##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 469847 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8744804159652 31.0 31.0 34.0 30.0 34.0 2 31.935093764565913 31.0 31.0 34.0 30.0 34.0 3 32.030758949189845 33.0 31.0 34.0 30.0 34.0 4 35.76434669158258 37.0 35.0 37.0 35.0 37.0 5 35.657903530298164 37.0 35.0 37.0 33.0 37.0 6 35.77479477361779 37.0 35.0 37.0 33.0 37.0 7 35.70517636592338 37.0 35.0 37.0 33.0 37.0 8 35.69047583575079 37.0 35.0 37.0 33.0 37.0 9 37.24566507820631 39.0 37.0 39.0 33.0 39.0 10-11 37.14063088622466 39.0 37.0 39.0 33.0 39.0 12-13 37.028549719376734 39.0 37.0 39.0 33.0 39.0 14-15 37.904096439904905 39.5 37.0 41.0 33.0 41.0 16-17 37.979129376158625 39.5 37.0 41.0 33.0 41.0 18-19 38.26086470702165 40.0 37.5 41.0 33.5 41.0 20-21 38.30664982430451 40.0 38.0 41.0 34.0 41.0 22-23 38.171121663009444 40.0 37.0 41.0 33.0 41.0 24-25 37.984166122163174 40.0 37.0 41.0 33.0 41.0 26-27 37.68729394888123 39.5 36.5 41.0 32.5 41.0 28-29 37.6920295330182 39.5 37.0 41.0 33.0 41.0 30-31 37.49463335937018 39.0 36.0 41.0 33.0 41.0 32-33 37.14100547625078 39.0 35.0 41.0 32.0 41.0 34-35 36.79085425681126 39.0 35.0 41.0 31.0 41.0 36-37 36.5099319565731 38.5 35.0 40.5 30.5 41.0 38-39 36.25363788637578 38.0 35.0 40.0 30.0 41.0 40-41 36.05514135452604 38.0 35.0 40.0 30.0 41.0 42-43 35.65637962996465 38.0 35.0 40.0 29.5 41.0 44-45 35.149647651256686 38.0 35.0 40.0 26.5 41.0 46-47 34.48530585488467 38.0 34.0 40.0 23.5 41.0 48-49 33.78823957586193 38.0 33.0 40.0 19.5 41.0 50-51 33.392258543738706 38.0 33.0 40.0 17.0 41.0 52-53 33.16892094660602 38.0 33.0 40.0 14.0 41.0 54-55 32.846477683160686 37.0 32.5 40.0 9.5 41.0 56-57 32.42184902744936 37.0 31.5 40.0 7.5 41.0 58-59 32.02771540522766 36.0 31.0 39.0 6.5 40.5 60-61 31.512269951707683 35.5 31.0 39.0 2.0 40.0 62-63 31.27459151596158 35.0 30.5 39.0 2.0 40.0 64-65 31.002350765249112 35.0 30.5 38.5 2.0 40.0 66-67 30.518165487914153 35.0 29.5 38.0 2.0 40.0 68-69 29.963200786639057 34.5 29.0 37.0 2.0 39.5 70-71 29.35961493847997 34.0 28.5 36.5 2.0 39.0 72-73 28.759266314353397 34.0 27.0 36.0 2.0 39.0 74-75 28.282816533892948 33.5 26.0 35.5 2.0 38.0 76-77 26.937809542255245 31.5 25.5 34.0 2.0 36.0 78-79 27.696357537666515 33.0 26.0 35.0 2.0 37.0 80-81 27.440979723186487 33.0 26.0 35.0 2.0 36.5 82-83 27.018044171826148 33.0 25.5 35.0 2.0 36.0 84-85 26.577751906471683 33.0 25.0 35.0 2.0 35.5 86-87 26.266423963545577 32.0 24.5 35.0 2.0 35.0 88-89 25.741871290015688 32.0 23.0 34.0 2.0 35.0 90-91 25.080815669781863 31.5 19.5 34.0 2.0 35.0 92-93 24.48985095147995 31.0 17.5 34.0 2.0 35.0 94-95 23.71621719410787 31.0 4.5 34.0 2.0 35.0 96-97 22.879751280736066 31.0 2.0 34.0 2.0 35.0 98-99 21.792783608280992 30.0 2.0 34.0 2.0 35.0 100-101 18.361162250690118 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22839103766290592 1101 2 0.4441405914818368 1101 3 0.381879899575992 1101 4 0.2609299453108136 1101 5 0.18378490754567878 1101 6 0.14206654291199072 1101 7 0.16132467900997938 1101 8 0.1571462506718717 1101 9 0.21445479871244544 1101 10-11 0.23238310012201424 1101 12-13 0.30916569544864814 1101 14-15 0.4798423986993825 1101 16-17 0.3738552704962359 1101 18-19 0.3258785552021237 1101 20-21 0.2210374667717403 1101 22-23 0.37339563986879654 1101 24-25 0.36944850114105776 1101 26-27 0.3253575493601417 1101 28-29 0.4590723047097427 1101 30-31 0.5514481670419116 1101 32-33 0.5126123863000416 1101 34-35 0.4765673909511179 1101 36-37 0.5331463474165048 1101 38-39 0.5472068491773285 1101 40-41 0.47510426998387345 1101 42-43 0.6008029981691791 1101 44-45 0.9498699406818787 1101 46-47 1.0313160457969204 1101 48-49 1.1462203844413992 1101 50-51 1.0394310877288788 1101 52-53 0.9851772442407807 1101 54-55 0.9807092368271668 1101 56-57 1.030659664963487 1101 58-59 0.8898058678409058 1101 60-61 0.777340536202086 1101 62-63 0.691681139019547 1101 64-65 0.8242994523329941 1101 66-67 0.6430056500471899 1101 68-69 0.7994184382609753 1101 70-71 0.6460566250837481 1101 72-73 0.8404925578484992 1101 74-75 0.764795685282305 1101 76-77 0.6234869999235393 1101 78-79 0.966175088536982 1101 80-81 1.1107480602584303 1101 82-83 1.0617923248536378 1101 84-85 0.953550712496682 1101 86-87 0.8144032515302229 1101 88-89 0.5780707262263469 1101 90-91 0.5469542890924757 1101 92-93 0.6553480745980131 1101 94-95 0.7847065274540377 1101 96-97 0.8352640712216655 1101 98-99 0.04015253633911797 1101 100-101 0.20383628266889886 1102 1 0.17776046579945515 1102 2 0.3906235225538133 1102 3 0.19664397872363537 1102 4 0.1661158552912383 1102 5 0.05393421536621901 1102 6 0.13918185783552417 1102 7 0.20868280034070352 1102 8 0.10721510622018116 1102 9 0.1223713747797035 1102 10-11 0.3548209489297278 1102 12-13 0.21247710372580997 1102 14-15 0.4166420652935727 1102 16-17 0.3204396729025589 1102 18-19 0.22213045778964613 1102 20-21 0.24637540255488233 1102 22-23 0.11935309438788977 1102 24-25 0.23174646446641844 1102 26-27 0.2924264773879699 1102 28-29 0.2779831186547952 1102 30-31 0.3386292571339595 1102 32-33 0.253898535462902 1102 34-35 0.22969488243477088 1102 36-37 0.1861352218094936 1102 38-39 0.38066158571425746 1102 40-41 0.3557685448262262 1102 42-43 0.551188045643265 1102 44-45 0.38233804969108576 1102 46-47 0.16951819119084632 1102 48-49 0.4469922913776898 1102 50-51 0.2666960728705803 1102 52-53 0.33527962156917823 1102 54-55 0.5465831586942471 1102 56-57 0.6672645428354897 1102 58-59 0.6921496744655329 1102 60-61 0.5856059199811661 1102 62-63 0.6025739055629984 1102 64-65 0.6333865727214842 1102 66-67 0.8394641192796328 1102 68-69 0.6942488361735641 1102 70-71 0.4133801374415036 1102 72-73 0.3782199623567237 1102 74-75 0.6526517409465811 1102 76-77 0.6557530722581042 1102 78-79 0.8110870259146736 1102 80-81 0.9704014269323444 1102 82-83 0.9239679789813451 1102 84-85 0.7335898514399339 1102 86-87 0.8833684251186824 1102 88-89 0.8565506493980521 1102 90-91 1.014004191245121 1102 92-93 0.8440421747647164 1102 94-95 0.716316660091568 1102 96-97 0.7583297739106598 1102 98-99 0.7978652637436277 1102 100-101 0.925565644124287 1103 1 0.3177953904693176 1103 2 0.39216464646466065 1103 3 0.366645077124673 1103 4 0.21241906329477445 1103 5 0.26879827142501966 1103 6 0.30659498888373804 1103 7 0.1310841291818008 1103 8 0.26604277606324445 1103 9 0.31360523781133764 1103 10-11 0.3035648488810807 1103 12-13 0.34645569163038914 1103 14-15 0.48286836280782097 1103 16-17 0.39864526667798117 1103 18-19 0.30759390457211566 1103 20-21 0.17201589365222958 1103 22-23 0.247517442083371 1103 24-25 0.4447157787279181 1103 26-27 0.40737358601535334 1103 28-29 0.4137639809220346 1103 30-31 0.5849199119827304 1103 32-33 0.6786776783962623 1103 34-35 0.8313020224898722 1103 36-37 0.7071679205360155 1103 38-39 0.6355898197767971 1103 40-41 0.5165991153446896 1103 42-43 0.6617947889290079 1103 44-45 0.913152682186265 1103 46-47 0.9595997418641176 1103 48-49 0.9262891129637367 1103 50-51 0.9172565936471742 1103 52-53 0.963522986890645 1103 54-55 0.8752443647385917 1103 56-57 0.9089194969604293 1103 58-59 0.7833959785701907 1103 60-61 0.7524121284128533 1103 62-63 0.8037897682673538 1103 64-65 0.8626060579076444 1103 66-67 0.8756373797150019 1103 68-69 0.963536422224319 1103 70-71 0.45384108480119423 1103 72-73 1.1254963760997398 1103 74-75 0.9715539899787551 1103 76-77 1.0246802034363292 1103 78-79 0.9441246876206009 1103 80-81 1.0879196906517095 1103 82-83 0.9590097742618084 1103 84-85 1.2462769438827088 1103 86-87 1.1158875967001336 1103 88-89 1.0667352928548297 1103 90-91 1.142587164235664 1103 92-93 1.1738236377900755 1103 94-95 1.1261440990462503 1103 96-97 1.4480981682052487 1103 98-99 1.254695300753017 1103 100-101 0.8695102040129221 1104 1 0.18827815856593588 1104 2 0.2744989486149798 1104 3 0.28364788543210295 1104 4 0.2644183174038375 1104 5 0.13716674715762878 1104 6 0.19954421642393783 1104 7 0.2474935896586956 1104 8 0.15647532270596543 1104 9 0.31326594155663656 1104 10-11 0.12979118752397767 1104 12-13 0.24525357341229181 1104 14-15 0.21615977482704096 1104 16-17 0.27785930513062596 1104 18-19 0.3069798063921141 1104 20-21 0.3559081544729068 1104 22-23 0.22476356225874383 1104 24-25 0.36206489354324844 1104 26-27 0.5237935618720471 1104 28-29 0.6460634421139773 1104 30-31 0.5702460499921855 1104 32-33 0.613588178493707 1104 34-35 0.5801554955796178 1104 36-37 0.670154484684744 1104 38-39 0.7768625452650895 1104 40-41 0.6274536511888158 1104 42-43 0.6262682277806775 1104 44-45 0.6665768403600154 1104 46-47 0.7760993541971217 1104 48-49 0.9729577705423083 1104 50-51 0.9441423167057152 1104 52-53 0.9790703264294507 1104 54-55 1.0665149170483943 1104 56-57 1.233124078581504 1104 58-59 1.4635994441589588 1104 60-61 1.023666865591455 1104 62-63 0.9883451990403991 1104 64-65 1.2761479042120492 1104 66-67 1.463770983924519 1104 68-69 1.2275804129651782 1104 70-71 1.1539031956445136 1104 72-73 1.357398355935448 1104 74-75 1.2322919453122267 1104 76-77 1.1293678195436492 1104 78-79 1.281046569565131 1104 80-81 1.297212201878633 1104 82-83 1.2674595128494666 1104 84-85 1.41738180184797 1104 86-87 1.2804981561043185 1104 88-89 1.4726177365169306 1104 90-91 1.2304151214058194 1104 92-93 0.9993068895941626 1104 94-95 1.3091285320780024 1104 96-97 1.615286397709724 1104 98-99 1.4828692279389166 1104 100-101 1.2983736597032127 1105 1 0.046876540994436056 1105 2 0.14945753727690558 1105 3 0.12448749907176193 1105 4 0.13264793558633414 1105 5 0.1473871645839111 1105 6 0.20292853943038835 1105 7 0.16683521385601097 1105 8 0.1376492820663131 1105 9 0.21245437851915483 1105 10-11 0.19876313493803366 1105 12-13 0.1572155483809965 1105 14-15 0.32916858874681054 1105 16-17 0.3042187376911514 1105 18-19 0.4559951588397979 1105 20-21 0.3421199146977827 1105 22-23 0.33367686923429574 1105 24-25 0.3514477207820903 1105 26-27 0.2246192097239046 1105 28-29 0.17846347464790568 1105 30-31 0.183638724698433 1105 32-33 0.2649939818339888 1105 34-35 0.17664242546699427 1105 36-37 0.03139129009014141 1105 38-39 0.17794548895162166 1105 40-41 0.4271527122673149 1105 42-43 0.5857803377453834 1105 44-45 0.5947968570634146 1105 46-47 0.6044401828999852 1105 48-49 0.016380658167314266 1105 50-51 0.11866903718563293 1105 52-53 0.35955670879447155 1105 54-55 0.5058480506815428 1105 56-57 0.30727665878064414 1105 58-59 0.5467067922661357 1105 60-61 0.491051593288347 1105 62-63 0.6094200489180999 1105 64-65 0.6711585074983724 1105 66-67 0.35750006747920793 1105 68-69 0.5532947213511967 1105 70-71 0.7317813084380909 1105 72-73 0.8829275779577515 1105 74-75 0.8902233820228069 1105 76-77 0.7030671067726892 1105 78-79 0.6383793024753928 1105 80-81 0.911199017698852 1105 82-83 0.5754973791786284 1105 84-85 0.7111682165485469 1105 86-87 1.0707564239895504 1105 88-89 1.0568697237652174 1105 90-91 0.9381820038412627 1105 92-93 1.0370869244689551 1105 94-95 1.1319197455343577 1105 96-97 1.2764077473126711 1105 98-99 1.2209789664969897 1105 100-101 1.0824544470244994 1106 1 -0.13777057849871355 1106 2 0.06934261168386158 1106 3 -0.14216050446441386 1106 4 0.018202672583541357 1106 5 0.16745494121570914 1106 6 -0.03180551096006212 1106 7 0.035870133555434336 1106 8 0.08637183989745978 1106 9 0.06699688625452893 1106 10-11 0.08246727520618435 1106 12-13 0.10003269881564592 1106 14-15 0.28911849297547576 1106 16-17 0.10404045568142095 1106 18-19 0.09499471681828453 1106 20-21 -0.2176914894572164 1106 22-23 -0.17941580794265377 1106 24-25 -0.022453855761305874 1106 26-27 -0.20666265266006434 1106 28-29 -0.11502702188958835 1106 30-31 -0.08047944238570182 1106 32-33 -0.20349872481106956 1106 34-35 -0.24477941039567952 1106 36-37 -0.3549428781727215 1106 38-39 -0.283887426916948 1106 40-41 -0.40484522496562647 1106 42-43 -0.37087208600591026 1106 44-45 -0.07402736571543045 1106 46-47 -0.24910482962395264 1106 48-49 -0.2069109286899149 1106 50-51 -0.08290897961118304 1106 52-53 -0.37245743589390656 1106 54-55 -0.5674641638462248 1106 56-57 -0.431772994969176 1106 58-59 -0.8689230883880512 1106 60-61 -0.8225332011716517 1106 62-63 -0.9053979855595777 1106 64-65 -1.1054143523808087 1106 66-67 -1.0192923297507868 1106 68-69 -0.6440664102238749 1106 70-71 -0.6515780550509369 1106 72-73 -0.5681774758215354 1106 74-75 -0.5128810823944612 1106 76-77 -0.5084490270124853 1106 78-79 -0.3874612750993798 1106 80-81 -0.6114825794722094 1106 82-83 -0.45942821386690014 1106 84-85 -0.35190383295786276 1106 86-87 -0.43494860032163274 1106 88-89 -0.6011632805076594 1106 90-91 -0.25827298363479656 1106 92-93 -0.556562699812762 1106 94-95 -0.7310005432530318 1106 96-97 -0.5651820567244599 1106 98-99 -0.33101749733091523 1106 100-101 -0.4452882964556828 1107 1 0.22942230845465517 1107 2 0.4177766526927549 1107 3 0.3051666593896982 1107 4 0.20114950618905425 1107 5 0.2199680967228801 1107 6 0.14736705300292385 1107 7 0.21761210415968435 1107 8 0.2867093467020325 1107 9 0.2195390746051018 1107 10-11 0.41006662196721777 1107 12-13 0.4970205413124731 1107 14-15 0.3318439511500344 1107 16-17 0.22685482693891146 1107 18-19 0.41015167562794375 1107 20-21 0.2389987663947153 1107 22-23 0.4337360701194015 1107 24-25 0.4820843405733015 1107 26-27 0.530014901322879 1107 28-29 0.3806652299703259 1107 30-31 0.5316072932339679 1107 32-33 0.461531215308689 1107 34-35 0.37045738873332823 1107 36-37 0.4146361455979246 1107 38-39 0.245305097125879 1107 40-41 0.16071637909897163 1107 42-43 0.26810450626410187 1107 44-45 0.10233445609549108 1107 46-47 -0.14213150354883197 1107 48-49 0.0836788009417333 1107 50-51 -0.045560041124524275 1107 52-53 -0.043562498495973045 1107 54-55 0.11974505408154812 1107 56-57 -0.06436417624299651 1107 58-59 -0.09063602614889632 1107 60-61 -0.11796052833550519 1107 62-63 -0.13245115195406143 1107 64-65 0.05840346652682982 1107 66-67 -0.03982036459020222 1107 68-69 0.18499728057634357 1107 70-71 0.22150849911346526 1107 72-73 0.4059560752581248 1107 74-75 0.17430999000618996 1107 76-77 0.15453046854185715 1107 78-79 0.40080582705993706 1107 80-81 0.04090718645048952 1107 82-83 0.05229742576293006 1107 84-85 -0.0697027805172894 1107 86-87 0.2078857310156934 1107 88-89 0.1871165235206469 1107 90-91 0.5180870236234156 1107 92-93 0.3803453023647059 1107 94-95 0.572015243355569 1107 96-97 1.7103777327874283 1107 98-99 1.4559903052457521 1107 100-101 1.2244395206374072 1108 1 -0.15202119274932713 1108 2 0.06968659202783911 1108 3 -0.10004748235138905 1108 4 0.05048768486854982 1108 5 -0.10084972708895634 1108 6 -0.08207578123032988 1108 7 -0.21607089838559546 1108 8 -0.006453712928092159 1108 9 -0.3668606076029661 1108 10-11 -0.1919798992409909 1108 12-13 -0.19701889823594598 1108 14-15 -0.29559895174196527 1108 16-17 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-1.2304462723805436 2316 94-95 -1.7283051684523905 2316 96-97 -1.3326940184469471 2316 98-99 -0.9941554558636092 2316 100-101 -0.8002085516390949 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 8.0 5 24.0 6 68.0 7 159.0 8 284.0 9 574.0 10 984.0 11 1586.0 12 2430.0 13 3178.0 14 4008.0 15 5375.0 16 7331.0 17 9808.0 18 8194.0 19 4741.0 20 4189.0 21 4364.0 22 4471.0 23 4745.0 24 5448.0 25 6278.0 26 7292.0 27 8889.0 28 10594.0 29 12735.0 30 15418.0 31 18953.0 32 23229.0 33 29188.0 34 38513.0 35 50830.0 36 67250.0 37 72822.0 38 34573.0 39 1307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.931722633175454 18.820073439412486 14.476717577563727 17.77148634984833 2 15.711591593125673 24.672950111322546 37.57870269856068 22.0367555969911 3 23.60094164523984 27.996705093057706 29.705672007764743 18.69668125393771 4 12.120567164560303 17.68771044129353 38.74290725046079 31.448815143685376 5 11.836812014967625 41.54601608296049 31.713401473774727 14.903770428297156 6 27.557694313255165 38.583836866043626 16.68755999293387 17.170908827767338 7 27.143516932107687 36.90775933442163 19.30777465855906 16.64094907491162 8 27.28536218866929 29.942873926757734 18.97888208276044 23.79288180181254 9 26.87279050414284 19.413979444372316 21.262027851619784 32.451202199865065 10-11 26.043158730395216 26.868427381679567 25.144461920582657 21.94395196734256 12-13 30.362649969032475 25.68953297562824 25.342930783850914 18.60488627148838 14-15 23.910119294661005 26.453404846279092 29.610722685140843 20.025753173919057 16-17 19.506173102280552 29.793506243341874 29.089833244824355 21.610487409553222 18-19 19.582855330678512 28.859051117728065 32.3926170139857 19.165476537607724 20-21 22.53298697760642 24.320812524143626 32.25769376889588 20.888506729354074 22-23 20.88652452080043 25.842722934748835 32.64229130395917 20.628461240491568 24-25 22.9051646709773 25.589342039732166 32.84863551035871 18.65685777893182 26-27 21.54131353677588 24.525163564231683 35.43544054860529 18.49808235038715 28-29 19.333122626219414 28.654784062454443 33.01014267425638 19.00195063706977 30-31 20.60658302206047 25.768604539795035 32.57137992316615 21.053432514978343 32-33 20.739137329021148 26.317839480750017 33.048231121157265 19.89479206907157 34-35 22.249619022484058 25.179208418113248 33.318080043588935 19.253092515813762 36-37 21.99528990095596 24.571158571560836 34.405432470311794 19.02811905717141 38-39 20.281828640268866 26.5709855488592 34.139556366260166 19.007629444611766 40-41 20.212771617964588 27.432576383989236 32.17050675060897 20.184145247437197 42-43 19.867633537094108 27.664250200071518 30.6440387202234 21.824077542610976 44-45 21.73404056055988 25.395473972789347 31.0639697243176 21.80651574233317 46-47 23.439651631275712 27.561844600476327 30.158753807090395 18.83974996115757 48-49 22.750317666064323 27.641204427956318 29.721305229730564 19.887172676248795 50-51 19.921462625574662 28.424144389579432 31.859675634258473 19.794717350587433 52-53 21.855325832939585 30.008385797778832 27.8920360461602 20.24425232312139 54-55 20.177355984726912 28.004920761270714 29.061330733900043 22.75639252010233 56-57 22.673650062475918 27.08826027446832 28.75094991070307 21.48713975235269 58-59 24.714338468873347 26.861495794760025 27.90272236476772 20.521443371598906 60-61 21.61410581275189 27.660138286856363 31.318237497086653 19.40751840330509 62-63 20.581020782363566 27.874588561559065 30.678063320815035 20.866327335262337 64-65 20.446191208992364 30.361243963126384 28.867164303000504 20.325400524880752 66-67 21.858917328951684 27.262162702889082 28.127993052924626 22.75092691523461 68-69 21.241870755792235 28.039307709253208 28.6345210995857 22.08430043536885 70-71 23.29337442743542 27.946898360209104 28.222644609804686 20.537082602550786 72-73 22.275397993510357 26.64574400495503 30.474931650155217 20.603926351379396 74-75 20.492525590513473 30.80535884189756 28.008654691434494 20.693460876154475 76-77 21.664077132640898 27.939085646177418 27.815426528179803 22.581410693001875 78-79 21.891981635090005 28.345348854534503 28.2176373802971 21.545032130078393 80-81 22.777624648941796 27.866066307032654 28.356244126858787 21.000064917166757 82-83 21.946147518041464 27.321711164157374 29.47945013042215 21.25269118737901 84-85 20.390034693399738 28.72847625736969 29.32667134921142 21.554817700019154 86-87 20.68365535648154 29.094993636158538 27.454335712856665 22.76701529450326 88-89 20.246942222288443 28.793075741789785 26.57534217418558 24.384639861736193 90-91 21.729829371296233 26.775573019549075 27.626055786390573 23.868541822764115 92-93 22.658141497516628 28.512880873602803 27.646280685660287 21.18269694322029 94-95 21.36800434185742 28.33200310740776 27.895050495376132 22.40494205535868 96-97 18.97694246950866 28.47334182546853 29.99788228708738 22.551833417935434 98-99 20.926889018720182 29.714658485166773 26.32340123935676 23.03505125675628 100-101 19.820552305565517 28.269663208327255 27.07369998329185 24.83608450281538 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 887.0 1 635.5 2 762.0 3 984.5 4 658.5 5 524.5 6 781.0 7 1414.5 8 2120.5 9 2354.0 10 1972.5 11 1567.5 12 1453.5 13 1456.0 14 1499.0 15 1509.0 16 1564.5 17 1646.0 18 1681.0 19 1752.0 20 1889.0 21 2025.0 22 2168.5 23 2432.0 24 2726.5 25 3036.0 26 3477.5 27 4058.0 28 4600.0 29 5137.5 30 5779.0 31 6576.0 32 7693.0 33 8443.0 34 8909.0 35 9373.0 36 9959.0 37 10686.5 38 10822.5 39 10574.5 40 10579.0 41 11061.0 42 11644.5 43 12165.0 44 12797.0 45 13315.5 46 13889.5 47 21356.0 48 37866.5 49 36352.0 50 20426.5 51 13585.5 52 11223.0 53 10646.5 54 10372.5 55 9802.0 56 9112.5 57 8545.0 58 8268.0 59 7685.0 60 6671.5 61 5775.0 62 5061.5 63 3880.5 64 2565.5 65 1916.0 66 1428.0 67 1097.5 68 839.5 69 552.0 70 411.5 71 341.5 72 301.5 73 287.0 74 239.5 75 196.0 76 147.0 77 93.0 78 56.0 79 43.0 80 32.0 81 23.0 82 20.5 83 15.5 84 7.5 85 4.0 86 2.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015324137431972537 2 0.008726244926539917 3 0.00659789250543262 4 2.1283524211072966E-4 5 0.007023562989654079 6 0.0 7 0.0 8 0.0019155171789965672 9 0.0 10-11 0.0 12-13 0.0 14-15 4.256704842214593E-4 16-17 0.0056401339159343365 18-19 0.0021283524211072966 20-21 5.320881052768242E-4 22-23 2.1283524211072966E-4 24-25 2.1283524211072966E-4 26-27 2.1283524211072966E-4 28-29 1.0641762105536483E-4 30-31 4.256704842214593E-4 32-33 0.00329894625271631 34-35 6.38505726332189E-4 36-37 0.0011705938316090131 38-39 0.004788792947491417 40-41 1.0641762105536483E-4 42-43 0.003192528631660945 44-45 0.00659789250543262 46-47 0.0 48-49 0.0029796933895502153 50-51 0.0014898466947751076 52-53 0.0010641762105536483 54-55 2.1283524211072966E-4 56-57 0.014047125979308158 58-59 0.0035117814948270394 60-61 0.0056401339159343365 62-63 3.192528631660945E-4 64-65 0.005533716294878971 66-67 0.0019155171789965672 68-69 0.00329894625271631 70-71 0.00659789250543262 72-73 0.004575957705380688 74-75 0.009364750652872105 76-77 0.0014898466947751076 78-79 0.008087739200207727 80-81 0.003724616736937769 82-83 0.0056401339159343365 84-85 0.003618199115882404 86-87 0.0010641762105536483 88-89 0.0043631224632699585 90-91 0.011705938316090132 92-93 0.004788792947491417 94-95 4.256704842214593E-4 96-97 1.0641762105536483E-4 98-99 0.0013834290737197428 100-101 0.0035117814948270394 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 469847.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.53510176964474 #Duplication Level Percentage of deduplicated Percentage of total 1 83.29812071406421 43.76075248933516 2 9.728087822166605 10.221321675231287 3 2.7653572709291625 4.358349769730718 4 1.2340363529239944 2.5932090155321315 5 0.6732940838880028 1.7685786608977978 6 0.4496678931960605 1.4174009118958069 7 0.31173335867825763 1.1463860606208802 8 0.1968519365789228 0.827330921738029 9 0.17057726629406852 0.8065164639912014 >10 1.0433748416548423 10.18052623204962 >50 0.06931332263041587 2.4910463156558214 >100 0.04661422281961273 4.597511536435967 >500 0.003648069611969692 1.4625154808819925 >1k 0.00851216242792928 10.110053506964816 >5k 4.0534106799663245E-4 1.1982490697325234 >10k+ 4.0534106799663245E-4 3.0602518893062194 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14371 3.058655264373296 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5627 1.1976239073570758 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4767 1.0145855991418482 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3952 0.8411248768216035 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3822 0.8134562953472088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3190 0.6789444223332277 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3113 0.6625561086907014 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3040 0.6470191360166182 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2936 0.6248842708371023 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2851 0.6067932752576902 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2849 0.6063676047734688 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2387 0.5080377229183117 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1823 0.3879986463678602 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1787 0.3803365776518739 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1539 0.327553437608413 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1466 0.3120164649343297 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1362 0.2898815997548138 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1217 0.259020489648758 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1111 0.23645995398502065 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1088 0.23156474341647384 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1068 0.22730803857425927 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1060 0.22560535663737344 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1049 0.22326416897415544 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 974 0.20730152581585068 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 971 0.20666302008951848 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 893 0.1900618712048816 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 758 0.1613291135199331 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.15409271528816826 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 708 0.1506873514143966 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 668 0.14217394172996742 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 643 0.13685306067719918 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 529 0.11258984307657598 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 478 0.10173524572892878 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.0641762105536483E-4 0.0 24-25 0.0 0.0 0.0 6.38505726332189E-4 0.0 26-27 0.0 0.0 0.0 0.002021934800051932 0.0 28-29 0.0 0.0 0.0 0.004788792947491417 0.0 30-31 0.0 0.0 0.0 0.00947116827392747 0.0 32-33 0.0 0.0 0.0 0.015217719810917171 0.0 34-35 0.0 0.0 0.0 0.02181561231634979 0.0 36-37 0.0 0.0 0.0 0.028094251958616315 0.0 38-39 0.0 0.0 0.0 0.041183619348426195 0.0 40-41 0.0 0.0 0.0 0.06555325457010473 0.0 42-43 0.0 0.0 0.0 0.10833313823436139 0.0 44-45 0.0 0.0 0.0 0.14909108709856614 0.0 46-47 0.0 0.0 0.0 0.23901397689034942 0.0 48-49 0.0 0.0 0.0 0.3270213495031361 0.0 50-51 0.0 0.0 0.0 0.3945965388732928 0.0 52-53 0.0 0.0 0.0 0.46834395026466064 0.0 54-55 0.0 0.0 0.0 0.5473058250877414 0.0 56-57 0.0 0.0 0.0 0.699802276060079 0.0 58-59 0.0 0.0 0.0 0.8513409684429186 0.0 60-61 0.0 0.0 0.0 1.0141599286576268 0.0 62-63 0.0 0.0 0.0 1.1707002492300684 0.0 64-65 0.0 0.0 0.0 1.3428839600976488 0.0 66-67 0.0 0.0 0.0 1.6099921889466144 0.0 68-69 0.0 0.0 0.0 1.9226471596072763 0.0 70-71 0.0 0.0 0.0 2.178262285382263 0.0 72-73 0.0 0.0 0.0 2.395992738061539 0.0 74-75 0.0 0.0 0.0 2.642455948425764 0.0 76-77 0.0 0.0 0.0 2.9147786407064427 0.0 78-79 0.0 0.0 0.0 3.239352384925305 0.0 80-81 0.0 0.0 0.0 3.668853903504758 0.0 82-83 0.0 0.0 0.0 4.099951686400041 0.0 84-85 0.0 0.0 0.0 4.571488165296362 0.0 86-87 0.0 0.0 0.0 5.040896291771577 0.0 88-89 0.0 0.0 0.0 5.636515716818454 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCGTA 45 9.6222805E-4 42.219524 9 GTAAGCG 105 0.0 36.188168 94-95 GTACACG 80 4.4857242E-4 29.691923 1 GCACCGT 65 0.0058424245 29.2289 6 TACCTGG 380 0.0 27.501171 2 TACGATT 70 0.008389222 27.141123 4 ACGTAGT 35 0.008393419 27.141123 46-47 GAGTCGT 45 8.944105E-4 26.39001 84-85 TGTAGGG 110 9.4621966E-5 25.910194 2 ATAGTGC 95 0.0012259315 24.998404 6 TAAGGTA 115 1.2807325E-4 24.781025 4 GCTTATG 120 1.706993E-4 23.75354 1 CTAAGGG 120 1.7082258E-4 23.751013 2 GCCGCGA 50 0.0016530117 23.751013 62-63 GTACATG 4635 0.0 23.676666 1 GAGTACT 4460 0.0 23.644506 12-13 GTGTAAG 225 2.7976057E-9 23.225683 1 TACATGG 4695 0.0 23.16925 2 AGTACTT 4460 0.0 23.005465 12-13 AGGCTTA 125 2.2541218E-4 22.798544 6 >>END_MODULE