##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936591_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 324509 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.943681068937995 31.0 31.0 34.0 30.0 34.0 2 32.060611570095126 33.0 31.0 34.0 30.0 34.0 3 32.153478023721995 33.0 31.0 34.0 30.0 34.0 4 35.891818100576565 37.0 35.0 37.0 35.0 37.0 5 35.7663238924036 37.0 35.0 37.0 35.0 37.0 6 35.84577931582791 37.0 35.0 37.0 35.0 37.0 7 35.78196290395644 37.0 35.0 37.0 33.0 37.0 8 35.769377736827025 37.0 35.0 37.0 33.0 37.0 9 37.369194691056336 39.0 37.0 39.0 34.0 39.0 10-11 37.275271255959 39.0 37.0 39.0 34.0 39.0 12-13 37.20325630413949 39.0 37.0 39.0 33.0 39.0 14-15 38.213782976743325 40.0 37.5 41.0 33.0 41.0 16-17 38.186353845347895 40.0 37.5 41.0 33.0 41.0 18-19 38.42648740096577 40.0 38.0 41.0 34.0 41.0 20-21 38.48330554776601 40.0 38.0 41.0 34.0 41.0 22-23 38.36741507939688 40.0 38.0 41.0 34.0 41.0 24-25 38.23630623495805 40.0 37.5 41.0 33.5 41.0 26-27 38.00812304127159 40.0 37.0 41.0 33.0 41.0 28-29 38.03257074534142 40.0 37.0 41.0 33.0 41.0 30-31 37.8798723610131 40.0 37.0 41.0 33.0 41.0 32-33 37.615439017099675 40.0 36.0 41.0 33.0 41.0 34-35 37.41238763793916 39.0 36.0 41.0 33.0 41.0 36-37 37.20578936177425 39.0 35.5 41.0 32.5 41.0 38-39 37.00142368932757 39.0 35.0 41.0 32.0 41.0 40-41 36.839793349337 39.0 35.0 41.0 31.0 41.0 42-43 36.557301954645325 39.0 35.0 40.5 31.0 41.0 44-45 36.20936707456496 39.0 35.0 40.0 30.0 41.0 46-47 35.75933641285758 39.0 35.0 40.0 29.5 41.0 48-49 35.301413828275955 38.5 35.0 40.0 27.0 41.0 50-51 35.01711971008508 38.0 35.0 40.0 26.0 41.0 52-53 34.84687327624195 38.0 34.5 40.0 25.5 41.0 54-55 34.563542151373305 38.0 34.0 40.0 24.0 41.0 56-57 34.25114711764543 38.0 34.0 40.0 23.0 41.0 58-59 33.94716325279114 38.0 33.0 40.0 22.5 41.0 60-61 33.58452153869384 37.5 33.0 40.0 22.0 41.0 62-63 33.309988320816984 37.0 33.0 39.5 21.0 40.5 64-65 33.02143546095794 37.0 32.5 39.0 19.5 40.0 66-67 32.574890064682336 36.0 32.0 39.0 17.5 40.0 68-69 32.08515634389185 36.0 31.0 39.0 13.5 40.0 70-71 31.543028082426066 35.0 31.0 38.0 8.0 40.0 72-73 30.93425606069477 35.0 30.5 37.5 7.0 39.5 74-75 30.36938266735283 34.5 29.5 37.0 2.0 39.0 76-77 28.88316965014838 32.5 28.0 35.0 2.0 37.5 78-79 29.629450955135297 34.0 29.0 36.0 2.0 38.0 80-81 29.406164389893654 34.0 29.0 36.0 2.0 37.0 82-83 28.957079772826024 34.0 29.0 35.0 2.0 37.0 84-85 28.47245685019522 34.0 28.5 35.0 2.0 36.5 86-87 28.10214508688511 33.5 28.0 35.0 2.0 36.0 88-89 27.60223137108678 33.0 26.5 35.0 2.0 35.5 90-91 27.03524555559322 33.0 26.0 35.0 2.0 35.0 92-93 26.507286392673237 32.0 25.0 34.0 2.0 35.0 94-95 25.81092666151016 32.0 24.5 34.0 2.0 35.0 96-97 25.056993796782216 32.0 19.5 34.0 2.0 35.0 98-99 24.06815065221612 31.0 6.0 34.0 2.0 35.0 100-101 20.4562554505422 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2053965492430727 1101 2 0.27824002673635917 1101 3 0.27417577225908474 1101 4 0.1691780408805883 1101 5 0.25107297654987804 1101 6 0.18491668651362403 1101 7 0.08847019721726923 1101 8 0.18516094172566255 1101 9 0.3146032305237014 1101 10-11 0.2064919369865592 1101 12-13 0.21776892542077064 1101 14-15 0.3950103657046853 1101 16-17 0.1972579211186556 1101 18-19 0.33609181931725374 1101 20-21 0.08505340654141236 1101 22-23 0.13155488887660738 1101 24-25 0.1144595285370329 1101 26-27 -0.21469799838754966 1101 28-29 0.13622361351196588 1101 30-31 0.2689409708314159 1101 32-33 0.3167822351583496 1101 34-35 0.5757591602479195 1101 36-37 0.5184213061365526 1101 38-39 0.7185192535604301 1101 40-41 0.8389685486796381 1101 42-43 1.1118163922002537 1101 44-45 1.4080298147505488 1101 46-47 1.444716656015828 1101 48-49 1.202033008719873 1101 50-51 1.3428847724482438 1101 52-53 1.439135019156474 1101 54-55 1.2785865641551268 1101 56-57 1.2079405484680095 1101 58-59 1.6855987169480144 1101 60-61 1.3776414055167336 1101 62-63 1.4583152370528012 1101 64-65 1.819500362230066 1101 66-67 1.924458899748565 1101 68-69 1.782371207315471 1101 70-71 1.7293179721857932 1101 72-73 1.8408043479182332 1101 74-75 1.5756223102402451 1101 76-77 1.7805806572179073 1101 78-79 1.516587029742194 1101 80-81 1.5359209466013901 1101 82-83 1.0374494339215836 1101 84-85 1.2380027385949823 1101 86-87 1.2389588492754662 1101 88-89 1.5454683032764933 1101 90-91 1.645654143982643 1101 92-93 1.624522724473561 1101 94-95 1.6849142975455464 1101 96-97 1.6408830250757092 1101 98-99 0.9349715312977658 1101 100-101 1.0622195273910684 1102 1 0.030468914026624105 1102 2 0.20291560484153948 1102 3 0.18820966174423148 1102 4 0.17281416798241622 1102 5 0.15714511366894612 1102 6 0.06579612190569151 1102 7 0.03219804058409892 1102 8 0.04598964046049758 1102 9 -0.09767113770734426 1102 10-11 0.09353132294290845 1102 12-13 0.15612226070551571 1102 14-15 0.10169394459042991 1102 16-17 0.21492631153072494 1102 18-19 0.14683595695669993 1102 20-21 0.0028551432962231615 1102 22-23 -0.02199830330474839 1102 24-25 0.043670508470036395 1102 26-27 0.0915806629452689 1102 28-29 0.20403348114133024 1102 30-31 0.18589059193266166 1102 32-33 0.09610054263550438 1102 34-35 0.09890776011132374 1102 36-37 0.4805948515231009 1102 38-39 -0.029584626492905386 1102 40-41 -0.1095662677252136 1102 42-43 -0.15715456431583164 1102 44-45 0.1416526072181128 1102 46-47 0.025929457264730615 1102 48-49 0.20971831135101837 1102 50-51 0.27275110087052923 1102 52-53 0.4780152351125011 1102 54-55 0.5035093208468311 1102 56-57 0.3789360927487522 1102 58-59 0.2684295622337274 1102 60-61 0.546706680177671 1102 62-63 0.33465276591740434 1102 64-65 0.2988625764298263 1102 66-67 0.7831231596763573 1102 68-69 0.36915040528600684 1102 70-71 0.10111034217311143 1102 72-73 0.1467301292952783 1102 74-75 0.6932012610321934 1102 76-77 0.32366453531983197 1102 78-79 0.4900706216373365 1102 80-81 0.7229619587326575 1102 82-83 0.6767638385789461 1102 84-85 0.26117410490714477 1102 86-87 0.5557279649615445 1102 88-89 0.4837565915647808 1102 90-91 0.3384989743726017 1102 92-93 0.6809737603469763 1102 94-95 0.6157124241920471 1102 96-97 0.6613500625102517 1102 98-99 0.6085624507370575 1102 100-101 0.5931217292318998 1103 1 0.2383533341529791 1103 2 0.11148538146608544 1103 3 0.3154166224843067 1103 4 0.16452925146617048 1103 5 0.15039026258591548 1103 6 0.07810010430641512 1103 7 0.23394809699204444 1103 8 0.055593092626565976 1103 9 0.1208532305237 1103 10-11 0.1069846171667379 1103 12-13 0.3075211776730242 1103 14-15 0.2653545380019793 1103 16-17 0.40408682033037024 1103 18-19 0.34204269206500015 1103 20-21 0.4363678068792538 1103 22-23 0.06721085734507426 1103 24-25 0.06106883315414535 1103 26-27 0.18895487323407423 1103 28-29 0.203346009345303 1103 30-31 0.4497493182413237 1103 32-33 0.4513930178160095 1103 34-35 0.5170598359235967 1103 36-37 0.3941990369924113 1103 38-39 0.14560786560998196 1103 40-41 0.3917099619679263 1103 42-43 0.6676247396101616 1103 44-45 0.5278777877235257 1103 46-47 0.2838794516239389 1103 48-49 0.622109726062213 1103 50-51 0.4762357015022971 1103 52-53 0.5303547545168286 1103 54-55 0.5297777944479165 1103 56-57 0.5535574544364792 1103 58-59 0.5922323036597206 1103 60-61 0.6832526808545722 1103 62-63 0.756386746061807 1103 64-65 0.6024430705634032 1103 66-67 0.38949796224856215 1103 68-69 0.49673459413979515 1103 70-71 0.6225559367128248 1103 72-73 0.9565542775353499 1103 74-75 0.6829773215015074 1103 76-77 0.6748075012494361 1103 78-79 0.6207396198322819 1103 80-81 0.7052392883806711 1103 82-83 0.9119567537414035 1103 84-85 1.0504798641580457 1103 86-87 1.079923446685374 1103 88-89 1.0501628415647808 1103 90-91 0.7852040454466085 1103 92-93 1.0166644755996863 1103 94-95 1.260056822883385 1103 96-97 1.5365192863369685 1103 98-99 1.6611099744283955 1103 100-101 1.6816575200712514 1104 1 0.12370982049752044 1104 2 0.1529277396593649 1104 3 0.2397371319380852 1104 4 0.10854513907121088 1104 5 0.10013422266995065 1104 6 0.1125834288862464 1104 7 0.025850014008852895 1104 8 0.029759321618165302 1104 9 0.22720827254050846 1104 10-11 0.09234438607429496 1104 12-13 -0.0018275618227789892 1104 14-15 -0.016305782376171862 1104 16-17 0.12745702778835266 1104 18-19 0.09770511328348874 1104 20-21 -0.016814637973688207 1104 22-23 0.04140991196692312 1104 24-25 0.14460185573818052 1104 26-27 0.16889841315003906 1104 28-29 0.18510316533269844 1104 30-31 0.2326323933463641 1104 32-33 0.3822130020597072 1104 34-35 0.33021634852864423 1104 36-37 0.4522183384630054 1104 38-39 0.6008419123536797 1104 40-41 0.6938534756233921 1104 42-43 0.47886915925302276 1104 44-45 0.6396162331016768 1104 46-47 0.332026444796206 1104 48-49 0.4580308132470847 1104 50-51 0.43853546515776287 1104 52-53 0.6631508417017002 1104 54-55 0.566337340141196 1104 56-57 0.7358627984490838 1104 58-59 1.162856647147116 1104 60-61 0.9801851257075143 1104 62-63 0.9253074393391216 1104 64-65 1.1225815947230657 1104 66-67 1.499668983782179 1104 68-69 1.1751829265977776 1104 70-71 1.2122273521539988 1104 72-73 1.2780895322622392 1104 74-75 1.5671356723418448 1104 76-77 1.246145802194814 1104 78-79 1.2150804811768197 1104 80-81 1.2187716544521017 1104 82-83 1.4171432033212348 1104 84-85 1.4294271461958594 1104 86-87 1.5198771625467167 1104 88-89 1.5604372638336876 1104 90-91 1.7318870155096313 1104 92-93 1.4493883463980062 1104 94-95 1.474432807652292 1104 96-97 1.9586568913789861 1104 98-99 1.839131573693102 1104 100-101 1.9255929848821758 1105 1 -0.014105305552902792 1105 2 0.15149916823078868 1105 3 0.17940099748430782 1105 4 0.16182244999558293 1105 5 0.04005018905650104 1105 6 -0.06699640304652377 1105 7 0.0020685014038122063 1105 8 0.06253243086185734 1105 9 0.10989734817076169 1105 10-11 0.31726035246085615 1105 12-13 -0.1735082340916847 1105 14-15 0.13230766300197416 1105 16-17 -0.04161860246374971 1105 18-19 0.06972192000618094 1105 20-21 0.1464206561439596 1105 22-23 0.02590571028625277 1105 24-25 0.22687076330119993 1105 26-27 0.15986479970466405 1105 28-29 -0.13632540609587096 1105 30-31 0.12708617485896667 1105 32-33 0.26620459869835855 1105 34-35 -0.08621222289993113 1105 36-37 0.6578065737571208 1105 38-39 0.29983350899232875 1105 40-41 0.29666860167380804 1105 42-43 0.5793733609336869 1105 44-45 0.6939439641941192 1105 46-47 0.8304718229474659 1105 48-49 0.9631988804739748 1105 50-51 0.6710564735611158 1105 52-53 0.7359659677521222 1105 54-55 0.9419675922420367 1105 56-57 0.8752325463482507 1105 58-59 1.064369252189131 1105 60-61 0.7319498315898656 1105 62-63 0.6673242460618098 1105 64-65 0.6093042837986999 1105 66-67 0.8056353703367947 1105 68-69 0.529048472816271 1105 70-71 0.19046264627164788 1105 72-73 0.3285097003294659 1105 74-75 0.16276592444268445 1105 76-77 0.13211218874943498 1105 78-79 0.39491241394992826 1105 80-81 0.41520022588067107 1105 82-83 0.3184457243296386 1105 84-85 0.8540069781286341 1105 86-87 0.4053393474206679 1105 88-89 0.5584624739177251 1105 90-91 0.657853402064255 1105 92-93 0.35770767412909876 1105 94-95 0.7215756647951501 1105 96-97 0.8644972275134393 1105 98-99 0.665602161928394 1105 100-101 -0.19995323360521766 1106 1 -0.032841688995169704 1106 2 0.08698210214509672 1106 3 -0.061092829676184124 1106 4 -0.04029810192889016 1106 5 0.08217074098097754 1106 6 0.03943787436814006 1106 7 0.11262768804142809 1106 8 -0.02504926848454403 1106 9 0.23559088484469015 1106 10-11 0.054573351734639175 1106 12-13 0.09471253569771676 1106 14-15 0.1787274160883996 1106 16-17 0.10156222542295978 1106 18-19 0.13322228311438522 1106 20-21 -0.11414579265778713 1106 22-23 -0.1073840500623362 1106 24-25 -0.015336934592767193 1106 26-27 0.03778782076494025 1106 28-29 0.021408798697152065 1106 30-31 -0.04090220567842806 1106 32-33 0.09789668293947074 1106 34-35 0.07736076184952623 1106 36-37 0.06440061878253545 1106 38-39 -0.20106699935914918 1106 40-41 -0.2739777309333107 1106 42-43 -0.23382925344539274 1106 44-45 -0.0831947431406661 1106 46-47 -0.2965579519254433 1106 48-49 -0.18895798227111982 1106 50-51 -0.3672587043619018 1106 52-53 -0.26964042295230684 1106 54-55 -0.3705959516940567 1106 56-57 -0.03470209417463366 1106 58-59 -0.1570105589946067 1106 60-61 -0.3203232257812303 1106 62-63 -0.14255229714807172 1106 64-65 -0.3261786501156081 1106 66-67 -0.9891213471650175 1106 68-69 -0.8599711350268713 1106 70-71 -0.393037716836556 1106 72-73 0.01841529219584359 1106 74-75 0.09120455915583037 1106 76-77 -0.10887546557155403 1106 78-79 -0.4513475715257407 1106 80-81 -0.47245409510698266 1106 82-83 -0.4908788944067446 1106 84-85 -0.3372058032802272 1106 86-87 -0.18199543834549203 1106 88-89 -0.044021186212997776 1106 90-91 0.1231983548601896 1106 92-93 -0.0876227543385859 1106 94-95 0.4437397897043738 1106 96-97 0.4046134221394375 1106 98-99 0.6754787051382714 1106 100-101 0.39178242362063287 1107 1 0.16125240509892436 1107 2 0.1485102970066272 1107 3 0.09399559207890462 1107 4 -0.0803715086689678 1107 5 0.014136039612942852 1107 6 0.11825001984695405 1107 7 0.21459632334339318 1107 8 0.03200778857251407 1107 9 0.09298160890207896 1107 10-11 0.12991536040998142 1107 12-13 0.13894009659194495 1107 14-15 0.27068604138035823 1107 16-17 0.10491557877632118 1107 18-19 0.18789362111905206 1107 20-21 0.002620974108985763 1107 22-23 0.13380714112885528 1107 24-25 0.23698205105954884 1107 26-27 0.313680379990835 1107 28-29 0.1506380279263837 1107 30-31 0.263986015876462 1107 32-33 0.6379534063295225 1107 34-35 0.4635969980857553 1107 36-37 0.5702231079383537 1107 38-39 0.5045552895964747 1107 40-41 0.46914872885981396 1107 42-43 0.6352398156236774 1107 44-45 0.4134352201559608 1107 46-47 0.4352571965563712 1107 48-49 0.46329426998113377 1107 50-51 0.3766685562320262 1107 52-53 0.4359818660033099 1107 54-55 0.6565144920830548 1107 56-57 0.985868476395936 1107 58-59 0.8446077259570188 1107 60-61 0.8262900541653835 1107 62-63 0.6186755974131586 1107 64-65 0.7406715334012475 1107 66-67 0.7271616024512682 1107 68-69 0.5945333694776309 1107 70-71 0.687426080293907 1107 72-73 1.0471106542245394 1107 74-75 0.7787754633933979 1107 76-77 0.794544621181867 1107 78-79 1.1715419846971464 1107 80-81 1.0273623880428318 1107 82-83 1.0180800645522154 1107 84-85 0.7213360719283166 1107 86-87 1.1556255159421305 1107 88-89 1.0851079429161317 1107 90-91 1.0542127025412036 1107 92-93 0.9155137154645523 1107 94-95 1.5632926759239254 1107 96-97 1.3480902322829138 1107 98-99 1.165602161928394 1107 100-101 1.5086159237874668 1108 1 0.21265159189634275 1108 2 0.15841223434219032 1108 3 0.05279332786778923 1108 4 0.14591406894404457 1108 5 0.24286122497529306 1108 6 0.1600103642621704 1108 7 0.024306918894701823 1108 8 0.11497039719883873 1108 9 0.006638628753790954 1108 10-11 0.10820604047057003 1108 12-13 0.07208607442818504 1108 14-15 0.20794188129107027 1108 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sequence quality scores pass #Quality Count 3 2.0 4 1.0 5 7.0 6 17.0 7 52.0 8 116.0 9 249.0 10 376.0 11 595.0 12 954.0 13 1296.0 14 1716.0 15 2569.0 16 3435.0 17 4900.0 18 4030.0 19 2480.0 20 2180.0 21 2326.0 22 2554.0 23 2786.0 24 3261.0 25 3658.0 26 4368.0 27 5086.0 28 6140.0 29 7433.0 30 9243.0 31 11055.0 32 13731.0 33 17717.0 34 23684.0 35 32370.0 36 46616.0 37 66622.0 38 39457.0 39 1427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.30067402017438 24.0422102437506 17.074869556909555 18.582246179165473 2 14.476571456748728 26.027502804521752 39.7117198190313 19.784205919698223 3 18.24614170898871 30.72950051772595 32.642621172526006 18.381736600759332 4 11.152267432544036 21.149247476179323 43.44823548263833 24.250249608638306 5 9.906281107368383 41.74173526338984 37.03390953553521 11.318074093706565 6 24.686834571614348 43.704488935591925 18.270679703798663 13.337996788995065 7 23.53925468939228 36.519788357179614 25.242751356664993 14.698205596763108 8 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33.947495739691284 17.138305182939042 32-33 18.108040228105125 31.50266795480424 33.80606133445506 16.583230482635567 34-35 19.092837893848046 29.761499384449923 34.519801606439316 16.625861115262712 36-37 19.301029873099417 29.299458253468348 34.5067579644136 16.89275390901863 38-39 17.95154286841319 30.987571380584484 34.744817299602765 16.316068451399566 40-41 17.746811336511467 30.69776184944023 34.86667550052541 16.688751313522893 42-43 17.750176424725964 31.162307666232152 32.95629289277999 18.13122301626189 44-45 18.615584459610428 30.5690428018682 32.94882482468292 17.866547913838453 46-47 19.381126563515956 30.970173400429573 32.92712991011035 16.72157012594412 48-49 19.02048551991926 31.106865230086516 32.45587408223484 17.416775167759383 50-51 18.17838636563242 31.296734858021562 32.73200443138518 17.79287434496084 52-53 18.87188700380424 31.94419834425505 31.88241221670444 17.301502435236273 54-55 18.831372935727515 30.574960940990852 32.1196022298303 18.474063893451337 56-57 19.701966344079395 30.910744005424394 31.189360784071994 18.197928866424213 58-59 19.923728813559322 29.919106317411405 31.994298921417563 18.16286594761171 60-61 19.241987898062675 31.675673010399287 31.66272976192127 17.419609329616772 62-63 18.864462091726836 30.969747956130377 32.90422086426487 17.26156908787792 64-65 18.565868300379666 31.306549233272523 32.05124254228095 18.07633992406686 66-67 18.6674987095631 30.748915647799326 31.300221107695624 19.28336453494195 68-69 18.051799771648337 31.01715102796769 32.22901730202974 18.702031898354235 70-71 19.62821601315297 30.605612816439358 32.143875225546594 17.622295944861083 72-73 19.13145576076204 29.587484630058213 32.392749393676986 18.888310215502763 74-75 18.493132742326438 31.815134933561808 31.137423742081427 18.55430858203033 76-77 18.946203887175155 30.294172935227103 31.752865150707386 19.006758026890353 78-79 18.436056348445675 29.801128571472596 32.02036482875731 19.742450251324424 80-81 19.505855161787366 31.106471494607085 30.494144838212634 18.89352850539291 82-83 19.684028703371276 30.076904942040084 31.424089691101308 18.814976663487336 84-85 18.057849711552684 30.43781741531483 31.30839825452394 20.19593461860855 86-87 18.357036101138657 30.59922035099613 30.856073095946137 20.187670451919075 88-89 19.083506036256058 29.749843989553078 30.672039075801816 20.49461089838905 90-91 18.59828306471869 28.266561059685923 32.57199740502691 20.563158470568474 92-93 19.574569716020278 30.27535092990647 31.160572582012048 18.9895067720612 94-95 18.875922170930583 29.09977011210887 31.57460878997615 20.4496989269844 96-97 18.423890936433 28.92625143293848 31.77810716530871 20.871750465319806 98-99 18.298233611912334 30.17235534847028 30.66295022557503 20.866460814042355 100-101 17.833672574757664 29.686577904058108 30.80029645745666 21.679453063727568 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 892.0 1 588.0 2 317.0 3 343.5 4 314.0 5 338.5 6 505.5 7 849.5 8 1196.5 9 1374.5 10 1272.0 11 1056.5 12 1010.5 13 1037.0 14 996.0 15 924.0 16 888.5 17 864.5 18 851.0 19 848.0 20 878.0 21 951.5 22 1049.0 23 1153.0 24 1224.5 25 1490.5 26 2078.0 27 2742.0 28 3083.0 29 3706.5 30 5506.5 31 6907.5 32 9449.0 33 13286.0 34 15955.0 35 18088.5 36 21003.5 37 22531.0 38 21137.5 39 19222.5 40 16820.5 41 14283.5 42 11527.0 43 10569.0 44 10893.0 45 10021.5 46 8471.0 47 9384.5 48 15028.0 49 13447.0 50 6000.0 51 2650.0 52 1280.5 53 948.5 54 766.5 55 647.0 56 587.0 57 534.5 58 531.0 59 517.0 60 446.0 61 402.5 62 366.5 63 258.5 64 160.5 65 120.5 66 75.5 67 58.0 68 57.0 69 50.5 70 37.0 71 24.0 72 19.5 73 12.0 74 8.0 75 7.0 76 5.5 77 5.0 78 7.0 79 8.5 80 5.0 81 1.5 82 0.5 83 0.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011709998798184334 2 0.010169209482633763 3 0.0040060522204314825 4 3.08157863110114E-4 5 0.008628420167083194 6 0.0 7 0.0 8 0.0021571050417707985 9 0.0 10-11 0.0 12-13 0.0 14-15 6.16315726220228E-4 16-17 0.004622367946651711 18-19 0.0029274996995460835 20-21 7.703946577752851E-4 22-23 3.08157863110114E-4 24-25 4.622367946651711E-4 26-27 3.08157863110114E-4 28-29 1.54078931555057E-4 30-31 6.16315726220228E-4 32-33 0.0029274996995460835 34-35 7.703946577752851E-4 36-37 9.244735893303422E-4 38-39 0.004930525809761824 40-41 0.0 42-43 0.002465262904880912 44-45 0.00785802550930791 46-47 0.0 48-49 0.0035438154257663116 50-51 0.0016948682471056272 52-53 7.703946577752851E-4 54-55 0.0 56-57 0.01509973529239559 58-59 0.0027734207679910266 60-61 0.004776446878206768 62-63 6.16315726220228E-4 64-65 0.0040060522204314825 66-67 0.0020030261102157412 68-69 0.0029274996995460835 70-71 0.006009078330647224 72-73 0.003697894357321369 74-75 0.010631446277298935 76-77 0.0018489471786606844 78-79 0.008628420167083194 80-81 0.0027734207679910266 82-83 0.005392762604426996 84-85 0.0040060522204314825 86-87 0.001232631452440456 88-89 0.0035438154257663116 90-91 0.01093960414040905 92-93 0.004314210083541597 94-95 9.244735893303422E-4 96-97 3.08157863110114E-4 98-99 0.0015407893155505701 100-101 0.003235657562656197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 324509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.961569632891536 #Duplication Level Percentage of deduplicated Percentage of total 1 70.48690112461809 13.365422838811867 2 9.61288435285705 3.6455075205926493 3 4.269323278944289 2.428592119170809 4 2.333745043229539 1.770058765704495 5 1.6999284924917115 1.6116656240658964 6 1.1863745693297796 1.3497314404222995 7 1.0271078463238639 1.3632903863991446 8 0.8369628811025157 1.26961039601367 9 0.5655593837352921 0.9651504272608772 >10 6.203276344016122 25.21131925462776 >50 1.0514854059676266 14.042445664064788 >100 0.6825716700253527 23.040655266880115 >500 0.026002730286680098 3.4738635908403155 >1k 0.017876877072092567 6.4626867051453125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3940 1.2141419806538494 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3318 1.0224677897993584 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2986 0.9201593792468006 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2015 0.6209380941668798 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1821 0.5611554687235177 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1344 0.41416416801999323 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1288 0.3969073276858269 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1127 0.34729391172509855 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1055 0.3251065455811703 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1041 0.3207923354976287 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1037 0.3195597040451883 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 979 0.30168654798480166 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 820 0.2526894477502935 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 817 0.25176497416096316 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 796 0.24529365903565079 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 771 0.23758971245789792 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 759 0.23389181810057658 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 734 0.2261878715228237 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 732 0.22557155579660346 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 729 0.22464708220727314 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 680 0.20954734691487753 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 659 0.20307603178956515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.1975291902535831 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 591 0.1821212970980774 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 531 0.16363182531147055 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 529 0.16301550958525032 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 505 0.1556197208706076 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 499 0.15377077369194692 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 496 0.15284630010261657 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 493 0.15192182651328623 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 479 0.14760761642974463 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 472 0.14545051138797382 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 466 0.14360156420931314 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 449 0.13836288053644122 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 444 0.13682209122089065 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 433 0.1334323547266794 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 427 0.1315834075480187 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 421 0.129734460369358 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 417 0.12850182891691755 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 414 0.1275773553275872 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.1272691974644771 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 389 0.11987340874983435 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 382 0.11771630370806356 No Hit TTCTAGAATTGTGAGTAGAAGTAAAATAATAAATGTAATGGTAGCTGTTG 374 0.11525104080318264 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 364 0.11216946217208151 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 360 0.11093683071964106 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 354 0.10908788354098038 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 354 0.10908788354098038 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 352 0.10847156781476014 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 349 0.10754709422542981 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 348 0.10723893636231968 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 343 0.10569814704676911 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 330 0.10169209482633763 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 329 0.10138393696322752 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 328 0.1010757791001174 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 325 0.10015130551078706 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.5407893155505704E-4 0.0 18-19 0.0 0.0 0.0 3.0815786311011407E-4 0.0 20-21 0.0 0.0 0.0 4.622367946651711E-4 0.0 22-23 0.0 0.0 0.0 0.0016948682471056274 0.0 24-25 0.0 0.0 0.0 0.0036978943573213684 0.0 26-27 0.0 0.0 0.0 0.006779472988422509 0.0 28-29 0.0 0.0 0.0 0.011709998798184334 0.0 30-31 0.0 0.0 0.0 0.021108813623042812 0.0 32-33 0.0 0.0 0.0 0.03050762844790129 0.0 34-35 0.0 0.0 0.0 0.03882789075187437 0.0 36-37 0.0 0.0 0.0 0.04699407412429239 0.0 38-39 0.0 0.0 0.0 0.06163157262202281 0.0 40-41 0.0 0.0 0.0 0.07334157142020714 0.0 42-43 0.0 0.0 0.0 0.10893380460942531 0.0 44-45 0.0 0.0 0.0 0.14976472147151543 0.0 46-47 0.0 0.0 0.0 0.1853569546607336 0.0 48-49 0.0 0.0 0.0 0.22665010831748889 0.0 50-51 0.0 0.0 0.0 0.2802695764986487 0.0 52-53 0.0 0.0 0.0 0.3989103537960426 0.0 54-55 0.0 0.0 0.0 0.4927444231130723 0.0 56-57 0.0 0.0 0.0 0.6576088798769834 0.0 58-59 0.0 0.0 0.0 0.8585278066247777 0.0 60-61 0.0 0.0 0.0 1.0438847612855113 0.0 62-63 0.0 0.0 0.0 1.2965742090358048 0.0 64-65 0.0 0.0 0.0 1.4434114308077741 0.0 66-67 0.0 0.0 0.0 1.76990468677294 0.0 68-69 0.0 0.0 0.0 2.156950962839243 0.0 70-71 0.0 0.0 0.0 2.387453044445609 0.0 72-73 0.0 0.0 0.0 2.8324329987766133 0.0 74-75 0.0 0.0 0.0 3.301603345361762 0.0 76-77 0.0 0.0 0.0 3.559377397853372 0.0 78-79 0.0 0.0 0.0 3.820078950044529 0.0 80-81 0.0 0.0 0.0 4.346566659168159 0.0 82-83 0.0 0.0 0.0 4.7710541156023405 0.0 84-85 0.0 0.0 0.0 5.411097997282047 0.0 86-87 0.0 0.0 0.0 6.291967248982308 0.0 88-89 0.0 0.0 0.0 7.4740608118727065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTGAC 25 0.0050100167 57.023033 2 GACCGTG 25 0.005019197 56.99667 6 CCGTGCA 25 0.005019197 56.99667 8 ACCGTGC 25 0.005019197 56.99667 7 CGTGCAA 25 0.00502226 56.987892 9 GTATGGT 70 7.8627636E-8 47.526524 1 GTAAGCG 20 5.3920824E-4 47.489906 94-95 TTGCAGA 30 8.282086E-5 39.58712 84-85 GGATGCC 60 8.2804414E-5 39.581024 8 GGGATGC 60 8.2804414E-5 39.581024 7 CATATAG 75 6.8951085E-6 38.009495 3 TATAGGG 115 2.4811015E-9 37.188934 2 AGGGGTC 90 5.6769204E-7 36.94798 5 AGCAGAT 135 3.0559022E-10 35.199406 2 GGTACTC 125 5.627953E-9 34.198006 8 CAGACGC 35 2.051196E-4 33.93182 88-89 ACCCTTG 155 3.6379788E-11 33.707706 7 AGATCAG 130 8.240022E-9 32.887764 5 GATCAGT 130 8.252755E-9 32.882698 6 GCAGATC 145 6.5847416E-10 32.76681 3 >>END_MODULE