FastQCFastQC Report
Thu 26 May 2016
SRR936596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881450
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95760.5089691461372877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73290.38953998246033644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43560.2315235589571873No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19320.10268675755401419No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.10204895160647373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89350.035.5888751
GTATCAA137650.032.9769021
ACGCATA700.008358099527.1659897
GTACATG136250.026.5828271
ATCAACG170400.026.415763
TCAACGC171200.026.295824
TATCAAC173250.026.0614412
CAACGCA173050.025.8780385
TACATGG139800.025.4297732
AACGCAG176400.025.4142156
ACATGGG138600.024.4516983
GAGTACT86950.024.02515612-13
CATGGGG48050.023.0517044
ACGCAGA195400.022.7240587
CGCAGAG197500.022.4601578
GTACTTT94700.022.1540814-15
AGTACTT90750.021.89306612-13
GTGTAAG8450.021.375171
GCAGAGT205500.021.123649
ATGGGAG34250.020.5424945
Produced by FastQC (version 0.11.5)