##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 582958 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80553659097225 31.0 31.0 34.0 30.0 34.0 2 31.88236030726056 31.0 31.0 34.0 30.0 34.0 3 31.96959300670031 31.0 31.0 34.0 30.0 34.0 4 35.73129796657735 37.0 35.0 37.0 35.0 37.0 5 35.531480827092174 37.0 35.0 37.0 33.0 37.0 6 35.69687867736612 37.0 35.0 37.0 33.0 37.0 7 35.650635208711435 37.0 35.0 37.0 33.0 37.0 8 35.639389115510895 37.0 35.0 37.0 33.0 37.0 9 37.208989326846876 39.0 37.0 39.0 33.0 39.0 10-11 37.07313048281351 39.0 37.0 39.0 33.0 39.0 12-13 36.979554273206645 39.0 37.0 39.0 33.0 39.0 14-15 37.85058614857331 39.5 37.0 41.0 33.0 41.0 16-17 37.87422678820772 39.0 37.0 41.0 33.0 41.0 18-19 38.16374249945965 40.0 37.0 41.0 33.0 41.0 20-21 38.21625571653533 40.0 37.5 41.0 33.0 41.0 22-23 38.093532467176026 40.0 37.0 41.0 33.0 41.0 24-25 37.90611330490361 40.0 37.0 41.0 33.0 41.0 26-27 37.60252539634074 39.5 36.5 41.0 32.5 41.0 28-29 37.50330812854442 39.0 36.0 41.0 32.0 41.0 30-31 37.396037964999195 39.0 36.0 41.0 32.0 41.0 32-33 37.09788441019765 39.0 35.0 41.0 32.0 41.0 34-35 36.7750661282631 39.0 35.0 41.0 31.0 41.0 36-37 36.43655374829748 38.5 35.0 40.5 30.5 41.0 38-39 36.20625756915592 38.0 35.0 40.0 30.0 41.0 40-41 35.9835442690554 38.0 35.0 40.0 30.0 41.0 42-43 35.60213686063147 38.0 35.0 40.0 29.0 41.0 44-45 35.125800658023394 38.0 35.0 40.0 26.5 41.0 46-47 34.48555384779006 38.0 34.0 40.0 23.5 41.0 48-49 33.846999269244094 38.0 33.0 40.0 20.5 41.0 50-51 33.41393119229859 38.0 33.0 40.0 16.5 41.0 52-53 33.16049269415635 38.0 33.0 40.0 14.0 41.0 54-55 32.9145049900679 37.0 33.0 40.0 10.5 41.0 56-57 32.512922886382896 37.0 31.5 40.0 8.0 41.0 58-59 32.14957338264506 36.0 31.0 39.0 6.5 41.0 60-61 31.65324946222541 36.0 31.0 39.0 2.0 40.0 62-63 31.33733304972228 35.0 31.0 39.0 2.0 40.0 64-65 30.95864882204207 35.0 30.0 38.5 2.0 40.0 66-67 30.42595778769654 35.0 29.0 38.0 2.0 40.0 68-69 30.071629517049253 34.5 29.0 37.0 2.0 39.5 70-71 29.554575972883125 34.0 28.5 37.0 2.0 39.0 72-73 29.069521303421517 34.0 28.0 36.0 2.0 39.0 74-75 28.58321868813877 34.0 27.0 35.5 2.0 38.5 76-77 27.219655618415047 31.5 26.0 34.5 2.0 36.0 78-79 27.97761245235506 33.0 27.0 35.0 2.0 37.0 80-81 27.6957919781528 33.0 26.0 35.0 2.0 36.5 82-83 27.321896260107934 33.0 26.0 35.0 2.0 36.0 84-85 26.882320853303327 33.0 25.0 35.0 2.0 36.0 86-87 26.59713478501024 33.0 25.0 35.0 2.0 35.0 88-89 26.151170410218235 32.5 24.0 35.0 2.0 35.0 90-91 25.56877253592883 32.0 21.5 34.0 2.0 35.0 92-93 25.04859097910999 32.0 19.5 34.0 2.0 35.0 94-95 24.38813688121614 31.0 14.5 34.0 2.0 35.0 96-97 23.586603151513486 31.0 2.0 34.0 2.0 35.0 98-99 22.69548921191578 31.0 2.0 34.0 2.0 35.0 100-101 19.780063057715992 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23065326839992295 1101 2 0.28103274533067335 1101 3 0.25218672225323857 1101 4 0.16668519073846255 1101 5 -0.07987344536287111 1101 6 0.12440955481215354 1101 7 0.1703115896203613 1101 8 0.04452564513699997 1101 9 0.03439885675488341 1101 10-11 0.18882425469920605 1101 12-13 0.15245428824728435 1101 14-15 0.22210262135877912 1101 16-17 0.34799538599443736 1101 18-19 0.3701532093469311 1101 20-21 0.29055063582571705 1101 22-23 0.20586762276277426 1101 24-25 0.04872031818000977 1101 26-27 0.14645539748706682 1101 28-29 0.09643269666140242 1101 30-31 0.172049211024337 1101 32-33 0.372428811254089 1101 34-35 0.23701272225876124 1101 36-37 0.030760452242489578 1101 38-39 0.41377407216043594 1101 40-41 0.42740680578619816 1101 42-43 0.5789942455056618 1101 44-45 0.5310997824190338 1101 46-47 0.7747991659877016 1101 48-49 0.9035035380404324 1101 50-51 0.7342411426706477 1101 52-53 0.8294023352005055 1101 54-55 0.7545738896976388 1101 56-57 0.8235374166609049 1101 58-59 0.782892135904266 1101 60-61 0.9006463301778957 1101 62-63 1.1763649452457123 1101 64-65 1.0211026707642255 1101 66-67 0.8326328031473089 1101 68-69 0.9868958629178479 1101 70-71 1.0578735117837468 1101 72-73 1.1736042455891855 1101 74-75 1.0743141851217857 1101 76-77 0.9433340380810016 1101 78-79 0.6412904545720899 1101 80-81 0.8890022130700359 1101 82-83 1.0515201047773992 1101 84-85 0.9386583423046844 1101 86-87 1.1313734160538225 1101 88-89 0.9086984653559789 1101 90-91 0.7362710021153198 1101 92-93 0.6447851716054913 1101 94-95 0.667965074031013 1101 96-97 0.9093058967425058 1101 98-99 1.296767894381773 1101 100-101 1.5526188605141016 1102 1 0.2576236525131641 1102 2 0.22548423740120782 1102 3 0.2609900881994598 1102 4 0.21528562029127585 1102 5 0.06798398820885154 1102 6 0.07296717003643494 1102 7 0.18033331392779672 1102 8 0.2531899966584916 1102 9 0.2888771826617784 1102 10-11 0.242236010491915 1102 12-13 0.21836544939653635 1102 14-15 0.4718801806601576 1102 16-17 0.35376254652349814 1102 18-19 0.3127806876886936 1102 20-21 0.1694072289680264 1102 22-23 0.16901558591073496 1102 24-25 0.28687590157368703 1102 26-27 0.12642736555427092 1102 28-29 0.18809896689909777 1102 30-31 0.46951937754212025 1102 32-33 0.4437538706743922 1102 34-35 0.7855884851202362 1102 36-37 0.5010586725407151 1102 38-39 0.44117788527853463 1102 40-41 0.35277596925060095 1102 42-43 0.41553028442265116 1102 44-45 0.5002065134305269 1102 46-47 0.8766655535683725 1102 48-49 0.701707538625385 1102 50-51 0.859744387221518 1102 52-53 1.0282621173936235 1102 54-55 1.0127649931268365 1102 56-57 0.9156142111186512 1102 58-59 0.6463923922616708 1102 60-61 0.8284958175511896 1102 62-63 0.6154575986240793 1102 64-65 0.7077625717098392 1102 66-67 0.8359896755517973 1102 68-69 0.8758556685443217 1102 70-71 0.985989927995405 1102 72-73 0.8870631852385991 1102 74-75 0.9857607632350316 1102 76-77 0.6326685131297616 1102 78-79 0.7188557464230989 1102 80-81 1.0858855766200648 1102 82-83 1.1574591392878624 1102 84-85 0.8171740065346356 1102 86-87 0.8322844979172892 1102 88-89 0.795666876133911 1102 90-91 1.0178007598355556 1102 92-93 0.8759023789131746 1102 94-95 0.8370437907287815 1102 96-97 1.1872603056492963 1102 98-99 1.3116863973955155 1102 100-101 1.4150174848174863 1103 1 0.03540143029094267 1103 2 0.2505712902660413 1103 3 0.2211831872814436 1103 4 0.14049929550495222 1103 5 -0.1908795762102713 1103 6 -0.01772608732348857 1103 7 0.04753812557705572 1103 8 0.08224982571832129 1103 9 0.06815860083208491 1103 10-11 0.11023221182144738 1103 12-13 0.0036218596529451474 1103 14-15 -0.04363105707330561 1103 16-17 0.37528834582707304 1103 18-19 0.19668382159182585 1103 20-21 0.19255537711617166 1103 22-23 0.2352153326660371 1103 24-25 0.29328615798394253 1103 26-27 0.469336514018984 1103 28-29 0.45392676050467884 1103 30-31 0.24009550922936995 1103 32-33 0.27506916032300666 1103 34-35 0.4095201090518614 1103 36-37 0.37760188241725956 1103 38-39 0.594113940992365 1103 40-41 0.6322156653569593 1103 42-43 0.3932526649228123 1103 44-45 0.38553414875816117 1103 46-47 0.35169720915558855 1103 48-49 0.3796119387519994 1103 50-51 0.7113983916532973 1103 52-53 0.7771937413252488 1103 54-55 0.4754905391857136 1103 56-57 0.5077319699030767 1103 58-59 0.5120144245503688 1103 60-61 0.48388518127388735 1103 62-63 0.5617618088171774 1103 64-65 0.5135555441694812 1103 66-67 0.6866940123672478 1103 68-69 0.5047730474617005 1103 70-71 0.7099927769982521 1103 72-73 0.8924050655804798 1103 74-75 0.9181760845392439 1103 76-77 0.7033396115786381 1103 78-79 0.4767300737418658 1103 80-81 0.6054724712069586 1103 82-83 0.7131334032954619 1103 84-85 0.7191999641161466 1103 86-87 0.5080521459071576 1103 88-89 0.5504943531836126 1103 90-91 0.8216943970625259 1103 92-93 0.7285851899293192 1103 94-95 1.0303407201368202 1103 96-97 1.0525657820658836 1103 98-99 0.8501083663730391 1103 100-101 1.2036768706990948 1104 1 0.18303813488967435 1104 2 0.21498215938858323 1104 3 0.23301150325884734 1104 4 0.1916258919901921 1104 5 0.16988583694614334 1104 6 0.1099430087000357 1104 7 0.16812751691187344 1104 8 0.024328843746708628 1104 9 0.11397660269791032 1104 10-11 0.04400027217178604 1104 12-13 0.05010817143462276 1104 14-15 0.055349844298596906 1104 16-17 0.22047959256543948 1104 18-19 0.11009003563095376 1104 20-21 0.021557422890374767 1104 22-23 0.18852420668518022 1104 24-25 0.039954671530090025 1104 26-27 0.09505336371613993 1104 28-29 0.16147931369717128 1104 30-31 -0.14505818429282158 1104 32-33 -0.0432671564310283 1104 34-35 0.12287730975083377 1104 36-37 0.19404337185453358 1104 38-39 0.30491096420148267 1104 40-41 0.1609381930246414 1104 42-43 0.08006866837032334 1104 44-45 0.4760987256096598 1104 46-47 0.24418692108974227 1104 48-49 0.20780005526642498 1104 50-51 0.2730847583677942 1104 52-53 0.18231208367988216 1104 54-55 0.228218238115943 1104 56-57 0.15578067069439783 1104 58-59 0.1239069077508681 1104 60-61 0.1675200531998975 1104 62-63 0.3546017175150311 1104 64-65 0.40672066940211593 1104 66-67 0.2863621660086757 1104 68-69 0.44888008123118084 1104 70-71 0.6624800264264294 1104 72-73 0.3721728560022832 1104 74-75 0.417648066430349 1104 76-77 0.38099402727806364 1104 78-79 0.3509262076074826 1104 80-81 0.644468266004445 1104 82-83 0.7057085926006117 1104 84-85 0.587316194398344 1104 86-87 0.3158571059624684 1104 88-89 0.5349284740453015 1104 90-91 0.4024953744879767 1104 92-93 0.5017049245469423 1104 94-95 0.569200366767209 1104 96-97 0.8954910497450221 1104 98-99 1.0708631481334443 1104 100-101 1.399804224878487 1105 1 -0.022184230235893665 1105 2 -0.011884421536077383 1105 3 -0.004279500599540853 1105 4 0.037442584605102525 1105 5 -0.034473467189783946 1105 6 0.04094889782208355 1105 7 0.19958693612259992 1105 8 0.04659920183240729 1105 9 -0.04936192028320363 1105 10-11 -0.028953765599830206 1105 12-13 -0.08693237550912869 1105 14-15 0.04350390914662938 1105 16-17 0.07601841338721016 1105 18-19 0.028234623730860164 1105 20-21 0.22003606636156547 1105 22-23 0.1992786235267161 1105 24-25 0.1327675734457543 1105 26-27 0.32081150356587074 1105 28-29 0.29459377927626207 1105 30-31 0.26872879194761623 1105 32-33 0.18123869884549038 1105 34-35 0.2419374194103483 1105 36-37 0.047280696703921876 1105 38-39 0.38115647966888844 1105 40-41 0.3064993518955532 1105 42-43 0.2445240938086144 1105 44-45 0.5014151813058589 1105 46-47 0.49285002269402156 1105 48-49 0.39924729008669857 1105 50-51 0.40702843940752587 1105 52-53 0.5975459562853089 1105 54-55 0.391810602564604 1105 56-57 0.4479098929241374 1105 58-59 0.6343736375958606 1105 60-61 0.5989574728167675 1105 62-63 0.4713011015264037 1105 64-65 0.48687534346967 1105 66-67 0.591268075438041 1105 68-69 0.6951655344228129 1105 70-71 0.6258507026600988 1105 72-73 0.7059490016734351 1105 74-75 0.32717896739833563 1105 76-77 0.22899375651383025 1105 78-79 0.1677965115403346 1105 80-81 0.3442161168133495 1105 82-83 0.5161059391111351 1105 84-85 0.5754448750996204 1105 86-87 0.4985975784460557 1105 88-89 0.4859793777209269 1105 90-91 0.38406648394441945 1105 92-93 0.3651551377737796 1105 94-95 0.4718775481115536 1105 96-97 0.5881228780804939 1105 98-99 0.7754593709724098 1105 100-101 0.8852337246415694 1106 1 0.003141254921228409 1106 2 0.03345674737607496 1106 3 -0.04645557436987957 1106 4 9.778541578313593E-4 1106 5 -0.0913034590644628 1106 6 -0.05109868253354222 1106 7 0.15964532504192874 1106 8 0.09579517318593389 1106 9 -0.0747582657793231 1106 10-11 -0.005295860420410747 1106 12-13 0.00496876014590697 1106 14-15 -0.10103701033713008 1106 16-17 -0.12474073704456856 1106 18-19 -0.054199570634324346 1106 20-21 0.01003672772473152 1106 22-23 -0.1989210514605304 1106 24-25 -0.3076932077186214 1106 26-27 -0.28798509891708335 1106 28-29 -0.20875555274693625 1106 30-31 -0.04064508609701534 1106 32-33 -0.4394152003627667 1106 34-35 -0.17348604197548667 1106 36-37 -0.5735716114535307 1106 38-39 -0.16245333496046044 1106 40-41 -0.12362999184323087 1106 42-43 -0.5457623709218282 1106 44-45 -0.7283471058769209 1106 46-47 -1.2319614888042523 1106 48-49 -1.2949761750783537 1106 50-51 -1.0909307828293962 1106 52-53 -1.3785504893600873 1106 54-55 -1.220753407941622 1106 56-57 -1.1940304521184757 1106 58-59 -1.2363605699965952 1106 60-61 -1.276699166324594 1106 62-63 -0.972775918938062 1106 64-65 -0.8252526397505981 1106 66-67 -0.7650484151800505 1106 68-69 -0.5914621748041462 1106 70-71 -0.912269797708376 1106 72-73 -0.9657668578152361 1106 74-75 -0.5528828228172706 1106 76-77 -0.9826708000894051 1106 78-79 -0.8530387901357521 1106 80-81 -0.8112868971130673 1106 82-83 -0.5291832655524225 1106 84-85 -0.5793684315272394 1106 86-87 -0.4534748502684458 1106 88-89 -0.5482201838897787 1106 90-91 -0.6630698135556266 1106 92-93 -0.4854193033743499 1106 94-95 -0.2164219927134461 1106 96-97 -0.5717380466941684 1106 98-99 -0.37866659036619055 1106 100-101 -0.5155334566286243 1107 1 -0.14012191914545014 1107 2 0.04871321500952064 1107 3 0.004186680167318713 1107 4 -0.035449968188487446 1107 5 -0.025284502035063383 1107 6 0.074376412151409 1107 7 0.16543663740541348 1107 8 -0.14396315474796495 1107 9 0.013767988838026213 1107 10-11 -0.07082218851461874 1107 12-13 -0.17578431085278368 1107 14-15 -0.03327297792716166 1107 16-17 -0.206864485433087 1107 18-19 -0.05993243549702498 1107 20-21 -0.1268890673282712 1107 22-23 -0.25617789315797523 1107 24-25 -0.21732669179084496 1107 26-27 -0.0503041621539424 1107 28-29 0.1031620596559577 1107 30-31 0.16211430391146564 1107 32-33 0.14557080563562153 1107 34-35 -0.07497194443830324 1107 36-37 0.0839544227332567 1107 38-39 -0.09011536813192578 1107 40-41 -0.10309320999602534 1107 42-43 -0.07374870422621882 1107 44-45 -0.18933924385309808 1107 46-47 -0.18681496926942742 1107 48-49 0.11362362319427177 1107 50-51 0.49220327235840955 1107 52-53 0.3209141315327244 1107 54-55 0.19843039775884108 1107 56-57 -0.023470625256670985 1107 58-59 0.14897211317787296 1107 60-61 0.31985276511481686 1107 62-63 0.30442888778440036 1107 64-65 0.34704057299162017 1107 66-67 0.44938878548525096 1107 68-69 0.42712246051684843 1107 70-71 0.14288794374660085 1107 72-73 0.005473631941423918 1107 74-75 0.2041843149919167 1107 76-77 0.17537124203287036 1107 78-79 0.5573027436602622 1107 80-81 0.49529195357527556 1107 82-83 0.5422980279074707 1107 84-85 0.370852667018589 1107 86-87 0.12158844002525981 1107 88-89 0.46293493911716155 1107 90-91 0.48179148217536394 1107 92-93 0.653568853492061 1107 94-95 0.6483355002561524 1107 96-97 0.4403539062539217 1107 98-99 0.39305083099687366 1107 100-101 0.6250995799027308 1108 1 0.05688291177242277 1108 2 0.15629145487509533 1108 3 -0.0030554958461266324 1108 4 -0.15147601313702808 1108 5 -0.312072991848126 1108 6 0.015607246009842868 1108 7 -0.08513677154228816 1108 8 -0.015527952059457562 1108 9 -0.08422823044363525 1108 10-11 -0.08310112151188775 1108 12-13 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-1.4021507664758879 2316 84-85 -1.447892835455285 2316 86-87 -1.7356167366506163 2316 88-89 -1.5484780577546111 2316 90-91 -1.7051154502601413 2316 92-93 -1.1899899433894028 2316 94-95 -1.2938277119375954 2316 96-97 -1.3606979089755846 2316 98-99 -1.577713850979091 2316 100-101 -1.3201713529354535 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 13.0 5 25.0 6 77.0 7 193.0 8 391.0 9 740.0 10 1281.0 11 2054.0 12 2934.0 13 3997.0 14 4895.0 15 6825.0 16 8983.0 17 11921.0 18 9971.0 19 5860.0 20 4905.0 21 4859.0 22 5314.0 23 5494.0 24 6441.0 25 7572.0 26 8893.0 27 10839.0 28 12868.0 29 15630.0 30 18781.0 31 22820.0 32 28067.0 33 35402.0 34 45744.0 35 61873.0 36 80954.0 37 92693.0 38 50882.0 39 2763.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.45991488777541 18.991351499759766 14.610989086416362 17.937744526048462 2 15.77716745206551 24.411808601704145 37.4871125399061 22.32391140632425 3 23.257098549411943 27.808370044052865 29.914261599901188 19.020269806634005 4 12.188377530418196 17.48928308605952 38.67163444302067 31.65070494050162 5 11.837661012122762 41.2957819927164 31.953176328108785 14.913380667052062 6 27.861794029051936 38.46087869410385 16.62852771049616 17.04879956634806 7 27.00623372524264 36.62939697199455 19.519416493126435 16.84495280963637 8 27.362005496107784 30.01622758414843 19.032074351840098 23.589692567903693 9 26.867530435767108 18.99565828228541 21.43720977306763 32.69960150887985 10-11 25.923568845676176 26.890108344369047 25.19203850719437 21.994284302760413 12-13 30.0631098272242 25.57860681496933 25.574404067573315 18.78387929023316 14-15 23.95965653677151 26.232685958954928 29.5757143261494 20.231943178124162 16-17 19.676994915358907 29.568285837799262 29.088126917033204 21.666592329808623 18-19 19.56161507347013 28.643744489311405 32.51597885267892 19.278661584539538 20-21 22.573581630237513 24.189735795717702 32.31733675496348 20.919345819081308 22-23 20.95618917247549 25.619286061657142 32.74245882362023 20.682065942247142 24-25 22.787659007653296 25.392678464675022 32.856678471536654 18.962984056135028 26-27 21.576459891580317 24.3446785150621 35.35588073835175 18.722980855005833 28-29 19.43778262689476 28.266292003760352 33.12052686113456 19.175398508210336 30-31 20.530526684152473 25.597436482436713 32.72927820443984 21.142758628970974 32-33 20.60404215755563 26.160979559349247 33.16939871962892 20.065579563466194 34-35 22.265835423450252 25.024017759417532 33.28063722546843 19.42950959166378 36-37 21.821990462947632 24.46179303103788 34.44464555156296 19.271570954451526 38-39 20.426882094901192 26.313988138637605 34.04874927303264 19.21038049342856 40-41 20.31838065014152 27.043914572433316 32.33356205506476 20.304142722360407 42-43 19.884261780742374 27.393865891533377 30.856320444083448 21.865551883640805 44-45 21.54969889981139 25.267456391721012 31.283799257391987 21.899045451075615 46-47 23.23072133169456 27.270473868976502 30.43040089612175 19.068403903207187 48-49 22.670800794232807 27.377702299930096 29.851574527941814 20.099922377895282 50-51 20.057432174540292 28.099995111060412 31.756921923634764 20.085650790764536 52-53 21.85775429507578 29.603386312367586 28.081518522648324 20.457340869908307 54-55 20.19563224089321 27.81643780862384 29.228269922400695 22.759660028082248 56-57 22.663489678218415 26.947792404733235 28.841977417993558 21.546740499054795 58-59 24.54741244370263 26.607010679239423 28.152416959500197 20.693159917557747 60-61 21.660390952679116 27.474418245601996 31.27955947060993 19.585631331108956 62-63 20.780262357685153 27.753936523267452 30.569310858602538 20.896490260444857 64-65 20.56866687711882 29.99406387680314 28.977374106150233 20.459895139927806 66-67 21.929600345839717 27.189331805417126 28.14124876271381 22.739819086029346 68-69 21.3238296193368 27.87127099308665 28.708335477673135 22.09656390990342 70-71 23.257850479794428 27.849948023699824 28.190967445339115 20.701234051166637 72-73 22.356501169055957 26.546490344781446 30.453950517112133 20.643057969050467 74-75 20.655722517966236 30.560869035630528 28.163970087442248 20.619438358960988 76-77 21.61448616768609 27.89964807004633 27.988852835296267 22.49701292697132 78-79 21.80816024044301 28.32547639143353 28.369736020476083 21.49662734764738 80-81 22.776500660479318 27.85344221234839 28.29090254070097 21.079154586471326 82-83 22.0170060995851 27.323585090970536 29.404100300475978 21.255308508968387 84-85 20.607855701177645 28.686750677430062 29.09599649340412 21.609397127988174 86-87 20.832532766074248 28.86022095656351 27.759040691690114 22.548205585672136 88-89 20.510390002539168 28.73455053288223 26.720629713760236 24.03442975081837 90-91 21.639592619662825 26.766075429873414 27.78178112228616 23.8125508281776 92-93 22.658320567736006 28.437490886821614 27.85639174744874 21.04779679799364 94-95 21.45377630858909 28.16144797808339 28.04762778825156 22.33714792507595 96-97 19.277550722835247 28.391493230522475 29.948322962187845 22.382633084454433 98-99 20.9237974336101 29.52677897481644 26.522078501338093 23.027345090235364 100-101 19.88116590197345 28.039408934601788 27.463766064776333 24.615659098648425 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1132.0 1 835.5 2 986.5 3 1218.5 4 813.0 5 643.5 6 970.0 7 1788.5 8 2618.0 9 2966.5 10 2487.5 11 1855.0 12 1711.0 13 1753.0 14 1855.0 15 1932.0 16 1941.0 17 1959.0 18 2088.0 19 2152.5 20 2209.0 21 2441.5 22 2772.5 23 3079.0 24 3330.0 25 3693.5 26 4292.0 27 4971.5 28 5631.0 29 6407.5 30 7317.5 31 8165.0 32 9214.5 33 10115.0 34 10916.5 35 11502.0 36 12298.0 37 13358.5 38 13577.0 39 13332.5 40 13166.0 41 13659.0 42 14403.5 43 15117.0 44 15890.0 45 16636.5 46 17456.0 47 26397.5 48 46216.5 49 44018.0 50 24753.5 51 16783.0 52 14038.0 53 13377.0 54 13011.0 55 12482.0 56 11767.0 57 10799.5 58 10198.5 59 9685.5 60 8595.5 61 7366.0 62 6490.5 63 5058.5 64 3381.0 65 2511.0 66 1862.5 67 1398.5 68 1068.5 69 699.5 70 514.0 71 437.5 72 380.0 73 359.0 74 297.5 75 250.0 76 212.0 77 134.5 78 86.0 79 73.5 80 50.5 81 34.5 82 30.0 83 24.0 84 14.0 85 5.0 86 1.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033964711008340226 2 0.004631551501137303 3 0.0037738567787044693 4 1.7153894448656678E-4 5 8.576947224328339E-4 6 3.4307788897313356E-4 7 0.0 8 0.0 9 0.001543850500379101 10-11 3.4307788897313356E-4 12-13 0.0010292336669194008 14-15 0.0023157757505686515 16-17 0.004460012556650737 18-19 0.0 20-21 0.0 22-23 0.0014580810281358176 24-25 0.0011150031391626842 26-27 0.006518479890489538 28-29 0.0058323241125432706 30-31 0.001543850500379101 32-33 3.4307788897313356E-4 34-35 0.00960618089124774 36-37 0.012608112419762658 38-39 0.009777719835734307 40-41 0.0013723115558925342 42-43 7.719252501895505E-4 44-45 0.0021442368060820846 46-47 2.573084167298502E-4 48-49 9.434641946761173E-4 50-51 0.0016296199726223844 52-53 0.003945395723191036 54-55 0.004717320973380587 56-57 0.0027446231117850685 58-59 7.719252501895505E-4 60-61 0.003945395723191036 62-63 0.008834255641058188 64-65 0.014752349225844744 66-67 0.0049746293901104365 68-69 0.00480309044562387 70-71 0.0 72-73 0.001543850500379101 74-75 0.01012079772470744 76-77 0.00506039886235372 78-79 0.0058323241125432706 80-81 0.008233869335355207 82-83 0.008491177752085056 84-85 0.009691950363491023 86-87 0.006518479890489538 88-89 0.01543850500379101 90-91 0.01921236178249548 92-93 0.0018869283893522347 94-95 0.003602317834217903 96-97 0.0018011589171089515 98-99 0.006518479890489538 100-101 0.007633483029652222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 582958.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.092246170704755 #Duplication Level Percentage of deduplicated Percentage of total 1 81.94278350215181 41.86640866604706 2 10.508556843942312 10.738115463390898 3 3.066136031921817 4.69967330807452 4 1.3265283696005679 2.711012560482234 5 0.7117810967694763 1.818324750790015 6 0.47111109525031736 1.4442074431367733 7 0.3241517648966954 1.1593149238139262 8 0.22765813881493063 0.9305252536877531 9 0.17702585560494458 0.8140183733832718 >10 1.0978541347379058 10.436031194582949 >50 0.08510055920809072 2.972374108705159 >100 0.04925111062222712 4.80126402042372 >500 0.0033504156885868774 1.2271913064181992 >1k 0.007705956083749819 9.393345759295894 >5k 6.700831377173755E-4 2.232361811549691 >10k+ 3.3504156885868775E-4 2.7558310562179646 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16099 2.7616054672892387 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7092 1.2165541942987317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5949 1.020485180750586 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4848 0.8316208028708758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4233 0.7261243520116373 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4223 0.7244089625667716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4033 0.6918165631143238 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3726 0.6391541071569479 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3265 0.5600746537486405 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3143 0.5391469025212794 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3100 0.531770727908357 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3072 0.5269676374627332 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2307 0.3957403449305096 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2260 0.3876780145396409 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1816 0.31151472318760526 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1786 0.3063685548530083 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1518 0.2603961177306084 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1511 0.2591953451192024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1431 0.2454722295602771 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1340 0.2298621856119995 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1288 0.22094216049869803 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1250 0.21442368060820846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.21099290171847715 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1217 0.20876289544015178 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1215 0.20841981755117864 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1062 0.18217435904473392 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 934 0.16021737415045337 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 868 0.14889580381433998 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.14495040809114895 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 836 0.1434065575907698 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 806 0.13826038925617284 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.12282188425238182 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.10086489935810126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.7153894448656678E-4 0.0 20-21 0.0 0.0 0.0 1.7153894448656678E-4 0.0 22-23 0.0 0.0 0.0 8.576947224328339E-4 0.0 24-25 0.0 0.0 0.0 0.0017153894448656678 0.0 26-27 0.0 0.0 0.0 0.002230006278325368 0.0 28-29 0.0 0.0 0.0 0.004460012556650736 8.576947224328339E-5 30-31 0.0 0.0 0.0 0.007890791446382071 1.7153894448656678E-4 32-33 0.0 0.0 0.0 0.01286542083649251 1.7153894448656678E-4 34-35 0.0 0.0 0.0 0.01844043653230593 1.7153894448656678E-4 36-37 0.0 0.0 0.0 0.02650276692317457 1.7153894448656678E-4 38-39 0.0 0.0 0.0 0.03859626250947752 1.7153894448656678E-4 40-41 0.0 0.0 0.0 0.06218286737638046 1.7153894448656678E-4 42-43 0.0 0.0 0.0 0.09691950363491023 1.7153894448656678E-4 44-45 0.0 0.0 0.0 0.1315703704211967 1.7153894448656678E-4 46-47 0.0 0.0 0.0 0.2070475059952861 1.7153894448656678E-4 48-49 0.0 0.0 0.0 0.2764350090401024 1.7153894448656678E-4 50-51 0.0 0.0 0.0 0.3337290164986157 1.7153894448656678E-4 52-53 0.0 0.0 0.0 0.3950541891525633 1.7153894448656678E-4 54-55 0.0 0.0 0.0 0.45243396608331987 3.4307788897313356E-4 56-57 0.0 0.0 0.0 0.57233968827943 3.4307788897313356E-4 58-59 0.0 0.0 0.0 0.7060542955067088 3.4307788897313356E-4 60-61 0.0 0.0 0.0 0.8419131395400699 3.4307788897313356E-4 62-63 0.0 0.0 0.0 0.9731404320722934 3.4307788897313356E-4 64-65 0.0 0.0 0.0 1.1266677873877706 3.4307788897313356E-4 66-67 0.0 0.0 0.0 1.3487249510256314 3.4307788897313356E-4 68-69 0.0 0.0 0.0 1.6044895172551024 3.4307788897313356E-4 70-71 0.0 0.0 0.0 1.8056189296656018 3.4307788897313356E-4 72-73 0.0 0.0 0.0 1.9901090644609045 3.4307788897313356E-4 74-75 0.0 0.0 0.0 2.190981168454674 3.4307788897313356E-4 76-77 0.0 0.0 0.0 2.41055101739748 3.4307788897313356E-4 78-79 0.0 0.0 0.0 2.660311720569921 3.4307788897313356E-4 80-81 0.0 0.0 0.0 3.013167329378789 3.4307788897313356E-4 82-83 0.0 0.0 0.0 3.36636601607663 3.4307788897313356E-4 84-85 0.0 0.0 0.0 3.7971860751546425 3.4307788897313356E-4 86-87 0.0 0.0 0.0 4.232294607844819 3.4307788897313356E-4 88-89 0.0 0.0 0.0 4.770583815643665 3.4307788897313356E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 95 7.2759576E-12 49.995617 2 TCACGCA 100 1.2732926E-11 47.495842 4 ATCACGC 100 1.2732926E-11 47.495842 3 TATTCCG 60 8.2899984E-5 39.57987 5 GTAAGGT 220 0.0 34.54243 4 GGTAAGG 205 0.0 32.436184 3 CACGCAG 150 9.531504E-10 31.663895 5 ACCGTGC 95 3.4920544E-5 29.994799 8 ATAGTCC 80 4.4955153E-4 29.682354 8 GTACACG 115 3.8551716E-6 28.917953 1 CATTCCG 100 4.9548296E-5 28.49506 9 GCTTATG 135 4.212834E-7 28.152927 1 GTATATA 340 0.0 27.94592 1 AAGGTAA 240 0.0 27.713037 1 AGGTAAG 225 3.6379788E-12 27.442041 2 TAAGGTG 295 0.0 27.370483 5 TACCTGG 500 0.0 25.647755 2 GTAAGCG 65 1.0014703E-5 25.579071 94-95 GTGTAGC 210 1.2587407E-9 24.885176 1 TATAGGT 175 1.4847865E-7 24.426432 3 >>END_MODULE