##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936605_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1222912 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3852165977601 31.0 31.0 34.0 30.0 34.0 2 31.42885914930919 31.0 31.0 34.0 28.0 34.0 3 31.461559785168514 31.0 31.0 34.0 28.0 34.0 4 35.11872481421394 37.0 35.0 37.0 32.0 37.0 5 35.08475262324681 37.0 35.0 37.0 32.0 37.0 6 35.196735333368224 37.0 35.0 37.0 32.0 37.0 7 35.06644141197404 37.0 35.0 37.0 32.0 37.0 8 35.015867045216666 37.0 35.0 37.0 32.0 37.0 9 36.50254311021562 39.0 37.0 39.0 32.0 39.0 10-11 36.3927641563743 39.0 37.0 39.0 32.0 39.0 12-13 36.28185756620264 39.0 35.0 39.0 32.0 39.0 14-15 37.32219979851371 40.0 37.0 41.0 32.0 41.0 16-17 37.20337276925895 39.0 36.5 41.0 31.0 41.0 18-19 37.16420519219699 39.0 36.0 41.0 31.0 41.0 20-21 37.084523252695206 39.0 36.0 41.0 31.0 41.0 22-23 36.933116201329284 39.0 36.0 41.0 31.0 41.0 24-25 37.05140312630836 39.0 36.0 41.0 31.0 41.0 26-27 37.01688633360372 39.0 36.0 41.0 31.0 41.0 28-29 36.907861726763656 39.0 36.0 41.0 30.0 41.0 30-31 36.69804082386958 39.0 36.0 41.0 30.0 41.0 32-33 36.504366626543856 39.0 35.0 41.0 30.0 41.0 34-35 36.269047977287 39.0 35.0 40.0 30.0 41.0 36-37 36.07673569316516 38.5 35.0 40.0 29.5 41.0 38-39 35.859808800633246 38.0 35.0 40.0 28.5 41.0 40-41 35.64008039826251 38.0 35.0 40.0 27.5 41.0 42-43 35.388019743039564 38.0 34.5 40.0 27.0 41.0 44-45 35.084064102731844 38.0 34.0 40.0 26.0 41.0 46-47 34.73884833904647 38.0 34.0 40.0 24.5 41.0 48-49 34.6069631338968 38.0 34.0 40.0 24.0 41.0 50-51 33.658672087607286 37.5 32.5 39.5 23.0 40.5 52-53 33.78531897634498 37.5 33.0 39.5 23.0 40.5 54-55 34.18548268395436 38.0 33.5 40.0 23.0 41.0 56-57 34.07580267427255 38.0 33.5 40.0 23.0 41.0 58-59 33.80713493693741 37.5 33.0 40.0 22.0 41.0 60-61 33.47147955045007 37.0 33.0 40.0 21.5 41.0 62-63 33.135302049534225 36.5 32.5 39.5 20.0 41.0 64-65 32.7754830273969 36.0 32.0 39.0 19.5 41.0 66-67 32.389777841741676 35.5 31.5 39.0 18.0 40.5 68-69 31.98220272595248 35.0 31.0 38.5 16.0 40.0 70-71 31.53806610778208 35.0 31.0 38.0 13.0 40.0 72-73 31.158676176208917 35.0 31.0 37.0 9.5 39.5 74-75 30.73093076198451 35.0 30.0 36.5 7.5 39.0 76-77 30.28604306769416 34.5 30.0 36.0 7.0 39.0 78-79 29.85253272516747 34.0 29.0 36.0 2.0 37.5 80-81 29.402738300057567 34.0 29.0 35.0 2.0 37.0 82-83 28.94297831732782 34.0 29.0 35.0 2.0 36.5 84-85 28.62270425018317 34.0 28.5 35.0 2.0 36.0 86-87 28.310785649335358 34.0 28.0 35.0 2.0 36.0 88-89 27.916186937408416 34.0 27.0 35.0 2.0 35.5 90-91 27.506100602496335 33.0 26.5 35.0 2.0 35.0 92-93 27.135644265490896 33.0 25.5 35.0 2.0 35.0 94-95 26.678720954574 33.0 25.0 35.0 2.0 35.0 96-97 26.08011941987649 33.0 24.5 35.0 2.0 35.0 98-99 25.352993919431654 32.5 21.0 35.0 2.0 35.0 100-101 22.922153433771193 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25966088515509256 1101 2 0.3318845791262639 1101 3 0.38877076356807194 1101 4 0.32108145742644467 1101 5 0.33237468547191895 1101 6 0.13727864594755346 1101 7 0.3424091185354001 1101 8 0.3860402527966471 1101 9 0.6412550021535637 1101 10-11 0.4158294616051279 1101 12-13 0.4785123092143664 1101 14-15 0.6065017305408773 1101 16-17 0.6151242480349595 1101 18-19 0.8158804089585558 1101 20-21 0.5674487276340869 1101 22-23 0.6168405209859102 1101 24-25 0.4602635706584195 1101 26-27 0.4778487015541302 1101 28-29 0.5217084970682038 1101 30-31 0.45968722434630394 1101 32-33 0.3641933032278999 1101 34-35 0.32366392195386595 1101 36-37 0.29315161253182254 1101 38-39 0.3363014464759786 1101 40-41 0.3527126708430046 1101 42-43 0.24228814124194997 1101 44-45 0.15447206337955777 1101 46-47 0.33135205394427203 1101 48-49 0.3714717129220304 1101 50-51 0.23798604150051972 1101 52-53 0.3078150671082085 1101 54-55 0.4594971129527181 1101 56-57 0.22330155720531764 1101 58-59 0.25545545474589204 1101 60-61 0.1595962894659806 1101 62-63 0.05920730529231122 1101 64-65 0.01745303241495577 1101 66-67 0.04166586712853615 1101 68-69 0.16953831021129773 1101 70-71 0.17557796109242219 1101 72-73 0.17918891623732947 1101 74-75 -0.0687778462465829 1101 76-77 -0.07765541219604444 1101 78-79 -0.08581998524591583 1101 80-81 0.0406087613194579 1101 82-83 0.06613415241309539 1101 84-85 0.07943922321454977 1101 86-87 -0.2164634144143811 1101 88-89 -0.08877340790630939 1101 90-91 -0.29780746846869377 1101 92-93 -0.28625134988708467 1101 94-95 -0.11191739235972875 1101 96-97 -0.1924835161332119 1101 98-99 -0.21103106151327822 1101 100-101 0.1582476791951315 1102 1 0.19983733029552653 1102 2 0.33662148015472226 1102 3 0.2582113111202169 1102 4 0.29418483802466966 1102 5 0.4481492901427515 1102 6 0.2716365202576938 1102 7 0.26878812313933764 1102 8 0.1818750085916534 1102 9 0.3136820972495613 1102 10-11 0.27891958739450473 1102 12-13 0.24838588404598028 1102 14-15 0.3045815109450416 1102 16-17 0.162486485789465 1102 18-19 0.345272083722314 1102 20-21 0.3477604298749668 1102 22-23 0.25150523237247313 1102 24-25 0.1714151633778087 1102 26-27 0.16105904666291337 1102 28-29 0.1939351805602243 1102 30-31 0.13641003851222422 1102 32-33 0.051829289411941204 1102 34-35 -0.10443668743562284 1102 36-37 -0.04033412576379902 1102 38-39 -0.12494530720157115 1102 40-41 -0.11486888631369396 1102 42-43 0.009918764687895987 1102 44-45 -0.14085945196713112 1102 46-47 -0.27788401848586375 1102 48-49 -0.13799711339850518 1102 50-51 -0.027225821207856882 1102 52-53 0.008124115623019179 1102 54-55 0.07423925232586015 1102 56-57 0.038552465622750276 1102 58-59 0.15578385334659828 1102 60-61 -0.13465645859175623 1102 62-63 0.016016669628164948 1102 64-65 0.09186876348193351 1102 66-67 0.05380196457556963 1102 68-69 -0.037166494553247276 1102 70-71 -0.33021600748580227 1102 72-73 -0.2099012289021367 1102 74-75 -0.45119082960136225 1102 76-77 -0.19043020069269545 1102 78-79 -0.24693142653706346 1102 80-81 7.884959744934861E-4 1102 82-83 -0.014444581013211888 1102 84-85 -0.0654171784158315 1102 86-87 -0.22013357285011992 1102 88-89 -0.1516229015560242 1102 90-91 -0.05192647978987708 1102 92-93 -0.09856450062010325 1102 94-95 0.011468568600090379 1102 96-97 -0.2396684241719278 1102 98-99 -0.23114920760919233 1102 100-101 -0.3112178852537859 1103 1 0.21621986104890922 1103 2 0.33421733636300743 1103 3 0.38489254066320555 1103 4 0.3850340586663492 1103 5 0.48733113665717553 1103 6 0.28361408647106856 1103 7 0.16132001879994817 1103 8 0.2172252861935462 1103 9 0.4668438819681313 1103 10-11 0.48794979290592977 1103 12-13 0.4935766798192702 1103 14-15 0.3590299807853583 1103 16-17 0.6539329347839029 1103 18-19 0.549311519126455 1103 20-21 0.5642512655574876 1103 22-23 0.5400368967569307 1103 24-25 0.64748301754409 1103 26-27 0.44199991753044543 1103 28-29 0.2409588398284086 1103 30-31 0.1974061618162537 1103 32-33 0.5349830781806659 1103 34-35 0.3707920855738891 1103 36-37 0.41499982148550174 1103 38-39 0.4960564005753696 1103 40-41 0.5561352893490081 1103 42-43 0.4217598215634908 1103 44-45 0.35883861983846543 1103 46-47 0.4850861313782673 1103 48-49 0.4637886487339671 1103 50-51 0.6942391439808517 1103 52-53 0.6786730721304082 1103 54-55 0.6501354826656254 1103 56-57 0.6868893221567944 1103 58-59 0.36607803943082473 1103 60-61 0.45618556992572223 1103 62-63 0.6358982393163259 1103 64-65 0.5798213913571999 1103 66-67 0.5510542910867287 1103 68-69 0.5802883678162907 1103 70-71 0.7324908542237552 1103 72-73 0.7638884141569733 1103 74-75 0.5093103063311943 1103 76-77 0.4844613886116278 1103 78-79 0.5329234335761193 1103 80-81 0.5895338087543358 1103 82-83 0.27543877965489827 1103 84-85 0.3406780763334396 1103 86-87 0.5913059942686125 1103 88-89 0.47185910191593905 1103 90-91 0.7045934628423574 1103 92-93 0.5154715643572096 1103 94-95 0.36006180320859116 1103 96-97 0.5133733507879157 1103 98-99 0.5657992163989647 1103 100-101 0.9156266478151771 1104 1 0.12544125624793523 1104 2 0.19728637573772545 1104 3 0.2326968445045381 1104 4 0.2813422185708063 1104 5 0.30790601505604087 1104 6 0.20602435740850922 1104 7 0.3780259688136809 1104 8 0.29052028044696243 1104 9 0.46792719169478403 1104 10-11 0.3616322117211084 1104 12-13 0.3369076326214966 1104 14-15 0.4556314135656052 1104 16-17 0.30650832412603535 1104 18-19 0.4003186915841681 1104 20-21 0.5752698547856951 1104 22-23 0.3908477288763166 1104 24-25 0.41044583450386085 1104 26-27 -0.14367475523820872 1104 28-29 0.31739298758451895 1104 30-31 0.42074077556362255 1104 32-33 0.19696431568402062 1104 34-35 -0.022619863347678404 1104 36-37 0.10860224345434943 1104 38-39 0.33368706621648414 1104 40-41 0.4252141766050386 1104 42-43 0.5216153089627227 1104 44-45 0.6186346275747354 1104 46-47 0.706571478888435 1104 48-49 0.5100019693792817 1104 50-51 0.3842640463530955 1104 52-53 0.6390126293760687 1104 54-55 0.7339321639180127 1104 56-57 0.6147528122965298 1104 58-59 0.774142478177076 1104 60-61 0.7466079201486053 1104 62-63 0.8941986990124704 1104 64-65 0.5363148170881402 1104 66-67 0.7432775940721044 1104 68-69 0.7537539107744884 1104 70-71 0.8883227428894571 1104 72-73 0.9084573959283375 1104 74-75 0.5985579148753146 1104 76-77 0.8769996612261366 1104 78-79 0.7157813788202354 1104 80-81 0.7580593676825629 1104 82-83 0.74825977624484 1104 84-85 0.835607606195353 1104 86-87 0.7909370577854027 1104 88-89 0.9276863456429041 1104 90-91 0.975863844421017 1104 92-93 0.9856449458634522 1104 94-95 0.8417958871884714 1104 96-97 0.9803718249866549 1104 98-99 1.1672740150087861 1104 100-101 1.58522802956222 1105 1 0.3621594226245506 1105 2 0.3020250585303934 1105 3 0.3896530614042888 1105 4 0.48180400486788244 1105 5 0.46385331340796654 1105 6 0.45376133328210244 1105 7 0.46464480835911814 1105 8 0.39317956854903 1105 9 0.4908412239050577 1105 10-11 0.408455631151611 1105 12-13 0.3557395616069883 1105 14-15 0.25284400219810976 1105 16-17 0.30588857478021936 1105 18-19 0.35110652446469004 1105 20-21 0.41210153754080636 1105 22-23 0.14765610321326506 1105 24-25 0.24469835836524823 1105 26-27 0.4039937769365949 1105 28-29 0.08689944174184916 1105 30-31 -0.06628257041510466 1105 32-33 0.04700531930235741 1105 34-35 -0.0089274718664214 1105 36-37 0.2357045918244296 1105 38-39 0.11532088825451581 1105 40-41 -0.19941491280162893 1105 42-43 0.049461773887408356 1105 44-45 -0.22757102487317127 1105 46-47 0.04606683181169302 1105 48-49 0.17604210493119155 1105 50-51 0.0891214622696026 1105 52-53 -0.05553232436183464 1105 54-55 0.1731586457866925 1105 56-57 0.36436103472185977 1105 58-59 0.2206932704963549 1105 60-61 -0.01566760329962591 1105 62-63 0.0463137762411705 1105 64-65 0.22555694919282132 1105 66-67 0.1621665942515662 1105 68-69 0.12939296207804674 1105 70-71 0.14014234366236877 1105 72-73 0.15549480625248435 1105 74-75 0.22600665513564877 1105 76-77 0.048792301859158727 1105 78-79 0.14681652716360816 1105 80-81 -0.11853017462106763 1105 82-83 -0.053201329788638674 1105 84-85 0.05709548456589175 1105 86-87 0.27549601326778017 1105 88-89 0.2103687334933113 1105 90-91 0.32064428204335016 1105 92-93 0.42834473677629603 1105 94-95 0.4306114981945548 1105 96-97 0.6032637381967092 1105 98-99 0.5623730914361893 1105 100-101 0.9236122157290829 1106 1 0.08514376341738839 1106 2 0.02070403172112023 1106 3 0.2944869337793996 1106 4 0.2895763951306449 1106 5 0.13885553401625828 1106 6 0.1909336193939879 1106 7 0.3260457596502917 1106 8 0.43557054539026296 1106 9 0.5826429122640207 1106 10-11 0.4290498136803862 1106 12-13 0.46624813315498415 1106 14-15 0.5214688099631033 1106 16-17 0.5838704163001935 1106 18-19 0.6199492566314646 1106 20-21 0.32011392766423796 1106 22-23 0.3128774151312257 1106 24-25 0.16747909985684117 1106 26-27 -0.13750024832086893 1106 28-29 0.30738276802149045 1106 30-31 0.32606125115598417 1106 32-33 0.07847591061115367 1106 34-35 0.014514967354223529 1106 36-37 -0.014712316496677147 1106 38-39 0.12768858789591064 1106 40-41 -0.14479883420428763 1106 42-43 -0.18853627371894532 1106 44-45 0.049479249698521244 1106 46-47 -0.020739515470538095 1106 48-49 0.03569944330138952 1106 50-51 -0.1532174391301666 1106 52-53 -0.1343134107008268 1106 54-55 0.21930963495883304 1106 56-57 0.050484366435455286 1106 58-59 0.16181732499618562 1106 60-61 0.3554350114448681 1106 62-63 0.4951270135048915 1106 64-65 0.2424821207892478 1106 66-67 0.36720667840826593 1106 68-69 0.314017818609333 1106 70-71 0.43569964667700134 1106 72-73 0.6572632377367036 1106 74-75 0.3535877857126408 1106 76-77 0.43939076885569506 1106 78-79 0.553981385123052 1106 80-81 0.515646380283691 1106 82-83 0.7408089494506775 1106 84-85 0.47516091674851424 1106 86-87 0.6161915339089887 1106 88-89 0.7980712224904138 1106 90-91 0.6099832287261115 1106 92-93 0.6245927360517705 1106 94-95 0.5274115145660971 1106 96-97 0.6945864718264687 1106 98-99 0.8164548742624405 1106 100-101 1.2929102596779671 1107 1 -0.06181157251432623 1107 2 -2.1050100443531505E-4 1107 3 -0.03154087256336524 1107 4 -0.026487976051839723 1107 5 0.09066991102749 1107 6 0.08537329336122212 1107 7 0.14725554936401863 1107 8 0.26386412171533635 1107 9 0.20487221165867453 1107 10-11 0.15842907455571265 1107 12-13 0.07698066514736013 1107 14-15 0.048651600925417426 1107 16-17 -0.08865897670164458 1107 18-19 0.19588764753049048 1107 20-21 0.15604663367567895 1107 22-23 0.17333822834794432 1107 24-25 0.07355121459037406 1107 26-27 -0.2753163585779248 1107 28-29 -0.07836984214057452 1107 30-31 0.07767630985765095 1107 32-33 -0.08765748780570704 1107 34-35 -0.15741741323956404 1107 36-37 -0.03212563312258254 1107 38-39 -0.10697994992223414 1107 40-41 -0.25263559558435844 1107 42-43 -0.07928523533404785 1107 44-45 -0.12888608221012277 1107 46-47 0.014843862632517357 1107 48-49 0.020682857418670153 1107 50-51 -0.09701245379132217 1107 52-53 0.11183998899588232 1107 54-55 0.24433727809670813 1107 56-57 0.11591712478159621 1107 58-59 0.06136570879652936 1107 60-61 0.05055885617500877 1107 62-63 0.1181443097505337 1107 64-65 0.1539395007505604 1107 66-67 0.1493296416335923 1107 68-69 -0.03899608952268707 1107 70-71 0.1466461804581769 1107 72-73 -0.0926076617404945 1107 74-75 -0.08355030496875315 1107 76-77 0.13598519379657859 1107 78-79 -0.10431718656134947 1107 80-81 0.06203823769916639 1107 82-83 0.12352143452536524 1107 84-85 0.2777194634015494 1107 86-87 0.05088601584756702 1107 88-89 0.11806243224540225 1107 90-91 0.10275310338574783 1107 92-93 0.20576062824327934 1107 94-95 0.1460986726233351 1107 96-97 0.04617717381087516 1107 98-99 0.2737857252940792 1107 100-101 0.8159075149586883 1108 1 0.06900036643714813 1108 2 0.1807444125086164 1108 3 0.05760296076651272 1108 4 0.1992725404063549 1108 5 0.2669367093873092 1108 6 -0.1486871151177951 1108 7 -0.03382541660067062 1108 8 0.1931912894145924 1108 9 0.013810078736071318 1108 10-11 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-2.3960181888652095 2316 76-77 -2.681262980594454 2316 78-79 -3.446602957418545 2316 80-81 -2.9532207418455236 2316 82-83 -2.940224022077235 2316 84-85 -2.811511447257935 2316 86-87 -3.300180120932854 2316 88-89 -3.3531874675759212 2316 90-91 -3.3805759870861998 2316 92-93 -3.982390238479013 2316 94-95 -4.1912064503960735 2316 96-97 -5.01768731862505 2316 98-99 -6.339370209290429 2316 100-101 -6.36993099230839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2640.0 3 1085.0 4 1075.0 5 1276.0 6 1332.0 7 1656.0 8 2082.0 9 2626.0 10 3195.0 11 4122.0 12 5247.0 13 6412.0 14 7164.0 15 9065.0 16 11349.0 17 14047.0 18 11594.0 19 8423.0 20 8631.0 21 9138.0 22 10220.0 23 11478.0 24 13046.0 25 14918.0 26 17751.0 27 20569.0 28 24750.0 29 29443.0 30 35463.0 31 43286.0 32 53701.0 33 69224.0 34 91317.0 35 123245.0 36 169857.0 37 210891.0 38 151427.0 39 20163.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65658314812891 18.223133634295632 14.416579483576179 22.703703733999276 2 15.81158692483728 19.764136051573054 39.75253833054977 24.671738693039895 3 19.02973477846952 24.740650834726452 29.555090637257948 26.674523749546076 4 12.772361388475511 16.138749377208406 36.536861490331084 34.552027743985 5 13.970861958795696 37.206514864786364 33.281380011143376 15.541243165274562 6 32.21067667831948 35.15641298558712 17.51701083768507 15.11589949840834 7 29.41771315105456 32.699483828001576 20.001603577614382 17.881199443329486 8 27.603564188608797 33.012920674551815 19.57178688822316 19.811728248616227 9 27.71070932760027 16.11491651961783 19.915852887352 36.2585212654299 10-11 25.50625620752905 25.995454493392 26.55177999617117 21.94650930290778 12-13 26.59197845497744 23.49970814904319 27.3495638188258 22.558749577153574 14-15 23.464166544643653 24.757065132476093 25.971391806761357 25.8073765161189 16-17 22.539918747208265 28.26369197894561 26.403353191127316 22.79303608271881 18-19 22.512896893169014 26.14251685667196 29.248693334358734 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22.5314513817592 25.798576229301418 28.54684374053208 23.123128648407302 46-47 22.949474448567592 26.03188634285873 27.949327258186646 23.06931195038703 48-49 23.033168069487683 26.215785027798834 27.509684003424667 23.241362899288816 50-51 22.883638283057618 26.348171344252403 27.444371258146067 23.323819114543916 52-53 23.138540369343946 26.244264521768855 27.378276204113106 23.238918904774092 54-55 22.716435352242197 26.219373821637586 27.410871743876196 23.653319082244025 56-57 22.922283420100374 26.54556220421953 27.09172225659463 23.440432119085465 58-59 23.175743311099698 26.053916838134995 27.214108599150126 23.55623125161518 60-61 22.975172209623455 26.125076976045627 27.71194889035912 23.187801923971794 62-63 22.779480108894674 26.352208355515426 27.70038917824429 23.167922357345606 64-65 22.71170072405076 26.344874506454474 27.537683634506667 23.4057411349881 66-67 22.68494515146113 26.486152522607544 27.235852806050264 23.593049519881063 68-69 22.58288371621329 26.32437697665917 27.60321974111541 23.489519566012135 70-71 22.748343490803077 26.266623869679083 27.450233168619448 23.534799470898392 72-73 22.76281853136617 26.424263090839805 27.21804628098633 23.594872096807695 74-75 22.442502640325696 26.8450489651109 27.13195822418011 23.580490170383296 76-77 22.726936323345953 26.5133279332759 27.21322280797807 23.546512935400074 78-79 22.754320607540777 26.48692808453168 27.115878189268106 23.642873118659438 80-81 22.651871800544093 26.91196561240433 26.98867690601599 23.447485681035584 82-83 22.834139869032505 26.81035064369522 26.863956134271326 23.491553353000945 84-85 22.62625791738076 26.576350560294365 26.782614456220244 24.014777066104635 86-87 22.74253139382362 26.459007304236327 26.969371600160937 23.829089701779118 88-89 22.61526345875016 26.45468009018432 26.87583464245614 24.05422180860938 90-91 22.597821989289034 26.447407264451584 27.149818580882997 23.804952165376385 92-93 22.602719839369584 26.277385993501994 27.204773111580483 23.915121055547946 94-95 22.27810181489866 26.241363856430656 27.266537887808646 24.213996440862044 96-97 22.448907825546495 26.27405359873731 27.089524775308927 24.187513800407263 98-99 22.372578872832214 26.335901119577702 26.84986731673085 24.44165269085923 100-101 22.289397111166192 26.207123346839207 27.02843759688686 24.47504194510774 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 205.0 2 187.0 3 251.0 4 228.0 5 217.5 6 390.0 7 764.5 8 1231.5 9 1507.0 10 1381.0 11 1239.5 12 1330.0 13 1432.5 14 1505.5 15 1617.0 16 1715.5 17 1788.5 18 1886.0 19 1959.5 20 2136.5 21 2395.0 22 2752.0 23 3231.5 24 3790.0 25 4614.5 26 5613.0 27 6718.5 28 7957.5 29 9597.0 30 11762.5 31 14333.0 32 17181.5 33 19578.0 34 21727.5 35 23329.5 36 25667.5 37 28140.5 38 29293.0 39 30109.0 40 31114.5 41 33646.5 42 35722.5 43 37990.0 44 40609.5 45 42717.0 46 43038.5 47 43506.5 48 47839.0 49 48104.0 50 44776.5 51 42703.5 52 41768.5 53 43106.0 54 44020.0 55 42139.5 56 38681.5 57 35267.5 58 32952.5 59 29863.0 60 25591.0 61 22012.5 62 19020.5 63 15467.0 64 11807.5 65 9360.0 66 7105.0 67 5561.0 68 4099.5 69 2637.5 70 1984.5 71 1619.0 72 1375.0 73 1203.5 74 957.5 75 766.0 76 609.0 77 380.0 78 245.5 79 195.0 80 131.0 81 93.0 82 74.0 83 42.0 84 32.5 85 23.5 86 16.0 87 8.5 88 4.0 89 7.0 90 6.0 91 4.0 92 3.0 93 1.5 94 2.0 95 1.5 96 0.0 97 1.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.031809320703370315 2 0.021097184425371573 3 0.019298199706929035 4 0.048490815365292025 5 0.05405131358593259 6 0.049308535691856815 7 0.05274296106342893 8 0.04333917730793385 9 0.04325740527527737 10-11 0.04955385178982625 12-13 0.04427955568348336 14-15 0.03802399518526272 16-17 0.04530170609168934 18-19 0.05082131829600168 20-21 0.011979602784174167 22-23 0.019747945886539667 24-25 0.006051130416579443 26-27 0.011284540506594096 28-29 0.006173788465564161 30-31 0.009976187984090433 32-33 0.00474277789407578 34-35 0.007850115135021981 36-37 0.009608213837136278 38-39 0.011529856604563534 40-41 0.0017989847184425372 42-43 0.002616705045007327 44-45 0.002657591061335566 46-47 4.906321959388738E-4 48-49 0.0015536686204731002 50-51 4.497461796106343E-4 52-53 0.0033526533389156375 54-55 0.006664420661503035 56-57 0.01582288831902868 58-59 0.011448084571907054 60-61 0.010548592212685786 62-63 0.006459990579861839 64-65 4.088601632823948E-5 66-67 0.007359482939083107 68-69 0.01676326669457819 70-71 0.006909736759472472 72-73 0.0027802491103202844 74-75 0.004211259681808666 76-77 0.0069915087921289506 78-79 0.006582648628846556 80-81 0.01189783075151769 82-83 0.007318596922754867 84-85 0.016885924743562906 86-87 0.004906321959388738 88-89 0.006787078710487755 90-91 0.0043339177307933855 92-93 0.01876668149466192 94-95 0.01177517270253297 96-97 0.009976187984090433 98-99 0.02154693060498221 100-101 0.03933234770776638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1222912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.19810773864268 #Duplication Level Percentage of deduplicated Percentage of total 1 76.88484588636075 40.13243469045198 2 12.558390150838619 13.110484042347666 3 4.296188642050594 6.727587528098698 4 1.9424616899245923 4.055712982754793 5 1.084902647607108 2.8314932642867263 6 0.6602731714378204 2.0679006083787987 7 0.44778637365634233 1.6361520963206908 8 0.29948237727411464 1.2505930715823257 9 0.22549211085424375 1.0593235346925398 >10 1.3960735268268276 13.766720250914064 >50 0.12866179863349025 4.686141143346463 >100 0.07214105728863365 6.591484390812566 >500 0.0023575480336013037 0.8040710752428855 >1k 7.858493445337679E-4 0.8242528408290246 >5k 1.5716986890675357E-4 0.45564847994071894 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 0.45416186937408415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4169 0.3409076041448608 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2576 0.2106447561230898 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.177203265647896E-5 7 0.0 0.0 0.0 0.0 8.177203265647896E-5 8 0.0 0.0 0.0 0.0 8.177203265647896E-5 9 0.0 0.0 0.0 0.0 8.177203265647896E-5 10-11 0.0 0.0 0.0 0.0 8.177203265647896E-5 12-13 0.0 0.0 0.0 0.0 8.177203265647896E-5 14-15 0.0 0.0 0.0 0.0 8.177203265647896E-5 16-17 0.0 0.0 0.0 0.0 8.177203265647896E-5 18-19 0.0 0.0 0.0 4.088601632823948E-5 8.177203265647896E-5 20-21 0.0 0.0 0.0 1.2265804898471845E-4 8.177203265647896E-5 22-23 0.0 0.0 0.0 2.862021142976764E-4 8.177203265647896E-5 24-25 0.0 0.0 0.0 4.906321959388738E-4 1.6354406531295793E-4 26-27 0.0 0.0 0.0 5.724042285953527E-4 1.6354406531295793E-4 28-29 0.0 0.0 0.0 0.0010630364245342266 2.044300816411974E-4 30-31 0.0 0.0 0.0 0.002862021142976764 2.453160979694369E-4 32-33 0.0 0.0 0.0 0.005683156269625287 2.453160979694369E-4 34-35 0.0 0.0 0.0 0.007686571069709022 2.453160979694369E-4 36-37 0.0 0.0 0.0 0.010589478229014026 2.453160979694369E-4 38-39 0.0 0.0 0.0 0.013206183274021352 2.453160979694369E-4 40-41 0.0 0.0 0.0 0.017172126857860584 2.453160979694369E-4 42-43 0.0 0.0 0.0 0.024040977601004813 2.862021142976764E-4 44-45 0.0 0.0 0.0 0.03597969436885075 4.088601632823948E-4 46-47 0.0 0.0 0.0 0.048449929348963784 6.132902449235922E-4 48-49 0.0 0.0 0.0 0.07171407263973205 6.541762612518317E-4 50-51 0.0 0.0 0.0 0.0995165637429349 6.541762612518317E-4 52-53 0.0 0.0 0.0 0.12805500314004606 7.359482939083106E-4 54-55 0.0 0.0 0.0 0.17752708289721583 8.177203265647896E-4 56-57 0.0 0.0 0.0 0.22385093939711115 8.177203265647896E-4 58-59 0.0 0.0 0.0 0.2778613669667155 8.586063428930291E-4 60-61 0.0 0.0 0.0 0.34229772870002095 8.994923592212686E-4 62-63 0.0 0.0 0.0 0.429548487544484 8.994923592212686E-4 64-65 0.0 0.0 0.0 0.48985536162863724 8.994923592212686E-4 66-67 0.0 0.0 0.0 0.5845064894285117 8.994923592212686E-4 68-69 0.0 0.0 0.0 0.7140742751727025 8.994923592212686E-4 70-71 0.0 0.0 0.0 0.7907355557881516 9.812643918777476E-4 72-73 0.0 0.0 0.0 0.9102044954992674 9.812643918777476E-4 74-75 0.0 0.0 0.0 1.0603379474565626 9.812643918777476E-4 76-77 0.0 0.0 0.0 1.1658647555997488 9.812643918777476E-4 78-79 0.0 0.0 0.0 1.2693881489428511 9.812643918777476E-4 80-81 0.0 0.0 0.0 1.4339952506803435 0.0010221504082059871 82-83 4.088601632823948E-5 0.0 0.0 1.6443129186728072 0.0010630364245342264 84-85 8.177203265647896E-5 0.0 0.0 1.8821468756541764 0.0010630364245342264 86-87 8.177203265647896E-5 0.0 0.0 2.236751295268997 0.0010630364245342264 88-89 8.177203265647896E-5 0.0 0.0 2.6354308404856606 0.0010630364245342264 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6810 0.0 37.82325 1 GGTATCA 4075 0.0 35.802834 1 ATCAACG 8600 0.0 29.950735 3 TCAACGC 8655 0.0 29.760405 4 TATCAAC 8665 0.0 29.726059 2 CTAGACG 65 0.0058302255 29.24511 3 CAACGCA 8805 0.0 29.143084 5 AACGCAG 8990 0.0 28.591541 6 GTATTAG 365 0.0 26.040167 1 ACGCAGA 9980 0.0 25.56697 7 CGCAGAG 10050 0.0 25.341616 8 GTACATG 7345 0.0 24.910105 1 TACATGG 7555 0.0 24.2177 2 GCAGAGT 10615 0.0 23.543215 9 GTATAGG 305 0.0 23.372116 1 ACATGGG 7615 0.0 23.15318 3 GAGTACT 4875 0.0 23.152378 12-13 CGCGGAA 255 0.0 22.349733 82-83 AGTACTT 5005 0.0 21.743929 12-13 GTACTTT 5195 0.0 21.58903 14-15 >>END_MODULE