##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936604_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1272668 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.327585041817663 31.0 31.0 34.0 28.0 34.0 2 31.383436214315125 31.0 31.0 34.0 28.0 34.0 3 31.334901168254408 31.0 31.0 34.0 28.0 34.0 4 35.024776296724674 37.0 35.0 37.0 32.0 37.0 5 35.04932315419261 37.0 35.0 37.0 32.0 37.0 6 35.10155122938583 37.0 35.0 37.0 32.0 37.0 7 34.96640364965568 37.0 35.0 37.0 32.0 37.0 8 34.948058723877715 37.0 35.0 37.0 32.0 37.0 9 36.42843302416655 39.0 37.0 39.0 32.0 39.0 10-11 36.30083218875622 39.0 36.0 39.0 32.0 39.0 12-13 36.20045447830856 39.0 35.0 39.0 32.0 39.0 14-15 37.174043819755035 39.5 36.5 41.0 31.0 41.0 16-17 37.08889592572454 39.0 36.0 41.0 31.0 41.0 18-19 36.979635694462345 39.0 36.0 41.0 31.0 41.0 20-21 36.9712815911141 39.0 36.0 41.0 31.0 41.0 22-23 36.93778660263321 39.0 36.0 41.0 31.0 41.0 24-25 37.03271630935955 39.0 36.0 41.0 31.0 41.0 26-27 36.944402232161096 39.0 36.0 41.0 30.0 41.0 28-29 36.818765773948904 39.0 36.0 41.0 30.0 41.0 30-31 36.59476744916978 39.0 36.0 41.0 30.0 41.0 32-33 36.41876750260083 39.0 35.0 40.5 30.0 41.0 34-35 36.221233267435025 39.0 35.0 40.0 30.0 41.0 36-37 35.998063124082634 38.0 35.0 40.0 29.0 41.0 38-39 35.791519469335284 38.0 35.0 40.0 28.5 41.0 40-41 35.54181373303957 38.0 35.0 40.0 27.0 41.0 42-43 35.23925760685427 38.0 34.0 40.0 26.5 41.0 44-45 35.048094632692894 38.0 34.0 40.0 26.0 41.0 46-47 34.84681236583304 38.0 34.0 40.0 24.5 41.0 48-49 34.576301124880956 38.0 34.0 40.0 24.0 41.0 50-51 33.561389537569895 37.0 32.5 39.5 22.5 40.5 52-53 33.65417257289411 37.5 33.0 39.5 23.0 40.5 54-55 34.067851159925446 38.0 33.0 40.0 23.0 41.0 56-57 33.91061455147768 38.0 33.0 40.0 22.0 41.0 58-59 33.68124915531781 37.0 33.0 40.0 22.0 41.0 60-61 33.38448401311261 37.0 33.0 40.0 20.5 41.0 62-63 33.04411598311579 36.0 32.0 39.5 20.0 41.0 64-65 32.670929496145106 36.0 32.0 39.0 18.5 41.0 66-67 32.30261702187845 35.0 31.5 39.0 18.0 40.5 68-69 31.86844055165998 35.0 31.0 38.5 14.0 40.0 70-71 31.467246760349127 35.0 31.0 37.5 12.5 40.0 72-73 31.070585180109816 35.0 30.5 37.0 8.0 39.5 74-75 30.64884007455204 35.0 30.0 36.5 7.0 39.0 76-77 30.186489720806996 34.0 30.0 36.0 6.0 39.0 78-79 29.741272272108674 34.0 29.0 35.5 2.0 37.5 80-81 29.315733168430416 34.0 29.0 35.0 2.0 37.0 82-83 28.92708860441215 34.0 29.0 35.0 2.0 36.5 84-85 28.567909698365952 34.0 28.5 35.0 2.0 36.0 86-87 28.220678527314274 34.0 27.0 35.0 2.0 36.0 88-89 27.8745151917075 34.0 27.0 35.0 2.0 35.5 90-91 27.530234515207425 33.0 26.5 35.0 2.0 35.0 92-93 27.0990635421021 33.0 25.5 35.0 2.0 35.0 94-95 26.60267642464492 33.0 25.0 35.0 2.0 35.0 96-97 25.996569804536612 33.0 24.0 35.0 2.0 35.0 98-99 25.243419729261678 32.5 19.0 35.0 2.0 35.0 100-101 22.744590890947208 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43627894592156125 1101 2 0.41996569356146907 1101 3 0.435381510301049 1101 4 0.52934762849452 1101 5 0.3805575552857192 1101 6 0.47418403509841056 1101 7 0.4370848175758866 1101 8 0.5138083534239115 1101 9 0.6109407175364154 1101 10-11 0.679508168720524 1101 12-13 0.6714593677000167 1101 14-15 0.8217266894110438 1101 16-17 0.980736234991177 1101 18-19 0.9562938381113284 1101 20-21 0.7486493129644387 1101 22-23 0.6866403048078737 1101 24-25 0.5894754927172698 1101 26-27 0.5808346161376008 1101 28-29 0.5392772153786822 1101 30-31 0.5646340663667502 1101 32-33 0.5346192525008817 1101 34-35 0.46140750566048894 1101 36-37 0.6050806060845559 1101 38-39 0.6360550273158836 1101 40-41 0.7119260268305325 1101 42-43 0.7523117829672898 1101 44-45 0.8353064074689414 1101 46-47 0.793426746097019 1101 48-49 0.9026004496707785 1101 50-51 0.8302964970729434 1101 52-53 0.8145391139940088 1101 54-55 0.907110518285478 1101 56-57 1.065789490088605 1101 58-59 0.8822616883857606 1101 60-61 0.860827292466027 1101 62-63 0.8227962271365072 1101 64-65 0.8132693135296023 1101 66-67 0.8146438834302003 1101 68-69 0.9518551287858514 1101 70-71 0.8110482076821803 1101 72-73 0.9907601562863917 1101 74-75 0.8164203789345841 1101 76-77 0.8786664669305182 1101 78-79 0.8993767649938036 1101 80-81 0.6925092700468269 1101 82-83 0.9109115095436273 1101 84-85 0.959937842624182 1101 86-87 0.7654500692033821 1101 88-89 0.8699993545584981 1101 90-91 0.7434943916046883 1101 92-93 0.7515382804720296 1101 94-95 0.738100967921735 1101 96-97 0.42039919099683587 1101 98-99 0.5744558189084508 1101 100-101 0.9041632845775993 1102 1 0.3271198768708281 1102 2 0.3372078048382612 1102 3 0.41725493956656834 1102 4 0.2875660257753623 1102 5 0.2925510875940347 1102 6 0.30773610289910636 1102 7 0.4474742680055357 1102 8 0.32292994759984595 1102 9 0.5161775037121217 1102 10-11 0.3959982929117345 1102 12-13 0.37789351543488436 1102 14-15 0.4538615255829441 1102 16-17 0.33583674889488435 1102 18-19 0.30755645312381574 1102 20-21 0.4428836855485514 1102 22-23 0.18163675386694678 1102 24-25 0.39096007429587587 1102 26-27 0.3391584933982088 1102 28-29 0.18498228025215013 1102 30-31 0.0569922056212917 1102 32-33 0.08548995370033907 1102 34-35 -0.08267709845545568 1102 36-37 -0.15575854284269752 1102 38-39 -0.2435245727249935 1102 40-41 -0.04100173052096778 1102 42-43 -0.15332346857297807 1102 44-45 0.11077189520574393 1102 46-47 0.32190896125521107 1102 48-49 0.536733378030867 1102 50-51 0.45109688702129347 1102 52-53 0.1982143509636245 1102 54-55 0.30827813580066277 1102 56-57 0.19669944818488005 1102 58-59 0.08692717926730609 1102 60-61 0.17229263868932065 1102 62-63 0.10377799023764567 1102 64-65 0.16628879260264284 1102 66-67 0.1683816746139044 1102 68-69 0.19843495682072643 1102 70-71 0.10459964653393072 1102 72-73 0.4469331591769361 1102 74-75 0.07380725329417359 1102 76-77 0.2603766315316989 1102 78-79 0.05810408679815282 1102 80-81 0.385317107798965 1102 82-83 0.1620555414732685 1102 84-85 0.15703105023760955 1102 86-87 -0.04363418112878392 1102 88-89 0.31206870142122156 1102 90-91 0.5486055190218835 1102 92-93 0.5251220315759646 1102 94-95 0.46865766635905715 1102 96-97 0.27939630569353824 1102 98-99 0.31647144304855956 1102 100-101 0.863987336109485 1103 1 0.3124520491924443 1103 2 0.36761896208256317 1103 3 0.521621333082269 1103 4 0.48173215544741765 1103 5 0.3435347887620068 1103 6 0.2326725311010165 1103 7 0.46338416822864303 1103 8 0.23366462563531343 1103 9 0.35552677835563173 1103 10-11 0.12611418764975468 1103 12-13 0.3415193155212606 1103 14-15 0.5266172662644664 1103 16-17 0.6610951870066728 1103 18-19 0.7479525869761474 1103 20-21 0.699377138307355 1103 22-23 0.5323767653667275 1103 24-25 0.37170768291248635 1103 26-27 0.38256349660416333 1103 28-29 0.32026661689785385 1103 30-31 0.43423416925443803 1103 32-33 0.4847197779629582 1103 34-35 0.5191915360228521 1103 36-37 0.46867254095035804 1103 38-39 0.19279338289781123 1103 40-41 0.592975533652087 1103 42-43 0.7362082863610127 1103 44-45 0.6524503294842745 1103 46-47 0.6741045974243249 1103 48-49 0.6251155284900918 1103 50-51 0.6633971957642757 1103 52-53 0.6649267800709922 1103 54-55 0.8205137382498933 1103 56-57 0.8194498767572966 1103 58-59 0.6292648222604811 1103 60-61 0.7111939610574609 1103 62-63 0.6844414326231458 1103 64-65 0.6844305794680139 1103 66-67 0.6903739397559505 1103 68-69 0.7919416909200407 1103 70-71 0.7521410743114068 1103 72-73 0.7363315241150943 1103 74-75 0.5987932522830022 1103 76-77 0.44701144511938296 1103 78-79 0.45162165866778636 1103 80-81 0.7331281184397334 1103 82-83 0.7098017924322519 1103 84-85 0.7986251848149521 1103 86-87 0.6141689601161957 1103 88-89 0.559853632008771 1103 90-91 0.6868526244563036 1103 92-93 0.6564776764793585 1103 94-95 0.7056698265509169 1103 96-97 0.7659632097055962 1103 98-99 1.173422217682198 1103 100-101 1.457733382058425 1104 1 0.19793469313556145 1104 2 0.2150341640094453 1104 3 0.292644616985573 1104 4 0.40897329308421604 1104 5 0.34712264564853257 1104 6 0.31377928025816715 1104 7 0.4031088804676557 1104 8 0.44961293130754143 1104 9 0.5546989873177353 1104 10-11 0.38207470788220377 1104 12-13 0.5309191956083694 1104 14-15 0.5669850808166288 1104 16-17 0.4882666477384703 1104 18-19 0.5547203409728425 1104 20-21 0.4924214826610154 1104 22-23 0.18103730242618354 1104 24-25 0.4271375804310793 1104 26-27 -0.032279018342741495 1104 28-29 -0.28635061973623976 1104 30-31 -0.05143918084056054 1104 32-33 0.07367641055628837 1104 34-35 0.16070181765477543 1104 36-37 0.18059202940914787 1104 38-39 -0.0602914334181861 1104 40-41 0.12202391431071646 1104 42-43 0.14560012869026195 1104 44-45 0.28235895716782267 1104 46-47 0.29681886003355373 1104 48-49 0.12395138778140335 1104 50-51 0.11475746103206319 1104 52-53 0.07755959231147358 1104 54-55 0.1010144698710107 1104 56-57 0.3108252155438933 1104 58-59 0.31695667880283906 1104 60-61 0.20844078393763255 1104 62-63 0.16225046834814805 1104 64-65 0.21100990903405403 1104 66-67 0.19607266106630306 1104 68-69 0.10549075854557088 1104 70-71 0.11541491952357319 1104 72-73 0.41436595468950443 1104 74-75 0.40955783038512195 1104 76-77 0.4752556415695892 1104 78-79 0.15980557737970003 1104 80-81 0.20264671882430108 1104 82-83 0.2515706328332037 1104 84-85 0.16142566816955295 1104 86-87 0.005824912495203449 1104 88-89 0.1052423224042478 1104 90-91 0.10197681489453103 1104 92-93 0.23788121276607654 1104 94-95 0.3330595755779129 1104 96-97 0.2949790526926783 1104 98-99 0.08214220474336642 1104 100-101 0.774481352061688 1105 1 0.28739934303853687 1105 2 0.2907119705898218 1105 3 0.39354680743765513 1105 4 0.37590791118795863 1105 5 0.3618908138324244 1105 6 0.28398575706043516 1105 7 0.3925493827384088 1105 8 0.40153586835786825 1105 9 0.29984051280803214 1105 10-11 0.4881908958147818 1105 12-13 0.30843527705862783 1105 14-15 0.5929258929335006 1105 16-17 0.5738343643907342 1105 18-19 0.5216849254153644 1105 20-21 0.5020750077789202 1105 22-23 0.5166386221088288 1105 24-25 0.29809932534480765 1105 26-27 0.3205673474386046 1105 28-29 0.43760994107069706 1105 30-31 0.1974004236037672 1105 32-33 0.336600630631402 1105 34-35 0.20190306053050477 1105 36-37 0.430155626417168 1105 38-39 0.16928272454224214 1105 40-41 0.1869407504472136 1105 42-43 0.08498418950738085 1105 44-45 0.08701014025633214 1105 46-47 0.3880393046409196 1105 48-49 0.6481048387108004 1105 50-51 0.6641313682062417 1105 52-53 0.7141394729697979 1105 54-55 0.5240763314498054 1105 56-57 0.6332951691978934 1105 58-59 0.6248437900768735 1105 60-61 0.3743856400555643 1105 62-63 0.5972206038367318 1105 64-65 0.7714360129154159 1105 66-67 0.796410436982093 1105 68-69 0.7469500610572197 1105 70-71 0.7294302925463079 1105 72-73 0.6492255347534517 1105 74-75 0.5650615994522958 1105 76-77 0.6086030852663278 1105 78-79 0.6201665117475983 1105 80-81 0.6383824506284803 1105 82-83 0.43651938457518114 1105 84-85 0.6443364179389874 1105 86-87 0.8528284240846098 1105 88-89 0.864635751338934 1105 90-91 0.7046787312855365 1105 92-93 0.7327299118487609 1105 94-95 0.8120191326579729 1105 96-97 1.0584183737659956 1105 98-99 1.2307505156019474 1105 100-101 1.3436772650022029 1106 1 0.3079061397527809 1106 2 0.21308685110050618 1106 3 0.2551725101968465 1106 4 0.3890347237801066 1106 5 0.4078541002341325 1106 6 0.2877705304762941 1106 7 0.4067277013009729 1106 8 0.5785939199124428 1106 9 0.5278974140247996 1106 10-11 0.5701411234520535 1106 12-13 0.6292782640484731 1106 14-15 0.4764671511762302 1106 16-17 0.2921530889739401 1106 18-19 0.43834052075568053 1106 20-21 0.379058530404933 1106 22-23 0.2338037684933667 1106 24-25 -0.24937560215150967 1106 26-27 -0.08287394482820787 1106 28-29 0.09533583663298373 1106 30-31 0.09666809587090341 1106 32-33 0.09597507368967939 1106 34-35 0.12645953239583463 1106 36-37 0.08280140338124653 1106 38-39 -0.00772186060705593 1106 40-41 0.007090294362576799 1106 42-43 0.21485327782416164 1106 44-45 0.2921234728335733 1106 46-47 0.5995151394459342 1106 48-49 0.4931381336995386 1106 50-51 0.44857507456226386 1106 52-53 0.3868294812327875 1106 54-55 0.4137459738729632 1106 56-57 0.4153004834345424 1106 58-59 0.27781522605490494 1106 60-61 0.29019225368787716 1106 62-63 0.1558246446008198 1106 64-65 -0.0909933061169994 1106 66-67 -0.09707962312710805 1106 68-69 0.14874766641033688 1106 70-71 -0.12881811561299372 1106 72-73 -2.665343964309841E-4 1106 74-75 0.25209032549367905 1106 76-77 0.03374930778378982 1106 78-79 0.23613190440163834 1106 80-81 0.24653445723887302 1106 82-83 0.3730369393782418 1106 84-85 0.42801620277720787 1106 86-87 0.5676807433924651 1106 88-89 0.5080853065956497 1106 90-91 0.8300947161205201 1106 92-93 1.011182950522791 1106 94-95 1.2342514360879235 1106 96-97 1.2314453824016312 1106 98-99 1.3190186901292549 1106 100-101 1.9275701053644774 1107 1 0.1393912536561821 1107 2 0.14996902468423912 1107 3 0.1413390247333659 1107 4 0.2752789217952909 1107 5 0.3421766031958171 1107 6 0.22410503553543037 1107 7 0.2757805454873363 1107 8 0.3338870779400622 1107 9 0.4449780688965248 1107 10-11 0.4414895144330018 1107 12-13 0.3556246158381029 1107 14-15 0.09009477542124955 1107 16-17 0.20464070816952784 1107 18-19 0.5172513983847793 1107 20-21 0.2164339940781943 1107 22-23 0.2878792379928896 1107 24-25 0.05168283524531603 1107 26-27 0.18681978805715005 1107 28-29 0.31344191342309813 1107 30-31 0.12436614018932346 1107 32-33 0.3270096234168207 1107 34-35 0.22122560802069557 1107 36-37 0.2315225391137048 1107 38-39 0.3119728433217759 1107 40-41 0.1680931880721559 1107 42-43 0.26275485285669475 1107 44-45 0.4363350826294621 1107 46-47 0.4254400583637974 1107 48-49 0.3676540685230947 1107 50-51 0.29316150714272027 1107 52-53 0.24891217529583543 1107 54-55 0.3859760723367742 1107 56-57 0.46535583291308313 1107 58-59 0.5035750675806057 1107 60-61 0.5185506075749657 1107 62-63 0.47872379202463833 1107 64-65 0.5012868981905498 1107 66-67 0.39056093541710624 1107 68-69 0.4612080963883898 1107 70-71 0.5249796354502969 1107 72-73 0.4879158664114769 1107 74-75 0.39482400710793897 1107 76-77 0.44734780072429814 1107 78-79 0.501990015527852 1107 80-81 0.4496262596897864 1107 82-83 0.46813316978743913 1107 84-85 0.427989013559678 1107 86-87 0.5223485494168187 1107 88-89 0.7653360882856539 1107 90-91 0.5106952094106099 1107 92-93 0.7315321377593946 1107 94-95 0.5996628672118085 1107 96-97 0.8106281522060641 1107 98-99 0.8986733870665837 1107 100-101 1.0909870131736064 1108 1 -0.01919593735477676 1108 2 0.06865421306027386 1108 3 0.2789331534044308 1108 4 0.33675237749982045 1108 5 0.3262593983635611 1108 6 0.35972030829216806 1108 7 0.4152291097629046 1108 8 0.49767823026449065 1108 9 -0.2516680261270352 1108 10-11 0.20895307135590713 1108 12-13 0.13655442027310727 1108 14-15 0.621020998679576 1108 16-17 0.7575942375930893 1108 18-19 0.5020665953433578 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-8.221468846527635 2316 100-101 -9.135736346248427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3008.0 3 1141.0 4 1245.0 5 1394.0 6 1538.0 7 1889.0 8 2382.0 9 2863.0 10 3625.0 11 4624.0 12 5836.0 13 6698.0 14 7844.0 15 9388.0 16 11832.0 17 14959.0 18 11780.0 19 8804.0 20 9028.0 21 9887.0 22 10714.0 23 12059.0 24 13891.0 25 15763.0 26 18823.0 27 21682.0 28 25529.0 29 31201.0 30 37549.0 31 45966.0 32 56366.0 33 71999.0 34 94267.0 35 127780.0 36 174040.0 37 216724.0 38 157859.0 39 20689.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.666880557626314 18.196930664729067 14.41459931617802 22.7215894614666 2 15.816954934348853 19.748478095979184 39.819611634460074 24.61495533521189 3 19.143267819968045 24.71773246059021 29.435040547347942 26.703959172093803 4 12.75560487718234 16.07451897005126 36.595372115431466 34.57450403733493 5 13.985744118800584 37.228693792506114 33.23401322154778 15.55154886714553 6 32.27373408518191 35.11761127206931 17.465623678513996 15.143030964234791 7 29.396591925147863 32.70392969007421 20.03276117918226 17.866717205595666 8 27.6234736549963 33.041541428606244 19.543584417463173 19.79140049893428 9 27.5947726706199 16.191227340388973 19.90617291328701 36.30782707570411 10-11 25.435464182586937 26.007158961577094 26.614746831835166 21.942630024000803 12-13 26.56786988853428 23.498435706761832 27.296366752087035 22.63732765261685 14-15 23.444957835227235 24.73698111546419 25.88861236707563 25.92944868223294 16-17 22.65236130792226 28.18208082829049 26.280849123538 22.88470874024925 18-19 22.51704592130124 26.10970942437908 29.163294560830956 22.20995009348873 20-21 23.439302031419274 25.238568084590536 29.07856626131541 22.243563622674785 22-23 23.010042959299668 25.162290905933162 29.22921986807687 22.598446266690296 24-25 22.817236701671987 25.556831381869223 29.219029250741524 22.40690266571726 26-27 22.507748466713327 25.894399215309328 29.070808823211564 22.52704349476578 28-29 22.182220371214385 25.823209542818525 29.40428106680916 22.59028901915793 30-31 22.364575488024844 25.49979157753218 29.334725697567016 22.800907236875965 32-33 22.188313701360624 25.75723463635567 29.136102830438144 22.918348831845563 34-35 22.825079458257548 25.693199379029146 28.86465358007141 22.61706758264189 36-37 22.999417339398033 25.410563562063963 28.698411650697526 22.89160744784048 38-39 22.595160046316323 25.478928783740724 28.934814921337292 22.991096248605658 40-41 22.630494357080416 25.550157648145056 29.101631486410827 22.7177165083637 42-43 22.635712517728965 25.679711779103986 28.50813469690838 23.17644100625867 44-45 22.543656456063815 25.819815756554753 28.472558350043688 23.16396943733774 46-47 22.97345716845033 26.07118161959989 27.91656844718935 23.038792764760423 48-49 23.024177706535916 26.28918957144878 27.50530385177502 23.181328870240282 50-51 22.912810704902878 26.334370669716467 27.468146203533493 23.284672421847162 52-53 23.168339470724195 26.320429555809525 27.27822397672283 23.233006996743445 54-55 22.82041117638531 26.17761697790975 27.358720732195536 23.643251113509404 56-57 22.948441716210205 26.59497979755119 27.019380521099023 23.43719796513958 58-59 23.1854898910131 26.075057165083333 27.145242529250453 23.59421041465312 60-61 22.993795389289033 26.15597806698402 27.714320738141225 23.135905805585722 62-63 22.850223628917433 26.305706859105925 27.67529646939757 23.168773042579073 64-65 22.7069967351974 26.30550851952399 27.545544192698003 23.44195055258061 66-67 22.708028852953657 26.595437901717663 27.160474910816713 23.536058334511967 68-69 22.60104115509282 26.324015322659854 27.560003928887145 23.514939593360182 70-71 22.74852953367428 26.275645007368535 27.45920784384907 23.516617615108114 72-73 22.745936022388666 26.38786667552112 27.26543985344571 23.6007574486445 74-75 22.562789566395505 26.783037435819455 27.0546713928539 23.59950160493114 76-77 22.771440326727703 26.581482102950037 27.1349355083743 23.51214206194796 78-79 22.75712802046171 26.466500396290392 27.180255346973166 23.596116236274735 80-81 22.664944621608495 26.949913478630556 26.96708415912388 23.418057740637067 82-83 22.821086544411596 26.782818807767487 26.964138730367647 23.43195591745327 84-85 22.643676986773052 26.51086844634218 26.823685457427104 24.021769109457665 86-87 22.731840858177144 26.471868564123234 26.9407716648162 23.855518912883422 88-89 22.684846687109694 26.462773324008904 26.852749299194397 23.99963068968701 90-91 22.597784580228915 26.409959920020775 27.14394099233801 23.8483145074123 92-93 22.65866950614325 26.217432133497308 27.230724498736773 23.893173861622667 94-95 22.375263704479668 26.141016719522163 27.26400782085895 24.219711755139215 96-97 22.47380188052793 26.303226288098763 27.08773570449071 24.135236126882592 98-99 22.398316827247655 26.301185435871478 26.822539864928057 24.477957871952803 100-101 22.27589166282877 26.237859537660384 27.007783728047357 24.47846507146349 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 204.5 2 193.0 3 263.5 4 249.5 5 246.0 6 384.0 7 773.0 8 1297.0 9 1540.0 10 1406.0 11 1253.5 12 1309.0 13 1481.5 14 1592.5 15 1668.5 16 1738.5 17 1777.0 18 1902.5 19 2047.0 20 2236.0 21 2482.0 22 2792.0 23 3322.0 24 3913.5 25 4615.5 26 5605.0 27 6835.0 28 8203.0 29 10029.0 30 12339.0 31 14823.0 32 17819.5 33 20372.5 34 22517.5 35 24365.0 36 26836.5 37 29357.0 38 30512.5 39 31409.0 40 32408.0 41 34821.5 42 37150.0 43 39467.5 44 42020.0 45 44219.0 46 44837.0 47 45678.5 48 49999.5 49 50128.5 50 46715.0 51 44632.5 52 43770.5 53 44901.5 54 45613.0 55 43774.5 56 40191.0 57 36709.5 58 34409.0 59 31208.5 60 26842.5 61 23102.0 62 19869.5 63 16047.0 64 12202.5 65 9753.5 66 7426.0 67 5761.5 68 4238.5 69 2741.5 70 2124.0 71 1705.5 72 1371.0 73 1210.0 74 1005.0 75 828.5 76 632.5 77 407.5 78 276.5 79 189.0 80 129.5 81 94.0 82 61.5 83 39.5 84 30.5 85 26.0 86 16.0 87 8.0 88 7.5 89 6.0 90 4.0 91 3.0 92 2.0 93 3.0 94 2.0 95 1.0 96 1.5 97 1.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07794648722211919 2 0.03245151131324116 3 0.016107893024732294 4 0.023101075850103875 5 0.02757985586185871 6 0.03347298745627296 7 0.08179666653046984 8 0.05948134155962121 9 0.07598211002398111 10-11 0.06564948596177479 12-13 0.041487646424676346 14-15 0.04054474536957007 16-17 0.05111309469555297 18-19 0.04632001433209604 20-21 0.018268707942684186 22-23 0.014497103722259066 24-25 0.022786775498401784 26-27 0.025104740592204723 28-29 0.006914607737446058 30-31 9.821885990690424E-4 32-33 0.009939748622578709 34-35 0.01135410020523813 36-37 0.004518067555717595 38-39 0.008996847567472427 40-41 0.004360917379866548 42-43 0.0025929779015422716 44-45 3.1430035170209355E-4 46-47 6.286007034041871E-4 48-49 6.286007034041871E-4 50-51 0.0013750640386966592 52-53 3.5358789566485526E-4 54-55 1.9643771981380848E-4 56-57 0.0039287543962761694 58-59 0.002985853341169889 60-61 0.005185955803084543 62-63 0.0018858021102125613 64-65 2.3572526377657016E-4 66-67 6.286007034041871E-4 68-69 0.0033787287807975058 70-71 0.002160814917951893 72-73 0.018740158470237328 74-75 0.014418528634333542 76-77 0.023061788306141116 78-79 0.019054458821939423 80-81 0.011786263188828508 82-83 0.004360917379866548 84-85 0.003064428429095412 86-87 0.0013357764947338976 88-89 0.0020036647421008465 90-91 0.006875320193483296 92-93 0.01045048669409461 94-95 0.01363277775507831 96-97 0.01202198845260508 98-99 0.024279702168986728 100-101 0.021215273739891315 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1272668.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.20045208678048 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95697040877263 40.171886465669196 2 12.528700784607425 13.080076900330177 3 4.283012801101667 6.707256135329251 4 1.9427131020280195 4.056420088030973 5 1.0488941254593809 2.7376373770073963 6 0.6675627120411958 2.0908245218896604 7 0.44102841674802773 1.6115317916154857 8 0.30168966065838654 1.2598669341020137 9 0.23276435649283267 1.0935364174752964 >10 1.3916484523194261 13.731449981416654 >50 0.12943524895074002 4.7125079345298175 >100 0.07193753141261079 6.596262889987596 >500 0.0027317995558372404 0.9043290979912868 >1k 7.588332099547889E-4 0.8109253304458004 >5k 1.517666419909578E-4 0.4354881341794221 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5497 0.43192725832660206 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4146 0.32577231453921995 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2619 0.20578815527694574 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.857508792552339E-5 0.0 0.0 0.0 0.0 3 7.857508792552339E-5 0.0 0.0 0.0 0.0 4 7.857508792552339E-5 0.0 0.0 0.0 0.0 5 7.857508792552339E-5 0.0 0.0 0.0 0.0 6 7.857508792552339E-5 0.0 0.0 0.0 0.0 7 7.857508792552339E-5 0.0 0.0 0.0 0.0 8 7.857508792552339E-5 0.0 0.0 0.0 0.0 9 7.857508792552339E-5 0.0 0.0 0.0 0.0 10-11 7.857508792552339E-5 0.0 0.0 0.0 0.0 12-13 7.857508792552339E-5 0.0 0.0 0.0 0.0 14-15 7.857508792552339E-5 0.0 0.0 0.0 3.9287543962761694E-5 16-17 7.857508792552339E-5 0.0 0.0 0.0 7.857508792552339E-5 18-19 7.857508792552339E-5 0.0 0.0 0.0 7.857508792552339E-5 20-21 7.857508792552339E-5 0.0 0.0 3.9287543962761694E-5 7.857508792552339E-5 22-23 7.857508792552339E-5 0.0 0.0 1.5715017585104678E-4 1.9643771981380848E-4 24-25 7.857508792552339E-5 0.0 0.0 3.1430035170209355E-4 3.1430035170209355E-4 26-27 7.857508792552339E-5 0.0 0.0 6.678882473669488E-4 3.1430035170209355E-4 28-29 7.857508792552339E-5 0.0 0.0 0.0014929266705849442 3.1430035170209355E-4 30-31 7.857508792552339E-5 0.0 0.0 0.0027894156213560803 3.1430035170209355E-4 32-33 7.857508792552339E-5 0.0 0.0 0.004910942995345212 3.9287543962761697E-4 34-35 7.857508792552339E-5 0.0 0.0 0.0067967451055577725 3.9287543962761697E-4 36-37 7.857508792552339E-5 0.0 0.0 0.009232572831248998 3.9287543962761697E-4 38-39 7.857508792552339E-5 0.0 0.0 0.011943413364679554 4.321629835903787E-4 40-41 7.857508792552339E-5 0.0 0.0 0.016068605480769535 5.500256154786637E-4 42-43 7.857508792552339E-5 0.0 0.0 0.023218938481992163 5.500256154786637E-4 44-45 7.857508792552339E-5 0.0 0.0 0.035319502022522764 5.500256154786637E-4 46-47 7.857508792552339E-5 0.0 0.0 0.04632001433209604 5.893131594414254E-4 48-49 7.857508792552339E-5 0.0 0.0 0.06855676421501916 6.286007034041871E-4 50-51 7.857508792552339E-5 0.0 0.0 0.09350435463137283 6.286007034041871E-4 52-53 7.857508792552339E-5 0.0 0.0 0.1217520987405985 6.678882473669488E-4 54-55 7.857508792552339E-5 0.0 0.0 0.16681491166588613 7.071757913297105E-4 56-57 7.857508792552339E-5 0.0 0.0 0.20979548476114745 7.464633352924722E-4 58-59 7.857508792552339E-5 0.0 0.0 0.2605942791049983 7.857508792552339E-4 60-61 7.857508792552339E-5 0.0 0.0 0.3179540932906304 7.857508792552339E-4 62-63 7.857508792552339E-5 0.0 0.0 0.3953112673533081 7.857508792552339E-4 64-65 7.857508792552339E-5 0.0 0.0 0.45278894417082854 7.857508792552339E-4 66-67 7.857508792552339E-5 0.0 0.0 0.5475505002090097 7.857508792552339E-4 68-69 7.857508792552339E-5 0.0 0.0 0.6767279447585702 7.857508792552339E-4 70-71 7.857508792552339E-5 0.0 0.0 0.7501956519689346 7.857508792552339E-4 72-73 7.857508792552339E-5 0.0 0.0 0.86436525472472 7.857508792552339E-4 74-75 7.857508792552339E-5 0.0 0.0 1.012125707568667 7.857508792552339E-4 76-77 7.857508792552339E-5 0.0 0.0 1.117769913284533 7.857508792552339E-4 78-79 7.857508792552339E-5 0.0 0.0 1.21901391407657 7.857508792552339E-4 80-81 7.857508792552339E-5 0.0 0.0 1.3718817476356757 7.857508792552339E-4 82-83 7.857508792552339E-5 0.0 0.0 1.5782985036160255 7.857508792552339E-4 84-85 7.857508792552339E-5 0.0 0.0 1.8158309944148827 7.857508792552339E-4 86-87 7.857508792552339E-5 0.0 0.0 2.163172170589659 7.857508792552339E-4 88-89 7.857508792552339E-5 0.0 0.0 2.5440649093086334 7.857508792552339E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4720 0.0 34.640842 1 GTATCAA 7420 0.0 34.398727 1 ATCAACG 9290 0.0 27.374374 3 TATCAAC 9365 0.0 27.200554 2 TCAACGC 9440 0.0 26.99082 4 GTACATG 7810 0.0 26.9603 1 CAACGCA 9600 0.0 26.493538 5 AACGCAG 9795 0.0 25.917566 6 TACATGG 8130 0.0 25.369972 2 ACATGGG 7930 0.0 24.935972 3 ACGCAGA 10875 0.0 23.343685 7 CGCAGAG 11000 0.0 23.08023 8 GAGTACT 4935 0.0 22.632215 12-13 CATGGGG 3245 0.0 22.559488 4 GTACACG 280 6.548362E-11 22.067713 1 GCAGAGT 11550 0.0 21.734192 9 AGTACTT 5320 0.0 21.441048 12-13 GTACTTT 5255 0.0 21.2507 14-15 CATGGGA 4705 0.0 21.014883 4 CCGTTGC 160 4.954727E-5 20.800303 9 >>END_MODULE