FastQCFastQC Report
Thu 26 May 2016
SRR936604_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936604_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272668
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54970.43192725832660206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41460.32577231453921995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26190.20578815527694574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47200.034.6408421
GTATCAA74200.034.3987271
ATCAACG92900.027.3743743
TATCAAC93650.027.2005542
TCAACGC94400.026.990824
GTACATG78100.026.96031
CAACGCA96000.026.4935385
AACGCAG97950.025.9175666
TACATGG81300.025.3699722
ACATGGG79300.024.9359723
ACGCAGA108750.023.3436857
CGCAGAG110000.023.080238
GAGTACT49350.022.63221512-13
CATGGGG32450.022.5594884
GTACACG2806.548362E-1122.0677131
GCAGAGT115500.021.7341929
AGTACTT53200.021.44104812-13
GTACTTT52550.021.250714-15
CATGGGA47050.021.0148834
CCGTTGC1604.954727E-520.8003039
Produced by FastQC (version 0.11.5)