##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936606_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1169561 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49286783673532 31.0 31.0 34.0 30.0 34.0 2 31.73025263325299 31.0 31.0 34.0 30.0 34.0 3 31.988749624859242 33.0 31.0 34.0 30.0 34.0 4 35.80565699437652 37.0 35.0 37.0 35.0 37.0 5 35.60835390372969 37.0 35.0 37.0 33.0 37.0 6 35.71623284292141 37.0 35.0 37.0 33.0 37.0 7 35.6516068849765 37.0 35.0 37.0 33.0 37.0 8 35.62055164288139 37.0 35.0 37.0 33.0 37.0 9 37.25669033081643 39.0 37.0 39.0 34.0 39.0 10-11 37.140669875277986 39.0 37.0 39.0 33.0 39.0 12-13 37.01662204878583 39.0 37.0 39.0 33.0 39.0 14-15 38.129219852577165 40.0 37.0 41.0 33.0 41.0 16-17 38.044128095926595 40.0 37.0 41.0 33.0 41.0 18-19 38.10211352806737 40.0 37.5 41.0 33.0 41.0 20-21 38.162145454576546 40.0 37.0 41.0 33.0 41.0 22-23 38.07513759436233 40.0 37.0 41.0 33.0 41.0 24-25 37.958675092620226 40.0 37.0 41.0 33.0 41.0 26-27 37.7448170723887 40.0 37.0 41.0 32.5 41.0 28-29 37.64705090200511 39.5 37.0 41.0 32.5 41.0 30-31 37.62259471716311 39.0 37.0 41.0 32.5 41.0 32-33 37.43313431278916 39.0 36.0 41.0 32.0 41.0 34-35 37.27047071508027 39.0 36.0 41.0 32.0 41.0 36-37 37.0232061431597 39.0 35.5 41.0 31.0 41.0 38-39 36.839783474312156 39.0 35.0 41.0 31.0 41.0 40-41 36.5763713906329 39.0 35.0 41.0 30.5 41.0 42-43 36.299773590261644 39.0 35.0 40.0 30.0 41.0 44-45 35.975879411163675 39.0 35.0 40.0 29.5 41.0 46-47 35.511149910094474 38.0 35.0 40.0 27.5 41.0 48-49 34.97419715602692 38.0 34.0 40.0 26.0 41.0 50-51 34.7587812863117 38.0 34.0 40.0 25.5 41.0 52-53 34.45624170094591 38.0 34.0 40.0 24.0 41.0 54-55 34.149774146025734 38.0 33.0 40.0 24.0 41.0 56-57 33.85511529539716 37.0 33.0 40.0 23.0 41.0 58-59 33.46708594079317 37.0 33.0 40.0 22.5 41.0 60-61 33.08140618573978 36.0 32.0 39.0 21.5 41.0 62-63 32.7891683289713 36.0 32.0 39.0 20.5 40.0 64-65 32.35611866332752 35.0 31.0 39.0 19.5 40.0 66-67 31.942094512385417 35.0 31.0 38.0 18.5 40.0 68-69 31.480258404649266 35.0 31.0 38.0 17.5 40.0 70-71 31.005396896784347 34.5 30.0 37.0 13.5 39.0 72-73 30.604193368280917 34.0 30.0 36.5 11.0 39.0 74-75 30.226712416026185 34.0 29.0 36.0 7.5 39.0 76-77 28.81936170922252 32.5 28.0 34.5 7.0 36.5 78-79 29.62641495398701 34.0 29.0 35.0 7.0 37.0 80-81 29.503391870966972 34.0 29.0 35.0 3.5 37.0 82-83 29.17815616286795 34.0 29.0 35.0 2.0 36.0 84-85 28.741469235037762 34.0 29.0 35.0 2.0 36.0 86-87 28.3455706884891 33.0 28.5 35.0 2.0 35.5 88-89 27.94918093199072 33.0 27.0 35.0 2.0 35.0 90-91 27.508594677832107 33.0 26.5 35.0 2.0 35.0 92-93 27.043390639735765 33.0 25.5 35.0 2.0 35.0 94-95 26.509447989459293 32.5 25.0 34.0 2.0 35.0 96-97 25.835974780280807 32.0 24.0 34.0 2.0 35.0 98-99 24.969757456002725 32.0 19.0 34.0 2.0 35.0 100-101 22.268495615021365 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3018828404670231 1101 2 0.35416330468228097 1101 3 0.10022224365632226 1101 4 0.06504994601601055 1101 5 0.1409089847967877 1101 6 0.04169780975326631 1101 7 0.0403406271567448 1101 8 0.14912408361161766 1101 9 0.18530638486379303 1101 10-11 0.16031644429685343 1101 12-13 0.07976325676360574 1101 14-15 0.2309232503063896 1101 16-17 0.13026163756092046 1101 18-19 0.17414792501402587 1101 20-21 -0.13911084032913124 1101 22-23 -0.1684973730683339 1101 24-25 -0.04853507598658524 1101 26-27 -0.12506999693685117 1101 28-29 -0.1347969707580816 1101 30-31 -0.12647689189412858 1101 32-33 -0.01517668591388599 1101 34-35 -0.043633836569973994 1101 36-37 0.01996710810949054 1101 38-39 0.21786389988325539 1101 40-41 0.27525857584254965 1101 42-43 0.2352599728482545 1101 44-45 0.1962969404646131 1101 46-47 0.37837163762382886 1101 48-49 0.35089510723366857 1101 50-51 0.369907423187378 1101 52-53 0.352149805952763 1101 54-55 0.2953741280973432 1101 56-57 0.3765296659791204 1101 58-59 0.2005691561796965 1101 60-61 0.2397318732305891 1101 62-63 0.02769137429702795 1101 64-65 0.1613288424779853 1101 66-67 0.20092320788563 1101 68-69 0.022785048635192595 1101 70-71 -0.0173090002131282 1101 72-73 0.07018640054030811 1101 74-75 0.12728552403178028 1101 76-77 0.49242215619414864 1101 78-79 0.4602661214421815 1101 80-81 0.2514605274596846 1101 82-83 0.2661529455636469 1101 84-85 0.187299343673331 1101 86-87 0.22530809750765002 1101 88-89 0.013046015566626323 1101 90-91 2.7210929401633166E-4 1101 92-93 0.05983567900532094 1101 94-95 -0.09312541727929613 1101 96-97 -0.2326224444653171 1101 98-99 -0.518710027711272 1101 100-101 -0.5493001886853719 1102 1 0.4078949923507338 1102 2 0.4793527142753611 1102 3 0.20110788582637085 1102 4 0.11059460853314818 1102 5 0.08422544875545412 1102 6 0.10117030271199923 1102 7 0.11091808534888514 1102 8 -0.0929090575040945 1102 9 -0.07986174727682283 1102 10-11 0.10012419488441537 1102 12-13 0.07641878667600821 1102 14-15 0.16118979066467176 1102 16-17 0.250092212666722 1102 18-19 0.2232990521468068 1102 20-21 0.15117114949414656 1102 22-23 -0.08610711407256844 1102 24-25 0.14572552194373856 1102 26-27 -0.10236786643655904 1102 28-29 -0.11353991624415016 1102 30-31 0.008538239680639492 1102 32-33 0.11051022188311066 1102 34-35 0.16350211024487038 1102 36-37 0.1984153163742164 1102 38-39 0.19417127641998633 1102 40-41 0.3319919146859789 1102 42-43 0.3492289170924252 1102 44-45 0.19792082970317182 1102 46-47 0.31375011772708206 1102 48-49 0.2501723838045251 1102 50-51 0.220725778592886 1102 52-53 0.07735357358772177 1102 54-55 0.0795144787514559 1102 56-57 0.337118960284009 1102 58-59 0.4655404612886187 1102 60-61 0.4037446863259717 1102 62-63 0.12620052211810417 1102 64-65 0.11763668525371429 1102 66-67 0.15312682293328095 1102 68-69 0.07664492474277651 1102 70-71 -0.17199676843300793 1102 72-73 -0.08115053978745479 1102 74-75 -0.49083188766361374 1102 76-77 0.02299630832793298 1102 78-79 -0.024088381260941816 1102 80-81 -0.14679711621080216 1102 82-83 -0.19814241896107276 1102 84-85 -0.08000924932333575 1102 86-87 -0.014119403387741869 1102 88-89 -0.09500010394738467 1102 90-91 -0.043721513828046454 1102 92-93 0.19391939221747023 1102 94-95 0.024875434666505214 1102 96-97 -0.07182826990967683 1102 98-99 -0.7223701098117203 1102 100-101 -0.5463077820232414 1103 1 0.44699504076584873 1103 2 0.4004708186811392 1103 3 0.18843052986134623 1103 4 0.06122913135945396 1103 5 -0.009705289147888152 1103 6 0.029026367417380072 1103 7 0.06739716717043365 1103 8 0.18557640372109319 1103 9 0.08690497729812563 1103 10-11 0.19764947042338576 1103 12-13 0.1994018698645803 1103 14-15 0.040650674098152706 1103 16-17 0.125227774994066 1103 18-19 0.10049467769859177 1103 20-21 0.15782281696773026 1103 22-23 0.058208951202395554 1103 24-25 -0.0746740451681589 1103 26-27 0.045535759001310794 1103 28-29 0.005983194854309204 1103 30-31 0.03662142824327219 1103 32-33 -0.04354639532419213 1103 34-35 7.143962927713687E-4 1103 36-37 0.022430068745130427 1103 38-39 0.1485979844768366 1103 40-41 0.14255096688886937 1103 42-43 0.2893889717730289 1103 44-45 0.3001114713458719 1103 46-47 0.4206505534631333 1103 48-49 0.45977324388483964 1103 50-51 0.16892701460233894 1103 52-53 0.3523085760084754 1103 54-55 0.4631749463845196 1103 56-57 0.38614481965233693 1103 58-59 0.45580717162381745 1103 60-61 0.3785611360739196 1103 62-63 0.335504341216442 1103 64-65 0.2060696018373136 1103 66-67 0.06834853568923904 1103 68-69 0.34609171083339874 1103 70-71 0.04616844103357565 1103 72-73 0.09851226316301975 1103 74-75 0.31831173435672255 1103 76-77 0.3108933834852827 1103 78-79 0.4082973448234135 1103 80-81 0.2861117779309694 1103 82-83 0.41397289445561114 1103 84-85 0.40355979917721996 1103 86-87 0.490856531451211 1103 88-89 0.5740891222651321 1103 90-91 0.2575152076141549 1103 92-93 0.41606290031988635 1103 94-95 0.49075502342280686 1103 96-97 -0.028881782853598992 1103 98-99 -0.20772738489502274 1103 100-101 -0.4665350482779047 1104 1 0.2650954764168887 1104 2 0.35658298702802327 1104 3 0.09757668528024865 1104 4 0.04215044680540103 1104 5 0.06569285186464668 1104 6 0.07084635074625822 1104 7 0.08714143843791078 1104 8 0.07289911576676644 1104 9 -0.018608760650700162 1104 10-11 -0.037613571584692806 1104 12-13 -0.05073779010743351 1104 14-15 0.2566532219490014 1104 16-17 0.026975726490533702 1104 18-19 0.08499886907050325 1104 20-21 0.030364062269150338 1104 22-23 0.06644354220942716 1104 24-25 0.16487095899962867 1104 26-27 0.15656873951497374 1104 28-29 -0.006304523560999087 1104 30-31 -0.025047728606828912 1104 32-33 0.10550665344671017 1104 34-35 0.1188307166928837 1104 36-37 0.09336365162105409 1104 38-39 0.10174489481450166 1104 40-41 0.3219847778131779 1104 42-43 0.22379062001234473 1104 44-45 0.42803843295511257 1104 46-47 0.5972972986623191 1104 48-49 0.38896516625402455 1104 50-51 0.29283922384065875 1104 52-53 0.3008967206383275 1104 54-55 0.31141630020551503 1104 56-57 0.27013708173496553 1104 58-59 0.3770005723755929 1104 60-61 0.11720700053454891 1104 62-63 0.007251349064453905 1104 64-65 0.12135639233163431 1104 66-67 0.14260769301116483 1104 68-69 0.22173677319283414 1104 70-71 0.17227589113815966 1104 72-73 0.2555314349542215 1104 74-75 0.07183295858332528 1104 76-77 0.20757756007336425 1104 78-79 0.4195148086109022 1104 80-81 0.40949884680157567 1104 82-83 0.4492766706221971 1104 84-85 0.49253861426930357 1104 86-87 0.5158864922897983 1104 88-89 0.5197871862130405 1104 90-91 0.26645706314157636 1104 92-93 0.08249496549453639 1104 94-95 0.14067119598465894 1104 96-97 -0.09735810514100152 1104 98-99 0.10957205048865148 1104 100-101 0.12363512299429047 1105 1 0.47208932265865755 1105 2 0.4343105438343393 1105 3 0.2562372629085239 1105 4 0.11084702087872955 1105 5 -0.09645966667758898 1105 6 0.07556918984221994 1105 7 0.17990427000194842 1105 8 0.05472065457998809 1105 9 0.2050981917992658 1105 10-11 0.14884790386658153 1105 12-13 0.13469170910017425 1105 14-15 0.1900215162098604 1105 16-17 0.10916602188412128 1105 18-19 0.021532696423840036 1105 20-21 0.09836953830070172 1105 22-23 0.16304377212362198 1105 24-25 0.010690887387731607 1105 26-27 0.2687711533942334 1105 28-29 0.15566647413326962 1105 30-31 0.11258701994191966 1105 32-33 0.154113210763704 1105 34-35 0.028464656365677854 1105 36-37 0.2061100785361063 1105 38-39 0.23811009644367687 1105 40-41 0.004588889452712408 1105 42-43 0.07321916820456664 1105 44-45 -0.09644105463956265 1105 46-47 -0.11926989482388706 1105 48-49 0.12417533845521689 1105 50-51 0.1573563490779577 1105 52-53 0.11685875915225807 1105 54-55 0.01864661810694912 1105 56-57 0.0050463066574337745 1105 58-59 0.10864681809950127 1105 60-61 0.22344963254654715 1105 62-63 0.24669822884745685 1105 64-65 0.12173482025485782 1105 66-67 0.13136695497048123 1105 68-69 0.018467859306618095 1105 70-71 0.24113640179971796 1105 72-73 0.040443883657637514 1105 74-75 0.03907018563348075 1105 76-77 -0.012150037762896915 1105 78-79 0.16847935214916276 1105 80-81 0.094660002878733 1105 82-83 0.24188278247127215 1105 84-85 0.23554465812591374 1105 86-87 -0.02315715843698385 1105 88-89 0.021511909022830622 1105 90-91 -0.19307880764928242 1105 92-93 0.09561373910964832 1105 94-95 0.15760154106842705 1105 96-97 0.1421862207480835 1105 98-99 0.42492028843592067 1105 100-101 0.4418019712428638 1106 1 0.21346362026893217 1106 2 0.3262124680119065 1106 3 0.031055642708224696 1106 4 -0.005086368895682369 1106 5 -0.1726459807520797 1106 6 -0.11984280870225916 1106 7 0.01473594332146888 1106 8 -0.011300779620746937 1106 9 -0.006894135627256048 1106 10-11 -0.1051675802497698 1106 12-13 -0.01560460656514806 1106 14-15 0.05105554908343635 1106 16-17 -0.035256779702329766 1106 18-19 -0.13304949940232547 1106 20-21 -0.10498245837866449 1106 22-23 -0.1148728480675274 1106 24-25 -0.13487795457664475 1106 26-27 -0.09824039203687818 1106 28-29 -0.11495470992255719 1106 30-31 -0.20719024503065953 1106 32-33 -0.07894852273322073 1106 34-35 -0.281555494196688 1106 36-37 -0.1349823031981927 1106 38-39 0.04941479964345774 1106 40-41 -0.06370428294431463 1106 42-43 -0.194306518276818 1106 44-45 -0.12240707479377733 1106 46-47 0.14051533346077605 1106 48-49 0.1511621436768138 1106 50-51 0.1722482455164993 1106 52-53 0.33790421128735915 1106 54-55 0.06826463512339132 1106 56-57 0.06325436944234042 1106 58-59 0.20223767757036626 1106 60-61 0.04417934072910157 1106 62-63 -0.365878867167595 1106 64-65 -0.08049102146998166 1106 66-67 0.12315404654216877 1106 68-69 0.1103952002552333 1106 70-71 0.17015321161095542 1106 72-73 0.2321622219493733 1106 74-75 0.3182640442833211 1106 76-77 0.19481927742883798 1106 78-79 0.4353584195182343 1106 80-81 0.14915314857900341 1106 82-83 0.26514964211035874 1106 84-85 0.2891923304799633 1106 86-87 0.34901271153520597 1106 88-89 0.4355408237998475 1106 90-91 0.17872649622741577 1106 92-93 0.4059753703909301 1106 94-95 0.46904544223193056 1106 96-97 0.43606032178980314 1106 98-99 0.346533320839562 1106 100-101 0.3052595115211325 1107 1 0.5691145844384913 1107 2 0.49170751280379577 1107 3 0.35627207796805394 1107 4 0.045168992464446944 1107 5 0.18435012203125467 1107 6 0.18493704001571132 1107 7 0.134997631132606 1107 8 0.12127829333920914 1107 9 0.21340455885122367 1107 10-11 0.26627417494114525 1107 12-13 0.27895464556235794 1107 14-15 0.40702398746349644 1107 16-17 0.3786424485320694 1107 18-19 0.3525397818698437 1107 20-21 0.3185419583440492 1107 22-23 0.2345856415076426 1107 24-25 0.23334721487089638 1107 26-27 0.29883385470085244 1107 28-29 0.2372240000342316 1107 30-31 0.25567794059846705 1107 32-33 0.3146150811326649 1107 34-35 0.2920946837731364 1107 36-37 0.391238302605565 1107 38-39 0.3573386795391542 1107 40-41 0.5511918219887875 1107 42-43 0.4290677220116166 1107 44-45 0.4016184674844183 1107 46-47 0.5148604229370619 1107 48-49 0.4724203243000531 1107 50-51 0.575533777291561 1107 52-53 0.3483566753786036 1107 54-55 0.5092267163415087 1107 56-57 0.7565105569264077 1107 58-59 0.5879339096466936 1107 60-61 0.7357805931207153 1107 62-63 0.3079987252185532 1107 64-65 0.38747940083598564 1107 66-67 0.7482918618282213 1107 68-69 0.687422812449924 1107 70-71 0.573704009020183 1107 72-73 0.6367157311334779 1107 74-75 0.821611151467529 1107 76-77 0.7045162527096132 1107 78-79 0.6248989686910384 1107 80-81 0.48272103661924604 1107 82-83 0.45992218162763976 1107 84-85 0.4373280236409478 1107 86-87 0.892155038256476 1107 88-89 0.751347047062147 1107 90-91 0.7247207164309053 1107 92-93 0.696319547752676 1107 94-95 0.6355121039848477 1107 96-97 0.6815949529669219 1107 98-99 0.8847023967784722 1107 100-101 0.8571801729116579 1108 1 0.3676655880388964 1108 2 0.43722078843391543 1108 3 0.18206328827342944 1108 4 0.10221586291200424 1108 5 0.06098663282772776 1108 6 0.021653573784078617 1108 7 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-1.2276136260740245 2316 56-57 -1.1653247790018284 2316 58-59 -1.3748858709140315 2316 60-61 -1.173596698581207 2316 62-63 -1.307570039941936 2316 64-65 -1.36002088121964 2316 66-67 -1.1707370206674028 2316 68-69 -1.5463307837592453 2316 70-71 -1.3305081391154268 2316 72-73 -1.2454555330398165 2316 74-75 -1.3746995610049701 2316 76-77 -1.5259901781585548 2316 78-79 -1.0435129171328477 2316 80-81 -1.0501349175749226 2316 82-83 -0.9089536856933904 2316 84-85 -1.2099138249515953 2316 86-87 -1.1038538747011621 2316 88-89 -0.740640810443356 2316 90-91 -1.1182042708765927 2316 92-93 -1.2942756601223167 2316 94-95 -1.4240545175529178 2316 96-97 -1.2225695982825826 2316 98-99 -1.283832888379763 2316 100-101 -1.5352402874031128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 19.0 5 44.0 6 102.0 7 252.0 8 563.0 9 1156.0 10 1791.0 11 2772.0 12 3725.0 13 5180.0 14 6720.0 15 8994.0 16 11636.0 17 15036.0 18 12650.0 19 8444.0 20 7658.0 21 7948.0 22 8174.0 23 9306.0 24 10862.0 25 12851.0 26 15761.0 27 19631.0 28 24028.0 29 29490.0 30 36183.0 31 44385.0 32 55695.0 33 70513.0 34 92222.0 35 124881.0 36 172891.0 37 215069.0 38 126184.0 39 6735.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.727711357924576 19.297068641020697 14.889526142929146 22.08569385812558 2 16.0881767434224 20.794510801899055 38.83912079793129 24.278191656747254 3 19.465963464978437 25.15391087534245 29.785345632522393 25.594780027156723 4 13.488883967242357 16.866577677976668 36.056153516606706 33.58838483817427 5 14.02782753528888 37.91730401407023 32.830181580952164 15.22468686968871 6 32.26612378490733 35.994103770560066 16.737049200511986 15.002723244020618 7 29.530823958733233 33.32002349599551 19.48423382790637 17.66491871736489 8 28.015554554230178 33.57216938663311 18.914019875833755 19.498256183302967 9 27.81394664792384 16.255726737773127 19.665177159059233 36.2651494552438 10-11 25.8076017574153 26.4728100390019 25.98407860744033 21.735509596142478 12-13 26.87827046068607 23.89253223434454 27.037603885221795 22.191593419747598 14-15 23.584414829331187 25.14393227199448 25.85975672058008 25.411896178094253 16-17 22.657304749388874 28.57131864007093 26.31329191038347 22.458084700156725 18-19 22.52135630377552 26.24104257922417 29.531379722819075 21.706221394181235 20-21 23.53391571709385 25.309282713770383 29.564982074470674 21.591819494665092 22-23 22.92542806048659 25.35157798073359 29.77730295066857 21.94569100811125 24-25 23.086055366073253 25.532828129528944 29.70264056342508 21.678475940972724 26-27 22.68329740817281 25.9563203629396 29.585972856482044 21.774409372405543 28-29 22.242661990268143 26.039086460646345 29.732523570809903 21.98572797827561 30-31 22.49027626605196 25.634276450736643 29.76287684011351 22.11257044309788 32-33 22.34861861304663 25.90022513644568 29.582142310517284 22.169013939990407 34-35 22.922696371361123 25.634364122483984 29.360689560235198 22.082249945919692 36-37 23.30163124800134 25.385452667472684 29.1286529506624 22.184263133863574 38-39 22.746565158320358 25.64150248084962 29.393343986921565 22.218588373908457 40-41 22.779244187587587 25.61869240707536 29.375651440801075 22.22641196453597 42-43 22.910174888920054 25.78833412574353 28.855859445814534 22.445631539521884 44-45 22.850540707504923 26.020268782611854 28.605789065288906 22.523401444594317 46-47 23.36660440542797 26.42542823661893 27.932096060441143 22.27587129751196 48-49 23.61752624879803 26.50755557493814 27.376821874162253 22.498096302101573 50-51 23.32964818363161 26.48117794826939 27.408450535629242 22.78072333246976 52-53 23.679873454250078 26.65562241484347 27.16988553167384 22.494618599232602 54-55 23.218317775070837 26.651211731612594 27.218671796268495 22.91179869704807 56-57 23.399934501867893 26.938330002847373 26.987282588527222 22.67445290675751 58-59 23.650051943277088 26.386543484859498 27.07352004411972 22.889884527743696 60-61 23.49676055651561 26.312173415614144 27.65348343852154 22.537582589348702 62-63 23.090357345627922 26.69382614286985 27.681716818638886 22.53409969286335 64-65 23.04137593078954 26.61187904247231 27.475641471885385 22.871103554852763 66-67 23.22818571296038 26.746397757359997 27.155285376966255 22.87013115271337 68-69 23.118628474493377 26.485923326655602 27.492584811882086 22.902863386968935 70-71 23.13814028214439 26.633052397389406 27.35705313955964 22.87175418090656 72-73 23.27900095805219 26.674079707719265 27.00599926211598 23.04092007211256 74-75 23.21054652130158 26.982132612151055 26.854221370240626 22.953099496306734 76-77 23.290796793791767 26.891988198814325 26.94046865294023 22.87674635445368 78-79 23.240949653096223 26.763561143527564 26.88926485986815 23.106224343508067 80-81 23.18851610481094 27.370118388864682 26.759552203002674 22.681813303321704 82-83 23.46288120918002 27.26427296341033 26.41186091009182 22.860984917317825 84-85 23.246468395129295 27.037385415241484 26.505549664796824 23.2105965248324 86-87 23.20048605582459 26.873781974375177 26.532884447154252 23.392847522645976 88-89 23.110586140285527 26.907653364728944 26.469990775471906 23.51176971951362 90-91 23.345197312237886 26.868940165947457 26.63707727893379 23.148785242880866 92-93 23.145856535466397 26.6611800970054 26.72060605931053 23.47235730821767 94-95 22.95090378071373 26.780957006657413 26.682021482775852 23.586117729853004 96-97 23.2327123622697 26.694173532693977 26.599381736866185 23.47373236817014 98-99 23.000151786267832 26.797331127087865 26.257912660898793 23.944604425745517 100-101 22.842847302599765 26.96660536713658 26.36890288093539 23.821644449328264 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 400.0 1 308.5 2 258.5 3 287.0 4 243.0 5 251.0 6 416.0 7 903.5 8 1421.5 9 1622.0 10 1491.0 11 1289.5 12 1312.0 13 1458.0 14 1639.5 15 1819.5 16 1983.0 17 2042.0 18 2104.0 19 2217.5 20 2409.5 21 2676.5 22 2998.5 23 3411.0 24 3958.5 25 4646.5 26 5432.0 27 6528.0 28 7829.5 29 9481.5 30 11576.5 31 13919.5 32 16874.5 33 19122.5 34 20823.5 35 22508.5 36 24829.5 37 27146.0 38 28188.5 39 28855.5 40 29621.5 41 31796.0 42 34097.5 43 36403.5 44 38554.5 45 40465.0 46 40891.0 47 41391.0 48 45707.0 49 46142.5 50 42846.5 51 40673.5 52 39689.0 53 40552.5 54 40966.0 55 39075.0 56 35720.5 57 32949.0 58 31215.0 59 28801.5 60 24893.0 61 20774.0 62 17766.5 63 14543.0 64 10973.0 65 8706.0 66 6619.5 67 5310.5 68 3998.0 69 2466.0 70 1856.0 71 1503.0 72 1269.0 73 1171.0 74 978.0 75 796.0 76 611.5 77 399.0 78 261.5 79 192.0 80 143.5 81 112.5 82 79.0 83 45.5 84 25.0 85 14.0 86 7.0 87 2.5 88 1.5 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0997288726282768 2 0.011713796886182081 3 0.004531614853778469 4 0.010602268714500568 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0040186018514639255 10-11 2.1375541763105985E-4 12-13 8.550216705242395E-5 14-15 4.275108352621197E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010687770881552994 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.00410410401851635 34-35 3.420086682096958E-4 36-37 0.0023085585104154465 38-39 1.710043341048479E-4 40-41 0.0032490823479921094 42-43 0.011371788217972384 44-45 0.0024368117609940825 46-47 0.0022658074268892344 48-49 0.010816024132131629 50-51 1.710043341048479E-4 52-53 0.0020093009257319628 54-55 0.01192755230381314 56-57 0.005301134357250284 58-59 0.0013680346728387831 60-61 0.005985151693669677 62-63 0.004189606185568773 64-65 0.022059559099525377 66-67 0.003975850767937714 68-69 1.710043341048479E-4 70-71 0.0015390390069436309 72-73 4.275108352621197E-5 74-75 0.0 76-77 0.0014962879234174191 78-79 0.012355063139075261 80-81 0.020221262507898262 82-83 0.014834625983595553 84-85 0.010345762213343298 86-87 0.012654320723758742 88-89 0.03381610706923367 90-91 0.028685977046088232 92-93 0.0032918334315183216 94-95 0.009618993793397694 96-97 0.013338338060178133 98-99 0.013124582642547076 100-101 0.014236110814228587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1169561.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.699725703455975 #Duplication Level Percentage of deduplicated Percentage of total 1 75.84767867132284 38.45456503879936 2 13.018202392322117 13.200385808856113 3 4.49761529283746 6.840835849995841 4 2.111629936298136 4.2823623423008685 5 1.1335378444444093 2.873502889390915 6 0.6838879498124946 2.0803758880435406 7 0.4571109825362591 1.6222781001437914 8 0.3325816594526009 1.3489439126597658 9 0.23573755341726657 1.0756646366633296 >10 1.4658827223368618 14.043919678682204 >50 0.13671788612376123 4.773704007314267 >100 0.07484512380920794 6.688415773655045 >500 0.003555988556239813 1.1677488281953494 >1k 8.466639419618601E-4 1.032790648668582 >5k 1.6933278839237201E-4 0.5145065966309903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5993 0.5124144871451767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4960 0.42409074858002277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3214 0.27480396490649056 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1475 0.1261156964023253 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1334 0.11405989084793354 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 8.550216705242395E-5 10-11 0.0 0.0 0.0 0.0 8.550216705242395E-5 12-13 0.0 0.0 0.0 0.0 8.550216705242395E-5 14-15 0.0 0.0 0.0 0.0 1.710043341048479E-4 16-17 0.0 0.0 0.0 0.0 3.420086682096958E-4 18-19 0.0 0.0 0.0 0.0 3.420086682096958E-4 20-21 0.0 0.0 0.0 0.0 3.420086682096958E-4 22-23 0.0 0.0 0.0 2.1375541763105985E-4 3.847597517359077E-4 24-25 0.0 0.0 0.0 4.275108352621197E-4 4.275108352621197E-4 26-27 0.0 0.0 0.0 8.122705869980275E-4 4.275108352621197E-4 28-29 0.0 0.0 0.0 0.001410785756364995 4.275108352621197E-4 30-31 0.0 0.0 0.0 0.0034200866820969576 4.275108352621197E-4 32-33 0.0 0.0 0.0 0.005814147359564828 4.275108352621197E-4 34-35 0.0 0.0 0.0 0.008122705869980274 4.275108352621197E-4 36-37 0.0 0.0 0.0 0.010345762213343296 4.275108352621197E-4 38-39 0.0 0.0 0.0 0.012739822890811167 4.275108352621197E-4 40-41 0.0 0.0 0.0 0.016202660656434338 4.275108352621197E-4 42-43 0.0 0.0 0.0 0.023513095939416584 4.275108352621197E-4 44-45 0.0 0.0 0.0 0.03454287548917927 4.275108352621197E-4 46-47 0.0 0.0 0.0 0.04728269837999044 4.275108352621197E-4 48-49 0.0 0.0 0.0 0.07126605623819536 4.275108352621197E-4 50-51 0.0 0.0 0.0 0.09695945743744876 4.275108352621197E-4 52-53 0.0 0.0 0.0 0.12222534780144004 4.275108352621197E-4 54-55 0.0 0.0 0.0 0.170021059183745 4.275108352621197E-4 56-57 0.0 0.0 0.0 0.21225912970764244 4.275108352621197E-4 58-59 0.0 0.0 0.0 0.266424752535353 4.275108352621197E-4 60-61 0.0 0.0 0.0 0.3268747846414167 4.275108352621197E-4 62-63 0.0 0.0 0.0 0.40652005325074964 4.275108352621197E-4 64-65 0.0 0.0 0.0 0.4633789943406116 4.275108352621197E-4 66-67 0.0 0.0 0.0 0.5631172722072642 4.275108352621197E-4 68-69 0.0 0.0 0.0 0.7013742763310336 4.275108352621197E-4 70-71 0.0 0.0 0.0 0.7795660081004753 4.275108352621197E-4 72-73 0.0 0.0 0.0 0.8983285181362921 5.985151693669676E-4 74-75 0.0 0.0 0.0 1.0516339036612883 5.985151693669676E-4 76-77 0.0 0.0 0.0 1.1551342768782473 5.985151693669676E-4 78-79 0.0 0.0 0.0 1.2570528600047368 5.985151693669676E-4 80-81 0.0 0.0 0.0 1.4169419123927698 6.412662528931796E-4 82-83 0.0 0.0 0.0 1.6250969380818958 6.840173364193916E-4 84-85 0.0 0.0 0.0 1.8666405600049933 6.840173364193916E-4 86-87 0.0 0.0 0.0 2.2201492696832403 6.840173364193916E-4 88-89 0.0 0.0 0.0 2.622736223249578 6.840173364193916E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5585 0.0 33.282497 1 GTATCAA 8455 0.0 31.869656 1 GTACATG 7045 0.0 25.362385 1 ATCAACG 10590 0.0 25.338905 3 TCAACGC 10615 0.0 25.232328 4 TATCAAC 10815 0.0 24.943485 2 CAACGCA 10785 0.0 24.833538 5 AACGCAG 10970 0.0 24.458029 6 TACATGG 7400 0.0 24.067717 2 GAGTACT 6050 0.0 23.311068 12-13 ACATGGG 7335 0.0 23.180258 3 AGTACTT 6355 0.0 22.60325 12-13 TGCACCG 380 0.0 22.494097 5 ACGCAGA 12240 0.0 21.959106 7 GTACTTT 6385 0.0 21.902086 14-15 CGCAGAG 12410 0.0 21.696562 8 TAGTACT 200 5.397651E-7 21.370304 4 TACTTTT 6840 0.0 20.757553 14-15 GCAGAGT 12910 0.0 20.561995 9 ATGGGAT 1545 0.0 20.285938 5 >>END_MODULE