FastQCFastQC Report
Thu 26 May 2016
SRR936599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819284
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96780.5319675212885949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74050.4070282594691098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.24460172243585937No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19730.10844925805976416No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.10388702368624139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132100.034.414351
GGTATCA83550.034.1142041
ATCAACG162550.027.6161613
TCAACGC162600.027.554
TATCAAC164300.027.2649422
GTATCGC700.00838598427.1474026
CAACGCA165700.026.8919775
AACGCAG168100.026.5377546
GTACATG122300.025.9465981
GGTAAGG5500.025.046893
TACATGG126100.024.5619562
GAGTACT83450.024.39577712-13
ACGCAGA186550.023.7094087
ACATGGG126050.023.6665673
CGCAGAG186850.023.621148
GTAAGGT6200.022.985824
TAAGGTG7100.022.7490355
AGTACTT87700.022.50928112-13
GTACTTT90450.022.40363714-15
GCAGAGT192900.022.3403059
Produced by FastQC (version 0.11.5)