##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936596_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1881450 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.18926466289298 31.0 31.0 34.0 28.0 34.0 2 31.21439474873103 31.0 31.0 34.0 28.0 34.0 3 31.138376252358555 31.0 31.0 34.0 27.0 34.0 4 34.83669722820165 37.0 35.0 37.0 32.0 37.0 5 34.87981769379999 37.0 35.0 37.0 32.0 37.0 6 34.95053868027319 37.0 35.0 37.0 32.0 37.0 7 34.80712057189933 37.0 35.0 37.0 32.0 37.0 8 34.78307635068697 37.0 35.0 37.0 32.0 37.0 9 36.22413457705493 39.0 35.0 39.0 32.0 39.0 10-11 36.08832841691249 39.0 35.0 39.0 30.5 39.0 12-13 35.97264476866246 38.5 35.0 39.0 30.0 39.0 14-15 36.897091073374256 39.0 36.0 41.0 31.0 41.0 16-17 36.82385633421032 39.0 36.0 41.0 30.5 41.0 18-19 36.72573945627043 39.0 36.0 41.0 30.0 41.0 20-21 36.730225358101464 39.0 36.0 41.0 30.0 41.0 22-23 36.72430120385873 39.0 36.0 41.0 30.0 41.0 24-25 36.814273565601 39.0 36.0 41.0 30.0 41.0 26-27 36.73974009407638 39.0 36.0 41.0 30.0 41.0 28-29 36.619480719657716 39.0 36.0 41.0 30.0 41.0 30-31 36.38659916553722 39.0 35.0 40.5 30.0 41.0 32-33 36.193162985994846 38.5 35.0 40.0 30.0 41.0 34-35 35.98771638895533 38.0 35.0 40.0 29.0 41.0 36-37 35.757475085705174 38.0 35.0 40.0 27.5 41.0 38-39 35.536002019718836 38.0 35.0 40.0 27.0 41.0 40-41 35.27441255414706 38.0 34.0 40.0 26.5 41.0 42-43 34.94195673549656 38.0 34.0 40.0 25.0 41.0 44-45 34.72125089691461 38.0 34.0 40.0 24.5 41.0 46-47 34.46839724680433 38.0 33.0 40.0 23.5 41.0 48-49 34.165631560764304 38.0 33.0 40.0 22.5 41.0 50-51 33.14498684525233 37.0 32.0 39.5 21.0 40.5 52-53 33.22961625342156 37.5 32.5 39.5 20.5 40.0 54-55 33.651195620399164 38.0 33.0 40.0 21.0 41.0 56-57 33.49869409232241 37.5 33.0 40.0 20.0 41.0 58-59 33.25702171197746 37.0 32.5 40.0 18.5 41.0 60-61 32.957444524170185 36.5 32.0 40.0 18.0 41.0 62-63 32.60580217385527 36.0 32.0 39.0 16.0 41.0 64-65 32.23352281485025 35.5 31.0 39.0 13.0 41.0 66-67 31.877303143851815 35.0 31.0 39.0 12.0 40.5 68-69 31.446204523107177 35.0 31.0 38.0 8.0 40.0 70-71 31.05762151532063 35.0 30.0 37.5 7.0 40.0 72-73 30.650555954184274 35.0 30.0 37.0 6.0 39.0 74-75 30.240057402535278 34.5 29.5 36.5 2.0 39.0 76-77 29.789283797071406 34.0 29.0 36.0 2.0 39.0 78-79 29.336347497940416 34.0 29.0 35.5 2.0 37.5 80-81 28.90815514629674 34.0 29.0 35.0 2.0 37.0 82-83 28.527661378192352 34.0 28.0 35.0 2.0 36.5 84-85 28.171011985436763 34.0 27.0 35.0 2.0 36.0 86-87 27.821972946397725 34.0 27.0 35.0 2.0 36.0 88-89 27.465997767679184 33.0 26.0 35.0 2.0 35.0 90-91 27.115252597730475 33.0 25.5 35.0 2.0 35.0 92-93 26.700553296659493 33.0 25.0 35.0 2.0 35.0 94-95 26.19637035265354 33.0 24.5 35.0 2.0 35.0 96-97 25.597553216933747 32.5 21.5 35.0 2.0 35.0 98-99 24.837456748784184 32.0 10.0 35.0 2.0 35.0 100-101 22.292135852666824 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7513498654048121 1101 2 0.6362470102077538 1101 3 0.4836790869610752 1101 4 0.6033747632215736 1101 5 0.6326857464326565 1101 6 0.7026722875463847 1101 7 0.7241107215832869 1101 8 0.6079229989913983 1101 9 0.8521653334763073 1101 10-11 0.9021145612896504 1101 12-13 0.7726404566802358 1101 14-15 1.0733445679208273 1101 16-17 1.0708181268194181 1101 18-19 1.203736285391365 1101 20-21 1.1998023820636519 1101 22-23 1.0675291662293702 1101 24-25 1.0030850147922123 1101 26-27 0.7873795523223208 1101 28-29 0.9589296124673936 1101 30-31 0.7239174466336706 1101 32-33 0.7500543543603868 1101 34-35 1.0583139800428825 1101 36-37 1.0878711514086774 1101 38-39 0.8926162070078547 1101 40-41 0.795536008329151 1101 42-43 0.9264311959623086 1101 44-45 0.9514566247049885 1101 46-47 1.2434792125516836 1101 48-49 1.2222608807447344 1101 50-51 1.1129394726142934 1101 52-53 1.011236636180037 1101 54-55 0.9597788542598522 1101 56-57 1.1258738692803618 1101 58-59 1.1007240635794915 1101 60-61 1.0451750233922965 1101 62-63 1.075589338669424 1101 64-65 1.0078861253062072 1101 66-67 0.9411507908875443 1101 68-69 0.9881447689638208 1101 70-71 0.953634132141655 1101 72-73 1.054998166393652 1101 74-75 0.877689095779921 1101 76-77 0.9293237206312632 1101 78-79 0.8706979136683799 1101 80-81 1.137372217721527 1101 82-83 1.1317067745103557 1101 84-85 1.1342673320263117 1101 86-87 1.0504619381181897 1101 88-89 1.0745032668583754 1101 90-91 1.0561496630450797 1101 92-93 1.078034534022791 1101 94-95 1.3728332482068133 1101 96-97 1.282154640100785 1101 98-99 1.1824877637351463 1101 100-101 1.6508210885488808 1102 1 0.42561891582155553 1102 2 0.45273143647020575 1102 3 0.36517288581089247 1102 4 0.35014368705227383 1102 5 0.3622387071627955 1102 6 0.4009282189508383 1102 7 0.4147915874631565 1102 8 0.3737777053561473 1102 9 0.5733443010875092 1102 10-11 0.6399317637328465 1102 12-13 0.5794406590091086 1102 14-15 0.676813651954653 1102 16-17 0.5712037575024169 1102 18-19 0.6240960455408313 1102 20-21 0.6285279137641879 1102 22-23 0.3680912865691113 1102 24-25 0.7951795727407003 1102 26-27 0.5509414852625198 1102 28-29 0.40592451196329193 1102 30-31 0.5011827756816913 1102 32-33 0.5424200935472072 1102 34-35 0.20836268304488215 1102 36-37 0.22320429194842006 1102 38-39 0.21045536495226003 1102 40-41 0.275976898388258 1102 42-43 0.3114178561421994 1102 44-45 0.4893225562503929 1102 46-47 0.4319354265692752 1102 48-49 0.45101843740960845 1102 50-51 0.41742993637857495 1102 52-53 0.276107494924112 1102 54-55 0.44415852782967136 1102 56-57 0.3792080413006218 1102 58-59 0.24520950566233068 1102 60-61 0.3568336503226064 1102 62-63 0.4441271210802782 1102 64-65 0.40156272171283547 1102 66-67 0.3375100979372654 1102 68-69 0.5069164354051523 1102 70-71 0.29507401838647596 1102 72-73 0.4549970179841729 1102 74-75 0.33609788048603306 1102 76-77 0.31844361092683826 1102 78-79 0.2795481313180801 1102 80-81 0.4456576528095084 1102 82-83 0.4391024532542893 1102 84-85 0.4238128124347078 1102 86-87 0.27421738849567845 1102 88-89 0.2879144188540259 1102 90-91 0.6770936243165373 1102 92-93 0.5358290501587284 1102 94-95 0.7864826429689167 1102 96-97 0.7320324388921371 1102 98-99 0.7635610359139342 1102 100-101 1.3352095839915243 1103 1 0.5075299269540032 1103 2 0.6692676808123146 1103 3 0.6646524759881558 1103 4 0.7937950811586703 1103 5 0.6451564971584389 1103 6 0.574102870386362 1103 7 0.5915218339608401 1103 8 0.5063165517473678 1103 9 0.6944203938521767 1103 10-11 0.7294415966703838 1103 12-13 0.7492041614318623 1103 14-15 1.0582623347177886 1103 16-17 1.1426070761251452 1103 18-19 1.1928344355633484 1103 20-21 1.007826572638173 1103 22-23 0.9365963406656732 1103 24-25 0.9828016069249159 1103 26-27 1.06550578850225 1103 28-29 1.0048182875506981 1103 30-31 0.992216261912354 1103 32-33 1.0327833703108809 1103 34-35 0.991116077274512 1103 36-37 0.8537428635891189 1103 38-39 0.7576119495203741 1103 40-41 1.1361500922318228 1103 42-43 1.0050505631722757 1103 44-45 0.9843800770915294 1103 46-47 1.330950466130325 1103 48-49 1.2004638857933756 1103 50-51 1.1431877478290104 1103 52-53 1.2778961511617055 1103 54-55 1.292089161402373 1103 56-57 1.3846050709645823 1103 58-59 1.2374531063488021 1103 60-61 1.3061573296766156 1103 62-63 1.153065553729526 1103 64-65 1.264379631799713 1103 66-67 1.2344296596697752 1103 68-69 1.3355104904906199 1103 70-71 1.3687425136929896 1103 72-73 1.507842302123688 1103 74-75 1.2886271510132765 1103 76-77 1.294094855536624 1103 78-79 1.0964218541574198 1103 80-81 1.1108165586155323 1103 82-83 1.0947420613711465 1103 84-85 1.1232766448611287 1103 86-87 1.1978296710563896 1103 88-89 1.2113027090269455 1103 90-91 1.2300277983326104 1103 92-93 1.2619194410620587 1103 94-95 1.2520077649652244 1103 96-97 1.1250835232981267 1103 98-99 1.6118704615742203 1103 100-101 2.0317673235219615 1104 1 0.2931300842199889 1104 2 0.3174468795702765 1104 3 0.4772560777210977 1104 4 0.559792043431429 1104 5 0.5153718379324133 1104 6 0.4941646908004742 1104 7 0.4653352374672508 1104 8 0.455309431037179 1104 9 0.47641318716857484 1104 10-11 0.5523056978434866 1104 12-13 0.5809252837921406 1104 14-15 0.5509317186805802 1104 16-17 0.6132173666901508 1104 18-19 0.7752877145440564 1104 20-21 0.8729332827373355 1104 22-23 0.5789582565555165 1104 24-25 0.6579348531452283 1104 26-27 0.4139556526793413 1104 28-29 0.15463211237710794 1104 30-31 0.32369454708967993 1104 32-33 0.37191316351717774 1104 34-35 0.49080586911327373 1104 36-37 0.5715632917769327 1104 38-39 0.35629220736016265 1104 40-41 0.4712587430173727 1104 42-43 0.45368371160411414 1104 44-45 0.5452055123507904 1104 46-47 0.7059341890003026 1104 48-49 0.6402905412717672 1104 50-51 0.44771437369433187 1104 52-53 0.3821871797104066 1104 54-55 0.5107583885013938 1104 56-57 0.7047248103092869 1104 58-59 0.6366233875019773 1104 60-61 0.6952608029522054 1104 62-63 0.4425156607438012 1104 64-65 0.44865496894369983 1104 66-67 0.5887619152350503 1104 68-69 0.4536033940641424 1104 70-71 0.39877639526184794 1104 72-73 0.6491269108214901 1104 74-75 0.6171680112602296 1104 76-77 0.6011412314053466 1104 78-79 0.31541524124341436 1104 80-81 0.4482334195603279 1104 82-83 0.5017453499798705 1104 84-85 0.5583107853557614 1104 86-87 0.5040371776916786 1104 88-89 0.47465133825378203 1104 90-91 0.7582727039051456 1104 92-93 0.5578408070639505 1104 94-95 0.6367244574322903 1104 96-97 0.5334393848497605 1104 98-99 0.6106261095497985 1104 100-101 0.9392939941336849 1105 1 0.41333607732537203 1105 2 0.4010033364610166 1105 3 0.37953541913196887 1105 4 0.615804593385576 1105 5 0.5120341846883036 1105 6 0.36239066954372845 1105 7 0.3960053938791859 1105 8 0.545668504142796 1105 9 0.42228492062974254 1105 10-11 0.6709376006663845 1105 12-13 0.6339091303759687 1105 14-15 0.9887448956351292 1105 16-17 0.7698548283728996 1105 18-19 0.7000853368359898 1105 20-21 0.5911508570494135 1105 22-23 0.5214544173715794 1105 24-25 0.4023413063559218 1105 26-27 0.38582405719008506 1105 28-29 0.5775797652034811 1105 30-31 0.46032424088989643 1105 32-33 0.4244922049544968 1105 34-35 0.30244876632458784 1105 36-37 0.32326518908970314 1105 38-39 0.18461429500097637 1105 40-41 0.05988396705265586 1105 42-43 0.18528608851649864 1105 44-45 0.20870738022318847 1105 46-47 0.563030885710738 1105 48-49 0.5927536612353279 1105 50-51 0.6169829743416315 1105 52-53 0.5742736866696703 1105 54-55 0.5030276794061095 1105 56-57 0.43934109580931135 1105 58-59 0.4227189274841905 1105 60-61 0.38403054341939225 1105 62-63 0.5763026427274838 1105 64-65 0.7253344378632178 1105 66-67 0.660408572061737 1105 68-69 0.7867847596996711 1105 70-71 0.9262556527278676 1105 72-73 0.7371863276633697 1105 74-75 0.6312709202874807 1105 76-77 0.7520255118368446 1105 78-79 0.8397328909467205 1105 80-81 0.9062928214831025 1105 82-83 0.8145792893605481 1105 84-85 1.0000031791963622 1105 86-87 1.0623700220097874 1105 88-89 1.1108243830051414 1105 90-91 1.0176695044525808 1105 92-93 1.0785891843622402 1105 94-95 1.261411404803642 1105 96-97 1.415453479428951 1105 98-99 1.4703815157374471 1105 100-101 1.7243193367261505 1106 1 0.09762710892397664 1106 2 0.07113789540337834 1106 3 0.2069546967356075 1106 4 0.3024525014768713 1106 5 0.3735588368682059 1106 6 0.2384279446054265 1106 7 0.3070059964414682 1106 8 0.3640189200504409 1106 9 0.41978513511797644 1106 10-11 0.5084135081511221 1106 12-13 0.6079531862586265 1106 14-15 0.3342884287650847 1106 16-17 0.2976013751123787 1106 18-19 0.5764631743157551 1106 20-21 0.29235653031300757 1106 22-23 0.10248345708282613 1106 24-25 -0.1403252662019625 1106 26-27 -0.05694861222070102 1106 28-29 -0.01378236974254321 1106 30-31 -0.056139975489820415 1106 32-33 0.029750184415505032 1106 34-35 0.06932761838358203 1106 36-37 0.07667717309586664 1106 38-39 0.04635942113003466 1106 40-41 0.22600634904921435 1106 42-43 0.20081416352951464 1106 44-45 0.12082843438794555 1106 46-47 0.14040514572634066 1106 48-49 0.17255363350150077 1106 50-51 0.1678380092283902 1106 52-53 0.23005000437562728 1106 54-55 0.34973381356999766 1106 56-57 0.4140897205905816 1106 58-59 0.3187816717763141 1106 60-61 0.33629768402192184 1106 62-63 0.18774112550757138 1106 64-65 -0.0033552834617012195 1106 66-67 0.049059376242954755 1106 68-69 0.2877623022830633 1106 70-71 0.0874443065997319 1106 72-73 0.11421561079382414 1106 74-75 0.1856665074165953 1106 76-77 -0.026378840378768587 1106 78-79 0.13513755541729466 1106 80-81 0.39073893306087726 1106 82-83 0.43775152540534634 1106 84-85 0.637298841745519 1106 86-87 0.6482123272694338 1106 88-89 0.6710620309770796 1106 90-91 0.7341023456933762 1106 92-93 0.7218861527955553 1106 94-95 0.9014802006567386 1106 96-97 0.9701942429671497 1106 98-99 0.9377354093472938 1106 100-101 1.4361376881396772 1107 1 0.3489914809641945 1107 2 0.40732437120363585 1107 3 0.46284504217683065 1107 4 0.48079436592060176 1107 5 0.4580823845006705 1107 6 0.2736231944203027 1107 7 0.3473768738625722 1107 8 0.4683127267785636 1107 9 0.4745026656659661 1107 10-11 0.5861967092620688 1107 12-13 0.49532890244960015 1107 14-15 0.3316754488410183 1107 16-17 0.40161995041039233 1107 18-19 0.5366064223314382 1107 20-21 0.41435316861029037 1107 22-23 0.3725188811601967 1107 24-25 0.2661852952210566 1107 26-27 0.2092647532728904 1107 28-29 0.29944462424901985 1107 30-31 0.24828120863877956 1107 32-33 0.3055152590447179 1107 34-35 0.3542089668751842 1107 36-37 0.2703707396041537 1107 38-39 0.35008295128125866 1107 40-41 0.4078917260843582 1107 42-43 0.29524383293752976 1107 44-45 0.22246543531308305 1107 46-47 0.27845359529687386 1107 48-49 0.49352418152876254 1107 50-51 0.500366804181219 1107 52-53 0.5901945045592782 1107 54-55 0.6229331714779036 1107 56-57 0.846190421411805 1107 58-59 0.9689001612394392 1107 60-61 0.7879010237257589 1107 62-63 0.7333211444423142 1107 64-65 0.7626002827441027 1107 66-67 0.7769834094219696 1107 68-69 0.6665697522405409 1107 70-71 0.7018464234019568 1107 72-73 0.6304410069208437 1107 74-75 0.5626940374342517 1107 76-77 0.4891998478206041 1107 78-79 0.8352333830954102 1107 80-81 0.8847901531144196 1107 82-83 0.8493257558867455 1107 84-85 0.8196354902958518 1107 86-87 0.966573087173348 1107 88-89 0.9722047447344302 1107 90-91 0.9316126920381258 1107 92-93 1.1093004161181348 1107 94-95 1.2390916927106268 1107 96-97 1.5091539761915378 1107 98-99 1.6147406458646394 1107 100-101 1.751504784504558 1108 1 0.26567970492314785 1108 2 0.24767171531268772 1108 3 0.3650970389515358 1108 4 0.2827170974263993 1108 5 0.31312976824260375 1108 6 0.34344441694818073 1108 7 0.38939455832628767 1108 8 0.4662710478283074 1108 9 -0.11305988302097347 1108 10-11 0.3131940208643442 1108 12-13 0.25074852775294687 1108 14-15 0.6317999361028583 1108 16-17 0.7478060667983044 1108 18-19 0.5301153182531593 1108 20-21 0.5403304460853491 1108 22-23 0.5648571917062597 1108 24-25 0.40648141432273377 1108 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0.4958453658647173 2311 74-75 0.3565905185623528 2311 76-77 0.48206936161055935 2311 78-79 0.7312373235145806 2311 80-81 0.7995877822132691 2311 82-83 0.8023478941711772 2311 84-85 0.8444853946819961 2311 86-87 0.8668210887531416 2311 88-89 0.7840779400103592 2311 90-91 0.7078357231886976 2311 92-93 0.8528118474645652 2311 94-95 0.6034897888967556 2311 96-97 0.7537774601225635 2311 98-99 0.830563731018394 2311 100-101 0.6182040613051534 2312 1 0.18531716029679046 2312 2 0.10188290509420739 2312 3 0.19809948165738334 2312 4 0.17699418657999644 2312 5 0.06312073456712142 2312 6 0.11767092784330657 2312 7 0.21619602807948013 2312 8 0.15554952042477765 2312 9 0.42516598570887965 2312 10-11 0.18524736497614924 2312 12-13 -1.9458796688809343E-4 2312 14-15 0.14774024364014338 2312 16-17 0.017399721751125696 2312 18-19 0.14017361901364467 2312 20-21 0.11172190570017904 2312 22-23 0.0470607219633834 2312 24-25 0.07458508065283098 2312 26-27 0.08709196981065048 2312 28-29 -0.03321518103832233 2312 30-31 0.07421373974316481 2312 32-33 0.06083042613571621 2312 34-35 0.1195539973791 2312 36-37 0.09274948454018528 2312 38-39 0.11915190133809972 2312 40-41 0.2805578963618558 2312 42-43 -0.06699416165022853 2312 44-45 -0.13438233333754113 2312 46-47 -0.12332339786576796 2312 48-49 -0.07106345517841106 2312 50-51 -0.14746809838239017 2312 52-53 -0.3414068709190943 2312 54-55 -0.584582520824867 2312 56-57 -0.15747245027959877 2312 58-59 0.01897842259634075 2312 60-61 -0.1872885248803513 2312 62-63 -0.382583724975305 2312 64-65 -0.36638183923953704 2312 66-67 -0.29378106992984243 2312 68-69 -0.4087372884793865 2312 70-71 -0.3887065701449828 2312 72-73 -0.48790577695772086 2312 74-75 -0.6106348524153908 2312 76-77 -0.4767944917416109 2312 78-79 -0.48414701113366476 2312 80-81 -0.6106702878713115 2312 82-83 -0.6304293721336194 2312 84-85 -0.7999762853362427 2312 86-87 -0.9187790288856412 2312 88-89 -0.6997440631309395 2312 90-91 -0.8296391293898715 2312 92-93 -0.5975197308771136 2312 94-95 -0.4982473811232566 2312 96-97 -0.6226743772375549 2312 98-99 -0.5360515752367085 2312 100-101 -0.7436504171735585 2313 1 -0.1761522755961238 2313 2 -0.02764835572636315 2313 3 -0.03915926555943372 2313 4 0.08481920237147023 2313 5 -0.07465199623495522 2313 6 0.048645665683871187 2313 7 0.15266263095069377 2313 8 0.10548714148399796 2313 9 -0.03528870378145399 2313 10-11 0.2365899811772536 2313 12-13 -0.022244940205922603 2313 14-15 0.004065384294428043 2313 16-17 0.07717136446302675 2313 18-19 -0.17453862992292812 2313 20-21 -0.06886394744857682 2313 22-23 -0.19439811108349403 2313 24-25 -0.23703248555069223 2313 26-27 -0.40768254789363567 2313 28-29 -0.3313747137366434 2313 30-31 -0.2012720447835008 2313 32-33 -0.1899129797439585 2313 34-35 -0.465564926576306 2313 36-37 -0.28663868245281066 2313 38-39 -0.21062926872084375 2313 40-41 -0.01700998073957294 2313 42-43 -0.04608672381407075 2313 44-45 -0.05338073425418344 2313 46-47 -0.31906486784727406 2313 48-49 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68-69 -1.2717089567288298 2314 70-71 -1.319232844282368 2314 72-73 -1.3176588633774706 2314 74-75 -1.270803418417291 2314 76-77 -1.1503407088989412 2314 78-79 -0.9716201038845327 2314 80-81 -1.0282343904354185 2314 82-83 -1.0562935696644828 2314 84-85 -1.1150150634305795 2314 86-87 -1.0536888104620914 2314 88-89 -0.9623303246060892 2314 90-91 -0.9271217188169061 2314 92-93 -1.0054898163471968 2314 94-95 -1.127822404864947 2314 96-97 -1.0543537061391035 2314 98-99 -0.9422687325649726 2314 100-101 -1.412957951203314 2315 1 -0.8151972429953886 2315 2 -1.1779524858522947 2315 3 -1.2267976376841823 2315 4 -0.8888494348191784 2315 5 -0.7933607469143595 2315 6 -0.7285224877945495 2315 7 -0.45304265504809393 2315 8 -0.28169327792912924 2315 9 -0.8124883352787791 2315 10-11 -0.860020124735783 2315 12-13 -0.9068303904360278 2315 14-15 -0.8039058468948355 2315 16-17 -0.9525076856562791 2315 18-19 -1.0967090970357418 2315 20-21 -1.2769097815426278 2315 22-23 -0.9529392780366166 2315 24-25 -1.2983573061784384 2315 26-27 -1.326488277102925 2315 28-29 -1.3454579793922363 2315 30-31 -0.968996136800051 2315 32-33 -1.1516181335350666 2315 34-35 -0.8814748092052653 2315 36-37 -1.0283924907684607 2315 38-39 -1.129819292077535 2315 40-41 -1.3537013628974108 2315 42-43 -1.4383933386049534 2315 44-45 -1.483404967082393 2315 46-47 -2.0006382126805775 2315 48-49 -2.5584605745199767 2315 50-51 -2.2966450531149007 2315 52-53 -1.9939274470507868 2315 54-55 -2.000477582553259 2315 56-57 -2.146412779497709 2315 58-59 -2.3645092317246466 2315 60-61 -2.553286467267185 2315 62-63 -2.5374191159218107 2315 64-65 -1.9818653783341844 2315 66-67 -1.9774230452384849 2315 68-69 -2.0700027205781524 2315 70-71 -1.8244061586223488 2315 72-73 -1.777000427163479 2315 74-75 -1.6340402022096292 2315 76-77 -1.5611566316593048 2315 78-79 -1.6526140893229666 2315 80-81 -1.9182834565955922 2315 82-83 -1.768289454438147 2315 84-85 -1.7364474787518844 2315 86-87 -1.8210424033712371 2315 88-89 -1.9469148450239437 2315 90-91 -1.7087543557273186 2315 92-93 -1.5489086197659994 2315 94-95 -1.762136270012146 2315 96-97 -1.7623348710647129 2315 98-99 -1.795053632849875 2315 100-101 -2.072096919231168 2316 1 -5.5108222244916725 2316 2 -5.3379541141792615 2316 3 -6.328597679132503 2316 4 -7.802007797018309 2316 5 -8.608745476167993 2316 6 -9.429198983634915 2316 7 -11.540802077140047 2316 8 -11.295093668727603 2316 9 -11.946795704198454 2316 10-11 -11.595644588128035 2316 12-13 -9.906036581039096 2316 14-15 -10.098732948176774 2316 16-17 -9.006142555538151 2316 18-19 -9.148421430872375 2316 20-21 -10.389915673125657 2316 22-23 -10.00932194967708 2316 24-25 -9.815556509778524 2316 26-27 -8.133733221887837 2316 28-29 -6.3391408109048015 2316 30-31 -8.329357847431545 2316 32-33 -6.003799351523639 2316 34-35 -4.728822856693199 2316 36-37 -5.361174413378521 2316 38-39 -4.253681313571455 2316 40-41 -4.03179407391378 2316 42-43 -4.157040642983677 2316 44-45 -4.245804306869829 2316 46-47 -4.412157145682329 2316 48-49 -4.838046165306604 2316 50-51 -4.7996167498316 2316 52-53 -4.737308667999951 2316 54-55 -4.165421553302586 2316 56-57 -4.8108775040143925 2316 58-59 -4.846497715012092 2316 60-61 -4.963641516561079 2316 62-63 -4.74248284271815 2316 64-65 -5.38606794220901 2316 66-67 -4.810576892531021 2316 68-69 -4.793893977942631 2316 70-71 -4.646376360238833 2316 72-73 -3.880980206303132 2316 74-75 -4.072792903752099 2316 76-77 -4.340346242933251 2316 78-79 -4.5972546731522 2316 80-81 -5.304914892187863 2316 82-83 -4.804279181175275 2316 84-85 -4.769713458560631 2316 86-87 -5.555525187514295 2316 88-89 -6.9512502655763875 2316 90-91 -6.104406352648301 2316 92-93 -5.343073288031388 2316 94-95 -4.925777579187031 2316 96-97 -6.329727253455101 2316 98-99 -7.574013798051045 2316 100-101 -8.019798017611725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4705.0 3 1825.0 4 1914.0 5 1988.0 6 2376.0 7 2841.0 8 3495.0 9 4652.0 10 5860.0 11 7409.0 12 9413.0 13 11362.0 14 13040.0 15 16366.0 16 20311.0 17 24767.0 18 19213.0 19 14297.0 20 14446.0 21 15173.0 22 17110.0 23 18977.0 24 21803.0 25 25406.0 26 29293.0 27 34590.0 28 40725.0 29 48251.0 30 58100.0 31 70029.0 32 86790.0 33 107842.0 34 139444.0 35 186783.0 36 249193.0 37 305710.0 38 217732.0 39 28216.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02275920979428 18.658296979232155 14.267881058529305 22.05106275244426 2 15.79031873045848 20.387829218615675 39.5406849055069 24.281167145418944 3 19.121773124369405 25.737393110824524 29.710060951947582 25.43077281285849 4 12.61689993493155 16.630889651564832 37.02171500019138 33.73049541331224 5 13.564140991786767 37.97826568895405 33.05569570050649 15.401897618752688 6 31.20359953809543 36.05689379810177 17.5022914464716 15.237215217331205 7 28.793592606264205 33.796789282146804 20.026828674129476 17.382789437459508 8 27.711596845650327 32.928270930750124 19.56254111380727 19.79759110979228 9 28.04784322026912 16.154692653370144 20.14077667579187 35.65668745056887 10-11 25.40382131189476 26.596738158714157 26.28791250985854 21.711528019532544 12-13 26.83912412609071 24.374153605990728 27.01070251433852 21.776019753580044 14-15 23.60633169086413 25.040153333756955 26.20549733104293 25.14801764433598 16-17 22.25538255693125 28.70429360155624 26.5110937627963 22.529230078716207 18-19 22.15767928324988 26.309686634533165 29.901573421367218 21.631060660849737 20-21 23.313854818839182 25.337290169618566 29.849093564647895 21.499761446894357 22-23 22.801232286414365 25.47582856536867 29.844433275560494 21.878505872656472 24-25 22.465650742007767 25.877371354752277 29.94205348622707 21.714924417012885 26-27 22.11589886666787 26.133225801992765 29.825300557894142 21.925574773445216 28-29 21.851603110890107 26.15638486993087 30.02716434388183 21.964847675297193 30-31 21.868449138741127 25.910131219198995 30.016391769673312 22.205027872386566 32-33 21.72273555284228 26.110795428873956 29.753195551768552 22.41327346651521 34-35 22.60089164072205 26.009873217343138 29.430761764379127 21.95847337755568 36-37 22.739404853435147 25.596899278371037 29.357512895533226 22.306182972660594 38-39 22.256155983201857 25.92615823432935 29.437119790015252 22.38056599245354 40-41 22.343953391584996 25.927392542802423 29.610838231784488 22.117815833828093 42-43 22.368846263828665 26.0989051874703 28.97944529073284 22.552803257968193 44-45 22.292895115331994 26.24391555820601 28.985116703287595 22.478072623174402 46-47 22.643020220963905 26.499271700882066 28.268476487538656 22.589231590615373 48-49 22.763609923406687 26.76593687622264 27.68366942068814 22.786783779682533 50-51 22.723380620060276 26.94439867547557 27.591725442881216 22.740495261582943 52-53 23.078114876475393 26.8666882458023 27.432662200954905 22.6225346767674 54-55 22.401145077929506 26.769624820084996 27.682700096309006 23.146530005676492 56-57 22.628225878035728 27.178539024666375 27.247504102026852 22.94573099527104 58-59 22.885809474099357 26.499854893902462 27.485167818125188 23.129167813872993 60-61 22.653316412971982 26.80392534665701 27.99008697154323 22.55267126882778 62-63 22.657689668497042 26.819348985144583 27.891741314052666 22.631220032305713 64-65 22.416978484565014 26.864263542818268 27.758737987924142 22.960019984692575 66-67 22.409655588089755 27.149485048412416 27.3756417295485 23.065217633949334 68-69 22.58609417605256 26.767727772846385 27.7402105912181 22.90596745988296 70-71 22.448954100547734 27.021305999006056 27.512351207481682 23.01738869296453 72-73 22.612346036051314 26.99348392725092 27.500968878402972 22.893201158294797 74-75 22.35549876949256 27.518446979734833 27.248822452621013 22.877231798151595 76-77 22.54965053962806 27.131139413861398 27.433908488246633 22.885301558263908 78-79 22.534919629498006 27.204284583849773 27.345985887283803 22.914809899368418 80-81 22.425032698482646 27.642756352515825 27.085772485539177 22.846438463462356 82-83 22.58739303598413 27.563668139539704 26.895028428947825 22.953910395528336 84-85 22.3774950721105 27.385724388462002 26.915697942498166 23.321082596929337 86-87 22.635282199233664 27.24042331878226 26.883830768159566 23.240463713824514 88-89 22.49262391843603 27.186793585054687 26.71919439529419 23.601388101215093 90-91 22.360728810897136 27.188408678183112 27.13307573304857 23.31778677787118 92-93 22.50199798699654 26.920187097163428 27.32472796945253 23.253086946387505 94-95 22.186007360686112 26.68348385255132 27.470530278243714 23.65997850851885 96-97 22.328501889034037 26.565280311919853 27.40929247934554 23.696925319700572 98-99 22.085932295927943 26.762975142992783 27.02596809529202 24.12512446578726 100-101 21.96725503272397 26.623114447905454 27.31360973168286 24.09602078768772 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 553.0 1 417.5 2 375.0 3 497.5 4 494.5 5 484.5 6 718.0 7 1436.0 8 2366.0 9 2811.0 10 2523.5 11 2151.0 12 2150.0 13 2353.0 14 2579.0 15 2722.5 16 2843.5 17 2988.5 18 3100.0 19 3300.5 20 3593.5 21 4049.0 22 4769.5 23 5581.5 24 6538.5 25 7857.5 26 9774.5 27 12039.5 28 14032.5 29 16654.5 30 20410.0 31 24817.0 32 30646.5 33 34773.0 34 37776.0 35 40557.5 36 44039.0 37 49128.5 38 50903.0 39 50506.5 40 51075.0 41 53902.0 42 56882.0 43 59103.5 44 60971.5 45 63407.0 46 63600.0 47 63744.0 48 70686.0 49 72164.5 50 66251.0 51 62146.0 52 59717.0 53 61045.0 54 62594.5 55 59598.5 56 55108.0 57 50817.0 58 47813.0 59 44078.5 60 38291.0 61 32829.0 62 28472.0 63 22519.5 64 16502.5 65 13027.5 66 9872.0 67 7655.5 68 5527.0 69 3613.0 70 2748.5 71 2242.5 72 1925.5 73 1725.0 74 1429.5 75 1144.5 76 805.5 77 449.0 78 278.0 79 202.0 80 138.5 81 97.0 82 60.5 83 44.0 84 35.0 85 21.5 86 14.5 87 8.0 88 4.5 89 4.5 90 5.5 91 4.5 92 2.5 93 2.0 94 4.0 95 2.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07079646017699115 2 0.02758510723112493 3 0.015732546705998034 4 0.018815275452443592 5 0.02450237848467937 6 0.028807568630577478 7 0.07292248000212602 8 0.05309734513274337 9 0.0695739987775386 10-11 0.05851869568683728 12-13 0.03598288554040766 14-15 0.044991894549416676 16-17 0.05442610752345266 18-19 0.051130776794493606 20-21 0.016875282362008026 22-23 0.012304339737968057 24-25 0.021525950729490553 26-27 0.023811422041510537 28-29 0.006564086210103909 30-31 9.567089213106912E-4 32-33 0.008663530787424593 34-35 0.010576948630045975 36-37 0.004331765393712297 38-39 0.00906215950463738 40-41 0.003879986180871137 42-43 0.0017008158601078955 44-45 6.378059475404608E-4 46-47 6.112306997262749E-4 48-49 0.0010364346647532487 50-51 0.0010630099125674348 52-53 1.594514868851152E-4 54-55 3.189029737702304E-4 56-57 0.003348481224587419 58-59 0.003614233702729278 60-61 0.004650668367482526 62-63 0.002604374285790215 64-65 5.315049562837174E-4 66-67 5.846554519120891E-4 68-69 0.004252039650269739 70-71 0.0029232772595604454 72-73 0.02173855271200404 74-75 0.016768981370751283 76-77 0.025432512158175873 78-79 0.020223763586595445 80-81 0.013101597172393632 82-83 0.004942996093438571 84-85 0.0035345079592867205 86-87 0.0016476653644795238 88-89 0.0016476653644795238 90-91 0.005926280262563448 92-93 0.00964681495654947 94-95 0.01272954370299503 96-97 0.010497222886603418 98-99 0.021844853703260784 100-101 0.017885141778947088 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1881450.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.06341874835052 #Duplication Level Percentage of deduplicated Percentage of total 1 71.81255825343042 31.643068257112205 2 14.176921286694242 12.493672384360252 3 5.431498354168739 7.179911592321414 4 2.709718273856469 4.775978039639806 5 1.5091761736963325 3.324973085330744 6 0.9492748635112773 2.5096977490908423 7 0.6247523280140401 1.927010641030264 8 0.4477563559823847 1.5783740648709859 9 0.315514321794312 1.2512375714092128 >10 1.7193250723144013 14.224860410023584 >50 0.175595388834026 5.389580098775293 >100 0.11926077343927198 9.570193070408594 >500 0.006577773665689505 1.9914636424572645 >1k 0.001827159351580418 1.2326216895946709 >5k 2.4362124687738907E-4 0.9073577035749144 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9576 0.5089691461372877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7329 0.38953998246033644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4356 0.2315235589571873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1932 0.10268675755401419 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1920 0.10204895160647373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3150495628371736E-5 0.0 0.0 0.0 0.0 3 5.3150495628371736E-5 0.0 0.0 0.0 0.0 4 5.3150495628371736E-5 0.0 0.0 0.0 0.0 5 1.0630099125674347E-4 0.0 0.0 0.0 0.0 6 1.0630099125674347E-4 0.0 0.0 0.0 0.0 7 1.0630099125674347E-4 0.0 0.0 0.0 0.0 8 1.0630099125674347E-4 0.0 0.0 0.0 0.0 9 1.0630099125674347E-4 0.0 0.0 0.0 0.0 10-11 1.0630099125674347E-4 0.0 0.0 2.6575247814185868E-5 0.0 12-13 1.0630099125674347E-4 0.0 0.0 5.3150495628371736E-5 0.0 14-15 1.0630099125674347E-4 0.0 0.0 5.3150495628371736E-5 0.0 16-17 1.0630099125674347E-4 0.0 0.0 5.3150495628371736E-5 0.0 18-19 1.594514868851152E-4 0.0 0.0 7.97257434425576E-5 0.0 20-21 2.1260198251348694E-4 0.0 0.0 1.594514868851152E-4 0.0 22-23 2.1260198251348694E-4 0.0 0.0 3.189029737702304E-4 0.0 24-25 2.1260198251348694E-4 0.0 0.0 4.5177921284115976E-4 0.0 26-27 2.657524781418587E-4 0.0 0.0 7.706821866113902E-4 0.0 28-29 2.657524781418587E-4 0.0 0.0 0.0018868425948071967 0.0 30-31 2.657524781418587E-4 0.0 0.0 0.004517792128411597 0.0 32-33 2.657524781418587E-4 0.0 0.0 0.00823832682239762 0.0 34-35 2.657524781418587E-4 0.0 0.0 0.012649817959552473 0.0 36-37 2.657524781418587E-4 0.0 0.0 0.017194185335778257 0.0 38-39 3.189029737702304E-4 0.0 0.0 0.02128677349916288 0.0 40-41 3.189029737702304E-4 0.0 0.0 0.02476813096282123 0.0 42-43 3.189029737702304E-4 0.0 0.0 0.029737702304073986 0.0 44-45 4.252039650269739E-4 0.0 0.0 0.03454782215844163 0.0 46-47 5.315049562837173E-4 0.0 0.0 0.042865874724281806 0.0 48-49 5.84655451912089E-4 0.0 0.0 0.05184830848547663 0.0 50-51 5.84655451912089E-4 0.0 0.0 0.06035238778601611 0.0 52-53 5.84655451912089E-4 5.3150495628371736E-5 0.0 0.07534082755321694 0.0 54-55 5.84655451912089E-4 5.3150495628371736E-5 0.0 0.1047596268835207 0.0 56-57 7.97257434425576E-4 5.3150495628371736E-5 0.0 0.15387068484413619 0.0 58-59 7.97257434425576E-4 5.3150495628371736E-5 0.0 0.20300831805256583 0.0 60-61 8.504079300539478E-4 5.3150495628371736E-5 0.0 0.2518536235350395 0.0 62-63 8.504079300539478E-4 5.3150495628371736E-5 0.0 0.3083791756358128 0.0 64-65 9.035584256823195E-4 5.3150495628371736E-5 0.0 0.3755348268622605 0.0 66-67 0.0010630099125674348 5.3150495628371736E-5 0.0 0.48433389141353744 0.0 68-69 0.0010630099125674348 5.3150495628371736E-5 0.0 0.6200271067527705 0.0 70-71 0.0010895851603816206 5.3150495628371736E-5 0.0 0.7363203911876478 0.0 72-73 0.001169310903824178 5.3150495628371736E-5 0.0 0.8597624172845412 0.0 74-75 0.0012490366472667357 5.3150495628371736E-5 0.0 0.9923197533817003 0.0 76-77 0.0012756118950809216 5.3150495628371736E-5 0.0 1.137819235164368 0.0 78-79 0.0012756118950809216 5.3150495628371736E-5 0.0 1.2563979909112652 0.0 80-81 0.0013287623907092933 5.3150495628371736E-5 0.0 1.3887958755215393 0.0 82-83 0.0013287623907092933 5.3150495628371736E-5 0.0 1.5363416513858992 0.0 84-85 0.001381912886337665 5.3150495628371736E-5 0.0 1.7708947885939037 0.0 86-87 0.0014350633819660368 5.3150495628371736E-5 0.0 2.0869010603523876 2.6575247814185868E-5 88-89 0.0014882138775944086 5.3150495628371736E-5 0.0 2.439634324590077 5.3150495628371736E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8935 0.0 35.588875 1 GTATCAA 13765 0.0 32.976902 1 ACGCATA 70 0.0083580995 27.165989 7 GTACATG 13625 0.0 26.582827 1 ATCAACG 17040 0.0 26.41576 3 TCAACGC 17120 0.0 26.29582 4 TATCAAC 17325 0.0 26.061441 2 CAACGCA 17305 0.0 25.878038 5 TACATGG 13980 0.0 25.429773 2 AACGCAG 17640 0.0 25.414215 6 ACATGGG 13860 0.0 24.451698 3 GAGTACT 8695 0.0 24.025156 12-13 CATGGGG 4805 0.0 23.051704 4 ACGCAGA 19540 0.0 22.724058 7 CGCAGAG 19750 0.0 22.460157 8 GTACTTT 9470 0.0 22.15408 14-15 AGTACTT 9075 0.0 21.893066 12-13 GTGTAAG 845 0.0 21.37517 1 GCAGAGT 20550 0.0 21.12364 9 ATGGGAG 3425 0.0 20.542494 5 >>END_MODULE