##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936604_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1272668 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89257921154614 31.0 31.0 34.0 30.0 34.0 2 32.05024876872837 31.0 31.0 34.0 30.0 34.0 3 32.119504851225926 33.0 31.0 34.0 30.0 34.0 4 35.839141865749745 37.0 35.0 37.0 35.0 37.0 5 35.67027221553461 37.0 35.0 37.0 33.0 37.0 6 35.758343888586815 37.0 35.0 37.0 33.0 37.0 7 35.70194504772651 37.0 35.0 37.0 33.0 37.0 8 35.664048282820026 37.0 35.0 37.0 33.0 37.0 9 37.291784660257036 39.0 37.0 39.0 34.0 39.0 10-11 37.17787121228788 39.0 37.0 39.0 33.0 39.0 12-13 37.0708338702631 39.0 37.0 39.0 33.0 39.0 14-15 38.18608859498314 40.0 37.5 41.0 33.0 41.0 16-17 38.08560245091414 40.0 37.0 41.0 33.0 41.0 18-19 38.22821348537089 40.0 37.5 41.0 33.5 41.0 20-21 38.23802397797383 40.0 38.0 41.0 33.0 41.0 22-23 38.1544342279369 40.0 37.0 41.0 33.0 41.0 24-25 38.015869024757436 40.0 37.0 41.0 33.0 41.0 26-27 37.79955220057391 40.0 37.0 41.0 33.0 41.0 28-29 37.70402964480917 40.0 37.0 41.0 32.5 41.0 30-31 37.66456294964594 40.0 37.0 41.0 33.0 41.0 32-33 37.487048468257235 39.5 36.0 41.0 32.0 41.0 34-35 37.30343302416655 39.0 36.0 41.0 32.0 41.0 36-37 37.08579495987956 39.0 36.0 41.0 31.0 41.0 38-39 36.89643331960889 39.0 35.0 41.0 31.0 41.0 40-41 36.67620306317123 39.0 35.0 41.0 30.5 41.0 42-43 36.36851873387246 39.0 35.0 40.0 30.0 41.0 44-45 36.033907507692504 39.0 35.0 40.0 29.5 41.0 46-47 35.576718358597844 38.0 35.0 40.0 28.0 41.0 48-49 35.12809310833619 38.0 34.5 40.0 26.5 41.0 50-51 34.80053988942913 38.0 34.0 40.0 26.0 41.0 52-53 34.4075571948065 38.0 34.0 40.0 24.0 41.0 54-55 34.18732379536533 38.0 33.0 40.0 24.0 41.0 56-57 33.86437979111599 37.0 33.0 40.0 23.0 41.0 58-59 33.49246386331706 37.0 33.0 40.0 22.5 41.0 60-61 33.05759436082309 36.0 32.0 39.0 21.0 41.0 62-63 32.71944254118121 36.0 32.0 39.0 20.5 40.0 64-65 32.28852929436428 35.0 31.0 39.0 19.5 40.0 66-67 31.865362372590496 35.0 31.0 38.0 18.0 40.0 68-69 31.508169059016176 35.0 31.0 38.0 18.0 40.0 70-71 31.14741786546059 35.0 30.0 37.0 14.5 39.0 72-73 30.713773348587377 34.0 30.0 36.5 12.0 39.0 74-75 30.206763272118103 34.0 29.0 36.0 7.5 39.0 76-77 28.710596950657987 32.5 27.0 34.5 7.0 36.5 78-79 29.48899398743427 34.0 29.0 35.0 6.5 37.0 80-81 29.285833775972996 34.0 29.0 35.0 2.0 37.0 82-83 29.01610907165105 34.0 29.0 35.0 2.0 36.0 84-85 28.664843462709833 34.0 29.0 35.0 2.0 36.0 86-87 28.271202701725823 33.0 28.0 35.0 2.0 35.5 88-89 27.8266802496802 33.0 27.0 35.0 2.0 35.0 90-91 27.35637534690901 33.0 26.0 35.0 2.0 35.0 92-93 26.714418057183806 32.5 25.0 34.0 2.0 35.0 94-95 26.182769583269163 32.0 24.5 34.0 2.0 35.0 96-97 25.493876250522526 32.0 22.5 34.0 2.0 35.0 98-99 24.643637225105053 32.0 13.0 34.0 2.0 35.0 100-101 21.563663893489895 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18584078456779096 1101 2 0.2246695897478581 1101 3 0.192467806707036 1101 4 0.1749020460777615 1101 5 0.13944075566926273 1101 6 0.03560736015950283 1101 7 0.13189195278426524 1101 8 0.0992412707669601 1101 9 0.09860413071402263 1101 10-11 0.22901064354263667 1101 12-13 0.19998162240155892 1101 14-15 0.26272773980208086 1101 16-17 0.2778915075153421 1101 18-19 0.19707179631423344 1101 20-21 0.148691410260696 1101 22-23 0.16040084186511194 1101 24-25 0.1868973405633838 1101 26-27 0.1190402835656954 1101 28-29 0.1855560259801905 1101 30-31 0.4001370643504103 1101 32-33 0.32074678537018286 1101 34-35 0.24633397870514528 1101 36-37 0.2028175679866493 1101 38-39 0.18889946818800496 1101 40-41 0.18573758840843624 1101 42-43 0.2726396864338412 1101 44-45 0.3739907357131642 1101 46-47 0.2822718398350901 1101 48-49 0.19856711420600703 1101 50-51 0.10744429866662841 1101 52-53 0.38411382570452446 1101 54-55 0.36263129190526655 1101 56-57 0.3235016040235692 1101 58-59 0.1149933042550586 1101 60-61 0.3137339533143262 1101 62-63 0.17982098421322235 1101 64-65 0.01783500047498876 1101 66-67 -0.17950160537834847 1101 68-69 -0.07526374342079833 1101 70-71 -0.11181190123925688 1101 72-73 -0.001585194497852882 1101 74-75 -0.03376902068476895 1101 76-77 -0.15031314144274077 1101 78-79 -0.01816737051697359 1101 80-81 -0.11789652445209597 1101 82-83 -0.0011883105653502923 1101 84-85 0.04598691272441968 1101 86-87 0.06777037630546445 1101 88-89 -0.014686975750493048 1101 90-91 0.054489195021538706 1101 92-93 -0.1823897170357256 1101 94-95 -0.05276339304997535 1101 96-97 0.17303267377486264 1101 98-99 0.054744450825797486 1101 100-101 0.6543213189054953 1102 1 0.17840818037846518 1102 2 0.19687895637006037 1102 3 0.19380668177943505 1102 4 0.11691373928695015 1102 5 0.14128593464455008 1102 6 0.09895693260524752 1102 7 0.1163511484109705 1102 8 0.19284879089988038 1102 9 0.11708003017653112 1102 10-11 0.17686238979318603 1102 12-13 0.2091672907515303 1102 14-15 0.2940752105100941 1102 16-17 0.3203437270499805 1102 18-19 0.15675132061917907 1102 20-21 0.04072629596232957 1102 22-23 0.07819040078845774 1102 24-25 0.124096788136562 1102 26-27 0.20457799560666245 1102 28-29 0.05423371323008297 1102 30-31 0.22047648713250823 1102 32-33 0.15482140492158436 1102 34-35 0.14483131484428924 1102 36-37 0.20694609370410433 1102 38-39 0.0840623266561451 1102 40-41 0.27600499653602384 1102 42-43 0.2363353118318443 1102 44-45 0.3994452168433824 1102 46-47 0.42025891493420886 1102 48-49 0.4852294655840623 1102 50-51 0.4254335488808678 1102 52-53 0.36333073185223697 1102 54-55 0.37844726144317065 1102 56-57 0.2360769141085015 1102 58-59 0.4011962608392352 1102 60-61 0.48695396227684995 1102 62-63 0.4395577690786112 1102 64-65 0.29817248399783836 1102 66-67 0.1734164556768718 1102 68-69 0.3408339428332603 1102 70-71 0.2700616590572942 1102 72-73 0.4182536897467166 1102 74-75 0.2950001524308128 1102 76-77 0.11710114460847976 1102 78-79 0.18544607221853582 1102 80-81 0.36757121701843687 1102 82-83 0.19430592537960933 1102 84-85 0.13313368582322838 1102 86-87 0.1419791454261663 1102 88-89 0.10388876935746083 1102 90-91 0.024142801388599366 1102 92-93 0.17722927093300456 1102 94-95 0.030322859436338945 1102 96-97 0.18152898787196747 1102 98-99 0.04259027447375274 1102 100-101 0.7942625907975049 1103 1 0.09569490453692708 1103 2 0.19665579439984882 1103 3 0.18697381461259255 1103 4 0.10176675402898638 1103 5 0.17295064286027184 1103 6 0.16536657149394074 1103 7 0.1428804619102948 1103 8 0.061721913709966714 1103 9 0.2174089538364541 1103 10-11 0.12791851182541336 1103 12-13 0.1469361995909395 1103 14-15 0.20653899340446458 1103 16-17 0.2704297885561928 1103 18-19 0.13111291988968787 1103 20-21 0.09872238155188029 1103 22-23 0.14153276381745172 1103 24-25 0.14436955826531772 1103 26-27 0.03801361150967608 1103 28-29 0.06642206727174482 1103 30-31 0.14907800367291912 1103 32-33 0.0610509339483869 1103 34-35 0.10838023616582149 1103 36-37 0.13200171068555733 1103 38-39 0.1753930980758227 1103 40-41 0.1400092643752302 1103 42-43 0.20166766033396044 1103 44-45 0.29418270522481293 1103 46-47 0.34405484132065567 1103 48-49 0.23364183900771707 1103 50-51 0.3917040585603857 1103 52-53 0.32295549941409263 1103 54-55 0.36722029237245835 1103 56-57 0.08503683517963623 1103 58-59 0.4033735582196485 1103 60-61 0.39370602665854193 1103 62-63 0.43364357645779705 1103 64-65 0.42065837487591295 1103 66-67 0.12675144581876907 1103 68-69 0.2956479149081197 1103 70-71 0.23738284518985964 1103 72-73 0.2698369651985253 1103 74-75 0.3087810714417003 1103 76-77 0.11225044156819308 1103 78-79 0.09943114706054601 1103 80-81 0.3523823221560214 1103 82-83 0.31464641822588035 1103 84-85 0.507472274852308 1103 86-87 0.6284504848624621 1103 88-89 0.601002345453292 1103 90-91 0.535880960857579 1103 92-93 0.29015817035475777 1103 94-95 0.4032983185600578 1103 96-97 0.629904363390029 1103 98-99 0.5563420970076649 1103 100-101 0.6484829680480111 1104 1 0.12175308679471897 1104 2 0.17750022729955006 1104 3 0.15691690227697563 1104 4 0.10178608603467154 1104 5 0.059330999799890094 1104 6 0.07202647468143653 1104 7 0.02326571883265416 1104 8 0.08089926335188835 1104 9 0.09924138836765906 1104 10-11 0.04609470862631326 1104 12-13 0.14474964083498065 1104 14-15 0.13728889007353473 1104 16-17 0.10325136830234527 1104 18-19 -0.015131661022380172 1104 20-21 0.024094440976831777 1104 22-23 0.055749983678992976 1104 24-25 0.038593263878624384 1104 26-27 0.16128332893020314 1104 28-29 0.23077338590513108 1104 30-31 0.2036751273972115 1104 32-33 0.1889833405434942 1104 34-35 0.1654803100630886 1104 36-37 0.2838615585453965 1104 38-39 0.28936003653415554 1104 40-41 0.45081659071528435 1104 42-43 0.4482371823106419 1104 44-45 0.3937487969957516 1104 46-47 0.5425355907272191 1104 48-49 0.7114576817769702 1104 50-51 0.6910386563702602 1104 52-53 0.7254938734453873 1104 54-55 0.7248414318934522 1104 56-57 0.6803041423619405 1104 58-59 0.8340581072638571 1104 60-61 0.8925222816624014 1104 62-63 0.8549262359754337 1104 64-65 0.8045325142613038 1104 66-67 0.7188781189221771 1104 68-69 0.6158363012193178 1104 70-71 0.7203633285771325 1104 72-73 0.7502807354733072 1104 74-75 0.8561353639365841 1104 76-77 0.5298643765135402 1104 78-79 0.5113353332838528 1104 80-81 0.7043691350053258 1104 82-83 0.633495586650124 1104 84-85 0.9156317698894263 1104 86-87 0.8984740862463063 1104 88-89 0.7758864759459669 1104 90-91 0.9608921107960704 1104 92-93 0.8765219997909242 1104 94-95 0.7998002057450115 1104 96-97 0.8932198949412289 1104 98-99 0.8881491548778904 1104 100-101 1.66636439260445 1105 1 -0.035195758046562275 1105 2 0.0714991042891846 1105 3 0.002227461192831015 1105 4 0.044285336876200176 1105 5 0.2002955667862949 1105 6 0.08300899237757875 1105 7 -0.10868072579216914 1105 8 0.021371567763338817 1105 9 -0.03713920159099615 1105 10-11 0.021982570513500832 1105 12-13 0.10160262248569296 1105 14-15 0.08496460773825731 1105 16-17 -0.09047795710591089 1105 18-19 -0.1315088470377006 1105 20-21 -0.18599883288932517 1105 22-23 -0.012781142037070481 1105 24-25 0.06790107054627725 1105 26-27 0.00997193793931217 1105 28-29 0.048005194446410826 1105 30-31 0.011498087431426995 1105 32-33 0.04198244737203538 1105 34-35 0.3130558005073212 1105 36-37 0.12164416584962368 1105 38-39 0.026324097293091597 1105 40-41 0.2520639759340213 1105 42-43 0.0976614212965572 1105 44-45 0.16742540492256808 1105 46-47 0.14759001018032336 1105 48-49 0.05899564156388948 1105 50-51 0.28168217622222613 1105 52-53 0.3691532289072583 1105 54-55 0.17374271462731627 1105 56-57 0.10750194056337392 1105 58-59 0.016441827686641375 1105 60-61 0.2447124428114762 1105 62-63 0.21255368746923864 1105 64-65 0.16073961948388416 1105 66-67 0.1777872860034293 1105 68-69 0.3542774406384339 1105 70-71 0.3799134261859365 1105 72-73 0.44885791625179294 1105 74-75 0.21725462123687578 1105 76-77 0.2672079624078343 1105 78-79 0.12562767673882647 1105 80-81 0.22822204974091065 1105 82-83 0.46176286311724013 1105 84-85 0.4988418921113613 1105 86-87 0.5258865947197968 1105 88-89 0.3643017221208922 1105 90-91 0.4355798644553097 1105 92-93 0.39396415457433775 1105 94-95 0.8196389402927089 1105 96-97 0.6907486902653126 1105 98-99 0.59566488720575 1105 100-101 1.1543732021261839 1106 1 0.1171267901947175 1106 2 0.1599033751208303 1106 3 0.10742828425143358 1106 4 0.17160180938960679 1106 5 0.11265926795769587 1106 6 -0.0022731644248068505 1106 7 0.007530936364958052 1106 8 0.045428209749054815 1106 9 0.062023158059687944 1106 10-11 0.1464556023827157 1106 12-13 0.09736986540139014 1106 14-15 0.18861085455862536 1106 16-17 0.20758024026613242 1106 18-19 0.2472414972535475 1106 20-21 0.1292204603820153 1106 22-23 0.15794762126877515 1106 24-25 0.16635459527854124 1106 26-27 0.16734625138206383 1106 28-29 -0.08051346208863919 1106 30-31 -0.12481046233283166 1106 32-33 -0.04081595187619769 1106 34-35 0.043431199480117755 1106 36-37 -0.0431311942385193 1106 38-39 0.09002931917866874 1106 40-41 0.19425464037562534 1106 42-43 0.26703732252952506 1106 44-45 0.319870277818346 1106 46-47 0.2611477944028735 1106 48-49 0.08789828410046852 1106 50-51 0.3753812413702349 1106 52-53 0.0026499318122716886 1106 54-55 0.10280575637971623 1106 56-57 -0.13261730572232722 1106 58-59 -0.040166944044209174 1106 60-61 0.16061383779859995 1106 62-63 0.15087208801362806 1106 64-65 0.021525251602611206 1106 66-67 0.1749746290471279 1106 68-69 0.1465175210000602 1106 70-71 0.2998580155477413 1106 72-73 0.42621003303538174 1106 74-75 0.22644355207078704 1106 76-77 0.17771654133558812 1106 78-79 0.10179705182996202 1106 80-81 0.3113493483912855 1106 82-83 0.2884085752735537 1106 84-85 0.4905594598759784 1106 86-87 0.6432210513877088 1106 88-89 0.5047482476146428 1106 90-91 0.3803053225651922 1106 92-93 0.45080542415843183 1106 94-95 0.47687472713996826 1106 96-97 0.8493980964641743 1106 98-99 0.7000248295268392 1106 100-101 1.1921966333732463 1107 1 0.07184120630627078 1107 2 0.012829129136090245 1107 3 -0.06613214378452881 1107 4 0.033787434061885335 1107 5 0.017889308158892447 1107 6 -0.06893335746825358 1107 7 -0.08514933785772172 1107 8 0.027199781480035767 1107 9 -0.07838972962942137 1107 10-11 0.03779777868537337 1107 12-13 -0.08081078752824311 1107 14-15 0.060695296499936546 1107 16-17 0.07676320427926697 1107 18-19 0.04580046872428767 1107 20-21 0.11635996048882902 1107 22-23 -0.08834784784233563 1107 24-25 0.0464889876966339 1107 26-27 0.0017075960830723602 1107 28-29 -0.03959563178820247 1107 30-31 0.048116845352005555 1107 32-33 0.012201080226496686 1107 34-35 0.060443881308010816 1107 36-37 -0.010515785720521365 1107 38-39 -0.14634014344586177 1107 40-41 -0.04304896607987274 1107 42-43 -0.1281113442277757 1107 44-45 -0.08318462498020551 1107 46-47 -0.06338853233383901 1107 48-49 0.010525539348954283 1107 50-51 0.08261551532102374 1107 52-53 0.08465261189228102 1107 54-55 0.012347229646884728 1107 56-57 0.007444064225815339 1107 58-59 -0.0940462627601022 1107 60-61 0.1579842520457504 1107 62-63 0.14648102938201646 1107 64-65 0.07852744616088358 1107 66-67 0.1788277295889671 1107 68-69 0.1539829032998803 1107 70-71 0.23154510649567328 1107 72-73 0.2391501584942013 1107 74-75 0.2862773094264419 1107 76-77 0.0737080738364142 1107 78-79 0.17703932717905246 1107 80-81 0.2989199918046985 1107 82-83 0.19969015846285032 1107 84-85 0.45541933830133274 1107 86-87 0.27043940022536717 1107 88-89 -0.04094905871072996 1107 90-91 0.062401222819605806 1107 92-93 0.18187298936400254 1107 94-95 0.41610099969245695 1107 96-97 0.7297131029701696 1107 98-99 0.48981139416920527 1107 100-101 0.9476179691406621 1108 1 0.032284551886547774 1108 2 0.14133320819864892 1108 3 -0.004036177253595952 1108 4 0.05116944247580335 1108 5 0.0739005485516202 1108 6 0.01495253437366273 1108 7 0.053442608955869275 1108 8 0.04865860142706424 1108 9 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-1.7448590261556483 2316 60-61 -1.2406874818494948 2316 62-63 -1.1379109031887182 2316 64-65 -0.918375774790352 2316 66-67 -0.8187753709528742 2316 68-69 -1.5960961153635758 2316 70-71 -1.6571251222821708 2316 72-73 -1.2440978848531827 2316 74-75 -1.461258060832435 2316 76-77 -1.256759265116024 2316 78-79 -1.1738261153254612 2316 80-81 -1.0791015313741106 2316 82-83 -1.4920166446677925 2316 84-85 -1.297105496135753 2316 86-87 -1.2575670717794445 2316 88-89 -1.5264284386023625 2316 90-91 -1.4676785484025494 2316 92-93 -1.339040616897286 2316 94-95 -2.0698261526254242 2316 96-97 -1.9376070355006334 2316 98-99 -2.634872531176974 2316 100-101 -3.61018606457397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 32.0 5 56.0 6 114.0 7 255.0 8 648.0 9 1105.0 10 1842.0 11 2891.0 12 4068.0 13 5576.0 14 7167.0 15 9617.0 16 12422.0 17 16351.0 18 14102.0 19 9367.0 20 8606.0 21 8573.0 22 9242.0 23 10242.0 24 11878.0 25 14227.0 26 17512.0 27 21128.0 28 26224.0 29 32330.0 30 39728.0 31 49050.0 32 60611.0 33 77161.0 34 100853.0 35 137270.0 36 190130.0 37 235035.0 38 130764.0 39 6483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.62219737021002 19.296443551308883 14.909137777512315 22.172221300968783 2 16.27991226808685 20.711851273818027 38.6418056239897 24.366430834105426 3 19.49697099717021 25.01487193632218 29.753381253580464 25.73477581292715 4 13.437990112110935 16.8064255563902 36.200171607992026 33.55541272350684 5 14.104931481015843 37.82978689967312 32.81611453356801 15.249167085743023 6 32.31172623182165 35.95203148032323 16.744036936577334 14.992205351277788 7 29.465500821895418 33.30452246776064 19.490157684486448 17.73981902585749 8 27.950887427043032 33.593128765711086 18.938953442688902 19.517030364556977 9 27.850939915201767 16.255849915296057 19.621849531849627 36.271360637652556 10-11 25.87571935492996 26.38928612961118 25.999946568940214 21.73504794651865 12-13 26.90465236609965 23.85562920307845 27.06919047486217 22.17052795595973 14-15 23.683453723558483 25.08091276036858 25.844859652996423 25.390773863076515 16-17 22.712286315048384 28.532932390851347 26.250247511526965 22.5045337825733 18-19 22.532743851675665 26.189227383788154 29.50036675628328 21.7776620082529 20-21 23.618139216197783 25.27245911738175 29.517124654662492 21.592277011757975 22-23 22.992315740219453 25.302111747264505 29.70377549813718 22.001797014378866 24-25 23.107558294857732 25.508616544141915 29.729748842588954 21.6540763184114 26-27 22.75525654716022 25.845852476444836 29.614049727694397 21.78484124870055 28-29 22.31158187540025 25.9417272037937 29.761872021435842 21.984818899370204 30-31 22.58980940216642 25.53094774029223 29.723526474409574 22.15571638313178 32-33 22.409513769733422 25.801664446069346 29.569751574685743 22.21907020951149 34-35 22.970101756178053 25.58311397477704 29.290653361096926 22.156130907947983 36-37 23.231529539889078 25.39919326481807 29.14744348551715 22.2218337097757 38-39 22.81276666629998 25.579849417518297 29.334078754857945 22.27330516132378 40-41 22.745737887444243 25.6104699815427 29.381841440062484 22.26195069095058 42-43 22.965595061480048 25.749995285272448 28.835784467801556 22.44862518544595 44-45 22.916570083345842 25.964425383589663 28.54294467681191 22.576059856252588 46-47 23.323951453091386 26.343675402658672 27.968571486941585 22.36380165730836 48-49 23.61049232986266 26.511299534912226 27.33971994157133 22.53848819365378 50-51 23.44848151260603 26.440511226643 27.313561429583366 22.7974458311676 52-53 23.685416036427497 26.664754668353414 27.100965297230616 22.548863997988473 54-55 23.246467003759886 26.588404464717186 27.141347351382354 23.023781180140574 56-57 23.470147171735984 26.864716269836592 26.886013616155807 22.779122942271616 58-59 23.681069692809935 26.270179648437207 26.99053400147215 23.05821665728071 60-61 23.508482648805924 26.284972358495462 27.62629660426828 22.580248388430338 62-63 23.17128326194255 26.59195029456803 27.63835148785047 22.598414955638955 64-65 23.066488525620873 26.51218170386671 27.447461490097453 22.973868280414962 66-67 23.25983601694599 26.733072377982158 27.071701431246986 22.935390173824864 68-69 23.14822454125503 26.4458530460289 27.451347849420543 22.95457456329553 70-71 23.2170498779727 26.55641778754206 27.339576919631703 22.886955414853542 72-73 23.35106249233893 26.626504320058338 26.972666869914224 23.04976631768851 74-75 23.27108344507616 26.979007016519603 26.746773143533574 23.00313639487066 76-77 23.33126936444164 26.86859249632061 26.90929563918471 22.89084250005304 78-79 23.33595804856352 26.7292276186053 26.820542561643478 23.1142717711877 80-81 23.227216016818446 27.365339463420863 26.596445728095254 22.810998791665437 82-83 23.450435266466858 27.185478569124115 26.415347912556104 22.948738251852923 84-85 23.25604793544467 26.91676144916213 26.469508595665342 23.35768201972786 86-87 23.345680618037463 26.743220617699535 26.52192039243068 23.389178371832315 88-89 23.19975629888762 26.84583939310562 26.406627097991432 23.54777721001533 90-91 23.393245662825596 26.850563778623776 26.538888637752827 23.2173019207978 92-93 23.277989740881004 26.569954898234126 26.581077066011442 23.570978294873427 94-95 23.02418909523658 26.615943729216703 26.679809197492073 23.68005797805465 96-97 23.31574728769872 26.62483543793263 26.533439365880295 23.525977908488358 98-99 23.07196980865053 26.72127055262356 26.214621969317253 23.992137669408656 100-101 22.928773898309082 26.79425536348232 26.288631422516996 23.9883393156916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 455.0 1 328.5 2 248.0 3 313.5 4 304.0 5 292.0 6 443.0 7 909.5 8 1424.0 9 1671.0 10 1592.0 11 1403.5 12 1421.0 13 1547.5 14 1756.5 15 1927.5 16 2015.5 17 2169.0 18 2321.0 19 2438.0 20 2583.0 21 2805.5 22 3167.5 23 3625.0 24 4202.0 25 4958.0 26 5977.0 27 7168.0 28 8498.5 29 10266.0 30 12507.5 31 14995.0 32 17906.5 33 20398.5 34 22518.0 35 24169.5 36 26487.5 37 29112.0 38 30578.0 39 31384.5 40 32070.5 41 34564.5 42 37111.5 43 39457.5 44 41718.5 45 43807.0 46 44252.0 47 44896.5 48 49677.0 49 49962.0 50 46616.5 51 44654.0 52 43599.5 53 44470.5 54 44865.5 55 42819.5 56 39104.5 57 35989.5 58 34276.0 59 31846.5 60 27502.0 61 22920.5 62 19715.0 63 16180.5 64 12222.5 65 9678.0 66 7281.0 67 5889.5 68 4412.5 69 2632.0 70 1959.0 71 1627.0 72 1434.0 73 1311.5 74 1090.5 75 891.0 76 665.0 77 431.0 78 288.0 79 219.0 80 155.5 81 119.0 82 87.5 83 54.5 84 35.0 85 23.5 86 13.0 87 5.0 88 2.0 89 2.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019408046717604276 2 0.012179138628456125 3 0.010764787045796705 4 0.0 5 0.002200102461914655 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011393387749200892 14-15 7.857508792552339E-5 16-17 0.0 18-19 0.0019250896541753231 20-21 0.0 22-23 1.1786263188828508E-4 24-25 0.0 26-27 0.0013357764947338976 28-29 0.0025929779015422716 30-31 0.0011393387749200892 32-33 0.008839697391621382 34-35 0.001414351582659421 36-37 5.893131594414254E-4 38-39 7.857508792552339E-4 40-41 7.857508792552339E-5 42-43 0.004714505275531403 44-45 0.0014929266705849444 46-47 1.5715017585104678E-4 48-49 3.9287543962761697E-4 50-51 2.750128077393319E-4 52-53 2.3572526377657016E-4 54-55 0.001807227022287038 56-57 0.01642219337643439 58-59 0.02966209569188508 60-61 0.02404397690521016 62-63 0.0166186310962482 64-65 0.02105812356404027 66-67 0.0028679907092816035 68-69 8.643259671807572E-4 70-71 1.5715017585104678E-4 72-73 0.0 74-75 0.0022393900058774166 76-77 0.003064428429095412 78-79 0.011707688100902985 80-81 0.020075934964971228 82-83 0.022511762690662452 84-85 0.03590881518196419 86-87 0.014811404073961159 88-89 0.048559404337973455 90-91 0.052684596454063426 92-93 0.03410158815967715 94-95 0.036340978165554566 96-97 0.05747767681752036 98-99 0.06132785612587101 100-101 0.06859605175898192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1272668.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.13151879441734 #Duplication Level Percentage of deduplicated Percentage of total 1 74.91808925659478 36.80839510352222 2 13.396138041553563 13.16345215922396 3 4.696087709988447 6.92177764750589 4 2.1656205307337806 4.256009032292911 5 1.2014259792591016 2.9513941540034905 6 0.7499080809699814 2.210647378455725 7 0.49056348283506285 1.6871490283736004 8 0.34563694893865965 1.358533460225986 9 0.2557011396390344 1.1306686813136237 >10 1.5493894616185555 14.378920608231835 >50 0.14425632231560814 4.9333738797256 >100 0.08206404673883988 7.271069805825167 >500 0.004159186536855646 1.3808365787659747 >1k 6.398748518239456E-4 0.6055749239792723 >5k 3.199374259119728E-4 0.9421975585546419 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6440 0.5060235662403706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5548 0.43593458781080374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3423 0.26896252596906656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1602 0.12587729085668847 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.12139851084493364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.857508792552339E-5 2 0.0 0.0 0.0 0.0 7.857508792552339E-5 3 0.0 0.0 0.0 0.0 7.857508792552339E-5 4 0.0 0.0 0.0 0.0 7.857508792552339E-5 5 0.0 0.0 0.0 0.0 7.857508792552339E-5 6 0.0 0.0 0.0 0.0 1.5715017585104678E-4 7 0.0 0.0 0.0 0.0 1.5715017585104678E-4 8 0.0 0.0 0.0 0.0 1.5715017585104678E-4 9 0.0 0.0 0.0 0.0 1.5715017585104678E-4 10-11 0.0 0.0 0.0 0.0 1.5715017585104678E-4 12-13 0.0 0.0 0.0 0.0 1.5715017585104678E-4 14-15 0.0 0.0 0.0 0.0 1.5715017585104678E-4 16-17 0.0 0.0 0.0 0.0 1.5715017585104678E-4 18-19 0.0 0.0 0.0 0.0 2.3572526377657016E-4 20-21 0.0 0.0 0.0 3.9287543962761694E-5 2.3572526377657016E-4 22-23 0.0 0.0 0.0 1.5715017585104678E-4 2.3572526377657016E-4 24-25 0.0 0.0 0.0 3.5358789566485526E-4 2.3572526377657016E-4 26-27 0.0 0.0 0.0 7.464633352924721E-4 2.3572526377657016E-4 28-29 0.0 0.0 0.0 0.0016107893024732295 2.7501280773933184E-4 30-31 0.0 0.0 0.0 0.0032608661489092203 3.1430035170209355E-4 32-33 0.0 0.0 0.0 0.005382393522898352 4.714505275531403E-4 34-35 0.0 0.0 0.0 0.007268195633110913 5.500256154786637E-4 36-37 0.0 0.0 0.0 0.009900461078615946 5.500256154786637E-4 38-39 0.0 0.0 0.0 0.012729164243934788 5.500256154786637E-4 40-41 0.0 0.0 0.0 0.01701150653587581 5.500256154786637E-4 42-43 0.0 0.0 0.0 0.02431898971294949 5.500256154786637E-4 44-45 0.0 0.0 0.0 0.03618382798970352 5.500256154786637E-4 46-47 0.0 0.0 0.0 0.047262915387202314 5.500256154786637E-4 48-49 0.0 0.0 0.0 0.0695389528140882 5.500256154786637E-4 50-51 0.0 0.0 0.0 0.09452583077440463 5.500256154786637E-4 52-53 0.0 0.0 0.0 0.12249856207589097 6.286007034041871E-4 54-55 0.0 0.0 0.0 0.1672863621934393 7.071757913297105E-4 56-57 0.0 0.0 0.0 0.21062052318436544 7.071757913297105E-4 58-59 0.0 0.0 0.0 0.2607514292808494 7.464633352924722E-4 60-61 0.0 0.0 0.0 0.3180326683785559 7.857508792552339E-4 62-63 0.0 0.0 0.0 0.39523269226538266 7.857508792552339E-4 64-65 0.0 0.0 0.0 0.451885330659685 7.857508792552339E-4 66-67 0.0 0.0 0.0 0.5469218995056055 7.857508792552339E-4 68-69 0.0 0.0 0.0 0.6766493696706447 7.857508792552339E-4 70-71 0.0 0.0 0.0 0.7496456263534559 7.857508792552339E-4 72-73 0.0 0.0 0.0 0.8631473408618744 8.643259671807572E-4 74-75 0.0 0.0 0.0 1.0103184805463798 8.643259671807572E-4 76-77 0.0 0.0 0.0 1.1161591239820599 8.643259671807572E-4 78-79 0.0 0.0 0.0 1.2169316742465435 8.643259671807572E-4 80-81 0.0 0.0 0.0 1.3699566579815003 8.643259671807572E-4 82-83 0.0 0.0 0.0 1.5741733114999357 8.643259671807572E-4 84-85 0.0 0.0 0.0 1.8106057510678355 8.643259671807572E-4 86-87 0.0 0.0 0.0 2.1543324731980373 8.643259671807572E-4 88-89 0.0 0.0 0.0 2.533182259630949 8.643259671807572E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6255 0.0 32.80688 1 GTATCAA 9130 0.0 30.644535 1 GTAAGGT 495 0.0 28.779818 4 AGGTAAG 485 0.0 27.41931 2 GTACATG 7525 0.0 26.323162 1 TAAGGTG 580 0.0 26.199558 5 TACATGG 7875 0.0 24.96836 2 ATCAACG 11355 0.0 24.593105 3 TCAACGC 11410 0.0 24.471674 4 GGTAAGG 565 0.0 24.376577 3 CAACGCA 11510 0.0 24.30032 5 TATCAAC 11685 0.0 24.062084 2 ACATGGG 7730 0.0 23.838308 3 TAACGCC 100 0.0016564407 23.74335 4 AACGCAG 11840 0.0 23.662205 6 GTACTAC 205 2.6688213E-8 23.171528 1 GAGTACT 6580 0.0 22.517376 12-13 GTACTTT 6775 0.0 21.727377 14-15 ACGCAGA 13105 0.0 21.271109 7 TAGGACA 405 0.0 21.105202 4 >>END_MODULE