##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936597_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1861825 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781341962859024 31.0 31.0 34.0 30.0 34.0 2 31.9078893021632 31.0 31.0 34.0 30.0 34.0 3 32.00248949283633 31.0 31.0 34.0 30.0 34.0 4 35.79118445611161 37.0 35.0 37.0 35.0 37.0 5 35.556959972070416 37.0 35.0 37.0 33.0 37.0 6 35.66305479838331 37.0 35.0 37.0 33.0 37.0 7 35.59849717347227 37.0 35.0 37.0 33.0 37.0 8 35.569240932955566 37.0 35.0 37.0 33.0 37.0 9 37.17562069474843 39.0 37.0 39.0 33.0 39.0 10-11 37.04255877969197 39.0 37.0 39.0 33.0 39.0 12-13 36.94433660521263 39.0 37.0 39.0 33.0 39.0 14-15 37.9815935976797 40.0 37.0 41.0 33.0 41.0 16-17 37.913062183610165 40.0 37.0 41.0 33.0 41.0 18-19 38.07938071515851 40.0 37.0 41.0 33.0 41.0 20-21 38.131138801981926 40.0 37.0 41.0 33.0 41.0 22-23 38.03548319525197 40.0 37.0 41.0 33.0 41.0 24-25 37.90172975440764 40.0 37.0 41.0 33.0 41.0 26-27 37.66385884817316 39.5 37.0 41.0 32.0 41.0 28-29 37.56689377358237 39.0 36.5 41.0 32.0 41.0 30-31 37.482405166973265 39.0 36.5 41.0 32.0 41.0 32-33 37.302000187987595 39.0 36.0 41.0 32.0 41.0 34-35 37.11762276261195 39.0 35.5 41.0 31.0 41.0 36-37 36.8198334967035 39.0 35.0 41.0 30.5 41.0 38-39 36.62529131363044 39.0 35.0 41.0 30.0 41.0 40-41 36.36999583741759 39.0 35.0 40.0 30.0 41.0 42-43 36.06173270849838 38.5 35.0 40.0 30.0 41.0 44-45 35.733409423549475 38.0 35.0 40.0 28.5 41.0 46-47 35.26995260027124 38.0 34.5 40.0 27.0 41.0 48-49 34.832565896365125 38.0 34.0 40.0 25.5 41.0 50-51 34.479348220160325 38.0 34.0 40.0 24.0 41.0 52-53 34.16721308393646 38.0 33.0 40.0 23.5 41.0 54-55 33.86731781988103 37.5 33.0 40.0 23.0 41.0 56-57 33.53831589972205 37.0 33.0 40.0 22.0 41.0 58-59 33.192787721724656 36.5 32.5 39.5 21.0 41.0 60-61 32.81420675949673 36.0 32.0 39.0 20.0 40.5 62-63 32.408629167617796 36.0 31.0 39.0 18.5 40.0 64-65 31.980056396277845 35.0 31.0 38.5 17.5 40.0 66-67 31.51989929236099 35.0 30.5 38.0 14.0 40.0 68-69 31.235003289782874 35.0 30.0 37.5 12.5 40.0 70-71 30.81323916050112 34.5 30.0 37.0 9.0 39.0 72-73 30.380994722919716 34.0 29.5 36.5 7.5 39.0 74-75 29.864417171323836 34.0 29.0 36.0 6.5 39.0 76-77 28.446633813596875 32.5 27.0 34.5 2.0 36.5 78-79 29.23378378741289 34.0 29.0 35.0 2.0 37.0 80-81 29.052185355766518 34.0 29.0 35.0 2.0 37.0 82-83 28.72803351550226 34.0 29.0 35.0 2.0 36.0 84-85 28.325200811032186 33.0 27.5 35.0 2.0 36.0 86-87 27.942346353712082 33.0 27.0 35.0 2.0 35.5 88-89 27.538814603950428 33.0 26.5 35.0 2.0 35.0 90-91 27.065378862137955 33.0 25.5 35.0 2.0 35.0 92-93 26.58187531051522 32.0 25.0 34.0 2.0 35.0 94-95 25.991300202758048 32.0 24.5 34.0 2.0 35.0 96-97 25.220184496394666 32.0 21.5 34.0 2.0 35.0 98-99 24.274872504129014 31.0 10.0 34.0 2.0 35.0 100-101 21.176809044888753 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.331961453101421 1101 2 0.38252063140157233 1101 3 0.34125198298933057 1101 4 0.21020778451449473 1101 5 0.22269098253362785 1101 6 0.151152763085733 1101 7 0.1684903746472699 1101 8 0.3460665977159536 1101 9 0.3100988054760023 1101 10-11 0.37113132225727696 1101 12-13 0.3345667033090933 1101 14-15 0.4758223702609996 1101 16-17 0.4717104176245641 1101 18-19 0.5247849488999918 1101 20-21 0.366181450157832 1101 22-23 0.2933826793875127 1101 24-25 0.2690358888938178 1101 26-27 0.1897722297899378 1101 28-29 0.3230452838561959 1101 30-31 0.4122815427870634 1101 32-33 0.3076907027730371 1101 34-35 0.3284668267488158 1101 36-37 0.26547472765280133 1101 38-39 0.43509991014961 1101 40-41 0.48891865104516796 1101 42-43 0.4304698240303395 1101 44-45 0.417197616916134 1101 46-47 0.384521177626155 1101 48-49 0.43053752297199566 1101 50-51 0.30677150807246534 1101 52-53 0.44793702512410505 1101 54-55 0.5099200883901744 1101 56-57 0.5306915722125609 1101 58-59 0.5203007823911037 1101 60-61 0.4133706852313992 1101 62-63 0.5103674692687648 1101 64-65 0.4784373330593503 1101 66-67 0.40482995482478046 1101 68-69 0.39613654960792033 1101 70-71 0.4228183746851144 1101 72-73 0.4699979478003584 1101 74-75 0.5319464595428727 1101 76-77 0.21996079820135606 1101 78-79 0.08511358223983123 1101 80-81 0.21612567761321344 1101 82-83 0.490020378672142 1101 84-85 0.5317352164427334 1101 86-87 0.6557623172611855 1101 88-89 0.306934974058489 1101 90-91 0.1832377372283709 1101 92-93 0.22850569031888668 1101 94-95 0.39207343304506637 1101 96-97 0.5745107717849081 1101 98-99 0.8196793356547509 1101 100-101 0.9687372687710507 1102 1 0.17650229021124986 1102 2 0.23919612404376878 1102 3 0.2662863533747917 1102 4 0.1801729473707141 1102 5 0.11025420706984335 1102 6 0.10945215035935263 1102 7 0.18691884181293972 1102 8 0.11896851906293193 1102 9 0.09940409936401551 1102 10-11 0.21385975802284207 1102 12-13 0.1756142787117767 1102 14-15 0.32412326389101764 1102 16-17 0.30749582336484593 1102 18-19 0.24828262359490338 1102 20-21 0.13063769793012625 1102 22-23 0.15736081812506342 1102 24-25 0.17754064982869977 1102 26-27 -0.16190576637415433 1102 28-29 -0.2291483741711957 1102 30-31 0.023808139524852834 1102 32-33 0.2147612426851211 1102 34-35 0.1100403751304384 1102 36-37 0.19835859393927535 1102 38-39 0.2411819832354709 1102 40-41 0.2330528780085217 1102 42-43 0.31391231516193585 1102 44-45 0.4699524457431252 1102 46-47 0.566123580158532 1102 48-49 0.37855974066807363 1102 50-51 0.30207422205993595 1102 52-53 0.4636254087824696 1102 54-55 0.5108992200499713 1102 56-57 0.4769141183367651 1102 58-59 0.3119817700517018 1102 60-61 0.45924772411660797 1102 62-63 0.35384961364135137 1102 64-65 0.5566517066696868 1102 66-67 0.36617175770001253 1102 68-69 0.3291227844807061 1102 70-71 0.29040169104541746 1102 72-73 0.2963686260426357 1102 74-75 0.13551960057177936 1102 76-77 -0.02706626953800395 1102 78-79 -0.17574650483181387 1102 80-81 0.2887018228684042 1102 82-83 0.12412004430345291 1102 84-85 0.17685303048621392 1102 86-87 0.16913176071553693 1102 88-89 0.01789223172605503 1102 90-91 0.271574931925997 1102 92-93 0.44256317797100664 1102 94-95 0.6152008835262528 1102 96-97 0.8243866565040889 1102 98-99 0.8153331791973635 1102 100-101 1.291413660983821 1103 1 0.08594856742478285 1103 2 0.09589417430694525 1103 3 0.06234857691496032 1103 4 9.265861381066998E-4 1103 5 -0.19520301733523127 1103 6 0.030118990006471336 1103 7 -0.0201202270579941 1103 8 0.05454720045764816 1103 9 -0.019995658463351162 1103 10-11 -0.07392952089578131 1103 12-13 -0.06033105946500683 1103 14-15 -0.10105728445701345 1103 16-17 0.09613455986619357 1103 18-19 0.13483988012491466 1103 20-21 -0.015247375092343418 1103 22-23 0.025086372280298974 1103 24-25 -0.10181042635684179 1103 26-27 0.10811517661642966 1103 28-29 0.0659570512371559 1103 30-31 -0.08737659124343367 1103 32-33 -0.042840630446072225 1103 34-35 -0.04131211578808802 1103 36-37 0.16843529223665143 1103 38-39 0.2398738185580882 1103 40-41 0.0983358554963516 1103 42-43 0.21557678868356334 1103 44-45 0.15782234590869137 1103 46-47 0.22478912863012823 1103 48-49 0.11409733920515208 1103 50-51 0.05334068609236198 1103 52-53 0.11379252025601971 1103 54-55 0.025319920867922008 1103 56-57 0.1177719469378502 1103 58-59 0.2801464165537908 1103 60-61 0.14473991528472396 1103 62-63 0.2759296418124535 1103 64-65 0.32519227676383267 1103 66-67 0.4515468781685428 1103 68-69 0.45293045492823225 1103 70-71 0.3882383542524721 1103 72-73 0.2473170840454486 1103 74-75 0.17241926573104038 1103 76-77 0.024461055195729386 1103 78-79 0.15776367012556136 1103 80-81 0.36859948639671103 1103 82-83 0.2992195080640485 1103 84-85 0.21671035250168558 1103 86-87 -0.03702232780102932 1103 88-89 0.05305282554324009 1103 90-91 -0.07328785750737055 1103 92-93 0.0978687355858554 1103 94-95 0.24493622343284116 1103 96-97 0.2640890683458359 1103 98-99 0.4653705553310026 1103 100-101 0.627947132701518 1104 1 0.22829912466895763 1104 2 0.28079792304047757 1104 3 0.15505374408821027 1104 4 0.09819102382807898 1104 5 0.15130154193528966 1104 6 0.07469852394770982 1104 7 0.09132860779509855 1104 8 0.08798185091357169 1104 9 0.2034085968558017 1104 10-11 0.16492559561890374 1104 12-13 0.19042881895444452 1104 14-15 0.18972285738695405 1104 16-17 0.27495928124410796 1104 18-19 0.16928770180677333 1104 20-21 0.1377414800241752 1104 22-23 0.08688981706246324 1104 24-25 0.11298187354184108 1104 26-27 0.12685884429626526 1104 28-29 -0.06524862252170038 1104 30-31 -0.09598854666389656 1104 32-33 0.025801720111168436 1104 34-35 0.03112962686642362 1104 36-37 0.1180300237462717 1104 38-39 0.06434190366446302 1104 40-41 0.06971376836362708 1104 42-43 0.12895064886593133 1104 44-45 0.3855325789380757 1104 46-47 0.3972815298459338 1104 48-49 0.3311692743621961 1104 50-51 0.1022261977438248 1104 52-53 0.2786861372772975 1104 54-55 0.20667757031066714 1104 56-57 0.2829188567656189 1104 58-59 0.21125077319006635 1104 60-61 0.22832654142454345 1104 62-63 0.23362974312957618 1104 64-65 0.43588123319747396 1104 66-67 0.09085792173490503 1104 68-69 0.44254544986237576 1104 70-71 0.2914805021754745 1104 72-73 0.23591890775365698 1104 74-75 0.3299673913642742 1104 76-77 0.06042863371649787 1104 78-79 -0.04309752541648493 1104 80-81 0.23587405579893783 1104 82-83 0.27895608354530665 1104 84-85 0.30409637073876894 1104 86-87 0.3137882091797195 1104 88-89 0.2564469491501278 1104 90-91 0.28284182840955197 1104 92-93 0.393461440753029 1104 94-95 0.27837087388876824 1104 96-97 0.363379848487682 1104 98-99 0.49196630001185326 1104 100-101 0.8984638500267472 1105 1 0.06289297856895715 1105 2 0.20268990775843676 1105 3 0.1174212364160816 1105 4 0.06733128506742503 1105 5 -0.0026630532185905054 1105 6 0.07258012100501077 1105 7 0.15776846169206493 1105 8 0.09884296552332472 1105 9 0.011591712253625985 1105 10-11 0.04271137389172708 1105 12-13 -0.07172000441637749 1105 14-15 0.10243711932738364 1105 16-17 0.16912423948051014 1105 18-19 -0.01921272890480452 1105 20-21 0.172516571692654 1105 22-23 0.1928208394751536 1105 24-25 0.16428005291918168 1105 26-27 0.20535558752994376 1105 28-29 0.19224735236095825 1105 30-31 0.18013589382835704 1105 32-33 0.23296615077014593 1105 34-35 0.14478462562461658 1105 36-37 0.03989621129372267 1105 38-39 0.2463508052057719 1105 40-41 0.12770264624234784 1105 42-43 0.03591252115375454 1105 44-45 -0.008668672376437314 1105 46-47 7.385530244334859E-4 1105 48-49 -0.01490899931138756 1105 50-51 0.06705175575938682 1105 52-53 -0.010076893455227776 1105 54-55 0.104327928613138 1105 56-57 -0.07410374003826803 1105 58-59 0.11473559155522395 1105 60-61 0.02025250759180608 1105 62-63 0.09665283766710075 1105 64-65 0.20521785642878498 1105 66-67 0.33023125788063723 1105 68-69 0.3792452633640089 1105 70-71 0.16201386120481232 1105 72-73 0.23479673083666341 1105 74-75 -0.019007120525440513 1105 76-77 -0.17151435858330188 1105 78-79 -0.009186512503234212 1105 80-81 0.22435081381288313 1105 82-83 0.3281574840787478 1105 84-85 0.15515577454086582 1105 86-87 0.20227488140036698 1105 88-89 0.2927087924540679 1105 90-91 0.18856859793105585 1105 92-93 0.12724956302757207 1105 94-95 0.31822257050797376 1105 96-97 0.5304974598113255 1105 98-99 0.6940636099739628 1105 100-101 1.0524469078614729 1106 1 0.09014404827312816 1106 2 0.10317990585370396 1106 3 0.12179902698371237 1106 4 0.0568552593022531 1106 5 0.02482565692734795 1106 6 0.05719895939688513 1106 7 0.022364443300446624 1106 8 -5.291430665010921E-4 1106 9 -0.06127833218926071 1106 10-11 0.056010604189282276 1106 12-13 0.10535434276187061 1106 14-15 0.174062902129684 1106 16-17 0.17370139542960317 1106 18-19 0.11663705862350326 1106 20-21 0.08320964411292664 1106 22-23 -0.01959646641939372 1106 24-25 0.025123661022774968 1106 26-27 -0.0014051283915250679 1106 28-29 0.016662897344978944 1106 30-31 0.15049359939700224 1106 32-33 0.13501498768514608 1106 34-35 -0.04842079082479245 1106 36-37 -0.03842280991632663 1106 38-39 0.08927627245146397 1106 40-41 -0.05541954708098018 1106 42-43 -0.043638518832473494 1106 44-45 -0.04144926400835658 1106 46-47 -0.04086141150991551 1106 48-49 0.07056732930359289 1106 50-51 0.09931221896236053 1106 52-53 0.04748995709496029 1106 54-55 -0.0038108441260504833 1106 56-57 0.14765461365473698 1106 58-59 0.18275552860885824 1106 60-61 0.18790809700067257 1106 62-63 -0.019000605245452107 1106 64-65 0.009665676180834026 1106 66-67 -0.04597289226997603 1106 68-69 0.11566670244956967 1106 70-71 0.07507586345182915 1106 72-73 -0.09068523207338686 1106 74-75 -0.1513964302108839 1106 76-77 -0.27342344642288907 1106 78-79 0.08924044695432798 1106 80-81 0.19872095710078952 1106 82-83 0.41920380051014305 1106 84-85 0.283759848296004 1106 86-87 0.3987732417586365 1106 88-89 0.4587701031725544 1106 90-91 0.6789517635027771 1106 92-93 0.6189296989929574 1106 94-95 0.7657322559024422 1106 96-97 0.3572424997114645 1106 98-99 0.70494880515486 1106 100-101 1.027790423305909 1107 1 -0.10928165554445357 1107 2 0.08377292500854594 1107 3 -0.05241734577118251 1107 4 0.020935439884645746 1107 5 -0.13256582771476388 1107 6 0.03642396170209139 1107 7 0.03002919269444959 1107 8 -0.18311250691887437 1107 9 -0.016617739461835868 1107 10-11 0.026562035840278497 1107 12-13 0.047579855077934496 1107 14-15 0.04954458628032654 1107 16-17 0.0010562103435916015 1107 18-19 -0.05621650678995138 1107 20-21 -0.002281901636706607 1107 22-23 -0.049798329652084306 1107 24-25 -0.09290556119244542 1107 26-27 0.04069251654610895 1107 28-29 -0.1330725638293373 1107 30-31 -0.2455915593914071 1107 32-33 -0.11950426066618292 1107 34-35 -0.2173879328938142 1107 36-37 -0.14405314217354714 1107 38-39 0.11587484910963752 1107 40-41 -0.009382341815417305 1107 42-43 -0.21001979127568404 1107 44-45 -0.06091094468946068 1107 46-47 0.019864002085959953 1107 48-49 -0.13755890437306562 1107 50-51 0.015242738709787318 1107 52-53 -0.18891257535116068 1107 54-55 -0.06339185901118327 1107 56-57 -0.340379276279819 1107 58-59 -0.22516940967149424 1107 60-61 0.022757831341806423 1107 62-63 0.09473454960978245 1107 64-65 0.022207306663123205 1107 66-67 0.17540711165799294 1107 68-69 0.0353579661564396 1107 70-71 -0.023102006869969216 1107 72-73 0.11426398303606078 1107 74-75 0.05065239121602971 1107 76-77 -0.22148191481706192 1107 78-79 -0.13429954930524346 1107 80-81 0.15416194978299558 1107 82-83 0.11840601642654391 1107 84-85 0.14527692480336185 1107 86-87 0.18500172453626718 1107 88-89 -0.0029034148656279513 1107 90-91 0.08181475291653939 1107 92-93 0.1818016904368598 1107 94-95 0.3142730205644746 1107 96-97 0.44821913663010093 1107 98-99 0.5500651777534742 1107 100-101 0.711060400337189 1108 1 0.018673485024464753 1108 2 0.10298124290761379 1108 3 0.03750895294255585 1108 4 -0.05061407537292695 1108 5 -0.04145954202967772 1108 6 0.053061803544494524 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-0.880028192637873 2316 56-57 -0.8404574053566733 2316 58-59 -1.0929942140849533 2316 60-61 -0.8570811142879862 2316 62-63 -0.5682340562801826 2316 64-65 -0.8408375520331859 2316 66-67 -1.6096688860928303 2316 68-69 -1.4487579381092033 2316 70-71 -0.7771466890795793 2316 72-73 -0.5554178601182755 2316 74-75 -0.7163073352237284 2316 76-77 -0.7757191786048665 2316 78-79 -0.9626653587312752 2316 80-81 -1.450484985944776 2316 82-83 -1.0520514007346442 2316 84-85 -1.0232296107745356 2316 86-87 -1.291966615157822 2316 88-89 -0.9284598903149224 2316 90-91 -1.3255092104000283 2316 92-93 -1.2314706367425003 2316 94-95 -1.0227613052182427 2316 96-97 -1.054968617655856 2316 98-99 -1.3880779945860766 2316 100-101 -1.7760197181727477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 20.0 4 32.0 5 95.0 6 182.0 7 398.0 8 965.0 9 1849.0 10 3167.0 11 4772.0 12 6689.0 13 9236.0 14 11832.0 15 16344.0 16 20327.0 17 25964.0 18 22045.0 19 14185.0 20 12736.0 21 13196.0 22 13825.0 23 15674.0 24 18902.0 25 21444.0 26 26607.0 27 32773.0 28 40411.0 29 49569.0 30 60471.0 31 73539.0 32 91048.0 33 114785.0 34 148078.0 35 197872.0 36 269248.0 37 331828.0 38 182728.0 39 8986.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65857550665792 19.612802075257083 14.977430827002628 21.75119159108237 2 16.045506672646848 21.23574895055876 38.585221611372376 24.13352276542202 3 19.17808292757601 25.814491243430222 30.202847328715805 24.804578500277966 4 13.09552199685038 17.318236212893677 36.490526433388546 33.095715356867395 5 13.420474247668402 38.43868231593738 32.97991419112134 15.160929245272884 6 31.413277645917624 36.50984707982132 16.90409550649606 15.172779767764998 7 28.618264337410874 34.124206088112466 19.85718314019846 17.400346434278195 8 28.001503900742552 33.13904368025996 19.134719965625127 19.72473245337236 9 27.86991436731015 16.0562233421725 20.064196318702066 36.00966597181528 10-11 25.537484826674973 26.833421061112244 25.99491357918596 21.63418053302682 12-13 26.91559454745944 24.494161867128298 27.050516942912033 21.53972664250023 14-15 23.549899786574812 25.348665642734236 26.28946063074703 24.81197393994392 16-17 22.15845627502845 28.83317447797282 26.770659858042663 22.237709388956077 18-19 21.947068064936285 26.245216387147018 30.446175123870393 21.3615404240463 20-21 23.007559786768358 25.162810683066343 30.56484900567991 21.26478052448538 22-23 22.66981345401986 25.273459509193557 30.53639690341515 21.52033013337143 24-25 22.444219573543794 25.62717228244143 30.656017883681947 21.272590260332827 26-27 22.05403672060563 26.0054067874358 30.45743914007049 21.48311735188808 28-29 21.729348650771804 25.989190060226807 30.713684309473134 21.567776979528258 30-31 21.762670060514004 25.69703359731615 30.69214451279823 21.84815182937162 32-33 21.660247763141253 25.86355759167717 30.517743808002766 21.958450837178813 34-35 22.483006343027327 25.74562196287649 30.115937443682366 21.655434250413812 36-37 22.708565605593712 25.42001046954204 29.937840787219315 21.933583137644934 38-39 22.207325019189998 25.74564350814091 30.07766778556837 21.969363687100724 40-41 22.19633315151901 25.754549587909914 30.19280643703276 21.856310823538323 42-43 22.348616175985214 25.87023257675103 29.586873672498697 22.19427757476506 44-45 22.326706216312754 26.045617267207184 29.430093001646263 22.197583514833802 46-47 22.777950099969253 26.49540973965026 28.529545646805747 22.197094513574736 48-49 22.96189252089227 26.65128023061392 27.964892285100596 22.42193496339321 50-51 22.855355032763992 26.78007841873456 27.903587925663338 22.460978622838113 52-53 23.261103389932927 26.79893380600372 27.682156021511872 22.25780678255148 54-55 22.59491716149547 26.808092364462567 27.864461220729904 22.732529253312066 56-57 22.815248605312128 27.11657799335212 27.50947807668561 22.558695324650145 58-59 23.13842665090419 26.40369791581344 27.58029772712563 22.87757770615674 60-61 22.850628969244575 26.781152971969984 28.09100535978104 22.277212699004398 62-63 22.855928919092907 26.78742306466181 27.994834854739693 22.361813161505594 64-65 22.578936625089977 26.79233678194261 27.90488928997325 22.723837302994166 66-67 22.632149717040722 27.05542950940834 27.548513443251306 22.76390733029963 68-69 22.821535085893533 26.699977574892475 27.876960014824743 22.601527324389252 70-71 22.65757199863467 26.937488972504642 27.702411103952656 22.70252792490803 72-73 22.857805042916812 26.920675400331778 27.557693511035964 22.66382604571545 74-75 22.742536266195337 27.419937470641525 27.19857892216489 22.63894734099825 76-77 22.76285120301392 27.131429025683474 27.542142213167477 22.56357755813513 78-79 22.76122188854411 27.140187598597226 27.406336327126063 22.6922541857326 80-81 22.69356480449478 27.607179401788134 27.064319726703605 22.63493606701348 82-83 22.796419848644444 27.545873173519595 26.860830559448335 22.796876418387622 84-85 22.607332700216148 27.351978419584803 26.888984620409005 23.15170425979004 86-87 22.81041844256197 27.167317403048873 26.861151709398268 23.161112444990888 88-89 22.718837953031553 27.249482504808338 26.65609876174486 23.37558078041525 90-91 22.509825809277906 27.317213625301264 27.005591840999294 23.16736872442154 92-93 22.680486293749073 27.02473122062481 27.130892124891197 23.163890360734914 94-95 22.40144529092066 26.82019615199618 27.243690884563915 23.534667672519248 96-97 22.747884506360645 26.610384200876624 27.207521540316236 23.434209752446492 98-99 22.338101876756244 26.911407791434883 26.838841637932493 23.911648693876376 100-101 22.039359807575945 26.998775058460676 27.05251564210866 23.909349491854716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 911.0 1 657.5 2 494.0 3 576.0 4 513.5 5 515.5 6 845.5 7 1556.0 8 2434.5 9 2998.0 10 2776.0 11 2372.5 12 2359.5 13 2511.5 14 2774.0 15 2984.0 16 3177.5 17 3363.5 18 3594.5 19 3870.5 20 4160.0 21 4653.0 22 5321.0 23 5991.0 24 6825.0 25 8193.0 26 10177.5 27 12506.0 28 14390.5 29 16989.5 30 20760.5 31 24780.0 32 30442.0 33 34622.0 34 37228.5 35 39756.0 36 43534.0 37 48776.5 38 50462.0 39 49969.5 40 50360.0 41 52865.5 42 55570.5 43 58194.5 44 60257.0 45 61994.0 46 62039.5 47 62569.5 48 70090.5 49 70979.0 50 64945.0 51 61162.5 52 58270.5 53 59812.0 54 61193.0 55 58151.5 56 53989.5 57 49897.0 58 47373.0 59 43931.5 60 37771.0 61 31967.5 62 27592.0 63 21861.0 64 16223.5 65 12945.5 66 9560.5 67 7333.0 68 5445.5 69 3541.0 70 2667.0 71 2214.5 72 1941.5 73 1733.5 74 1408.0 75 1139.0 76 839.0 77 525.5 78 352.0 79 257.5 80 172.5 81 120.5 82 84.0 83 52.5 84 31.5 85 17.5 86 7.5 87 3.5 88 2.0 89 1.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0353416674499483 2 0.006445288896647108 3 0.004296859264431405 4 6.982396304701032E-4 5 6.982396304701032E-4 6 2.1484296322157024E-4 7 0.0 8 0.0 9 0.0019335866689941322 10-11 2.685537040269628E-4 12-13 7.519503712754958E-4 14-15 0.0026049709290615394 16-17 0.0036791857451693903 18-19 0.0 20-21 0.0 22-23 0.001718743705772562 24-25 8.59371852886281E-4 26-27 0.004887677413290723 28-29 0.006042458340606663 30-31 0.0013159131497321177 32-33 3.491198152350516E-4 34-35 0.009372524270541001 36-37 0.012595168718864555 38-39 0.009560511863359876 40-41 0.001906731298591436 42-43 5.102520376512293E-4 44-45 0.001611322224161777 46-47 1.342768520134814E-4 48-49 6.445288896647107E-4 50-51 0.001342768520134814 52-53 0.003356921300337035 54-55 0.0037597518563774793 56-57 0.001530756112953688 58-59 0.001047359445705155 60-61 0.004753400561277241 62-63 0.00620359056302284 64-65 0.011010701865105475 66-67 0.003920884078793657 68-69 0.003894028708390961 70-71 8.056611120808884E-5 72-73 0.0013696238905375102 74-75 0.007492648342352261 76-77 0.0034106320411424274 78-79 0.004511702227652975 80-81 0.005908181488593182 82-83 0.006230445933425537 84-85 0.007358371490338781 86-87 0.004726545190874546 88-89 0.01146724316195131 90-91 0.014689887610274866 92-93 0.001530756112953688 94-95 0.0029272353738938944 96-97 0.0013696238905375102 98-99 0.004055160930807138 100-101 0.00555906167335813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1861825.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.88458552848088 #Duplication Level Percentage of deduplicated Percentage of total 1 69.08434266074943 28.244847161922905 2 15.1614540548767 12.397395300854793 3 6.1098395865876824 7.49394777429427 4 3.0063895013027757 4.916599547917612 5 1.7164973946625497 3.508914227074781 6 1.01653644770039 2.4936402803294837 7 0.7165991340533858 2.0508501010088693 8 0.4974514841549346 1.6270478200161742 9 0.3793278889564333 1.3957797167439658 >10 1.9645832414500581 15.04700455357729 >50 0.20329292201675128 5.804831755296985 >100 0.1332722083124654 10.058392583910127 >500 0.00804078463001599 2.3699219444088873 >1k 0.0019772421221350795 1.1896226719927636 >5k 2.636322829513439E-4 0.8164172265116596 >10k+ 1.3181614147567196E-4 0.5847873341394164 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10851 0.5828152484793147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9546 0.5127227317282773 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5603 0.3009412807326145 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2463 0.13228955460368189 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2280 0.12246048903629503 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.371074080539256E-5 0.0 0.0 0.0 0.0 6 5.371074080539256E-5 0.0 0.0 0.0 0.0 7 5.371074080539256E-5 0.0 0.0 0.0 0.0 8 5.371074080539256E-5 0.0 0.0 0.0 0.0 9 5.371074080539256E-5 0.0 0.0 0.0 0.0 10-11 5.371074080539256E-5 0.0 0.0 0.0 0.0 12-13 5.371074080539256E-5 0.0 0.0 0.0 0.0 14-15 5.371074080539256E-5 0.0 0.0 0.0 0.0 16-17 1.0742148161078512E-4 0.0 0.0 0.0 0.0 18-19 1.6113222241617768E-4 0.0 0.0 0.0 0.0 20-21 1.6113222241617768E-4 0.0 0.0 0.0 0.0 22-23 1.6113222241617768E-4 0.0 0.0 8.056611120808884E-5 0.0 24-25 2.685537040269628E-4 0.0 0.0 4.296859264431405E-4 0.0 26-27 2.685537040269628E-4 0.0 0.0 8.59371852886281E-4 0.0 28-29 3.491198152350516E-4 0.0 0.0 0.002202140373021095 0.0 30-31 3.759751856377479E-4 0.0 0.0 0.004753400561277241 0.0 32-33 3.759751856377479E-4 0.0 0.0 0.008244598713627758 0.0 34-35 4.0283055604044423E-4 0.0 0.0 0.011816362977186363 0.0 36-37 4.8339666724853304E-4 0.0 0.0 0.016730895760879782 0.0 38-39 5.908181488593181E-4 0.0 0.0 0.021054610395713883 0.0 40-41 5.908181488593181E-4 0.0 0.0 0.025432035771353376 0.0 42-43 6.71384260067407E-4 0.0 0.0 0.03018543633263062 0.0 44-45 8.59371852886281E-4 0.0 0.0 0.03442858485625663 0.0 46-47 9.399379640943697E-4 0.0 0.0 0.04138412579055496 0.0 48-49 9.667933344970661E-4 0.0 0.0 0.050138976541833954 0.0 50-51 9.667933344970661E-4 0.0 0.0 0.05851785210747519 0.0 52-53 9.667933344970661E-4 0.0 0.0 0.07446994212667679 0.0 54-55 9.667933344970661E-4 0.0 0.0 0.10581015938662333 0.0 56-57 9.667933344970661E-4 0.0 0.0 0.1543915244451009 0.0 58-59 9.667933344970661E-4 0.0 0.0 0.20603440172948584 5.371074080539256E-5 60-61 9.667933344970661E-4 0.0 0.0 0.2573281591986357 5.371074080539256E-5 62-63 9.667933344970661E-4 0.0 0.0 0.3140467014891303 1.0742148161078512E-4 64-65 9.667933344970661E-4 0.0 0.0 0.3848374578706377 1.0742148161078512E-4 66-67 0.0010205040753024587 0.0 0.0 0.4946490674472628 1.0742148161078512E-4 68-69 0.0010205040753024587 0.0 0.0 0.635398063727794 1.0742148161078512E-4 70-71 0.0011010701865105475 0.0 0.0 0.7511715655338176 1.0742148161078512E-4 72-73 0.0011816362977186362 0.0 0.0 0.8698991580841379 1.0742148161078512E-4 74-75 0.0011816362977186362 0.0 0.0 0.9999328615739933 1.0742148161078512E-4 76-77 0.0011816362977186362 0.0 0.0 1.1476642541592255 1.0742148161078512E-4 78-79 0.0011816362977186362 0.0 0.0 1.2679763135633049 1.0742148161078512E-4 80-81 0.0012890577793294214 0.0 0.0 1.4026559961328267 1.0742148161078512E-4 82-83 0.0012890577793294214 0.0 0.0 1.5443986411182578 1.0742148161078512E-4 84-85 0.0012890577793294214 0.0 0.0 1.7766170289903722 1.0742148161078512E-4 86-87 0.0012890577793294214 0.0 0.0 2.0887032884401058 1.0742148161078512E-4 88-89 0.0012890577793294214 0.0 0.0 2.4437312851637505 1.0742148161078512E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9750 0.0 35.18575 1 GTATCAA 14355 0.0 30.518414 1 GTACATG 12815 0.0 24.842222 1 ATCAACG 18405 0.0 23.898434 3 TCAACGC 18425 0.0 23.870565 4 TACATGG 13210 0.0 23.805248 2 TATCAAC 18495 0.0 23.75773 2 ACATGGG 13065 0.0 23.522804 3 CAACGCA 18700 0.0 23.52016 5 AGGTAAG 815 0.0 23.314169 2 AACGCAG 19015 0.0 23.13115 6 TAAGGTG 955 0.0 22.878384 5 GAGTACT 10815 0.0 22.795681 12-13 GTAAGGT 880 0.0 22.128931 4 AGTACTT 11045 0.0 21.546848 12-13 GTACTTT 11345 0.0 21.437363 14-15 GGTAAGG 935 0.0 21.33693 3 CATGGGG 4405 0.0 20.917753 4 ACGCAGA 21470 0.0 20.419287 7 CGCAGAG 21720 0.0 20.162388 8 >>END_MODULE