##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544942 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.509523949337726 31.0 31.0 34.0 30.0 34.0 2 31.493769979190446 31.0 31.0 34.0 30.0 34.0 3 31.51131496562937 31.0 31.0 34.0 28.0 34.0 4 35.17218529678388 37.0 35.0 37.0 33.0 37.0 5 35.12995511448925 37.0 35.0 37.0 32.0 37.0 6 35.287190196387876 37.0 35.0 37.0 33.0 37.0 7 35.12562254331654 37.0 35.0 37.0 32.0 37.0 8 35.13778347053448 37.0 35.0 37.0 32.0 37.0 9 36.63066161169446 39.0 37.0 39.0 32.0 39.0 10-11 36.497870415567164 39.0 37.0 39.0 32.0 39.0 12-13 36.41910607000378 39.0 37.0 39.0 32.0 39.0 14-15 37.37514909843617 39.5 37.0 41.0 32.0 41.0 16-17 37.378021881227724 39.0 37.0 41.0 32.0 41.0 18-19 37.47362196343831 40.0 37.0 41.0 32.0 41.0 20-21 37.41948776200036 40.0 37.0 41.0 32.0 41.0 22-23 37.31135974103666 39.5 36.5 41.0 32.0 41.0 24-25 37.108651012401324 39.0 36.0 41.0 31.0 41.0 26-27 36.90588356192035 39.0 36.0 41.0 30.5 41.0 28-29 36.963530981278744 39.0 36.0 41.0 31.0 41.0 30-31 36.829227697626536 39.0 36.0 41.0 31.0 41.0 32-33 36.52421266850417 39.0 35.0 41.0 30.0 41.0 34-35 36.23096402919944 38.0 35.0 40.0 30.0 41.0 36-37 35.90695982324725 38.0 35.0 40.0 29.5 41.0 38-39 35.66808761299367 38.0 35.0 40.0 29.0 41.0 40-41 35.52363003769209 38.0 35.0 40.0 28.5 41.0 42-43 35.13600805223308 38.0 35.0 40.0 26.5 41.0 44-45 34.65785900150841 38.0 34.0 40.0 24.5 41.0 46-47 34.030779422397245 38.0 33.0 40.0 22.0 41.0 48-49 33.535119333800665 38.0 33.0 40.0 18.0 41.0 50-51 32.72832521626155 37.0 32.0 39.5 14.5 40.5 52-53 32.90699927698728 37.5 33.0 39.5 13.5 40.5 54-55 33.252707627600735 38.0 33.0 40.0 11.5 41.0 56-57 33.07509790032701 38.0 33.0 40.0 8.5 41.0 58-59 32.80145318217352 37.0 32.5 40.0 7.5 41.0 60-61 32.41205853099963 37.0 32.0 40.0 6.5 41.0 62-63 32.14105996601474 36.0 31.0 40.0 2.0 41.0 64-65 31.849906045046996 36.0 31.0 39.0 2.0 41.0 66-67 31.380529487541793 35.0 31.0 39.0 2.0 40.5 68-69 30.94523545625039 35.0 30.5 38.5 2.0 40.0 70-71 30.43878155840438 35.0 29.5 37.5 2.0 40.0 72-73 30.02259873527825 35.0 29.0 37.0 2.0 39.5 74-75 29.638064234358886 35.0 29.0 36.5 2.0 39.0 76-77 29.22291363117543 34.0 29.0 36.0 2.0 39.0 78-79 28.74018978166484 34.0 28.5 36.0 2.0 37.5 80-81 28.23355329558008 34.0 27.0 35.0 2.0 37.0 82-83 27.79769039640916 34.0 26.0 35.0 2.0 37.0 84-85 27.410152823603248 34.0 26.0 35.0 2.0 36.0 86-87 27.15145373269082 34.0 26.0 35.0 2.0 36.0 88-89 26.725595567968703 33.0 25.0 35.0 2.0 35.5 90-91 26.276769821375485 33.0 24.0 35.0 2.0 35.0 92-93 25.85484877289693 33.0 22.0 35.0 2.0 35.0 94-95 25.401060112819344 33.0 19.5 35.0 2.0 35.0 96-97 24.811433143343695 32.0 14.0 35.0 2.0 35.0 98-99 24.17512505918061 32.0 2.0 35.0 2.0 35.0 100-101 22.32939101775969 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.307031356954127 1101 2 0.2528338989333925 1101 3 0.2499013780863315 1101 4 0.377664307803002 1101 5 0.3027428887737429 1101 6 0.2843671054467123 1101 7 0.38351197532949044 1101 8 0.24465827151505692 1101 9 0.5233012400159112 1101 10-11 0.4563376111444626 1101 12-13 0.4767170131835883 1101 14-15 0.7199180025249703 1101 16-17 0.6943338346733015 1101 18-19 0.7477322549441041 1101 20-21 0.520082277633847 1101 22-23 0.6292665518911278 1101 24-25 0.599916178037347 1101 26-27 0.6981291513724415 1101 28-29 0.7564040725447327 1101 30-31 0.5768126642110118 1101 32-33 0.550192407882669 1101 34-35 0.6822759554719227 1101 36-37 0.8163190258662922 1101 38-39 0.9054542057364969 1101 40-41 0.8241441164667478 1101 42-43 0.7126546830977389 1101 44-45 0.7337940235690965 1101 46-47 1.0578918219612845 1101 48-49 0.8698221077128707 1101 50-51 1.1541195700036795 1101 52-53 1.1671562077830515 1101 54-55 1.1863418216803723 1101 56-57 1.1014439014817725 1101 58-59 1.219569773832042 1101 60-61 1.1517859334085259 1101 62-63 1.0787446918482182 1101 64-65 1.0873487665846504 1101 66-67 1.0762559187556704 1101 68-69 1.0092841404786164 1101 70-71 1.0489452137752373 1101 72-73 1.1109785219269277 1101 74-75 1.0019632701641719 1101 76-77 1.0007593202202223 1101 78-79 1.1749391461456007 1101 80-81 1.038157979085959 1101 82-83 0.908552681125844 1101 84-85 0.8981682037475984 1101 86-87 0.9402846799314162 1101 88-89 0.9332807668920111 1101 90-91 0.9445387503997047 1101 92-93 0.7855887939914972 1101 94-95 0.5849857514818915 1101 96-97 0.6228711411945653 1101 98-99 0.6747085643579602 1101 100-101 1.0686470778342887 1102 1 -0.15936012048815584 1102 2 -0.061583778286433244 1102 3 0.06856049612436621 1102 4 0.0315532419067992 1102 5 0.13362105268682 1102 6 -0.0128601469338534 1102 7 -0.20287421196874078 1102 8 -0.19956970656475193 1102 9 0.13301747126302388 1102 10-11 0.12946774868820654 1102 12-13 0.16931004850546572 1102 14-15 0.27102850888670815 1102 16-17 -0.09120508179668008 1102 18-19 -0.051543656588407316 1102 20-21 -0.2753404760896032 1102 22-23 -0.6682489455180516 1102 24-25 -0.34155227778316544 1102 26-27 -0.2075956492298232 1102 28-29 -0.034927721221038155 1102 30-31 -0.17483796246759198 1102 32-33 -0.24273321133548365 1102 34-35 -0.534386811409064 1102 36-37 -0.5371329626852983 1102 38-39 -0.3282421467769936 1102 40-41 -0.7765441109280644 1102 42-43 -0.21374110106438593 1102 44-45 -0.33246715016466055 1102 46-47 -0.12381657241217425 1102 48-49 -0.11900246297158645 1102 50-51 -0.26233472789925116 1102 52-53 -0.13614919969368344 1102 54-55 -0.07022634996445731 1102 56-57 -0.04270235065462202 1102 58-59 -0.05983206686680376 1102 60-61 -0.496003227221955 1102 62-63 -0.24853846311230754 1102 64-65 0.011512771223699758 1102 66-67 -1.4893664705795118E-4 1102 68-69 -0.13354423843133745 1102 70-71 -0.09722977469496286 1102 72-73 -0.0652243923998661 1102 74-75 -0.06743256825583543 1102 76-77 0.2330220776060763 1102 78-79 0.2396196350091735 1102 80-81 0.14772033835456 1102 82-83 0.248044284717718 1102 84-85 0.11798004756627378 1102 86-87 0.0646958970714877 1102 88-89 -0.04881752127162997 1102 90-91 -0.3603418313804987 1102 92-93 -0.41443375694309736 1102 94-95 0.0168581911878114 1102 96-97 -0.013955382773858105 1102 98-99 -0.02871146290662452 1102 100-101 0.18073285277257156 1103 1 0.18315881890578112 1103 2 0.27459046413780897 1103 3 0.39450746582133434 1103 4 0.3395077873613417 1103 5 0.2674278708686373 1103 6 0.10011333791463528 1103 7 0.1432432122736813 1103 8 0.060941657071609256 1103 9 0.40299853186908763 1103 10-11 0.310386309294266 1103 12-13 0.28053163941456205 1103 14-15 0.4693239634321671 1103 16-17 0.5397134788093823 1103 18-19 0.29907187371462385 1103 20-21 0.33554967542555403 1103 22-23 0.08345559993649232 1103 24-25 0.6166863585804663 1103 26-27 0.45348389622472496 1103 28-29 0.4492578848395681 1103 30-31 0.4569328708657423 1103 32-33 0.38643345533118634 1103 34-35 0.2996094007121428 1103 36-37 0.34856779489045664 1103 38-39 0.2827427017078534 1103 40-41 0.2088725557386013 1103 42-43 0.39141988378410275 1103 44-45 0.6970783043807955 1103 46-47 0.6814864578908626 1103 48-49 0.7781092794526572 1103 50-51 0.29031678725225873 1103 52-53 0.588756103336614 1103 54-55 0.9629649379143288 1103 56-57 0.7763790887393114 1103 58-59 0.7348175543453195 1103 60-61 0.9233149545962291 1103 62-63 0.9410922187058723 1103 64-65 0.8124597409206764 1103 66-67 0.7612146997165823 1103 68-69 0.8742209130838106 1103 70-71 0.8660278010626179 1103 72-73 0.7392737136607401 1103 74-75 0.7014121287138586 1103 76-77 0.5866679109394077 1103 78-79 0.8043450137970503 1103 80-81 1.0255138989606216 1103 82-83 0.8961976938086273 1103 84-85 1.022383456657181 1103 86-87 0.8388909728290628 1103 88-89 0.7957374029707935 1103 90-91 0.9131903655891982 1103 92-93 1.084382530935688 1103 94-95 1.090390388157509 1103 96-97 1.1934783293473572 1103 98-99 0.9402752795176177 1103 100-101 0.7920491406513612 1104 1 0.15299979523714313 1104 2 0.3809390834671973 1104 3 0.18252521730062554 1104 4 0.29258964140473154 1104 5 0.30091529690414376 1104 6 0.22022551740181484 1104 7 0.28284503673127404 1104 8 0.3999887478013946 1104 9 0.5051619934075475 1104 10-11 0.48898899667099727 1104 12-13 0.6111991690003578 1104 14-15 0.42983308572013357 1104 16-17 0.23241873028867133 1104 18-19 0.5604469969887873 1104 20-21 0.43317048410405334 1104 22-23 0.40133035338816825 1104 24-25 0.4358000173575931 1104 26-27 0.5214942512543104 1104 28-29 0.2748187822754673 1104 30-31 0.046574759425894285 1104 32-33 0.06834913580456004 1104 34-35 0.34073982477526243 1104 36-37 0.5590152800975545 1104 38-39 0.5726465478617015 1104 40-41 0.32223547487075166 1104 42-43 0.42914485912927347 1104 44-45 0.498255572625375 1104 46-47 0.5491331541433269 1104 48-49 0.4676772035157697 1104 50-51 0.21948961762701202 1104 52-53 0.44406921970742985 1104 54-55 0.8075254655277391 1104 56-57 0.5423030285815216 1104 58-59 0.7121073718995561 1104 60-61 0.7435935542017518 1104 62-63 0.4459831210727394 1104 64-65 0.5302913484157337 1104 66-67 0.5965019285134261 1104 68-69 0.55057076515482 1104 70-71 0.6816712921868735 1104 72-73 0.2869099316094541 1104 74-75 0.41178564942391915 1104 76-77 0.483983616067615 1104 78-79 0.5905722080771305 1104 80-81 0.824506132688434 1104 82-83 0.6383975212247996 1104 84-85 0.7078586785506715 1104 86-87 0.715595238115057 1104 88-89 0.5850402875861782 1104 90-91 0.5367696308751952 1104 92-93 0.41214079030452666 1104 94-95 0.30662867711214403 1104 96-97 0.5617506419706295 1104 98-99 0.3993328485511469 1104 100-101 0.4917748556414985 1105 1 0.14918007250808785 1105 2 0.23871750544424586 1105 3 0.21792245141211453 1105 4 0.27489443481571385 1105 5 0.35526108412512514 1105 6 -0.028521988694393485 1105 7 0.17708987413727328 1105 8 -0.23945159460946286 1105 9 0.168407393540555 1105 10-11 0.31567419354018256 1105 12-13 -0.01270052917333686 1105 14-15 0.2707979067558952 1105 16-17 0.07602922088431541 1105 18-19 0.060696813676202055 1105 20-21 -0.12056824004034894 1105 22-23 -0.45003383330077895 1105 24-25 -0.39409954439743444 1105 26-27 -0.3020456426801772 1105 28-29 -0.10234814301825423 1105 30-31 -0.007961161314852916 1105 32-33 -0.26347648703193016 1105 34-35 -0.47953625250591614 1105 36-37 -0.30072074977812946 1105 38-39 -0.404198940943445 1105 40-41 -0.3067134192854084 1105 42-43 -0.048657265591501186 1105 44-45 0.12881761754121612 1105 46-47 -0.2556022789006889 1105 48-49 0.0031648149954008886 1105 50-51 0.021893996666285886 1105 52-53 -0.0850851550687608 1105 54-55 0.45352839852912297 1105 56-57 0.6907597923896418 1105 58-59 0.5982736662569437 1105 60-61 0.6642311409555006 1105 62-63 0.5676907417606252 1105 64-65 0.7623846928899276 1105 66-67 0.3425517554322397 1105 68-69 0.3856312156774706 1105 70-71 0.4060314033680896 1105 72-73 0.6547441147174133 1105 74-75 0.7304016820279813 1105 76-77 0.6463866309201975 1105 78-79 0.7779296079373026 1105 80-81 0.777355577634971 1105 82-83 0.5776443196491634 1105 84-85 0.481997109394932 1105 86-87 0.782279241320893 1105 88-89 0.8606554505212252 1105 90-91 0.9312634323519298 1105 92-93 0.6317603527416473 1105 94-95 0.24096525607297536 1105 96-97 0.5053824479832798 1105 98-99 0.4426543020920626 1105 100-101 0.38939093940255987 1106 1 0.053402908222768275 1106 2 0.08262096618100756 1106 3 0.212239514858112 1106 4 0.391952329100981 1106 5 0.42115488487915087 1106 6 0.48335363271907283 1106 7 0.5056100394774177 1106 8 0.6249175035397911 1106 9 0.5662154758270574 1106 10-11 0.5086669207945533 1106 12-13 0.5912994809206822 1106 14-15 0.39899960558044967 1106 16-17 0.43919082119116837 1106 18-19 0.47324091195164186 1106 20-21 0.4969514559275936 1106 22-23 0.4881768492067735 1106 24-25 0.3589349721239614 1106 26-27 0.13331095401807147 1106 28-29 0.27014412243443076 1106 30-31 0.2343673790385381 1106 32-33 0.44795928720508726 1106 34-35 0.46409239837706195 1106 36-37 0.605129747604991 1106 38-39 0.5228149433891147 1106 40-41 0.32464897138366666 1106 42-43 0.3121902792890623 1106 44-45 0.30991579708365435 1106 46-47 0.5301091228062162 1106 48-49 0.14111734279182286 1106 50-51 0.4866809874857694 1106 52-53 0.24113861939031977 1106 54-55 0.4285671343533224 1106 56-57 0.5822578467661685 1106 58-59 0.28622316584560537 1106 60-61 0.7933930339891049 1106 62-63 0.8293228448004406 1106 64-65 0.4700907245750763 1106 66-67 0.4907371019349114 1106 68-69 0.5719606241170858 1106 70-71 0.5478688693638425 1106 72-73 0.8467779095159607 1106 74-75 0.5262096330921437 1106 76-77 0.4089196257671972 1106 78-79 0.5518940870603295 1106 80-81 0.526545532060446 1106 82-83 0.40552982311394103 1106 84-85 0.3954761668147988 1106 86-87 0.8727305452166831 1106 88-89 0.6203898767010898 1106 90-91 0.8173725386773505 1106 92-93 0.7246745989450325 1106 94-95 0.5764957223664986 1106 96-97 0.4166786723129121 1106 98-99 0.5828477036273618 1106 100-101 0.7780285992036049 1107 1 0.050580454991408175 1107 2 0.0317578953304718 1107 3 0.17807015695283468 1107 4 0.1274665029576738 1107 5 0.175044461082706 1107 6 0.2847750351623404 1107 7 0.44413197374157676 1107 8 0.1611996845945427 1107 9 0.21426573370394664 1107 10-11 0.3315111181627657 1107 12-13 0.23188389476624138 1107 14-15 0.1775521744413382 1107 16-17 0.2851502142833837 1107 18-19 0.6563683186687612 1107 20-21 0.7293379017252448 1107 22-23 0.44649076813220745 1107 24-25 0.669142398335822 1107 26-27 0.5061023977537715 1107 28-29 0.47279572887626387 1107 30-31 0.32755118126329563 1107 32-33 0.4716207641996917 1107 34-35 0.3787091713543447 1107 36-37 0.4261958529944394 1107 38-39 0.4356670136344505 1107 40-41 0.5366248493165813 1107 42-43 0.5283703807259954 1107 44-45 0.45727516982422145 1107 46-47 0.6606435679826106 1107 48-49 0.6989187213486758 1107 50-51 0.8736596771605178 1107 52-53 0.7499755222968645 1107 54-55 0.4143745327155557 1107 56-57 0.7411105489839613 1107 58-59 0.8613609411954783 1107 60-61 0.7912239760029536 1107 62-63 0.9828305596844693 1107 64-65 0.5237842822050744 1107 66-67 0.5133649290743776 1107 68-69 0.22788108127953066 1107 70-71 0.021245690970872033 1107 72-73 0.07385418002159483 1107 74-75 -0.25731302419133684 1107 76-77 -0.06609871750096019 1107 78-79 0.16255888994384193 1107 80-81 0.08605002281383278 1107 82-83 0.09750216628568964 1107 84-85 0.5509146110914322 1107 86-87 0.39881547588716515 1107 88-89 0.19614355740504408 1107 90-91 0.4661672386778868 1107 92-93 0.8248020645748362 1107 94-95 0.16260464656729212 1107 96-97 0.2033263798060716 1107 98-99 -0.06290228164446532 1107 100-101 0.3714212736788838 1108 1 0.2184266760486402 1108 2 -0.04249286919552375 1108 3 -0.17334967703580872 1108 4 -0.032516022162468516 1108 5 0.1289457280114945 1108 6 0.036928814105110064 1108 7 0.5866658313213051 1108 8 0.43775713326208887 1108 9 0.432194960440512 1108 10-11 -0.13325952403906172 1108 12-13 0.04941556798598867 1108 14-15 0.27607991581312064 1108 16-17 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-2.578920570903385 2316 92-93 -2.658248231475657 2316 94-95 -3.1738252388573187 2316 96-97 -3.524472790897647 2316 98-99 -3.9157702153244216 2316 100-101 -4.3447547358799135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1176.0 3 469.0 4 401.0 5 480.0 6 542.0 7 624.0 8 814.0 9 1014.0 10 1397.0 11 2155.0 12 3081.0 13 3846.0 14 4486.0 15 5604.0 16 7593.0 17 9856.0 18 8301.0 19 4767.0 20 4454.0 21 4624.0 22 4910.0 23 5410.0 24 6221.0 25 6887.0 26 7918.0 27 9380.0 28 10878.0 29 12881.0 30 15643.0 31 19067.0 32 23537.0 33 29534.0 34 39537.0 35 54379.0 36 70633.0 37 85315.0 38 65759.0 39 11364.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.30001266387327 18.047120620639912 14.295677581457152 19.35718913402966 2 15.132140360020774 21.75429839258744 38.76582133294673 24.347739914445057 3 21.83408117011222 27.322560813352787 30.303997944595846 20.539360071939143 4 11.43292459064542 16.290292973052352 39.38835450473603 32.88842793156619 5 11.453009340853013 39.47556838209376 33.597329925978144 15.47409235107508 6 27.807860743107387 36.33863075072759 18.22238135896659 17.631127147198427 7 25.603956070582235 35.89777948283996 21.030575476841236 17.467688969736574 8 26.28267254038179 29.353891336270195 20.436490455212923 23.926945668135097 9 26.582215753606935 17.60254600782229 22.948575120261797 32.86666311830898 10-11 24.873869970404424 26.00105383751074 25.865468645580126 23.25960754650471 12-13 28.60531357197868 24.94604823393449 26.486347059250097 19.962291134836732 14-15 24.17855520426565 24.819687326490065 29.874591032777033 21.127166436467256 16-17 19.6922195872277 29.2533645127422 29.13212825162811 21.922287648401994 18-19 19.14869402967952 27.987018767902654 32.805603663086366 20.05868353933146 20-21 21.742510035258768 24.702704885386055 31.662136193190893 21.89264888616428 22-23 20.20091528686911 25.263807993480853 32.77431764143403 21.760959078216008 24-25 21.89388475676112 24.93489834231635 32.424754595654015 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19.29595492937069 27.481522601837877 32.06511017621772 21.157412292573717 52-53 20.720816607409105 28.769996559238443 28.67796765684138 21.83121917651107 54-55 19.428405221573723 26.896499835300403 30.294266343567955 23.380828599557926 56-57 21.439850696830703 26.508469413128072 28.805914137832 23.24576575220922 58-59 22.98951506126078 26.37191023710624 28.650482773301622 21.988091928331354 60-61 21.500539052643653 26.639660511526554 31.39341667622434 20.46638375960546 62-63 20.64932753200852 27.358500953328207 30.6472538825874 21.34491763207587 64-65 19.85865905948266 29.182536631288613 29.7511485557147 21.207655753514025 66-67 20.675534447907673 27.47620572493974 28.372556917704333 23.475702909448252 68-69 20.07674570324077 27.29253013993887 29.610224685800485 23.020499471019875 70-71 22.024013249955267 27.13589003638232 28.531539756747705 22.308556956914703 72-73 21.199411662695177 26.202113866941446 30.81935053507314 21.779123935290237 74-75 19.83195960570974 29.41028043128594 29.162725018052825 21.595034944951493 76-77 20.040408934007917 27.570747748789294 29.065621392878704 23.323221924324088 78-79 20.599462343386172 27.42782983324201 29.605531860605776 22.36717596276604 80-81 21.70401422810859 27.39911202800103 28.462457579055755 22.43441616483462 82-83 20.633791896705336 26.702133223471424 30.331338263050156 22.33273661677309 84-85 20.482438472486162 27.60982091160097 29.33555515384975 22.572185462063118 86-87 20.274834628502532 28.333879295552748 29.061431301895084 22.329854774049636 88-89 19.845500115154625 28.008540711059155 27.564621930395223 24.581337243390998 90-91 20.02628094365472 26.82895271724219 28.45591197352085 24.68885436558224 92-93 21.320157029146564 27.777879739459355 28.929903994919858 21.972059236474223 94-95 20.49488316099806 27.09956479570723 28.93801160361266 23.467540439682043 96-97 18.50712031123835 27.556705571460032 30.795804888791018 23.140369228510608 98-99 19.87493105514762 28.557439665797574 27.789295353782308 23.7783339252725 100-101 19.165513164609944 27.003054791653664 29.19969598948447 24.631736054251924 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 704.0 1 508.0 2 618.5 3 843.0 4 631.0 5 573.0 6 868.0 7 1543.0 8 2399.5 9 2730.0 10 2199.0 11 1673.5 12 1612.5 13 1680.0 14 1741.0 15 1729.5 16 1718.5 17 1765.0 18 1845.0 19 1892.5 20 1988.5 21 2198.5 22 2413.5 23 2648.0 24 2989.0 25 3442.0 26 3962.5 27 4605.0 28 5294.0 29 5942.0 30 6726.0 31 7588.5 32 8825.5 33 9680.0 34 10364.0 35 11014.5 36 11670.5 37 12664.5 38 12860.0 39 12619.0 40 12659.5 41 13101.0 42 13548.5 43 14239.5 44 15040.5 45 15805.5 46 16914.5 47 24546.5 48 41734.0 49 40754.5 50 23815.5 51 16139.5 52 13473.5 53 12885.5 54 12682.0 55 11966.0 56 11154.5 57 10071.0 58 9421.0 59 8672.5 60 7691.0 61 7000.0 62 6057.5 63 4472.5 64 3082.5 65 2341.0 66 1693.5 67 1273.5 68 931.0 69 645.5 70 509.0 71 404.0 72 351.5 73 348.5 74 284.0 75 229.0 76 190.5 77 126.0 78 80.0 79 60.0 80 47.5 81 27.5 82 17.0 83 14.5 84 11.0 85 5.5 86 1.5 87 1.0 88 0.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015597990244833396 2 0.005321667260001982 3 0.0067897134006921845 4 0.03339804970070209 5 0.04367437268553351 6 0.06184144367657476 7 0.029177417046217763 8 0.026057818997251084 9 0.016332013315178494 10-11 0.048812534177949216 12-13 0.04450014863967174 14-15 0.022846468064491268 16-17 0.025323795926905982 18-19 0.028535146859665798 20-21 0.020736151737249103 22-23 0.015689743128626532 24-25 0.004587644189656881 26-27 0.0032113509327598164 28-29 0.0019268105596558901 30-31 0.006514454749312771 32-33 0.0012845403731039267 34-35 0.0030278451651735414 36-37 0.003945374003104917 38-39 0.004954655724829431 40-41 0.0025690807462078534 42-43 7.340230703451009E-4 44-45 0.0014680461406902019 46-47 0.0030278451651735414 48-49 9.175288379313761E-4 50-51 0.0035783624679323668 52-53 8.257759541382386E-4 54-55 0.0019268105596558901 56-57 0.0019268105596558901 58-59 0.0032113509327598164 60-61 8.257759541382386E-4 62-63 0.0016515519082764773 64-65 0.0033948567003460922 66-67 0.0019268105596558901 68-69 0.004679397073450019 70-71 0.0063309489817264955 72-73 0.0030278451651735414 74-75 0.0024773278624147157 76-77 0.002385574978621578 78-79 0.03128773337345993 80-81 0.0192681055965589 82-83 0.0028443393975872665 84-85 0.007982500890002972 86-87 0.005963937446553945 88-89 0.0041288797706911925 90-91 0.011102098938969652 92-93 0.013762932568970643 94-95 0.004679397073450019 96-97 0.004037126886898055 98-99 0.022479456529318716 100-101 0.041105291939325654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 544942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.90020640742475 #Duplication Level Percentage of deduplicated Percentage of total 1 83.6948116675481 45.948624357789306 2 9.541778708345321 10.476912411642575 3 2.7244218414465102 4.4871396430892885 4 1.2087777301404963 2.6544858754164644 5 0.6724948993520468 1.8460054391183849 6 0.4181180039023473 1.3772858830139576 7 0.2902101169365897 1.1152816724939152 8 0.2211924839739116 0.971481042075099 9 0.1585328366849538 0.7833136910722669 >10 0.9475719729639808 9.656535928952632 >50 0.07105222519570295 2.6587582395850133 >100 0.03969584384116114 3.915249489584866 >500 0.0033357851967362297 1.3257164065253215 >1k 0.006337991873798837 6.6910311685052 >5k 0.0016678925983681149 6.092178751135702 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8127 1.4913513731736587 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8004 1.468780163760547 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6193 1.1364512186618025 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5699 1.0457993694741825 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5243 0.9621207394548411 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4798 0.8804606728789487 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3365 0.6174969079278162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3040 0.5578575334622767 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2608 0.4785830418650058 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2524 0.4631685573877587 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1959 0.35948779870151315 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1901 0.3488444641815092 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1683 0.30884020684770125 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1599 0.2934257223704541 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1490 0.2734235937035501 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1483 0.2721390533304462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1482 0.2719555475628599 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1467 0.26920296104906577 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1251 0.2295657152504303 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1240 0.2275471518069813 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1186 0.21763784035732245 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1176 0.21580278268145967 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.2141512307731832 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1117 0.20497594239386943 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 898 0.16478817929247516 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 873 0.1602005351028183 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 831 0.15249329286419472 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 779 0.14295099294970842 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 686 0.1258849565641848 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 686 0.1258849565641848 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 637 0.11689317395245732 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 634 0.11634265664969849 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 623 0.11432409320624946 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.10863541441107495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.175288379313762E-5 0.0 22-23 0.0 0.0 0.0 5.505173027588258E-4 0.0 24-25 0.0 0.0 0.0 0.0014680461406902019 0.0 26-27 0.0 0.0 0.0 0.002385574978621578 0.0 28-29 0.0 0.0 0.0 0.004771149957243157 0.0 30-31 0.0 0.0 0.0 0.009083535495520625 0.0 32-33 0.0 0.0 0.0 0.013671179685177505 0.0 34-35 0.0 0.0 0.0 0.0192681055965589 0.0 36-37 0.0 0.0 0.0 0.02569080746207853 0.0 38-39 0.0 0.0 0.0 0.03789394100656584 0.0 40-41 0.0 0.0 0.0 0.05963937446553945 0.0 42-43 0.0 0.0 0.0 0.09166113090934447 0.0 44-45 0.0 0.0 0.0 0.1282705315428064 0.0 46-47 0.0 0.0 0.0 0.20314088471800668 0.0 48-49 0.0 0.0 0.0 0.2756256629145854 0.0 50-51 0.0 0.0 0.0 0.33159492202839935 0.0 52-53 0.0 0.0 0.0 0.3928858484022153 0.0 54-55 0.0 0.0 0.0 0.4505066594243057 0.0 56-57 0.0 0.0 0.0 0.5712534544960748 0.0 58-59 0.0 0.0 0.0 0.7003497619930195 0.0 60-61 0.0 0.0 0.0 0.8350429954013454 0.0 62-63 0.0 0.0 0.0 0.9720300509044999 0.0 64-65 0.0 0.0 0.0 1.1217707572549005 0.0 66-67 0.0 0.0 0.0 1.3411519024042926 0.0 68-69 0.0 0.0 0.0 1.6072352654043915 0.0 70-71 0.0 0.0 0.0 1.8123947135658474 0.0 72-73 0.0 0.0 0.0 1.9883767446810854 0.0 74-75 0.0 0.0 0.0 2.1932609341911617 0.0 76-77 0.0 0.0 0.0 2.418055499484349 0.0 78-79 0.0 0.0 0.0 2.6780831721541007 0.0 80-81 0.0 0.0 0.0 3.039681287182856 0.0 82-83 0.0 0.0 0.0 3.4027474483523017 0.0 84-85 0.0 0.0 0.0 3.8414179857672925 0.0 86-87 0.0 0.0 0.0 4.318808240142988 1.8350576758627523E-4 88-89 0.0 0.0 0.0 4.895750373434238 1.8350576758627523E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 55 0.0025727644 34.560978 4 CCCTATA 75 3.068242E-4 31.680897 2 TACCTGG 435 0.0 29.496006 2 GTACATG 5235 0.0 28.685284 1 TACATGG 5235 0.0 27.505192 2 ACATGGG 5135 0.0 26.467585 3 AGGTAAG 180 6.6502253E-9 26.400747 2 GTCGGGA 205 9.495125E-10 25.499258 2 CATGGGA 3870 0.0 25.418396 4 GGTAAGG 225 1.0186341E-10 25.344717 3 GAGTACT 4220 0.0 25.048786 12-13 CATGGGT 325 0.0 24.857319 4 GGGATTG 235 1.7462298E-10 24.261765 7 AGTACTT 4395 0.0 24.051395 12-13 ATGGGAT 1460 0.0 23.760674 5 ACTAGAC 100 0.0016482166 23.760672 3 ATGGGAC 625 0.0 23.570587 5 CATGGGG 1090 0.0 23.542685 4 GTACCTG 585 0.0 22.745258 1 GTAAGGT 230 3.597961E-9 22.727598 4 >>END_MODULE