##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936605_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1222912 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87795115265857 31.0 31.0 34.0 30.0 34.0 2 32.0158744046996 31.0 31.0 34.0 30.0 34.0 3 32.1138691909148 33.0 31.0 34.0 30.0 34.0 4 35.872658866705045 37.0 35.0 37.0 35.0 37.0 5 35.6426627590538 37.0 35.0 37.0 33.0 37.0 6 35.753686283232156 37.0 35.0 37.0 33.0 37.0 7 35.69654971085409 37.0 35.0 37.0 33.0 37.0 8 35.66557119400251 37.0 35.0 37.0 33.0 37.0 9 37.292574608802596 39.0 37.0 39.0 34.0 39.0 10-11 37.159077268029094 39.0 37.0 39.0 33.0 39.0 12-13 37.05968908637743 39.0 37.0 39.0 33.0 39.0 14-15 38.149337401219384 40.0 37.0 41.0 33.0 41.0 16-17 38.068433378689555 40.0 37.0 41.0 33.0 41.0 18-19 38.21377171865187 40.0 37.5 41.0 33.0 41.0 20-21 38.242017414172075 40.0 38.0 41.0 33.0 41.0 22-23 38.14796608423173 40.0 37.0 41.0 33.0 41.0 24-25 38.01095540807515 40.0 37.0 41.0 33.0 41.0 26-27 37.779534013894704 40.0 37.0 41.0 33.0 41.0 28-29 37.689581507091276 40.0 37.0 41.0 32.5 41.0 30-31 37.65387615789198 39.5 37.0 41.0 33.0 41.0 32-33 37.48555701473205 39.5 36.0 41.0 32.0 41.0 34-35 37.3101682704888 39.0 36.0 41.0 32.0 41.0 36-37 37.037822018264606 39.0 35.5 41.0 31.0 41.0 38-39 36.848892643133766 39.0 35.0 41.0 31.0 41.0 40-41 36.588891105819556 39.0 35.0 40.5 30.5 41.0 42-43 36.303643271142974 39.0 35.0 40.0 30.0 41.0 44-45 35.99745934294536 39.0 35.0 40.0 29.5 41.0 46-47 35.550320873456144 38.0 35.0 40.0 27.5 41.0 48-49 35.126445320677206 38.0 34.5 40.0 26.5 41.0 50-51 34.76790930173226 38.0 34.0 40.0 25.5 41.0 52-53 34.45283184726293 38.0 34.0 40.0 24.0 41.0 54-55 34.152630360974456 38.0 33.0 40.0 24.0 41.0 56-57 33.81770846962006 37.0 33.0 40.0 23.0 41.0 58-59 33.45438388044275 37.0 33.0 40.0 22.5 41.0 60-61 33.08744537628219 36.0 32.0 39.0 22.0 40.5 62-63 32.67717955175843 36.0 31.5 39.0 20.0 40.0 64-65 32.255614058902026 35.0 31.0 39.0 19.5 40.0 66-67 31.78471427216349 35.0 31.0 38.0 18.0 40.0 68-69 31.500955915061756 35.0 31.0 38.0 17.5 40.0 70-71 31.071061531819133 34.5 30.0 37.0 13.5 39.0 72-73 30.625333221033074 34.0 30.0 36.5 9.5 39.0 74-75 30.09136348322692 34.0 29.0 36.0 7.5 39.0 76-77 28.688405625261673 32.5 27.0 34.5 7.0 36.5 78-79 29.47521326146117 34.0 29.0 35.0 6.0 37.0 80-81 29.311089023576514 34.0 29.0 35.0 2.0 37.0 82-83 28.985739366364875 34.0 29.0 35.0 2.0 36.0 84-85 28.56178490357442 33.5 28.5 35.0 2.0 36.0 86-87 28.17752626517689 33.0 27.5 35.0 2.0 35.5 88-89 27.788221883504292 33.0 27.0 35.0 2.0 35.0 90-91 27.308553272843834 33.0 26.0 35.0 2.0 35.0 92-93 26.803899626465356 32.5 25.0 34.0 2.0 35.0 94-95 26.20582184163701 32.0 24.5 34.0 2.0 35.0 96-97 25.43527375641093 32.0 21.5 34.0 2.0 35.0 98-99 24.482749371990792 31.5 10.0 34.0 2.0 35.0 100-101 21.370884004736237 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18645080618752985 1101 2 0.23496873043504252 1101 3 0.13637062554877843 1101 4 0.09124567728515132 1101 5 -0.08728274367787492 1101 6 -0.039384305298085565 1101 7 0.0868001815384929 1101 8 0.07481823216008365 1101 9 0.1251994616564076 1101 10-11 0.10382830283625566 1101 12-13 0.08245704001839727 1101 14-15 0.22265485071152114 1101 16-17 0.2596621701079158 1101 18-19 0.12266524419682412 1101 20-21 0.005171633255159236 1101 22-23 -0.050683360084185836 1101 24-25 -0.06137781070501802 1101 26-27 0.04316714043292791 1101 28-29 0.03545213451887008 1101 30-31 0.05380423798850131 1101 32-33 0.1784948416168035 1101 34-35 0.17458226427208245 1101 36-37 0.013187823881239069 1101 38-39 0.1111169376626151 1101 40-41 0.1573555607826762 1101 42-43 0.1789991196854146 1101 44-45 0.25905016483787335 1101 46-47 0.3862824767092192 1101 48-49 0.5664266814318921 1101 50-51 0.44100023806336 1101 52-53 0.4215274152255404 1101 54-55 0.4484267055886946 1101 56-57 0.37102166931256164 1101 58-59 0.4188780536951171 1101 60-61 0.33194504080248777 1101 62-63 0.40174434486792165 1101 64-65 0.2687997020518793 1101 66-67 0.2606414736756264 1101 68-69 0.20342099308373207 1101 70-71 0.12326453032024887 1101 72-73 0.058429313125159865 1101 74-75 0.10862554727299312 1101 76-77 0.05113166732079932 1101 78-79 9.764685927962091E-4 1101 80-81 0.09362442284669825 1101 82-83 0.25133040318695166 1101 84-85 0.27677992172663224 1101 86-87 0.39347795607212177 1101 88-89 0.08022383113167564 1101 90-91 0.2734986897183589 1101 92-93 -0.03222599295471795 1101 94-95 0.09926063889968262 1101 96-97 0.28326134368531797 1101 98-99 0.43950002168955393 1101 100-101 0.8121571169955182 1102 1 0.22502741386868053 1102 2 0.22954821851470086 1102 3 0.1499629551747219 1102 4 0.15563474669805544 1102 5 0.05903265442940864 1102 6 0.0737990464408611 1102 7 0.19309877271402343 1102 8 0.24998681978349424 1102 9 0.3189607175322635 1102 10-11 0.2521652320605483 1102 12-13 0.10344317568184636 1102 14-15 0.2778969963043352 1102 16-17 0.24688337337917687 1102 18-19 0.23721802915793688 1102 20-21 0.1341389882780959 1102 22-23 -0.0240975859909085 1102 24-25 0.13637536789212135 1102 26-27 -0.09432088670142491 1102 28-29 -0.05449010547471289 1102 30-31 0.09897825745746047 1102 32-33 0.20440833843994 1102 34-35 0.1621187357286331 1102 36-37 0.32352906264609516 1102 38-39 0.31077775829974996 1102 40-41 0.42222879698778826 1102 42-43 0.4507554578751467 1102 44-45 0.6016959573690173 1102 46-47 0.3996623700349531 1102 48-49 0.4579000660798158 1102 50-51 0.4706231463536312 1102 52-53 0.4092453628448567 1102 54-55 0.4811037485085663 1102 56-57 0.4505230175989041 1102 58-59 0.5029678602080807 1102 60-61 0.47736204206439226 1102 62-63 0.3789640415967952 1102 64-65 0.3374220174002289 1102 66-67 0.30335720294581137 1102 68-69 0.3422168095951612 1102 70-71 0.2937032658750631 1102 72-73 0.31955968985751326 1102 74-75 0.1913582216898284 1102 76-77 0.1564891532470689 1102 78-79 0.1531226004415167 1102 80-81 0.571827518584012 1102 82-83 0.5178396891549646 1102 84-85 0.41716769744808246 1102 86-87 0.5456065346286358 1102 88-89 0.3924000108995038 1102 90-91 0.4590796967797601 1102 92-93 0.48151920234984047 1102 94-95 0.7826408501196909 1102 96-97 0.8689189218009261 1102 98-99 0.7662447429170136 1102 100-101 1.1816816992064325 1103 1 0.12780766344949512 1103 2 0.19884095066166196 1103 3 0.20023195472204236 1103 4 0.10959556231314593 1103 5 -0.01848180385556475 1103 6 0.12562803182957083 1103 7 0.05864722859316629 1103 8 0.07267613835087872 1103 9 0.03971225220465158 1103 10-11 0.053272537143413956 1103 12-13 0.08403036326514268 1103 14-15 0.09216645276307389 1103 16-17 0.2624795652199339 1103 18-19 0.11896004625262435 1103 20-21 0.13149595314527573 1103 22-23 0.09089263413039816 1103 24-25 0.0979413612725537 1103 26-27 0.3452778209725693 1103 28-29 0.3280481220756286 1103 30-31 0.23243221164503325 1103 32-33 0.17601453720486404 1103 34-35 0.20770058562759175 1103 36-37 0.31725259457137156 1103 38-39 0.3762377933578307 1103 40-41 0.3760133350489383 1103 42-43 0.293039507294921 1103 44-45 0.23247627464040477 1103 46-47 0.18648428316819832 1103 48-49 0.21936240825851883 1103 50-51 0.1972476850352649 1103 52-53 0.18695344439508688 1103 54-55 0.042382338965040844 1103 56-57 0.19979513232315327 1103 58-59 0.3328013554817275 1103 60-61 0.28645669464953727 1103 62-63 0.1315905066395473 1103 64-65 0.026091178276772098 1103 66-67 0.34856083172502395 1103 68-69 0.3200006199825616 1103 70-71 0.2734627950870774 1103 72-73 0.21457188121314275 1103 74-75 0.2766250847531957 1103 76-77 0.08525370143359368 1103 78-79 0.09661069606574557 1103 80-81 0.23413007009584064 1103 82-83 0.24252432861095485 1103 84-85 0.24636546535118242 1103 86-87 0.082367737772298 1103 88-89 -0.07153585791615313 1103 90-91 -0.014594449226002837 1103 92-93 0.10233885066907078 1103 94-95 0.2064305636898176 1103 96-97 0.4370829135144696 1103 98-99 0.6255306919036983 1103 100-101 0.8694780746578807 1104 1 0.11965457360262732 1104 2 0.16989075876762882 1104 3 0.05433167850097931 1104 4 0.08653955321395301 1104 5 0.08599295288868802 1104 6 0.1066253429600934 1104 7 0.1412693820784412 1104 8 0.043172220864548194 1104 9 0.14245065750550623 1104 10-11 0.1580942891470727 1104 12-13 0.08817444315319278 1104 14-15 0.08961632027536126 1104 16-17 0.1809016061876747 1104 18-19 0.10871459485473167 1104 20-21 -0.05453995528709754 1104 22-23 0.019675485994440578 1104 24-25 0.10307386578990219 1104 26-27 0.16567102726072847 1104 28-29 0.18082144081407847 1104 30-31 0.06727163580708861 1104 32-33 0.01946405818028296 1104 34-35 0.16498731800180622 1104 36-37 0.1351948317022149 1104 38-39 0.10953075850643046 1104 40-41 -0.0048364992764931 1104 42-43 0.185922330464237 1104 44-45 0.2681657531513011 1104 46-47 0.049509776242196324 1104 48-49 0.13980453919750602 1104 50-51 -0.09233857621380537 1104 52-53 0.06250927090540159 1104 54-55 -0.0020478752613612983 1104 56-57 0.07468126256662799 1104 58-59 -0.021086442967053642 1104 60-61 0.09419427639053168 1104 62-63 0.03386114827365816 1104 64-65 0.2690972603962223 1104 66-67 0.02060263984077082 1104 68-69 0.33455272412453496 1104 70-71 0.16038878990613625 1104 72-73 0.020029011942803265 1104 74-75 0.22544931935438584 1104 76-77 0.0014457134224237222 1104 78-79 0.20981854882363038 1104 80-81 0.3408750406509 1104 82-83 0.3180875503526899 1104 84-85 0.3410441379621467 1104 86-87 0.2668498836362083 1104 88-89 0.59340759759435 1104 90-91 0.5290798928963483 1104 92-93 0.5500064344055495 1104 94-95 0.6656201930084258 1104 96-97 0.7095203359523978 1104 98-99 0.8126928451872892 1104 100-101 1.581447867887821 1105 1 0.13897113822975626 1105 2 0.20859379240937415 1105 3 0.13831397495401632 1105 4 0.07263171015034686 1105 5 -0.1581058647881477 1105 6 0.08317607267843385 1105 7 0.09929127571526664 1105 8 0.09807533547018465 1105 9 0.04126040488982596 1105 10-11 0.047654225377577575 1105 12-13 -0.041317174580399296 1105 14-15 0.11206762717440455 1105 16-17 0.10402295185756572 1105 18-19 0.14619430171537573 1105 20-21 0.17625616178501957 1105 22-23 0.11790419228333349 1105 24-25 0.04214593199666439 1105 26-27 0.02094129640305198 1105 28-29 -0.016891642365884252 1105 30-31 -0.018373237384267327 1105 32-33 0.17539309792353208 1105 34-35 0.2860971910053607 1105 36-37 0.29504008216859035 1105 38-39 0.421886515497917 1105 40-41 0.36817487740173505 1105 42-43 0.25241496650652095 1105 44-45 0.40278282317672165 1105 46-47 0.4291025988352928 1105 48-49 0.21083261363420291 1105 50-51 0.43215352146035 1105 52-53 0.5066968775878422 1105 54-55 0.42260093344267347 1105 56-57 0.3377878135467469 1105 58-59 0.647210456616861 1105 60-61 0.44046878987686 1105 62-63 0.4463351167144367 1105 64-65 0.29545121448052214 1105 66-67 0.350677594037748 1105 68-69 0.44291913162329166 1105 70-71 0.21209716966850323 1105 72-73 0.23689850566810122 1105 74-75 -0.22552109961286604 1105 76-77 0.01569525330548771 1105 78-79 0.24923784159989992 1105 80-81 0.4135166544197695 1105 82-83 0.4064529562363468 1105 84-85 0.3062260145715108 1105 86-87 0.2726780315246806 1105 88-89 0.27007320004496904 1105 90-91 0.4674351782940498 1105 92-93 0.7078458462905495 1105 94-95 0.4811670331214408 1105 96-97 0.3334044741252029 1105 98-99 0.5137540355233732 1105 100-101 0.6449476571313077 1106 1 0.01734189098984018 1106 2 0.026501735568771778 1106 3 0.022443940626018843 1106 4 0.06838294628896335 1106 5 -0.10031823505672577 1106 6 0.015581191746576906 1106 7 0.03859203664370625 1106 8 -0.05353996112471293 1106 9 -0.16159259780799573 1106 10-11 -0.06273155959438981 1106 12-13 -0.07974828434463177 1106 14-15 0.08558251918317694 1106 16-17 0.08492550285753708 1106 18-19 -0.02721234652459259 1106 20-21 -0.03684255339715037 1106 22-23 -0.04697227222960265 1106 24-25 -0.14512645565361737 1106 26-27 -0.1155087695185415 1106 28-29 -0.1427132674331517 1106 30-31 -0.11371592513886952 1106 32-33 -0.1087374337858833 1106 34-35 -0.09843204874544398 1106 36-37 -0.14196692102037645 1106 38-39 0.04735349635888042 1106 40-41 0.028181243145496637 1106 42-43 -0.052677454339672636 1106 44-45 -0.23370708307007249 1106 46-47 -0.47409651396638 1106 48-49 -0.41848298618257473 1106 50-51 -0.28640897864849535 1106 52-53 -0.38037147534780047 1106 54-55 -0.43297353356779666 1106 56-57 -0.29236987576663864 1106 58-59 -0.09378715383444813 1106 60-61 -0.20316124284880743 1106 62-63 -0.446980547543518 1106 64-65 -0.1579251551123022 1106 66-67 -0.26172742053118725 1106 68-69 -0.14015948487678287 1106 70-71 -0.05806900149772787 1106 72-73 -0.33807663222731676 1106 74-75 -0.27180445445562285 1106 76-77 -0.5560854742528711 1106 78-79 -0.32010631262700784 1106 80-81 -0.26184282287516325 1106 82-83 0.11956691088716909 1106 84-85 -0.09596598705867265 1106 86-87 -0.010181310792102494 1106 88-89 -0.15994302345794864 1106 90-91 0.11525737114038037 1106 92-93 -0.10564156242503131 1106 94-95 0.25616912310571394 1106 96-97 -0.014958894023784808 1106 98-99 0.2573136183625131 1106 100-101 0.6041875211429968 1107 1 -0.10085515634106557 1107 2 0.08237535049750733 1107 3 -0.05088912483302721 1107 4 -0.073487738888808 1107 5 -0.13031122567964104 1107 6 -0.04518460130267954 1107 7 -0.14510275335029377 1107 8 -0.21762188898616586 1107 9 -0.13651956486094718 1107 10-11 -0.028094670626792606 1107 12-13 -0.0812778961104641 1107 14-15 -0.11292175018724038 1107 16-17 -0.09206399696660839 1107 18-19 -0.10032615192186256 1107 20-21 -0.08055244356373237 1107 22-23 -0.20605908879674217 1107 24-25 -0.003071730921725191 1107 26-27 -0.1794124121623355 1107 28-29 -0.2883917359462629 1107 30-31 -0.2988924567720943 1107 32-33 -0.17045914117688454 1107 34-35 -0.13742487120283187 1107 36-37 4.186780581321159E-4 1107 38-39 -0.19011656000881771 1107 40-41 -0.06160252774063224 1107 42-43 -0.4160474318828875 1107 44-45 -0.17302487671857136 1107 46-47 -0.07449455530930749 1107 48-49 -0.24634424317768833 1107 50-51 -0.24303591559039717 1107 52-53 -0.21187086196529492 1107 54-55 0.01595052952949061 1107 56-57 -0.3438453918090829 1107 58-59 -0.1901613761929255 1107 60-61 -0.4743913959687305 1107 62-63 -0.17317904933257466 1107 64-65 -0.11718643720822541 1107 66-67 -0.06640639056448805 1107 68-69 -0.0872719241152069 1107 70-71 -0.29440034412828453 1107 72-73 -0.30839566553847675 1107 74-75 -0.15457894746633727 1107 76-77 -0.49173363824749217 1107 78-79 -0.30499894712594156 1107 80-81 -0.12458131159790398 1107 82-83 -0.1944494621843198 1107 84-85 -0.13544495173448823 1107 86-87 0.06901423297037113 1107 88-89 -0.05310855835576689 1107 90-91 0.04124544279774511 1107 92-93 0.2057687411112532 1107 94-95 0.09141800559869395 1107 96-97 0.17706579406389267 1107 98-99 0.41313931355828615 1107 100-101 0.4491768582583191 1108 1 -0.011912776701009875 1108 2 -0.04461529974895839 1108 3 -0.044810403638024354 1108 4 -0.008826284692467823 1108 5 -0.26005718682095136 1108 6 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-0.679931795519817 2316 82-83 -0.35491801551001956 2316 84-85 -0.4706062511815041 2316 86-87 -0.5859668862964647 2316 88-89 -0.5565712046755564 2316 90-91 -0.7056266255028945 2316 92-93 -0.7187000762147058 2316 94-95 -0.6930823503302328 2316 96-97 -0.5917159390035565 2316 98-99 -0.7338770120294704 2316 100-101 -0.8762694671880062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 18.0 5 45.0 6 106.0 7 239.0 8 636.0 9 1105.0 10 1779.0 11 2856.0 12 3923.0 13 5331.0 14 7109.0 15 9438.0 16 12094.0 17 15865.0 18 13431.0 19 9166.0 20 8319.0 21 8495.0 22 8959.0 23 10036.0 24 11547.0 25 13838.0 26 16549.0 27 20749.0 28 25479.0 29 31323.0 30 37750.0 31 46946.0 32 58589.0 33 73980.0 34 97878.0 35 132010.0 36 181870.0 37 225124.0 38 124473.0 39 5848.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75756167752058 19.255476924561965 14.863630079668075 22.12333131824938 2 16.34524565952843 20.68641232660398 38.64567310326442 24.322668910603173 3 19.48825304406846 25.017540703427184 29.794009175137177 25.700197077367175 4 13.450458332992616 16.858395139462427 36.05457474384869 33.63657178369627 5 14.091703028201724 37.84140046970163 32.85777133773379 15.209125164362861 6 32.36855726795697 35.900708883490104 16.697644714365502 15.033089134187417 7 29.45853830856186 33.26044719489219 19.533457844881724 17.747556651664222 8 28.096461560602886 33.43895554218129 18.931288596399416 19.53329430081641 9 27.80277785728012 16.227936396308728 19.69604664379725 36.2732391026139 10-11 25.853796552554552 26.485120886282708 25.933688253098868 21.727394308063868 12-13 26.89153623574752 23.87297750472953 27.035374476194523 22.20011178332842 14-15 23.629616515459222 25.090023252514452 25.811313225312244 25.469047006714074 16-17 22.7101650098355 28.585949488353794 26.248286301080288 22.455599200730415 18-19 22.558655079024494 26.18283245237597 29.513857088653968 21.744655379945574 20-21 23.583790166422443 25.33751406478962 29.476241953631988 21.602453815155958 22-23 22.97067172786621 25.341514442940277 29.66444067107777 22.02337315811574 24-25 23.065037695278882 25.52885623786438 29.67666572491503 21.729440341941704 26-27 22.70822991994884 25.870287518512185 29.620850755166444 21.80063180637253 28-29 22.28031518213417 25.96691249979045 29.73675806937654 22.016014248698838 30-31 22.5178050927533 25.608873046693724 29.72042968547164 22.152892175081334 32-33 22.347165651736965 25.82236290680664 29.582945255943 22.247526185513394 34-35 22.949334231819154 25.60471966751663 29.313059066174464 22.13288703448975 36-37 23.367244418655716 25.372140732844194 29.071119229380503 22.189495619119587 38-39 22.77994267232038 25.584991760679426 29.3261339799394 22.30893158706079 40-41 22.82266848530047 25.54632609804922 29.374452120213 22.25655329643731 42-43 22.951754692611416 25.769386294046193 28.83360256896173 22.44525644438066 44-45 22.90782238369704 25.9428756936923 28.59118264085741 22.55811928175325 46-47 23.380882426896797 26.325985159187727 27.94261890578345 22.35051350813203 48-49 23.593509537150155 26.443533496551648 27.375006337384356 22.58795062891385 50-51 23.37829759987963 26.504112774828954 27.299110227598888 22.818479397692528 52-53 23.666863471772913 26.53344432923259 27.213280583022858 22.58641161597163 54-55 23.240372714742545 26.54130809970516 27.172203320806744 23.046115864745552 56-57 23.42179569267303 26.876881297055427 26.944589592057522 22.756733418214022 58-59 23.7103349020153 26.269998650742703 27.060009240368142 22.95965720687385 60-61 23.504818851972047 26.284751198733126 27.66271675225201 22.547713197042818 62-63 23.176616200377893 26.62022792220829 27.615297144859525 22.58785873255429 64-65 23.091256962212174 26.54933116556254 27.405916852959468 22.95349501926582 66-67 23.29235148629216 26.680028801369904 27.110904491402728 22.91671522093521 68-69 23.2091674142354 26.45763636348768 27.459432981723886 22.873763240553036 70-71 23.224892715093155 26.565607337240948 27.298325635859328 22.911174311806572 72-73 23.292824085904737 26.604325247702263 27.01642298834032 23.08642767805268 74-75 23.253546962030804 27.07532750466754 26.71815748519595 22.95296804810571 76-77 23.359902000950225 26.918418898508346 26.86072670089764 22.86095239964379 78-79 23.264371876678958 26.714816205001895 26.857760146934595 23.163051771384552 80-81 23.198230982985315 27.34043927754235 26.715861455350147 22.74546828412219 82-83 23.472985140171097 27.2139476802524 26.404389173611637 22.908678005964866 84-85 23.272695601968746 26.945756428745792 26.436764219417842 23.34478374986762 86-87 23.22790521568857 26.775713411285786 26.546083930066043 23.450297442959602 88-89 23.21051566247324 26.78799179716172 26.45832438831402 23.543168152051017 90-91 23.321567197015174 26.797879805482538 26.603354166700743 23.27719883080154 92-93 23.22369115053149 26.565659787044922 26.70831401429568 23.502335048127915 94-95 23.00617196875377 26.654850177962864 26.62136331205139 23.71761454123198 96-97 23.286096810952102 26.656109694383424 26.57041095733585 23.487382537328628 98-99 23.049049901193648 26.73843364641433 26.250310239265517 23.962206213126507 100-101 22.894831962735438 26.77515325181711 26.313271574650155 24.016743210797305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 441.0 1 321.5 2 252.0 3 305.0 4 266.5 5 255.5 6 431.5 7 879.0 8 1357.5 9 1638.5 10 1549.5 11 1368.0 12 1422.5 13 1532.5 14 1706.5 15 1869.5 16 1974.0 17 2112.0 18 2259.5 19 2402.5 20 2521.0 21 2687.0 22 2984.5 23 3437.0 24 3994.5 25 4722.5 26 5670.0 27 6764.0 28 8159.5 29 9977.0 30 12018.5 31 14246.5 32 17231.5 33 19602.5 34 21458.0 35 23188.5 36 25640.5 37 28206.0 38 29357.5 39 30013.0 40 30810.5 41 33241.5 42 35778.5 43 37924.0 44 40157.5 45 42512.5 46 42826.0 47 43202.0 48 47707.0 49 48101.5 50 44621.5 51 42488.5 52 41812.5 53 42615.0 54 42781.5 55 41084.5 56 37520.0 57 34431.0 58 33158.0 59 30676.0 60 26258.5 61 22015.5 62 18881.0 63 15508.0 64 11746.5 65 9377.5 66 7179.0 67 5650.0 68 4198.5 69 2597.5 70 1935.5 71 1588.5 72 1351.5 73 1228.0 74 1032.5 75 844.0 76 630.5 77 418.0 78 282.5 79 205.0 80 161.0 81 126.5 82 102.0 83 68.0 84 35.5 85 17.5 86 8.5 87 4.0 88 2.5 89 2.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03802399518526272 2 0.004988093992045217 3 0.0034344253715721165 4 1.6354406531295793E-4 5 6.541762612518317E-4 6 2.453160979694369E-4 7 0.0 8 0.0 9 0.0022078448817249323 10-11 5.315182122671133E-4 12-13 8.586063428930291E-4 14-15 0.002739363093992045 16-17 0.0037206274858697925 18-19 0.0 20-21 0.0 22-23 0.0011039224408624661 24-25 0.0011039224408624661 26-27 0.005642270253297048 28-29 0.005192524073686414 30-31 0.0014310105714883817 32-33 2.453160979694369E-4 34-35 0.009322011722838601 36-37 0.012715551078082478 38-39 0.009567327820808038 40-41 0.0013083525225036634 42-43 4.497461796106343E-4 44-45 0.0014310105714883817 46-47 4.088601632823948E-5 48-49 8.177203265647896E-4 50-51 0.0012265804898471844 52-53 0.0035979694368850745 54-55 0.0038023995185262715 56-57 0.0015536686204731002 58-59 0.0013901245551601422 60-61 0.004783663910404019 62-63 0.006746192694159514 64-65 0.012306690914800085 66-67 0.004374803747121624 68-69 0.004824549926732259 70-71 0.0 72-73 0.0018398707347707767 74-75 0.00858606342893029 76-77 0.0035161974042285955 78-79 0.0044974617961063435 80-81 0.007032394808457191 82-83 0.006950622775800713 84-85 0.008463405379945572 86-87 0.005928472367594725 88-89 0.01345149937199079 90-91 0.015863774335356916 92-93 0.0020443008164119742 94-95 0.0036388554532133135 96-97 0.0019216427674272557 98-99 0.005356068138999372 100-101 0.007195938873770149 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1222912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.59749674918757 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2349091713679 37.314631630523415 2 13.27064405784601 13.163814510372857 3 4.627984174258763 6.886092901142714 4 2.129758373149291 4.225227359553078 5 1.1557321417724438 2.8660710572245183 6 0.715612668094015 2.129555818768221 7 0.5011568071711212 1.7399286180152032 8 0.3562025016944004 1.41334019358724 9 0.24759033068577252 1.105187455918609 >10 1.5340640094283582 14.371067890298411 >50 0.14042831558975716 4.831252008342622 >100 0.08130000790685313 7.163955179073836 >500 0.0036279893849593954 1.2185972806976233 >1k 6.59634433628981E-4 0.6057407517851264 >5k 3.298172168144905E-4 0.9655373446966539 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6273 0.5129559608540926 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5532 0.4523628846556416 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3414 0.2791697194892192 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1472 0.12036843207033704 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1415 0.11570742620891773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 4.088601632823948E-5 0.0 20-21 0.0 0.0 0.0 1.2265804898471845E-4 0.0 22-23 0.0 0.0 0.0 2.862021142976764E-4 0.0 24-25 0.0 0.0 0.0 4.906321959388738E-4 0.0 26-27 0.0 0.0 0.0 5.724042285953527E-4 0.0 28-29 0.0 0.0 0.0 0.0011448084571907054 0.0 30-31 0.0 0.0 0.0 0.00314822325727444 0.0 32-33 0.0 0.0 0.0 0.005846700334938245 0.0 34-35 0.0 0.0 0.0 0.007768343102365501 0.0 36-37 0.0 0.0 0.0 0.010630364245342265 0.0 38-39 0.0 0.0 0.0 0.013287955306677831 0.0 40-41 0.0 8.177203265647896E-5 0.0 0.01737655693950178 0.0 42-43 0.0 8.177203265647896E-5 0.0 0.024449837764287212 0.0 44-45 0.0 8.177203265647896E-5 0.0 0.0365520985974461 0.0 46-47 0.0 8.177203265647896E-5 0.0 0.049267649675528574 0.0 48-49 0.0 8.177203265647896E-5 0.0 0.07281799508059451 0.0 50-51 0.0 8.177203265647896E-5 0.0 0.10045694211848441 0.0 52-53 0.0 8.177203265647896E-5 0.0 0.12838209127067196 8.177203265647896E-5 54-55 0.0 8.177203265647896E-5 0.0 0.17830391720745237 2.044300816411974E-4 56-57 0.0 8.177203265647896E-5 0.0 0.22454600167469124 2.453160979694369E-4 58-59 0.0 8.177203265647896E-5 0.0 0.2781884550973414 2.453160979694369E-4 60-61 0.0 8.177203265647896E-5 0.0 0.3422159566673645 4.088601632823948E-4 62-63 0.0 8.177203265647896E-5 0.0 0.42922139941385806 4.906321959388738E-4 64-65 0.0 8.177203265647896E-5 0.0 0.4897327035796525 4.906321959388738E-4 66-67 0.0 8.177203265647896E-5 0.0 0.5845882614611682 4.906321959388738E-4 68-69 0.0 8.177203265647896E-5 0.0 0.7144422493196567 4.906321959388738E-4 70-71 0.0 8.177203265647896E-5 0.0 0.7917985922126858 4.906321959388738E-4 72-73 0.0 8.177203265647896E-5 0.0 0.912207910299351 5.724042285953527E-4 74-75 0.0 8.177203265647896E-5 0.0 1.0636088287628218 5.724042285953527E-4 76-77 0.0 8.177203265647896E-5 0.0 1.1700760152815575 5.724042285953527E-4 78-79 0.0 8.177203265647896E-5 0.0 1.2742126988695834 5.724042285953527E-4 80-81 0.0 8.177203265647896E-5 0.0 1.4384518264601214 6.132902449235922E-4 82-83 0.0 8.177203265647896E-5 0.0 1.6494645567301653 6.541762612518317E-4 84-85 0.0 8.177203265647896E-5 0.0 1.8879526899727863 6.541762612518317E-4 86-87 0.0 8.177203265647896E-5 0.0 2.2433748299141723 6.541762612518317E-4 88-89 0.0 8.177203265647896E-5 0.0 2.6414001988695834 7.359482939083106E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9160 0.0 31.11835 1 GGTATCA 6150 0.0 30.667343 1 GTACATG 7195 0.0 26.081167 1 ATCAACG 11360 0.0 24.998041 3 TCAACGC 11380 0.0 24.995836 4 TATCAAC 11470 0.0 24.758303 2 CAACGCA 11570 0.0 24.544317 5 TACATGG 7545 0.0 24.42056 2 AACGCAG 11720 0.0 24.270702 6 ACATGGG 7465 0.0 23.79167 3 ATACGCT 180 1.9560503E-7 23.74396 8 GAGTACT 6325 0.0 23.09362 12-13 GTACGTG 165 2.426832E-6 23.033863 1 GTAAGGT 480 0.0 22.754627 4 AGTACTT 6505 0.0 22.600643 12-13 TACGCTA 190 3.292771E-7 22.496117 9 TCTATAC 275 5.2750693E-11 22.448835 3 AGGTAAG 425 0.0 22.347254 2 GTACTTT 6595 0.0 22.148167 14-15 ACGCAGA 13050 0.0 21.724358 7 >>END_MODULE