##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936601_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 56445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.549047745593054 31.0 31.0 34.0 30.0 34.0 2 31.566693241208256 31.0 31.0 34.0 30.0 34.0 3 31.65867658782886 31.0 31.0 34.0 30.0 34.0 4 35.38593320931881 37.0 35.0 37.0 33.0 37.0 5 35.157693329790064 35.0 35.0 37.0 32.0 37.0 6 35.40706882806271 37.0 35.0 37.0 33.0 37.0 7 35.3550535919922 37.0 35.0 37.0 33.0 37.0 8 35.43114536274249 37.0 35.0 37.0 33.0 37.0 9 36.84436176809283 39.0 37.0 39.0 32.0 39.0 10-11 36.70715740986802 38.5 36.0 39.0 32.0 39.0 12-13 36.69139870670564 38.5 35.0 39.0 32.0 39.0 14-15 37.27492249092036 39.0 36.0 40.0 32.0 41.0 16-17 37.36175037647267 39.0 36.0 40.0 32.0 41.0 18-19 37.803773584905656 39.0 37.0 41.0 32.5 41.0 20-21 37.92276552396138 39.5 37.0 41.0 33.0 41.0 22-23 37.84896802196829 39.5 37.0 41.0 32.5 41.0 24-25 37.560102754894146 39.0 36.5 41.0 32.0 41.0 26-27 37.13266011161308 39.0 36.0 40.5 31.5 41.0 28-29 37.11587385950926 39.0 36.0 40.0 31.5 41.0 30-31 37.231738860837986 39.0 36.0 40.5 32.0 41.0 32-33 36.82672513065816 38.0 35.0 40.0 31.0 41.0 34-35 36.37051111701656 38.0 35.0 40.0 30.0 41.0 36-37 35.89146957214988 38.0 35.0 40.0 30.0 41.0 38-39 35.608991053237666 38.0 35.0 40.0 30.0 41.0 40-41 35.59387899725397 38.0 35.0 40.0 30.0 41.0 42-43 35.0464700150589 38.0 35.0 40.0 27.0 41.0 44-45 34.58692532553813 38.0 34.0 40.0 24.5 41.0 46-47 33.85036761449199 38.0 33.0 40.0 21.5 41.0 48-49 33.01345557622464 38.0 33.0 40.0 10.5 41.0 50-51 32.468181415537245 37.0 33.0 40.0 2.0 41.0 52-53 32.34642572415626 37.0 32.5 40.0 2.0 41.0 54-55 32.12936486845602 37.5 31.5 40.0 2.0 41.0 56-57 31.421932854991585 36.5 31.0 40.0 2.0 41.0 58-59 31.040224997785455 36.0 31.0 39.0 2.0 40.0 60-61 30.170661706085568 36.0 28.0 39.0 2.0 40.0 62-63 29.94898573832935 35.0 28.5 39.0 2.0 40.0 64-65 29.52400566923554 35.0 28.0 38.0 2.0 39.5 66-67 28.69715652404996 34.5 26.0 37.0 2.0 39.0 68-69 28.244680662591904 34.0 26.0 37.0 2.0 39.0 70-71 27.379759057489593 33.5 25.5 36.0 2.0 38.0 72-73 26.722685800336613 33.0 25.0 35.0 2.0 37.0 74-75 26.365577110461512 33.0 25.0 35.0 2.0 37.0 76-77 25.028957392151653 30.5 22.5 34.0 2.0 35.0 78-79 25.794206749933565 32.0 24.0 34.0 2.0 35.5 80-81 25.378120294091595 32.0 20.5 34.0 2.0 35.0 82-83 25.028319603153513 31.5 20.0 34.0 2.0 35.0 84-85 24.62455487642838 31.0 18.0 34.0 2.0 35.0 86-87 24.732571529807778 32.0 18.0 34.0 2.0 35.0 88-89 24.285144831251664 31.0 12.5 34.0 2.0 35.0 90-91 23.646647178669504 31.0 4.5 34.0 2.0 35.0 92-93 23.257356718929934 31.0 2.0 34.0 2.0 35.0 94-95 22.58836920896448 30.0 2.0 34.0 2.0 35.0 96-97 21.821197626007617 29.0 2.0 34.0 2.0 35.0 98-99 21.249915847284967 29.0 2.0 34.0 2.0 35.0 100-101 18.5936221100186 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18191343380597047 1101 2 0.4579866325655786 1101 3 0.26846798991600807 1101 4 0.3119411340008469 1101 5 0.2751502858812671 1101 6 0.6078568233074222 1101 7 0.19932503949242886 1101 8 0.45299689621499795 1101 9 0.40197406825934934 1101 10-11 0.22239084920190777 1101 12-13 -0.2716837503914249 1101 14-15 1.0134207512434514 1101 16-17 0.6333106711832457 1101 18-19 0.7249584826625934 1101 20-21 0.6140480499830048 1101 22-23 0.6292060063689817 1101 24-25 0.34062723256306526 1101 26-27 -0.08129643322267555 1101 28-29 -0.0709048438936577 1101 30-31 -0.6794020258437641 1101 32-33 -0.143680284047079 1101 34-35 -1.0087917430755695 1101 36-37 -1.1156003326710788 1101 38-39 -1.3844121226881043 1101 40-41 -2.662676296365852 1101 42-43 -1.846110673225283 1101 44-45 -2.111629737902412 1101 46-47 -3.141339243315503 1101 48-49 -3.1743932308628793 1101 50-51 -2.6334959302972933 1101 52-53 -2.9144663252265275 1101 54-55 -3.4195038082644267 1101 56-57 -3.2485715453140713 1101 58-59 -4.2394174032926735 1101 60-61 -3.8081680808112495 1101 62-63 -4.781128100584226 1101 64-65 -4.044141494972642 1101 66-67 -3.040992562085748 1101 68-69 -4.656633060080516 1101 70-71 -5.103076295717813 1101 72-73 -5.381337691653361 1101 74-75 -4.859273177139887 1101 76-77 -4.053389200284506 1101 78-79 -4.245828903465739 1101 80-81 -3.829868189698967 1101 82-83 -4.253856708070696 1101 84-85 -3.990464868821629 1101 86-87 -4.622316787374686 1101 88-89 -3.913837854328019 1101 90-91 -4.084997734510875 1101 92-93 -5.389546842723835 1101 94-95 -3.768371498751776 1101 96-97 -3.96556636932117 1101 98-99 -3.569273414527583 1101 100-101 -2.670472345885557 1102 1 0.03130105379934989 1102 2 0.16272016450864157 1102 3 0.11752459368959123 1102 4 0.06698913135272022 1102 5 -0.560996685320319 1102 6 -0.4043907768249895 1102 7 -0.8135845930795682 1102 8 -0.15607294820737394 1102 9 -0.08097859642122529 1102 10-11 -0.22911527459816483 1102 12-13 -0.19753611715739794 1102 14-15 -0.338118474178593 1102 16-17 -0.24950958700940618 1102 18-19 -0.08106601253766854 1102 20-21 -0.7404703214171988 1102 22-23 -0.17135672120466694 1102 24-25 -0.32239825568585445 1102 26-27 0.03389721126590217 1102 28-29 -0.8236357277069644 1102 30-31 -0.13372178751209418 1102 32-33 0.05211580996152776 1102 34-35 -0.5667195815396511 1102 36-37 -0.5325152615025885 1102 38-39 -0.7627636619135245 1102 40-41 -1.1835303182129238 1102 42-43 -0.3600133544566617 1102 44-45 -0.8772702542877155 1102 46-47 0.6060953809810883 1102 48-49 0.07751011240094385 1102 50-51 -0.31274121331616556 1102 52-53 -0.05084500778196599 1102 54-55 -0.26971896880729673 1102 56-57 0.021868706258253212 1102 58-59 -0.7826150199758928 1102 60-61 0.4200676027372445 1102 62-63 0.7741516081214996 1102 64-65 1.3692977635510246 1102 66-67 0.049374865918224486 1102 68-69 0.2741845499823725 1102 70-71 1.0870925225542862 1102 72-73 2.0067788260560064 1102 74-75 1.596039633187818 1102 76-77 0.810232117160048 1102 78-79 1.3058096268222457 1102 80-81 1.041200992690971 1102 82-83 1.739357459423509 1102 84-85 2.4072511192617903 1102 86-87 1.9897322030258415 1102 88-89 1.0745765779791618 1102 90-91 1.5338370883954475 1102 92-93 1.2812575266902648 1102 94-95 1.3797582596063869 1102 96-97 0.9978563383915073 1102 98-99 0.23128931304606937 1102 100-101 -0.6416739347634142 1103 1 -0.32247253110631036 1103 2 -0.1475898085371803 1103 3 -0.7315320100839919 1103 4 -0.29958768805428804 1103 5 -1.4316166314119627 1103 6 -0.765576760652479 1103 7 -0.5760509003571954 1103 8 -0.42325623661707823 1103 9 -0.23461741043739437 1103 10-11 -0.6433672961615002 1103 12-13 -0.2719970335994475 1103 14-15 -1.2036845119144388 1103 16-17 -0.6961066220498395 1103 18-19 -0.4702169559338998 1103 20-21 -0.43858352896436514 1103 22-23 -0.7186949961372804 1103 24-25 -1.2403564767101187 1103 26-27 -1.5629693655534993 1103 28-29 -1.5704349190816345 1103 30-31 -0.8739508980242192 1103 32-33 -0.9611928153753979 1103 34-35 -1.5805335977121615 1103 36-37 -0.8146918113678154 1103 38-39 -0.10339708509412304 1103 40-41 -1.341717649749306 1103 42-43 -2.01011443262378 1103 44-45 -1.2079643243685823 1103 46-47 -2.839490872388186 1103 48-49 -2.8871125291084923 1103 50-51 -2.499567358868724 1103 52-53 -2.428877352795446 1103 54-55 -1.6898672167857285 1103 56-57 -3.2247620215045494 1103 58-59 -2.3099061250971857 1103 60-61 -2.4305051735430787 1103 62-63 -1.477869955220818 1103 64-65 -1.8525688132683804 1103 66-67 -1.3276466974240932 1103 68-69 -1.9185378219852822 1103 70-71 -1.604955994965927 1103 72-73 -0.7470958370167686 1103 74-75 -0.2582393425534235 1103 76-77 -0.5945859421391404 1103 78-79 -0.9032537154958113 1103 80-81 -0.5858205706513466 1103 82-83 0.27355557263105723 1103 84-85 0.31122686050168014 1103 86-87 -1.1516087673245607 1103 88-89 -1.4478290823981972 1103 90-91 -0.8827734237339335 1103 92-93 -0.26877616603210797 1103 94-95 -1.4989479398545313 1103 96-97 -0.2219886750855835 1103 98-99 -0.5517295548784595 1103 100-101 -0.5431227218254087 1104 1 0.2457092870755062 1104 2 0.09754006159268869 1104 3 0.12301344446146345 1104 4 -0.11517210363870589 1104 5 0.854097654302322 1104 6 0.5019557068799898 1104 7 0.6385594892531898 1104 8 0.7777177893569487 1104 9 0.5238241480041665 1104 10-11 0.6684833531891456 1104 12-13 0.9640419274395171 1104 14-15 0.7349518517219238 1104 16-17 0.41704272859951885 1104 18-19 -0.07248968320662641 1104 20-21 -0.47494716532799686 1104 22-23 -0.07015603509832147 1104 24-25 -0.41519413904777736 1104 26-27 -0.2348849499690857 1104 28-29 0.457324821178112 1104 30-31 0.3971529980796831 1104 32-33 0.8824760157934364 1104 34-35 0.8542066620280977 1104 36-37 0.578977019801016 1104 38-39 0.8151094084123756 1104 40-41 1.5545485840169206 1104 42-43 1.3290089439995967 1104 44-45 1.646743467839208 1104 46-47 1.6006065302092125 1104 48-49 1.1378874708915063 1104 50-51 2.0665040697027095 1104 52-53 2.738005764087667 1104 54-55 2.5663016143831 1104 56-57 2.390822394079862 1104 58-59 2.8897691995781436 1104 60-61 3.2699818394439397 1104 62-63 3.109143031792172 1104 64-65 3.064151965952398 1104 66-67 2.8025481077707113 1104 68-69 3.2463972429497865 1104 70-71 3.174914134904199 1104 72-73 3.464754812333883 1104 74-75 4.773741176927096 1104 76-77 4.053628343575145 1104 78-79 3.8881423884002935 1104 80-81 4.630737870907094 1104 82-83 4.892873754449237 1104 84-85 3.8329801072549294 1104 86-87 4.7543977261819315 1104 88-89 5.2896709176018035 1104 90-91 4.663168134707625 1104 92-93 4.37456798981205 1104 94-95 5.189363748457158 1104 96-97 5.430446389849486 1104 98-99 5.539179536030634 1104 100-101 5.897948706746025 1105 1 -0.08094710737749722 1105 2 -0.08735978432410718 1105 3 0.26846798991600807 1105 4 0.18467381315636544 1105 5 -0.1967498033247992 1105 6 0.13030701658723132 1105 7 -0.8711477526574427 1105 8 -0.07004800417156076 1105 9 -0.8472082094688744 1105 10-11 -0.2782038579048418 1105 12-13 -0.08464836531857145 1105 14-15 0.28148497961098684 1105 16-17 -0.15660904335734216 1105 18-19 -0.4299627186457613 1105 20-21 -0.1843462408287735 1105 22-23 -0.922236158364889 1105 24-25 -0.3192003023759753 1105 26-27 -0.5398156125268656 1105 28-29 -0.3938308028588722 1105 30-31 -0.4880550142469744 1105 32-33 -0.38840225847467735 1105 34-35 -0.7539597478332283 1105 36-37 -0.26308769514506025 1105 38-39 -1.2398982957478708 1105 40-41 -1.4583944051972537 1105 42-43 -2.1619001469094954 1105 44-45 -1.6151979805429164 1105 46-47 -0.9855259813470241 1105 48-49 -0.6010955799559525 1105 50-51 -0.5741738963989889 1105 52-53 -1.8786352220448421 1105 54-55 -1.736780049715513 1105 56-57 -2.3276675905118154 1105 58-59 -2.1988286432569915 1105 60-61 -1.6776298708796382 1105 62-63 -1.8229062748334073 1105 64-65 -1.6281438738010685 1105 66-67 -2.776496576358717 1105 68-69 -1.5323392747697824 1105 70-71 -1.785948731043412 1105 72-73 -2.068373076726214 1105 74-75 -2.135811981778602 1105 76-77 -3.314168266594347 1105 78-79 -2.252073328086613 1105 80-81 -3.1745009580605448 1105 82-83 -3.271995274826569 1105 84-85 -1.8513034058421418 1105 86-87 -2.5412879440800076 1105 88-89 -2.4171087434151453 1105 90-91 -1.9604065956467664 1105 92-93 -0.7374505001725424 1105 94-95 -2.0764297800482368 1105 96-97 -1.478798602446357 1105 98-99 -2.517831249793712 1105 100-101 -3.1834072254573673 1106 1 0.3108608022270225 1106 2 0.5702673343199649 1106 3 0.41846798991600664 1106 4 0.2575551690885689 1106 5 0.6374309876356534 1106 6 0.10347085839514136 1106 7 0.4870443377380411 1106 8 0.6398390014781583 1106 9 0.2677635419435589 1106 10-11 0.8048469895527788 1106 12-13 0.7458601092576984 1106 14-15 0.6629821547522283 1106 16-17 0.6473457589025386 1106 18-19 0.6381163773994345 1106 20-21 0.8114164710356349 1106 22-23 0.8116621467198613 1106 24-25 0.7090482851946405 1106 26-27 0.9257211106369709 1106 28-29 0.3277793666326545 1106 30-31 0.44866814959483037 1106 32-33 0.9203548036722182 1106 34-35 0.8504187832402224 1106 36-37 1.1668558076798021 1106 38-39 0.5287457720487438 1106 40-41 0.32197282644116143 1106 42-43 1.1547665197571675 1106 44-45 2.3497737708695112 1106 46-47 1.0634853180880057 1106 48-49 -0.06211252910848941 1106 50-51 -0.24182926363062762 1106 52-53 -0.23244878136687674 1106 54-55 -0.22081959773810667 1106 56-57 0.12642845468592512 1106 58-59 -0.6087156489067098 1106 60-61 -0.09062422116212687 1106 62-63 -1.7385842409351042 1106 64-65 -1.1972116704112352 1106 66-67 0.07830568352828848 1106 68-69 0.46003360658614767 1106 70-71 0.13552019551026007 1106 72-73 0.4261184486975189 1106 74-75 0.5744987526846757 1106 76-77 0.39529501024181 1106 78-79 0.4032939035518055 1106 80-81 0.4784651436343701 1106 82-83 0.4693889059643901 1106 84-85 -0.07686837759355569 1106 86-87 0.11803408981829833 1106 88-89 0.6230042509351357 1106 90-91 1.3987741953136847 1106 92-93 2.780628595872656 1106 94-95 2.1189092030026124 1106 96-97 2.65241608681918 1106 98-99 2.9316037784548747 1106 100-101 3.764615373412692 1107 1 -0.26738778534359753 1107 2 0.1160300461843704 1107 3 -0.002718450761957314 1107 4 -0.205156695318216 1107 5 0.1422332475226611 1107 6 -0.12393027154836034 1107 7 0.11190309480019067 1107 8 -0.2395395295952909 1107 9 -0.13534380268921353 1107 10-11 -0.1934580951929732 1107 12-13 -0.19481785684399 1107 14-15 0.4594510813059003 1107 16-17 -0.09728700945904478 1107 18-19 -0.6926745830525434 1107 20-21 -0.21824454591351383 1107 22-23 0.01844180773680648 1107 24-25 -0.1836070820369926 1107 26-27 -0.2432054430353432 1107 28-29 0.24176241748011762 1107 30-31 -0.7083939972978257 1107 32-33 -0.8884022584746774 1107 34-35 -1.1098919512230552 1107 36-37 -1.2630876951450603 1107 38-39 -0.6212542279512618 1107 40-41 -2.1024622018074197 1107 42-43 -2.077154384197634 1107 44-45 -3.1236725568140997 1107 46-47 -2.502475133889398 1107 48-49 -2.5502481223288314 1107 50-51 -2.5063772862294975 1107 52-53 -2.0396521711973854 1107 54-55 -2.355424117512122 1107 56-57 -2.1327523362745247 1107 58-59 -2.0378116941044517 1107 60-61 -2.033562074269472 1107 62-63 -1.5686689866978156 1107 64-65 -0.8739065856654733 1107 66-67 -1.869716915341769 1107 68-69 -0.9814918171426648 1107 70-71 -0.4215419513823946 1107 72-73 -0.6954917207940099 1107 74-75 -0.7036085919480968 1107 76-77 -0.41586318184858584 1107 78-79 -0.1842767179171183 1107 80-81 -0.3185687546707179 1107 82-83 -1.6279274782163995 1107 84-85 -1.4106254397404463 1107 86-87 -0.9819659101817031 1107 88-89 -1.0611765400253184 1107 90-91 -0.8163387990365969 1107 92-93 -0.7543996527149126 1107 94-95 -0.8052433393702678 1107 96-97 -0.26693419566669263 1107 98-99 -0.7551193853869336 1107 100-101 0.3081181982714476 1108 1 -1.0724725311063104 1108 2 -0.9297326656800387 1108 3 -1.064865343417324 1108 4 -0.35911149757809824 1108 5 -0.7292356790310137 1108 6 -0.3631958082715272 1108 7 -0.8629556622619603 1108 8 -0.39349433185517313 1108 9 -1.2489031247231068 1108 10-11 -0.7868196771138827 1108 12-13 -0.8624732240756359 1108 14-15 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2.944370547556467 2316 76-77 2.859397574344378 2316 78-79 2.0699605702184734 2316 80-81 1.6765420667112885 2316 82-83 2.470670957246444 2316 84-85 2.230182904457724 2316 86-87 1.931495628279837 2316 88-89 2.8262093791402627 2316 90-91 1.8885177850572745 2316 92-93 0.6742183394623993 2316 94-95 1.182370741464151 2316 96-97 0.1729289073320004 2316 98-99 0.28480890666000036 2316 100-101 -0.8212820624847446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 3.0 6 13.0 7 16.0 8 39.0 9 57.0 10 131.0 11 212.0 12 337.0 13 478.0 14 646.0 15 862.0 16 1154.0 17 1509.0 18 1231.0 19 820.0 20 723.0 21 778.0 22 736.0 23 767.0 24 761.0 25 843.0 26 922.0 27 1118.0 28 1292.0 29 1559.0 30 1941.0 31 2287.0 32 2804.0 33 3364.0 34 4463.0 35 5866.0 36 8303.0 37 8072.0 38 2329.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.7113970262463 18.661279174863097 14.331791518244813 12.29553228064579 2 14.636523094913272 29.728388937120176 27.37194592583406 28.263142042132493 3 36.34060346202229 22.96025938590741 30.775500079729273 9.923637072341029 4 12.342988750110727 16.768535742758438 41.03109221365931 29.857383293471525 5 8.528958418227237 45.217298867884416 32.7888312102475 13.464911503640842 6 24.81885020816724 36.93506953671716 11.898308087518823 26.347772167596776 7 19.633271326069625 38.46576313225263 21.69368411728231 20.20728142439543 8 24.689520772433344 19.004340508459563 18.715563823190717 37.590574895916376 9 16.45495615200638 27.55248471963859 29.09380813180973 26.898750996545314 10-11 27.139693506953673 22.678713792187086 22.249092036495703 27.93250066436354 12-13 37.183098591549296 22.61227743821419 22.151652050668794 18.05297191956772 14-15 25.890492262173677 27.16876168203602 33.691213337231034 13.249532718559268 16-17 16.64703016344067 29.17305222128715 32.793550958940514 21.386366656331663 18-19 15.6940384445035 35.798564974754186 29.80334839224024 18.70404818850208 20-21 21.893878997253964 24.515014615997874 26.04127912126849 27.54982726547967 22-23 17.434402848891803 25.490317665609552 31.56458728274543 25.510692202753223 24-25 27.85479541850845 25.904206787198042 26.870642843855467 19.370354950438042 26-27 23.34056783452186 19.358639323205033 40.17894317225495 17.12184967001816 28-29 15.784018426647767 36.57158043940468 29.94507441530829 17.699326718639263 30-31 19.032686686154666 26.283107449729826 28.78908672158739 25.895119142528124 32-33 20.357870493400657 27.852777039596067 32.55735671892993 19.231995748073345 34-35 25.85800598876663 24.622158436542108 29.560233172097313 19.95960240259395 36-37 20.750086384860055 23.015584714752762 38.42664374883712 17.807685151550054 38-39 19.88199440093554 28.693433502250258 33.94610014529218 17.478471951522025 40-41 19.009274761487152 31.489011135028832 30.332102013517943 19.169612089966073 42-43 17.42935601027549 31.816812826645407 23.890512888652673 26.863318274426433 44-45 22.53935351280484 23.36141451185699 27.441600892928324 26.657631082409843 46-47 28.01222428913101 30.22765523961378 28.31960315351227 13.440517317742936 48-49 24.210293205775535 26.846487731419966 29.728053857737617 19.215165205066878 50-51 13.141343632627029 30.893451916944226 37.14389483381758 18.821309616611153 52-53 21.05505603047349 34.723833990344154 22.31828852371883 21.902821455463524 54-55 16.919724323653952 25.815424410467198 28.455255744733627 28.809595521145226 56-57 25.223006670269026 23.69584281905234 25.510900087696765 25.570250422981864 58-59 31.86343986960527 23.1583516405641 24.697930692367656 20.280277797462972 60-61 21.691293717444456 27.936642925481024 34.209453952730236 16.162609404344284 62-63 17.178723932032813 28.220733889686212 32.584737504208086 22.015804674072893 64-65 16.130546868770985 36.246777522834186 26.780889270812107 20.84178633758272 66-67 21.150353893716726 25.748757607164684 23.050484112431015 30.050404386687575 68-69 17.500265759540767 26.4962262145211 28.696715212076114 27.306792813862018 70-71 27.044910975285674 26.133404198777573 23.77447072371335 23.047214102223403 72-73 22.3522429310467 19.78421089929842 36.126957692580255 21.736588477074623 74-75 16.35321527599373 35.394169184022395 26.137682379100486 22.114933160883393 76-77 19.467944686090906 24.596263387755464 25.808137629226724 30.12765429692691 78-79 20.866037100689216 25.541716128346415 29.449336475257347 24.142910295707022 80-81 26.001913468693527 23.254845682293325 26.103787959321075 24.639452889692073 82-83 20.30420878254476 21.946617294012384 34.17255033973229 23.576623583710568 84-85 19.204840409982015 27.253882338350326 29.433129877838116 24.108147373829542 86-87 19.022730896656803 28.817568166114487 25.910210300657297 26.24949063657141 88-89 17.75440958902896 28.1327793477972 21.168685052135473 32.944126011038364 90-91 21.883195123679922 20.217591608193352 25.12757814161174 32.771635126514994 92-93 25.9168379278577 26.84430586067607 26.22953724045071 21.00931897101552 94-95 21.975072417550294 24.218909174661388 27.79682337204461 26.00919503574371 96-97 12.115440831259026 27.181567734677426 34.17516321342203 26.527828220641513 98-99 19.385043318037667 30.001063019329234 21.8326453236008 28.7812483390323 100-101 16.354697258271692 23.49027771625991 25.705806794525405 34.449218230942996 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 96.0 1 74.5 2 58.0 3 60.0 4 56.5 5 55.0 6 77.5 7 113.0 8 138.0 9 159.0 10 136.5 11 93.0 12 83.0 13 85.5 14 80.5 15 72.5 16 75.5 17 98.0 18 110.0 19 99.5 20 97.5 21 126.0 22 141.0 23 152.0 24 185.5 25 208.0 26 254.5 27 301.0 28 339.0 29 405.0 30 458.5 31 531.5 32 613.5 33 630.0 34 621.0 35 650.5 36 636.0 37 583.5 38 522.0 39 446.5 40 378.0 41 323.5 42 279.0 43 243.5 44 236.0 45 344.5 46 1130.0 47 5963.0 48 14762.5 49 13826.0 50 5457.0 51 1851.5 52 741.5 53 319.5 54 171.0 55 121.5 56 109.0 57 99.0 58 72.0 59 64.0 60 49.0 61 39.0 62 39.5 63 29.5 64 20.5 65 18.0 66 15.0 67 11.5 68 12.0 69 10.5 70 8.5 71 5.5 72 3.0 73 3.0 74 2.0 75 1.0 76 2.5 77 3.0 78 1.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03188944990698911 2 0.007086544423775356 3 0.007086544423775356 4 0.0 5 0.003543272211887678 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0026574541589157587 16-17 0.004429090264859598 18-19 0.0 20-21 0.0 22-23 0.003543272211887678 24-25 8.858180529719195E-4 26-27 0.004429090264859598 28-29 0.008858180529719196 30-31 0.0 32-33 0.0 34-35 0.010629816635663035 36-37 0.02037381521835415 38-39 0.012401452741606873 40-41 0.0026574541589157587 42-43 0.0 44-45 0.0026574541589157587 46-47 0.0 48-49 0.0 50-51 0.001771636105943839 52-53 0.004429090264859598 54-55 0.003543272211887678 56-57 8.858180529719195E-4 58-59 0.001771636105943839 60-61 0.005314908317831517 62-63 0.010629816635663035 64-65 0.009743998582691114 66-67 0.0026574541589157587 68-69 0.005314908317831517 70-71 0.0 72-73 0.001771636105943839 74-75 0.006200726370803437 76-77 0.006200726370803437 78-79 0.007086544423775356 80-81 0.005314908317831517 82-83 0.006200726370803437 84-85 0.006200726370803437 86-87 0.003543272211887678 88-89 0.009743998582691114 90-91 0.015944724953494555 92-93 0.001771636105943839 94-95 0.0026574541589157587 96-97 8.858180529719195E-4 98-99 0.003543272211887678 100-101 0.004429090264859598 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 56445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.214367968819204 #Duplication Level Percentage of deduplicated Percentage of total 1 84.82033304119194 17.145894233324473 2 7.0727432077125325 2.8594206749933564 3 2.532865907099036 1.5360085038533084 4 1.1393514460999123 0.9212507750907964 5 0.7975460122699387 0.8060944282044468 6 0.4557405784399649 0.5527504650544778 7 0.33304119193689746 0.47125520418106126 8 0.29798422436459243 0.48188502081672424 9 0.21034180543382996 0.38267339888386925 >10 1.7177914110429449 6.838515368943219 >50 0.18404907975460122 2.5281247231818584 >100 0.2366345311130587 9.471166622375764 >500 0.09640666082383874 13.937461245460185 >1k 0.09640666082383874 32.553813446718046 >5k 0.008764241893076249 9.513685888918417 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5370 9.513685888918417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3181 5.635574453007353 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2331 4.129683762955089 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1805 3.1978031712286294 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1607 2.8470192222517494 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1561 2.765523961378333 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1520 2.6928868810346356 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1475 2.6131632562671627 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1365 2.4182832846133406 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1232 2.18265568252281 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1205 2.134821507662326 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1093 1.9363982637966162 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 988 1.7503764726725128 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 937 1.6600230312693773 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 872 1.5448666843830279 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 868 1.5377801399592523 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 787 1.3942776153778014 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 632 1.1196740189565064 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 612 1.0842412968376296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 1.0328638497652582 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 582 1.0310922136593144 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 503 0.8911329612897512 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 503 0.8911329612897512 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 459 0.8131809726282222 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.6537337230932767 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 351 0.6218442731862875 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 312 0.5527504650544778 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 306 0.5421206484188148 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 286 0.506687926299938 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 248 0.4393657542740721 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 236 0.418106121002746 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 229 0.40570466826113916 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 224 0.39684648773141995 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 206 0.3649570378244309 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 184 0.3259810434936664 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 174 0.30826468243422805 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 157 0.2781468686331827 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 151 0.26751705199751974 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 149 0.263973779785632 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 144 0.2551155992559129 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 137 0.24271414651430598 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 134 0.23739923819647443 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 127 0.22499778545486757 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 117 0.20728142439542918 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 114 0.20196651607759766 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 113 0.2001948799716538 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 106 0.18779342723004694 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 105 0.1860217911241031 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 105 0.1860217911241031 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 103 0.18247851891221545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.17007706617060855 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 88 0.15590397732305783 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 86 0.15236070511117017 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 83 0.14704579679333865 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 82 0.1452741606873948 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 80 0.14173088847550713 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 73 0.12932943573390027 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 70 0.12401452741606875 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 69 0.12224289131012489 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 68 0.12047125520418106 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 63 0.11161307467446187 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 62 0.10984143856851802 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 61 0.1080698024625742 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 60 0.10629816635663034 No Hit TTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTAC 57 0.10098325803879883 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 57 0.10098325803879883 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.001771636105943839 0.0 24-25 0.0 0.0 0.0 0.009743998582691114 0.0 26-27 0.0 0.0 0.0 0.012401452741606873 0.0 28-29 0.0 0.0 0.0 0.030117813801045265 0.0 30-31 0.0 0.0 0.0 0.06820799007883781 0.0 32-33 0.0 0.0 0.0 0.10186907609177075 0.0 34-35 0.0 0.0 0.0 0.12490034546904066 0.0 36-37 0.0 0.0 0.0 0.16387633979980512 0.0 38-39 0.0 0.0 0.0 0.2081672424484011 0.0 40-41 0.0 0.0 0.0 0.2595446895207724 0.0 42-43 0.0 0.0 0.0 0.31889449906989104 0.0 44-45 0.0 0.0 0.0 0.46505447781025777 0.0 46-47 0.0 0.0 0.0 0.8282398795287448 0.0 48-49 0.0 0.0 0.0 1.0248914872885109 0.0 50-51 0.0 0.0 0.0 1.2277438214190806 0.0 52-53 0.0 0.0 0.0 1.4261670652847904 0.0 54-55 0.0 0.0 0.0 1.7220302949774116 0.0 56-57 0.0 0.0 0.0 1.9851182567100718 0.0 58-59 0.0 0.0 0.0 2.234033129595181 0.0 60-61 0.0 0.0 0.0 2.5245814509699707 0.0 62-63 0.0 0.0 0.0 2.8567632208344405 0.0 64-65 0.0 0.0 0.0 3.0808751882363365 0.0 66-67 0.0 0.0 0.0 3.4706351315439807 0.0 68-69 0.0 0.0 0.0 3.979094693949863 0.0 70-71 0.0 0.0 0.0 4.26432810700682 0.0 72-73 0.0 0.0 0.0 4.669146957214988 0.0 74-75 0.0 0.0 0.0 5.045619629728054 0.0 76-77 0.0 0.0 0.0 5.279475595712641 0.0 78-79 0.0 0.0 0.0 5.768447160953141 0.0 80-81 0.0 0.0 0.0 6.147577287625122 0.0 82-83 0.0 0.0 0.0 6.5160775976614405 0.0 84-85 0.0 0.0 0.0 7.145008415271503 0.0 86-87 0.0 0.0 0.0 7.972362476747276 0.0 88-89 0.0 0.0 0.0 9.057489591637879 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGAGA 115 0.0 35.10706 94-95 AAGCAGG 40 4.4694977E-4 29.686115 94-95 CTTGATA 105 0.0021600167 22.638062 2 GCTTGAT 110 0.0028300774 21.609058 1 GAGTACT 575 0.0 20.65121 12-13 AGTACAT 380 0.0 20.016811 2 CCCTGTC 60 0.0047386778 19.790745 90-91 GTACTTT 570 0.0 19.582418 14-15 ACTTTTT 590 0.0 18.91861 16-17 GTACATG 420 1.8189894E-12 18.110449 3 TACATGG 420 1.8189894E-12 18.110449 4 CATGGGA 250 4.4091794E-6 17.099201 6 AACGCCT 85 0.0017746694 16.763924 86-87 CGCCTGT 85 0.0017746694 16.763924 88-89 ACATGGG 430 6.184564E-11 16.583694 5 AGTACTT 520 0.0 16.44154 12-13 ACGCCTG 90 0.0025998452 15.832595 88-89 CAACGCC 90 0.0025998452 15.832595 86-87 TACTTTT 585 0.0 14.614701 14-15 CTATCTC 100 0.0052406727 14.249335 94-95 >>END_MODULE