FastQCFastQC Report
Thu 26 May 2016
SRR936604_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936604_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272668
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64400.5060235662403706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55480.43593458781080374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34230.26896252596906656No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16020.12587729085668847No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.12139851084493364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62550.032.806881
GTATCAA91300.030.6445351
GTAAGGT4950.028.7798184
AGGTAAG4850.027.419312
GTACATG75250.026.3231621
TAAGGTG5800.026.1995585
TACATGG78750.024.968362
ATCAACG113550.024.5931053
TCAACGC114100.024.4716744
GGTAAGG5650.024.3765773
CAACGCA115100.024.300325
TATCAAC116850.024.0620842
ACATGGG77300.023.8383083
TAACGCC1000.001656440723.743354
AACGCAG118400.023.6622056
GTACTAC2052.6688213E-823.1715281
GAGTACT65800.022.51737612-13
GTACTTT67750.021.72737714-15
ACGCAGA131050.021.2711097
TAGGACA4050.021.1052024
Produced by FastQC (version 0.11.5)