##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936598_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855035 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21054750988526 31.0 31.0 34.0 28.0 34.0 2 31.236583676318777 31.0 31.0 34.0 28.0 34.0 3 31.267474198600027 31.0 31.0 34.0 27.0 34.0 4 34.93738339168803 37.0 35.0 37.0 32.0 37.0 5 34.85695256423733 37.0 35.0 37.0 32.0 37.0 6 34.985940966073414 37.0 35.0 37.0 32.0 37.0 7 34.817070836938385 37.0 35.0 37.0 32.0 37.0 8 34.78947297490344 37.0 35.0 37.0 32.0 37.0 9 36.253498181975004 39.0 35.0 39.0 32.0 39.0 10-11 36.11802176239262 39.0 35.0 39.0 31.0 39.0 12-13 36.01252752643481 39.0 35.0 39.0 30.0 39.0 14-15 37.01621236256998 39.0 36.0 41.0 31.0 41.0 16-17 36.95867355602455 39.0 36.0 41.0 31.0 41.0 18-19 36.95284455549356 39.0 36.0 41.0 31.0 41.0 20-21 36.88086936365082 39.0 36.0 41.0 30.0 41.0 22-23 36.77934998531025 39.0 36.0 41.0 30.0 41.0 24-25 36.62590409345376 39.0 36.0 41.0 30.0 41.0 26-27 36.423549151363716 39.0 35.5 40.0 30.0 41.0 28-29 36.50464169139666 39.0 36.0 41.0 30.0 41.0 30-31 36.39573485136399 39.0 35.0 40.0 30.0 41.0 32-33 36.222194459942806 39.0 35.0 40.0 30.0 41.0 34-35 36.03991649753239 38.0 35.0 40.0 29.5 41.0 36-37 35.777147870525354 38.0 35.0 40.0 28.0 41.0 38-39 35.57396194680963 38.0 35.0 40.0 27.0 41.0 40-41 35.38335988269763 38.0 34.5 40.0 27.0 41.0 42-43 35.07902142008102 38.0 34.0 40.0 26.0 41.0 44-45 34.74688887271669 38.0 33.5 40.0 24.5 41.0 46-47 34.31164236793376 38.0 33.0 40.0 23.5 41.0 48-49 33.98142083572547 38.0 33.0 40.0 22.5 41.0 50-51 33.23238968536982 37.0 32.0 39.5 21.0 40.5 52-53 33.39054222696607 37.5 32.5 39.5 22.0 40.5 54-55 33.71984895163703 38.0 33.0 40.0 21.0 41.0 56-57 33.639692782076885 37.5 33.0 40.0 20.5 41.0 58-59 33.390284010813815 37.0 33.0 40.0 20.0 41.0 60-61 33.065028153107626 37.0 32.0 40.0 18.5 41.0 62-63 32.729804019870244 36.0 32.0 39.5 18.0 41.0 64-65 32.40607751336228 36.0 31.5 39.0 16.0 41.0 66-67 32.05420194228141 35.0 31.0 39.0 13.0 40.5 68-69 31.674386197565006 35.0 31.0 38.5 11.0 40.0 70-71 31.284713495971772 35.0 31.0 37.5 8.0 40.0 72-73 30.889038481753715 35.0 30.0 37.0 7.0 39.0 74-75 30.47284471721558 35.0 30.0 36.5 6.0 39.0 76-77 30.063197998959588 34.0 29.5 36.0 2.0 39.0 78-79 29.625434830070592 34.0 29.0 35.5 2.0 37.5 80-81 29.240984401911554 34.0 29.0 35.0 2.0 37.0 82-83 28.848234130353333 34.0 29.0 35.0 2.0 37.0 84-85 28.47597269054223 34.0 28.0 35.0 2.0 36.0 86-87 28.137908988240113 34.0 27.0 35.0 2.0 36.0 88-89 27.767752090930898 34.0 27.0 35.0 2.0 35.5 90-91 27.420700687588102 33.0 26.0 35.0 2.0 35.0 92-93 27.06633864051083 33.0 25.5 35.0 2.0 35.0 94-95 26.634863493141637 33.0 25.0 35.0 2.0 35.0 96-97 26.048912284673875 33.0 24.0 35.0 2.0 35.0 98-99 25.384111890072155 32.5 21.0 35.0 2.0 35.0 100-101 23.327482769866876 30.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27599692375586926 1101 2 0.3996010168424071 1101 3 0.3668210387577133 1101 4 0.3889943217191103 1101 5 0.4312502407450225 1101 6 0.31882792541100713 1101 7 0.31278183842700713 1101 8 0.30290486048055953 1101 9 0.5517222062935119 1101 10-11 0.43390737115987577 1101 12-13 0.503704239006062 1101 14-15 0.6449196075151846 1101 16-17 0.5905726830403282 1101 18-19 0.6801409814268524 1101 20-21 0.6234988676824216 1101 22-23 0.5471590021377395 1101 24-25 0.6591821641817432 1101 26-27 0.5399325006167999 1101 28-29 0.6194194901642405 1101 30-31 0.3760423450437429 1101 32-33 0.44255225900932516 1101 34-35 0.49229957852494977 1101 36-37 0.6362079671521883 1101 38-39 0.5753572151090296 1101 40-41 0.5776906246341156 1101 42-43 0.40269827022682847 1101 44-45 0.32822343095349993 1101 46-47 0.428153556577783 1101 48-49 0.4679463464170368 1101 50-51 0.5120275761992445 1101 52-53 0.6005608638225439 1101 54-55 0.616254997728511 1101 56-57 0.5463190555082917 1101 58-59 0.4374984070790475 1101 60-61 0.6179616227090534 1101 62-63 0.625672590603152 1101 64-65 0.6617009468125303 1101 66-67 0.491672720403578 1101 68-69 0.495229109493895 1101 70-71 0.544519312453513 1101 72-73 0.6534742686679849 1101 74-75 0.49348765882465884 1101 76-77 0.5647169151684786 1101 78-79 0.5625537111213177 1101 80-81 0.4886607396470666 1101 82-83 0.4817781285757583 1101 84-85 0.2761373005495571 1101 86-87 0.32077747122946576 1101 88-89 0.47539289623385983 1101 90-91 0.4583635680327447 1101 92-93 0.2227682066924821 1101 94-95 0.1384377766534577 1101 96-97 -0.024519684101623085 1101 98-99 0.1450579595337267 1101 100-101 0.41224572435936224 1102 1 0.19304366773389603 1102 2 0.19799410370232096 1102 3 0.31296858777267644 1102 4 0.36410518418643534 1102 5 0.2860109263697268 1102 6 0.3621006360180914 1102 7 0.06700535865407176 1102 8 0.25876136983789166 1102 9 0.3181524506492508 1102 10-11 0.46969153149719034 1102 12-13 0.5469599607104598 1102 14-15 0.500227888933594 1102 16-17 0.36032761614355735 1102 18-19 0.4287131234193069 1102 20-21 0.29637754637249003 1102 22-23 0.03174572139888454 1102 24-25 0.21646795387334805 1102 26-27 0.30333279393218504 1102 28-29 0.33452467912824346 1102 30-31 0.2871429730800159 1102 32-33 0.24477259006377494 1102 34-35 0.409957922999844 1102 36-37 0.19539572376363168 1102 38-39 0.11814119844568438 1102 40-41 0.04521179359705485 1102 42-43 0.32895734546158906 1102 44-45 0.3446153515054817 1102 46-47 0.32805217628870054 1102 48-49 0.5824116463857507 1102 50-51 0.5601188137183257 1102 52-53 0.31728039363292737 1102 54-55 0.3560063434076426 1102 56-57 0.50208903998913 1102 58-59 0.6259207464588528 1102 60-61 0.280909300049494 1102 62-63 0.5084171596855711 1102 64-65 0.4152773083743071 1102 66-67 0.6209420711588294 1102 68-69 0.6196566229822942 1102 70-71 0.6419079995827168 1102 72-73 0.41494651907334656 1102 74-75 0.14499074211294882 1102 76-77 0.20740749904940614 1102 78-79 0.3535550449477256 1102 80-81 0.35571625463997236 1102 82-83 0.29027250090296874 1102 84-85 0.18004688165852656 1102 86-87 0.2096336992821719 1102 88-89 -0.009889972946847791 1102 90-91 0.018594759342011713 1102 92-93 0.10326787007515392 1102 94-95 0.20345351706934167 1102 96-97 0.019114929641848022 1102 98-99 -0.3749518071098663 1102 100-101 0.10045945581775584 1103 1 0.17591751774683573 1103 2 0.3039898418576996 1103 3 0.3712748370118142 1103 4 0.23936541143707046 1103 5 0.41183687615443176 1103 6 0.20284334888781785 1103 7 0.35694224938983155 1103 8 0.07469106270067982 1103 9 0.3711978895241046 1103 10-11 0.47619989815495245 1103 12-13 0.3593412280312336 1103 14-15 0.49794658468228903 1103 16-17 0.5438545790115015 1103 18-19 0.5070882005284147 1103 20-21 0.40543117850553045 1103 22-23 0.30805258648787515 1103 24-25 0.2598477726170785 1103 26-27 0.4327534602980876 1103 28-29 0.4289326960201265 1103 30-31 0.2114448479438309 1103 32-33 0.2754117593416652 1103 34-35 0.337889168786333 1103 36-37 0.5453703020548204 1103 38-39 0.4668281953183495 1103 40-41 0.459042686548635 1103 42-43 0.3664381257290259 1103 44-45 0.5227917351983535 1103 46-47 0.4533227614790434 1103 48-49 0.5630058545327614 1103 50-51 0.19051197553441312 1103 52-53 0.35250007402527217 1103 54-55 0.6432621901449167 1103 56-57 0.7060720386459209 1103 58-59 0.622528840894617 1103 60-61 0.6583680959095872 1103 62-63 0.503264915874496 1103 64-65 0.39512536123509534 1103 66-67 0.37135753962926543 1103 68-69 0.305069114504132 1103 70-71 0.49256172624154715 1103 72-73 0.3432359449723883 1103 74-75 0.40109885752034913 1103 76-77 0.3792062051509255 1103 78-79 0.5116122177058848 1103 80-81 0.6589029962725448 1103 82-83 0.5214669580497464 1103 84-85 0.48459933682271483 1103 86-87 0.4837743483388053 1103 88-89 0.5505014190681798 1103 90-91 0.6101930891817808 1103 92-93 0.4316701847294162 1103 94-95 0.39441262136586985 1103 96-97 0.3891458039747171 1103 98-99 0.16029664533721544 1103 100-101 0.22241652330855644 1104 1 0.3038653479372364 1104 2 0.289378031904139 1104 3 0.3755343048023896 1104 4 0.26959788597393697 1104 5 0.39049168939101264 1104 6 0.22714269491710581 1104 7 0.3107286061309509 1104 8 0.2932198615130446 1104 9 0.5888279596198842 1104 10-11 0.5026416362304715 1104 12-13 0.48662551794947007 1104 14-15 0.5663738904404241 1104 16-17 0.271322437434641 1104 18-19 0.5062121299020035 1104 20-21 0.5275229683275597 1104 22-23 0.5409109184878815 1104 24-25 0.6137801791037205 1104 26-27 0.4757416801164993 1104 28-29 0.422762986671529 1104 30-31 0.5394684245094439 1104 32-33 0.3964997577943379 1104 34-35 0.4926501272007684 1104 36-37 0.6215147761137132 1104 38-39 0.6950937704135924 1104 40-41 0.5203019345937108 1104 42-43 0.6221380910963248 1104 44-45 0.2708580760610957 1104 46-47 0.6374841741692023 1104 48-49 0.32671134087917864 1104 50-51 0.1761580121154509 1104 52-53 0.5432605605438354 1104 54-55 0.6393952516149213 1104 56-57 0.515179147868551 1104 58-59 0.43747793993806283 1104 60-61 0.4390782890628415 1104 62-63 0.4009827525256142 1104 64-65 0.5142595740905094 1104 66-67 0.36767743540711706 1104 68-69 0.4637954918628573 1104 70-71 0.3905796876513996 1104 72-73 0.1495231979106002 1104 74-75 0.1887272364871606 1104 76-77 0.2773045458192307 1104 78-79 0.5205311508566695 1104 80-81 0.5545723643573979 1104 82-83 0.33204886843813597 1104 84-85 0.2939898620448389 1104 86-87 0.23191435964000462 1104 88-89 0.20304251755264247 1104 90-91 0.07740632636717137 1104 92-93 -0.05172548577790437 1104 94-95 0.1291084105560074 1104 96-97 0.3700392112046522 1104 98-99 0.29924592767069313 1104 100-101 0.47964154270084336 1105 1 0.11994844410110161 1105 2 0.19207274469525615 1105 3 0.20881786595202811 1105 4 0.20252669504917264 1105 5 0.2745518970312091 1105 6 0.16538476811228264 1105 7 0.2716146846173402 1105 8 -0.09466044473620627 1105 9 0.4282194696210837 1105 10-11 0.43753811676921117 1105 12-13 0.21908533275686182 1105 14-15 0.28099739226429676 1105 16-17 0.20894269237511054 1105 18-19 0.23611001341505755 1105 20-21 -0.013484467436256864 1105 22-23 -0.05877241016887069 1105 24-25 -0.00309409435600827 1105 26-27 -0.09237222135462275 1105 28-29 0.005192236681551776 1105 30-31 0.12481836638621502 1105 32-33 0.027991019268661432 1105 34-35 -0.019062202939132078 1105 36-37 0.06278977912008799 1105 38-39 -0.03711300795655603 1105 40-41 -0.23964465217022735 1105 42-43 0.0739278854603711 1105 44-45 0.1229905970054972 1105 46-47 0.025428616386705016 1105 48-49 -0.007511210930658763 1105 50-51 -0.04525788959617927 1105 52-53 0.06045751809383404 1105 54-55 0.06423641488104437 1105 56-57 0.096876846990682 1105 58-59 0.15826651171022377 1105 60-61 0.07038165219267967 1105 62-63 -0.04075573306383262 1105 64-65 -0.01789860124119258 1105 66-67 -0.2263564163586267 1105 68-69 0.07280459115334637 1105 70-71 0.11512622631336811 1105 72-73 0.11488476443162199 1105 74-75 0.11986925757186029 1105 76-77 0.1337802743637475 1105 78-79 0.07406163548407818 1105 80-81 0.21111573630593128 1105 82-83 0.2781736529813692 1105 84-85 -0.020554973005467048 1105 86-87 0.07259660600228912 1105 88-89 0.044424246818689284 1105 90-91 0.11977164083384295 1105 92-93 -0.031857866424875425 1105 94-95 0.04578075592819886 1105 96-97 0.08647355200715268 1105 98-99 0.07167547831806687 1105 100-101 0.33790706560941075 1106 1 -0.005967099260463016 1106 2 -0.08973965341202472 1106 3 -0.16618113997266093 1106 4 0.18772789589215222 1106 5 0.29877055383664697 1106 6 0.39170987050204076 1106 7 0.3339104021409014 1106 8 0.2815334397124829 1106 9 0.4436236606425936 1106 10-11 0.42501127673580896 1106 12-13 0.35102745769224697 1106 14-15 0.26321388198555695 1106 16-17 0.1931219837982283 1106 18-19 0.13014307635990718 1106 20-21 0.3436355969798228 1106 22-23 0.2613635793336897 1106 24-25 0.17578528454573217 1106 26-27 0.06448938348056288 1106 28-29 0.04915621139227255 1106 30-31 -0.05303214456849048 1106 32-33 0.048468970678257506 1106 34-35 -0.01769286419803251 1106 36-37 0.09721311603209415 1106 38-39 0.11206936510003374 1106 40-41 0.02528238270193839 1106 42-43 0.11750417079974795 1106 44-45 0.11216561390477864 1106 46-47 0.1131865789299411 1106 48-49 0.1016511812121692 1106 50-51 0.12454061133825434 1106 52-53 0.14356867559513375 1106 54-55 0.3418284662548601 1106 56-57 0.33183748160836757 1106 58-59 0.38613491602053074 1106 60-61 0.2578046113560717 1106 62-63 0.2583555636079993 1106 64-65 0.25009664707881996 1106 66-67 0.23052457294512152 1106 68-69 0.17700257119231466 1106 70-71 0.2759318249455198 1106 72-73 0.23434527976026587 1106 74-75 0.23126682805179755 1106 76-77 0.09360720585207716 1106 78-79 0.16218989108072535 1106 80-81 0.2577199750430559 1106 82-83 0.2175768101645552 1106 84-85 0.13033422736035405 1106 86-87 0.31000912737490793 1106 88-89 0.354597116515297 1106 90-91 0.38540248102629704 1106 92-93 0.17687210295760636 1106 94-95 0.2270324955599925 1106 96-97 0.4937974018620146 1106 98-99 0.43422816430955535 1106 100-101 0.5724347804290844 1107 1 0.40317993495204973 1107 2 0.29056020213665334 1107 3 0.5468329718104386 1107 4 0.4957564528075622 1107 5 0.513876885430669 1107 6 0.36930630933472486 1107 7 0.6378559981896927 1107 8 0.6858746895225636 1107 9 0.8004184286474185 1107 10-11 0.8693883048936115 1107 12-13 0.9194432722775261 1107 14-15 1.0160993651945134 1107 16-17 0.7862658750039273 1107 18-19 1.0428284026452843 1107 20-21 1.107571209560021 1107 22-23 0.9433654433680374 1107 24-25 1.2061924864759774 1107 26-27 1.151418834769622 1107 28-29 1.0055750544664335 1107 30-31 1.0590206686040204 1107 32-33 1.042898410628915 1107 34-35 0.9188099227827564 1107 36-37 1.1461416080918099 1107 38-39 1.1449401197164661 1107 40-41 1.094436752864688 1107 42-43 1.1810845793906353 1107 44-45 1.3504089599598075 1107 46-47 1.4301566047134813 1107 48-49 1.4126291416139125 1107 50-51 1.2444274143379417 1107 52-53 1.348496943154359 1107 54-55 1.4452737266867572 1107 56-57 1.4715915855452906 1107 58-59 1.5377545226231106 1107 60-61 1.5383131503114313 1107 62-63 1.5704222946768311 1107 64-65 1.4961932582553175 1107 66-67 1.4935682779741555 1107 68-69 1.3807123850383256 1107 70-71 1.6124212042583714 1107 72-73 1.6559304062628897 1107 74-75 1.4190361152514157 1107 76-77 1.4328715188919254 1107 78-79 1.5581771433081535 1107 80-81 1.6671550048290555 1107 82-83 1.6944433389561802 1107 84-85 1.685961734565911 1107 86-87 1.7384332983671804 1107 88-89 1.7961630697693032 1107 90-91 1.772571144386685 1107 92-93 1.6262597155196552 1107 94-95 1.7518752318354167 1107 96-97 1.6667326493454553 1107 98-99 1.685756156572225 1107 100-101 1.893053931227648 1108 1 0.11563215040276731 1108 2 -0.006972849519339519 1108 3 -0.05020941608652407 1108 4 -0.06584910804837563 1108 5 0.16184510911103445 1108 6 0.042362638313093726 1108 7 0.20313180184631108 1108 8 0.14272743198250026 1108 9 0.32463359379212164 1108 10-11 0.08093512888421373 1108 12-13 0.2784196282553779 1108 14-15 0.46917802067196845 1108 16-17 0.40073299209473845 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100-101 -4.886266185048992 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3951.0 3 1702.0 4 1732.0 5 1907.0 6 2290.0 7 2734.0 8 3402.0 9 4288.0 10 5408.0 11 7062.0 12 8844.0 13 10842.0 14 12355.0 15 15352.0 16 19102.0 17 23428.0 18 19988.0 19 13460.0 20 13381.0 21 14296.0 22 16085.0 23 17947.0 24 21110.0 25 23843.0 26 28101.0 27 32971.0 28 39192.0 29 46586.0 30 56063.0 31 68413.0 32 84374.0 33 105826.0 34 137268.0 35 183787.0 36 247306.0 37 304497.0 38 223943.0 39 32193.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.031040998919025 18.628696817185634 14.29018144764544 22.050080736249903 2 15.713553299574484 20.459361881179845 39.57624071321636 24.250844106029312 3 19.026480687088622 25.761510464990277 29.781837178196763 25.430171669724334 4 12.585780890557444 16.683958555548486 37.113372597784554 33.61688795610952 5 13.574613536053429 38.08190705881845 32.982373447349005 15.361105957779111 6 31.17741193425604 36.04790832565191 17.56509160261289 15.20958813747916 7 28.80747637750271 33.79737170735495 20.073676741141426 17.32147517400091 8 27.66779877788064 32.8833465344079 19.601735532422072 19.847119155289388 9 28.096996477766474 16.188687769875713 20.055650534912598 35.65866521744522 10-11 25.394228241305623 26.576218628448935 26.327147219619395 21.702405910626048 12-13 26.87289561722135 24.362695756302397 27.015266288431654 21.749142338044596 14-15 23.637059158762963 25.14472527134809 26.273385592765703 24.944829977123245 16-17 22.256292355894928 28.834628891053992 26.595795157200385 22.31328359585069 18-19 22.126429576498367 26.376490171480427 29.970318569187338 21.526761682833868 20-21 23.203344931352774 25.377555702575233 29.905851688776234 21.51324767729576 22-23 22.78356293187039 25.463777543130767 29.89892955854163 21.85372996645722 24-25 22.470023302106622 25.868545929678582 29.998477074581565 21.662953693633227 26-27 22.079990210187173 26.085808032875658 29.943215312000422 21.89098644493675 28-29 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27.552886279270606 22.572368570008486 54-55 22.368574975754626 26.7825538964298 27.788703245780443 23.060167882035135 56-57 22.577248061722976 27.205716663845458 27.37520471720185 22.841830557229713 58-59 22.86835976668212 26.48006986598238 27.567601807027568 23.083968560307927 60-61 22.659653982074044 26.770068993883893 28.037791516477323 22.532485507564743 62-63 22.644760196387345 26.81471638254832 27.977676640121402 22.562846780942934 64-65 22.32270001059316 26.845790809072167 27.901387111007963 22.930122069326707 66-67 22.36529258204548 27.16552762342705 27.4424559668031 23.02672382772437 68-69 22.499272205809415 26.782610570806288 27.84575615120868 22.87236107217562 70-71 22.400288538596755 27.007121005334888 27.622853313543928 22.969737142524426 72-73 22.60907559872169 26.94843316700378 27.564432934113658 22.878058300160866 74-75 22.343808036433874 27.505408422853762 27.343735259251194 22.807048281461174 76-77 22.51629799302743 27.108631184221093 27.53502323201884 22.840047590732638 78-79 22.567551936740053 27.155990462907063 27.418602939372377 22.857854660980504 80-81 22.386676731458767 27.65610762138409 27.164020165529884 22.79319548162726 82-83 22.57066844926993 27.590089300876297 26.95444388438596 22.884798365467812 84-85 22.32903247023153 27.39221919899682 26.99319477772105 23.285553553050597 86-87 22.59876360511486 27.206617683131622 26.988466819666083 23.20615189208743 88-89 22.467109971446387 27.21906442367279 26.794385668689113 23.519439936191713 90-91 22.330214074565685 27.14561135548929 27.224675588641706 23.29949898130332 92-93 22.469234379426883 26.932166696005126 27.373591450674052 23.22500747389394 94-95 22.167652736083184 26.65363288566887 27.520002846478576 23.65871153176937 96-97 22.310903236344874 26.56761886894779 27.483620967102844 23.637856927604485 98-99 22.051944144596867 26.842858421499304 27.071716111861775 24.03348132204205 100-101 21.86734551902437 26.67253948377452 27.447735163451988 24.012379833749122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 551.0 1 420.5 2 407.0 3 508.5 4 448.0 5 475.5 6 789.0 7 1520.5 8 2488.0 9 3019.5 10 2672.0 11 2231.0 12 2253.5 13 2402.5 14 2595.0 15 2754.5 16 2930.5 17 3102.5 18 3145.0 19 3328.0 20 3669.0 21 4091.5 22 4801.0 23 5608.0 24 6477.0 25 7818.5 26 9775.0 27 11965.0 28 13864.5 29 16537.5 30 20243.0 31 24447.5 32 30422.5 33 34577.0 34 37383.0 35 40086.0 36 43829.5 37 48720.5 38 50473.5 39 50233.5 40 50304.5 41 53138.0 42 56084.0 43 58273.5 44 60138.5 45 62228.5 46 62513.0 47 62991.5 48 69833.0 49 70950.0 50 65164.0 51 61109.5 52 58492.0 53 59768.0 54 61058.0 55 58121.5 56 53860.5 57 49764.0 58 46969.0 59 43369.5 60 37676.5 61 32209.5 62 27869.0 63 21965.5 64 16208.0 65 12927.5 66 9625.0 67 7496.0 68 5489.0 69 3488.5 70 2648.0 71 2191.0 72 1877.0 73 1728.5 74 1406.5 75 1062.0 76 768.5 77 479.5 78 302.0 79 217.0 80 150.5 81 92.5 82 72.0 83 49.0 84 30.5 85 18.5 86 12.0 87 11.0 88 10.0 89 9.0 90 6.5 91 2.5 92 0.5 93 2.5 94 4.5 95 2.5 96 2.5 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012398687895376638 2 0.006145436609012768 3 0.007331398059874881 4 0.03239831054400591 5 0.04242507553765832 6 0.05358389464349729 7 0.024096580387971116 8 0.02091604740611363 9 0.013261205314185447 10-11 0.04029573565997407 12-13 0.038840237515734205 14-15 0.01692690434412289 16-17 0.019622271277900415 18-19 0.027411881716517476 20-21 0.021994194179624643 22-23 0.012910807612794367 24-25 0.0028301352804664063 26-27 0.002452783909737552 28-29 0.0015094054829154165 30-31 0.00590285358497279 32-33 0.0012398687895376639 34-35 0.0014824518135776415 36-37 0.005660270560932813 38-39 0.005983714592986116 40-41 0.003450069675235238 42-43 7.816564107954836E-4 44-45 0.0012398687895376639 46-47 0.003045764635168609 48-49 5.660270560932813E-4 50-51 0.0017789421762931697 52-53 9.164247574843602E-4 54-55 0.002264108224373125 56-57 0.0019945715309953723 58-59 0.002964903627155283 60-61 8.355637494710343E-4 62-63 0.0014824518135776415 64-65 0.0034770233445730134 66-67 0.0022910618937109 68-69 0.006199343947688319 70-71 0.009191201244181378 72-73 0.0044743091100707 74-75 0.0031266256431819344 76-77 0.0028031816111286313 78-79 0.031886190826588175 80-81 0.020754325390086977 82-83 0.0037735137072885416 84-85 0.007331398059874881 86-87 0.006792324673119375 88-89 0.005579409552919487 90-91 0.011536170476567829 92-93 0.01323425164484767 94-95 0.006010668262323892 96-97 0.003881328384639643 98-99 0.020969954744789184 100-101 0.03929844989447638 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1855035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.98124129848928 #Duplication Level Percentage of deduplicated Percentage of total 1 72.04804851444132 31.68762606798906 2 13.975901311453956 12.293549758858587 3 5.403652391767644 7.129780192064746 4 2.67789507234813 4.7110859739591415 5 1.5169188069377628 3.3357986039073118 6 0.9305592626855551 2.4556290884830605 7 0.643166553035158 1.9801084354909824 8 0.4393971201525822 1.5460184613833605 9 0.31759738804011883 1.257149462324616 >10 1.738068464333779 14.324254558550988 >50 0.17989041834551425 5.491306947738093 >100 0.12038562592756792 9.605131170193214 >500 0.006420169095835557 1.9468662062709794 >1k 0.0018519718545679494 1.2514288508554652 >5k 1.2346479030452993E-4 0.4259940699019841 >10k+ 1.2346479030452993E-4 0.5582721520283362 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10281 0.5542213489233356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7845 0.42290307190969445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4641 0.2501839587932303 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1951 0.10517321775599922 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1944 0.10479586638527036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3907338675550594E-5 0.0 0.0 0.0 0.0 3 5.3907338675550594E-5 0.0 0.0 0.0 0.0 4 5.3907338675550594E-5 0.0 0.0 0.0 0.0 5 5.3907338675550594E-5 0.0 0.0 0.0 0.0 6 1.0781467735110119E-4 0.0 0.0 0.0 0.0 7 1.0781467735110119E-4 0.0 0.0 0.0 0.0 8 1.617220160266518E-4 0.0 0.0 0.0 0.0 9 1.617220160266518E-4 0.0 0.0 0.0 0.0 10-11 1.617220160266518E-4 0.0 0.0 0.0 0.0 12-13 1.617220160266518E-4 0.0 0.0 0.0 0.0 14-15 1.617220160266518E-4 0.0 0.0 0.0 0.0 16-17 1.617220160266518E-4 0.0 0.0 0.0 0.0 18-19 1.8867568536442707E-4 0.0 0.0 2.6953669337775297E-5 0.0 20-21 2.1562935470220238E-4 0.0 0.0 1.3476834668887648E-4 0.0 22-23 2.6953669337775296E-4 0.0 0.0 2.964903627155283E-4 0.0 24-25 3.234440320533036E-4 0.0 0.0 4.8516604807995536E-4 0.0 26-27 3.234440320533036E-4 0.0 0.0 8.355637494710342E-4 0.0 28-29 3.773513707288542E-4 0.0 0.0 0.0021023862083464732 0.0 30-31 3.773513707288542E-4 0.0 0.0 0.0042586797553684965 0.0 32-33 4.0430504006662947E-4 0.0 0.0 0.007007954027821577 0.0 34-35 5.121197174177307E-4 0.0 0.0 0.011077958097825647 0.0 36-37 5.929807254310566E-4 0.0 0.0 0.0156061745465719 0.0 38-39 7.007954027821577E-4 0.0 0.0 0.01964922494723819 0.0 40-41 7.007954027821577E-4 0.0 0.0 0.02326101663850008 0.0 42-43 7.816564107954836E-4 0.0 0.0 0.0273849280471797 0.0 44-45 9.164247574843602E-4 0.0 0.0 0.03107758074645492 0.0 46-47 0.0010242394348354612 0.0 0.0 0.037115202678116584 0.0 48-49 0.0010781467735110118 0.0 0.0 0.04498567412474697 0.0 50-51 0.0010781467735110118 0.0 0.0 0.052613562547337386 0.0 52-53 0.0010781467735110118 0.0 0.0 0.0653626481441051 0.0 54-55 0.0010781467735110118 0.0 0.0 0.09156161474042268 0.0 56-57 0.0011320541121865625 0.0 0.0 0.1402129878951071 0.0 58-59 0.0011320541121865625 0.0 0.0 0.1875705849215783 0.0 60-61 0.0011320541121865625 5.3907338675550594E-5 0.0 0.23473950626268508 0.0 62-63 0.0011320541121865625 5.3907338675550594E-5 0.0 0.28624796836717364 0.0 64-65 0.0011320541121865625 5.3907338675550594E-5 0.0 0.35301220731684313 0.0 66-67 0.0012398687895376636 5.3907338675550594E-5 0.0 0.4616354947480775 0.0 68-69 0.0012398687895376636 5.3907338675550594E-5 0.0 0.597616756557154 0.0 70-71 0.0012668224588754389 5.3907338675550594E-5 0.0 0.7086658742287881 0.0 72-73 0.0012937761282132143 5.3907338675550594E-5 0.0 0.8293913591926837 0.0 74-75 0.0013207297975509897 5.3907338675550594E-5 0.0 0.9586342036673163 0.0 76-77 0.001347683466888765 5.3907338675550594E-5 0.0 1.1006530874080542 0.0 78-79 0.001347683466888765 5.3907338675550594E-5 0.0 1.2116752514103508 0.0 80-81 0.0015094054829154168 5.3907338675550594E-5 0.0 1.337818423911139 0.0 82-83 0.0015094054829154168 5.3907338675550594E-5 0.0 1.47423094442962 0.0 84-85 0.0015094054829154168 5.3907338675550594E-5 0.0 1.6964639481195771 0.0 86-87 0.001536359152253192 5.3907338675550594E-5 0.0 2.005002601029091 0.0 88-89 0.0015633128215909672 5.3907338675550594E-5 0.0 2.3556698391135478 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13920 0.0 35.36648 1 GGTATCA 8620 0.0 34.674862 1 ATCAACG 17155 0.0 28.641087 3 TCAACGC 17365 0.0 28.349451 4 TATCAAC 17485 0.0 28.072598 2 CAACGCA 17680 0.0 27.763723 5 AACGCAG 17920 0.0 27.444183 6 GTACATG 14020 0.0 25.14938 1 TAAGGTG 865 0.0 24.72043 5 GAGTACT 8845 0.0 24.602236 12-13 ACGCAGA 20210 0.0 24.23912 7 TACATGG 14470 0.0 24.201757 2 GGTAAGG 750 0.0 24.07587 3 CGCAGAG 20510 0.0 23.815077 8 ACATGGG 14375 0.0 23.172335 3 AGGTAAG 685 0.0 22.891344 2 GCAGAGT 21000 0.0 22.85151 9 GTACTTT 9525 0.0 22.820608 14-15 AGTACTT 9105 0.0 22.56904 12-13 GTGTAAG 740 0.0 21.83326 1 >>END_MODULE