##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936598_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1855035 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.427867398728324 31.0 31.0 34.0 30.0 34.0 2 31.620257838800885 31.0 31.0 34.0 30.0 34.0 3 31.882529440145333 31.0 31.0 34.0 30.0 34.0 4 35.72343756317266 37.0 35.0 37.0 33.0 37.0 5 35.52536636775047 37.0 35.0 37.0 33.0 37.0 6 35.62779731918805 37.0 35.0 37.0 33.0 37.0 7 35.55380410612199 37.0 35.0 37.0 33.0 37.0 8 35.528454719183195 37.0 35.0 37.0 33.0 37.0 9 37.13791491804737 39.0 37.0 39.0 33.0 39.0 10-11 37.02322301196473 39.0 37.0 39.0 33.0 39.0 12-13 36.88875061656519 39.0 37.0 39.0 33.0 39.0 14-15 37.95674933357053 40.0 37.0 41.0 33.0 41.0 16-17 37.87902007239755 40.0 37.0 41.0 33.0 41.0 18-19 37.96906203926071 40.0 37.0 41.0 33.0 41.0 20-21 38.050611174452236 40.0 37.0 41.0 33.0 41.0 22-23 37.96156568474449 40.0 37.0 41.0 33.0 41.0 24-25 37.839951806839224 40.0 37.0 41.0 32.5 41.0 26-27 37.62150525461784 39.0 37.0 41.0 32.0 41.0 28-29 37.5242378176153 39.0 36.5 41.0 32.0 41.0 30-31 37.46338236205786 39.0 36.0 41.0 32.0 41.0 32-33 37.253787394846995 39.0 36.0 41.0 31.5 41.0 34-35 37.07516893212258 39.0 35.5 41.0 31.0 41.0 36-37 36.80607077494495 39.0 35.0 41.0 31.0 41.0 38-39 36.63122501731773 39.0 35.0 41.0 30.0 41.0 40-41 36.36291040330775 39.0 35.0 40.0 30.0 41.0 42-43 36.065576929815336 38.5 35.0 40.0 30.0 41.0 44-45 35.71629349311469 38.0 35.0 40.0 28.5 41.0 46-47 35.224146714212935 38.0 34.5 40.0 26.5 41.0 48-49 34.703836315756845 38.0 34.0 40.0 24.5 41.0 50-51 34.47109326778201 38.0 34.0 40.0 24.0 41.0 52-53 34.1702347934136 38.0 33.0 40.0 23.5 41.0 54-55 33.868121625737516 37.5 33.0 40.0 23.0 41.0 56-57 33.575137935402836 37.0 33.0 40.0 22.0 41.0 58-59 33.20777101240677 37.0 32.5 40.0 21.0 41.0 60-61 32.82694962628737 36.0 32.0 39.0 20.0 41.0 62-63 32.518572425857194 36.0 31.5 39.0 19.0 40.0 64-65 32.0767759098885 35.0 31.0 39.0 18.0 40.0 66-67 31.669584940445866 35.0 31.0 38.0 16.0 40.0 68-69 31.219740597886293 35.0 30.0 37.5 13.0 40.0 70-71 30.756978170223203 34.0 30.0 37.0 9.5 39.0 72-73 30.362347880228675 34.0 29.0 36.0 7.5 39.0 74-75 30.004400456056086 34.0 29.0 36.0 7.0 39.0 76-77 28.57831739023792 32.5 27.0 34.5 5.5 36.5 78-79 29.381232699113493 34.0 29.0 35.0 2.0 37.0 80-81 29.247306115518036 34.0 29.0 35.0 2.0 37.0 82-83 28.925934820636808 34.0 29.0 35.0 2.0 36.0 84-85 28.491198548814445 33.5 28.5 35.0 2.0 36.0 86-87 28.11940691145989 33.0 27.0 35.0 2.0 35.5 88-89 27.70920225224861 33.0 27.0 35.0 2.0 35.0 90-91 27.262676175921207 33.0 26.0 35.0 2.0 35.0 92-93 26.818630106709577 33.0 25.0 34.5 2.0 35.0 94-95 26.290517699126973 32.0 24.5 34.0 2.0 35.0 96-97 25.617193476133874 32.0 23.5 34.0 2.0 35.0 98-99 24.770040457457675 32.0 18.5 34.0 2.0 35.0 100-101 22.058038797111642 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33306557305851214 1101 2 0.295330764868897 1101 3 0.07940912484690799 1101 4 0.06081350734947932 1101 5 0.08237745669669749 1101 6 0.10521101613542072 1101 7 0.1148438168418906 1101 8 0.24428413661001258 1101 9 0.19985294466231096 1101 10-11 0.16900760147238714 1101 12-13 0.07766301558015698 1101 14-15 0.19478157040302335 1101 16-17 0.26335181461104895 1101 18-19 0.15349470617370997 1101 20-21 0.09024809066267636 1101 22-23 0.017323050626174563 1101 24-25 -0.03303805031544016 1101 26-27 0.031216663281377066 1101 28-29 0.07393914473739471 1101 30-31 -0.15411102962132617 1101 32-33 -0.008581570894023116 1101 34-35 0.04840552022537281 1101 36-37 0.09852879390759028 1101 38-39 0.13857743043077875 1101 40-41 0.14955415406330275 1101 42-43 0.20709289960134925 1101 44-45 0.1787099971581867 1101 46-47 0.20603815807123738 1101 48-49 0.08215721722621083 1101 50-51 -0.0038988533542010373 1101 52-53 0.045302054060066155 1101 54-55 0.12398011293547029 1101 56-57 0.08239743101587038 1101 58-59 0.12576871778362175 1101 60-61 0.21205974447353526 1101 62-63 0.045490495982129175 1101 64-65 0.13788695258138972 1101 66-67 -0.07516993208652067 1101 68-69 -0.14974438375226384 1101 70-71 0.024660801942143706 1101 72-73 0.05644717209555239 1101 74-75 -0.023212985839002442 1101 76-77 0.24515623232503359 1101 78-79 0.18059346611902427 1101 80-81 0.1337960408196217 1101 82-83 0.07555911395657944 1101 84-85 0.08828553414164375 1101 86-87 0.07276780181414111 1101 88-89 0.008749019485296827 1101 90-91 -0.09899690840892106 1101 92-93 0.01859467274049109 1101 94-95 -0.3076931554007416 1101 96-97 -0.4304775534414418 1101 98-99 -0.2769390454853742 1101 100-101 -0.11669886029464749 1102 1 0.4930638978736894 1102 2 0.519040635500378 1102 3 0.15489832622624888 1102 4 0.07322093758435244 1102 5 0.20098923419422476 1102 6 0.1344129644069767 1102 7 0.18953335452248865 1102 8 0.004190642332503103 1102 9 0.08832988558518196 1102 10-11 0.19130889452542732 1102 12-13 0.12169074463007234 1102 14-15 0.19561363154410571 1102 16-17 0.3882858344540878 1102 18-19 0.28724241069640044 1102 20-21 0.2740763146823042 1102 22-23 0.1332114675132985 1102 24-25 0.16015145417753018 1102 26-27 -0.09930433500235125 1102 28-29 0.04014782219477553 1102 30-31 0.2348487122105709 1102 32-33 0.23319066402378752 1102 34-35 0.4414011141732104 1102 36-37 0.39786970350134254 1102 38-39 0.30041134691807514 1102 40-41 0.4307943755669825 1102 42-43 0.21400698790571226 1102 44-45 0.2693268286567232 1102 46-47 0.2772303523421087 1102 48-49 0.447507125407121 1102 50-51 0.4809762821619259 1102 52-53 0.4696560016489073 1102 54-55 0.4242937359809602 1102 56-57 0.3509852660133319 1102 58-59 0.2884098046941048 1102 60-61 0.44727307768044966 1102 62-63 0.29739127762969275 1102 64-65 0.2745271165598595 1102 66-67 0.2546593412941611 1102 68-69 0.2072540959384881 1102 70-71 0.1005411431741301 1102 72-73 -0.001413869742982854 1102 74-75 -0.3497685371802568 1102 76-77 -0.195526026203396 1102 78-79 0.06551419510888934 1102 80-81 0.12958358313384366 1102 82-83 0.16017570207920073 1102 84-85 0.196177314041325 1102 86-87 -0.03101938017730177 1102 88-89 -0.06710524575651178 1102 90-91 0.08912950799145491 1102 92-93 0.221121752261201 1102 94-95 -0.04881823165325727 1102 96-97 0.023981914987643904 1102 98-99 -0.17702651857969443 1102 100-101 0.04184553052281004 1103 1 0.4487730919390245 1103 2 0.32939571078853547 1103 3 0.23675776310215113 1103 4 0.13667137445923316 1103 5 0.0019462055224224173 1103 6 -0.03589611651384672 1103 7 0.04965717921601964 1103 8 0.08523372010861152 1103 9 0.04233858727754125 1103 10-11 0.08490011753979587 1103 12-13 0.2071730307629167 1103 14-15 0.08338906322723005 1103 16-17 0.11791291793447556 1103 18-19 0.2646472685445431 1103 20-21 0.10376966340165694 1103 22-23 0.050183117960209245 1103 24-25 0.06457618699383971 1103 26-27 0.068290326336502 1103 28-29 0.13238310138856235 1103 30-31 0.12069986887274098 1103 32-33 0.13754404214433436 1103 34-35 0.12571297869442333 1103 36-37 0.08144116714726835 1103 38-39 0.24849019206662604 1103 40-41 0.25484482103511397 1103 42-43 0.3459753238119063 1103 44-45 0.2858901972530816 1103 46-47 0.2809960388663484 1103 48-49 0.4113290159024672 1103 50-51 0.1717094299219042 1103 52-53 0.3214603547457031 1103 54-55 0.41292391507869297 1103 56-57 0.2115370910055745 1103 58-59 0.1715851602655576 1103 60-61 0.06913769096868805 1103 62-63 0.0752100182883666 1103 64-65 0.1240985024573078 1103 66-67 -0.049808204339598205 1103 68-69 0.07024105976805117 1103 70-71 -0.06905362353437638 1103 72-73 0.1277588454489731 1103 74-75 0.19911237055377384 1103 76-77 0.06891741739979196 1103 78-79 0.1096575602474914 1103 80-81 0.16818340451692748 1103 82-83 0.2786467474178238 1103 84-85 0.2867383154008998 1103 86-87 0.1502082479516922 1103 88-89 0.147768039556901 1103 90-91 -0.07690033719698874 1103 92-93 0.1029966109584386 1103 94-95 0.30205646438597 1103 96-97 -0.09379195149569952 1103 98-99 -0.12103188015618827 1103 100-101 0.061041478189896026 1104 1 0.30636129899802 1104 2 0.39233406255551273 1104 3 0.19253296851862345 1104 4 0.07934969742638742 1104 5 -0.03856848502123 1104 6 0.1652368386276919 1104 7 0.1669101376790536 1104 8 0.16932460158173512 1104 9 0.21706385476958445 1104 10-11 0.13473791599368212 1104 12-13 0.10875742619950302 1104 14-15 0.20766830811282944 1104 16-17 0.04929928419452523 1104 18-19 0.2484295189815029 1104 20-21 0.03761247043269833 1104 22-23 0.15999245076425694 1104 24-25 0.24920734366529018 1104 26-27 0.2992508217664067 1104 28-29 0.12924096858127143 1104 30-31 0.2416610326530204 1104 32-33 0.2379341916725437 1104 34-35 0.23453839342608518 1104 36-37 0.3579039177533474 1104 38-39 0.41452715791796635 1104 40-41 0.421102924783348 1104 42-43 0.19505978507087463 1104 44-45 0.32095454116593203 1104 46-47 0.3929921938264229 1104 48-49 0.2369937460408167 1104 50-51 0.3481440541165881 1104 52-53 0.15440364825480657 1104 54-55 0.2871707550978897 1104 56-57 0.4458428825653229 1104 58-59 0.43867049949387393 1104 60-61 0.25415504782525034 1104 62-63 0.055784946440702754 1104 64-65 0.22246474023206275 1104 66-67 0.29249579178802065 1104 68-69 0.30336344079662325 1104 70-71 0.32433246728431087 1104 72-73 0.4966068575116829 1104 74-75 0.4318817157526915 1104 76-77 0.5146701520667243 1104 78-79 0.5657713712015493 1104 80-81 0.6346939877437627 1104 82-83 0.4853213144584281 1104 84-85 0.449580110792521 1104 86-87 0.6153531432738468 1104 88-89 0.4319783380259743 1104 90-91 0.4201203138167777 1104 92-93 0.6019281364377811 1104 94-95 0.6843809768446256 1104 96-97 0.5095459016413955 1104 98-99 0.5122819933797729 1104 100-101 0.6528338318566078 1105 1 0.3310055447991189 1105 2 0.336074792903851 1105 3 0.12864652926741016 1105 4 0.07032656839283646 1105 5 -0.010877780728122843 1105 6 0.09961371761113469 1105 7 0.14495358979754513 1105 8 0.005564430018026201 1105 9 -0.05612029562926324 1105 10-11 -0.018943388371575054 1105 12-13 0.019126408441849208 1105 14-15 0.17349686465269087 1105 16-17 0.18577110445347245 1105 18-19 -0.04722376598261491 1105 20-21 0.0453593939104735 1105 22-23 -0.03642144669836256 1105 24-25 0.01060600883544538 1105 26-27 -0.04730237100226731 1105 28-29 -0.1123998098690393 1105 30-31 -0.03565838451137182 1105 32-33 -0.030079497348182826 1105 34-35 0.02663163108993416 1105 36-37 -0.014858839291584047 1105 38-39 -0.04895642728265415 1105 40-41 0.009417652005062394 1105 42-43 0.02618176366549818 1105 44-45 -0.316488838799593 1105 46-47 0.004351776140929076 1105 48-49 0.1718050459967273 1105 50-51 -0.15373893782633274 1105 52-53 -0.17108839487662664 1105 54-55 -0.039545884177371704 1105 56-57 0.06450193976659335 1105 58-59 0.11634466873265836 1105 60-61 0.059785985412929676 1105 62-63 0.0014382314048262401 1105 64-65 0.21442770171039882 1105 66-67 0.023346194642069662 1105 68-69 0.024392021387477314 1105 70-71 -0.09258890602710679 1105 72-73 -0.23904522490303748 1105 74-75 -0.17123839889035608 1105 76-77 -0.056608959650116475 1105 78-79 0.01691329207380221 1105 80-81 -0.08357774833672593 1105 82-83 0.1018889378199539 1105 84-85 0.26504690038646217 1105 86-87 0.12291266946937895 1105 88-89 0.07923402007191171 1105 90-91 -0.03818679725870311 1105 92-93 -0.00273224356783075 1105 94-95 0.20339228080903382 1105 96-97 0.24423844984358567 1105 98-99 0.14769406697579512 1105 100-101 0.389915444876209 1106 1 0.2785740365880578 1106 2 0.2834633570614713 1106 3 0.0835423501748025 1106 4 0.06187494009747496 1106 5 -0.14632948854951167 1106 6 -0.08203565214511599 1106 7 -0.04513846157626489 1106 8 -0.05617659348191495 1106 9 -0.07247283449760289 1106 10-11 -0.17877727838708068 1106 12-13 -0.13061910827849488 1106 14-15 -0.03015139173921 1106 16-17 -0.04374597376037315 1106 18-19 0.008201400027957106 1106 20-21 -0.062145874688674496 1106 22-23 -0.08026513829643989 1106 24-25 -0.0657752588093885 1106 26-27 -0.14263717556705302 1106 28-29 -0.03295132285166602 1106 30-31 -0.20077876703155084 1106 32-33 -0.21953434644755276 1106 34-35 -0.37961762335358173 1106 36-37 -0.28368165530813627 1106 38-39 -0.13122162669416326 1106 40-41 0.01936337549306444 1106 42-43 -0.032123238521471364 1106 44-45 -0.050487705553742046 1106 46-47 -0.11034910659794406 1106 48-49 -0.16215853108387535 1106 50-51 -0.10926351136802737 1106 52-53 -0.07120221649975633 1106 54-55 -0.2611073776761259 1106 56-57 -0.35288543150065976 1106 58-59 -0.3390263201734598 1106 60-61 -0.06090072181200412 1106 62-63 -0.2878222759712159 1106 64-65 -0.02566136743748615 1106 66-67 -0.06282224147864923 1106 68-69 0.05059833177755024 1106 70-71 -0.07833386606528236 1106 72-73 0.14289342076875755 1106 74-75 0.12943508713294705 1106 76-77 0.11076200937449698 1106 78-79 0.021445281417310724 1106 80-81 0.1871020003610333 1106 82-83 0.15675096016676804 1106 84-85 0.3031065647866349 1106 86-87 0.1096522018563526 1106 88-89 -0.027526686119191623 1106 90-91 -0.04472184835417181 1106 92-93 0.36522423581504526 1106 94-95 0.05085192135139849 1106 96-97 0.13389857549470463 1106 98-99 0.13192554735275053 1106 100-101 0.024256770575046716 1107 1 0.6228211549315574 1107 2 0.6731585012746883 1107 3 0.3468034691850086 1107 4 0.22905296898320415 1107 5 0.08630035323434271 1107 6 0.2894672992425882 1107 7 0.299080027286017 1107 8 0.22097861118768947 1107 9 0.3592223255893785 1107 10-11 0.4451933358598552 1107 12-13 0.4695282343387248 1107 14-15 0.5611780706614979 1107 16-17 0.5002966563875546 1107 18-19 0.5094598216308057 1107 20-21 0.42333444296816936 1107 22-23 0.4848473727301652 1107 24-25 0.37457068170025565 1107 26-27 0.23367637904664917 1107 28-29 0.32610517416973295 1107 30-31 0.4798286144990911 1107 32-33 0.55450506359238 1107 34-35 0.5500469695181849 1107 36-37 0.48411737820593714 1107 38-39 0.7076339367226936 1107 40-41 0.6532056332876621 1107 42-43 0.6390871535833682 1107 44-45 0.7251278849845804 1107 46-47 0.7062140120539553 1107 48-49 0.7681669492678012 1107 50-51 0.6133350987698805 1107 52-53 0.4002361909981076 1107 54-55 0.6557781202727568 1107 56-57 0.7354813047838391 1107 58-59 0.6269388958572364 1107 60-61 0.5557210447573766 1107 62-63 0.5295090198501313 1107 64-65 0.5051714499982722 1107 66-67 0.6801536145144915 1107 68-69 0.7031961595652696 1107 70-71 0.6381849477557857 1107 72-73 0.8434772354175308 1107 74-75 0.9462487846106065 1107 76-77 0.9550438515937394 1107 78-79 0.9780208779876673 1107 80-81 1.0335034163014747 1107 82-83 1.0641076596921408 1107 84-85 1.205594976933245 1107 86-87 1.324840595181204 1107 88-89 1.422660567695619 1107 90-91 1.2739262618732994 1107 92-93 1.547135817772464 1107 94-95 1.435066504131985 1107 96-97 1.413231746688794 1107 98-99 1.4292377492943373 1107 100-101 1.618980385531767 1108 1 0.36696207148627025 1108 2 0.2713821167396837 1108 3 0.03751655420093769 1108 4 0.021689118440939126 1108 5 -0.15210966253699354 1108 6 -0.10764907763390852 1108 7 -0.003843186431801371 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62-63 -1.345368115896573 2316 64-65 -1.1101728138967175 2316 66-67 -1.3156010334140653 2316 68-69 -1.3371538554224287 2316 70-71 -0.9425516800822926 2316 72-73 -1.1642780259609218 2316 74-75 -1.4286719200202462 2316 76-77 -1.4866452966171444 2316 78-79 -1.2008276486869427 2316 80-81 -1.1142177567820148 2316 82-83 -0.943468097883617 2316 84-85 -1.2156342232814588 2316 86-87 -1.0865577220142164 2316 88-89 -1.170301489034557 2316 90-91 -1.3894221985766109 2316 92-93 -1.5131199490050413 2316 94-95 -1.5211101694359925 2316 96-97 -1.4866957546879789 2316 98-99 -2.212150250789314 2316 100-101 -3.2212420597631457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 33.0 5 92.0 6 179.0 7 463.0 8 1003.0 9 1903.0 10 3103.0 11 4953.0 12 7038.0 13 9083.0 14 11828.0 15 15883.0 16 20383.0 17 25778.0 18 21332.0 19 13836.0 20 12384.0 21 12566.0 22 13369.0 23 15090.0 24 17630.0 25 21408.0 26 26070.0 27 32248.0 28 39640.0 29 49299.0 30 59791.0 31 73078.0 32 90324.0 33 113141.0 34 146206.0 35 195107.0 36 267036.0 37 330677.0 38 192421.0 39 10647.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56443190498565 19.647670729589244 15.030018658554978 21.757878706870127 2 15.872432238926995 21.296283317141338 38.77118392776465 24.060100516167015 3 19.151362112920626 25.865670700380445 30.172473744561174 24.810493442137762 4 13.086963014799796 17.299602172282746 36.52429369271354 33.08914112020393 5 13.43343926125383 38.346176756772785 33.052853450204445 15.167530531768941 6 31.305285345020444 36.63855398954737 16.943022638386875 15.113138027045311 7 28.626791408248366 34.109868546954644 19.84151242429388 17.421827620503116 8 27.97844784599752 33.12525100604571 19.20147059219907 19.694830555757708 9 27.87824944082744 16.069344405551995 20.044550513673247 36.00785563994732 10-11 25.56432482231386 26.814684318525906 26.02040995101687 21.60058090814336 12-13 26.953972148144317 24.485439311191364 27.034752556429037 21.525835984235286 14-15 23.52560019773217 25.307372989558953 26.345089538765993 24.821937273942886 16-17 22.07405790187247 28.902204001541747 26.754993841086556 22.26874425549922 18-19 21.966890112585478 26.286539067996024 30.46670278458358 21.279868034834923 20-21 23.03846019077807 25.20971302428256 30.54775246828227 21.2040743166571 22-23 22.637793842993172 25.30788942476544 30.549159208704435 21.505157523536955 24-25 22.45146317994 25.686253898174428 30.615217502634724 21.24706541925085 26-27 22.047724166929463 26.008323293091507 30.44182454778481 21.502127992194218 28-29 21.717110458832316 26.04444121000412 30.702924742659842 21.53552358850372 30-31 21.857248477049886 25.697527458384194 30.615360522944453 21.829863541621464 32-33 21.644905540656993 25.94779432238525 30.455189798762934 21.952110338194828 34-35 22.491101207691315 25.824502823948116 30.05112594048496 21.633270027875607 36-37 22.69955484515269 25.44223701429536 29.916211695860724 21.941996444691227 38-39 22.189380611596082 25.787405766424992 30.064314271752256 21.95889935022667 40-41 22.217768683814075 25.80163017475631 30.160524153018308 21.820076988411305 42-43 22.324182763393946 25.972819208514114 29.497318447651434 22.205679580440503 44-45 22.271428929536686 26.119058009317047 29.482856074693803 22.12665698645246 46-47 22.78990208609502 26.54198362584953 28.517054205478498 22.151060082576954 48-49 23.004022187400345 26.654316970795 27.949094165442613 22.39256667636204 50-51 22.854222607002487 26.811712174539775 27.917006059750072 22.417059158707666 52-53 23.267881964894162 26.85808903064463 27.65453515799677 22.21949384646444 54-55 22.573878107323004 26.811531303442077 27.871261346163383 22.743329243071535 56-57 22.79225002742664 27.157384692890396 27.498391464776518 22.55197381490645 58-59 23.12137435155441 26.50682232415622 27.58778509908017 22.784018225209206 60-61 22.8508206456211 26.7847010490797 28.065166451337436 22.299311853961765 62-63 22.82731398271231 26.837126582646974 28.04658532088528 22.28897411375543 64-65 22.54030807919325 26.838254918844655 27.93058863302192 22.690848368940173 66-67 22.604512276939438 27.112746005779638 27.534479826131165 22.748261891149763 68-69 22.84959735163384 26.692499126025155 27.83649648887175 22.62140703346926 70-71 22.653740960635556 27.02481898360199 27.65357519346915 22.667864862293307 72-73 22.836448594352287 26.954027000575998 27.520835865855865 22.68868853921585 74-75 22.661782661782663 27.434981011139953 27.297571204855974 22.605665122221414 76-77 22.768845534941768 27.148567531798935 27.510235766287604 22.572351166971696 78-79 22.740885988799924 27.21482373656802 27.354245100169344 22.690045174462707 80-81 22.624000974829663 27.674082309483616 27.099617562677825 22.602299153008897 82-83 22.78020380419033 27.634497098158505 26.880333712445825 22.704965385205337 84-85 22.600302395849592 27.422866549838034 26.897811606145716 23.07901944816666 86-87 22.766149471671806 27.19659609890593 26.842247709068246 23.19500672035402 88-89 22.664377354685268 27.303200012726386 26.72819372367936 23.304228908908982 90-91 22.545451702241472 27.36142326540472 26.97013096819837 23.122994064155435 92-93 22.693207143157494 27.058784524668216 27.17506622720054 23.07294210497375 94-95 22.41191359971534 26.83686865011012 27.292564647910485 23.45865310226406 96-97 22.6758866611369 26.690989020705363 27.237136751779627 23.39598756637811 98-99 22.291522166981746 26.953781756122364 26.853852895119985 23.900843181775908 100-101 22.019146216341678 27.070934057372874 27.12387837041502 23.78604135587043 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 975.0 1 697.5 2 521.0 3 616.5 4 536.0 5 515.5 6 841.0 7 1658.0 8 2490.0 9 2899.5 10 2739.0 11 2393.0 12 2382.5 13 2475.5 14 2708.5 15 3031.5 16 3153.5 17 3324.5 18 3571.0 19 3795.0 20 4107.0 21 4555.0 22 5195.5 23 5988.5 24 6890.0 25 8265.0 26 10259.0 27 12502.5 28 14368.5 29 17048.5 30 20998.5 31 25015.5 32 30570.0 33 34724.0 34 37450.5 35 39893.5 36 43685.5 37 48720.5 38 50514.0 39 49997.0 40 50097.0 41 52984.5 42 55812.0 43 57930.5 44 59496.5 45 61797.0 46 62321.5 47 62354.5 48 69568.5 49 70617.0 50 64724.0 51 61017.0 52 57788.0 53 59132.5 54 60884.0 55 57692.0 56 53228.5 57 49324.0 58 46888.5 59 43588.0 60 37453.5 61 31830.5 62 27649.0 63 21803.0 64 15944.5 65 12537.5 66 9375.5 67 7317.5 68 5442.5 69 3507.5 70 2618.0 71 2148.0 72 1856.0 73 1712.5 74 1433.0 75 1154.5 76 827.0 77 505.5 78 346.0 79 250.5 80 165.5 81 121.0 82 88.5 83 57.0 84 30.5 85 13.0 86 6.0 87 3.0 88 4.0 89 2.0 90 0.5 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9888762206643001 2 0.011266633783190073 3 0.004636031126097352 4 0.011428355799216725 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003342254997884137 10-11 4.582123787421801E-4 12-13 3.234440320533036E-4 14-15 2.6953669337775297E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014285444749020907 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.6953669337775297E-5 32-33 0.004501262779408474 34-35 4.3125870940440475E-4 36-37 0.003369208667221912 38-39 1.8867568536442707E-4 40-41 0.004339540763381822 42-43 0.012533456242065514 44-45 0.0026145059257642036 46-47 0.0020484788696709224 48-49 0.010700606727096792 50-51 4.3125870940440475E-4 52-53 0.002398876571062002 54-55 0.012021336524647784 56-57 0.004662984795435126 58-59 0.001185961450862113 60-61 0.006657556326430498 62-63 0.003935235723315193 64-65 0.020592603374060325 66-67 0.003962189392652969 68-69 2.4258302403997768E-4 70-71 0.0012668224588754389 72-73 2.6953669337775297E-5 74-75 0.0 76-77 0.001078146773511012 78-79 0.012183058540674436 80-81 0.018975383213793807 82-83 0.014258491079683133 84-85 0.009784181969612433 86-87 0.011967429185972232 88-89 0.03344950364817915 90-91 0.02905605554612177 92-93 0.003072718304506384 94-95 0.009703320961599107 96-97 0.012156104871336658 98-99 0.011617031484581155 100-101 0.013800278700940952 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1855035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.5861716487391 #Duplication Level Percentage of deduplicated Percentage of total 1 69.82010882032519 29.035510299356858 2 14.852205914699088 12.352927690621888 3 5.930545379555931 7.398860343745485 4 2.974363390438832 4.9476954600205945 5 1.6431540530775186 3.416624324830152 6 1.0275562429622165 2.5639278179136116 7 0.673332314847725 1.9600919255330238 8 0.4785999221719051 1.5922510811611217 9 0.35448118297952586 1.326736378947119 >10 1.9036233418391513 14.774852797042376 >50 0.20043409710584903 5.767335650752584 >100 0.1315378160412101 10.006932334685379 >500 0.007837031653965027 2.3433626310192297 >1k 0.0018286407192585061 1.1255919065385707 >5k 2.6123438846550087E-4 0.7945738060441179 >10k+ 1.3061719423275043E-4 0.592725551787901 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10912 0.5882368796276082 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8947 0.4823089591301512 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5681 0.30624759101580296 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2362 0.12732913395165052 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2272 0.12247747347085096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.3907338675550594E-5 0.0 0.0 0.0 0.0 6 2.1562935470220238E-4 0.0 0.0 0.0 0.0 7 2.1562935470220238E-4 0.0 0.0 0.0 0.0 8 2.1562935470220238E-4 0.0 0.0 0.0 0.0 9 2.1562935470220238E-4 0.0 0.0 0.0 0.0 10-11 2.1562935470220238E-4 0.0 0.0 0.0 0.0 12-13 2.1562935470220238E-4 0.0 0.0 0.0 0.0 14-15 2.1562935470220238E-4 0.0 0.0 0.0 0.0 16-17 2.1562935470220238E-4 0.0 0.0 0.0 0.0 18-19 2.1562935470220238E-4 0.0 0.0 2.6953669337775297E-5 0.0 20-21 2.1562935470220238E-4 0.0 0.0 1.0781467735110119E-4 0.0 22-23 2.6953669337775296E-4 0.0 0.0 2.4258302403997768E-4 0.0 24-25 3.773513707288542E-4 0.0 0.0 4.3125870940440475E-4 0.0 26-27 3.773513707288542E-4 0.0 0.0 7.816564107954837E-4 0.0 28-29 4.0430504006662947E-4 0.0 0.0 0.002048478869670923 0.0 30-31 4.3125870940440475E-4 0.0 0.0 0.0042317260860307215 0.0 32-33 4.3125870940440475E-4 0.0 0.0 0.006954046689146027 0.0 34-35 4.8516604807995536E-4 0.0 0.0 0.010889282412461222 0.0 36-37 5.390733867555059E-4 0.0 0.0 0.015336637853194144 0.0 38-39 5.390733867555059E-4 0.0 0.0 0.01959531760856264 0.0 40-41 5.660270560932813E-4 0.0 0.0 0.02315320196114898 0.0 42-43 6.199343947688319E-4 0.0 0.0 0.027142345023139725 0.0 44-45 6.468880641066072E-4 0.0 0.0 0.030861951391752716 0.0 46-47 7.27749072119933E-4 0.0 0.0 0.036899573323414384 0.0 48-49 7.547027414577084E-4 0.0 0.0 0.044635276423355894 0.0 50-51 7.547027414577084E-4 5.3907338675550594E-5 0.0 0.052290118515284076 0.0 52-53 7.547027414577084E-4 5.3907338675550594E-5 0.0 0.06506615778138958 0.0 54-55 7.547027414577084E-4 5.3907338675550594E-5 0.0 0.09134598538572049 0.0 56-57 8.086100801332589E-4 5.3907338675550594E-5 0.0 0.13975477551636492 0.0 58-59 8.086100801332589E-4 5.3907338675550594E-5 0.0 0.18740886290555164 0.0 60-61 8.086100801332589E-4 5.3907338675550594E-5 0.0 0.23487427460937393 0.0 62-63 8.086100801332589E-4 5.3907338675550594E-5 0.0 0.2866253197379025 0.0 64-65 8.086100801332589E-4 5.3907338675550594E-5 0.0 0.35376691005830074 0.0 66-67 9.703320961599107E-4 5.3907338675550594E-5 0.0 0.46276754886026406 0.0 68-69 9.703320961599107E-4 5.3907338675550594E-5 0.0 0.598317551959936 0.0 70-71 9.703320961599107E-4 8.08610080133259E-5 0.0 0.7092049476155436 0.0 72-73 9.703320961599107E-4 1.0781467735110119E-4 0.0 0.8306042743128836 0.0 74-75 9.703320961599107E-4 1.0781467735110119E-4 0.0 0.9600088408035428 0.0 76-77 9.703320961599107E-4 1.0781467735110119E-4 0.0 1.1020277245442807 0.0 78-79 9.703320961599107E-4 1.0781467735110119E-4 0.0 1.2139932669733993 0.0 80-81 0.0010242394348354614 1.0781467735110119E-4 0.0 1.3397051807647835 0.0 82-83 0.0010242394348354614 1.0781467735110119E-4 0.0 1.475524720557833 0.0 84-85 0.0010511931041732366 1.0781467735110119E-4 0.0 1.6991054077146792 0.0 86-87 0.0010781467735110118 1.0781467735110119E-4 0.0 2.009207373445784 0.0 88-89 0.0010781467735110118 1.0781467735110119E-4 0.0 2.3614109706824937 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9335 0.0 35.354797 1 GTATCAA 14185 0.0 32.56651 1 ATCAACG 18000 0.0 25.676233 3 TCAACGC 18095 0.0 25.564928 4 CAACGCA 18450 0.0 25.070324 5 TATCAAC 18435 0.0 24.967978 2 GTACATG 12805 0.0 24.837517 1 AACGCAG 18835 0.0 24.63351 6 GAGTACT 10720 0.0 23.588415 12-13 TACATGG 13195 0.0 23.579502 2 ACATGGG 13230 0.0 22.906136 3 GTACTTT 11520 0.0 22.032774 14-15 AGTACTT 10980 0.0 21.862144 12-13 ACGCAGA 21145 0.0 21.807652 7 GTAAGGT 855 0.0 21.664202 4 CGCAGAG 21370 0.0 21.555822 8 GGTAAGG 830 0.0 21.174572 3 GCTTATA 890 0.0 21.02078 1 TAAGGTG 930 0.0 20.93622 5 AGGTAAG 725 0.0 20.310772 2 >>END_MODULE