##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936596_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1881450 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801941587605306 31.0 31.0 34.0 30.0 34.0 2 31.943074756172102 31.0 31.0 34.0 30.0 34.0 3 32.01678758404422 31.0 31.0 34.0 30.0 34.0 4 35.75755720322092 37.0 35.0 37.0 35.0 37.0 5 35.57793510324484 37.0 35.0 37.0 33.0 37.0 6 35.670990459486035 37.0 35.0 37.0 33.0 37.0 7 35.60810491907837 37.0 35.0 37.0 33.0 37.0 8 35.57387865741848 37.0 35.0 37.0 33.0 37.0 9 37.169881740147225 39.0 37.0 39.0 33.0 39.0 10-11 37.057231390682716 39.0 37.0 39.0 33.0 39.0 12-13 36.94690743841186 39.0 37.0 39.0 33.0 39.0 14-15 38.023314464907386 40.0 37.0 41.0 33.0 41.0 16-17 37.927473491190305 40.0 37.0 41.0 33.0 41.0 18-19 38.09081134231577 40.0 37.0 41.0 33.0 41.0 20-21 38.123902575141514 40.0 37.0 41.0 33.0 41.0 22-23 38.042189003162456 40.0 37.0 41.0 33.0 41.0 24-25 37.89115921230966 40.0 37.0 41.0 33.0 41.0 26-27 37.677606898934336 39.5 37.0 41.0 32.0 41.0 28-29 37.57736878471392 39.0 36.5 41.0 32.0 41.0 30-31 37.50095378564405 39.0 36.5 41.0 32.0 41.0 32-33 37.30518031305642 39.0 36.0 41.0 32.0 41.0 34-35 37.11272130537617 39.0 35.5 41.0 31.0 41.0 36-37 36.875741848042736 39.0 35.0 41.0 31.0 41.0 38-39 36.68708177203753 39.0 35.0 41.0 30.5 41.0 40-41 36.44961279863935 39.0 35.0 40.5 30.0 41.0 42-43 36.11172845411784 39.0 35.0 40.0 30.0 41.0 44-45 35.76179037444524 38.0 35.0 40.0 28.5 41.0 46-47 35.28760105237981 38.0 34.5 40.0 26.5 41.0 48-49 34.823493847830136 38.0 34.0 40.0 25.5 41.0 50-51 34.49957426453002 38.0 34.0 40.0 24.0 41.0 52-53 34.11742565574424 38.0 33.0 40.0 23.0 41.0 54-55 33.89023173616094 37.5 33.0 40.0 23.0 41.0 56-57 33.57491349756836 37.0 33.0 40.0 22.0 41.0 58-59 33.19339977145287 37.0 32.5 40.0 21.0 41.0 60-61 32.76638736081214 36.0 32.0 39.0 19.0 40.5 62-63 32.43807063700869 36.0 31.0 39.0 18.5 40.0 64-65 32.00909272103962 35.0 31.0 38.5 17.5 40.0 66-67 31.585419490286746 35.0 31.0 38.0 15.0 40.0 68-69 31.23897711871163 35.0 30.0 38.0 13.0 40.0 70-71 30.878958250285685 34.5 30.0 37.0 9.5 39.0 72-73 30.440779717770866 34.0 29.5 36.5 7.5 39.0 74-75 29.94653883972468 34.0 29.0 36.0 7.0 39.0 76-77 28.456122405591433 32.5 27.0 34.5 3.5 36.5 78-79 29.229594461718357 34.0 29.0 35.0 2.0 37.0 80-81 29.034707539397804 34.0 29.0 35.0 2.0 37.0 82-83 28.751972946397725 34.0 29.0 35.0 2.0 36.0 84-85 28.38380717000186 33.5 28.0 35.0 2.0 36.0 86-87 28.004546227643573 33.0 27.0 35.0 2.0 35.5 88-89 27.547998086582158 33.0 26.5 35.0 2.0 35.0 90-91 27.098739270243694 33.0 26.0 35.0 2.0 35.0 92-93 26.49930665178453 32.0 25.0 34.0 2.0 35.0 94-95 25.975836455924952 32.0 24.0 34.0 2.0 35.0 96-97 25.268429934359137 32.0 21.5 34.0 2.0 35.0 98-99 24.406173961572193 31.5 10.0 34.0 2.0 35.0 100-101 21.325933455579474 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29763935091301974 1101 2 0.31439844642498116 1101 3 0.24782437179354844 1101 4 0.2617142313263301 1101 5 0.21146301634733078 1101 6 0.17819294792953855 1101 7 0.23586583139837813 1101 8 0.23101575950092013 1101 9 0.2669332362115995 1101 10-11 0.34688551463352013 1101 12-13 0.33808695364397323 1101 14-15 0.571721695268451 1101 16-17 0.5526154889310249 1101 18-19 0.41683060607105915 1101 20-21 0.48224939475728235 1101 22-23 0.3213579046903732 1101 24-25 0.27905104473226316 1101 26-27 0.3311086341193814 1101 28-29 0.45639940401856194 1101 30-31 0.4611104775285355 1101 32-33 0.42974322729490666 1101 34-35 0.45018534361074813 1101 36-37 0.4299462790013422 1101 38-39 0.5260887665945191 1101 40-41 0.44936586735517636 1101 42-43 0.6474548061368637 1101 44-45 0.6108913545866699 1101 46-47 0.4040058037074914 1101 48-49 0.439775574333531 1101 50-51 0.4305259066056024 1101 52-53 0.5021386316657086 1101 54-55 0.39235276860858903 1101 56-57 0.3658273806047916 1101 58-59 0.18455929096543855 1101 60-61 0.27624209913847153 1101 62-63 0.4316806833054514 1101 64-65 0.39044263529027035 1101 66-67 0.1058426208929042 1101 68-69 0.07900719970538006 1101 70-71 0.156986024478325 1101 72-73 0.41749020859906594 1101 74-75 0.42732447520719674 1101 76-77 0.29722670756469327 1101 78-79 0.4055274102155515 1101 80-81 0.49253251470307546 1101 82-83 0.4062302706655281 1101 84-85 0.45023801735807467 1101 86-87 0.46577313240247165 1101 88-89 0.422962625941949 1101 90-91 0.45459394243448514 1101 92-93 0.5052244087007196 1101 94-95 0.4670519812144107 1101 96-97 0.5561252566219039 1101 98-99 0.5392093635260267 1101 100-101 1.3576428008489216 1102 1 0.2158130875190416 1102 2 0.1458158499442348 1102 3 0.1277435446463997 1102 4 0.15744021664860952 1102 5 0.12169576240842161 1102 6 0.09210086532549155 1102 7 0.15038632085049386 1102 8 0.1945837737913152 1102 9 0.24071243778512752 1102 10-11 0.23673764647301 1102 12-13 0.2589698926117521 1102 14-15 0.33751023758708243 1102 16-17 0.2977552373145187 1102 18-19 0.1685345586354785 1102 20-21 0.14605095377535093 1102 22-23 0.1879597036216225 1102 24-25 0.07752162430843157 1102 26-27 0.2883298590233707 1102 28-29 0.19479015869584515 1102 30-31 0.20335466801208923 1102 32-33 0.31240161775725994 1102 34-35 0.24532462219035267 1102 36-37 0.06365977067250128 1102 38-39 0.12648494176454506 1102 40-41 0.19734792919911825 1102 42-43 0.13300951140540462 1102 44-45 0.25886730898020005 1102 46-47 0.18902942179243354 1102 48-49 0.24768033777944964 1102 50-51 0.32173277012705626 1102 52-53 0.2969861098368014 1102 54-55 0.19667710450165288 1102 56-57 0.10413813812178319 1102 58-59 0.08269706669464227 1102 60-61 0.17240265304768343 1102 62-63 0.2448935780646906 1102 64-65 0.10862464566458385 1102 66-67 0.12901230413547182 1102 68-69 0.17728716940824896 1102 70-71 0.1305027883856944 1102 72-73 0.23425938821187842 1102 74-75 -0.037253878422536246 1102 76-77 -0.31597827882926666 1102 78-79 -0.04279742575764445 1102 80-81 0.039833152122536575 1102 82-83 -0.041292114184237505 1102 84-85 0.03632435791472943 1102 86-87 -0.005183722375409872 1102 88-89 0.01099591206869377 1102 90-91 -0.18278101795843682 1102 92-93 0.03885897332041566 1102 94-95 -0.16339193646968653 1102 96-97 0.16322722964158842 1102 98-99 0.2277357871231267 1102 100-101 0.7211408926621736 1103 1 0.2586246743144507 1103 2 0.3176066476197619 1103 3 0.23100226772312027 1103 4 0.217057545730043 1103 5 0.21594175908111168 1103 6 0.18500492789588208 1103 7 0.1854028715394449 1103 8 0.15438433723191736 1103 9 0.295243851770536 1103 10-11 0.25350071151716946 1103 12-13 0.2613029993432363 1103 14-15 0.327124011073451 1103 16-17 0.2867303673412138 1103 18-19 0.26477628455364766 1103 20-21 0.28877173148096347 1103 22-23 0.3214735608462931 1103 24-25 0.3786997182064411 1103 26-27 0.36198547539890313 1103 28-29 0.22536701359019418 1103 30-31 0.32021278461741787 1103 32-33 0.24046773768384355 1103 34-35 0.2540224461055658 1103 36-37 0.27547323526654566 1103 38-39 0.25458510247340627 1103 40-41 0.3208182404854014 1103 42-43 0.494976412047734 1103 44-45 0.31230906439364503 1103 46-47 0.4456956574778843 1103 48-49 0.23404003470538015 1103 50-51 0.3721631284441713 1103 52-53 0.43725888054434847 1103 54-55 0.24384858822521238 1103 56-57 0.05467041676326545 1103 58-59 0.3546157445789433 1103 60-61 0.3636530103748896 1103 62-63 0.3698800678135612 1103 64-65 0.23481973470092754 1103 66-67 -0.041986060492824606 1103 68-69 0.14955075011470242 1103 70-71 0.16598373817402745 1103 72-73 0.1592668882952104 1103 74-75 0.08214113505740883 1103 76-77 0.13833358726217782 1103 78-79 -0.0934568270237186 1103 80-81 -0.03208380857096671 1103 82-83 0.12070539285877402 1103 84-85 0.3337705921658163 1103 86-87 0.39613639330264405 1103 88-89 0.29388365122539284 1103 90-91 0.3444809011133927 1103 92-93 0.477421674522148 1103 94-95 0.6076714288808347 1103 96-97 0.6355592454518657 1103 98-99 0.41867553493850806 1103 100-101 0.7734702619783995 1104 1 0.19663382168443988 1104 2 0.1736074661268674 1104 3 0.19355707577365422 1104 4 0.11560967301895886 1104 5 0.10905367987295023 1104 6 -0.034598820142690556 1104 7 0.03427594109696486 1104 8 0.08842890363682443 1104 9 0.13518373071394052 1104 10-11 0.11111981611664845 1104 12-13 0.08619137216819439 1104 14-15 0.13010713766000492 1104 16-17 0.23741204600477772 1104 18-19 0.17724919161639008 1104 20-21 0.18814839910121606 1104 22-23 0.18446097604810774 1104 24-25 0.18389276371151908 1104 26-27 0.12872213581951542 1104 28-29 0.1483095717869034 1104 30-31 0.06963233839027083 1104 32-33 0.08218427479544488 1104 34-35 0.09432683220763494 1104 36-37 -0.08341171471049336 1104 38-39 0.2534898213076673 1104 40-41 0.1881671723788756 1104 42-43 0.0345239304578584 1104 44-45 0.1183584503373396 1104 46-47 0.3096281517576358 1104 48-49 0.30370552271509865 1104 50-51 0.34990870948617214 1104 52-53 0.280471153927742 1104 54-55 0.3980995964017353 1104 56-57 0.2888322036427198 1104 58-59 0.1926708701277775 1104 60-61 0.3880638034067161 1104 62-63 0.1703571448381993 1104 64-65 0.3473908575891116 1104 66-67 0.16348536792777324 1104 68-69 0.13261650167567396 1104 70-71 0.222261632398574 1104 72-73 0.3639835355131176 1104 74-75 0.2891544287591792 1104 76-77 0.21674045970528155 1104 78-79 -0.009755173870633627 1104 80-81 0.2938635203959912 1104 82-83 0.18269234903294418 1104 84-85 0.4175185000204209 1104 86-87 0.3851867239483475 1104 88-89 0.1869221678170021 1104 90-91 0.34312078664306256 1104 92-93 0.43056176543274205 1104 94-95 0.42414622233097177 1104 96-97 0.7772072300792168 1104 98-99 0.9170465650156281 1104 100-101 1.2271354945817272 1105 1 -0.11252591422744729 1105 2 -0.07403432010816147 1105 3 -0.06310579812407369 1105 4 0.008977582215301538 1105 5 0.016814799084585275 1105 6 0.04046468552955673 1105 7 -0.013300598903157379 1105 8 0.1103848584498266 1105 9 0.011543856114016648 1105 10-11 0.10294203106718669 1105 12-13 0.10642255370192544 1105 14-15 0.14882676484143076 1105 16-17 -0.007689886318175354 1105 18-19 0.09063792291529182 1105 20-21 0.21595053094237215 1105 22-23 0.206745897205586 1105 24-25 0.1624214312759804 1105 26-27 -0.06155294270472922 1105 28-29 -0.016695271950347035 1105 30-31 0.18249452461655125 1105 32-33 0.10399551425508946 1105 34-35 0.15861513602434485 1105 36-37 0.11500869980201855 1105 38-39 0.0723841512507164 1105 40-41 0.052906804095705695 1105 42-43 0.029650976613595503 1105 44-45 0.2695013286511241 1105 46-47 0.22394186778931413 1105 48-49 0.16395251353090146 1105 50-51 0.10512853260522093 1105 52-53 0.32267780944153657 1105 54-55 0.06750047981189056 1105 56-57 -0.06593723645308813 1105 58-59 0.05345494885292368 1105 60-61 0.1272698283395357 1105 62-63 -0.029660608901025398 1105 64-65 0.0683693372722658 1105 66-67 -0.0655195921785321 1105 68-69 0.036367737475167417 1105 70-71 0.18934895990881628 1105 72-73 0.1819452968431854 1105 74-75 0.011871730548875803 1105 76-77 -0.056148561024318866 1105 78-79 0.006891194519955235 1105 80-81 0.00881008014900786 1105 82-83 0.16353484601431134 1105 84-85 0.36575491219361567 1105 86-87 0.5728048335580418 1105 88-89 0.5689401164992489 1105 90-91 0.5153127866191909 1105 92-93 0.6068167359824237 1105 94-95 0.8424909571786259 1105 96-97 0.8551738699577953 1105 98-99 0.6680565886673868 1105 100-101 1.1443358094743807 1106 1 0.11735957898574867 1106 2 0.15477124980203172 1106 3 0.09119154840710308 1106 4 0.10559480352524986 1106 5 0.23575939727330564 1106 6 0.12125242598084185 1106 7 0.04728833589840775 1106 8 0.06721885253993065 1106 9 0.14771470247460172 1106 10-11 0.15957078984873618 1106 12-13 0.2495788577604401 1106 14-15 0.25697684375313656 1106 16-17 0.2123771587123855 1106 18-19 0.2544871037697689 1106 20-21 0.11233862395244643 1106 22-23 0.19267921032049884 1106 24-25 0.19343268841644345 1106 26-27 0.07486182166748279 1106 28-29 -0.08829194229490867 1106 30-31 0.09529371075841908 1106 32-33 0.04697306980754945 1106 34-35 0.24935749974451227 1106 36-37 -0.1012789877754301 1106 38-39 0.15910461123318953 1106 40-41 0.13176789154106672 1106 42-43 0.09881956536084857 1106 44-45 0.025367207663805402 1106 46-47 0.05729960123731104 1106 48-49 -0.1287588272100919 1106 50-51 0.14580909378624796 1106 52-53 0.008863071406487677 1106 54-55 0.0249164602824834 1106 56-57 -0.0942486853360478 1106 58-59 -0.0994469771516222 1106 60-61 -0.2068639285166114 1106 62-63 -0.08147423159726941 1106 64-65 0.1776013347263401 1106 66-67 0.1579535758425692 1106 68-69 -0.12319417280690104 1106 70-71 0.17565251462725584 1106 72-73 0.36168019936576457 1106 74-75 0.0643064961980393 1106 76-77 -0.11178643405925826 1106 78-79 -0.1251371608241918 1106 80-81 -0.024400678979706214 1106 82-83 -0.13702539225717203 1106 84-85 0.1841340096670443 1106 86-87 0.20660595907256507 1106 88-89 0.1010288028935129 1106 90-91 0.05943114521558002 1106 92-93 0.21777760834097393 1106 94-95 0.23748949067769587 1106 96-97 0.4629880004424578 1106 98-99 0.4138640919645198 1106 100-101 0.795835705721931 1107 1 0.04901035866900472 1107 2 0.07006527848306376 1107 3 -0.02918598760131985 1107 4 0.04102492076745534 1107 5 -0.03834238090342268 1107 6 -0.04336592304306208 1107 7 -0.03986893836271577 1107 8 -0.019938421721192867 1107 9 -0.018013902148695138 1107 10-11 -0.011100931975519757 1107 12-13 -0.05897497171294219 1107 14-15 -0.014606971951302228 1107 16-17 0.08643066704151181 1107 18-19 -0.026955816030593382 1107 20-21 -0.023884483898989117 1107 22-23 -0.1604921874132259 1107 24-25 -0.08487367338251062 1107 26-27 0.042276973783785365 1107 28-29 0.015351105373902385 1107 30-31 -0.08642532435531791 1107 32-33 -0.12128651001398794 1107 34-35 -0.10354824196122792 1107 36-37 -0.0836338541420929 1107 38-39 -0.0373726965369201 1107 40-41 -0.027975718473925326 1107 42-43 -0.16051546961146812 1107 44-45 -0.10623785561242016 1107 46-47 -0.14967492524677795 1107 48-49 -0.06378640927865575 1107 50-51 -0.16081823843797594 1107 52-53 0.10459057608616007 1107 54-55 0.13505077125618925 1107 56-57 -0.03988507638172223 1107 58-59 -0.10748344028289125 1107 60-61 0.036456860221541376 1107 62-63 0.1984926031381633 1107 64-65 0.04112140967964706 1107 66-67 0.09990196393410855 1107 68-69 0.054674323039829886 1107 70-71 0.041729344707572835 1107 72-73 0.16564863884038417 1107 74-75 0.18962776321524544 1107 76-77 -0.0350419185549633 1107 78-79 0.12792709061506358 1107 80-81 0.2553352412508083 1107 82-83 0.17182159183489176 1107 84-85 0.4838359936903629 1107 86-87 0.45271478572734125 1107 88-89 0.231926882313374 1107 90-91 0.4032499530731073 1107 92-93 0.3858352453870779 1107 94-95 0.5463837379725263 1107 96-97 0.767788640570366 1107 98-99 0.6471056651196108 1107 100-101 1.2599205820863055 1108 1 0.05315277541555474 1108 2 0.1225861638386121 1108 3 -0.025315924872039375 1108 4 0.04478150265620684 1108 5 0.08647263312427356 1108 6 0.0678892287430557 1108 7 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-1.3467319740516857 2316 68-69 -1.811002785079122 2316 70-71 -1.705208319169106 2316 72-73 -1.2802079054961304 2316 74-75 -1.2232534104450465 2316 76-77 -1.1836349118645977 2316 78-79 -1.742706558107983 2316 80-81 -1.3150553334489636 2316 82-83 -1.4810875983227092 2316 84-85 -1.3612346424077657 2316 86-87 -1.4570544805716814 2316 88-89 -1.5570693526053105 2316 90-91 -1.6251094605633014 2316 92-93 -1.8511735911234837 2316 94-95 -1.9974833084681443 2316 96-97 -1.8674656073931324 2316 98-99 -2.4350846137856728 2316 100-101 -3.4065233056050985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 16.0 4 21.0 5 91.0 6 185.0 7 449.0 8 1096.0 9 1800.0 10 3073.0 11 4801.0 12 6749.0 13 9292.0 14 11953.0 15 16022.0 16 20544.0 17 26135.0 18 22095.0 19 14297.0 20 12921.0 21 13145.0 22 13984.0 23 15645.0 24 18198.0 25 22213.0 26 26679.0 27 33096.0 28 40605.0 29 50259.0 30 60696.0 31 74450.0 32 91655.0 33 115398.0 34 149506.0 35 199633.0 36 272876.0 37 334281.0 38 188047.0 39 9538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6620848918385 19.560613180966637 15.073553646955254 21.70374828023961 2 16.090448505557347 21.230510476540807 38.5361388430701 24.142902174831743 3 19.195261529106215 25.790763957769226 30.18622407485959 24.82775043826497 4 13.008849557522122 17.27598394855032 36.55175529511813 33.16341119880943 5 13.43796059230077 38.41046789932439 33.00712391617134 15.144447592203502 6 31.304047410242102 36.56467086555582 16.944165404342396 15.187116319859683 7 28.596508012437216 34.06394004624093 19.85771612320285 17.481835818119006 8 28.051555980759517 33.103563740731886 19.162826543357518 19.68205373515108 9 27.8432060378963 16.043530255919634 20.050120917377555 36.06314278880651 10-11 25.574370831007997 26.80060591565016 26.046985038135478 21.57803821520636 12-13 26.992430769525143 24.42393643568493 27.033038115096314 21.550594679693617 14-15 23.525986675135336 25.29603575974849 26.332978109910204 24.844999455205972 16-17 22.119482314172583 28.816365037603976 26.769061096494724 22.29509155172872 18-19 22.01637592881972 26.255062665433552 30.414367871076102 21.314193534670622 20-21 23.070823035424805 25.14696112041245 30.543490393047918 21.23872545111483 22-23 22.618802114116363 25.258710312482723 30.562843086813984 21.559644486586926 24-25 22.521114034388372 25.61736958197135 30.583698742990777 21.2778176406495 26-27 22.05652277789338 25.97750048699957 30.43437704051491 21.53159969459214 28-29 21.75877129339803 25.990237021185898 30.67596722215933 21.57502446325675 30-31 21.835227909938382 25.745326808484855 30.598701089547646 21.820744192029117 32-33 21.677476590605625 25.881041579847086 30.443029698675804 21.998452130871485 34-35 22.49589870621555 25.786474407474998 30.058463866492225 21.65916301981723 36-37 22.70971836286055 25.395497462565668 29.965031880040055 21.929752294533728 38-39 22.221436766198412 25.698771650128236 30.076141693550078 22.003649890123267 40-41 22.22828730045258 25.78680216535695 30.15557191414046 21.829338620050017 42-43 22.383693194698807 25.9263895411246 29.497017965029887 22.192899299146703 44-45 22.334117224350777 26.06713576108341 29.421904071192788 22.17684294337302 46-47 22.77758333539292 26.457700011453937 28.52557484299996 22.23914181015319 48-49 22.94528848239001 26.61581710101667 27.95881009784037 22.48008431875295 50-51 22.849868067077043 26.779473368524304 27.86020983300898 22.51044873138967 52-53 23.285821156317144 26.779833080646682 27.63085337220411 22.30349239083206 54-55 22.648294747270945 26.76807754985325 27.80048814141644 22.783139561459365 56-57 22.871846722568996 27.112019426538613 27.43140056710353 22.584733283788854 58-59 23.140228714159406 26.422498608351592 27.556233494943545 22.88103918254546 60-61 22.888539488916148 26.815009549429092 28.013253570901686 22.283197390753067 62-63 22.84717851340892 26.83809145112315 27.956192428404965 22.358537607062967 64-65 22.58492978857495 26.779939167552907 27.90594813532841 22.729182908543734 66-67 22.628107546130835 27.107699209307174 27.491750771239104 22.772442473322887 68-69 22.84103197283124 26.68551053443253 27.824327883299127 22.6491296094371 70-71 22.697458899353343 26.935629649177216 27.67003720803413 22.69687424343531 72-73 22.9261269037516 26.905665020506795 27.513494250044978 22.654713825696625 74-75 22.752054784691094 27.449483802367542 27.176548971604415 22.62191244133695 76-77 22.78268338536 27.13036041643302 27.534074232741773 22.552881965465208 78-79 22.724228350847277 27.16313455375458 27.423624448043682 22.689012647354463 80-81 22.728772089771823 27.6470846219172 26.99483414646464 22.62930914184634 82-83 22.804365565617715 27.58525000199353 26.840413910057226 22.769970522331526 84-85 22.65057057286973 27.29961096941827 26.897421296672903 23.152397161039097 86-87 22.82652174109491 27.17367759535392 26.811682604280207 23.188118059270966 88-89 22.75472585042442 27.230438151168716 26.628038122666588 23.38679787574027 90-91 22.593633045940717 27.284226200368273 26.95312863533932 23.16901211835169 92-93 22.723457898781835 27.0021004153245 27.090040851895065 23.184400833998602 94-95 22.405471148861505 26.757347370100092 27.250255878560697 23.586925602477702 96-97 22.7405489692202 26.66277194781325 27.154681852701977 23.441997230264572 98-99 22.31558687034041 26.879418932042448 26.826155078388012 23.97883911922913 100-101 22.110073932237647 26.984495505558215 27.053534386468804 23.851896175735334 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 943.0 1 675.5 2 513.5 3 626.0 4 524.0 5 486.5 6 852.5 7 1655.0 8 2527.5 9 2955.0 10 2722.5 11 2379.5 12 2353.5 13 2483.0 14 2744.0 15 2954.0 16 3152.0 17 3407.5 18 3600.5 19 3762.0 20 4086.0 21 4634.5 22 5212.0 23 5904.0 24 6866.5 25 8135.0 26 10204.0 27 12620.0 28 14596.0 29 17315.0 30 21155.0 31 25060.0 32 30639.5 33 35105.5 34 37836.5 35 40184.5 36 43993.0 37 49188.0 38 51075.0 39 50358.0 40 50389.0 41 53526.0 42 56489.0 43 58736.5 44 60385.0 45 62263.0 46 62620.5 47 63200.0 48 70689.0 49 71626.0 50 65420.5 51 61644.0 52 59064.0 53 60555.0 54 62107.5 55 59084.5 56 54826.5 57 50871.5 58 48003.0 59 44365.5 60 38425.5 61 32756.0 62 28105.0 63 22025.0 64 16247.5 65 12931.0 66 9785.0 67 7689.0 68 5598.5 69 3551.5 70 2630.5 71 2144.5 72 1839.0 73 1720.0 74 1477.0 75 1157.0 76 851.0 77 532.0 78 329.5 79 248.0 80 175.5 81 120.0 82 88.0 83 59.5 84 35.0 85 22.5 86 12.5 87 3.0 88 4.5 89 5.0 90 1.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019878285365011027 2 0.013128172420207819 3 0.00892928326556645 4 0.0 5 0.0021791703207632413 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.035584256823195E-4 14-15 2.657524781418587E-4 16-17 0.0 18-19 0.001594514868851152 20-21 0.0 22-23 1.0630099125674347E-4 24-25 0.0 26-27 0.0016742406122937095 28-29 0.003029578250817189 30-31 0.001435063381966037 32-33 0.008185176326769248 34-35 0.0016742406122937095 36-37 0.0011693109038241783 38-39 0.001249036647266736 40-41 2.657524781418587E-4 42-43 0.005341624810651359 44-45 0.001249036647266736 46-47 7.97257434425576E-5 48-49 9.567089213106912E-4 50-51 7.97257434425576E-5 52-53 1.3287623907092935E-4 54-55 0.0014882138775944086 56-57 0.01658295463605198 58-59 0.031199340933854205 60-61 0.02378484679369635 62-63 0.016689255627308726 64-65 0.020276914082223818 66-67 0.0032953307289590476 68-69 0.0013021871428951074 70-71 7.97257434425576E-5 72-73 2.6575247814185868E-5 74-75 0.002577799037976029 76-77 0.0022854713120199844 78-79 0.009939142682505514 80-81 0.018469797230859175 82-83 0.019399930904355683 84-85 0.03300645778521885 86-87 0.011081878338515507 88-89 0.04725079061362247 90-91 0.05514363921443568 92-93 0.03356453798931675 94-95 0.03587658454915092 96-97 0.059847458077546566 98-99 0.06314278880650562 100-101 0.07175316909830184 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1881450.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.899700677410635 #Duplication Level Percentage of deduplicated Percentage of total 1 69.23577906912878 28.317226400947 2 15.230544920502334 12.45849456804805 3 5.96719876371091 7.321699299551732 4 2.9960600307132124 4.901518338708965 5 1.669968118655866 3.4150598096921745 6 1.039859902679512 2.5517975259620034 7 0.6763297789714444 1.9363179863405962 8 0.500651617198627 1.6381201029668335 9 0.3587561818999779 1.32057184102918 >10 1.9744513429935349 15.044167416773952 >50 0.20441425363730373 5.858330755302581 >100 0.13543802947607733 10.239267856652503 >500 0.007552882038016174 2.1670079614656688 >1k 0.0026044420820745423 1.4459147181623118 >5k 2.604442082074543E-4 0.8057240996154211 >10k+ 1.3022210410372715E-4 0.5787813187811199 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10867 0.5775864359935157 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9418 0.500571367828005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5710 0.3034893300380026 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2433 0.1293151558638284 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2301 0.12229929044088336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.3150495628371736E-5 0.0 0.0 0.0 0.0 3 5.3150495628371736E-5 0.0 0.0 0.0 0.0 4 5.3150495628371736E-5 5.3150495628371736E-5 0.0 0.0 0.0 5 5.3150495628371736E-5 5.3150495628371736E-5 0.0 0.0 0.0 6 1.0630099125674347E-4 5.3150495628371736E-5 0.0 0.0 0.0 7 1.594514868851152E-4 5.3150495628371736E-5 0.0 0.0 0.0 8 1.594514868851152E-4 5.3150495628371736E-5 0.0 0.0 0.0 9 1.594514868851152E-4 5.3150495628371736E-5 0.0 0.0 0.0 10-11 2.3917723032767282E-4 5.3150495628371736E-5 0.0 0.0 0.0 12-13 2.923277259560445E-4 5.3150495628371736E-5 0.0 0.0 0.0 14-15 3.189029737702304E-4 5.3150495628371736E-5 0.0 0.0 0.0 16-17 3.189029737702304E-4 5.3150495628371736E-5 0.0 0.0 0.0 18-19 4.252039650269739E-4 5.3150495628371736E-5 0.0 2.6575247814185868E-5 0.0 20-21 4.7835446065534564E-4 5.3150495628371736E-5 0.0 1.0630099125674347E-4 0.0 22-23 4.7835446065534564E-4 5.3150495628371736E-5 0.0 2.657524781418587E-4 0.0 24-25 5.84655451912089E-4 5.3150495628371736E-5 0.0 3.98628717212788E-4 0.0 26-27 5.84655451912089E-4 5.3150495628371736E-5 0.0 7.175316909830184E-4 0.0 28-29 6.112306997262749E-4 5.3150495628371736E-5 0.0 0.001833692099178825 0.0 30-31 6.378059475404608E-4 5.3150495628371736E-5 0.0 0.004703818863110899 0.0 32-33 6.378059475404608E-4 5.3150495628371736E-5 0.0 0.00861038029179622 0.0 34-35 7.97257434425576E-4 5.3150495628371736E-5 0.0 0.013075021924579447 0.0 36-37 9.567089213106913E-4 5.3150495628371736E-5 0.0 0.017778840787690344 0.0 38-39 0.0010630099125674348 5.3150495628371736E-5 0.0 0.021765127959818227 0.0 40-41 0.0010630099125674348 5.3150495628371736E-5 0.0 0.025379361662547506 0.0 42-43 0.0010630099125674348 5.3150495628371736E-5 0.0 0.03029578250817189 0.0 44-45 0.0010630099125674348 5.3150495628371736E-5 0.0 0.03523877860161046 0.0 46-47 0.0011161604081958065 5.3150495628371736E-5 0.0 0.043530255919636454 0.0 48-49 0.0011161604081958065 5.3150495628371736E-5 0.0 0.05256584017645964 0.0 50-51 0.0011161604081958065 5.3150495628371736E-5 0.0 0.061202795716070055 0.0 52-53 0.0011161604081958065 5.3150495628371736E-5 0.0 0.07611150973982833 0.0 54-55 0.0011161604081958065 5.3150495628371736E-5 0.0 0.10561003481357464 0.0 56-57 0.0011161604081958065 5.3150495628371736E-5 0.0 0.15482739376544685 0.0 58-59 0.0011161604081958065 5.3150495628371736E-5 0.0 0.20407132796513328 0.0 60-61 0.0011161604081958065 5.3150495628371736E-5 0.0 0.25281033245635015 0.0 62-63 0.0011161604081958065 5.3150495628371736E-5 0.0 0.30930930930930933 0.0 64-65 0.0011161604081958065 5.3150495628371736E-5 0.0 0.3765181110313853 0.0 66-67 0.0012756118950809216 5.3150495628371736E-5 0.0 0.4864067607430439 0.0 68-69 0.0012756118950809216 5.3150495628371736E-5 0.0 0.6228440830210742 0.0 70-71 0.001408488134151851 5.3150495628371736E-5 0.0 0.7387653139865529 0.0 72-73 0.0015147891254085944 5.3150495628371736E-5 0.0 0.8621276143400037 0.0 74-75 0.0015413643732227803 5.3150495628371736E-5 0.0 0.9948444019240479 0.0 76-77 0.0015413643732227803 5.3150495628371736E-5 0.0 1.1406362114326716 0.0 78-79 0.0015679396210369662 5.3150495628371736E-5 0.0 1.2600122246139946 0.0 80-81 0.001807116851364639 5.3150495628371736E-5 0.0 1.3914002498073295 0.0 82-83 0.001807116851364639 5.3150495628371736E-5 0.0 1.5384676712110341 0.0 84-85 0.001807116851364639 5.3150495628371736E-5 0.0 1.7718514975152142 0.0 86-87 0.001807116851364639 5.3150495628371736E-5 0.0 2.0861303781657767 0.0 88-89 0.001807116851364639 5.3150495628371736E-5 0.0 2.4368704988174015 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9800 0.0 33.358334 1 GTATCAA 14995 0.0 32.67038 1 GTAAGGT 790 0.0 28.854511 4 TAAGGTG 875 0.0 27.136982 5 GGTAAGG 805 0.0 26.549162 3 ATCAACG 19015 0.0 25.67621 3 TCAACGC 18990 0.0 25.632942 4 TATCAAC 19245 0.0 25.374748 2 CAACGCA 19405 0.0 25.109222 5 AACGCAG 19705 0.0 24.702845 6 AGGTAAG 755 0.0 24.538284 2 GTACATG 13145 0.0 24.508183 1 TACATGG 13730 0.0 23.215527 2 AGTACTT 11045 0.0 22.876 12-13 GAGTACT 10765 0.0 22.787174 12-13 TACCTGG 1205 0.0 22.470583 2 ACATGGG 13490 0.0 22.426508 3 ACGCAGA 22030 0.0 22.074203 7 CGCAGAG 22200 0.0 21.84099 8 GTACTTT 11495 0.0 21.236765 14-15 >>END_MODULE