##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 614854 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82052975177847 31.0 31.0 34.0 30.0 34.0 2 31.919750054484478 31.0 31.0 34.0 30.0 34.0 3 31.975460841110248 31.0 31.0 34.0 30.0 34.0 4 35.70054354367053 37.0 35.0 37.0 33.0 37.0 5 35.56592784628546 37.0 35.0 37.0 33.0 37.0 6 35.707197155747544 37.0 35.0 37.0 33.0 37.0 7 35.65013970796319 37.0 35.0 37.0 33.0 37.0 8 35.639189140836685 37.0 35.0 37.0 33.0 37.0 9 37.19491456508374 39.0 37.0 39.0 33.0 39.0 10-11 37.08092571569836 39.0 37.0 39.0 33.0 39.0 12-13 36.97994971163887 39.0 37.0 39.0 33.0 39.0 14-15 37.8877863687965 39.5 37.0 41.0 33.0 41.0 16-17 37.897087763924446 39.5 37.0 41.0 33.0 41.0 18-19 38.157008818353624 40.0 37.0 41.0 33.0 41.0 20-21 38.198992769015085 40.0 37.5 41.0 33.0 41.0 22-23 38.09440533850312 40.0 37.0 41.0 33.0 41.0 24-25 37.88901755538713 40.0 37.0 41.0 33.0 41.0 26-27 37.59837457347598 39.5 36.5 41.0 32.5 41.0 28-29 37.515902962329264 39.0 36.0 41.0 32.0 41.0 30-31 37.39536784342299 39.0 36.0 41.0 32.0 41.0 32-33 37.07774203306801 39.0 35.0 41.0 31.5 41.0 34-35 36.75492068035663 39.0 35.0 41.0 31.0 41.0 36-37 36.44216431868379 38.5 35.0 40.5 30.5 41.0 38-39 36.21627573375143 38.0 35.0 40.0 30.0 41.0 40-41 36.0392711115159 38.0 35.0 40.0 30.0 41.0 42-43 35.61817195626929 38.0 35.0 40.0 29.0 41.0 44-45 35.12052536049208 38.0 35.0 40.0 26.5 41.0 46-47 34.450684227475136 38.0 34.0 40.0 23.5 41.0 48-49 33.791356159348396 38.0 33.0 40.0 19.5 41.0 50-51 33.40188158489657 38.0 33.0 40.0 16.5 41.0 52-53 33.098829152937114 38.0 33.0 40.0 13.0 41.0 54-55 32.91652164578908 37.0 33.0 40.0 10.5 41.0 56-57 32.519972221047595 37.0 32.0 40.0 8.0 41.0 58-59 32.145461361559 36.0 31.0 39.0 6.5 41.0 60-61 31.596970175033423 36.0 31.0 39.0 2.0 40.0 62-63 31.341136269748592 35.0 31.0 39.0 2.0 40.0 64-65 30.942971827458226 35.0 30.0 38.5 2.0 40.0 66-67 30.466214743662725 35.0 29.5 38.0 2.0 40.0 68-69 30.045218051765133 34.5 29.0 37.0 2.0 39.5 70-71 29.59748330497972 34.0 29.0 37.0 2.0 39.0 72-73 29.1124632839666 34.0 28.0 36.0 2.0 39.0 74-75 28.63664788714069 34.0 27.5 35.5 2.0 38.5 76-77 27.199501832955463 31.5 26.0 34.5 2.0 36.0 78-79 27.95656204562384 33.0 27.0 35.0 2.0 37.0 80-81 27.65934514535158 33.0 26.0 35.0 2.0 36.5 82-83 27.329243202451313 33.0 26.0 35.0 2.0 36.0 84-85 26.956222940730644 33.0 25.0 35.0 2.0 36.0 86-87 26.669930585147043 33.0 25.0 35.0 2.0 35.0 88-89 26.162510124354725 32.5 24.5 35.0 2.0 35.0 90-91 25.585152735446137 32.0 21.5 34.0 2.0 35.0 92-93 24.900798400921197 32.0 19.0 34.0 2.0 35.0 94-95 24.323760600077417 31.0 13.0 34.0 2.0 35.0 96-97 23.613382201303075 31.0 2.0 34.0 2.0 35.0 98-99 22.831390053573692 31.0 2.0 34.0 2.0 35.0 100-101 19.905571078662575 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19371646703675438 1101 2 0.21799573203458067 1101 3 0.2606046247786793 1101 4 0.12178114103322457 1101 5 0.1745035150626606 1101 6 0.1493035375509848 1101 7 -0.02079237635283704 1101 8 0.07703745704356635 1101 9 0.02131446375017987 1101 10-11 0.18703197505850966 1101 12-13 0.12839118388377813 1101 14-15 0.3245430205512605 1101 16-17 0.23403622082923903 1101 18-19 0.2574201834209404 1101 20-21 0.07799904436959793 1101 22-23 0.007156654416263564 1101 24-25 0.12998549036203855 1101 26-27 0.2805108394422362 1101 28-29 0.21981756479059555 1101 30-31 0.22552686472579353 1101 32-33 0.1996786671886639 1101 34-35 0.2815893127767808 1101 36-37 0.42570128743791 1101 38-39 0.3709142531366112 1101 40-41 0.3841584417396149 1101 42-43 0.3169465867536232 1101 44-45 0.5379588036757639 1101 46-47 0.6997762213560108 1101 48-49 0.8624169118272889 1101 50-51 0.7494559001865042 1101 52-53 0.7377805463541449 1101 54-55 0.7254730836202086 1101 56-57 0.5457888382985061 1101 58-59 0.5676936338061189 1101 60-61 0.7580365582739255 1101 62-63 0.7135707197977688 1101 64-65 0.824605962485041 1101 66-67 0.5511557069653179 1101 68-69 0.3854874962725887 1101 70-71 0.5089711385121731 1101 72-73 0.801328503606257 1101 74-75 0.7891804690735604 1101 76-77 0.8189168174008579 1101 78-79 0.7775024588686286 1101 80-81 0.7913044055814247 1101 82-83 1.0565783737699128 1101 84-85 0.943166010075938 1101 86-87 1.1339056432480206 1101 88-89 0.9718086515561559 1101 90-91 1.1509208920215954 1101 92-93 1.0311800016909274 1101 94-95 1.0187960254441357 1101 96-97 1.138539391827372 1101 98-99 1.0961739384182962 1101 100-101 1.5791952029798537 1102 1 0.2858708639383245 1102 2 0.24605288635432743 1102 3 0.17927081824543478 1102 4 0.21143812253213667 1102 5 0.2825520283405467 1102 6 0.3763878031395791 1102 7 0.24275399981023327 1102 8 0.3078875195368198 1102 9 0.2861701120597786 1102 10-11 0.27788905839826583 1102 12-13 0.36609390118283613 1102 14-15 0.48895350090060674 1102 16-17 0.48334339443702845 1102 18-19 0.36706806096503186 1102 20-21 0.43631296107612627 1102 22-23 0.3722433734435313 1102 24-25 0.2758212025536224 1102 26-27 0.39236218266133704 1102 28-29 0.3622878997852723 1102 30-31 0.4329681905085039 1102 32-33 0.3935817177850467 1102 34-35 0.37458775430315683 1102 36-37 0.2419182856016846 1102 38-39 0.3502806796028821 1102 40-41 0.5121623610693149 1102 42-43 0.5135538205400962 1102 44-45 0.2152448221614236 1102 46-47 0.15613895994906102 1102 48-49 0.320757831789642 1102 50-51 -0.01505410644858074 1102 52-53 0.17898905778037744 1102 54-55 0.09001858024866038 1102 56-57 -0.09679722493540055 1102 58-59 0.3206450896673694 1102 60-61 0.6225704820784479 1102 62-63 0.5759239378143413 1102 64-65 0.3665667074662906 1102 66-67 0.32690678323794486 1102 68-69 0.4541193570283717 1102 70-71 0.5570323047442152 1102 72-73 0.5731965116918012 1102 74-75 0.5506734405118294 1102 76-77 0.5685935498526007 1102 78-79 0.6869505123196404 1102 80-81 0.679549502561688 1102 82-83 0.7522246774099521 1102 84-85 0.8391510116961349 1102 86-87 0.7773820424024329 1102 88-89 0.688904412816516 1102 90-91 0.7741047301872293 1102 92-93 0.7763348460750237 1102 94-95 0.6019594954396617 1102 96-97 0.9874596473553261 1102 98-99 1.3227227680200357 1102 100-101 1.7793225040430123 1103 1 0.21080222863153253 1103 2 0.2765808199389994 1103 3 0.14466089009904692 1103 4 0.25224493220544986 1103 5 0.2721910526026363 1103 6 0.15524418786510097 1103 7 0.1027478259166088 1103 8 0.07069178083701644 1103 9 0.1631749854949618 1103 10-11 0.24091137636692395 1103 12-13 0.14132969137553886 1103 14-15 0.40345164216454066 1103 16-17 0.1811969812064973 1103 18-19 0.16064983878367656 1103 20-21 0.06532270794649264 1103 22-23 0.02897488788649838 1103 24-25 0.10177815606551377 1103 26-27 0.193470856325078 1103 28-29 0.259857093369682 1103 30-31 0.22665833986417994 1103 32-33 0.43258397716739694 1103 34-35 0.3334853858924802 1103 36-37 0.31947289828838166 1103 38-39 0.3443809988063222 1103 40-41 0.5463782108992064 1103 42-43 0.6114560103019429 1103 44-45 0.6019390962034805 1103 46-47 0.7763476244176459 1103 48-49 0.8334701414826498 1103 50-51 0.6426609617926502 1103 52-53 0.7257283881099568 1103 54-55 0.7896652233581989 1103 56-57 0.6716029484590536 1103 58-59 0.6719281429861645 1103 60-61 0.6642787459007486 1103 62-63 0.73188535754716 1103 64-65 0.3753973457418063 1103 66-67 0.6081949595748526 1103 68-69 0.697876499697859 1103 70-71 0.6789355059737403 1103 72-73 0.6825171206741629 1103 74-75 0.5652165723835694 1103 76-77 0.48850862597539546 1103 78-79 0.8013563735778 1103 80-81 0.8161863068492572 1103 82-83 0.8152673560916277 1103 84-85 1.0686375933294592 1103 86-87 1.1779544411571479 1103 88-89 1.0484039990342815 1103 90-91 0.8394856068801211 1103 92-93 0.8259722940238454 1103 94-95 0.9133651071148883 1103 96-97 1.074160160970731 1103 98-99 1.1279709059999874 1103 100-101 1.4817933750874488 1104 1 0.13114236835101423 1104 2 0.13049494094541103 1104 3 0.12662576268134274 1104 4 0.1781235666181047 1104 5 0.11684471709628497 1104 6 0.16004547371536404 1104 7 0.16380775444057605 1104 8 0.14507534494095609 1104 9 0.2158759893920177 1104 10-11 0.18982082808468448 1104 12-13 0.25850953082384365 1104 14-15 0.28871195500688884 1104 16-17 0.4213070686658682 1104 18-19 0.17227367370636415 1104 20-21 0.12978268675509952 1104 22-23 0.16112159334418408 1104 24-25 0.03169213340150634 1104 26-27 0.16681550370399378 1104 28-29 0.17997409496800998 1104 30-31 0.2180273393792973 1104 32-33 0.3614258139937192 1104 34-35 0.15395393104988386 1104 36-37 0.3551952013252091 1104 38-39 0.4706695428862062 1104 40-41 0.4679970173571917 1104 42-43 0.18082861953635643 1104 44-45 0.4948265124705671 1104 46-47 0.5781699939020513 1104 48-49 0.8210650986509052 1104 50-51 0.9143547462511066 1104 52-53 0.9894924633214188 1104 54-55 0.9510178803630254 1104 56-57 0.9712576904808614 1104 58-59 1.0267806114235718 1104 60-61 1.2036897869725287 1104 62-63 1.1946352093870338 1104 64-65 1.243182845804661 1104 66-67 1.0437996324552685 1104 68-69 0.9348029404427898 1104 70-71 0.9304929831211659 1104 72-73 1.1231794413106222 1104 74-75 0.9836512831570587 1104 76-77 0.9167798218520566 1104 78-79 0.8779834410849396 1104 80-81 1.0622281867767072 1104 82-83 1.0833994610428697 1104 84-85 1.1725270209824252 1104 86-87 1.2967582053221527 1104 88-89 1.1222927981291626 1104 90-91 1.317650845821987 1104 92-93 1.4138393270500806 1104 94-95 1.5373032929166577 1104 96-97 1.820743049823701 1104 98-99 1.7925961057916666 1104 100-101 2.264765736490393 1105 1 -0.010432004578966314 1105 2 0.1059869984101276 1105 3 -0.02214646692437583 1105 4 0.19623528950484115 1105 5 0.20550788187488678 1105 6 0.21939087379552547 1105 7 0.07331242714061403 1105 8 0.2621902954715125 1105 9 0.18288651615193174 1105 10-11 0.12142062134671505 1105 12-13 0.05786716641652845 1105 14-15 0.27334214718881356 1105 16-17 0.15357770554539485 1105 18-19 0.2253241135519417 1105 20-21 0.16806454655300485 1105 22-23 0.1549732483027313 1105 24-25 0.17518199691225078 1105 26-27 0.05507415821952577 1105 28-29 -0.06227850507839605 1105 30-31 0.25576703939828604 1105 32-33 0.19935115627163214 1105 34-35 0.35211333024403046 1105 36-37 0.10539561058201485 1105 38-39 0.28510639287460293 1105 40-41 0.4986780923946412 1105 42-43 0.5764444033475087 1105 44-45 0.7945090220163777 1105 46-47 0.47324783707653495 1105 48-49 0.5704955144473729 1105 50-51 0.7010934547716587 1105 52-53 0.8782827297602509 1105 54-55 0.6619359657162818 1105 56-57 0.5162036854600771 1105 58-59 0.4794840268192253 1105 60-61 0.8462461652608262 1105 62-63 0.7132432088807406 1105 64-65 0.8904356568081866 1105 66-67 1.0078150956116083 1105 68-69 1.0438936098097074 1105 70-71 1.1842986494292056 1105 72-73 1.4171581979293997 1105 74-75 1.3362328708202824 1105 76-77 1.0737203108506428 1105 78-79 1.2882011488249567 1105 80-81 1.0866100824373213 1105 82-83 1.3054866707131438 1105 84-85 1.2508079314733216 1105 86-87 1.3027921061301164 1105 88-89 0.9522671668399987 1105 90-91 1.1617287522836044 1105 92-93 1.1774682112979171 1105 94-95 1.333861527627544 1105 96-97 1.1929062040544487 1105 98-99 0.6847110563222252 1105 100-101 1.2797410545082393 1106 1 -0.05786248587647336 1106 2 0.030409278107345727 1106 3 -0.06277652049552529 1106 4 0.14798306790887494 1106 5 0.184176873294021 1106 6 -0.050309823103461326 1106 7 -0.18691455227900633 1106 8 -0.03840862678448076 1106 9 -0.10274198348724184 1106 10-11 -0.054067682022953534 1106 12-13 -0.05685383694944335 1106 14-15 -0.06140838088467149 1106 16-17 0.0751021654881896 1106 18-19 -0.08373583661688144 1106 20-21 -0.05592618779837011 1106 22-23 -0.060682221274447556 1106 24-25 -0.050010400936464805 1106 26-27 -0.23029293845610965 1106 28-29 -0.23635312541857445 1106 30-31 -0.2782541941603256 1106 32-33 -0.4534299868955429 1106 34-35 -0.24669698997130496 1106 36-37 -0.2873343581813188 1106 38-39 -0.06133747330814998 1106 40-41 -0.01613496168746309 1106 42-43 0.16739353973006388 1106 44-45 -0.06649176285095848 1106 46-47 -0.42224957918234196 1106 48-49 -0.3826376338188098 1106 50-51 -0.3313554103312413 1106 52-53 -0.4883418402746642 1106 54-55 -0.682590506436739 1106 56-57 -1.0113463432481566 1106 58-59 -0.7560262159628977 1106 60-61 -0.8686073173904489 1106 62-63 -0.8615805910360343 1106 64-65 -0.797054795728414 1106 66-67 -0.8472418994526834 1106 68-69 -0.7233609611207008 1106 70-71 -0.6218114637707899 1106 72-73 -0.5786521117508023 1106 74-75 -0.7141902464199319 1106 76-77 -0.9241134026253697 1106 78-79 -0.9250602119980478 1106 80-81 -0.7694332643634247 1106 82-83 -0.8350862297667305 1106 84-85 -0.5316797819289469 1106 86-87 -0.5207543090278293 1106 88-89 -0.6461500612614124 1106 90-91 -0.6405946393967987 1106 92-93 -0.5327682497405313 1106 94-95 -0.6099901693188521 1106 96-97 -0.4093438367691924 1106 98-99 -0.3129626548360598 1106 100-101 0.11964589592816566 1107 1 -0.052615963453547465 1107 2 -0.021532210036060206 1107 3 0.06150009766574982 1107 4 0.07899190881830975 1107 5 -0.03315222536075879 1107 6 0.01727656281216383 1107 7 -0.15333894647057633 1107 8 0.05964759468106706 1107 9 -0.04373998321239725 1107 10-11 0.027132024806995503 1107 12-13 0.05708777334815096 1107 14-15 0.09908008317908923 1107 16-17 0.0845309419612903 1107 18-19 0.10874404611509902 1107 20-21 -0.010849001620115928 1107 22-23 0.06740484412384973 1107 24-25 0.25345572417995754 1107 26-27 0.09176505424517956 1107 28-29 -0.018582744755583747 1107 30-31 -0.1803766231963735 1107 32-33 -0.33061540168536396 1107 34-35 -0.3400899752669986 1107 36-37 -0.3018096295926611 1107 38-39 -0.14226080628520066 1107 40-41 0.052333224780909404 1107 42-43 -0.0505234259598808 1107 44-45 -0.035492083505701544 1107 46-47 -0.1816814095101691 1107 48-49 -0.3143229035505257 1107 50-51 -0.2445549339299049 1107 52-53 -0.16868952163546425 1107 54-55 -0.2717271817050886 1107 56-57 -0.4783723468211676 1107 58-59 -0.5840033425909823 1107 60-61 -0.052761628669863114 1107 62-63 -0.25573169576798094 1107 64-65 -0.3055941370119477 1107 66-67 -0.19292145847605724 1107 68-69 -0.10142588607222436 1107 70-71 -0.34065298397967325 1107 72-73 0.030771320476524977 1107 74-75 -0.09279952097674027 1107 76-77 -0.23370326993538626 1107 78-79 -0.21862268623114645 1107 80-81 0.25222696375952225 1107 82-83 0.31002760266826 1107 84-85 0.5724291796077488 1107 86-87 0.5098964674147339 1107 88-89 0.2730357810680708 1107 90-91 0.4504980298249244 1107 92-93 0.5571708258576429 1107 94-95 0.619630694616653 1107 96-97 0.8078279883670909 1107 98-99 0.8426113935064592 1107 100-101 1.2926963914160936 1108 1 -0.012908473836471757 1108 2 0.030752033749084973 1108 3 0.03677979084779537 1108 4 0.040001785018063174 1108 5 -0.07704269294019639 1108 6 -0.03736843053084016 1108 7 -0.15641308751939675 1108 8 -0.12357677588507698 1108 9 -0.09801536425088386 1108 10-11 -0.10050444360704347 1108 12-13 0.006108102024903417 1108 14-15 0.06077453700735447 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-0.1833773228346054 2316 86-87 -0.30348133604132954 2316 88-89 -0.8458832798069764 2316 90-91 -1.1205534959905101 2316 92-93 -0.7586966041544407 2316 94-95 -0.6851594729341635 2316 96-97 -1.213402118256159 2316 98-99 -1.4533757906943912 2316 100-101 -2.150577940218973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 8.0 5 33.0 6 97.0 7 192.0 8 413.0 9 735.0 10 1368.0 11 2188.0 12 3193.0 13 4198.0 14 5158.0 15 7103.0 16 9726.0 17 12619.0 18 10507.0 19 6141.0 20 5050.0 21 5125.0 22 5488.0 23 5862.0 24 6680.0 25 8003.0 26 9409.0 27 11231.0 28 13615.0 29 16636.0 30 19803.0 31 23944.0 32 29184.0 33 37294.0 34 48615.0 35 64765.0 36 85357.0 37 97908.0 38 54195.0 39 3006.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.51885230167587 18.860760729170394 14.711107062333461 17.909279906820274 2 15.92055095773475 24.378318238600873 37.352793826774935 22.348336976889442 3 23.15705063037888 27.79392604617194 29.898550559622894 19.150472763826286 4 12.090512544441445 17.580921649692446 38.62982106321176 31.698744742654355 5 11.858714232506395 41.17891025130156 31.95291775570515 15.00945776048689 6 27.84612281940103 38.32080461377823 16.617115607932938 17.2159569588878 7 26.86052298594463 36.552905242545386 19.61359932601886 16.972972445491123 8 27.330390629320135 29.94483243176429 19.125678616386978 23.5990983225286 9 26.833199426205244 19.016221737192897 21.421996116151153 32.72858272045071 10-11 25.914932650678047 26.866296714341942 25.153044462587864 22.065726172392143 12-13 30.093096331282414 25.547493929384057 25.575468368553476 18.783941370780045 14-15 23.92443716175831 26.202463192391672 29.640116938615357 20.23298270723466 16-17 19.648273652401468 29.565603485711218 29.12932034161011 21.656802520277203 18-19 19.54435670778814 28.47091543397116 32.69214192194781 19.292585936292888 20-21 22.552020479658587 24.20037927703162 32.38402937933233 20.863570863977465 22-23 20.93264568929484 25.512683519475388 32.87704540760149 20.677625383628282 24-25 22.727427974771246 25.334876247044015 33.002956799500375 18.934738978684372 26-27 21.56143291282119 24.24812412223884 35.451019809041455 18.739423155898514 28-29 19.410187536747394 28.29242428308526 33.2198890930072 19.077499087160145 30-31 20.449982231337415 25.550198301527296 32.9053674372384 21.094452029896892 32-33 20.54984360589662 26.167994755958507 33.18507204864076 20.09708958950411 34-35 22.224679938878754 24.99912579379956 33.39394496323048 19.382249304091204 36-37 21.81579837618403 24.36870576142396 34.63067294680962 19.184822915582387 38-39 20.409497585593908 26.24652151496867 34.209914271913036 19.134066627524394 40-41 20.241147659231537 26.96569822144046 32.52761032542004 20.26554379390796 42-43 19.906102446411058 27.478087046756848 30.823380539638496 21.7924299671936 44-45 21.543850004716628 25.278037101973506 31.306619869040375 21.87149302426949 46-47 23.15734261469214 27.29264335755324 30.483815223976485 19.066198803778143 48-49 22.594572000605027 27.33280854323582 29.925152354480534 20.14746710167862 50-51 19.96605695503075 28.057659263548146 31.86019108616931 20.116092695251798 52-53 21.97469140872456 29.547751851872935 28.002660809416273 20.474895929986232 54-55 20.205762688983782 27.679481226248694 29.32073590535339 22.794020179414133 56-57 22.572306991573672 26.94911669974298 28.825438396720564 21.65313791196278 58-59 24.57242795843247 26.634128485144288 28.08866654464849 20.704777011774755 60-61 21.700932481324344 27.44019885993909 31.309052812410528 19.549815846326037 62-63 20.86604374546555 27.752961424216494 30.56131229889807 20.81968253141989 64-65 20.563986771062268 30.009695581515277 29.004917742100783 20.42139990532167 66-67 21.89381112471923 27.22082074853333 28.145468089501307 22.739900037246127 68-69 21.297585395922855 27.901314484046306 28.738196154459196 22.062903965571643 70-71 23.268301370330885 27.749464708144977 28.282845532620478 20.69938838890366 72-73 22.32728798590883 26.612702548414013 30.51396024740873 20.54604921826843 74-75 20.611022244052705 30.592830474994653 28.272546367867335 20.523600913085303 76-77 21.664784052345624 27.851469455700823 28.039812210238008 22.443934281715546 78-79 21.81379446080975 28.313160903928704 28.39587301535664 21.477171619904908 80-81 22.714188691829374 27.878874144444943 28.364288532003755 21.042648631721928 82-83 21.980457658123704 27.30624605000842 29.510514196381582 21.202782095486295 84-85 20.61444147341653 28.583421664243836 29.282937396636566 21.519199465703068 86-87 20.811587837975217 28.85280566704485 27.8459455173171 22.489660977662833 88-89 20.48687779565842 28.753158717735516 26.73361331274438 24.026350173861683 90-91 21.66366027717365 26.865568419159853 27.767892085669487 23.702879217997005 92-93 22.704913165252435 28.411057123263934 27.76548069656525 21.11854901491838 94-95 21.497951599834696 28.139388831582135 28.056736942465825 22.305922626117347 96-97 19.195570928850234 28.40612186943386 30.06620986174613 22.33209733996978 98-99 20.893025405510087 29.44205847937989 26.59653068564445 23.068385429465575 100-101 19.83886022210631 27.964692795256784 27.655465870705715 24.540981111931195 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1210.0 1 881.0 2 1086.0 3 1378.0 4 897.5 5 703.5 6 1024.5 7 1979.5 8 2874.5 9 3147.0 10 2702.0 11 2030.0 12 1880.5 13 1879.5 14 1935.0 15 1991.5 16 2005.0 17 2080.0 18 2166.0 19 2267.5 20 2400.5 21 2570.0 22 2853.5 23 3194.5 24 3520.5 25 4019.5 26 4608.0 27 5183.5 28 5880.5 29 6662.0 30 7504.5 31 8538.0 32 9860.5 33 10763.0 34 11527.0 35 12138.0 36 12905.0 37 14194.5 38 14461.0 39 13939.0 40 13961.0 41 14538.0 42 15075.5 43 15840.5 44 16690.0 45 17405.0 46 18183.5 47 27874.5 48 48647.0 49 46289.5 50 26357.0 51 17767.5 52 14645.0 53 14009.0 54 13800.0 55 13092.5 56 12108.5 57 11309.5 58 10961.0 59 10352.5 60 9112.0 61 7887.5 62 6901.5 63 5313.5 64 3559.0 65 2597.0 66 1920.0 67 1484.5 68 1150.0 69 771.5 70 579.5 71 472.0 72 413.5 73 395.0 74 303.0 75 240.0 76 188.5 77 125.5 78 85.0 79 54.5 80 44.0 81 39.5 82 31.5 83 20.5 84 11.0 85 6.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020817950277626885 2 0.011384816558077201 3 0.010571615375357401 4 0.0 5 0.0024396035481594005 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011384816558077202 14-15 2.4396035481594002E-4 16-17 1.6264023654396004E-4 18-19 0.0014637621288956402 20-21 0.0 22-23 1.6264023654396004E-4 24-25 0.0 26-27 0.0013824420106236602 28-29 0.0023582834298874204 30-31 0.0012198017740797003 32-33 0.009921054429181562 34-35 0.0017077224837115804 36-37 9.758414192637601E-4 38-39 4.8792070963188004E-4 40-41 4.066005913599001E-4 42-43 0.005692408279038601 44-45 0.0013011218923516803 46-47 2.4396035481594002E-4 48-49 8.132011827198002E-4 50-51 8.132011827198002E-5 52-53 5.692408279038601E-4 54-55 0.00317148461260722 56-57 0.018540986966011443 58-59 0.031145605298168344 60-61 0.024396035481594004 62-63 0.018866267439099364 64-65 0.022932273352698363 66-67 0.004066005913599001 68-69 0.0019516828385275201 70-71 4.066005913599001E-4 72-73 1.6264023654396004E-4 74-75 0.0025209236664313806 76-77 0.0028462041395193004 78-79 0.011547456794621163 80-81 0.019760788740091143 82-83 0.022363032524794504 84-85 0.03488633073867943 86-87 0.011628776912893142 88-89 0.04635246741502861 90-91 0.053589957941234834 92-93 0.034073129555959626 94-95 0.03708197393202289 96-97 0.05993292716644927 98-99 0.06196593012324877 100-101 0.06806493899364727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 614854.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.570478413757755 #Duplication Level Percentage of deduplicated Percentage of total 1 81.61259791739268 41.27188121272198 2 10.614561740673544 10.735669307564608 3 3.204493366287389 4.86158287820599 4 1.356130709567818 2.743207150977333 5 0.7643903085157908 1.9327791798241716 6 0.45658794651362744 1.3853922535889625 7 0.3260960062118316 1.154358173006363 8 0.237084786339675 0.9591592855856723 9 0.1782268939549112 0.8111717364148137 >10 1.1023399511628305 10.462182369534101 >50 0.08510290619095086 2.9637247628363546 >100 0.05086978093868267 5.035808023264044 >500 0.003199357291741041 1.2406289452976271 >1k 0.00703858604183029 8.648160978618776 >5k 9.598071875223123E-4 3.0624184502599747 >10k+ 3.199357291741041E-4 2.7318752922992218 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 16885 2.746180394044765 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7394 1.2025619090060404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6355 1.033578703236866 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5179 0.8423137850611689 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4617 0.7509099721234634 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4438 0.7217973697820946 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4375 0.7115510348798251 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3937 0.6403146112735706 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3464 0.5633857793882775 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3300 0.5367127805950681 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3236 0.5263038054562547 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3220 0.5237015616715514 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2634 0.42839438305679073 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2475 0.40253458544630105 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1966 0.3197507050454254 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1955 0.3179616624434418 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1619 0.26331454296467127 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1605 0.26103757965305585 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1580 0.25697157373945684 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1495 0.24314715363322023 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1415 0.23013593470970345 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1325 0.21549831342074702 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1282 0.20850478324935676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1237 0.20118597260487858 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1196 0.1945177229065762 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1081 0.1758140957040208 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 995 0.16182703536124024 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 960 0.15613462708220163 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 959 0.15597198684565766 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 830 0.13499139633148682 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 815 0.13255179278332743 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.11742625078473914 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 717 0.11661304960201935 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 617 0.10034902594762334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.6264023654396004E-4 0.0 14-15 0.0 0.0 0.0 1.6264023654396004E-4 0.0 16-17 0.0 0.0 0.0 1.6264023654396004E-4 0.0 18-19 0.0 0.0 0.0 1.6264023654396004E-4 0.0 20-21 0.0 0.0 0.0 1.6264023654396004E-4 0.0 22-23 0.0 0.0 0.0 2.4396035481594007E-4 0.0 24-25 0.0 0.0 0.0 4.879207096318801E-4 0.0 26-27 0.0 0.0 0.0 0.0013824420106236602 0.0 28-29 0.0 0.0 0.0 0.0026835639029753403 8.132011827198002E-5 30-31 0.0 0.0 0.0 0.007644091117566121 1.6264023654396004E-4 32-33 0.0 0.0 0.0 0.011466136676349183 1.6264023654396004E-4 34-35 0.0 0.0 0.0 0.016182703536124023 1.6264023654396004E-4 36-37 0.0 0.0 0.0 0.021875111815162624 1.6264023654396004E-4 38-39 0.0 0.0 0.0 0.03488633073867943 1.6264023654396004E-4 40-41 0.0 0.0 0.0 0.05480975971531453 1.6264023654396004E-4 42-43 0.0 0.0 0.0 0.08579272477693892 1.6264023654396004E-4 44-45 0.0 0.0 0.0 0.11783285137609904 1.6264023654396004E-4 46-47 0.0 0.0 0.0 0.18784947320827383 1.6264023654396004E-4 48-49 0.0 0.0 0.0 0.24932748262189072 1.6264023654396004E-4 50-51 0.0 0.0 0.0 0.30039651689669417 1.6264023654396004E-4 52-53 0.0 0.0 0.0 0.3569953192139923 1.6264023654396004E-4 54-55 0.0 0.0 0.0 0.41286224046684256 1.6264023654396004E-4 56-57 0.0 0.0 0.0 0.5345171374017246 1.6264023654396004E-4 58-59 0.0 0.0 0.0 0.6591808787126701 1.6264023654396004E-4 60-61 0.0 0.0 0.0 0.7827874584860797 1.6264023654396004E-4 62-63 0.0 0.0 0.0 0.9102160838182722 1.6264023654396004E-4 64-65 0.0 0.0 0.0 1.05057460795571 1.6264023654396004E-4 66-67 0.0 0.0 0.0 1.2528177420981241 1.6264023654396004E-4 68-69 0.0 0.0 0.0 1.4884021247320502 1.6264023654396004E-4 70-71 0.0 0.0 0.0 1.6764142381768679 1.6264023654396004E-4 72-73 0.0 0.0 0.0 1.8404369167314516 1.6264023654396004E-4 74-75 0.0 0.0 0.0 2.030888633724429 1.6264023654396004E-4 76-77 0.0 0.0 0.0 2.2332130879851153 1.6264023654396004E-4 78-79 0.0 0.0 0.0 2.4796130463492148 1.6264023654396004E-4 80-81 0.0 0.0 0.0 2.810667247834445 1.6264023654396004E-4 82-83 0.0 0.0 0.0 3.1470072570073544 1.6264023654396004E-4 84-85 0.0 0.0 0.0 3.5668630276455873 1.6264023654396004E-4 86-87 0.0 0.0 0.0 3.9843605148539325 1.6264023654396004E-4 88-89 0.0 0.0 0.0 4.490496930978736 1.6264023654396004E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGTG 260 0.0 34.707024 5 AGGTAAG 220 0.0 34.54658 2 GTAAGGT 235 0.0 32.336216 4 ACGACGG 60 0.003952052 31.662548 6 TACCTGG 500 0.0 30.40099 2 GGTAAGG 250 0.0 30.396044 3 TAAGGGT 125 2.1536107E-7 30.396044 3 GTCCTAT 100 4.937348E-5 28.510202 1 CGACGGT 70 0.008393914 27.139326 7 GTAAGCG 90 6.673872E-9 26.398336 94-95 ATGGGAG 1475 0.0 26.081352 5 GCTTATG 130 9.983418E-6 25.586079 1 AAGGTGT 335 0.0 25.519068 6 AAGGTAA 280 0.0 25.455538 1 GGGCGTA 95 0.0012268359 24.996748 9 GTACCTG 610 0.0 24.926952 1 GGGTACC 115 1.2819379E-4 24.779385 7 GTACATG 5780 0.0 24.333967 1 GAGTACT 5930 0.0 24.307545 12-13 TACATGG 5815 0.0 23.93457 2 >>END_MODULE