##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 544942 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.437591523501585 31.0 31.0 34.0 30.0 34.0 2 31.60689027456133 31.0 31.0 34.0 30.0 34.0 3 31.835068686208807 31.0 31.0 34.0 30.0 34.0 4 35.64420984251535 37.0 35.0 37.0 33.0 37.0 5 35.50005688678795 37.0 35.0 37.0 33.0 37.0 6 35.653750307372164 37.0 35.0 37.0 33.0 37.0 7 35.596702034344936 37.0 35.0 37.0 33.0 37.0 8 35.58947557721739 37.0 35.0 37.0 33.0 37.0 9 37.159424305705926 39.0 37.0 39.0 33.0 39.0 10-11 37.0377618168539 39.0 37.0 39.0 33.0 39.0 12-13 36.92081083858466 39.0 37.0 39.0 33.0 39.0 14-15 37.8144013491344 39.5 37.0 41.0 33.0 41.0 16-17 37.826997185021526 39.0 37.0 41.0 32.5 41.0 18-19 38.015925548039974 40.0 37.0 41.0 33.0 41.0 20-21 38.118812644281405 40.0 37.0 41.0 33.0 41.0 22-23 38.00464361344878 40.0 37.0 41.0 33.0 41.0 24-25 37.83477966462486 40.0 37.0 41.0 32.5 41.0 26-27 37.52768184504039 39.0 36.5 41.0 32.0 41.0 28-29 37.436978614237844 39.0 36.0 41.0 32.0 41.0 30-31 37.34944085792617 39.0 36.0 41.0 32.0 41.0 32-33 37.020166366328894 39.0 35.0 41.0 31.5 41.0 34-35 36.710801332985895 39.0 35.0 41.0 31.0 41.0 36-37 36.39103060509192 38.5 35.0 40.0 30.5 41.0 38-39 36.156806595931315 38.0 35.0 40.0 30.0 41.0 40-41 35.939644953040876 38.0 35.0 40.0 30.0 41.0 42-43 35.57070935989518 38.0 35.0 40.0 28.5 41.0 44-45 35.08137746769381 38.0 34.5 40.0 26.5 41.0 46-47 34.39648531403342 38.0 34.0 40.0 23.5 41.0 48-49 33.63808809010867 38.0 33.0 40.0 19.0 41.0 50-51 33.36478102256754 38.0 33.0 40.0 16.0 41.0 52-53 33.12765578722139 38.0 33.0 40.0 13.5 41.0 54-55 32.861328361550406 37.0 32.5 40.0 9.5 41.0 56-57 32.509968950824124 37.0 32.0 40.0 8.0 41.0 58-59 32.11417453600566 36.0 31.0 39.5 6.5 41.0 60-61 31.602216382660906 36.0 31.0 39.0 2.0 40.0 62-63 31.386288816057487 35.0 31.0 39.0 2.0 40.0 64-65 30.97923632239761 35.0 30.0 38.5 2.0 40.0 66-67 30.527788278385593 35.0 29.5 38.0 2.0 40.0 68-69 29.971739194262874 34.5 29.0 37.0 2.0 39.5 70-71 29.40253549919074 34.0 28.5 36.5 2.0 39.0 72-73 28.955111736661884 34.0 28.0 36.0 2.0 39.0 74-75 28.623476443364616 34.0 27.5 36.0 2.0 38.5 76-77 27.27439892685827 31.5 26.0 34.5 2.0 36.0 78-79 28.045075439221055 33.5 27.0 35.0 2.0 37.0 80-81 27.797754623427814 33.0 26.0 35.0 2.0 36.5 82-83 27.429305320566225 33.0 26.0 35.0 2.0 36.0 84-85 26.983086273401575 33.0 25.0 35.0 2.0 36.0 86-87 26.680738500611074 33.0 25.0 35.0 2.0 35.0 88-89 26.243546102153992 33.0 24.5 35.0 2.0 35.0 90-91 25.68886688858631 32.0 22.5 34.5 2.0 35.0 92-93 25.214540263000465 32.0 20.0 34.0 2.0 35.0 94-95 24.61264226284632 31.5 17.5 34.0 2.0 35.0 96-97 23.89367675826051 31.0 4.5 34.0 2.0 35.0 98-99 23.113559791684253 31.0 2.0 34.0 2.0 35.0 100-101 20.55468104862536 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37446332359555257 1101 2 0.34940001975323653 1101 3 0.23184973322661762 1101 4 0.2003313799650357 1101 5 0.2511986383502105 1101 6 0.22925772915883869 1101 7 0.1356781814725423 1101 8 0.30136540740515727 1101 9 0.32667771153250413 1101 10-11 0.362716190147502 1101 12-13 0.36341600363365245 1101 14-15 0.34637497424511565 1101 16-17 0.27560600500275 1101 18-19 0.23550364095225973 1101 20-21 0.20196774992264466 1101 22-23 0.12150765240994588 1101 24-25 0.03706088860489842 1101 26-27 0.17525142648506886 1101 28-29 0.15199032421756442 1101 30-31 0.15944915402071302 1101 32-33 0.1723955642378101 1101 34-35 0.4805971959956068 1101 36-37 0.5285466837648869 1101 38-39 0.5530754276316756 1101 40-41 0.6534493885181405 1101 42-43 0.7298514077874856 1101 44-45 0.7799879120534996 1101 46-47 0.6277984966218924 1101 48-49 0.6894597218865215 1101 50-51 0.520308231506327 1101 52-53 0.47089005581354826 1101 54-55 0.644460668306003 1101 56-57 0.698378793590031 1101 58-59 0.6106993478981977 1101 60-61 0.8682266952356521 1101 62-63 0.7000526089975985 1101 64-65 0.5612487522325686 1101 66-67 0.531885717247782 1101 68-69 0.34573701163967385 1101 70-71 0.3531895770320581 1101 72-73 0.4198025484535961 1101 74-75 0.34197441417491703 1101 76-77 0.7615500336032888 1101 78-79 0.9569730593157537 1101 80-81 0.9663119230160149 1101 82-83 1.0209918001328653 1101 84-85 0.7606630733894733 1101 86-87 0.7125888560058868 1101 88-89 0.5736277589449053 1101 90-91 0.4580961563359125 1101 92-93 0.7506012222994976 1101 94-95 0.6336468453000919 1101 96-97 0.7698384509050555 1101 98-99 0.7905730084025677 1101 100-101 0.4126135470429091 1102 1 0.3875810157362949 1102 2 0.4200666796376673 1102 3 0.10865635133247409 1102 4 0.19002068573536235 1102 5 0.0633680043467919 1102 6 0.08780317692205841 1102 7 0.009949938885526421 1102 8 -0.1815314890436639 1102 9 -0.1162862273188594 1102 10-11 -0.026921994519220505 1102 12-13 -0.05391744160657197 1102 14-15 0.29980390319440886 1102 16-17 0.31215971352937544 1102 18-19 -0.018471173214472003 1102 20-21 -0.031753612085985594 1102 22-23 0.05895127507346132 1102 24-25 -0.07133068717980962 1102 26-27 -0.27802023936772713 1102 28-29 -0.16753437668582905 1102 30-31 -0.1938290397342044 1102 32-33 0.003487792473613638 1102 34-35 0.2387888653751702 1102 36-37 0.2015958583667654 1102 38-39 0.01029732204582956 1102 40-41 0.14766414123781857 1102 42-43 -0.04727880775197235 1102 44-45 -0.42720388618854344 1102 46-47 -0.09770364317355984 1102 48-49 -0.11515677658069734 1102 50-51 -0.13786241294173607 1102 52-53 -0.2100765315009525 1102 54-55 0.04777396013205504 1102 56-57 0.048112845162144424 1102 58-59 0.1735808486713708 1102 60-61 0.20933543821604061 1102 62-63 0.1726095628241353 1102 64-65 0.3084396348727587 1102 66-67 0.17848629102494584 1102 68-69 0.2663810358115626 1102 70-71 0.4772345534298772 1102 72-73 0.35079541693247407 1102 74-75 -0.3383368395892248 1102 76-77 0.23698307936981777 1102 78-79 0.41682137460833957 1102 80-81 0.4898310217925044 1102 82-83 0.6851897329073431 1102 84-85 0.585132448202959 1102 86-87 0.27743890076864375 1102 88-89 0.45260977923735624 1102 90-91 0.5377268127207095 1102 92-93 0.5503387497675547 1102 94-95 0.4488889880797231 1102 96-97 0.3740449632080285 1102 98-99 0.007986907916961883 1102 100-101 0.07934210161277733 1103 1 0.4180734399787198 1103 2 0.44033183115281815 1103 3 0.3341298361809599 1103 4 0.27515326149293884 1103 5 0.10304603464982875 1103 6 0.057689540558421015 1103 7 0.011843878279471198 1103 8 0.11430184428967038 1103 9 0.12036149995387291 1103 10-11 0.20603255093532624 1103 12-13 0.2158742250600909 1103 14-15 0.30259746380047403 1103 16-17 0.14648736504452842 1103 18-19 0.15188867527037786 1103 20-21 0.21062328185341528 1103 22-23 0.13958574477042873 1103 24-25 0.22128294918382352 1103 26-27 0.1751521091171142 1103 28-29 0.4006474414959911 1103 30-31 0.4124683087506469 1103 32-33 0.24917908035240544 1103 34-35 0.34380780476910644 1103 36-37 0.3284897977607031 1103 38-39 0.5317935341670434 1103 40-41 0.47451073214691064 1103 42-43 0.4970393740662047 1103 44-45 0.6757317198720614 1103 46-47 0.7485558265234147 1103 48-49 0.7231007991768763 1103 50-51 0.5585864506946265 1103 52-53 0.6984461957717727 1103 54-55 0.8578591874047774 1103 56-57 0.6896374663742719 1103 58-59 0.7114122880653113 1103 60-61 0.7911536200342191 1103 62-63 0.5043330476726169 1103 64-65 0.5168203166909393 1103 66-67 0.6238461395097943 1103 68-69 0.686693535811564 1103 70-71 0.6831531140359353 1103 72-73 0.5658522351142956 1103 74-75 0.636379069501686 1103 76-77 0.5304963369455713 1103 78-79 0.9266225109719777 1103 80-81 0.788268521792503 1103 82-83 0.9159662480588544 1103 84-85 0.9476324482029632 1103 86-87 0.9737930674353095 1103 88-89 0.9619847792373548 1103 90-91 0.8188821157510127 1103 92-93 0.9585773861311928 1103 94-95 1.2135670183827543 1103 96-97 0.8313839783595469 1103 98-99 0.6996062260987799 1103 100-101 0.5494178591885372 1104 1 0.30172728613256794 1104 2 0.3881375510739211 1104 3 0.20715128588510012 1104 4 0.09918555932330975 1104 5 0.09283277035002158 1104 6 0.2549775090002342 1104 7 0.0798788881414012 1104 8 0.029982317662451408 1104 9 0.07164971494400874 1104 10-11 0.07794453318384598 1104 12-13 -0.029959108273246216 1104 14-15 0.25033846478667243 1104 16-17 0.151557014945908 1104 18-19 0.1564621466707692 1104 20-21 0.0712032867843817 1104 22-23 0.07457403372507088 1104 24-25 0.3186880280792934 1104 26-27 0.39389286355498854 1104 28-29 0.23407939415871226 1104 30-31 0.23160354050606458 1104 32-33 0.36575637325181276 1104 34-35 0.5717294024022408 1104 36-37 0.6342651922380256 1104 38-39 0.576079776770591 1104 40-41 0.4682576502928697 1104 42-43 0.28114317091039 1104 44-45 0.6100759013316264 1104 46-47 0.4459393324405738 1104 48-49 0.2881772291571565 1104 50-51 0.4073259723908791 1104 52-53 0.19671419379938726 1104 54-55 0.46416157892351606 1104 56-57 0.6287369486227945 1104 58-59 0.7967519083808909 1104 60-61 0.5498999218093701 1104 62-63 0.1276688464891862 1104 64-65 0.39978074568502464 1104 66-67 0.4243947095295155 1104 68-69 0.77512733758671 1104 70-71 0.551049440465917 1104 72-73 0.7481747203213942 1104 74-75 0.8195151641762415 1104 76-77 1.187334971067859 1104 78-79 1.1396125257648748 1104 80-81 1.323435558281652 1104 82-83 1.2368303999326251 1104 84-85 1.201287527098426 1104 86-87 1.1639033978100635 1104 88-89 1.1159357161209833 1104 90-91 1.1799891177234016 1104 92-93 1.4859627411607796 1104 94-95 1.6329703714399528 1104 96-97 1.6702738945331177 1104 98-99 1.2351307576569681 1104 100-101 1.162817760569208 1105 1 0.3918846791718025 1105 2 0.3480287571854781 1105 3 0.144567516296231 1105 4 0.14071822306834036 1105 5 -0.03156911904854098 1105 6 -0.06606166021006743 1105 7 -0.04168037724406304 1105 8 -0.25183648424058447 1105 9 -0.18203103126611353 1105 10-11 -0.02203841448254451 1105 12-13 0.19885933553079127 1105 14-15 0.22691452672074064 1105 16-17 -0.023146700757585847 1105 18-19 -0.02245690590157068 1105 20-21 0.05768590193026313 1105 22-23 -0.04080120287606093 1105 24-25 -0.1658898414021479 1105 26-27 -0.008560967251760587 1105 28-29 -0.19229203977202047 1105 30-31 -0.07114870613887803 1105 32-33 -0.06737801618938022 1105 34-35 -0.24090040848737715 1105 36-37 -0.2575002358608103 1105 38-39 -0.2079669125188346 1105 40-41 -0.12213762280025264 1105 42-43 -0.45361204284061074 1105 44-45 -0.402810547178845 1105 46-47 -0.20324982909618683 1105 48-49 -0.14785861484809715 1105 50-51 -0.3912859676531113 1105 52-53 -0.4270431173886493 1105 54-55 -0.5574761271965656 1105 56-57 -0.19977625840959234 1105 58-59 -0.3494411581402588 1105 60-61 -0.23425163933177373 1105 62-63 -0.22641262956080865 1105 64-65 -0.21196660694018377 1105 66-67 0.008989631344569204 1105 68-69 -0.264643820096218 1105 70-71 -0.09362852621382345 1105 72-73 -0.396719810995215 1105 74-75 -0.23278519562799715 1105 76-77 -0.26906988543675325 1105 78-79 -0.1471932311029498 1105 80-81 -0.28316369482613624 1105 82-83 0.013261517030997538 1105 84-85 0.0845770927754863 1105 86-87 -0.3790812721420167 1105 88-89 -0.35697835716994675 1105 90-91 -0.570461063883954 1105 92-93 -0.4599114467218328 1105 94-95 -0.055825392760379344 1105 96-97 -0.3173657214483292 1105 98-99 -0.4475764708272507 1105 100-101 -0.05300168932251026 1106 1 0.44191319479483226 1106 2 0.43193282356379115 1106 3 0.21297725299356784 1106 4 0.34155905542171894 1106 5 0.11067336973447084 1106 6 0.02282380792561156 1106 7 0.07797420519131748 1106 8 0.24889028562066784 1106 9 0.28259697864622524 1106 10-11 -0.03939761543945508 1106 12-13 0.13063633831168886 1106 14-15 0.2163826724986606 1106 16-17 0.0678597745599987 1106 18-19 -0.08981812566366187 1106 20-21 0.10212485073841293 1106 22-23 0.06335526461865015 1106 24-25 0.23760117743834996 1106 26-27 0.189690747470884 1106 28-29 0.25134796689003736 1106 30-31 -0.00475020409232485 1106 32-33 0.1197652230844568 1106 34-35 -0.028773193479580073 1106 36-37 0.026379100154166224 1106 38-39 -0.14727279683120997 1106 40-41 -0.2256707841986838 1106 42-43 -0.2034732499851657 1106 44-45 -0.03393698999366279 1106 46-47 0.1406283965759485 1106 48-49 0.09886335025685611 1106 50-51 -0.14425487300648143 1106 52-53 0.19702507784999312 1106 54-55 -0.11120915030684131 1106 56-57 -0.23536527005305174 1106 58-59 -0.29121377001992244 1106 60-61 -0.0011679649044857854 1106 62-63 -0.6222321887547011 1106 64-65 -0.1361377612425123 1106 66-67 -0.1185155796962789 1106 68-69 0.060136508666204236 1106 70-71 -0.06730204504170345 1106 72-73 0.18115433669047576 1106 74-75 0.05748239699730817 1106 76-77 0.04552935650190548 1106 78-79 -0.026116652775836968 1106 80-81 -0.06865555147077984 1106 82-83 0.09030038407753693 1106 84-85 0.06888719134365218 1106 86-87 0.23091963632030854 1106 88-89 0.16844092342883243 1106 90-91 0.23519487115089532 1106 92-93 0.8287776926110517 1106 94-95 0.8713676605310283 1106 96-97 0.7591432092410386 1106 98-99 0.6995086268576856 1106 100-101 0.33843201563921 1107 1 0.3834021861560899 1107 2 0.29014069965434786 1107 3 0.21917379642560775 1107 4 0.05687153366418585 1107 5 -0.11772040265904593 1107 6 -0.025720245374301953 1107 7 0.049974612224424675 1107 8 -0.05251772757576845 1107 9 -0.264300197293835 1107 10-11 -0.17532257138883267 1107 12-13 -0.059316906438382944 1107 14-15 0.13707280783717835 1107 16-17 0.16484076718520413 1107 18-19 0.07174723796151028 1107 20-21 0.14615940570662644 1107 22-23 0.06969373406707291 1107 24-25 -0.18415474194767256 1107 26-27 -0.3488301156535272 1107 28-29 -0.27442901110339335 1107 30-31 -0.22741605669278186 1107 32-33 -0.15640673769041058 1107 34-35 -0.26939938177523004 1107 36-37 -0.2123645600374573 1107 38-39 -0.064947673784026 1107 40-41 0.026799531841099622 1107 42-43 -0.8222171244047445 1107 44-45 -0.39869488563252276 1107 46-47 0.23117719502494083 1107 48-49 -0.045802106021895383 1107 50-51 0.1102827427435571 1107 52-53 0.05871951381458729 1107 54-55 -0.16101982635668577 1107 56-57 -0.05816929968077744 1107 58-59 0.1661093446402333 1107 60-61 -0.16260211391073298 1107 62-63 -0.5994704691469508 1107 64-65 -0.5934099967647164 1107 66-67 -0.3829103589611549 1107 68-69 -0.15205359721596068 1107 70-71 -0.26954661837996596 1107 72-73 -0.24152544072668647 1107 74-75 0.14601209702462015 1107 76-77 -0.5369778603021338 1107 78-79 -0.489341747437809 1107 80-81 -0.9362010882992422 1107 82-83 -0.7446128803815135 1107 84-85 -0.5984971389530038 1107 86-87 -0.012507582411785734 1107 88-89 0.0719714000324636 1107 90-91 -0.29700091177192434 1107 92-93 -0.38783889827248075 1107 94-95 -0.9051256421677039 1107 96-97 -0.8125428182765404 1107 98-99 -1.0279023748186518 1107 100-101 -1.2329407035025959 1108 1 0.14414486855014985 1108 2 0.019498497819483873 1108 3 0.021183407609530747 1108 4 0.02875445196912807 1108 5 -0.28228134630255397 1108 6 -0.003500935632054336 1108 7 -0.04309659791100984 1108 8 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38.0 6 79.0 7 187.0 8 397.0 9 704.0 10 1189.0 11 1930.0 12 2742.0 13 3783.0 14 4825.0 15 6445.0 16 8697.0 17 11134.0 18 9114.0 19 5427.0 20 4596.0 21 4551.0 22 4877.0 23 5316.0 24 5995.0 25 7006.0 26 8222.0 27 9724.0 28 11969.0 29 14602.0 30 17457.0 31 21213.0 32 26163.0 33 32620.0 34 42686.0 35 56881.0 36 75320.0 37 86416.0 38 49608.0 39 3011.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.38225916478823 18.953167635543956 14.672373650492348 17.992199549175467 2 15.65030355084752 24.32077286174672 37.64324149727281 22.385682090132946 3 23.307518672808353 27.926630085702225 29.745829586537226 19.020021654952192 4 12.194786221882891 17.69230486873789 38.70598901744288 31.406919891936337 5 11.856674655284415 41.224754194024314 31.952941780960174 14.96562936973109 6 27.894528225022114 38.4560191726826 16.544329488275817 17.105123114019474 7 26.922131162582442 36.810889966271645 19.481706310029324 16.785272561116596 8 27.323091264758453 30.013652829108416 19.03707183516778 23.62618407096535 9 26.83305499990824 19.085904094254097 21.403534528637756 32.677506377199904 10-11 25.953910606836338 26.928144514727283 25.05922659517269 22.058718283263694 12-13 30.09226720788932 25.692508702808937 25.489183192981237 18.726040896320505 14-15 23.914860585952805 26.322641971661177 29.58225791208781 20.180239530298206 16-17 19.577955085128327 29.712519864499342 29.061716659754616 21.64780839061772 18-19 19.59098399462695 28.51670453002338 32.643565737271125 19.248745738078547 20-21 22.49186380556445 24.22159075790704 32.40421219525398 20.882333241274523 22-23 20.919077032616553 25.63273253104023 32.85997673129788 20.588213705045344 24-25 22.75388940474399 25.40857559153084 33.00094321964539 18.836591784079772 26-27 21.54724722998044 24.374887602717354 35.45395656785493 18.62390859944728 28-29 19.38765960414136 28.26704493322225 33.22307695130858 19.12221851132781 30-31 20.406777608238684 25.65440510586916 32.82967070777322 21.10914657811894 32-33 20.504411721525678 26.251669994421118 33.21508794080512 20.02883034324808 34-35 22.22499522879751 25.062300894049944 33.351531188983664 19.36117268816889 36-37 21.793489580704918 24.495413601650863 34.53731833559205 19.173778482052168 38-39 20.40217124024208 26.314268307452902 34.10463865879304 19.178921793511968 40-41 20.164428560286648 27.04959925126281 32.503085329430604 20.282886859019943 42-43 19.807476129517728 27.426484420524254 30.907238915883216 21.8588005340748 44-45 21.54465017309896 25.35668112445967 31.375460045015824 21.72320865742555 46-47 23.175369844134657 27.332910645583663 30.476367870470366 19.015351639811314 48-49 22.636767070189475 27.396635515390884 29.901462372537935 20.065135041881703 50-51 19.958839429734336 28.273164519331523 31.76419746063321 20.003798590300935 52-53 21.85464188060852 29.792725671187124 28.01992916521388 20.332703282990476 54-55 20.21097065773475 27.85445869370713 29.2001192961527 22.734451352405426 56-57 22.613637093540415 27.02340873200484 28.812899898789418 21.550054275665325 58-59 24.629632008456117 26.781831094211928 28.000609251446512 20.587927645885443 60-61 21.595293785150023 27.58973493131264 31.287317866787546 19.527653416749786 62-63 20.730889327900034 27.802312852637538 30.564412753263603 20.90238506619882 64-65 20.485692705431898 30.131908492785094 28.954799796993264 20.427599004789748 66-67 21.86441922134645 27.25691162841911 28.10263986126277 22.776029288971674 68-69 21.21365320864717 27.970525276566384 28.68950153365086 22.126319981135588 70-71 23.27705647566179 27.893288067165205 28.19461393769785 20.635041519475156 72-73 22.260075384169326 26.644395183340613 30.46113164336755 20.634397789122513 74-75 20.59742137695388 30.652986923379 28.160152823603248 20.589438876063877 76-77 21.58666541879768 28.004840997634556 27.938960855450894 22.469532728116874 78-79 21.801830737112574 28.41735300190415 28.357337860469382 21.42347840051389 80-81 22.776917662325506 27.890408545152695 28.42232392692312 20.910349865598675 82-83 22.014123088358776 27.24214351590568 29.570571852014993 21.173161543720546 84-85 20.62691673105476 28.641261358151347 29.185057802460392 21.5467641083335 86-87 20.791560408581645 28.88739411280502 27.705715499655415 22.615329978957917 88-89 20.41589567362763 28.7853922518871 26.69572576144741 24.10298631303786 90-91 21.683459470572362 26.897794877537045 27.71317438144415 23.70557127044644 92-93 22.64022484054984 28.453010031756985 27.838699110602576 21.068066017090597 94-95 21.381522802393935 28.291522673924575 28.034859243462286 22.292095280219204 96-97 19.227631495632338 28.447031466083274 30.048921137951 22.27641590033339 98-99 20.799322396609046 29.63119985537609 26.534816646065103 23.03466110194976 100-101 19.71412341550572 28.162037109262084 27.52681698924751 24.597022485984688 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1040.0 1 778.0 2 970.0 3 1204.5 4 764.5 5 591.0 6 902.5 7 1696.5 8 2517.5 9 2804.5 10 2390.5 11 1806.0 12 1651.0 13 1726.5 14 1786.0 15 1792.0 16 1821.0 17 1867.5 18 1956.0 19 2057.0 20 2105.0 21 2306.0 22 2631.5 23 2884.5 24 3164.5 25 3559.0 26 4152.5 27 4734.5 28 5229.0 29 5986.0 30 6907.0 31 7677.0 32 8660.0 33 9642.0 34 10306.0 35 10710.5 36 11388.5 37 12422.5 38 12686.0 39 12323.0 40 12187.5 41 12757.5 42 13460.0 43 14140.0 44 14933.0 45 15625.5 46 16326.0 47 24900.0 48 43356.5 49 41063.5 50 23151.0 51 15542.0 52 12814.5 53 12320.5 54 12180.5 55 11580.5 56 10673.0 57 9883.5 58 9677.0 59 9090.5 60 7997.5 61 6941.5 62 5900.0 63 4523.5 64 3103.0 65 2292.5 66 1668.0 67 1322.0 68 997.0 69 653.0 70 514.0 71 430.0 72 371.5 73 358.0 74 297.5 75 235.0 76 185.5 77 116.0 78 73.5 79 65.0 80 51.0 81 35.5 82 28.0 83 17.5 84 12.0 85 9.0 86 4.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0067126409783058 2 0.010643334520003964 3 0.0058721845627608075 4 0.014313449871729467 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0058721845627608075 10-11 1.8350576758627523E-4 12-13 5.505173027588256E-4 14-15 9.175288379313762E-5 16-17 0.0 18-19 0.0 20-21 9.175288379313762E-5 22-23 7.340230703451009E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 9.175288379313762E-5 32-33 0.005505173027588257 34-35 0.0011010346055176513 36-37 0.0028443393975872665 38-39 0.0 40-41 0.004495891305863743 42-43 0.013671179685177505 44-45 0.0041288797706911925 46-47 0.0030278451651735414 48-49 0.013579426801384369 50-51 5.505173027588256E-4 52-53 0.0022020692110353026 54-55 0.014588708523108881 56-57 0.007064972052071596 58-59 0.0020185634434490276 60-61 0.008349512425175523 62-63 0.005596925911381395 64-65 0.024314514205181468 66-67 0.004954655724829431 68-69 9.175288379313762E-5 70-71 0.0031195980489666787 72-73 0.0 74-75 0.0 76-77 0.002385574978621578 78-79 0.014588708523108881 80-81 0.021745433458973618 82-83 0.016423766198971634 84-85 0.012661897963452991 86-87 0.01495572005828143 88-89 0.034866095841392296 90-91 0.030278451651735414 92-93 0.0041288797706911925 94-95 0.01192787489310789 96-97 0.015689743128626532 98-99 0.014772214290695158 100-101 0.016790777734144183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 544942.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.25264734917213 #Duplication Level Percentage of deduplicated Percentage of total 1 82.81071592916575 43.2707913617917 2 9.937219753429044 10.384920788143102 3 2.8991735559223537 4.544684802649685 4 1.3391384557070296 2.798941179110975 5 0.725437935258128 1.8953026302377267 6 0.42289157841831027 1.3258322708416042 7 0.3062239573268652 1.1200708716448038 8 0.22052483339671988 0.9218405080970995 9 0.16833313104466027 0.7916266560292753 >10 1.031361311257049 10.072915817430745 >50 0.08177332651570847 2.9358120386768065 >100 0.04527364417197321 4.580226205576383 >500 0.002807667855626246 1.0414439710709595 >1k 0.008072045084925455 9.361809248665693 >5k 7.019169639065615E-4 2.192919000892152 >10k+ 3.5095848195328073E-4 2.7608626491412736 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15055 2.7626793310113738 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6516 1.1957235815921694 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5442 0.9986383872045099 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4623 0.8483471635513504 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3932 0.7215446781492343 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3738 0.6859445592374969 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3714 0.6815404208154263 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3425 0.6285072539829927 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3061 0.5617111545815885 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3049 0.5595090853705532 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2963 0.5437275893581335 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2704 0.49619959555328824 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2171 0.39839102142980354 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2082 0.382059008114625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1751 0.32131859904356797 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1666 0.30572060879873453 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1458 0.2675514091407893 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.25488951117733627 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1301 0.23874100362974407 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1235 0.22662962296904993 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1222 0.22424404799042835 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1182 0.21690381728697736 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1182 0.21690381728697736 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1091 0.20020479243662626 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1078 0.1978192174580047 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1033 0.18956145791662232 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 858 0.15744794858902417 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.15377783323729863 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 780 0.1431344987172947 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 759 0.13928087759798288 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 710 0.1302890949862554 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 672 0.12331587581797697 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 546 0.10019414910210626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.175288379313762E-5 0.0 22-23 0.0 0.0 0.0 5.505173027588258E-4 0.0 24-25 0.0 0.0 0.0 0.0014680461406902019 0.0 26-27 0.0 0.0 0.0 0.0024773278624147157 0.0 28-29 0.0 0.0 0.0 0.004587644189656881 0.0 30-31 0.0 0.0 0.0 0.009267041263106901 0.0 32-33 0.0 0.0 0.0 0.01422169698793633 0.0 34-35 0.0 0.0 0.0 0.019910375783110862 0.0 36-37 0.0 0.0 0.0 0.02660833630000991 0.0 38-39 0.0 0.0 0.0 0.03945374003104918 0.0 40-41 0.0 0.0 0.0 0.061933196560367894 0.0 42-43 0.0 0.0 0.0 0.09450547030693174 0.0 44-45 0.0 0.0 0.0 0.13203239977832504 0.0 46-47 0.0 0.0 0.0 0.20791203467524982 0.0 48-49 0.0 0.0 0.0 0.28085557729079424 0.0 50-51 0.0 0.0 0.0 0.3367330835208151 0.0 52-53 0.0 0.0 0.0 0.3970147281729065 1.8350576758627523E-4 54-55 0.0 0.0 0.0 0.45518605649775573 1.8350576758627523E-4 56-57 0.0 0.0 0.0 0.5750153227315935 1.8350576758627523E-4 58-59 0.0 0.0 0.0 0.7037446186933656 1.8350576758627523E-4 60-61 0.0 0.0 0.0 0.8402729097775543 1.8350576758627523E-4 62-63 0.0 0.0 0.0 0.9770764595131225 1.8350576758627523E-4 64-65 0.0 0.0 0.0 1.1258996370255918 1.8350576758627523E-4 66-67 0.0 0.0 0.0 1.3448220177560182 1.8350576758627523E-4 68-69 0.0 0.0 0.0 1.6113641451750829 1.8350576758627523E-4 70-71 0.0 0.0 0.0 1.8191844269665396 1.8350576758627523E-4 72-73 0.0 0.0 0.0 1.9965427513386746 1.8350576758627523E-4 74-75 0.0 0.0 0.0 2.2021609639190958 1.8350576758627523E-4 76-77 0.0 0.0 0.0 2.4272307878636625 1.8350576758627523E-4 78-79 0.0 0.0 0.0 2.6897357883958293 1.8350576758627523E-4 80-81 0.0 0.0 0.0 3.054728760124931 1.8350576758627523E-4 82-83 0.0 0.0 0.0 3.418437191480928 1.8350576758627523E-4 84-85 0.0 0.0 0.0 3.8554561769876425 1.8350576758627523E-4 86-87 0.0 0.0 0.0 4.335232006341959 1.8350576758627523E-4 88-89 0.0 0.0 0.0 4.9092380473518284 1.8350576758627523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGTAAG 115 1.0345684E-7 33.043926 2 GTAAGCG 90 2.1100277E-10 29.025505 94-95 GTACATG 5425 0.0 25.383297 1 TACATGG 5575 0.0 24.368042 2 CATGGGG 1220 0.0 23.745964 4 ATGGGAG 1390 0.0 23.575129 5 ACATGGG 5400 0.0 23.482117 3 GTGTAGG 185 2.30164E-7 23.341927 1 GTATAGG 165 2.2226905E-6 23.263332 1 GAGTACT 5185 0.0 22.761318 12-13 AGTACTT 5195 0.0 21.849028 12-13 CATGGGA 4095 0.0 21.571424 4 ATGGGAC 600 0.0 21.371367 5 TACCTGG 445 0.0 21.348602 2 ATGGGGG 585 0.0 21.107521 5 GTACTTT 5610 0.0 20.994646 14-15 CTAGACC 115 0.003724468 20.648664 3 CTGGACG 115 0.003724468 20.648664 4 TTGCGTA 70 4.8540765E-4 20.35555 60-61 ACGCAGT 140 4.8527957E-4 20.353682 5 >>END_MODULE