##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936603_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54991 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.631939771962685 31.0 31.0 34.0 30.0 34.0 2 31.623538397192267 31.0 31.0 34.0 30.0 34.0 3 31.75301412958484 31.0 31.0 34.0 30.0 34.0 4 35.443999927260826 37.0 35.0 37.0 33.0 37.0 5 35.31270571548072 37.0 35.0 37.0 33.0 37.0 6 35.51375679656671 37.0 35.0 37.0 33.0 37.0 7 35.45309232419851 37.0 35.0 37.0 33.0 37.0 8 35.51246567620156 37.0 35.0 37.0 33.0 37.0 9 36.90382062519321 39.0 37.0 39.0 33.0 39.0 10-11 36.80021276208834 39.0 36.0 39.0 32.5 39.0 12-13 36.763597679620304 39.0 37.0 39.0 32.0 39.0 14-15 37.34809332436217 39.0 36.0 40.0 32.0 41.0 16-17 37.54654397992399 39.0 36.5 40.5 32.5 41.0 18-19 37.98020585186667 39.5 37.0 41.0 33.0 41.0 20-21 38.056236475059556 40.0 37.0 41.0 33.0 41.0 22-23 37.975541452237636 39.5 37.0 41.0 33.0 41.0 24-25 37.69182229819425 39.5 36.5 41.0 32.5 41.0 26-27 37.2714626029714 39.0 36.0 40.5 31.5 41.0 28-29 37.366241748649784 39.0 36.0 40.5 32.5 41.0 30-31 37.33653688785438 39.0 36.0 41.0 33.0 41.0 32-33 36.86702369478642 39.0 35.0 40.0 31.0 41.0 34-35 36.39423723882089 38.0 35.0 40.0 30.5 41.0 36-37 36.075985161208195 38.0 35.0 40.0 30.5 41.0 38-39 35.7626793475296 38.0 35.0 40.0 30.0 41.0 40-41 35.7631885217581 38.0 35.0 40.0 30.0 41.0 42-43 35.18290265679839 38.0 35.0 40.0 28.0 41.0 44-45 34.60588096233929 38.0 34.0 40.0 24.5 41.0 46-47 33.81435143932643 38.0 33.0 40.0 21.0 41.0 48-49 32.9293247986034 37.5 33.0 40.0 8.0 41.0 50-51 32.414058664145045 37.0 33.0 40.0 2.0 41.0 52-53 32.307295739302795 38.0 32.5 40.0 2.0 41.0 54-55 32.006219199505374 37.5 31.5 40.0 2.0 41.0 56-57 31.286865123383826 36.5 30.0 39.5 2.0 40.5 58-59 30.87675255950974 36.0 30.0 39.0 2.0 40.0 60-61 30.020321507155714 35.0 28.0 39.0 2.0 40.0 62-63 29.801112909385175 35.0 28.0 38.0 2.0 40.0 64-65 29.537524322161808 35.0 28.5 38.5 2.0 39.5 66-67 28.739839246422143 34.5 26.5 37.5 2.0 39.0 68-69 28.058900547362295 34.0 26.0 37.0 2.0 39.0 70-71 27.140222945572912 33.5 25.0 35.5 2.0 38.0 72-73 26.37474313978651 32.5 23.5 35.0 2.0 37.0 74-75 26.00721027077158 32.0 22.0 35.0 2.0 37.0 76-77 24.709279700314596 30.5 20.5 33.5 2.0 35.0 78-79 25.470704297066796 32.0 21.0 34.0 2.0 35.0 80-81 25.100752850466442 32.0 20.0 34.0 2.0 35.0 82-83 24.648806168282082 31.5 19.0 34.0 2.0 35.0 84-85 24.29782146169373 31.0 17.5 34.0 2.0 35.0 86-87 24.33308177701806 31.0 14.0 34.0 2.0 35.0 88-89 23.84251968503937 31.0 7.0 34.0 2.0 35.0 90-91 23.12131075994254 30.5 2.0 34.0 2.0 35.0 92-93 22.635931334218327 30.0 2.0 34.0 2.0 35.0 94-95 21.854221599898164 29.0 2.0 34.0 2.0 35.0 96-97 21.026167918386648 29.0 2.0 34.0 2.0 34.5 98-99 20.314387808914187 29.0 2.0 34.0 2.0 35.0 100-101 17.222609154225236 22.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5991116346562784 1101 2 0.7895208384243979 1101 3 0.4815913940209704 1101 4 0.6254036879097171 1101 5 0.7722656751467198 1101 6 0.5598920542313337 1101 7 0.5709917961984274 1101 8 0.13295612278822944 1101 9 0.5732206073083717 1101 10-11 0.33986840863862966 1101 12-13 0.8143365609022695 1101 14-15 0.7874568245092988 1101 16-17 1.4702418285938919 1101 18-19 0.7536549118697451 1101 20-21 1.0524731721263763 1101 22-23 0.4443154327138288 1101 24-25 1.032956351973013 1101 26-27 0.9737341658093186 1101 28-29 1.5080021913040085 1101 30-31 1.1169670208387572 1101 32-33 0.982424121690002 1101 34-35 1.4590658151187554 1101 36-37 2.0086598125461492 1101 38-39 1.3567578028654523 1101 40-41 0.9415925433465446 1101 42-43 0.10284521761252563 1101 44-45 0.18868893440317436 1101 46-47 -0.03928092578752995 1101 48-49 0.7571968985511148 1101 50-51 0.7124755151376618 1101 52-53 1.4090785193050053 1101 54-55 1.6483820165475578 1101 56-57 0.7441731693719049 1101 58-59 -0.3151599941623573 1101 60-61 -0.6421218434032134 1101 62-63 -0.9279957810263184 1101 64-65 -2.216670430790387 1101 66-67 -2.1658145321808675 1101 68-69 -2.2185136158633156 1101 70-71 -2.0878612550716795 1101 72-73 -1.9323841363412413 1101 74-75 -1.431364408345182 1101 76-77 -0.7095952314545038 1101 78-79 -1.3381204744043735 1101 80-81 -1.7361440479298373 1101 82-83 -1.2290279929059764 1101 84-85 -0.6619308538628452 1101 86-87 -1.4796342652217511 1101 88-89 -1.6533027214293945 1101 90-91 -1.2110923500442716 1101 92-93 -0.5229419331273135 1101 94-95 -0.2484141910940103 1101 96-97 -0.4243670194640501 1101 98-99 -0.1090857904980922 1101 100-101 0.4202452451121239 1102 1 0.22630461711241878 1102 2 0.3437188334118666 1102 3 0.16141595542448073 1102 4 0.4311680989372846 1102 5 -0.07140600405127628 1102 6 -0.23052147960325442 1102 7 -0.008895421846688123 1102 8 -0.12424939099623344 1102 9 0.12553890304771897 1102 10-11 0.27595863420254574 1102 12-13 0.2958528516290855 1102 14-15 0.4003954210005318 1102 16-17 0.36513531230315976 1102 18-19 0.16139300710784 1102 20-21 0.7333942247579515 1102 22-23 0.2085698186787397 1102 24-25 0.5995290336772783 1102 26-27 0.7765223863606963 1102 28-29 0.9926513141110291 1102 30-31 0.5035584995354938 1102 32-33 1.002944171815308 1102 34-35 1.005431729905716 1102 36-37 0.37551444913763277 1102 38-39 -0.6106607435004534 1102 40-41 -0.40395883509957287 1102 42-43 -0.1476247071994976 1102 44-45 0.6659759018217173 1102 46-47 -0.5522821789203576 1102 48-49 0.4119588033130128 1102 50-51 0.8153890489722571 1102 52-53 0.9574807749441021 1102 54-55 0.26993590125933764 1102 56-57 -0.6327065298762165 1102 58-59 0.20144401586270533 1102 60-61 0.2286488332885135 1102 62-63 -0.389931871251882 1102 64-65 -0.22982832552722954 1102 66-67 0.06256892646574741 1102 68-69 -0.7506251446853689 1102 70-71 -1.0192522325152886 1102 72-73 -1.2381485473688123 1102 74-75 -0.6697729296484383 1102 76-77 0.149583966540483 1102 78-79 0.6770737611846016 1102 80-81 0.6344699871578818 1102 82-83 1.3425572201266078 1102 84-85 0.6847170158113407 1102 86-87 0.9338995693647156 1102 88-89 0.7372047973675961 1102 90-91 0.2569527627376864 1102 92-93 1.6943199716345916 1102 94-95 1.8367988414874432 1102 96-97 0.5818986446963486 1102 98-99 1.253696164389126 1102 100-101 0.9344996310770348 1103 1 0.2613923364106654 1103 2 -0.16505309641269506 1103 3 0.14387209577535742 1103 4 0.1855540638495654 1103 5 0.10403259243994967 1103 6 0.4887767660107798 1103 7 0.5875958062234901 1103 8 0.7880313107581571 1103 9 -0.17270671098737012 1103 10-11 0.468941090342895 1103 12-13 -0.34449802556390097 1103 14-15 -0.204867736894208 1103 16-17 -0.28398749471438123 1103 18-19 0.08244563868679222 1103 20-21 -0.2929215647157264 1103 22-23 -0.7212547427247671 1103 24-25 -1.4881902645683311 1103 26-27 -0.02172322767439283 1103 28-29 -0.04243640518722458 1103 30-31 0.021102359184617114 1103 32-33 -0.04968740713206188 1103 34-35 0.040519449203962665 1103 36-37 0.6386723438744752 1103 38-39 -0.5404853049039673 1103 40-41 -0.36887111580132625 1103 42-43 0.387463012098749 1103 44-45 -0.018234624494070317 1103 46-47 0.4652616807287586 1103 48-49 0.5961693296288075 1103 50-51 1.7978451893231266 1103 52-53 0.98379656441778 1103 54-55 0.47169028722424855 1103 56-57 0.7269425929308007 1103 58-59 0.5084615597223525 1103 60-61 0.7374207631130751 1103 62-63 0.2504190059411009 1103 64-65 1.39297869201663 1103 66-67 0.6152005054131173 1103 68-69 0.8897257325076104 1103 70-71 1.0158354867829544 1103 72-73 1.744307592982068 1103 74-75 1.5319814563164726 1103 76-77 1.3074787033825892 1103 78-79 1.7472491997810913 1103 80-81 1.9414875310175326 1103 82-83 1.0355396762669606 1103 84-85 1.404015261425375 1103 86-87 1.0128469377857634 1103 88-89 0.7459767271921578 1103 90-91 0.6166018855447035 1103 92-93 0.40484628742406414 1103 94-95 0.7227637537681453 1103 96-97 1.24856531136302 1103 98-99 1.5431698485996534 1103 100-101 0.6713417363401923 1104 1 0.4508065499105136 1104 2 0.7347387560999472 1104 3 0.7231168177497871 1104 4 0.19804291309131372 1104 5 0.4456918252677866 1104 6 0.6419138459988858 1104 7 0.3648779947456404 1104 8 0.47224183707394474 1104 9 0.9197702441122431 1104 10-11 0.4512485539170896 1104 12-13 0.548900725551178 1104 14-15 0.04699072281438532 1104 16-17 0.8096788745987382 1104 18-19 0.19811617094964618 1104 20-21 0.5090409687365423 1104 22-23 0.12545950051043775 1104 24-25 0.5903110735226562 1104 26-27 0.4699508728311059 1104 28-29 0.38723947943960724 1104 30-31 0.2882745268920246 1104 32-33 0.6944398601887869 1104 34-35 0.4917534664504686 1104 36-37 -0.35181234241752435 1104 38-39 0.798646422720175 1104 40-41 -0.17856484164134656 1104 42-43 -0.7332625305714942 1104 44-45 -0.4957844312142612 1104 46-47 -1.1193354052064208 1104 48-49 -1.4552728351050632 1104 50-51 -1.6502666156129422 1104 52-53 -0.6963996889274426 1104 54-55 -0.4145719786098283 1104 56-57 -1.0345798572624787 1104 58-59 -1.2839856600219264 1104 60-61 -2.338701746550914 1104 62-63 -2.5549630933749867 1104 64-65 -3.4073483968920826 1104 66-67 -2.571686202883047 1104 68-69 -2.8031080468560496 1104 70-71 -2.7703673083404468 1104 72-73 -1.8185826835567411 1104 74-75 -1.359633173478354 1104 76-77 -1.2787538270961498 1104 78-79 -1.16696430007023 1104 80-81 -2.128093200472211 1104 82-83 -2.021552205981031 1104 84-85 -1.5809683841291893 1104 86-87 -1.103120948030469 1104 88-89 -1.013018217797434 1104 90-91 -0.769809057066059 1104 92-93 -1.4356235311902665 1104 94-95 -1.5861865881884398 1104 96-97 -1.889258060626279 1104 98-99 -1.6593279212486962 1104 100-101 -1.4028479752268588 1105 1 0.2684664733659545 1105 2 0.5400968752907609 1105 3 0.4896559101500024 1105 4 0.3604267293843719 1105 5 0.44104447699003657 1105 6 0.5385786901760312 1105 7 0.3722590773044132 1105 8 0.23030635320297677 1105 9 0.5392344321931617 1105 10-11 0.745398362555683 1105 12-13 0.6962490032985826 1105 14-15 1.1523761793480034 1105 16-17 0.6888478193773011 1105 18-19 0.8979521468927913 1105 20-21 0.80045704309412 1105 22-23 0.21649285206866864 1105 24-25 0.6340508220191055 1105 26-27 1.2004046727217599 1105 28-29 1.1330021913040085 1105 30-31 1.176874854940145 1105 32-33 1.4746476239941515 1105 34-35 1.4325681192662145 1105 36-37 1.7016321627765691 1105 38-39 2.125190982588954 1105 40-41 2.8016155848212065 1105 42-43 2.8744466001010096 1105 44-45 2.894046077260313 1105 46-47 3.0502928069313597 1105 48-49 3.81249643772162 1105 50-51 3.901127588870388 1105 52-53 3.1599425746045426 1105 54-55 3.746308283828668 1105 56-57 3.8749335380354957 1105 58-59 2.7038492224275004 1105 60-61 3.54825834092858 1105 62-63 3.7900341728907314 1105 64-65 3.2188134401773567 1105 66-67 3.2633329332569225 1105 68-69 4.031774402569862 1105 70-71 4.000272385481317 1105 72-73 4.195496047990552 1105 74-75 4.084188587046523 1105 76-77 3.2152319574855888 1105 78-79 3.245692889650929 1105 80-81 2.9130495004572587 1105 82-83 3.6819743112414898 1105 84-85 3.355062233694756 1105 86-87 3.567600757819722 1105 88-89 3.4524000435475592 1105 90-91 3.088446820462643 1105 92-93 3.5787285737851278 1105 94-95 2.1087286660488473 1105 96-97 2.6666468054207435 1105 98-99 0.4001307993636587 1105 100-101 0.6762936322088997 1106 1 -0.24410870432677356 1106 2 0.29406078999824814 1106 3 0.28243885164809157 1106 4 0.21499206563368745 1106 5 -0.14752851371526532 1106 6 -0.30723869637399304 1106 7 -0.21139319169503779 1106 8 -0.45996155275656037 1106 9 0.4282448203834264 1106 10-11 0.02751974035776783 1106 12-13 0.15907021707660363 1106 14-15 0.23343140078048208 1106 16-17 0.13171277290381767 1106 18-19 0.17269244213609625 1106 20-21 0.29717656195688136 1106 22-23 0.39664594118840313 1106 24-25 0.4547178531836664 1106 26-27 0.19876443215314055 1106 28-29 -0.23987916462819925 1106 30-31 -0.016810218870681126 1106 32-33 0.6096940974769254 1106 34-35 0.7205670257725032 1106 36-37 0.4786961321587455 1106 38-39 0.798646422720175 1106 40-41 0.4400792261552624 1106 42-43 0.9531781473946168 1106 44-45 0.605910484039974 1106 46-47 2.185749340556292 1106 48-49 1.9091339445559612 1106 50-51 2.061597791166726 1106 52-53 1.7612274297166266 1106 54-55 1.1532246315596666 1106 56-57 2.7959286173137947 1106 58-59 1.749912645062821 1106 60-61 2.7884168975168855 1106 62-63 1.8009691100148437 1106 64-65 1.7282448234469001 1106 66-67 1.93678837338814 1106 68-69 1.3240105972117462 1106 70-71 1.3906496408120965 1106 72-73 2.639044435087328 1106 74-75 1.8776549621148675 1106 76-77 1.433110579683511 1106 78-79 1.8160865473873962 1106 80-81 0.8719067995277889 1106 82-83 0.5038715228325259 1106 84-85 1.1308960226504716 1106 86-87 -0.7302395920982683 1106 88-89 -0.9706453364415033 1106 90-91 0.4844282310695327 1106 92-93 -0.2746065820377268 1106 94-95 -0.23025438479861293 1106 96-97 5.724478483024598E-4 1106 98-99 0.5016890279038471 1106 100-101 0.8344401603663592 1107 1 0.26290473810455595 1107 2 0.6029444837445972 1107 3 0.12814311815890278 1107 4 0.5712164957914894 1107 5 0.3209109953437661 1107 6 0.5580447635909351 1107 7 0.3105238159028616 1107 8 0.6618970094877383 1107 9 0.12432898169260653 1107 10-11 -0.11333356180471554 1107 12-13 0.1228340978480773 1107 14-15 0.0611637210610283 1107 16-17 1.0050324689879844 1107 18-19 0.5550711680273821 1107 20-21 0.7270421376436147 1107 22-23 0.5326775016793519 1107 24-25 -0.19826285984964187 1107 26-27 0.6464558406861656 1107 28-29 0.5985194326833181 1107 30-31 0.2819916513806291 1107 32-33 0.7124118064190625 1107 34-35 1.2398539924586558 1107 36-37 1.5573051327432026 1107 38-39 1.7570041082841783 1107 40-41 1.012405351228324 1107 42-43 0.8260595033268174 1107 44-45 1.0837012496741067 1107 46-47 0.9610269559858651 1107 48-49 0.5441427113590009 1107 50-51 0.8544090126746084 1107 52-53 0.36628295280254264 1107 54-55 0.8355741347741628 1107 56-57 -0.23796968777095273 1107 58-59 -0.2739038365268911 1107 60-61 0.16422052112880436 1107 62-63 0.29337121404757127 1107 64-65 0.5764330174854742 1107 66-67 1.2485943348142037 1107 68-69 0.4597499309347164 1107 70-71 0.22802544443570838 1107 72-73 0.9407685730183637 1107 74-75 0.3586602221966899 1107 76-77 0.4274121577080585 1107 78-79 1.0882957817532635 1107 80-81 1.4664421589667143 1107 82-83 1.566846391330479 1107 84-85 1.1142390968760658 1107 86-87 1.660759823448199 1107 88-89 2.2952810224129685 1107 90-91 2.700237699216814 1107 92-93 1.5678831900253947 1107 94-95 2.565625217772986 1107 96-97 3.139672389402943 1107 98-99 2.716037362211271 1107 100-101 2.0603314520086755 1108 1 0.21351841431729568 1108 2 0.3618574001677395 1108 3 -0.0904425042841126 1108 4 -0.07314352758665166 1108 5 0.2253528422169353 1108 6 0.40462571040566786 1108 7 0.1275898591524225 1108 8 0.47224183707394474 1108 9 -0.3344670440233486 1108 10-11 -0.2945141579473258 1108 12-13 -0.17143825749966624 1108 14-15 0.5300415702720116 1108 16-17 0.21645853561567918 1108 18-19 0.596421255695418 1108 20-21 -0.474009878721084 1108 22-23 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1.0225296324593423 2316 86-87 0.7088155828025613 2316 88-89 0.1907322329778971 2316 90-91 -0.3091166661536917 2316 92-93 -1.1496516595710844 2316 94-95 -0.7636117594830694 2316 96-97 -0.8225433112573626 2316 98-99 0.47374095722227594 2316 100-101 0.11572396851637734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 4.0 6 6.0 7 15.0 8 39.0 9 74.0 10 130.0 11 216.0 12 307.0 13 440.0 14 588.0 15 840.0 16 1184.0 17 1478.0 18 1229.0 19 810.0 20 721.0 21 758.0 22 804.0 23 757.0 24 832.0 25 867.0 26 928.0 27 1072.0 28 1282.0 29 1531.0 30 1844.0 31 2355.0 32 2795.0 33 3440.0 34 4437.0 35 5952.0 36 8200.0 37 7391.0 38 1657.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.99154284051434 18.64394449193387 14.300783878653403 12.063728788898388 2 14.330144410898113 30.24989996726201 27.519915608744682 27.900040013095197 3 36.753473485124026 23.228704444606095 30.379355495744527 9.638466574525351 4 11.900345517366793 17.01763957083106 41.194762684124385 29.887252227677756 5 8.722061578191209 45.499845418007894 32.24580355356721 13.532289450233693 6 24.83679147496863 37.291556800203665 11.927406302849556 25.94424542197814 7 20.021458056772925 38.93546216653635 21.063446745831136 19.979633030859596 8 25.013183975559638 18.77943663508574 18.552126711643723 37.6552526777109 9 16.679092942481496 27.99549017111891 28.917459220599735 26.40795766579986 10-11 27.25627830008547 22.836464148678875 22.168172973759344 27.739084577476312 12-13 37.29973995744758 22.90374788601771 21.779927624520376 18.016584532014328 14-15 25.717844738229896 27.328108235893144 33.769162226546165 13.1848847993308 16-17 16.554219934896068 29.033988615905 32.676535307061414 21.73525614213752 18-19 15.749227132205856 35.937443171485725 29.92453173304237 18.38879796326605 20-21 21.915092881368263 24.46830758599369 26.211367624728354 27.405231907909695 22-23 17.415577094433633 25.689658307723086 31.609717953846996 25.28504664399629 24-25 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29.33522463787883 19.04125408039863 50-51 13.154879889436453 31.420596097543235 36.96513975014093 18.45938426287938 52-53 21.342777388821705 35.11151926241806 22.081086733162998 21.46461661559724 54-55 16.95186485061192 26.225200487352474 28.241894128130056 28.58104053390555 56-57 25.37327004564715 23.972939058322876 25.55967774201175 25.09411315401822 58-59 31.82971294519863 23.615417488793312 24.502859636839762 20.052009929168296 60-61 21.398110514016565 28.428671449484895 34.17896469261755 15.994253343880994 62-63 16.980205671888783 28.206690246497125 32.546530764404764 22.266573317209335 64-65 15.90408990889087 36.667333466693336 26.6544217934496 20.774154830966193 66-67 21.29387161302055 25.474631751227495 22.9396253864339 30.29187124931806 68-69 17.617588492557807 26.586410379974723 28.53674725634428 27.259253871123196 70-71 26.9399334412337 26.433468511884193 23.608358035243413 23.018240011638692 72-73 22.351743075887907 20.159486442743095 35.722599065267595 21.7661714161014 74-75 16.573766765173904 35.43077972266424 26.133212093657647 21.862241418504205 76-77 19.76250443258381 24.645165983215282 25.619879797419554 29.972449786781354 78-79 20.943660435004 25.537389975994763 29.292754782861714 24.226194806139524 80-81 25.665808927158825 23.334454759545004 26.51415270187945 24.485583611416725 82-83 20.305510092744136 22.163120567375884 34.07801418439716 23.453355155482818 84-85 18.85342789598109 27.157665030005457 29.738134206219314 24.250772867794144 86-87 18.775061146925378 28.80224766095962 25.851738027477474 26.57095316463753 88-89 17.757774140752865 28.30787415893799 20.9328968903437 33.001454809965445 90-91 21.93447482563902 20.186773117037816 25.32712574904749 32.551626308275665 92-93 25.773336485478914 26.80808889050537 26.327083598537886 21.091491025477822 94-95 22.107253914276882 24.644941899583568 27.23354730774127 26.014256878398285 96-97 12.11470967976578 27.40266589078213 33.70460620828863 26.778018221163464 98-99 19.365339152573195 30.231860338243315 21.580287324968175 28.82251318421531 100-101 16.31296599381706 24.038006910347338 25.185488270594654 34.46353882524095 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 119.0 1 82.5 2 47.5 3 56.5 4 55.0 5 47.5 6 70.5 7 103.0 8 125.5 9 150.0 10 130.5 11 96.5 12 84.5 13 74.0 14 76.5 15 71.5 16 67.0 17 69.5 18 84.5 19 99.5 20 104.5 21 117.5 22 129.5 23 167.0 24 198.0 25 225.0 26 270.0 27 302.0 28 336.5 29 401.5 30 505.5 31 570.5 32 606.0 33 656.5 34 683.5 35 673.5 36 632.0 37 597.5 38 522.0 39 428.5 40 361.5 41 312.5 42 274.5 43 246.5 44 261.0 45 362.5 46 1143.5 47 5755.0 48 14222.5 49 13335.0 50 5312.0 51 1835.0 52 674.5 53 289.0 54 151.0 55 115.5 56 89.5 57 76.5 58 64.5 59 57.0 60 56.0 61 43.5 62 36.5 63 27.5 64 21.5 65 19.5 66 12.0 67 11.5 68 10.0 69 4.5 70 4.5 71 5.0 72 4.5 73 3.0 74 2.5 75 2.5 76 3.0 77 2.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014547835100289138 2 0.01636631448782528 3 0.005455438162608427 4 0.0018184793875361423 5 0.007273917550144569 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0036369587750722846 18-19 0.0018184793875361423 20-21 0.0027277190813042133 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.009092396937680712 34-35 9.092396937680711E-4 36-37 0.0018184793875361423 38-39 0.012729355712752995 40-41 0.0 42-43 0.005455438162608427 44-45 0.0036369587750722846 46-47 0.0 48-49 0.004546198468840356 50-51 0.0 52-53 9.092396937680711E-4 54-55 0.0 56-57 0.007273917550144569 58-59 0.0027277190813042133 60-61 0.004546198468840356 62-63 9.092396937680711E-4 64-65 0.0036369587750722846 66-67 0.0018184793875361423 68-69 9.092396937680711E-4 70-71 0.0036369587750722846 72-73 0.0036369587750722846 74-75 0.0063646778563764975 76-77 9.092396937680711E-4 78-79 0.005455438162608427 80-81 0.0027277190813042133 82-83 0.0018184793875361423 84-85 0.0018184793875361423 86-87 9.092396937680711E-4 88-89 0.0018184793875361423 90-91 0.00818315724391264 92-93 0.0036369587750722846 94-95 0.0 96-97 0.0 98-99 0.0018184793875361423 100-101 0.0018184793875361423 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 54991.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.597916022621884 #Duplication Level Percentage of deduplicated Percentage of total 1 84.88567140460846 17.484679311160008 2 7.0186280568553014 2.891382226182466 3 2.586739648627174 1.5984433816442691 4 1.3066125187604838 1.0765397974213962 5 0.7504193519908184 0.7728537397028605 6 0.4767370000882847 0.5891873215617102 7 0.27368235190253376 0.39461002709534293 8 0.22954003707954446 0.3782437126075176 9 0.19422618522115298 0.3600589187321562 >10 1.6509225743798004 6.655634558382281 >50 0.21188311115034872 3.0732301649360805 >100 0.22954003707954446 10.574457638522668 >500 0.07945616668138077 12.0201487516139 >1k 0.09711309261057649 32.176174283064505 >5k 0.008828462964597864 9.954356167372842 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5474 9.954356167372842 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2916 5.302685894055391 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2334 4.244330890509356 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1766 3.211434598388827 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1534 2.789547380480442 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1483 2.696804931716099 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1453 2.6422505500900146 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1444 2.6258842356021894 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1333 2.4240330235856775 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1167 2.122165445254678 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1141 2.074884981178738 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1123 2.0421523522030878 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 963 1.751195650197305 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 961 1.7475586914222327 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 831 1.5111563710425342 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 812 1.4766052626793476 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 744 1.3529486643268898 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 596 1.0838137149715408 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 572 1.0401702096706733 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 1.0292593333454565 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 565 1.0274408539579205 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 500 0.9092396937680711 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 447 0.8128602862286557 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 417 0.7583059046025713 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 361 0.6564710589005474 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 352 0.6401047444127221 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 286 0.5200851048353367 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 272 0.49462639340983067 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 266 0.48371551708461386 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 244 0.4437089705588187 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 240 0.43643505300867413 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 225 0.409157862195632 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 212 0.38551763015766216 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 205 0.3727882744449092 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 195 0.3546034805695477 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 194 0.3527850011820116 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 170 0.3091414958811442 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 147 0.26731646996781294 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 147 0.26731646996781294 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 135 0.2454947173173792 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 131 0.23822079976723465 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 118 0.2145805677292648 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 117 0.21276208834172866 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 116 0.2109436089541925 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 107 0.19457729446636723 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 106 0.19275881507883108 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 105 0.19094033569129493 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 100 0.18184793875361424 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 96 0.17457402120346965 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.1673001036533251 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 92 0.1673001036533251 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 88 0.16002618610318053 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 87 0.15820770671564438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 80 0.1454783510028914 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 78 0.1418413922278191 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 77 0.14002291284028295 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 74 0.13456747467767452 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 66 0.1200196395773854 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 65 0.11820116018984923 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 63 0.11456420141477697 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 62 0.11274572202724083 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 62 0.11274572202724083 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 61 0.11092724263970467 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 60 0.10910876325216853 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.1072902838646324 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 58 0.10547180447709625 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 57 0.1036533250895601 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 56 0.10183484570202396 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0027277190813042133 0.0 22-23 0.0 0.0 0.0 0.004546198468840356 0.0 24-25 0.0 0.0 0.0 0.007273917550144569 0.0 26-27 0.0 0.0 0.0 0.011820116018984925 0.0 28-29 0.0 0.0 0.0 0.0345511083631867 0.0 30-31 0.0 0.0 0.0 0.06728373733883726 0.0 32-33 0.0 0.0 0.0 0.10183484570202397 0.0 34-35 0.0 0.0 0.0 0.1382044334527468 0.0 36-37 0.0 0.0 0.0 0.17821097997854193 0.0 38-39 0.0 0.0 0.0 0.21276208834172866 0.0 40-41 0.0 0.0 0.0 0.27277190813042135 0.0 42-43 0.0 0.0 0.0 0.3527850011820116 0.0 44-45 0.0 0.0 0.0 0.5209943445291048 0.0 46-47 0.0 0.0 0.0 0.8655961884672037 0.0 48-49 0.0 0.0 0.0 1.0274408539579203 0.0 50-51 0.0 0.0 0.0 1.2338382644432726 0.0 52-53 0.0 0.0 0.0 1.445691113091233 0.0 54-55 0.0 0.0 0.0 1.7584695677474498 0.0 56-57 0.0 0.0 0.0 2.06942954301613 0.0 58-59 0.0 0.0 0.0 2.309468822170901 0.0 60-61 0.0 0.0 0.0 2.594060846320307 0.0 62-63 0.0 0.0 0.0 2.88956374679493 0.0 64-65 0.0 0.0 0.0 3.1150551908494117 0.0 66-67 0.0 0.0 0.0 3.5087559782509867 0.0 68-69 0.0 0.0 0.0 4.06793838991835 0.0 70-71 0.0 0.0 0.0 4.383444563655871 0.0 72-73 0.0 0.0 0.0 4.826244294520922 0.0 74-75 0.0 0.0 0.0 5.337237002418577 0.0 76-77 0.0 0.0 0.0 5.5499990907603065 0.0 78-79 0.0 0.0 0.0 6.040988525395065 0.0 80-81 0.0 0.0 0.0 6.441053990653016 0.0 82-83 0.0 0.0 0.0 6.835664017748359 0.0 84-85 0.0 0.0 0.0 7.5230492262370205 0.0 86-87 0.0 0.0 0.0 8.355912785728574 0.0 88-89 0.0 0.0 0.0 9.416086268662145 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCAGT 20 0.00206355 71.25 7 TGGGCAG 20 0.00206355 71.25 6 ATGGGGG 25 0.004995935 57.0 5 ATGGGCA 25 0.004995935 57.0 5 CACTGTC 25 0.0016094634 38.0 86-87 CATGGGG 100 2.9418516E-8 38.0 4 GCAGAGA 160 0.0 34.140625 94-95 AAGCAGG 45 2.393307E-5 31.666668 94-95 GTAAGCC 85 1.05501385E-10 30.735296 94-95 AGTACTT 465 0.0 28.091396 12-13 GTAAGCG 45 8.8959484E-4 26.388891 94-95 TACTTTT 525 0.0 24.880953 14-15 TACATGG 525 0.0 23.523811 2 ACATGGG 525 0.0 22.61905 3 GTACATG 550 0.0 22.454546 1 CATGGGC 150 2.9659855E-5 22.166668 4 GAGTACT 515 0.0 21.213593 12-13 GTACTTT 515 0.0 20.291262 14-15 ACTTTTT 530 0.0 19.716982 16-17 GGCTTGA 125 0.0059599513 19.0 1 >>END_MODULE