FastQCFastQC Report
Thu 26 May 2016
SRR936598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855035
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102810.5542213489233356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78450.42290307190969445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46410.2501839587932303No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19510.10517321775599922No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.10479586638527036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA139200.035.366481
GGTATCA86200.034.6748621
ATCAACG171550.028.6410873
TCAACGC173650.028.3494514
TATCAAC174850.028.0725982
CAACGCA176800.027.7637235
AACGCAG179200.027.4441836
GTACATG140200.025.149381
TAAGGTG8650.024.720435
GAGTACT88450.024.60223612-13
ACGCAGA202100.024.239127
TACATGG144700.024.2017572
GGTAAGG7500.024.075873
CGCAGAG205100.023.8150778
ACATGGG143750.023.1723353
AGGTAAG6850.022.8913442
GCAGAGT210000.022.851519
GTACTTT95250.022.82060814-15
AGTACTT91050.022.5690412-13
GTGTAAG7400.021.833261
Produced by FastQC (version 0.11.5)