FastQCFastQC Report
Thu 26 May 2016
SRR936596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881450
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108670.5775864359935157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94180.500571367828005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57100.3034893300380026No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.1293151558638284No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23010.12229929044088336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.033.3583341
GTATCAA149950.032.670381
GTAAGGT7900.028.8545114
TAAGGTG8750.027.1369825
GGTAAGG8050.026.5491623
ATCAACG190150.025.676213
TCAACGC189900.025.6329424
TATCAAC192450.025.3747482
CAACGCA194050.025.1092225
AACGCAG197050.024.7028456
AGGTAAG7550.024.5382842
GTACATG131450.024.5081831
TACATGG137300.023.2155272
AGTACTT110450.022.87612-13
GAGTACT107650.022.78717412-13
TACCTGG12050.022.4705832
ACATGGG134900.022.4265083
ACGCAGA220300.022.0742037
CGCAGAG222000.021.840998
GTACTTT114950.021.23676514-15
Produced by FastQC (version 0.11.5)