##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936597_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1861825 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2246414136667 31.0 31.0 34.0 28.0 34.0 2 31.23664415291448 31.0 31.0 34.0 28.0 34.0 3 31.277009923059364 31.0 31.0 34.0 28.0 34.0 4 34.93443100183959 37.0 35.0 37.0 32.0 37.0 5 34.90883031434211 37.0 35.0 37.0 32.0 37.0 6 35.043123279577834 37.0 35.0 37.0 32.0 37.0 7 34.90005881326118 37.0 35.0 37.0 32.0 37.0 8 34.83634928094746 37.0 35.0 37.0 32.0 37.0 9 36.27338229962537 39.0 35.0 39.0 32.0 39.0 10-11 36.1719240530125 39.0 35.0 39.0 31.5 39.0 12-13 36.05218132746096 39.0 35.0 39.0 30.0 39.0 14-15 37.040584641413666 39.0 36.0 41.0 31.0 41.0 16-17 36.92227814107126 39.0 36.0 41.0 31.0 41.0 18-19 36.89764236703235 39.0 36.0 41.0 31.0 41.0 20-21 36.83512306473487 39.0 36.0 41.0 30.5 41.0 22-23 36.708374578706376 39.0 36.0 41.0 30.0 41.0 24-25 36.819095511124836 39.0 36.0 41.0 30.0 41.0 26-27 36.7923773716649 39.0 36.0 41.0 30.0 41.0 28-29 36.69061968767204 39.0 36.0 41.0 30.0 41.0 30-31 36.46588642863857 39.0 35.5 40.5 30.0 41.0 32-33 36.25516576477381 39.0 35.0 40.0 30.0 41.0 34-35 36.02700710324547 38.0 35.0 40.0 29.0 41.0 36-37 35.81197185557182 38.0 35.0 40.0 28.5 41.0 38-39 35.59143233118043 38.0 35.0 40.0 27.0 41.0 40-41 35.366735326897 38.0 34.0 40.0 27.0 41.0 42-43 35.088389349160096 38.0 34.0 40.0 26.0 41.0 44-45 34.76041679534865 38.0 33.5 40.0 24.5 41.0 46-47 34.371061189961466 38.0 33.0 40.0 23.5 41.0 48-49 34.19444818927665 38.0 33.0 40.0 23.0 41.0 50-51 33.23482980409007 37.0 32.0 39.5 21.0 40.5 52-53 33.35071824688142 37.5 32.5 39.5 21.5 40.5 54-55 33.750276476038295 38.0 33.0 40.0 21.5 41.0 56-57 33.65619163992319 38.0 33.0 40.0 20.5 41.0 58-59 33.39058128449237 37.0 33.0 40.0 19.5 41.0 60-61 33.06336390369664 37.0 32.0 40.0 18.5 41.0 62-63 32.71564191049105 36.0 32.0 39.5 17.5 41.0 64-65 32.364345736038565 36.0 31.5 39.0 15.0 41.0 66-67 31.980178051105767 35.0 31.0 39.0 13.0 40.5 68-69 31.574907147556832 35.0 31.0 38.5 9.5 40.0 70-71 31.14704147811959 35.0 30.5 37.5 7.5 40.0 72-73 30.76881017281431 35.0 30.0 37.0 7.0 39.0 74-75 30.344020517502987 35.0 30.0 36.5 3.5 39.0 76-77 29.927862178239096 34.0 29.0 36.0 2.0 39.0 78-79 29.48978045734696 34.0 29.0 35.5 2.0 37.5 80-81 29.038598149664978 34.0 29.0 35.0 2.0 37.0 82-83 28.5746383252991 34.0 28.0 35.0 2.0 36.5 84-85 28.261099995971698 34.0 27.0 35.0 2.0 36.0 86-87 27.942455655069622 34.0 27.0 35.0 2.0 36.0 88-89 27.54482564150766 33.0 26.0 35.0 2.0 35.0 90-91 27.14001477045372 33.0 25.5 35.0 2.0 35.0 92-93 26.771819585621635 33.0 25.0 35.0 2.0 35.0 94-95 26.298940555637614 33.0 24.5 35.0 2.0 35.0 96-97 25.703582774965422 32.5 22.0 35.0 2.0 35.0 98-99 24.977987727095726 32.0 17.0 35.0 2.0 35.0 100-101 22.542872181864567 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5433182808512313 1101 2 0.5826041326430769 1101 3 0.5299371980934673 1101 4 0.5524763323458117 1101 5 0.5530620206727619 1101 6 0.40025735442427646 1101 7 0.4749654219692232 1101 8 0.5403689842295094 1101 9 0.6850623103017881 1101 10-11 0.7318818106669127 1101 12-13 0.840079491183154 1101 14-15 1.0154649166535776 1101 16-17 0.9367467836227092 1101 18-19 0.9359932535678865 1101 20-21 1.0380632459998083 1101 22-23 0.9722455911200925 1101 24-25 1.0431156952270797 1101 26-27 0.8723796819793677 1101 28-29 0.9152018122232803 1101 30-31 0.9299864820869104 1101 32-33 0.7662371842568731 1101 34-35 0.7758239784525571 1101 36-37 0.7030653693203348 1101 38-39 0.7137974586279299 1101 40-41 0.6371967997461425 1101 42-43 0.6038483955816076 1101 44-45 0.6684517649625548 1101 46-47 0.5813178547701199 1101 48-49 0.6346952292774546 1101 50-51 0.6659796475893174 1101 52-53 0.7775435724043405 1101 54-55 0.763379584946172 1101 56-57 0.5976657659864699 1101 58-59 0.5486744568037878 1101 60-61 0.5458046698682395 1101 62-63 0.4418152366103527 1101 64-65 0.5758871957631442 1101 66-67 0.607526140674338 1101 68-69 0.5625232076088658 1101 70-71 0.5153538709567975 1101 72-73 0.44775260298631636 1101 74-75 0.32748237014235926 1101 76-77 0.4485408178632362 1101 78-79 0.4073195120483284 1101 80-81 0.6949800417462804 1101 82-83 0.6717625972350696 1101 84-85 0.6772859035395911 1101 86-87 0.5628541809684116 1101 88-89 0.7047271587087636 1101 90-91 0.4851778426427664 1101 92-93 0.5293124386057713 1101 94-95 0.6472019973590228 1101 96-97 0.3556739539006948 1101 98-99 0.4600877738124787 1101 100-101 0.9036561990421497 1102 1 0.4364454767369672 1102 2 0.5035363338753207 1102 3 0.3196084729377411 1102 4 0.3731223258621199 1102 5 0.4754464767720208 1102 6 0.37631477418427295 1102 7 0.43189954115383955 1102 8 0.4192855108686828 1102 9 0.5059883588107041 1102 10-11 0.5446979333479689 1102 12-13 0.5373522435296749 1102 14-15 0.5004374203452144 1102 16-17 0.42930039718076074 1102 18-19 0.5135721993730016 1102 20-21 0.5938504126919355 1102 22-23 0.47916528322932805 1102 24-25 0.501803870965837 1102 26-27 0.44439412602777395 1102 28-29 0.4122665388726361 1102 30-31 0.45894406224203976 1102 32-33 0.42993091866289745 1102 34-35 0.19473957157509147 1102 36-37 0.31090722735790877 1102 38-39 0.24327589863676025 1102 40-41 0.3989845689808291 1102 42-43 0.6119498348945314 1102 44-45 0.24402630202317255 1102 46-47 0.47138619243157365 1102 48-49 0.661407065419823 1102 50-51 0.7451190513267747 1102 52-53 0.5732370904042341 1102 54-55 0.7779450965981525 1102 56-57 0.9698052800486252 1102 58-59 1.0657625850180565 1102 60-61 0.8125713713296179 1102 62-63 0.7842694518623219 1102 64-65 0.7298898605288286 1102 66-67 0.8649863177030426 1102 68-69 0.5965525412477071 1102 70-71 0.6473398639639107 1102 72-73 0.5802382395598009 1102 74-75 0.5017296162682641 1102 76-77 0.7824968492293927 1102 78-79 0.5907380287752524 1102 80-81 1.0369818196711549 1102 82-83 1.0678966714411544 1102 84-85 1.1301035325770492 1102 86-87 1.102369516100886 1102 88-89 0.9243136108363323 1102 90-91 1.037041056995161 1102 92-93 0.9514879142749351 1102 94-95 1.2128992918580614 1102 96-97 1.0331768393157716 1102 98-99 1.110804566079569 1102 100-101 1.1880021857692427 1103 1 0.3417664790351367 1103 2 0.4263700625925466 1103 3 0.31734891576769897 1103 4 0.46389999655675496 1103 5 0.5082355394650762 1103 6 0.3132911994196519 1103 7 0.2389383011310997 1103 8 0.3491820932691567 1103 9 0.5427096378776 1103 10-11 0.6244280220622258 1103 12-13 0.4464965771842557 1103 14-15 0.24228571648774988 1103 16-17 0.5632961529817848 1103 18-19 0.49110559077834637 1103 20-21 0.6333965243384654 1103 22-23 0.6106783061863084 1103 24-25 0.6450417252668643 1103 26-27 0.5181888229413119 1103 28-29 0.2501421535710264 1103 30-31 0.35136180385866567 1103 32-33 0.6095332798460902 1103 34-35 0.28557284312969244 1103 36-37 0.5005918778138394 1103 38-39 0.5496713875536514 1103 40-41 0.42871249543942724 1103 42-43 0.5887163977729273 1103 44-45 0.7006236529510801 1103 46-47 0.7718910791293823 1103 48-49 0.8323804820308922 1103 50-51 0.810398322090613 1103 52-53 0.753399321357854 1103 54-55 0.8318910587401476 1103 56-57 0.9130298519220048 1103 58-59 0.7141867639786881 1103 60-61 0.5944546033458309 1103 62-63 0.6223165025455941 1103 64-65 0.844995996392619 1103 66-67 0.6629829878293094 1103 68-69 0.7129815142380416 1103 70-71 0.8039265580603328 1103 72-73 0.7723029157156809 1103 74-75 0.6077048553519653 1103 76-77 0.5981012305771607 1103 78-79 0.6621040492363655 1103 80-81 0.6033314069892164 1103 82-83 0.605851208471325 1103 84-85 0.8139609040155058 1103 86-87 0.7753808586763178 1103 88-89 0.8845404623449689 1103 90-91 0.7248792955599193 1103 92-93 0.7536986662456755 1103 94-95 0.8297769328989126 1103 96-97 0.9237404466995649 1103 98-99 1.0023527720251515 1103 100-101 1.5063896990516703 1104 1 0.42940579007870383 1104 2 0.49627887718220975 1104 3 0.41258701100579387 1104 4 0.3478918911869471 1104 5 0.5082355394650762 1104 6 0.4151149076263394 1104 7 0.48311256658196555 1104 8 0.47278898283349235 1104 9 0.6617572569252204 1104 10-11 0.763232480015624 1104 12-13 0.44801633402315844 1104 14-15 0.7316474186154096 1104 16-17 0.5270752816545325 1104 18-19 0.7172960669688209 1104 20-21 0.860600171754875 1104 22-23 0.5982669586685745 1104 24-25 0.6105939035850056 1104 26-27 0.2811067560720062 1104 28-29 0.5252181414129709 1104 30-31 0.49143272584448283 1104 32-33 0.3504147388126526 1104 34-35 0.2078119515390071 1104 36-37 0.32835276940451763 1104 38-39 0.30245760842498015 1104 40-41 0.32992830091054515 1104 42-43 0.4162239965571217 1104 44-45 0.5286378373482421 1104 46-47 0.5968657498487318 1104 48-49 0.5656631568029269 1104 50-51 0.4535087577542356 1104 52-53 0.48921492419473367 1104 54-55 0.6401484042315317 1104 56-57 0.48876440106081276 1104 58-59 0.4312586991357321 1104 60-61 0.4908578454937569 1104 62-63 0.6015464924138811 1104 64-65 0.5762523287127834 1104 66-67 0.6366405359549532 1104 68-69 0.5298508151499028 1104 70-71 0.5909073078070399 1104 72-73 0.6730121355738348 1104 74-75 0.5307038421807384 1104 76-77 0.5601073096045148 1104 78-79 0.6481729448797289 1104 80-81 0.6620953380935752 1104 82-83 0.5554459399809524 1104 84-85 0.6148727581188496 1104 86-87 0.556789166680371 1104 88-89 0.5598190844320996 1104 90-91 0.5052744323380338 1104 92-93 0.3909833673601639 1104 94-95 0.41336355903872857 1104 96-97 0.3867596969528577 1104 98-99 0.6264662472024582 1104 100-101 1.083644005029381 1105 1 0.379666316209466 1105 2 0.32768197516947595 1105 3 0.3150226179803397 1105 4 0.3998180546266923 1105 5 0.5142546263362888 1105 6 0.3483318501962742 1105 7 0.4538090041950511 1105 8 0.38579864766754923 1105 9 0.4459178954001288 1105 10-11 0.45059340923982205 1105 12-13 0.2689866263051002 1105 14-15 0.07306171560963293 1105 16-17 0.31091686982951927 1105 18-19 0.4278492847611801 1105 20-21 0.3303906734390267 1105 22-23 0.300827353132604 1105 24-25 0.4108405953507983 1105 26-27 0.34541556135445717 1105 28-29 0.25869075417569576 1105 30-31 0.22239403464116947 1105 32-33 0.22592252576444594 1105 34-35 0.14058503102818776 1105 36-37 0.304026922814721 1105 38-39 0.2548721982929649 1105 40-41 -0.047936512811112664 1105 42-43 0.2919133753562022 1105 44-45 -0.05932608809084883 1105 46-47 0.20652370465916903 1105 48-49 0.26941616457597206 1105 50-51 0.3802201344581775 1105 52-53 0.0983168488154007 1105 54-55 0.2881486682731591 1105 56-57 0.49309415263405754 1105 58-59 0.46434192637187977 1105 60-61 0.20846191052083185 1105 62-63 0.36204202065140834 1105 64-65 0.4504908695843497 1105 66-67 0.614744175834538 1105 68-69 0.5933554821615452 1105 70-71 0.7196542835946254 1105 72-73 0.5862915325877367 1105 74-75 0.40286368751911183 1105 76-77 0.3015604793939026 1105 78-79 0.5876325497985917 1105 80-81 0.37365123120426347 1105 82-83 0.5420004294220675 1105 84-85 0.6917365657508654 1105 86-87 0.6935538777697623 1105 88-89 0.5580357917925411 1105 90-91 0.5681813384429084 1105 92-93 0.647214395691023 1105 94-95 0.6611083299269787 1105 96-97 0.8424662081081138 1105 98-99 0.7780591466071485 1105 100-101 1.1441424164800829 1106 1 0.2578864150751059 1106 2 0.2166763102028071 1106 3 0.3329685952457275 1106 4 0.330440448346387 1106 5 0.3767118439687991 1106 6 0.3450520512576176 1106 7 0.47105546284986133 1106 8 0.4606646656256359 1106 9 0.6124999789088363 1106 10-11 0.5190136235234561 1106 12-13 0.6139157994014184 1106 14-15 0.7451315664348428 1106 16-17 0.7676039352561546 1106 18-19 0.6318669546940683 1106 20-21 0.6129964498544211 1106 22-23 0.6439124931046294 1106 24-25 0.5072420695463151 1106 26-27 0.21226603422543633 1106 28-29 0.6039323136227921 1106 30-31 0.5561208579322994 1106 32-33 0.1908940153550489 1106 34-35 0.3192490174955651 1106 36-37 0.4556703920455689 1106 38-39 0.45915392748028694 1106 40-41 0.2662044337453011 1106 42-43 0.0017060688662695611 1106 44-45 0.30666454908194396 1106 46-47 0.35539100773140575 1106 48-49 0.3107748802453685 1106 50-51 0.2844238443811591 1106 52-53 0.5281633026761625 1106 54-55 0.4718300383192755 1106 56-57 0.4839866213651405 1106 58-59 0.5301259297679195 1106 60-61 0.5374842520730354 1106 62-63 0.758417514199941 1106 64-65 0.615511536828194 1106 66-67 0.6079214208085872 1106 68-69 0.48966434875324083 1106 70-71 0.6355806006239852 1106 72-73 0.8064294465222055 1106 74-75 0.44872249352796345 1106 76-77 0.43719530799766915 1106 78-79 0.6747018683813799 1106 80-81 0.7427814543001823 1106 82-83 0.678885318917974 1106 84-85 0.8077865485979707 1106 86-87 0.8761815882432877 1106 88-89 1.1098994006126617 1106 90-91 1.1783185332701827 1106 92-93 1.4095859390471297 1106 94-95 1.4211499917501946 1106 96-97 1.6915704557119255 1106 98-99 2.0364283042800366 1106 100-101 2.3520096295994897 1107 1 0.10642960215844255 1107 2 0.1644371854418587 1107 3 0.050146219157785055 1107 4 0.18028106344343797 1107 5 0.19426236447680623 1107 6 0.15602218150052494 1107 7 0.23692167121873098 1107 8 0.3081706396291466 1107 9 0.3851364641161794 1107 10-11 0.20004780987901682 1107 12-13 0.21176468928127434 1107 14-15 0.16481006684664834 1107 16-17 0.3144422326430103 1107 18-19 0.45656856708895077 1107 20-21 0.4393681003360115 1107 22-23 0.4866908384293822 1107 24-25 0.3582217436082331 1107 26-27 0.2712853338904111 1107 28-29 0.32536193811623093 1107 30-31 0.39586440426332103 1107 32-33 0.26027828274451537 1107 34-35 0.498579824912035 1107 36-37 0.2514660063690073 1107 38-39 0.3399413654806338 1107 40-41 0.2654965918205292 1107 42-43 0.40432460210840304 1107 44-45 0.4106529745966512 1107 46-47 0.2555901151342752 1107 48-49 0.2803964146851712 1107 50-51 0.05861198160169323 1107 52-53 0.2218212995056632 1107 54-55 0.24614645967889004 1107 56-57 0.28118724004632867 1107 58-59 0.4487806315834817 1107 60-61 0.4420807053356697 1107 62-63 0.45718135000249305 1107 64-65 0.41144663573331286 1107 66-67 0.29657360122219245 1107 68-69 0.3831007331045022 1107 70-71 0.620214895754085 1107 72-73 0.40952678566170775 1107 74-75 0.35984698149842487 1107 76-77 0.1973171219275791 1107 78-79 0.2828227362646025 1107 80-81 0.29224899046849373 1107 82-83 0.5271055164937799 1107 84-85 0.5706587223746595 1107 86-87 0.5393306213325211 1107 88-89 0.5288233037637369 1107 90-91 0.5480650698589038 1107 92-93 0.3911024737656348 1107 94-95 0.40967279402800116 1107 96-97 0.39296626528772904 1107 98-99 0.769383965634912 1107 100-101 1.1517557442180468 1108 1 0.011865359416805887 1108 2 0.23768962192718845 1108 3 0.12617196990182222 1108 4 0.2799042544408934 1108 5 0.32828017029882517 1108 6 0.21337302261486002 1108 7 0.1406473542648783 1108 8 0.26859445395810866 1108 9 0.4152216612703157 1108 10-11 0.16404058025545254 1108 12-13 0.25436122263845107 1108 14-15 0.3996238976530222 1108 16-17 0.5195158700633371 1108 18-19 0.3600326552750346 1108 20-21 0.26127219925157164 1108 22-23 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78-79 -3.4112473987271947 2316 80-81 -2.8848271175132183 2316 82-83 -2.7265345948863278 2316 84-85 -2.499744370891815 2316 86-87 -3.238713612917504 2316 88-89 -3.487122357096851 2316 90-91 -3.502692884795156 2316 92-93 -4.076094307726834 2316 94-95 -4.496229747790846 2316 96-97 -5.262409308367321 2316 98-99 -6.662030138407804 2316 100-101 -6.844657794011846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4108.0 3 1720.0 4 1726.0 5 2014.0 6 2191.0 7 2680.0 8 3396.0 9 4250.0 10 5450.0 11 7232.0 12 9126.0 13 10912.0 14 12350.0 15 15709.0 16 19454.0 17 24130.0 18 19510.0 19 13818.0 20 13986.0 21 14866.0 22 16326.0 23 18705.0 24 21299.0 25 24388.0 26 28595.0 27 33776.0 28 39863.0 29 47103.0 30 57233.0 31 68833.0 32 84502.0 33 107199.0 34 138843.0 35 186605.0 36 248519.0 37 303996.0 38 218087.0 39 29321.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02951061011877 18.67015546463428 14.291946840605096 22.008387084641857 2 15.769434917524089 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29.01293733985433 22.521681518481092 46-47 22.65841522470534 26.555014029313316 28.273631765974727 22.512938980006616 48-49 22.784658510764576 26.76419230626689 27.68808266834103 22.763066514627504 50-51 22.66586530004654 26.95105513719241 27.631813932553612 22.751265630207442 52-53 23.045207808963355 26.87519000858328 27.52876582241086 22.5508363600425 54-55 22.414448327103802 26.800071869931543 27.738087276846684 23.047392526117978 56-57 22.59407505375986 27.19397438328591 27.30409950153611 22.90785106141812 58-59 22.893454431770934 26.49044437497113 27.491827037224446 23.12427415603349 60-61 22.65530996459021 26.81652442588009 27.964549384625965 22.56361622490374 62-63 22.664426446168235 26.825790001350903 27.92461171018329 22.585171842297573 64-65 22.366238699254065 26.85943999003128 27.817264145268293 22.957057165446358 66-67 22.36124733710266 27.177774650399044 27.425855162019108 23.035122850479187 68-69 22.554137983735302 26.765569899595302 27.78833816407214 22.891953952597248 70-71 22.39492830496776 27.00895147190344 27.59677285069332 22.999347372435484 72-73 22.573205143595764 27.019910875206758 27.513634191951052 22.893249789246426 74-75 22.31143073990341 27.51746615940009 27.31558544868975 22.85551765200675 76-77 22.551458101520424 27.07636551735807 27.495179768645013 22.876996612476496 78-79 22.495370513359376 27.275283577038824 27.347770796432847 22.88157511316896 80-81 22.38787008900346 27.667699999946283 27.120271506483896 22.824158404566365 82-83 22.607924039910525 27.569103640756946 26.90707946895187 22.915892850380658 84-85 22.374290999545263 27.373508033659743 26.944206361335937 23.307994605459058 86-87 22.61418722757413 27.180978060905225 26.92645877926354 23.278375932257102 88-89 22.46857591599976 27.254178821717034 26.728613333233064 23.54863192905014 90-91 22.329152162014527 27.199116534417183 27.170004280897796 23.301727022670494 92-93 22.486547872330426 26.923755653009373 27.3050861707051 23.2846103039551 94-95 22.158203665316044 26.649631260632578 27.502762389390277 23.689402684661097 96-97 22.33905064144469 26.56762228487847 27.39613151079925 23.69719556287759 98-99 22.036204248433606 26.81500709934626 27.01385863302531 24.13493001919483 100-101 21.87968209883757 26.671975228145904 27.33890356175998 24.109439111256542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 525.0 1 396.0 2 384.5 3 504.5 4 454.5 5 466.5 6 768.5 7 1459.0 8 2389.0 9 2891.5 10 2581.5 11 2214.0 12 2234.5 13 2400.0 14 2641.0 15 2781.5 16 2859.0 17 2969.5 18 3117.0 19 3328.0 20 3631.5 21 4083.5 22 4708.0 23 5463.5 24 6467.0 25 7870.5 26 9808.5 27 12053.5 28 13894.0 29 16525.0 30 20462.0 31 24490.5 32 30141.5 33 34644.5 34 37365.0 35 39921.0 36 43980.5 37 48969.0 38 50341.0 39 49952.5 40 50432.5 41 53432.0 42 56422.0 43 58648.0 44 60399.5 45 62587.0 46 63063.5 47 63528.5 48 70484.0 49 71566.5 50 65709.0 51 61407.0 52 58366.5 53 59820.0 54 61396.5 55 58502.0 56 54299.0 57 50060.5 58 47107.0 59 43484.5 60 37808.5 61 32475.5 62 28091.0 63 22115.5 64 16232.5 65 12787.5 66 9643.5 67 7576.0 68 5538.0 69 3593.0 70 2739.0 71 2195.5 72 1903.0 73 1807.5 74 1428.5 75 1067.0 76 797.5 77 483.0 78 285.0 79 213.0 80 151.0 81 87.5 82 60.0 83 46.0 84 29.0 85 15.5 86 11.5 87 11.5 88 8.5 89 6.0 90 7.5 91 4.5 92 1.5 93 5.5 94 4.5 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03383776670739731 2 0.023256750768734978 3 0.020839767432492315 4 0.04962872450418272 5 0.0547849556215004 6 0.04844708820646409 7 0.050971493024317534 8 0.04307601412592483 9 0.042109220791427766 10-11 0.05094463765391484 12-13 0.04538557598055671 14-15 0.03977280356639319 16-17 0.04756086098317511 18-19 0.05250224913727122 20-21 0.01176265223638097 22-23 0.01968498650517637 24-25 0.005397929450941952 26-27 0.009990197789803016 28-29 0.005451640191747345 30-31 0.009157681307319431 32-33 0.0034106320411424274 34-35 0.007492648342352261 36-37 0.008620573899265505 38-39 0.010930135753897386 40-41 0.001718743705772562 42-43 0.001987297409799525 44-45 0.0023364172250345764 46-47 4.8339666724853304E-4 48-49 0.0012890577793294214 50-51 2.954090744296591E-4 52-53 0.0035986196339613014 54-55 0.006901830193492943 56-57 0.016435486686450125 58-59 0.010688437420273119 60-61 0.010366172975440765 62-63 0.00555906167335813 64-65 5.371074080539256E-5 66-67 0.007036107045506426 68-69 0.016569763538463605 70-71 0.006445288896647108 72-73 0.002873524633088502 74-75 0.00440428074604219 76-77 0.007546359083157654 78-79 0.006311012044633625 80-81 0.011386677050743223 82-83 0.005935036858995878 84-85 0.016838317242490568 86-87 0.004296859264431405 88-89 0.006230445933425537 90-91 0.003544908893155909 92-93 0.016999449464906745 94-95 0.011386677050743223 96-97 0.00902340445530595 98-99 0.02073234595088153 100-101 0.036550159118069636 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1861825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.899059639425325 #Duplication Level Percentage of deduplicated Percentage of total 1 71.80225921172453 31.520516593809724 2 14.101989986416125 12.381281988965204 3 5.546050091932556 7.3039915124696275 4 2.6260381186738724 4.611224159482745 5 1.546798658666039 3.395150328348177 6 0.9391317310476506 2.473619992232253 7 0.6319636006520318 1.9419825456478659 8 0.44197539460676766 1.5521843365600827 9 0.3163873375214471 1.2500195939111696 >10 1.7412237792024332 14.336474932029564 >50 0.17874236621850523 5.513335857736825 >100 0.11879603568747218 9.545728731248415 >500 0.006544506364045063 1.9741686853947389 >1k 0.0018522187822769044 1.2539188344147587 >5k 2.4696250430358726E-4 0.9464019077488879 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9792 0.525935573966404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7667 0.4118002497549448 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4431 0.23799229250869441 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2024 0.10871053939011453 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1944 0.10441368012568313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.371074080539256E-5 0.0 0.0 0.0 0.0 3 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 4 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 5 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 6 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 7 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 8 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 9 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 10-11 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 12-13 5.371074080539256E-5 0.0 0.0 0.0 5.371074080539256E-5 14-15 1.0742148161078512E-4 0.0 0.0 0.0 5.371074080539256E-5 16-17 1.342768520134814E-4 0.0 0.0 0.0 5.371074080539256E-5 18-19 1.6113222241617768E-4 0.0 0.0 0.0 5.371074080539256E-5 20-21 1.6113222241617768E-4 0.0 0.0 0.0 5.371074080539256E-5 22-23 2.1484296322157024E-4 0.0 0.0 8.056611120808884E-5 5.371074080539256E-5 24-25 2.1484296322157024E-4 0.0 0.0 4.296859264431405E-4 5.371074080539256E-5 26-27 2.1484296322157024E-4 0.0 0.0 9.130825936916735E-4 5.371074080539256E-5 28-29 3.2226444483235536E-4 0.0 0.0 0.0021484296322157024 5.371074080539256E-5 30-31 3.2226444483235536E-4 0.0 0.0 0.004672834450069153 5.371074080539256E-5 32-33 3.2226444483235536E-4 0.0 0.0 0.008056611120808884 5.371074080539256E-5 34-35 3.491198152350516E-4 0.0 0.0 0.011494098532354008 5.371074080539256E-5 36-37 4.296859264431405E-4 0.0 0.0 0.016220643723228553 5.371074080539256E-5 38-39 4.296859264431405E-4 0.0 0.0 0.02049064761725726 5.371074080539256E-5 40-41 4.5654129684583673E-4 0.0 0.0 0.024868072992896756 5.371074080539256E-5 42-43 5.371074080539256E-4 0.0 0.0 0.029567762813368606 5.371074080539256E-5 44-45 5.908181488593181E-4 0.0 0.0 0.03386462207780001 5.371074080539256E-5 46-47 6.445288896647107E-4 0.0 0.0 0.04060532004887678 5.371074080539256E-5 48-49 6.445288896647107E-4 0.0 0.0 0.04930646005935037 5.371074080539256E-5 50-51 6.445288896647107E-4 0.0 0.0 0.05749734803217273 5.371074080539256E-5 52-53 6.445288896647107E-4 0.0 0.0 0.07350314879217971 5.371074080539256E-5 54-55 6.445288896647107E-4 0.0 0.0 0.10481651068172357 5.371074080539256E-5 56-57 8.056611120808883E-4 0.0 0.0 0.1531830327769796 5.371074080539256E-5 58-59 8.056611120808883E-4 0.0 0.0 0.2043693687645187 5.371074080539256E-5 60-61 8.056611120808883E-4 0.0 0.0 0.25520658493682274 5.371074080539256E-5 62-63 8.056611120808883E-4 0.0 0.0 0.31222053630174695 5.371074080539256E-5 64-65 8.59371852886281E-4 0.0 0.0 0.382420474534395 5.371074080539256E-5 66-67 8.59371852886281E-4 0.0 0.0 0.4917755428141743 5.371074080539256E-5 68-69 9.130825936916735E-4 0.0 0.0 0.6321754192794704 5.371074080539256E-5 70-71 9.667933344970661E-4 0.0 0.0 0.7479489210854942 5.371074080539256E-5 72-73 0.0010205040753024587 0.0 0.0 0.8655485880789011 5.371074080539256E-5 74-75 0.0010742148161078512 0.0 0.0 0.9956360023095618 5.371074080539256E-5 76-77 0.0011279255569132437 0.0 0.0 1.141353242114592 5.371074080539256E-5 78-79 0.0011279255569132437 0.0 0.0 1.2610207726290064 5.371074080539256E-5 80-81 0.001235347038524029 0.0 0.0 1.3957541659393338 5.371074080539256E-5 82-83 0.001235347038524029 0.0 0.0 1.537120835739127 5.371074080539256E-5 84-85 0.0012622024089267252 0.0 0.0 1.7701717400937254 5.371074080539256E-5 86-87 0.0012890577793294214 0.0 0.0 2.0821774334322507 5.371074080539256E-5 88-89 0.001342768520134814 0.0 0.0 2.4351644220052906 5.371074080539256E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13440 0.0 35.43492 1 GGTATCA 8305 0.0 32.621075 1 ATCAACG 16490 0.0 28.70715 3 TCAACGC 16525 0.0 28.705412 4 TATCAAC 16610 0.0 28.442522 2 CAACGCA 16850 0.0 28.124296 5 AACGCAG 17110 0.0 27.780266 6 GTACATG 13400 0.0 27.240776 1 TACATGG 13760 0.0 26.147419 2 CGTATAG 165 8.365714E-8 25.92517 1 ACATGGG 13670 0.0 24.928837 3 ACGCAGA 19000 0.0 24.74367 7 GAGTACT 8450 0.0 24.468496 12-13 CGCAGAG 19275 0.0 24.362709 8 GTATAGG 680 0.0 23.065777 1 GCAGAGT 19950 0.0 23.013655 9 CGCGGAA 365 0.0 22.772188 82-83 AATACCG 105 0.0021871135 22.636127 7 CATGGGG 4685 0.0 22.522577 4 GTACTTT 9265 0.0 22.418116 14-15 >>END_MODULE