##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936600_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 57645 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.568496834070604 31.0 31.0 34.0 30.0 34.0 2 31.61368722352329 31.0 31.0 34.0 30.0 34.0 3 31.687188828172435 31.0 31.0 34.0 30.0 34.0 4 35.3714459189869 37.0 35.0 37.0 33.0 37.0 5 35.18312082574378 37.0 35.0 37.0 33.0 37.0 6 35.424130453638654 37.0 35.0 37.0 33.0 37.0 7 35.39293954375922 37.0 35.0 37.0 33.0 37.0 8 35.440419810911614 37.0 35.0 37.0 33.0 37.0 9 36.87624251886547 39.0 37.0 39.0 33.0 39.0 10-11 36.72267325873884 38.5 36.0 39.0 32.5 39.0 12-13 36.72334981351375 39.0 35.0 39.0 32.0 39.0 14-15 37.32099054558071 39.0 36.0 40.0 32.0 41.0 16-17 37.38494231936855 39.0 36.0 40.0 32.0 41.0 18-19 37.842518865469685 39.0 37.0 41.0 32.5 41.0 20-21 37.94176424668228 39.5 37.0 41.0 33.0 41.0 22-23 37.86860091942059 39.5 37.0 41.0 32.5 41.0 24-25 37.54963136438546 39.0 36.5 41.0 32.0 41.0 26-27 37.16396044756701 39.0 36.0 40.5 31.5 41.0 28-29 37.143126030011274 39.0 36.0 40.0 31.5 41.0 30-31 37.237080405932865 39.0 36.0 40.5 32.0 41.0 32-33 36.82295949345129 38.0 35.0 40.0 31.0 41.0 34-35 36.346508803885854 38.0 35.0 40.0 30.0 41.0 36-37 35.993763552779946 38.0 35.0 40.0 30.0 41.0 38-39 35.70085870413739 38.0 35.0 40.0 30.0 41.0 40-41 35.71245554688177 38.0 35.0 40.0 30.0 41.0 42-43 35.18192384421893 38.0 35.0 40.0 28.0 41.0 44-45 34.646734322144155 38.0 34.0 40.0 24.5 41.0 46-47 33.89371151010495 38.0 33.0 40.0 21.5 41.0 48-49 33.02737444704658 37.5 33.0 40.0 10.5 41.0 50-51 32.52254315205135 37.0 33.0 40.0 4.0 41.0 52-53 32.361280249804835 37.5 32.0 40.0 2.0 41.0 54-55 32.216298031052126 38.0 31.5 40.0 2.0 41.0 56-57 31.549926272877094 36.5 31.0 40.0 2.0 41.0 58-59 31.1232110330471 36.0 31.0 39.0 2.0 40.0 60-61 30.223679417122042 36.0 28.0 39.0 2.0 40.0 62-63 30.008422239569782 35.0 28.5 39.0 2.0 40.0 64-65 29.62356665799289 35.0 28.0 38.0 2.0 39.5 66-67 28.83430479659988 34.5 27.0 37.0 2.0 39.0 68-69 28.306904328215804 34.0 26.0 37.0 2.0 39.0 70-71 27.49350333940498 33.5 26.0 36.0 2.0 38.0 72-73 26.837314597970334 33.0 25.0 35.0 2.0 37.0 74-75 26.506548703270013 33.0 25.5 35.0 2.0 37.0 76-77 25.085367334547662 30.5 22.5 34.0 2.0 35.0 78-79 25.817763899731112 32.0 24.0 34.0 2.0 35.5 80-81 25.392254315205136 32.0 20.5 34.0 2.0 35.0 82-83 25.07592158903634 31.5 20.0 34.0 2.0 35.0 84-85 24.69548096105473 31.0 19.5 34.0 2.0 35.0 86-87 24.788377135918118 32.0 18.0 34.0 2.0 35.0 88-89 24.305820105820107 31.0 13.0 34.0 2.0 35.0 90-91 23.702801630670482 31.0 6.0 34.0 2.0 35.0 92-93 23.22914389799636 31.0 2.0 34.0 2.0 35.0 94-95 22.544288316419465 30.0 2.0 34.0 2.0 35.0 96-97 21.822942145892966 29.0 2.0 34.0 2.0 35.0 98-99 21.287657212247375 29.5 2.0 34.0 2.0 35.0 100-101 18.633732327174947 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35145306344342586 1101 2 0.2278084475747697 1101 3 0.36020358335478875 1101 4 0.42505955945855334 1101 5 0.44121332627943133 1101 6 0.08952034026798117 1101 7 0.3754837869433558 1101 8 0.5689178339330496 1101 9 0.601850822315086 1101 10-11 0.32998782114839287 1101 12-13 0.9255613296158174 1101 14-15 0.7283954730211306 1101 16-17 0.4328309984089387 1101 18-19 0.49110890287475684 1101 20-21 0.6921502105101638 1101 22-23 0.4733289342939955 1101 24-25 -0.03276677161439778 1101 26-27 0.7916077020065799 1101 28-29 1.102889286354987 1101 30-31 1.05966990580351 1101 32-33 0.8551599889428374 1101 34-35 1.0789551915158242 1101 36-37 0.8997251216575819 1101 38-39 1.4839605768206 1101 40-41 1.9727471444771894 1101 42-43 1.9945534494623942 1101 44-45 2.2689276252341557 1101 46-47 2.2512532936348677 1101 48-49 2.8084786115424336 1101 50-51 2.032378650086031 1101 52-53 2.6859372467591456 1101 54-55 2.8814771746568226 1101 56-57 3.4885983690998614 1101 58-59 2.2230044681649765 1101 60-61 3.2423971815367594 1101 62-63 3.5094886794856457 1101 64-65 3.536625680937348 1101 66-67 3.8871763757923468 1101 68-69 2.406424382900923 1101 70-71 2.3440597174665285 1101 72-73 2.6736531299963104 1101 74-75 2.6917364808269113 1101 76-77 2.194368018014334 1101 78-79 2.899052192896505 1101 80-81 3.4870309303648312 1101 82-83 3.5189245148590125 1101 84-85 3.5806143976665012 1101 86-87 3.3908781728603223 1101 88-89 3.4530655047170953 1101 90-91 3.383369281240615 1101 92-93 2.9853480608244816 1101 94-95 3.362654870909534 1101 96-97 3.8195623677118498 1101 98-99 4.513525198531145 1101 100-101 4.624328895273361 1102 1 0.37343108542144776 1102 2 0.2360502058165288 1102 3 0.11981896797017555 1102 4 0.15445516385415914 1102 5 -0.13845700339089717 1102 6 0.44528957103721467 1102 7 0.10487939133896163 1102 8 0.19254420755942903 1102 9 0.15816950363377202 1102 10-11 0.04427353543410817 1102 12-13 0.3033085823630728 1102 14-15 0.09515371477937862 1102 16-17 -0.012223946646003014 1102 18-19 0.5900100017758589 1102 20-21 0.23335900171895219 1102 22-23 0.4389882749533385 1102 24-25 0.6231398217921935 1102 26-27 0.6460033064021786 1102 28-29 0.03008708855278286 1102 30-31 -0.42384657771297896 1102 32-33 0.7562588900417353 1102 34-35 0.9264826640432986 1102 36-37 0.321428418360874 1102 38-39 0.3177517856118115 1102 40-41 -1.3232693390392996 1102 42-43 -0.304210286801343 1102 44-45 -0.1239295176229831 1102 46-47 0.1070225244040941 1102 48-49 0.6120500401138642 1102 50-51 0.6044940347014176 1102 52-53 0.5602504335723282 1102 54-55 0.5655431087227569 1102 56-57 1.139010457011949 1102 58-59 2.0918231494836483 1102 60-61 2.2787982804378615 1102 62-63 1.1544062619032296 1102 64-65 0.2811311754428374 1102 66-67 0.04583022194619346 1102 68-69 0.9009298774064121 1102 70-71 1.4457080691148825 1102 72-73 1.3117025805457594 1102 74-75 0.4932474698379039 1102 76-77 0.98695043559675 1102 78-79 0.4704807643250746 1102 80-81 1.3324979633318605 1102 82-83 1.6398036357381365 1102 84-85 1.8237462657983698 1102 86-87 1.6621693816515304 1102 88-89 1.4949611091126975 1102 90-91 1.3771879625592973 1102 92-93 1.1948260828025035 1102 94-95 0.7239186071732711 1102 96-97 -0.7099705993211209 1102 98-99 -0.295540735534793 1102 100-101 0.2431475765920439 1103 1 0.1986190553462599 1103 2 0.09757902787166728 1103 3 0.08692423112806935 1103 4 -0.030068645669651062 1103 5 0.6854778337018388 1103 6 0.6053772903354613 1103 7 -0.5533286287111636 1103 8 0.09354671382509139 1103 9 -0.052670095363723135 1103 10-11 -0.2248367402551139 1103 12-13 -0.29474906174720417 1103 14-15 0.33230910325055163 1103 16-17 0.7963600132537465 1103 18-19 0.49414534012171885 1103 20-21 -0.0949618002860575 1103 22-23 0.012296545630029243 1103 24-25 -0.25796920076419383 1103 26-27 -0.624046818911097 1103 28-29 -0.20142920217403315 1103 30-31 -0.28192928447989374 1103 32-33 0.16572004292394382 1103 34-35 -0.5096075615206175 1103 36-37 -0.20520065432082646 1103 38-39 -1.2552432018568638 1103 40-41 -0.4477994142272692 1103 42-43 -1.1925248231421932 1103 44-45 -0.4810723747658443 1103 46-47 0.003952348965498231 1103 48-49 0.5321628220687487 1103 50-51 0.33146771891193794 1103 52-53 1.5030762481086768 1103 54-55 1.7215581463167453 1103 56-57 1.1469991788164577 1103 58-59 1.7132203925914276 1103 60-61 1.2366616889591633 1103 62-63 1.1699137807002202 1103 64-65 1.1877727794528639 1103 66-67 0.5754354851040908 1103 68-69 0.23301007790766803 1103 70-71 -0.21751248226356523 1103 72-73 -0.7774264921359446 1103 74-75 0.23306576557725123 1103 76-77 -0.5735132235511173 1103 78-79 -0.5066495614894606 1103 80-81 -1.1698516607282876 1103 82-83 -1.1001713016052257 1103 84-85 -0.14053944848734545 1103 86-87 0.006937552077594944 1103 88-89 -0.21039603374444482 1103 90-91 0.06218169689513786 1103 92-93 0.8040052807974902 1103 94-95 0.8962243715842959 1103 96-97 0.42145170644329255 1103 98-99 -0.598485597690182 1103 100-101 0.7044571004015658 1104 1 0.1796371744144203 1104 2 0.015324210500370583 1104 3 0.44154027944558294 1104 4 0.538237365259306 1104 5 -0.1905647317281307 1104 6 0.0620928497257367 1104 7 0.0952189697932937 1104 8 -0.17162441070755108 1104 9 -0.9908351802069788 1104 10-11 -0.8605859493434096 1104 12-13 -0.5318787712669106 1104 14-15 -0.4361694702323362 1104 16-17 -0.16064315039307786 1104 18-19 -0.6611609584115001 1104 20-21 -0.7350250732224879 1104 22-23 -0.6939157063112944 1104 24-25 -1.1000744639220912 1104 26-27 -0.2878374430123358 1104 28-29 -0.010603777957747695 1104 30-31 -0.11324938801742945 1104 32-33 -0.5356029366561543 1104 34-35 -0.7749224881815309 1104 36-37 -0.45872380646347466 1104 38-39 -0.6544379099385438 1104 40-41 -0.4296803460650622 1104 42-43 -1.1922372188856514 1104 44-45 -0.22697401411010532 1104 46-47 0.35497334407622105 1104 48-49 0.18142943121456057 1104 50-51 0.7878956740456786 1104 52-53 -0.2642519083480437 1104 54-55 -0.7547145025184605 1104 56-57 -1.684320924721078 1104 58-59 -1.5705012064882418 1104 60-61 -0.7541349748314587 1104 62-63 -2.207566806012462 1104 64-65 -1.2042318222152417 1104 66-67 -1.2994566632997113 1104 68-69 -2.580334759595928 1104 70-71 -1.882453523390975 1104 72-73 -1.4325889885408927 1104 74-75 -1.2387490172815383 1104 76-77 -1.1070191194383767 1104 78-79 -2.3488986267756253 1104 80-81 -1.9079879851458905 1104 82-83 -1.6617186125054246 1104 84-85 -1.517588628815215 1104 86-87 -0.9729301499643945 1104 88-89 -0.7021993124329704 1104 90-91 -1.2339068852158768 1104 92-93 -0.32786127082747285 1104 94-95 -0.9054724935293059 1104 96-97 -0.36644015435624766 1104 98-99 -0.44044705871980483 1104 100-101 1.0295937124234236 1105 1 -0.3005320482191074 1105 2 -0.2697101628470655 1105 3 -0.4648584513846643 1105 4 -0.13759552739008285 1105 5 -0.33546161168582955 1105 6 -0.574871719285369 1105 7 -0.12265517087302413 1105 8 -0.38579680626546065 1105 9 -0.8435586069653098 1105 10-11 -0.262754114335479 1105 12-13 -0.3988803577290909 1105 14-15 -0.32247301333122635 1105 16-17 -0.4471317807473838 1105 18-19 -0.4468563576711517 1105 20-21 -0.4164105835344998 1105 22-23 -0.17276287712039107 1105 24-25 0.22250618123920418 1105 26-27 -0.09362803000334452 1105 28-29 0.09978754938227752 1105 30-31 0.004148813992088662 1105 32-33 -0.02542313760803694 1105 34-35 -0.035498902776986085 1105 36-37 -0.7770738857865851 1105 38-39 -0.7945754033282881 1105 40-41 -0.31664491507616077 1105 42-43 0.15863533531847196 1105 44-45 0.23666956071802758 1105 46-47 -0.2921710239830091 1105 48-49 0.034285063155337525 1105 50-51 0.8968327443788411 1105 52-53 0.4222896040792463 1105 54-55 0.756787348354095 1105 56-57 0.005657922449731956 1105 58-59 -0.5207920578896186 1105 60-61 0.9253996629263384 1105 62-63 0.7076896720414787 1105 64-65 0.6048638943368481 1105 66-67 0.8220398993655458 1105 68-69 0.426275499526227 1105 70-71 0.8195559953821636 1105 72-73 0.6426357602692647 1105 74-75 0.48547097214204626 1105 76-77 0.47600573513592437 1105 78-79 1.7740521928965052 1105 80-81 0.908246811258131 1105 82-83 1.751554787811866 1105 84-85 1.8675250676416866 1105 86-87 2.66072928948563 1105 88-89 2.7089588049652313 1105 90-91 2.3331211422828027 1105 92-93 1.4825564975490444 1105 94-95 1.7469600818276483 1105 96-97 2.86174599054063 1105 98-99 3.234989218382257 1105 100-101 2.9087581756703855 1106 1 0.023180458855030395 1106 2 -0.007684130023069002 1106 3 0.17464352937368588 1106 4 0.1804576701198215 1106 5 0.10653046528078391 1106 6 0.13169307980914624 1106 7 -0.4656093304655542 1106 8 -0.5555760931924567 1106 9 -0.052670095363723135 1106 10-11 0.20498782114839287 1106 12-13 0.4684088329896383 1106 14-15 0.2445898050049422 1106 16-17 -0.39662244288660276 1106 18-19 0.2836190243322534 1106 20-21 -0.05110215116324923 1106 22-23 0.4070333877352965 1106 24-25 0.45255711502527873 1106 26-27 0.6040233565274917 1106 28-29 0.5792725522119326 1106 30-31 0.46368475060781833 1106 32-33 0.262211270994122 1106 34-35 0.7009187542688622 1106 36-37 0.3211151351528585 1106 38-39 0.4552831139326088 1106 40-41 0.8767619892814977 1106 42-43 1.32501903650693 1106 44-45 1.3785767480411693 1106 46-47 0.9951804191409366 1106 48-49 0.7602329975073516 1106 50-51 1.0683098241751026 1106 52-53 -0.330257085224666 1106 54-55 0.04611954982551936 1106 56-57 0.839981634956807 1106 58-59 0.8272554803107148 1106 60-61 -0.13175936367241547 1106 62-63 0.889212026314258 1106 64-65 0.5123341829616379 1106 66-67 -0.8193013570011765 1106 68-69 -0.08277939577654081 1106 70-71 0.3088033072101162 1106 72-73 0.1436261394430005 1106 74-75 -0.12658335722976943 1106 76-77 -0.3366711182879598 1106 78-79 0.4056311402649264 1106 80-81 -0.07336043265811298 1106 82-83 0.7156181720789867 1106 84-85 1.201565814670552 1106 86-87 1.2350077275161908 1106 88-89 0.8860951943257334 1106 90-91 0.834111521456542 1106 92-93 1.3303210702711752 1106 94-95 1.8962243715842995 1106 96-97 2.3425043380222412 1106 98-99 2.226075805818592 1106 100-101 2.8711237670682372 1107 1 -0.6075212955309333 1107 2 -0.19014027037394854 1107 3 -0.3218476986964909 1107 4 0.053264687663677535 1107 5 -0.2075046224385133 1107 6 -0.21304376229611677 1107 7 -0.0963110848515143 1107 8 0.14968706470228454 1107 9 0.006979027443293262 1107 10-11 -0.17001217885160713 1107 12-13 -0.48597713192264536 1107 14-15 0.7130108576365188 1107 16-17 -0.12293823236029056 1107 18-19 -0.5992757125098507 1107 20-21 -0.04759337923341889 1107 22-23 -0.024106963141896642 1107 24-25 -0.3417411305887583 1107 26-27 -0.7801871697882916 1107 28-29 -0.2996748162091194 1107 30-31 -0.051227530093932216 1107 32-33 0.262211270994122 1107 34-35 -0.10566019309955976 1107 36-37 0.8035712755037352 1107 38-39 -0.1346291667691517 1107 40-41 0.22851637524642143 1107 42-43 0.6701944751034219 1107 44-45 0.75226095856749 1107 46-47 0.5403558577374312 1107 48-49 0.9918119448757707 1107 50-51 0.01699403470141192 1107 52-53 0.22632186214375594 1107 54-55 -0.1832664150867629 1107 56-57 0.12770093320241926 1107 58-59 -0.5718673267068262 1107 60-61 -0.47298743384785524 1107 62-63 -1.0616651666682024 1107 64-65 -1.2652973959857334 1107 66-67 -0.5250031113871394 1107 68-69 0.037834639311178364 1107 70-71 0.3921366405434519 1107 72-73 -0.4936545623113844 1107 74-75 -0.678776339685907 1107 76-77 0.09052186416817776 1107 78-79 0.33238552622983875 1107 80-81 0.19856939190329115 1107 82-83 0.12789887383337017 1107 84-85 0.9094605515126517 1107 86-87 0.36395509593724285 1107 88-89 0.39793729958888946 1107 90-91 1.3301641530354864 1107 92-93 1.6347070351834567 1107 94-95 1.1453471786018419 1107 96-97 1.8727674959169782 1107 98-99 2.036602121608066 1107 100-101 2.9127904337349015 1108 1 0.42700251399287836 1108 2 -0.3353783656120406 1108 3 0.012676110827317899 1108 4 0.35088373528272854 1108 5 -0.3170284319623278 1108 6 -0.3761390003913547 1108 7 -0.3058348943753302 1108 8 -0.6645986495834322 1108 9 0.015312360776626122 1108 10-11 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72-73 -4.6931828641981745 2316 74-75 -3.771071937170184 2316 76-77 -3.553817758473329 2316 78-79 -4.178777995782742 2316 80-81 -3.207090985455199 2316 82-83 -3.9632960947200857 2316 84-85 -3.687709259808102 2316 86-87 -3.3747241493457736 2316 88-89 -4.653792260159541 2316 90-91 -5.464961633128034 2316 92-93 -3.9781860465775516 2316 94-95 -3.7188037647943837 2316 96-97 -5.388710491504408 2316 98-99 -5.063397878391935 2316 100-101 -6.273530320982079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 1.0 5 3.0 6 10.0 7 22.0 8 32.0 9 66.0 10 112.0 11 231.0 12 338.0 13 450.0 14 619.0 15 857.0 16 1197.0 17 1482.0 18 1238.0 19 778.0 20 748.0 21 810.0 22 790.0 23 701.0 24 821.0 25 887.0 26 988.0 27 1151.0 28 1389.0 29 1600.0 30 1924.0 31 2312.0 32 2921.0 33 3477.0 34 4558.0 35 6170.0 36 8325.0 37 8189.0 38 2439.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.63236059544051 18.909400048579062 14.353377979804991 12.104861376175439 2 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22.38635082271509 23.23378639766153 27.600204702963854 26.779658076659523 46-47 27.952120739005988 30.403330731199585 28.425709081446787 13.218839448347644 48-49 24.341437604628368 26.92017451795054 29.459879086469655 19.278508790951435 50-51 13.0930696504467 31.141469338190646 36.945094977881865 18.820366033480788 52-53 21.069476971116316 35.05768063145112 21.997571341833638 21.875271055598926 54-55 17.20805974550911 25.933957272593222 28.217783136292272 28.64019984560539 56-57 25.362400562143783 23.894580691055147 25.310350212105178 25.432668534695896 58-59 31.855450956574554 23.44713895275693 24.644483970326668 20.05292612034185 60-61 21.939687331910537 28.091544774695056 34.10459285478806 15.864175038606355 62-63 17.03737117873625 28.41528158506541 32.573128838613414 21.974218397584927 64-65 15.981608397675023 36.3659234839941 26.690379109915852 20.962089008415024 66-67 21.29486667938865 25.762017937997676 22.833648491577467 30.109466891036206 68-69 17.696397748267398 26.163814414211245 28.7321427022526 27.40764513526876 70-71 27.085610200364297 26.242518865469684 23.599618353716714 23.0722525804493 72-73 22.488507242605603 19.80657472460751 36.05082834591031 21.65408968687657 74-75 16.59452660797155 35.4642841653294 26.267077243353427 21.67411198334562 76-77 19.449721130743274 24.882250383824715 25.633419205981596 30.034609279450414 78-79 20.746877168632892 25.546495489243583 29.439625260235946 24.26700208188758 80-81 25.96576762585559 23.536709146272695 25.92846422777628 24.56905900009543 82-83 20.17298816670715 22.107610091265574 34.324357150293224 23.395044591734045 84-85 19.202936276551437 27.18225045120089 29.544113563792862 24.07069970845481 86-87 19.10321914659218 28.659166023299992 26.297070585275723 25.940544244832104 88-89 17.75236244045088 28.195694240765008 21.37346951171892 32.678473807065195 90-91 21.726792269441383 20.22546016262985 25.518306705660805 32.52944086226797 92-93 25.869817967272287 26.778246308154163 26.64983427907057 20.702101445502976 94-95 21.958456973293767 24.229961649921044 27.851527929616328 25.960053447168864 96-97 11.994064148847542 27.290162454873645 34.10684532074424 26.60892807553457 98-99 19.284176907588694 30.49451503159064 21.669096715961953 28.55221134485871 100-101 16.473845459516227 23.6532169173487 25.66243414714587 34.2105034759892 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 140.0 1 92.0 2 69.0 3 74.5 4 52.5 5 48.5 6 71.0 7 107.5 8 138.5 9 157.5 10 135.5 11 98.0 12 91.5 13 96.5 14 89.5 15 81.0 16 87.5 17 94.5 18 90.0 19 101.5 20 110.5 21 121.0 22 141.5 23 174.5 24 210.0 25 227.5 26 258.5 27 284.5 28 335.0 29 409.5 30 466.0 31 545.0 32 625.5 33 654.5 34 665.0 35 671.5 36 632.0 37 589.5 38 539.5 39 445.5 40 363.0 41 318.0 42 288.0 43 275.5 44 255.5 45 354.5 46 1191.0 47 6124.5 48 14978.5 49 14092.5 50 5657.0 51 1880.0 52 700.0 53 283.5 54 155.5 55 118.5 56 111.0 57 100.0 58 78.0 59 65.0 60 59.0 61 56.0 62 45.5 63 32.5 64 23.5 65 16.0 66 10.5 67 8.0 68 10.5 69 9.5 70 5.5 71 3.5 72 3.5 73 5.0 74 4.5 75 2.5 76 3.0 77 2.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012143290831815423 2 0.0156128024980484 3 0.010408534998698933 4 0.0 5 0.001734755833116489 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.673779165582445E-4 14-15 0.0 16-17 0.0 18-19 0.0026021337496747333 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0026021337496747333 28-29 0.0052042674993494666 30-31 8.673779165582445E-4 32-33 0.008673779165582443 34-35 0.0026021337496747333 36-37 0.0 38-39 0.0026021337496747333 40-41 0.0 42-43 0.0043368895827912216 44-45 8.673779165582445E-4 46-47 0.0 48-49 8.673779165582445E-4 50-51 0.0 52-53 0.0 54-55 8.673779165582445E-4 56-57 0.014745424581490155 58-59 0.030358227079538558 60-61 0.020817069997397866 62-63 0.012143290831815423 64-65 0.017347558331164886 66-67 0.003469511666232978 68-69 8.673779165582445E-4 70-71 0.0 72-73 0.0 74-75 0.0043368895827912216 76-77 0.0026021337496747333 78-79 0.008673779165582443 80-81 0.016480180414606645 82-83 0.01908231416428138 84-85 0.03642987249544627 86-87 0.00954115708214069 88-89 0.042501517911353974 90-91 0.05117529707693642 92-93 0.0312256049960968 94-95 0.0312256049960968 96-97 0.05030791916037818 98-99 0.057246942492844136 100-101 0.06158383207563535 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 57645.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.09888108248764 #Duplication Level Percentage of deduplicated Percentage of total 1 84.40359054030728 16.964177292046145 2 7.5004315553253935 3.0150056379564574 3 2.511651993785603 1.514441842310695 4 1.1651993785603314 0.936768149882904 5 0.7509062661833248 0.7546187874056727 6 0.6041774555498014 0.7285974499089253 7 0.4056620058691524 0.5707346690953248 8 0.21577766269635767 0.3469511666232978 9 0.1208354911099603 0.2185792349726776 >10 1.7089590885551527 6.9199410183016745 >50 0.20714655618850336 2.938676381299332 >100 0.224408769204212 10.413739266198283 >500 0.06904885206283445 10.320062451209992 >1k 0.10357327809425168 34.92583918813427 >5k 0.008631106507854307 9.431867464654351 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5437 9.431867464654351 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3240 5.620608899297424 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2507 4.349032873623038 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1925 3.3394049787492412 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1644 2.851938589643508 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1636 2.838060542978576 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1526 2.647237401335762 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1526 2.647237401335762 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1398 2.4251886546968517 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1331 2.3089600138780466 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1247 2.1632405238962615 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1143 1.9828259172521467 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1010 1.7521033914476538 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 995 1.7260820539509065 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 893 1.5491369589730246 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 876 1.5196461098100442 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 826 1.4329083181542197 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 1.0408534998698933 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 597 1.035649232370544 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 1.0321797207043109 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 567 0.9836065573770493 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 497 0.8621736490588949 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 486 0.8430913348946136 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 477 0.8274785323965652 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 366 0.6349206349206349 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 339 0.5880822274264897 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 313 0.542978575765461 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 309 0.5360395524329951 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 294 0.5100182149362478 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 267 0.4631798074421025 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 233 0.40419810911614185 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 218 0.37817677161939456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 202 0.3504206782895307 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 195 0.33827738745771535 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 193 0.33480787579148236 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 188 0.3261340966258999 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 166 0.28796946829733716 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 158 0.27409142163240524 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 156 0.27062190996617225 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 135 0.234192037470726 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.22204874663891058 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 123 0.21337496747332813 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 123 0.21337496747332813 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 112 0.19429265330904677 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.19255789747593027 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 107 0.1856188741434643 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 107 0.1856188741434643 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 99 0.1717408274785324 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 96 0.16653655997918293 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 91 0.1578627808136005 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91 0.1578627808136005 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 85 0.14745424581490155 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 83 0.14398473414866858 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 78 0.13531095498308612 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 75 0.13010668748373666 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 75 0.13010668748373666 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 74 0.12837193165062016 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 72 0.1249024199843872 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 70 0.12143290831815423 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 67 0.11622864081880475 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 67 0.11622864081880475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 64 0.11102437331945529 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 61 0.10582010582010583 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 60 0.10408534998698933 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 58 0.10061583832075637 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.673779165582445E-4 0.0 22-23 0.0 0.0 0.0 0.001734755833116489 0.0 24-25 0.0 0.0 0.0 0.001734755833116489 0.0 26-27 0.0 0.0 0.0 0.006939023332465956 0.0 28-29 0.0 0.0 0.0 0.028623471246422064 0.0 30-31 0.0 0.0 0.0 0.057246942492844136 0.0 32-33 0.0 0.0 0.0 0.08587041373926621 0.0 34-35 0.0 0.0 0.0 0.11102437331945528 0.0 36-37 0.0 0.0 0.0 0.1500563795645763 0.0 38-39 0.0 0.0 0.0 0.19255789747593027 0.0 40-41 0.0 0.0 0.0 0.2602133749674733 0.0 42-43 0.0 0.0 0.0 0.3339404978749241 0.0 44-45 0.0 0.0 0.0 0.4805273657732674 0.0 46-47 0.0 0.0 0.0 0.7858443924017695 0.0 48-49 0.0 0.0 0.0 0.9601873536299765 0.0 50-51 0.0 0.0 0.0 1.177899210686096 0.0 52-53 0.0 0.0 0.0 1.391274178159424 0.0 54-55 0.0 0.0 0.0 1.6263335935467083 0.0 56-57 0.0 0.0 0.0 1.8553213635180847 0.0 58-59 0.0 0.0 0.0 2.09992193598751 0.0 60-61 0.0 0.0 0.0 2.359267933038425 0.0 62-63 0.0 0.0 0.0 2.665452337583485 0.0 64-65 0.0 0.0 0.0 2.8944401075548614 0.0 66-67 0.0 0.0 0.0 3.2804232804232805 0.0 68-69 0.0 0.0 0.0 3.786971983693295 0.0 70-71 0.0 0.0 0.0 4.069737184491283 0.0 72-73 0.0 0.0 0.0 4.473935293607425 0.0 74-75 0.0 0.0 0.0 4.898083094804406 0.0 76-77 0.0 0.0 0.0 5.131407754358574 0.0 78-79 0.0 0.0 0.0 5.608465608465608 0.0 80-81 0.0 0.0 0.0 6.016133229247983 0.0 82-83 0.0 0.0 0.0 6.404718535866077 0.0 84-85 0.0 0.0 0.0 7.105559892445138 0.0 86-87 0.0 0.0 0.0 7.928701535258912 0.0 88-89 0.0 0.0 0.0 9.045016913869373 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGGGG 30 8.275941E-5 39.557655 50-51 GAGGGGG 35 2.0497247E-4 33.90656 50-51 CATGGGG 60 0.003932616 31.646124 4 GCAGAGA 145 0.0 31.154587 94-95 CACTGTC 40 4.4404107E-4 29.71984 86-87 ATCACTG 50 0.0016342549 23.77587 84-85 CTCTCTC 50 0.0016425529 23.755213 74-75 GGGGGTT 55 0.002858191 21.595648 52-53 AGTACTT 520 0.0 21.471058 12-13 GTACATG 475 0.0 21.00461 1 ACATGGG 430 0.0 20.974756 3 GTACTTT 535 0.0 20.869066 14-15 TACATGG 480 0.0 20.78581 2 TACTTTT 545 0.0 20.486147 14-15 GAGTACT 560 0.0 20.361609 12-13 ACTTTTT 565 0.0 20.181421 16-17 CCCCACG 60 0.0047200737 19.804615 82-83 TTTTTGG 90 1.2190798E-4 18.476276 42-43 CATGGGA 290 4.9996743E-8 18.005552 4 GTAAGCC 95 1.8320087E-4 17.519062 94-95 >>END_MODULE