FastQCFastQC Report
Thu 26 May 2016
SRR936598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855035
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109120.5882368796276082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89470.4823089591301512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.30624759101580296No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.12732913395165052No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22720.12247747347085096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93350.035.3547971
GTATCAA141850.032.566511
ATCAACG180000.025.6762333
TCAACGC180950.025.5649284
CAACGCA184500.025.0703245
TATCAAC184350.024.9679782
GTACATG128050.024.8375171
AACGCAG188350.024.633516
GAGTACT107200.023.58841512-13
TACATGG131950.023.5795022
ACATGGG132300.022.9061363
GTACTTT115200.022.03277414-15
AGTACTT109800.021.86214412-13
ACGCAGA211450.021.8076527
GTAAGGT8550.021.6642024
CGCAGAG213700.021.5558228
GGTAAGG8300.021.1745723
GCTTATA8900.021.020781
TAAGGTG9300.020.936225
AGGTAAG7250.020.3107722
Produced by FastQC (version 0.11.5)