FastQCFastQC Report
Thu 26 May 2016
SRR936597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861825
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108510.5828152484793147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95460.5127227317282773No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56030.3009412807326145No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.13228955460368189No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22800.12246048903629503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97500.035.185751
GTATCAA143550.030.5184141
GTACATG128150.024.8422221
ATCAACG184050.023.8984343
TCAACGC184250.023.8705654
TACATGG132100.023.8052482
TATCAAC184950.023.757732
ACATGGG130650.023.5228043
CAACGCA187000.023.520165
AGGTAAG8150.023.3141692
AACGCAG190150.023.131156
TAAGGTG9550.022.8783845
GAGTACT108150.022.79568112-13
GTAAGGT8800.022.1289314
AGTACTT110450.021.54684812-13
GTACTTT113450.021.43736314-15
GGTAAGG9350.021.336933
CATGGGG44050.020.9177534
ACGCAGA214700.020.4192877
CGCAGAG217200.020.1623888
Produced by FastQC (version 0.11.5)