FastQCFastQC Report
Thu 26 May 2016
SRR936605_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936605_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222912
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62730.5129559608540926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.4523628846556416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34140.2791697194892192No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14720.12036843207033704No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.11570742620891773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA91600.031.118351
GGTATCA61500.030.6673431
GTACATG71950.026.0811671
ATCAACG113600.024.9980413
TCAACGC113800.024.9958364
TATCAAC114700.024.7583032
CAACGCA115700.024.5443175
TACATGG75450.024.420562
AACGCAG117200.024.2707026
ACATGGG74650.023.791673
ATACGCT1801.9560503E-723.743968
GAGTACT63250.023.0936212-13
GTACGTG1652.426832E-623.0338631
GTAAGGT4800.022.7546274
AGTACTT65050.022.60064312-13
TACGCTA1903.292771E-722.4961179
TCTATAC2755.2750693E-1122.4488353
AGGTAAG4250.022.3472542
GTACTTT65950.022.14816714-15
ACGCAGA130500.021.7243587
Produced by FastQC (version 0.11.5)