FastQCFastQC Report
Thu 26 May 2016
SRR936597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861825
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.525935573966404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76670.4118002497549448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44310.23799229250869441No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20240.10871053939011453No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19440.10441368012568313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134400.035.434921
GGTATCA83050.032.6210751
ATCAACG164900.028.707153
TCAACGC165250.028.7054124
TATCAAC166100.028.4425222
CAACGCA168500.028.1242965
AACGCAG171100.027.7802666
GTACATG134000.027.2407761
TACATGG137600.026.1474192
CGTATAG1658.365714E-825.925171
ACATGGG136700.024.9288373
ACGCAGA190000.024.743677
GAGTACT84500.024.46849612-13
CGCAGAG192750.024.3627098
GTATAGG6800.023.0657771
GCAGAGT199500.023.0136559
CGCGGAA3650.022.77218882-83
AATACCG1050.002187113522.6361277
CATGGGG46850.022.5225774
GTACTTT92650.022.41811614-15
Produced by FastQC (version 0.11.5)