FastQCFastQC Report
Thu 26 May 2016
SRR936599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819284
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104930.5767653648358365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88700.48755444449574664No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55780.3066041365724098No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23850.13109552988978082No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.12999619630579942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA145600.032.537421
GGTATCA95550.032.225051
ATCAACG183900.025.624063
TCAACGC184050.025.6017674
TATCAAC186050.025.3058932
CAACGCA188000.025.0884365
AACGCAG191550.024.6469126
GTACATG125100.024.3092751
TACATGG129900.023.2242812
GAGTACT107100.022.44043512-13
ACATGGG129000.022.3152073
ACGCAGA215500.021.885697
GTATAGA3500.021.7220751
AGTACTT110850.021.65986312-13
CGCAGAG218650.021.5921178
GTACTTT113950.021.0086614-15
CCCTATA3401.8189894E-1220.959942
ATGGGCG2951.3278623E-1020.9316545
TAAGGTG7500.020.8994485
GCAGAGT227600.020.4706279
Produced by FastQC (version 0.11.5)