FastQCFastQC Report
Thu 26 May 2016
SRR936606_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936606_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169561
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.5124144871451767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49600.42409074858002277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.27480396490649056No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14750.1261156964023253No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.11405989084793354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55850.033.2824971
GTATCAA84550.031.8696561
GTACATG70450.025.3623851
ATCAACG105900.025.3389053
TCAACGC106150.025.2323284
TATCAAC108150.024.9434852
CAACGCA107850.024.8335385
AACGCAG109700.024.4580296
TACATGG74000.024.0677172
GAGTACT60500.023.31106812-13
ACATGGG73350.023.1802583
AGTACTT63550.022.6032512-13
TGCACCG3800.022.4940975
ACGCAGA122400.021.9591067
GTACTTT63850.021.90208614-15
CGCAGAG124100.021.6965628
TAGTACT2005.397651E-721.3703044
TACTTTT68400.020.75755314-15
GCAGAGT129100.020.5619959
ATGGGAT15450.020.2859385
Produced by FastQC (version 0.11.5)