##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469847 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.629194184489844 31.0 31.0 34.0 30.0 34.0 2 31.63418517091734 33.0 31.0 34.0 30.0 34.0 3 31.63912294853431 33.0 31.0 34.0 30.0 34.0 4 35.28437980874625 37.0 35.0 37.0 33.0 37.0 5 35.313874516597956 37.0 35.0 37.0 33.0 37.0 6 35.30843231945718 37.0 35.0 37.0 33.0 37.0 7 35.268974793922276 37.0 35.0 37.0 32.0 37.0 8 35.25684957017923 37.0 35.0 37.0 32.0 37.0 9 36.754845726374754 39.0 37.0 39.0 32.0 39.0 10-11 36.637709722526694 39.0 37.0 39.0 32.0 39.0 12-13 36.56041115512071 39.0 37.0 39.0 32.0 39.0 14-15 37.577982832709374 40.0 37.0 41.0 32.0 41.0 16-17 37.56293857362077 40.0 37.0 41.0 32.0 41.0 18-19 37.654618418336185 40.0 37.0 41.0 32.0 41.0 20-21 37.56239797210581 40.0 37.0 41.0 32.0 41.0 22-23 37.614323386123566 40.0 37.0 41.0 32.0 41.0 24-25 37.607592471591815 40.0 37.0 41.0 32.0 41.0 26-27 37.48068626595466 40.0 37.0 41.0 32.0 41.0 28-29 37.34314361909303 40.0 36.5 41.0 32.0 41.0 30-31 37.075553318420674 39.0 36.0 41.0 31.0 41.0 32-33 36.74482118647134 39.0 35.0 41.0 31.0 41.0 34-35 36.42699751195602 39.0 35.0 41.0 30.0 41.0 36-37 36.14075007396025 38.5 35.0 40.0 30.0 41.0 38-39 35.88449112157788 38.0 35.0 40.0 30.0 41.0 40-41 35.671300444612825 38.0 35.0 40.0 29.0 41.0 42-43 35.32618809953027 38.0 35.0 40.0 27.0 41.0 44-45 34.847724897679456 38.0 34.5 40.0 25.0 41.0 46-47 34.31829616875281 38.0 34.0 40.0 22.5 41.0 48-49 33.94910577273027 38.0 33.5 40.0 19.5 41.0 50-51 32.878803099732465 37.0 32.0 39.5 15.0 40.5 52-53 32.97721279480342 37.5 33.0 39.5 13.5 40.5 54-55 33.3965939976205 38.0 33.0 40.0 12.5 41.0 56-57 33.18998737887014 38.0 33.0 40.0 8.5 41.0 58-59 32.91846707545223 37.5 33.0 40.0 7.5 41.0 60-61 32.55177217264344 37.0 32.0 40.0 4.0 41.0 62-63 32.25016973610558 36.0 32.0 40.0 2.0 41.0 64-65 31.897098417144306 36.0 31.5 39.0 2.0 41.0 66-67 31.320294691676224 35.0 31.0 39.0 2.0 40.5 68-69 30.913372863932302 35.0 30.5 38.5 2.0 40.0 70-71 30.42140100926472 35.0 30.0 37.5 2.0 40.0 72-73 30.017497185253923 35.0 29.5 37.0 2.0 39.5 74-75 29.619298409907906 35.0 29.0 37.0 2.0 39.0 76-77 29.15252837625865 34.0 29.0 36.0 2.0 39.0 78-79 28.633549857719643 34.0 28.5 35.5 2.0 37.5 80-81 28.09351554867861 34.0 27.0 35.0 2.0 37.0 82-83 27.648952744191195 34.0 26.0 35.0 2.0 37.0 84-85 27.285036405468162 34.0 26.0 35.0 2.0 36.0 86-87 27.036053225837346 34.0 25.5 35.0 2.0 36.0 88-89 26.599577096373928 33.0 25.0 35.0 2.0 35.5 90-91 26.100211345395415 33.0 23.5 35.0 2.0 35.0 92-93 25.593627287180723 33.0 20.0 35.0 2.0 35.0 94-95 25.05431768213908 32.0 18.0 35.0 2.0 35.0 96-97 24.475324946205895 32.0 6.0 35.0 2.0 35.0 98-99 23.80457574487014 32.0 2.0 35.0 2.0 35.0 100-101 21.28480122252563 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26427829605647446 1101 2 0.2843775828064494 1101 3 0.3059457904510481 1101 4 0.3754119163547074 1101 5 0.12075149493940529 1101 6 0.3551408368462816 1101 7 0.2679199087329138 1101 8 0.3749873580853915 1101 9 0.31091858032988995 1101 10-11 0.4771736367035828 1101 12-13 0.4974334243585261 1101 14-15 0.5280316025543854 1101 16-17 0.4863568890766956 1101 18-19 0.3748292883937765 1101 20-21 0.366135749753127 1101 22-23 0.28481246551513806 1101 24-25 0.2689776800750323 1101 26-27 0.3250777818145494 1101 28-29 0.364347038467578 1101 30-31 0.32452070494933594 1101 32-33 0.2741477353688566 1101 34-35 0.2838339573524493 1101 36-37 0.1716456705051641 1101 38-39 0.20720826652123492 1101 40-41 0.3422900196380141 1101 42-43 0.311270457691478 1101 44-45 0.4583774951965509 1101 46-47 0.6588980867100744 1101 48-49 0.5525873667560646 1101 50-51 0.4216029299288948 1101 52-53 0.2396789991279462 1101 54-55 0.261488312225012 1101 56-57 0.37214192465800977 1101 58-59 0.45258793074029313 1101 60-61 0.45149925786879663 1101 62-63 0.36194900335311786 1101 64-65 0.33161772626588615 1101 66-67 0.38963363708147014 1101 68-69 0.531137195941632 1101 70-71 0.6436636616559106 1101 72-73 0.3572554787022675 1101 74-75 0.6295443594902324 1101 76-77 0.7789781707095678 1101 78-79 0.43290620739612606 1101 80-81 0.655018752067491 1101 82-83 0.42281639262478166 1101 84-85 0.5719613147412588 1101 86-87 0.6799973532057528 1101 88-89 0.7062695319795047 1101 90-91 0.8576985734576219 1101 92-93 0.8000369419947049 1101 94-95 0.8270560926307269 1101 96-97 0.831130914731748 1101 98-99 0.4292836496437289 1101 100-101 0.35844679685500225 1102 1 0.33693901940213067 1102 2 0.2080348996144501 1102 3 0.24125962679228152 1102 4 0.256800986442407 1102 5 0.11757145580045858 1102 6 0.21668501311648924 1102 7 0.35269196062824903 1102 8 0.23168446615236604 1102 9 0.3488688593760614 1102 10-11 0.45342119051962726 1102 12-13 0.3918887407316447 1102 14-15 0.3523194912258347 1102 16-17 0.30554493720309495 1102 18-19 0.26146950003398217 1102 20-21 0.18091613679379037 1102 22-23 -0.02313672633816566 1102 24-25 0.195612093353283 1102 26-27 -0.035411817373677934 1102 28-29 -0.09934615570507077 1102 30-31 0.29005125506893137 1102 32-33 0.30440703086788545 1102 34-35 -0.033531230731917105 1102 36-37 -0.0426373257983812 1102 38-39 -0.11400014835156469 1102 40-41 -0.13944876415555996 1102 42-43 -0.14299405633330764 1102 44-45 0.04509924630186646 1102 46-47 0.200650822812122 1102 48-49 0.5628549974004002 1102 50-51 0.5425304866441252 1102 52-53 0.524538932446788 1102 54-55 0.6417648216111118 1102 56-57 0.4975278781261565 1102 58-59 0.46336835402071586 1102 60-61 0.4941081583544076 1102 62-63 0.6153048053390506 1102 64-65 0.5426581698405819 1102 66-67 0.5611020722485378 1102 68-69 0.3367516411108724 1102 70-71 0.20475302890966418 1102 72-73 0.11097005433464702 1102 74-75 0.3147313566635326 1102 76-77 0.24388992688844624 1102 78-79 0.4296428168039661 1102 80-81 0.6808259927719931 1102 82-83 0.7040949314375666 1102 84-85 0.5626830296068093 1102 86-87 0.509242841355352 1102 88-89 0.726699697957617 1102 90-91 0.7211181574251491 1102 92-93 0.4225608602378017 1102 94-95 0.3979048278630266 1102 96-97 0.2731581670329746 1102 98-99 0.34231365616688336 1102 100-101 0.7123407593106101 1103 1 0.3566799760870225 1103 2 0.16730999975948535 1103 3 0.25904811958430685 1103 4 0.46539855247536366 1103 5 0.365453671342614 1103 6 0.3857154836580463 1103 7 0.4157625967817822 1103 8 0.49543409348058276 1103 9 0.19091094382741147 1103 10-11 0.40911808114802284 1103 12-13 0.06581830408360645 1103 14-15 0.6463592085108587 1103 16-17 0.6485800658617222 1103 18-19 0.6092030951902601 1103 20-21 0.5906202858356053 1103 22-23 0.38841116730971237 1103 24-25 0.6571697381124508 1103 26-27 0.6260609815090916 1103 28-29 0.5585910247218706 1103 30-31 0.29482425592299677 1103 32-33 0.3152559827915411 1103 34-35 0.6868265117625256 1103 36-37 0.8604629671832811 1103 38-39 0.5251779114238673 1103 40-41 0.5248597027231625 1103 42-43 0.6914107784245829 1103 44-45 0.4934099503321008 1103 46-47 0.5989276781571888 1103 48-49 0.708710887183706 1103 50-51 0.7612602418800165 1103 52-53 0.883717754378047 1103 54-55 0.9599323748443354 1103 56-57 0.8269529212215829 1103 58-59 0.9251585683882553 1103 60-61 1.0708673372884263 1103 62-63 1.2253892477211892 1103 64-65 1.026377176437709 1103 66-67 1.0037038929043014 1103 68-69 0.9731713692902417 1103 70-71 0.7538011187005864 1103 72-73 0.9109019086373564 1103 74-75 1.0238456195131427 1103 76-77 1.0343094040047163 1103 78-79 0.8752792505423628 1103 80-81 1.1235897715405727 1103 82-83 0.9058633571150452 1103 84-85 1.0548412307397328 1103 86-87 1.1896193463329041 1103 88-89 1.089669684709559 1103 90-91 0.9926546635683486 1103 92-93 1.047359393312 1103 94-95 1.2257769784650172 1103 96-97 1.1045367948386584 1103 98-99 1.4068609692313565 1103 100-101 1.5081126998714218 1104 1 0.4971990993567168 1104 2 0.5145965410547824 1104 3 0.4102648892315912 1104 4 0.6559176056425216 1104 5 0.48136960999448775 1104 6 0.680680299197256 1104 7 0.7021735013708366 1104 8 0.5323584991162633 1104 9 0.5594203256492634 1104 10-11 0.6060445057366337 1104 12-13 0.699351009017839 1104 14-15 0.7242508328005499 1104 16-17 0.7201554520215225 1104 18-19 0.7591089474898283 1104 20-21 0.4616715854664406 1104 22-23 0.5719662349516454 1104 24-25 0.5423887594530683 1104 26-27 0.454787877467119 1104 28-29 0.4481325681833326 1104 30-31 0.6320924443754237 1104 32-33 0.5121985309624932 1104 34-35 0.6078258654171904 1104 36-37 0.5528285210423647 1104 38-39 0.5545613876962676 1104 40-41 0.42523348942359007 1104 42-43 0.9062469978791583 1104 44-45 1.2813862444598882 1104 46-47 1.1568099367486084 1104 48-49 1.1104271373712251 1104 50-51 1.0990843434109152 1104 52-53 1.4146750324930224 1104 54-55 1.3742642766839595 1104 56-57 1.6973221232164164 1104 58-59 1.8301282770841922 1104 60-61 1.8246406060713483 1104 62-63 1.9154351087976025 1104 64-65 1.797213401500489 1104 66-67 1.86570916181061 1104 68-69 1.821684704894892 1104 70-71 1.7134515033531805 1104 72-73 1.5904029468558534 1104 74-75 1.6847225180649659 1104 76-77 1.7039617375076972 1104 78-79 1.8500409369849535 1104 80-81 1.6716191192645553 1104 82-83 1.5092709009887173 1104 84-85 1.4152299352589637 1104 86-87 1.3342745189884297 1104 88-89 1.3978523326504337 1104 90-91 1.2535664731810883 1104 92-93 1.0404711185212925 1104 94-95 1.1823645834961347 1104 96-97 1.4387309872645027 1104 98-99 1.5078128214103543 1104 100-101 0.8493564029114857 1105 1 0.2825266903178374 1105 2 0.15144043171694932 1105 3 0.24640890348304367 1105 4 0.2743659352033774 1105 5 0.3340821270301646 1105 6 0.44326587436354004 1105 7 0.30622953115922513 1105 8 0.5626606878170719 1105 9 0.5609756065619607 1105 10-11 0.4954678020196255 1105 12-13 0.24481321906408482 1105 14-15 0.30100116855474823 1105 16-17 0.34417323459562255 1105 18-19 0.18240027104579326 1105 20-21 0.2913068284493292 1105 22-23 -0.05084243573944036 1105 24-25 0.17237374226363755 1105 26-27 0.05271899677342873 1105 28-29 -0.2082128390760971 1105 30-31 0.018843593478067078 1105 32-33 -0.10431660822919753 1105 34-35 -0.018288769101722835 1105 36-37 -0.26058240440929836 1105 38-39 -0.07595623915138106 1105 40-41 0.13226240693617797 1105 42-43 0.2892755900523696 1105 44-45 0.47357948811329464 1105 46-47 0.5229317922146066 1105 48-49 0.2773540094116811 1105 50-51 0.4234675376484418 1105 52-53 0.5582597962946494 1105 54-55 0.5066545887121947 1105 56-57 0.3581945088467293 1105 58-59 0.2533660386699381 1105 60-61 0.4663635954640881 1105 62-63 0.7342012393816901 1105 64-65 0.4516474399341739 1105 66-67 0.5492620661588496 1105 68-69 0.5473859203548628 1105 70-71 0.3570888372366774 1105 72-73 0.023051744984755373 1105 74-75 0.3680145557805261 1105 76-77 0.2075880812684261 1105 78-79 0.5504747889436352 1105 80-81 0.8066597569297258 1105 82-83 0.38062298365665725 1105 84-85 0.35491122169845823 1105 86-87 0.4579372055378279 1105 88-89 0.7557097744910628 1105 90-91 0.885518370564288 1105 92-93 0.8975360415805156 1105 94-95 0.9942480009085344 1105 96-97 0.785753040909789 1105 98-99 1.374975167742054 1105 100-101 1.6113716422839026 1106 1 0.036736208514387414 1106 2 0.10356950199836845 1106 3 0.18939696890222635 1106 4 0.21482269076548022 1106 5 0.12029694948486025 1106 6 0.09764925435469962 1106 7 0.1632061040191033 1106 8 0.10842170151973818 1106 9 0.21280410221541501 1106 10-11 0.16194636397631257 1106 12-13 -0.0013544544293537797 1106 14-15 0.09702150154428324 1106 16-17 0.1593787745985722 1106 18-19 -0.027881149316662857 1106 20-21 -0.03684404822667631 1106 22-23 0.10014916585183897 1106 24-25 0.232294178391534 1106 26-27 0.13721582895259843 1106 28-29 -0.006814577694044033 1106 30-31 0.384874240302878 1106 32-33 0.31710228082340564 1106 34-35 0.24188951290800986 1106 36-37 0.052883044242541644 1106 38-39 -0.4243826425696824 1106 40-41 -0.5020870847390881 1106 42-43 -0.12744166352064212 1106 44-45 -0.2517571849381284 1106 46-47 -0.1844941691821802 1106 48-49 0.2009122825809726 1106 50-51 -0.058001447175485055 1106 52-53 -0.05987487292591709 1106 54-55 -0.08256892683222361 1106 56-57 0.12375808627417229 1106 58-59 -0.058060217407856385 1106 60-61 0.056061547431085046 1106 62-63 0.18090523230934252 1106 64-65 -0.0030792434310811245 1106 66-67 0.11990299735083099 1106 68-69 0.2683510006554357 1106 70-71 0.1503303283225783 1106 72-73 -0.330454959008172 1106 74-75 -0.26686978192390853 1106 76-77 -0.09268007844868364 1106 78-79 -0.07579749630757604 1106 80-81 0.06933693388567264 1106 82-83 -0.0865775467691563 1106 84-85 -0.14595956067961424 1106 86-87 -0.20040668719828503 1106 88-89 -0.3188651481551723 1106 90-91 -0.08236876660971859 1106 92-93 -0.062210532752771996 1106 94-95 0.2979315135061462 1106 96-97 0.3663497699506024 1106 98-99 0.39307152843160864 1106 100-101 0.2214939349021421 1107 1 0.44746747325762826 1107 2 0.3002125794797692 1107 3 0.2721079106550839 1107 4 0.589411694576043 1107 5 0.5641147683924999 1107 6 0.46765469801284354 1107 7 0.603071827118363 1107 8 0.7001037918844588 1107 9 0.9054406473070529 1107 10-11 0.5694640558652537 1107 12-13 0.4722837237597233 1107 14-15 0.3808592805317659 1107 16-17 0.5037082973712117 1107 18-19 0.61148375723387 1107 20-21 0.46542051355884695 1107 22-23 0.35857050499173937 1107 24-25 0.601185486684038 1107 26-27 0.7604348454650207 1107 28-29 0.5438713232313788 1107 30-31 0.7355819158608483 1107 32-33 0.25486130822315545 1107 34-35 0.40139051091200173 1107 36-37 0.14733595659964038 1107 38-39 0.27754259785855595 1107 40-41 0.5004514745060575 1107 42-43 0.450816365875113 1107 44-45 0.6622475328989239 1107 46-47 0.17077710644837651 1107 48-49 0.31240384491085393 1107 50-51 -0.0977056750833043 1107 52-53 0.3231481718935285 1107 54-55 0.10790104232037834 1107 56-57 0.17490306903592057 1107 58-59 -0.23380872040186773 1107 60-61 0.0847605131360396 1107 62-63 -0.007234851160085043 1107 64-65 0.12587920331179703 1107 66-67 0.012332683433211145 1107 68-69 0.33892439931266694 1107 70-71 0.3733043802187872 1107 72-73 0.433880007422605 1107 74-75 0.3873454240278278 1107 76-77 0.4920159839719318 1107 78-79 0.43863364141697403 1107 80-81 0.6775985923868504 1107 82-83 0.6232155942215911 1107 84-85 0.4893570787687622 1107 86-87 0.5701503804845274 1107 88-89 0.6299499489233966 1107 90-91 0.8467094406122015 1107 92-93 0.8022242340772081 1107 94-95 0.9597351789026298 1107 96-97 0.9828386104514202 1107 98-99 1.0185551067295542 1107 100-101 1.0159595491282367 1108 1 -0.10581907404089463 1108 2 0.0025867010155664616 1108 3 -0.27168411217885335 1108 4 0.095215811158603 1108 5 -0.08471533552742727 1108 6 -0.23232617562072733 1108 7 -0.07094623013323087 1108 8 -0.4465660134679794 1108 9 -0.23574626633495654 1108 10-11 -0.10043692840698526 1108 12-13 -0.16317263624753764 1108 14-15 0.12898710350988551 1108 16-17 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-1.0170958880769732 2316 84-85 -0.7935113948103947 2316 86-87 -1.0152511848059405 2316 88-89 -1.6090326122700027 2316 90-91 -1.3365753056846792 2316 92-93 -1.7700653971865812 2316 94-95 -1.633348390800073 2316 96-97 -1.7892881885820913 2316 98-99 -2.614356701233465 2316 100-101 -3.689530785991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1055.0 3 356.0 4 311.0 5 365.0 6 440.0 7 501.0 8 672.0 9 851.0 10 1192.0 11 1679.0 12 2563.0 13 3516.0 14 3765.0 15 5031.0 16 6674.0 17 9309.0 18 6725.0 19 4143.0 20 3868.0 21 3875.0 22 4296.0 23 4687.0 24 5108.0 25 5773.0 26 6680.0 27 7666.0 28 8856.0 29 10806.0 30 12939.0 31 15807.0 32 19566.0 33 25296.0 34 33617.0 35 46729.0 36 61238.0 37 76275.0 38 58235.0 39 9377.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.11610120967141 17.963503385504804 14.404945114592046 19.51545029023174 2 15.143000672643831 21.63997377540508 38.72065697718971 24.49636857476138 3 21.743517261050776 27.30136700540246 30.411932241488675 20.543183492058088 4 11.488265659092118 16.235218987813315 39.48443403757118 32.79208131552339 5 11.378960164697718 39.35159854292764 33.811785594910894 15.457655697463748 6 27.874971785819234 36.06070414077705 18.288751378768456 17.77557269463526 7 25.326349083370403 36.0196160649101 21.120686902828538 17.533347948890956 8 26.13622574430264 29.23350399693444 20.692092349941987 23.938177908820933 9 26.323330906179876 17.662651218047802 23.15057293596147 32.863444939810854 10-11 24.831263786334176 25.774696168357224 25.99591626425475 23.39812378105385 12-13 28.45831794245105 24.878833394187197 26.603960575212714 20.058888088149036 14-15 24.07916753594557 24.719950270150594 30.081875428430315 21.119006765473518 16-17 19.685139946671775 29.12391627149495 29.421745355069106 21.76919842676417 18-19 18.99773387930273 27.96524926795467 33.07950794632413 19.957508906418475 20-21 21.556074646951604 24.607238982675504 31.859423709180113 21.977262661192785 22-23 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30.897317200353307 20.576573125179582 48-49 21.197445098565048 26.498908143588697 30.304868445136872 21.99877831270938 50-51 19.115961539484815 27.391570400089392 32.235424326754746 21.25704373367104 52-53 20.522646563347696 28.686285423000836 28.848574885679046 21.942493127972423 54-55 19.145754847498033 26.876316965711 30.45878296405083 23.51914522274014 56-57 21.348939965052153 26.373597137767295 28.990428717678036 23.287034179502513 58-59 22.880640743078075 26.204186837991227 28.92623813532356 21.98893428360714 60-61 21.35948986672002 26.416977766804727 31.72795729591902 20.495575070556235 62-63 20.426182649402836 27.23544228900777 30.93176373935687 21.40661132223252 64-65 19.70503006845882 29.090156424093504 29.936925940390918 21.26788756705676 66-67 20.54364716149769 27.34296174886651 28.519338427808172 23.594052661827625 68-69 19.894947404504656 27.0986299940703 29.83544811830447 23.170974483120578 70-71 21.824846738767054 26.92979262088952 28.68236862334255 22.562992017000873 72-73 20.971497903318504 25.991187551884888 31.120607079759044 21.916707465037568 74-75 19.684168507331613 29.06522606913804 29.50166325145701 21.748942172073342 76-77 19.791565768759558 27.41992653390542 29.287234121818322 23.501273575516695 78-79 20.286250316766363 27.284391244258277 29.83481299604548 22.59454544292988 80-81 21.475179903235116 27.218771066809943 28.68728414008011 22.618764889874832 82-83 20.445711158642467 26.329153815278577 30.653182335511993 22.571952690566967 84-85 20.422946705394384 27.276220237113257 29.49919695684428 22.801636100648075 86-87 19.97217808156484 28.166020433251344 29.392356103526485 22.46944538165733 88-89 19.71911487658723 27.77058742137026 27.65095313614254 24.85934456589997 90-91 19.794418907669478 26.68817717104577 28.60379013525856 24.913613786026193 92-93 21.155479581822785 27.570659474234333 29.070170919610945 22.203690024331937 94-95 20.330910642844906 26.90580668781776 29.03301337073976 23.730269298597577 96-97 18.336341884999534 27.34166787292559 30.900521955331524 23.42146828674336 98-99 19.69656640936918 28.321639606068672 27.96209741815278 24.01969656640937 100-101 19.206546301837847 26.683804846256898 29.235150164956753 24.874498686948503 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 485.0 1 364.0 2 532.5 3 730.0 4 545.5 5 482.5 6 725.5 7 1360.5 8 2102.5 9 2312.5 10 1872.0 11 1432.5 12 1377.5 13 1461.0 14 1499.5 15 1490.5 16 1488.5 17 1517.0 18 1551.0 19 1629.0 20 1723.5 21 1894.5 22 2137.5 23 2411.0 24 2705.0 25 3015.5 26 3456.0 27 4004.0 28 4508.5 29 5085.0 30 5797.0 31 6591.0 32 7612.5 33 8416.5 34 9038.0 35 9490.5 36 10151.5 37 11096.0 38 11203.0 39 10915.5 40 10977.0 41 11320.5 42 11713.5 43 12245.0 44 12919.0 45 13502.0 46 14551.5 47 21469.5 48 36651.5 49 35703.0 50 20600.0 51 13916.5 52 11621.0 53 11051.0 54 10833.5 55 10138.0 56 9374.0 57 8370.5 58 7834.0 59 7412.0 60 6544.0 61 5813.5 62 5060.0 63 3790.5 64 2579.5 65 1908.5 66 1388.5 67 1044.0 68 791.0 69 539.0 70 414.5 71 343.0 72 295.0 73 292.5 74 239.5 75 192.0 76 152.5 77 90.5 78 53.5 79 36.5 80 28.0 81 25.0 82 19.0 83 12.0 84 8.0 85 6.5 86 4.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010641762105536483 2 0.01255727928453305 3 0.01298294976875451 4 0.015324137431972537 5 0.02937126341128069 6 0.04788792947491417 7 0.0059593867791004305 8 0.023837547116401722 9 0.034266473979827475 10-11 0.038097508337820614 12-13 0.030329022000778978 14-15 0.023199041390069532 16-17 0.024369635221678548 18-19 0.02160277707423906 20-21 0.047462258990692716 22-23 0.00904549778970601 24-25 0.0027668581474394856 26-27 9.577585894982836E-4 28-29 0.0 30-31 0.0011705938316090131 32-33 0.001809099557941202 34-35 0.0 36-37 4.256704842214593E-4 38-39 0.0 40-41 6.38505726332189E-4 42-43 0.002021934800051932 44-45 0.001277011452664378 46-47 4.256704842214593E-4 48-49 0.0010641762105536483 50-51 0.0030861110106055797 52-53 0.0015962643158304725 54-55 0.003618199115882404 56-57 8.513409684429186E-4 58-59 0.003724616736937769 60-61 0.0019155171789965672 62-63 3.192528631660945E-4 64-65 5.320881052768242E-4 66-67 0.01213160880031159 68-69 0.037778255474654514 70-71 0.047675094232803444 72-73 0.01213160880031159 74-75 0.02862634006389314 76-77 0.022666953284792707 78-79 0.05491149246456826 80-81 0.07630143429669659 82-83 0.04586599467486224 84-85 0.01521771981091717 86-87 0.015643390295138632 88-89 0.017452489853079834 90-91 0.03277662728505237 92-93 0.020006512758408587 94-95 0.011173850210813307 96-97 0.015962643158304726 98-99 0.04724942374858199 100-101 0.0702356298965408 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 469847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.64857286548758 #Duplication Level Percentage of deduplicated Percentage of total 1 84.37588967738114 46.95397844802085 2 9.216292994783752 10.257471045398127 3 2.6182346760071478 4.371030694401901 4 1.1072496538935692 2.4646745217992874 5 0.6071206483189661 1.6892698818060017 6 0.4146639539713874 1.3845274354360761 7 0.26615960596547295 1.0367981558493358 8 0.19513473935389622 0.8687175809218576 9 0.1467452122284184 0.7349545471819122 >10 0.9330613158966309 9.494759315348222 >50 0.06990176540725927 2.6579787267281203 >100 0.03610310960063754 3.4015544733892815 >500 0.0042248319745426905 1.47625113086395 >1k 0.00768151268098671 7.92819740093635 >5k 0.0015363025361973422 5.279836641918656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7352 1.5647646999980847 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6603 1.405351103657148 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5405 1.150374483608494 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5343 1.1371786985976284 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4875 1.037571805289807 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.8555976732851334 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3114 0.6627689439328122 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2607 0.5548614761826722 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2434 0.518040979297516 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2200 0.46823753264360524 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1671 0.35564768956702925 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1551 0.3301074605137417 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1448 0.3081854305763365 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1421 0.30243887903934685 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1383 0.29435113983913913 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1380 0.29371263411280696 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1235 0.26285152400675116 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1186 0.2524225971433254 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1165 0.2479530570590001 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1122 0.2388011416482387 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1117 0.23773696543768502 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.23220324914280605 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1039 0.22113581655304812 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.22028447558460523 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 827 0.17601474522557342 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 773 0.16452164215159404 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 757 0.16111627827782235 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 706 0.15026168093017514 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 602 0.12812681575065926 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 595 0.12663696905588415 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 586 0.12472145187688759 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 538 0.11450536025557256 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 512 0.10897164396069359 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 506 0.10769463250802921 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 505 0.10748179726591849 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.0641762105536483E-4 0.0 24-25 0.0 0.0 0.0 6.38505726332189E-4 0.0 26-27 0.0 0.0 0.0 0.0018090995579412021 0.0 28-29 0.0 0.0 0.0 0.004150287221159229 0.0 30-31 0.0 0.0 0.0 0.008619827305484551 0.0 32-33 0.0 0.0 0.0 0.014472796463529617 0.0 34-35 0.0 0.0 0.0 0.020645018484740776 0.0 36-37 0.0 0.0 0.0 0.026391570021730478 0.0 38-39 0.0 0.0 0.0 0.03937451979048499 0.0 40-41 0.0 0.0 0.0 0.06331848452794207 0.0 42-43 0.0 0.0 0.0 0.10450210387636827 0.0 44-45 0.0 0.0 0.0 0.1447279646352962 0.0 46-47 0.0 0.0 0.0 0.23294817249019362 0.0 48-49 0.0 0.0 0.0 0.32116838034509104 0.0 50-51 0.0 0.0 0.0 0.38789222874680485 0.0 52-53 0.0 0.0 0.0 0.4602562110644529 0.0 54-55 0.0 0.0 0.0 0.5379410744348693 0.0 56-57 0.0 0.0 0.0 0.6894797668177088 0.0 58-59 0.0 0.0 0.0 0.8402735358531608 0.0 60-61 0.0 0.0 0.0 1.003411748931035 0.0 62-63 0.0 0.0 0.0 1.1628253452719715 0.0 64-65 0.0 0.0 0.0 1.335009056139552 0.0 66-67 0.0 0.0 0.0 1.5997760973252995 0.0 68-69 0.0 0.0 0.0 1.9115797270175185 0.0 70-71 0.0 0.0 0.0 2.16868469948728 0.0 72-73 0.0 0.0 0.0 2.38609589930339 0.0 74-75 0.0 0.0 0.0 2.632984780151837 0.0 76-77 0.0 1.0641762105536483E-4 0.0 2.903179120011408 0.0 78-79 0.0 2.1283524211072966E-4 0.0 3.222857653661724 0.0 80-81 0.0 2.1283524211072966E-4 0.0 3.650762907925346 0.0 82-83 0.0 2.1283524211072966E-4 0.0 4.082924867031182 0.0 84-85 0.0 2.1283524211072966E-4 0.0 4.558185962664442 0.0 86-87 0.0 2.1283524211072966E-4 0.0 5.026317077686992 0.0 88-89 0.0 2.1283524211072966E-4 0.0 5.623745602291811 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAC 70 4.3086584E-6 40.7089 1 TACCTGG 475 0.0 32.99915 2 GTGTAGC 135 1.1956217E-8 31.66248 1 ATGGGAG 1000 0.0 28.974255 5 GCTTATG 115 3.8621474E-6 28.90922 1 GTACATG 4395 0.0 27.880274 1 TACATGG 4430 0.0 27.019623 2 ATACAGT 195 5.329639E-10 26.797033 6 GTACCTG 640 0.0 25.973127 1 ACATGGG 4415 0.0 25.712812 3 ATGGGTA 185 8.942152E-9 25.675013 5 CATGGGA 3275 0.0 25.671093 4 TGGGACG 130 9.998357E-6 25.578987 6 ACCTGGG 565 0.0 25.22059 3 GTCTAGA 95 0.0012264198 24.996695 1 ATGGGAT 1155 0.0 24.263445 5 GAGTACT 3745 0.0 24.100744 12-13 TGGGTAT 100 0.0016514191 23.751917 6 TGGGAGT 240 2.2919266E-10 23.751917 6 GTATTGT 120 1.710251E-4 23.74686 1 >>END_MODULE