##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 324509 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780492990949405 33.0 31.0 34.0 30.0 34.0 2 31.87235484994253 33.0 31.0 34.0 30.0 34.0 3 31.864838879661274 33.0 31.0 34.0 30.0 34.0 4 35.51247577108801 37.0 35.0 37.0 33.0 37.0 5 35.53226566905695 37.0 35.0 37.0 33.0 37.0 6 35.548786012098276 37.0 35.0 37.0 33.0 37.0 7 35.495601046504106 37.0 35.0 37.0 33.0 37.0 8 35.47387899873347 37.0 35.0 37.0 33.0 37.0 9 37.031274941527045 39.0 37.0 39.0 33.0 39.0 10-11 36.937707428761605 39.0 37.0 39.0 33.0 39.0 12-13 36.912931536567555 39.0 37.0 39.0 33.0 39.0 14-15 38.041535057579296 40.0 38.0 41.0 33.0 41.0 16-17 37.973430629042646 40.0 38.0 41.0 33.0 41.0 18-19 38.054277385218896 40.0 38.0 41.0 33.0 41.0 20-21 37.973151746176526 40.0 38.0 41.0 33.0 41.0 22-23 38.03977855775957 40.0 38.0 41.0 33.0 41.0 24-25 38.07662961581959 40.0 38.0 41.0 33.0 41.0 26-27 37.98885393009131 40.0 37.5 41.0 33.0 41.0 28-29 37.88240541864786 40.0 37.0 41.0 33.0 41.0 30-31 37.69313331833632 40.0 37.0 41.0 33.0 41.0 32-33 37.461962534167 39.5 36.0 41.0 33.0 41.0 34-35 37.27396928898736 39.0 36.0 41.0 32.0 41.0 36-37 37.09508673103058 39.0 35.5 41.0 32.0 41.0 38-39 36.89172411242832 39.0 35.0 41.0 31.0 41.0 40-41 36.776155669026124 39.0 35.0 41.0 31.0 41.0 42-43 36.556214157388546 39.0 35.0 41.0 31.0 41.0 44-45 36.2681173711669 39.0 35.0 40.5 30.0 41.0 46-47 35.97050158855379 39.0 35.0 41.0 29.5 41.0 48-49 35.78392586954445 39.0 35.0 41.0 28.5 41.0 50-51 34.81907897777874 38.0 34.0 40.0 26.5 40.5 52-53 34.97256008307936 38.5 35.0 39.5 27.0 40.5 54-55 35.46142325790656 39.0 35.0 40.5 27.5 41.0 56-57 35.37645643110052 39.0 35.0 41.0 27.0 41.0 58-59 35.207367130033376 39.0 35.0 41.0 26.0 41.0 60-61 34.94261638352095 39.0 35.0 40.5 26.0 41.0 62-63 34.613519193612504 38.0 34.5 40.0 25.0 41.0 64-65 34.27436989420941 38.0 34.0 40.0 23.0 41.0 66-67 33.77938362264221 37.5 34.0 40.0 22.0 41.0 68-69 33.39249296629677 37.0 33.5 39.0 21.0 41.0 70-71 32.935786064485114 36.5 33.0 39.0 19.0 41.0 72-73 32.49732056738026 36.0 32.5 39.0 16.0 40.0 74-75 32.02247549374594 35.5 32.0 38.0 10.5 40.0 76-77 31.516118197029975 35.0 31.5 37.0 7.0 39.0 78-79 30.993662733545143 35.0 31.0 37.0 2.0 39.0 80-81 30.474888215735156 35.0 31.0 36.0 2.0 38.5 82-83 30.021125762305513 34.5 30.5 36.0 2.0 37.5 84-85 29.593333312789476 34.0 30.0 35.5 2.0 37.0 86-87 29.28188586449066 34.0 30.0 35.0 2.0 36.5 88-89 28.8683487977221 34.0 29.0 35.0 2.0 36.0 90-91 28.446043098958732 34.0 29.0 35.0 2.0 36.0 92-93 28.03859369077591 34.0 29.0 35.0 2.0 35.5 94-95 27.588065354119607 34.0 27.5 35.0 2.0 35.0 96-97 27.094820174478983 34.0 26.5 35.0 2.0 35.0 98-99 26.524937675072188 34.0 25.5 35.0 2.0 35.0 100-101 23.77491841520574 30.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20383156819566395 1101 2 0.2632026241776053 1101 3 0.2880298002360142 1101 4 0.32111637498596934 1101 5 0.12576070131651562 1101 6 0.2828410565691968 1101 7 0.3533595575875097 1101 8 0.4488579556399941 1101 9 0.5708559149483037 1101 10-11 0.6007216358805536 1101 12-13 0.6762047390294512 1101 14-15 0.5205085416627853 1101 16-17 0.41057855528033116 1101 18-19 0.5552972438550228 1101 20-21 0.4756891283981517 1101 22-23 0.3366748937493469 1101 24-25 0.43695063175696447 1101 26-27 0.4596667617064085 1101 28-29 0.48286618497294853 1101 30-31 0.5939839052506883 1101 32-33 0.39799184672769883 1101 34-35 0.36149227591862143 1101 36-37 0.4446357642736416 1101 38-39 0.4940940534506453 1101 40-41 0.27172548515432027 1101 42-43 0.6104836109610687 1101 44-45 0.7613131296658509 1101 46-47 0.9398364912902082 1101 48-49 0.948877098445287 1101 50-51 0.9150486989398132 1101 52-53 1.0973732505409046 1101 54-55 0.8850530139298343 1101 56-57 0.9991749616566281 1101 58-59 1.0674378981598736 1101 60-61 0.9887564608807864 1101 62-63 1.0427898574302148 1101 64-65 0.9296237285246178 1101 66-67 1.1320837018181962 1101 68-69 1.2471727622952145 1101 70-71 0.9433837255106994 1101 72-73 0.8043403251887611 1101 74-75 0.8397249881106461 1101 76-77 0.8933178431342839 1101 78-79 0.7427537892951861 1101 80-81 0.5798894491517288 1101 82-83 0.4929760248548689 1101 84-85 0.3124217899203394 1101 86-87 0.5407804211649214 1101 88-89 0.47784642836129976 1101 90-91 0.5702192005557976 1101 92-93 0.626989496141622 1101 94-95 0.6004681561362162 1101 96-97 0.3409550489631492 1101 98-99 0.21104715899428683 1101 100-101 0.28902215941824494 1102 1 0.07372781823631769 1102 2 0.1694731546358632 1102 3 -0.09627110716958498 1102 4 0.06982356593142924 1102 5 0.15571484318401474 1102 6 0.19219481465942323 1102 7 0.015407887505872964 1102 8 0.1904132293252303 1102 9 0.15659761332538125 1102 10-11 0.09873770248867686 1102 12-13 0.25199099459910457 1102 14-15 -0.11494044523023916 1102 16-17 -0.3560685972873969 1102 18-19 0.12748965287053693 1102 20-21 -0.13472489573427993 1102 22-23 -0.21155420177217366 1102 24-25 0.02884902834849612 1102 26-27 -0.22925410862240625 1102 28-29 -0.3334736205365374 1102 30-31 0.08340401127729535 1102 32-33 -0.1341023413231639 1102 34-35 -0.1818355284200095 1102 36-37 -0.043811889155961126 1102 38-39 -0.08757732912325622 1102 40-41 -0.05784487943409289 1102 42-43 0.06726150799167385 1102 44-45 -0.0205208703991957 1102 46-47 0.1856344553192173 1102 48-49 0.08151768126637648 1102 50-51 0.3528442562299574 1102 52-53 0.44818959034640926 1102 54-55 0.4484510690246353 1102 56-57 0.37670122438144915 1102 58-59 0.17036338682543573 1102 60-61 0.6262723160861725 1102 62-63 0.6076091893975573 1102 64-65 0.6812938101585999 1102 66-67 0.7541915497383229 1102 68-69 0.7992884158549103 1102 70-71 0.9011291966135744 1102 72-73 0.8341042045916254 1102 74-75 0.7287987188811229 1102 76-77 0.8185609562835268 1102 78-79 0.7769180980082311 1102 80-81 1.00543015230976 1102 82-83 0.7938948136797954 1102 84-85 0.7231708060845179 1102 86-87 0.8359197843548252 1102 88-89 0.9066362289922552 1102 90-91 1.097688547158718 1102 92-93 0.9534311002005573 1102 94-95 0.9846568574729311 1102 96-97 1.0116708911591346 1102 98-99 1.2135091878101036 1102 100-101 1.2830492189687703 1103 1 0.2305277956731402 1103 2 0.16393812530373353 1103 3 0.1868262529387188 1103 4 0.3888950912021869 1103 5 0.4842911067219191 1103 6 0.27820282458721834 1103 7 0.33444415781273307 1103 8 0.2424707090183702 1103 9 0.058236264047401676 1103 10-11 0.452750422479653 1103 12-13 0.03534571594386904 1103 14-15 0.3629582179015287 1103 16-17 0.4573198278028556 1103 18-19 0.5001557742604277 1103 20-21 0.561074122767522 1103 22-23 0.17766412516826335 1103 24-25 0.25961392004524697 1103 26-27 0.12475861136856992 1103 28-29 0.34742312472519643 1103 30-31 0.28727641651195057 1103 32-33 0.16742983940788037 1103 34-35 0.17271306702222944 1103 36-37 0.301471701773643 1103 38-39 0.4802743743965934 1103 40-41 0.31464496713630297 1103 42-43 -0.13783071899388233 1103 44-45 0.12098197820188261 1103 46-47 0.287295669218139 1103 48-49 0.5822051530624037 1103 50-51 0.4349037102010769 1103 52-53 0.6835975607886482 1103 54-55 0.6990539289073112 1103 56-57 0.41896134960708054 1103 58-59 0.48714686493915593 1103 60-61 0.5735273645970054 1103 62-63 0.8037783850203013 1103 64-65 0.8510781907993916 1103 66-67 0.9130468915704526 1103 68-69 0.8331085027231424 1103 70-71 0.5350099220197109 1103 72-73 0.5905417609995709 1103 74-75 0.5282297037637989 1103 76-77 0.8213284709495952 1103 78-79 0.5676798872681594 1103 80-81 0.5061211496021798 1103 82-83 0.39627170334586026 1103 84-85 0.7213340224653848 1103 86-87 0.4017372764577125 1103 88-89 0.26032812881175005 1103 90-91 0.23696680753777954 1103 92-93 0.2929892849929736 1103 94-95 0.6681307405956751 1103 96-97 0.8991440973865714 1103 98-99 0.7211066325303221 1103 100-101 0.642953043427255 1104 1 0.2497964406311226 1104 2 0.334286733497013 1104 3 0.29577451974543933 1104 4 0.31674435590807093 1104 5 0.3273845941168787 1104 6 0.4105229401334398 1104 7 0.11219822819088421 1104 8 0.5684128046066022 1104 9 0.5069298039633665 1104 10-11 0.7040224156519201 1104 12-13 0.45532503579681105 1104 14-15 0.5445413980485867 1104 16-17 0.3788993971305885 1104 18-19 0.4829715568234505 1104 20-21 0.27531059860785945 1104 22-23 0.3287030563657396 1104 24-25 0.4947767351712997 1104 26-27 0.32666085664168065 1104 28-29 0.7088579330235163 1104 30-31 0.5886485278564848 1104 32-33 0.45653337144569406 1104 34-35 0.6382051232197057 1104 36-37 0.8061903861223811 1104 38-39 0.47218285653944747 1104 40-41 0.32849738310268606 1104 42-43 0.8464950425607398 1104 44-45 0.7177273169623817 1104 46-47 0.8906635788819983 1104 48-49 1.1172547197640839 1104 50-51 1.1126544980162052 1104 52-53 1.0392386453474742 1104 54-55 1.085009483003951 1104 56-57 1.2802976810146447 1104 58-59 1.1832717992879012 1104 60-61 0.8448886434835643 1104 62-63 1.1885522164278584 1104 64-65 1.6472556461565304 1104 66-67 1.3183695675158305 1104 68-69 1.4370754801391072 1104 70-71 1.5435692051079428 1104 72-73 1.297293993142432 1104 74-75 1.3643067126923683 1104 76-77 1.3474987046680837 1104 78-79 1.5510208799152174 1104 80-81 1.7757180508626824 1104 82-83 1.7640090326525808 1104 84-85 1.5809962467300913 1104 86-87 1.7415564072350236 1104 88-89 1.8757758704083898 1104 90-91 1.7082936195125704 1104 92-93 1.8697274021148225 1104 94-95 1.9038351786208842 1104 96-97 2.082655783310944 1104 98-99 2.435318489147967 1104 100-101 2.2104261264104466 1105 1 0.08836786920255335 1105 2 -0.16831830851979745 1105 3 0.1107325029387205 1105 4 -0.09796152644486966 1105 5 0.10528375378073918 1105 6 0.09850613341074421 1105 7 0.29858478281273193 1105 8 0.19900103990072182 1105 9 0.5287785434591683 1105 10-11 0.4304089702737741 1105 12-13 0.3212073887379816 1105 14-15 0.32512963334270495 1105 16-17 0.1272187248616774 1105 18-19 0.13011441396630374 1105 20-21 -0.058470913997183516 1105 22-23 -0.21381795203761556 1105 24-25 -0.3935846093665205 1105 26-27 -0.45111225260202303 1105 28-29 -0.19429332748068617 1105 30-31 -0.31534306878216256 1105 32-33 -0.17476914956270662 1105 34-35 0.010978232463401127 1105 36-37 0.009341646626587874 1105 38-39 -0.2446658829563475 1105 40-41 -0.5651160622754645 1105 42-43 -0.40329487340564185 1105 44-45 -0.31323906959223535 1105 46-47 -0.20400028666421122 1105 48-49 -0.1810646079670093 1105 50-51 -0.250748863328333 1105 52-53 -0.16899664877017528 1105 54-55 -0.030410685063280596 1105 56-57 -0.10449223495174209 1105 58-59 0.09965835390973865 1105 60-61 0.14732561827347723 1105 62-63 0.3117034769320597 1105 64-65 0.13733967976997974 1105 66-67 -0.05213463416483677 1105 68-69 0.27863010198785076 1105 70-71 0.21003979334324185 1105 72-73 0.2680923124701593 1105 74-75 0.6846428471461508 1105 76-77 0.37804492315547833 1105 78-79 0.31572676226815943 1105 80-81 0.2962222525433589 1105 82-83 0.24631995702232956 1105 84-85 0.380996246730092 1105 86-87 0.048867331604771636 1105 88-89 0.030649819988219917 1105 90-91 0.0074953001848392375 1105 92-93 0.055987906316502745 1105 94-95 0.3411040861839112 1105 96-97 0.6408490606218642 1105 98-99 0.39884790091267774 1105 100-101 -0.41491000804333567 1106 1 0.11340781110524034 1106 2 -0.07239964475799354 1106 3 0.015423860963409197 1106 4 0.060789381325641045 1106 5 -0.08611784389536581 1106 6 -0.005313764918952302 1106 7 0.21068354824483038 1106 8 0.39760670438874257 1106 9 0.3711895819486344 1106 10-11 0.33438863621421433 1106 12-13 0.30543396825868285 1106 14-15 0.258012712500296 1106 16-17 0.11705169508680768 1106 18-19 0.06564092086536988 1106 20-21 0.031478250853943734 1106 22-23 0.12102591143369779 1106 24-25 0.1005475620205587 1106 26-27 0.315656084362395 1106 28-29 0.11042707920050532 1106 30-31 0.11820812947491532 1106 32-33 0.22508560715479575 1106 34-35 0.2601576079172929 1106 36-37 0.018709838347710672 1106 38-39 0.2684350611249897 1106 40-41 0.5203741338029673 1106 42-43 0.4147879152653786 1106 44-45 0.17142323977595453 1106 46-47 -0.15569237090531374 1106 48-49 0.03142631432783105 1106 50-51 -0.498998681774232 1106 52-53 -0.1129036930693772 1106 54-55 -0.10290160735812037 1106 56-57 0.10147726395893386 1106 58-59 0.3936307576860685 1106 60-61 0.3407751462328079 1106 62-63 0.09090463887832811 1106 64-65 0.2525466514475383 1106 66-67 0.4522371886383567 1106 68-69 0.09485377664289274 1106 70-71 -0.005631956838314522 1106 72-73 0.06576841995018867 1106 74-75 0.2731250548440478 1106 76-77 0.17406525721502675 1106 78-79 0.29597367584840484 1106 80-81 0.4019448378737849 1106 82-83 -0.13578607057389291 1106 84-85 -0.13389118972597203 1106 86-87 -0.18988357616574447 1106 88-89 -0.3062710224228198 1106 90-91 -0.4576753606720949 1106 92-93 -0.3216591525070278 1106 94-95 -0.016615594280867896 1106 96-97 -0.28747337946527907 1106 98-99 -0.08625013830301143 1106 100-101 -0.15999352293076896 1107 1 0.0380658024298981 1107 2 0.0785179394929223 1107 3 0.2610027732089897 1107 4 0.14814340201299814 1107 5 0.4527877283435373 1107 6 0.2954536691818106 1107 7 0.1920982963262432 1107 8 0.3731822799643183 1107 9 0.14563068972307747 1107 10-11 0.11333424849316032 1107 12-13 0.29602455884927537 1107 14-15 0.24032836148261083 1107 16-17 0.08715513185691037 1107 18-19 0.3147567033144796 1107 20-21 0.26532876803779004 1107 22-23 0.22181002888447665 1107 24-25 0.25767135247767925 1107 26-27 0.2799370319766794 1107 28-29 0.08511843722519785 1107 30-31 -0.07853861727183897 1107 32-33 0.23357743231328953 1107 34-35 0.5218526362789859 1107 36-37 0.5162573858952655 1107 38-39 0.4522021615587519 1107 40-41 0.5712750347038735 1107 42-43 0.6663394668169289 1107 44-45 0.5270788954316146 1107 46-47 0.6245211759748912 1107 48-49 0.7268050263732135 1107 50-51 0.5168505007416186 1107 52-53 0.6870128901805401 1107 54-55 1.1395575184343372 1107 56-57 1.0036794661611381 1107 58-59 0.9277982585202338 1107 60-61 0.8860537581780861 1107 62-63 0.8139610286013763 1107 64-65 0.8935876924885804 1107 66-67 0.8600116297461255 1107 68-69 0.9075331226555718 1107 70-71 0.7969873291143017 1107 72-73 0.5894754603238965 1107 74-75 0.6915267899124444 1107 76-77 0.87620072601716 1107 78-79 0.6427537892951882 1107 80-81 0.7019615212238008 1107 82-83 0.7042372861161326 1107 84-85 0.44935872685727674 1107 86-87 1.0295191599036606 1107 88-89 0.621540122054995 1107 90-91 0.4035525338891297 1107 92-93 0.4026651718172971 1107 94-95 0.3842519399199986 1107 96-97 0.4873514453595469 1107 98-99 0.9551913031384274 1107 100-101 1.104787925184013 1108 1 0.2990081607173849 1108 2 0.26307506298809713 1108 3 0.3854227684254461 1108 4 0.5550317062464316 1108 5 0.36892421881631776 1108 6 0.40056408933648413 1108 7 0.4841895025767471 1108 8 0.541690611304503 1108 9 0.23809660696775836 1108 10-11 0.7558679326197719 1108 12-13 0.43840676406333756 1108 14-15 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-1.1604774285985826 2316 84-85 -0.96660723910869 2316 86-87 -1.329389749005248 2316 88-89 -1.2328142322993614 2316 90-91 -1.6440951137585138 2316 92-93 -2.5123998932477676 2316 94-95 -2.4400723844043277 2316 96-97 -2.4837696757615753 2316 98-99 -3.345509397562271 2316 100-101 -3.5890058686097817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 476.0 3 135.0 4 107.0 5 118.0 6 143.0 7 181.0 8 217.0 9 298.0 10 378.0 11 638.0 12 988.0 13 1183.0 14 1535.0 15 2188.0 16 2886.0 17 4230.0 18 3042.0 19 2102.0 20 2024.0 21 2298.0 22 2319.0 23 2577.0 24 2957.0 25 3312.0 26 3593.0 27 4256.0 28 4837.0 29 5822.0 30 7119.0 31 8709.0 32 10948.0 33 14682.0 34 19410.0 35 28322.0 36 40365.0 37 62869.0 38 66070.0 39 11174.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.97087603051082 24.17566838739502 16.475229216426534 21.378226365667615 2 13.120326937182941 24.714453902720162 39.82451165915689 22.340707500940006 3 16.179339635144256 30.822882539134618 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32.132583269004684 18.925102964356537 48-49 17.222533805021754 31.467945320300274 31.91153993121895 19.39798094345902 50-51 16.452078139397283 31.58596227391933 32.33435131911852 19.627608267564877 52-53 16.93697996918336 32.4613251155624 31.24375963020031 19.35793528505393 54-55 17.038835849044975 31.101037307315387 31.93633780593786 19.923789037701773 56-57 17.603626424083302 31.557562702807644 30.722449746846753 20.1163611262623 58-59 17.64269909459035 30.677300658871964 31.604898703828116 20.07510154270957 60-61 17.785180608350373 31.803032660966622 31.410275176771684 19.001511553911318 62-63 17.29341033194867 31.879491414695476 32.068392766896345 18.7587054864595 64-65 17.081987066594557 31.280844723635898 31.9621828087708 19.67498540099874 66-67 17.103847872651905 31.100735056169388 30.929376049805064 20.866041021373647 68-69 16.55106507598878 31.132124911372117 32.07265945312741 20.2441505595117 70-71 18.055831749393786 30.529259332910954 31.663529618513365 19.751379299181902 72-73 17.352082289941055 29.851369572754944 32.208806872905186 20.58774126439881 74-75 16.598594371320242 31.67072531672883 31.393606855522332 20.33707345642859 76-77 16.960410810277654 30.234995314888792 32.25390836908813 20.550685505745424 78-79 16.718928735504598 29.873245212393662 32.334958236594545 21.072867815507188 80-81 17.831882627768742 30.853076549453768 30.695182218384513 20.61985860439298 82-83 17.575914527463958 30.040248303204915 31.91115468389436 20.472682485436774 84-85 16.753888781017015 29.7828076891071 31.309811100940333 22.15349242893555 86-87 16.887013581424593 30.593264612013233 31.709921852485973 20.8097999540762 88-89 17.55109463178886 29.19798919392073 31.077494937539875 22.17342123675053 90-91 16.905785083203344 27.963290647561855 32.92034798049237 22.210576288742423 92-93 17.80446984197435 29.746702790337842 31.83830033812003 20.61052702956778 94-95 16.974805454965715 28.2035595962709 32.312504815471144 22.509130133292242 96-97 16.03018614377715 28.60425299773311 32.582172792095015 22.78338806639472 98-99 16.538903233265252 29.463177771783112 31.41269413079502 22.585224864156615 100-101 16.15898736519145 28.905882625018307 31.965526013521327 22.969603996268916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 593.0 1 403.5 2 268.0 3 313.5 4 278.5 5 304.0 6 491.0 7 904.0 8 1356.0 9 1531.5 10 1320.0 11 1035.0 12 982.5 13 989.0 14 927.0 15 843.5 16 792.0 17 758.5 18 732.5 19 743.5 20 800.5 21 870.5 22 952.5 23 1034.0 24 1122.0 25 1377.5 26 1972.0 27 2685.0 28 2995.5 29 3704.0 30 5629.0 31 7279.5 32 9774.0 33 13533.0 34 16231.5 35 18026.5 36 20897.0 37 23008.0 38 21616.5 39 19261.5 40 16654.0 41 14500.5 42 12011.0 43 10974.0 44 10898.5 45 9760.5 46 8456.0 47 8860.0 48 14211.0 49 13170.0 50 6034.0 51 2626.5 52 1349.0 53 962.0 54 788.5 55 709.0 56 623.5 57 564.5 58 566.5 59 532.5 60 452.0 61 404.5 62 362.5 63 273.5 64 185.0 65 131.5 66 79.0 67 57.5 68 54.0 69 45.0 70 32.0 71 27.0 72 22.5 73 16.0 74 14.5 75 13.0 76 10.0 77 8.5 78 6.0 79 7.5 80 7.5 81 3.0 82 1.0 83 1.0 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010477367345743878 2 0.013250788113734903 3 0.01602420888172593 4 0.018181313923496728 5 0.028350523406130493 6 0.047456310918957564 7 0.006779472988422509 8 0.024652629048809122 9 0.03204841776345186 10-11 0.03774933823098897 12-13 0.029274996995460836 14-15 0.02064657682837764 16-17 0.027117891953690035 18-19 0.02234144507548327 20-21 0.04884302130295307 22-23 0.011864077729739391 24-25 0.0035438154257663116 26-27 9.244735893303422E-4 28-29 3.08157863110114E-4 30-31 0.002465262904880912 32-33 0.0029274996995460835 34-35 0.0 36-37 3.08157863110114E-4 38-39 1.54078931555057E-4 40-41 0.0010785525208853992 42-43 0.00416013115198654 44-45 0.0015407893155505701 46-47 0.0010785525208853992 48-49 3.08157863110114E-4 50-51 0.003697894357321369 52-53 0.0027734207679910266 54-55 0.004622367946651711 56-57 6.16315726220228E-4 58-59 0.004622367946651711 60-61 0.0026193418364359694 62-63 3.08157863110114E-4 64-65 1.54078931555057E-4 66-67 0.013558945976845017 68-69 0.03667078571010357 70-71 0.049151179166063194 72-73 0.014329340634620304 74-75 0.030507628447901294 76-77 0.023728155459478782 78-79 0.05608473108604076 80-81 0.07442012394109254 82-83 0.04545328480874182 84-85 0.015407893155505704 86-87 0.01802723499194167 88-89 0.01910578751282707 90-91 0.03790341716254403 92-93 0.020954734691487755 94-95 0.010477367345743878 96-97 0.01710276140261133 98-99 0.045145126945631706 100-101 0.06363459873223855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 324509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.091248624845534 #Duplication Level Percentage of deduplicated Percentage of total 1 72.9877416244174 15.394026051665746 2 9.20620078021127 3.883405390913657 3 3.7052730008912524 2.3444650225417476 4 2.220826088862265 1.8735998077094935 5 1.4596087255088175 1.5392485262350197 6 1.0417427640518389 1.3182993383850679 7 0.8444983416857824 1.2468067141435213 8 0.6823195944070248 1.151277776579386 9 0.588811127507561 1.1176885695003838 >10 5.655801177622255 25.566317112930616 >50 0.9979106701927152 14.535498245040971 >100 0.5654340107826952 20.710057348178324 >500 0.03214353549669068 4.32283850370868 >1k 0.011688558362432974 4.9964715924673895 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3002 0.9250899050565624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2416 0.7445093972740355 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2272 0.7001346649861792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2223 0.6850349296937835 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1844 0.5682430995750504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1639 0.505070737637477 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1612 0.49675047533350386 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1206 0.37163838291079754 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 983 0.3029191794372421 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 893 0.2751849717573318 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 800 0.24652629048809127 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 715 0.22033287212373157 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 714 0.22002471426062145 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 698 0.21509418845085962 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 653 0.2012270846109045 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 623 0.19198234871760103 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 607 0.18705182290783923 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 603 0.18581919145539877 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 602 0.18551103359228865 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 600 0.18489471786606843 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 585 0.1802723499194167 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 575 0.1771907712883156 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 573 0.17657445556209533 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 568 0.1750336662465448 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.17472550838343467 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 563 0.17349287693099422 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 537 0.16548077249013124 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 534 0.16455629890080092 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 525 0.16178287813280987 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 510 0.15716051018615818 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 458 0.14113630130443222 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 452 0.13928735412577156 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 447 0.13774656481022096 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 435 0.1340486704528996 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 408 0.12572840814892655 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 403 0.12418761883337597 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 400 0.12326314524404564 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 398 0.12264682951782539 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 384 0.1183326194342838 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 377 0.116175514392513 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 375 0.11555919866629277 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 366 0.11278577789830173 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 359 0.11062867285653094 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 358 0.11032051499342084 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 357 0.11001235713031073 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 357 0.11001235713031073 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 356 0.1097041992672006 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 351 0.10816340995165002 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 350 0.10785525208853992 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 341 0.1050818313205489 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 330 0.10169209482633763 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 327 0.1007676212370073 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.5407893155505704E-4 0.0 18-19 0.0 0.0 0.0 3.0815786311011407E-4 0.0 20-21 0.0 0.0 0.0 4.622367946651711E-4 0.0 22-23 0.0 0.0 0.0 0.0016948682471056274 0.0 24-25 0.0 0.0 0.0 0.0036978943573213684 0.0 26-27 0.0 0.0 0.0 0.006471315125312395 0.0 28-29 0.0 0.0 0.0 0.011247762003519162 0.0 30-31 0.0 0.0 0.0 0.0197221032390473 0.0 32-33 0.0 0.0 0.0 0.028658681269240607 0.0 34-35 0.0 0.0 0.0 0.03682486464165863 0.0 36-37 0.0 0.0 0.0 0.045761442671851935 0.0 38-39 0.0 0.0 0.0 0.060861177964247525 0.0 40-41 0.0 0.0 0.0 0.07318749248865208 0.0 42-43 0.0 0.0 0.0 0.10847156781476014 0.0 44-45 0.0 0.0 0.0 0.14822393215596485 0.0 46-47 0.0 0.0 0.0 0.18227537602963245 0.0 48-49 0.0 0.0 0.0 0.2240307664810529 0.0 50-51 0.0 0.0 0.0 0.2767257610728824 0.0 52-53 0.0 0.0 0.0 0.394442064780946 0.0 54-55 0.0 0.0 0.0 0.48673534478242514 0.0 56-57 0.0 0.0 0.0 0.6497508543676754 0.0 58-59 0.0 0.0 0.0 0.8474341235528136 0.0 60-61 0.0 0.0 0.0 1.0303258153086663 0.0 62-63 0.0 0.0 0.0 1.2833234209220699 0.0 64-65 0.0 0.0 0.0 1.4296984058993742 0.0 66-67 0.0 0.0 0.0 1.7546508725489893 0.0 68-69 0.0 0.0 0.0 2.1416971486152927 0.0 70-71 0.0 0.0 0.0 2.3731237038109882 0.0 72-73 0.0 0.0 0.0 2.8162547109633325 0.0 74-75 0.0 0.0 0.0 3.285270978616926 0.0 76-77 0.0 0.0 0.0 3.5416583207245407 0.0 78-79 0.0 0.0 0.0 3.800973162531702 0.0 80-81 0.0 0.0 0.0 4.328077187381552 0.0 82-83 0.0 0.0 0.0 4.75518398565217 0.0 84-85 0.0 0.0 0.0 5.397230893442092 0.0 86-87 0.0 0.0 0.0 6.289347907145872 0.0 88-89 0.0 0.0 0.0 7.484384100286896 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACA 60 8.2722814E-5 39.587635 4 TCTTCGA 25 0.001613823 38.00706 20-21 AGGGTCT 50 0.0016132436 38.00413 5 GTATGGT 50 0.0016132436 38.00413 1 GGCATTC 90 5.67632E-7 36.948463 7 CATGGGT 120 3.772584E-9 35.628872 4 TAGGCAT 80 1.07442465E-5 35.628872 5 ACTATAC 150 2.5465852E-11 34.837124 3 ACAATCA 55 0.0025755332 34.54921 8 CCCTATA 125 5.6188583E-9 34.20372 2 CTTTAGG 85 1.6273945E-5 33.53306 2 GGATATG 90 2.405874E-5 31.67011 1 CCTTTAG 90 2.405874E-5 31.67011 1 GATTGGC 105 1.8945011E-6 31.67011 9 GCCTATC 60 0.0039447066 31.670109 7 CTACAGC 60 0.0039447066 31.670109 3 TACAATC 60 0.0039447066 31.670109 7 GTGGTAC 95 3.4806155E-5 30.003263 1 TGGTACT 95 3.4806155E-5 30.003263 2 ATGGGTA 80 4.4837504E-4 29.690727 5 >>END_MODULE