FastQCFastQC Report
Thu 26 May 2016
SRR936605_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936605_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222912
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.45416186937408415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41690.3409076041448608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25760.2106447561230898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68100.037.823251
GGTATCA40750.035.8028341
ATCAACG86000.029.9507353
TCAACGC86550.029.7604054
TATCAAC86650.029.7260592
CTAGACG650.005830225529.245113
CAACGCA88050.029.1430845
AACGCAG89900.028.5915416
GTATTAG3650.026.0401671
ACGCAGA99800.025.566977
CGCAGAG100500.025.3416168
GTACATG73450.024.9101051
TACATGG75550.024.21772
GCAGAGT106150.023.5432159
GTATAGG3050.023.3721161
ACATGGG76150.023.153183
GAGTACT48750.023.15237812-13
CGCGGAA2550.022.34973382-83
AGTACTT50050.021.74392912-13
GTACTTT51950.021.5890314-15
Produced by FastQC (version 0.11.5)