23 0.0 8.550216705242395E-5 0.0 2.992575846834838E-4 1.710043341048479E-4 24-25 0.0 8.550216705242395E-5 0.0 5.130130023145436E-4 1.710043341048479E-4 26-27 0.0 8.550216705242395E-5 0.0 8.550216705242395E-4 1.710043341048479E-4 28-29 0.0 8.550216705242395E-5 0.0 0.001410785756364995 1.710043341048479E-4 30-31 0.0 8.550216705242395E-5 0.0 0.0034200866820969576 1.710043341048479E-4 32-33 0.0 8.550216705242395E-5 0.0 0.005685894108986192 1.710043341048479E-4 34-35 0.0 8.550216705242395E-5 0.0 0.007780697201770579 2.565065011572718E-4 36-37 0.0 8.550216705242395E-5 0.0 0.010132006795712238 2.565065011572718E-4 38-39 0.0 8.550216705242395E-5 0.0 0.012568818556706319 2.992575846834838E-4 40-41 0.0 8.550216705242395E-5 0.0 0.015903403071750855 3.420086682096958E-4 42-43 0.0 8.550216705242395E-5 0.0 0.02295733185357583 3.847597517359077E-4 44-45 0.0 8.550216705242395E-5 0.0 0.03381610706923367 4.275108352621197E-4 46-47 0.0 8.550216705242395E-5 0.0 0.046513178876518624 4.275108352621197E-4 48-49 0.0 8.550216705242395E-5 0.0 0.07062478998530218 4.275108352621197E-4 50-51 0.0 8.550216705242395E-5 0.0 0.09610443576692451 4.275108352621197E-4 52-53 0.0 8.550216705242395E-5 0.0 0.12226809888496624 4.275108352621197E-4 54-55 0.0 8.550216705242395E-5 0.0 0.17010656135079744 4.275108352621197E-4 56-57 0.0 8.550216705242395E-5 0.0 0.21238738295822107 4.275108352621197E-4 58-59 0.0 8.550216705242395E-5 0.0 0.26625374820124814 4.275108352621197E-4 60-61 0.0 8.550216705242395E-5 0.0 0.3267465313908381 4.275108352621197E-4 62-63 0.0 8.550216705242395E-5 0.0 0.406391800000171 5.130130023145436E-4 64-65 0.0 8.550216705242395E-5 0.0 0.46325074109003295 5.130130023145436E-4 66-67 0.0 8.550216705242395E-5 0.0 0.5632027743743164 5.985151693669676E-4 68-69 0.0 8.550216705242395E-5 0.0 0.700048992741721 5.985151693669676E-4 70-71 0.0 8.550216705242395E-5 0.0 0.7782407245111627 5.985151693669676E-4 72-73 0.0 8.550216705242395E-5 0.0 0.8979437583845562 5.985151693669676E-4 74-75 0.0 8.550216705242395E-5 0.0 1.0508216330742903 5.985151693669676E-4 76-77 0.0 8.550216705242395E-5 0.0 1.1536807400383564 5.985151693669676E-4 78-79 0.0 8.550216705242395E-5 0.0 1.2551718123295834 5.985151693669676E-4 80-81 0.0 8.550216705242395E-5 0.0 1.41501811363409 6.840173364193916E-4 82-83 0.0 8.550216705242395E-5 0.0 1.6212920916480629 7.695195034718155E-4 84-85 0.0 8.550216705242395E-5 0.0 1.8623227005688459 7.695195034718155E-4 86-87 0.0 8.550216705242395E-5 0.0 2.215874161330619 7.695195034718155E-4 88-89 0.0 1.282532505786359E-4 0.0 2.6159815520524368 7.695195034718155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4540 0.0 39.883537 1 GTATCAA 7005 0.0 33.85454 1 GTACATG 7280 0.0 28.39765 1 TACATGG 7450 0.0 27.492128 2 GTACACG 175 4.9330993E-9 27.157293 1 ATCAACG 8795 0.0 26.799902 3 TCAACGC 8850 0.0 26.364868 4 TATCAAC 9050 0.0 26.202297 2 ACATGGG 7490 0.0 26.076385 3 CAACGCA 9015 0.0 25.831816 5 AACGCAG 9145 0.0 25.515478 6 CATGGGG 2820 0.0 25.108719 4 GAGTACT 4925 0.0 23.539469 12-13 ACGCAGA 10310 0.0 22.584276 7 CGCAGAG 10355 0.0 22.394352 8 CATGGGA 4390 0.0 21.866245 4 ATGGGAG 1640 0.0 21.734114 5 GTACTTT 5360 0.0 21.404268 14-15 CCGACTA 135 3.7856732E-4 21.119629 9 GCAGAGT 10785 0.0 21.01682 9 >>END_MODULE