##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356698 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.649953181683106 31.0 31.0 34.0 30.0 34.0 2 31.728128556930514 33.0 31.0 34.0 30.0 34.0 3 31.73565873652221 33.0 31.0 34.0 30.0 34.0 4 35.40562044082109 37.0 35.0 37.0 33.0 37.0 5 35.363212016888234 37.0 35.0 37.0 33.0 37.0 6 35.48108203578377 37.0 35.0 37.0 33.0 37.0 7 35.360736533426035 37.0 35.0 37.0 33.0 37.0 8 35.34985618085888 37.0 35.0 37.0 33.0 37.0 9 36.9091360198263 39.0 37.0 39.0 33.0 39.0 10-11 36.7997633852727 39.0 37.0 39.0 33.0 39.0 12-13 36.76981087642768 39.0 37.0 39.0 32.5 39.0 14-15 37.837972178145094 40.0 37.0 41.0 33.0 41.0 16-17 37.80024698764781 40.0 37.0 41.0 33.0 41.0 18-19 37.878301812737945 40.0 37.5 41.0 33.0 41.0 20-21 37.81055682958693 40.0 37.0 41.0 32.5 41.0 22-23 37.72207301414642 40.0 37.0 41.0 32.0 41.0 24-25 37.600394451328576 40.0 37.0 41.0 32.0 41.0 26-27 37.41018592759141 39.0 36.5 41.0 32.0 41.0 28-29 37.5161943717094 39.0 37.0 41.0 32.0 41.0 30-31 37.46469702661635 39.0 36.5 41.0 32.0 41.0 32-33 37.25847635815171 39.0 36.0 41.0 32.0 41.0 34-35 37.09270166919916 39.0 35.5 41.0 31.5 41.0 36-37 36.86099865993081 39.0 35.0 41.0 31.0 41.0 38-39 36.68896237152998 39.0 35.0 41.0 31.0 41.0 40-41 36.62970075526076 39.0 35.0 41.0 31.0 41.0 42-43 36.37189723519616 39.0 35.0 40.0 30.0 41.0 44-45 36.08665173339912 39.0 35.0 40.0 30.0 41.0 46-47 35.71802477165558 38.0 35.0 40.0 28.5 41.0 48-49 35.43334978048657 38.5 35.0 40.0 27.0 41.0 50-51 34.684199238571566 38.0 34.0 39.5 26.0 40.5 52-53 34.91225630645532 38.0 34.5 40.0 27.0 40.5 54-55 35.33095784108686 39.0 35.0 40.0 27.0 41.0 56-57 35.284826379738604 39.0 35.0 41.0 26.5 41.0 58-59 35.09839836500345 39.0 35.0 41.0 26.0 41.0 60-61 34.79454468485946 38.5 35.0 40.0 25.5 41.0 62-63 34.50122232252494 38.0 34.0 40.0 24.0 41.0 64-65 34.20471939848275 38.0 34.0 40.0 23.0 41.0 66-67 33.817966459021356 37.5 34.0 40.0 22.0 41.0 68-69 33.421590533168114 37.0 33.5 39.0 21.5 41.0 70-71 32.96641977246858 36.5 33.0 39.0 19.5 41.0 72-73 32.493315073255246 36.0 32.5 39.0 18.0 40.0 74-75 32.03809665319122 35.5 32.0 38.0 14.5 40.0 76-77 31.577177332084844 35.0 31.5 37.0 8.0 39.0 78-79 31.06901356329444 35.0 31.0 37.0 4.5 39.0 80-81 30.5833239883599 35.0 31.0 36.0 2.0 38.5 82-83 30.111768218492955 35.0 30.5 36.0 2.0 37.0 84-85 29.66110547297714 34.0 30.0 35.5 2.0 37.0 86-87 29.362836348956257 34.0 30.0 35.0 2.0 36.5 88-89 28.943097522273746 34.0 29.0 35.0 2.0 36.0 90-91 28.57053025248249 34.0 29.0 35.0 2.0 36.0 92-93 28.21498858978744 34.0 29.0 35.0 2.0 35.5 94-95 27.818437445682342 34.0 29.0 35.0 2.0 35.0 96-97 27.28358723626149 34.0 27.0 35.0 2.0 35.0 98-99 26.767968982164184 34.0 25.5 35.0 2.0 35.0 100-101 24.791666900291006 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3468203762508111 1101 2 0.3329940524070345 1101 3 0.3694251344623858 1101 4 0.39003770347058975 1101 5 0.3795106375443069 1101 6 0.2701540879817088 1101 7 0.20987392747442613 1101 8 0.235627085820461 1101 9 0.37447355466456145 1101 10-11 0.36718877435951214 1101 12-13 0.3065064242801796 1101 14-15 0.549086083364017 1101 16-17 0.3388181151571814 1101 18-19 0.699509235323795 1101 20-21 0.631543669883122 1101 22-23 0.7639814323037228 1101 24-25 0.6886480526661884 1101 26-27 0.696378761914012 1101 28-29 0.5737263871266478 1101 30-31 0.4946299906422098 1101 32-33 0.5537183179514287 1101 34-35 0.5322434053647527 1101 36-37 0.827229772085758 1101 38-39 0.9318469428627552 1101 40-41 0.8197754344665427 1101 42-43 0.5947081321723502 1101 44-45 0.5484404430496355 1101 46-47 0.9388492280222849 1101 48-49 0.7036468434838028 1101 50-51 0.5916233957860868 1101 52-53 0.7103064231056351 1101 54-55 0.7984199214603436 1101 56-57 0.6633288386912071 1101 58-59 0.6542402290272022 1101 60-61 1.0583423924061606 1101 62-63 1.0130828951000623 1101 64-65 1.1336660610249396 1101 66-67 0.9298745658778031 1101 68-69 0.8101450300842572 1101 70-71 0.6572107927931654 1101 72-73 0.7646042168222635 1101 74-75 0.6594307329795548 1101 76-77 0.7770357561421832 1101 78-79 0.7579314294301547 1101 80-81 0.5861695722859572 1101 82-83 0.7152398568617215 1101 84-85 0.8345079603498853 1101 86-87 0.9381851111543398 1101 88-89 0.7618184146305573 1101 90-91 1.1061289203850535 1101 92-93 0.6919588397524343 1101 94-95 0.684292802563867 1101 96-97 0.6945207359318566 1101 98-99 0.32124725307144075 1101 100-101 0.46029300204611445 1102 1 0.10362068074205766 1102 2 0.1955416291642038 1102 3 0.3464772785882424 1102 4 0.20291514574412162 1102 5 0.14907420009188144 1102 6 0.1505651511636401 1102 7 0.07679539410725766 1102 8 0.11351023730485821 1102 9 0.20942978404796975 1102 10-11 0.5167526540854652 1102 12-13 0.4349731839858393 1102 14-15 0.48273236603338887 1102 16-17 0.39434653434013 1102 18-19 0.38684747434942324 1102 20-21 0.33618716138273896 1102 22-23 -0.09908473110657923 1102 24-25 0.21107097831957589 1102 26-27 0.17312165711827987 1102 28-29 0.04680270024514499 1102 30-31 0.15183311167748315 1102 32-33 0.2681150446705374 1102 34-35 0.4045695916118959 1102 36-37 0.283679594465859 1102 38-39 0.027434991581351653 1102 40-41 0.03390731723741425 1102 42-43 0.061670831994725006 1102 44-45 0.03603083888825154 1102 46-47 0.7031920344166096 1102 48-49 0.8642136412508634 1102 50-51 0.6904038449106693 1102 52-53 0.6466471361226382 1102 54-55 0.6902700229574137 1102 56-57 0.948001193423508 1102 58-59 0.6636588410546054 1102 60-61 0.42256784279945947 1102 62-63 0.641628949401003 1102 64-65 0.7515200320982771 1102 66-67 0.8096195544593812 1102 68-69 0.9756328504344154 1102 70-71 1.0642394657188134 1102 72-73 0.8098512987811546 1102 74-75 0.6945376220939927 1102 76-77 0.7484801864898074 1102 78-79 0.4220046342005155 1102 80-81 0.8269602979900945 1102 82-83 1.0489321303963557 1102 84-85 0.6387422917378593 1102 86-87 0.6358586077288138 1102 88-89 0.3519922283834127 1102 90-91 0.18879829364056988 1102 92-93 0.43129371668721816 1102 94-95 0.7689699149718834 1102 96-97 0.6407098376826816 1102 98-99 0.7146166390140962 1102 100-101 1.0794664351849121 1103 1 0.20966664574597615 1103 2 0.34422274694953003 1103 3 0.2191431182330561 1103 4 0.049624469327305576 1103 5 0.5247205829594748 1103 6 0.14918097775021977 1103 7 0.3266419597902015 1103 8 0.2970731780204474 1103 9 -0.033078050895881006 1103 10-11 0.20423469900580926 1103 12-13 0.21909762901064767 1103 14-15 0.1816093574149491 1103 16-17 0.3379251449434193 1103 18-19 0.2333609243363668 1103 20-21 -0.003065907303600568 1103 22-23 0.19142029631571944 1103 24-25 0.09261478871393791 1103 26-27 0.21442650172522804 1103 28-29 0.208256408785239 1103 30-31 0.14364885991462018 1103 32-33 0.19539697206672457 1103 34-35 0.07157468432540526 1103 36-37 0.13179431110205542 1103 38-39 0.24748918327630776 1103 40-41 0.1979726737273637 1103 42-43 0.26989752303677506 1103 44-45 0.5516762423878632 1103 46-47 0.44830172363050025 1103 48-49 0.40302436206570036 1103 50-51 -0.2115010258127512 1103 52-53 0.2536414557883404 1103 54-55 0.6277781843572612 1103 56-57 0.5351535177901781 1103 58-59 0.469530152101882 1103 60-61 0.8928701410496558 1103 62-63 0.7585263116365795 1103 64-65 0.6787431974155922 1103 66-67 0.5557216698942256 1103 68-69 0.4407183819047731 1103 70-71 0.8796220721434622 1103 72-73 0.7340824949159099 1103 74-75 0.9114464102893471 1103 76-77 0.45475793524927255 1103 78-79 0.5166736078228702 1103 80-81 0.799444954558389 1103 82-83 0.6804335015115299 1103 84-85 0.41521460826959 1103 86-87 0.5040928072587185 1103 88-89 0.6639862215931274 1103 90-91 0.9414229998301771 1103 92-93 0.8945429331928345 1103 94-95 0.7252476637313521 1103 96-97 0.9433655571916901 1103 98-99 0.9288582817406592 1103 100-101 0.8748536119934798 1104 1 0.05651252573916565 1104 2 0.11358674194616114 1104 3 0.19216119993005876 1104 4 0.013239032383800975 1104 5 0.04748368534347236 1104 6 0.06103363005317419 1104 7 0.1510652032454871 1104 8 0.14075864679011119 1104 9 0.3790003450658972 1104 10-11 0.14934013239662391 1104 12-13 0.4252348959580843 1104 14-15 0.3959770161201419 1104 16-17 0.1631218379838586 1104 18-19 0.271202592915067 1104 20-21 0.2961351081727912 1104 22-23 0.2886936089743912 1104 24-25 0.32590975796099286 1104 26-27 0.3622663939603896 1104 28-29 0.37174847815145284 1104 30-31 0.32616075771565534 1104 32-33 0.15479086884635507 1104 34-35 0.223662998205306 1104 36-37 0.5409417402148478 1104 38-39 0.8218754195744111 1104 40-41 0.47062290428194586 1104 42-43 0.4823186052740809 1104 44-45 0.15201189730351672 1104 46-47 0.5739410222708514 1104 48-49 0.8131617326331693 1104 50-51 0.5608383446214873 1104 52-53 0.9017403981237919 1104 54-55 1.0079934179834495 1104 56-57 1.1280655369746952 1104 58-59 1.3583125426162113 1104 60-61 1.4140585888954647 1104 62-63 1.30391929063871 1104 64-65 1.3390489505482464 1104 66-67 1.0981244705958773 1104 68-69 1.1370339493355175 1104 70-71 1.2848004836482545 1104 72-73 1.008090888139165 1104 74-75 0.5990597447082244 1104 76-77 1.0942508631815357 1104 78-79 1.1673704525926532 1104 80-81 1.5069884934788114 1104 82-83 1.3345849644044527 1104 84-85 1.271832228117269 1104 86-87 1.375502187832918 1104 88-89 1.1037996315066074 1104 90-91 0.8050793809742878 1104 92-93 0.6001232424246403 1104 94-95 0.8525948426757566 1104 96-97 1.071880311945261 1104 98-99 1.184805331900158 1104 100-101 1.325584133276294 1105 1 0.17881605429629488 1105 2 0.3372421605802707 1105 3 0.26279878530909073 1105 4 0.24591295756031428 1105 5 0.33158435951670384 1105 6 0.19079647345185435 1105 7 0.2977355316502397 1105 8 0.1700865111404326 1105 9 0.21109045925896908 1105 10-11 0.3508590154483926 1105 12-13 0.24617590358571562 1105 14-15 0.33409216597086555 1105 16-17 0.21713099823509907 1105 18-19 0.23189280101400556 1105 20-21 -0.012046661686966331 1105 22-23 -0.1872275101000156 1105 24-25 -0.04838705544097621 1105 26-27 -0.14464366016837005 1105 28-29 -0.14559531475954657 1105 30-31 0.19162288973312513 1105 32-33 0.2755197023760658 1105 34-35 0.09809491202452847 1105 36-37 0.06673484610699632 1105 38-39 0.16934261896491165 1105 40-41 0.10049648572756809 1105 42-43 0.22228352346080982 1105 44-45 0.418875087090818 1105 46-47 0.8308178549855327 1105 48-49 0.47844269720585686 1105 50-51 0.43561133162527454 1105 52-53 0.8355323346221653 1105 54-55 0.6906314640327551 1105 56-57 0.9245622281218502 1105 58-59 0.7531379751590137 1105 60-61 0.7213057296390986 1105 62-63 0.766542985037141 1105 64-65 0.8308299727047128 1105 66-67 0.7625970474259276 1105 68-69 1.0828343196435455 1105 70-71 1.2737111756281578 1105 72-73 0.8575048623947836 1105 74-75 1.0011119421940258 1105 76-77 1.3678026310037126 1105 78-79 1.0838872537691309 1105 80-81 1.172484289862556 1105 82-83 1.504060686195043 1105 84-85 1.2005545768269492 1105 86-87 0.991721768091427 1105 88-89 1.1073454637694695 1105 90-91 1.0333747237749868 1105 92-93 1.0522201780814058 1105 94-95 1.1612682894639121 1105 96-97 1.4343347986079706 1105 98-99 1.2796037443595196 1105 100-101 0.7959129809804715 1106 1 -0.052419359977317725 1106 2 0.01769975607292551 1106 3 -9.735188328647837E-4 1106 4 0.011584979473340695 1106 5 0.4449852961217431 1106 6 0.2798831016217349 1106 7 0.4185794117523187 1106 8 0.26988536455290557 1106 9 0.21352292961973518 1106 10-11 0.30330889772306335 1106 12-13 0.3557358239586961 1106 14-15 0.3503943952156092 1106 16-17 0.13582515666792716 1106 18-19 0.1762604366840037 1106 20-21 0.261195644585996 1106 22-23 0.37204056580553413 1106 24-25 0.21799963458018112 1106 26-27 0.16042866424417213 1106 28-29 0.1398613191796514 1106 30-31 0.4255818391969939 1106 32-33 0.013597738935892778 1106 34-35 0.2878873724059261 1106 36-37 0.5630887393589745 1106 38-39 0.5871609841161316 1106 40-41 0.6386727566673471 1106 42-43 0.6203406657239441 1106 44-45 0.37598460543049583 1106 46-47 0.5267668562693402 1106 48-49 0.300061537416461 1106 50-51 0.8220440722605176 1106 52-53 0.4203725348331915 1106 54-55 0.8268601994080456 1106 56-57 0.6357323607122751 1106 58-59 0.8157351050518926 1106 60-61 0.7651577647539867 1106 62-63 0.5621583012842777 1106 64-65 0.4379002492682744 1106 66-67 0.7135812103806529 1106 68-69 0.8331786597320132 1106 70-71 0.6001403819047653 1106 72-73 0.7005091711937723 1106 74-75 0.4434369264713318 1106 76-77 0.7214230096998477 1106 78-79 0.8387716854390632 1106 80-81 0.5934367995170682 1106 82-83 0.7138222729141717 1106 84-85 0.7471873205807498 1106 86-87 0.9330358218448644 1106 88-89 1.2496423810810704 1106 90-91 1.1436337194973767 1106 92-93 1.1621959053536592 1106 94-95 1.149869982837508 1106 96-97 1.0246532547169522 1106 98-99 0.9588035239818637 1106 100-101 1.1769766150288206 1107 1 0.4071664155827577 1107 2 0.20181089946225228 1107 3 0.416813866460803 1107 4 0.4910446421470027 1107 5 0.45066721139712485 1107 6 0.10061653862509701 1107 7 0.3653488370671738 1107 8 0.44564024758235377 1107 9 0.024370688467293178 1107 10-11 0.3089096427802218 1107 12-13 0.502303605363025 1107 14-15 0.5202452128987503 1107 16-17 0.35360912426818913 1107 18-19 0.47071181145116725 1107 20-21 0.45720463311963755 1107 22-23 0.727578937958171 1107 24-25 0.6091503719475284 1107 26-27 0.6036522326578435 1107 28-29 0.4160280342636469 1107 30-31 0.5924934405572344 1107 32-33 0.5174084670754695 1107 34-35 0.5961035073364584 1107 36-37 0.41582887504139876 1107 38-39 0.7602073451270854 1107 40-41 0.35328681364029535 1107 42-43 0.7832535658076765 1107 44-45 0.4506313013754024 1107 46-47 0.6296308833374695 1107 48-49 0.650242675166588 1107 50-51 0.2557237318582608 1107 52-53 0.38234215153887874 1107 54-55 0.46901488421126913 1107 56-57 0.34163557163522285 1107 58-59 0.41348540837218195 1107 60-61 0.2752271439299889 1107 62-63 0.42909040880491034 1107 64-65 0.3416921800941637 1107 66-67 0.07351986278415268 1107 68-69 -0.08571093168274757 1107 70-71 0.07241007209086092 1107 72-73 0.15181558449543786 1107 74-75 -0.2625602710200887 1107 76-77 -0.3674113859665127 1107 78-79 -0.3672682332198356 1107 80-81 -0.016108818145568193 1107 82-83 0.03872529586397988 1107 84-85 -0.10877826324672313 1107 86-87 0.21059524698883436 1107 88-89 0.6134259385581302 1107 90-91 1.0090449534555752 1107 92-93 0.489136727992463 1107 94-95 1.016387695033547 1107 96-97 0.7206366105501019 1107 98-99 0.6890578003707262 1107 100-101 1.151036322132498 1108 1 0.008770182403186055 1108 2 0.021787476340286105 1108 3 7.546872261166016E-4 1108 4 0.015041391591303466 1108 5 0.13296124328125103 1108 6 -0.05109597840446867 1108 7 0.20956117483815717 1108 8 -0.2726608590406556 1108 9 0.33546799002803596 1108 10-11 0.1556853783379566 1108 12-13 0.1084366391353413 1108 14-15 0.014293827827408734 1108 16-17 0.10917211573548258 1108 18-19 0.1350202318245053 1108 20-21 0.05877852350899104 1108 22-23 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2316 76-77 -2.945396522609677 2316 78-79 -2.431773064997973 2316 80-81 -2.8399804139382425 2316 82-83 -2.965073645181203 2316 84-85 -3.112666241972658 2316 86-87 -3.505422038192922 2316 88-89 -3.5673264892026317 2316 90-91 -3.5416590378068555 2316 92-93 -4.190155481804059 2316 94-95 -4.141903493797564 2316 96-97 -4.183220501779836 2316 98-99 -4.348628881966576 2316 100-101 -5.422378213565679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 517.0 3 158.0 4 157.0 5 156.0 6 167.0 7 209.0 8 237.0 9 321.0 10 442.0 11 639.0 12 1023.0 13 1398.0 14 1692.0 15 2287.0 16 3188.0 17 4139.0 18 3638.0 19 2096.0 20 2247.0 21 2414.0 22 2632.0 23 2903.0 24 3269.0 25 3701.0 26 4298.0 27 4822.0 28 5734.0 29 6732.0 30 8414.0 31 10422.0 32 12921.0 33 16706.0 34 22361.0 35 31690.0 36 43663.0 37 66240.0 38 70120.0 39 12942.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.148844955741296 24.320558077459044 16.28613806697266 21.244458899827006 2 13.193671478757734 24.652971505953666 39.909608574849095 22.24374844043951 3 16.279382738202045 30.62982235779652 32.60054055266463 20.490254351336805 4 9.660411858241792 20.561782942910266 43.26867258026973 26.509132618578214 5 9.217811285192603 41.200096483404295 37.49081444334124 12.091277788061863 6 22.53434206576958 43.71940631776194 19.403232095420027 14.343019521048452 7 21.886313097464743 36.29360844169137 26.002563016844686 15.817515443999202 8 21.501360029163514 35.27214604189451 23.352401783460923 19.874092145481058 9 23.314257836047926 18.856703855007527 24.905440301918112 32.92359800702644 10-11 20.156251972081634 29.14235309131246 30.736005407535487 19.965389529070414 12-13 22.570765466031876 28.942847727161176 29.63237717274697 18.854009634059977 14-15 20.624349810866814 30.431655483179902 28.930787629652272 20.013207076301008 16-17 16.364569371147837 33.226309384271126 30.507013106461255 19.902108138119782 18-19 15.970459816208121 33.003317451815626 33.24013808227168 17.78608464970457 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31.377306783854443 32.16439930752679 19.732823089916383 46-47 17.753726290586886 31.436673736327354 31.999489750861045 18.81011022222471 48-49 17.333972533996082 31.386014121288326 32.015542668746875 19.264470675968717 50-51 16.691781810841615 31.593075789795357 32.20607221106253 19.5090701883005 52-53 17.034277135539977 32.59572941065979 31.219765878739707 19.150227575060523 54-55 17.148972914780103 31.095856614585816 31.83024988154947 19.924920589084607 56-57 17.81452906148722 31.551730905094594 30.62502102654391 20.00871900687428 58-59 17.821572604667658 30.73746420195773 31.49148346526451 19.949479728110102 60-61 17.87801903587098 32.09716985099315 31.272235383170493 18.752575729965375 62-63 17.479425786347775 31.93681463092573 31.962326515679294 18.621433067047196 64-65 17.440245145246596 31.381479254191003 31.83776229265756 19.340513307904843 66-67 17.203313801900812 31.207070565477025 30.86587793321932 20.723737699402843 68-69 16.67456354557714 31.277107555490762 31.983200675113054 20.06512822381904 70-71 18.390695027189036 30.761671519431854 31.35802192225576 19.48961153112335 72-73 17.52746112827529 30.08023864126991 32.01933913862613 20.372961091828667 74-75 16.80063024091015 31.867537266358088 31.2351936841338 20.096638808597966 76-77 17.08804333198762 30.496680572376977 32.13704974655722 20.278226349078185 78-79 16.754864926258403 30.204608744668143 32.231626297383784 20.808900031689674 80-81 17.92174143464222 31.343384026100207 30.515905340476213 20.218969198781355 82-83 17.64215254054342 30.230553246487453 31.890839408947098 20.23645480402203 84-85 16.905005894812987 30.126629835183554 31.28989162235627 21.678472647647194 86-87 17.145064021509658 30.940318551518605 31.465443524533406 20.449173902438343 88-89 17.72842157499057 29.435925328339145 31.04688636780994 21.78876672886034 90-91 17.06859570172856 28.212277971709355 32.718173024351266 22.00095330221082 92-93 18.04335879232417 30.029833002478608 31.757988178953156 20.16882002624407 94-95 17.074442657859283 28.48132940627274 32.30082106594182 22.143406869926164 96-97 16.31511882349808 28.881126037156086 32.404828356713004 22.39892678263283 98-99 16.663558805179147 29.70409887205659 31.37084732455187 22.261494998212395 100-101 16.14166658486547 29.097714100609863 32.1058012006546 22.65481811387007 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 741.0 1 528.0 2 327.5 3 353.5 4 311.5 5 318.5 6 535.0 7 980.5 8 1490.5 9 1671.0 10 1419.5 11 1121.5 12 1103.0 13 1137.5 14 1013.0 15 932.0 16 917.0 17 832.0 18 797.5 19 857.5 20 912.5 21 966.5 22 1015.5 23 1101.5 24 1196.5 25 1469.0 26 2214.0 27 2993.5 28 3226.0 29 3954.0 30 5971.0 31 7870.0 32 10906.0 33 15050.0 34 17972.0 35 19892.0 36 22885.0 37 25207.5 38 23742.5 39 21274.0 40 18427.5 41 15784.5 42 13105.0 43 12080.0 44 12065.0 45 10779.0 46 9168.0 47 9666.0 48 15573.0 49 14358.0 50 6584.0 51 3004.5 52 1527.5 53 1044.0 54 879.5 55 792.5 56 686.5 57 624.0 58 625.0 59 586.5 60 509.0 61 476.0 62 417.0 63 290.5 64 189.5 65 151.0 66 105.0 67 76.0 68 60.0 69 40.5 70 30.0 71 29.0 72 22.0 73 14.5 74 12.5 75 13.0 76 11.5 77 8.0 78 12.0 79 14.0 80 9.0 81 3.5 82 2.5 83 2.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01261571413352472 2 0.00756942848011483 3 0.007289079277147615 4 0.0333615551530987 5 0.04485587247475455 6 0.05915368182608257 7 0.02439038065814779 8 0.024670729861115005 9 0.01261571413352472 10-11 0.04443534867030373 12-13 0.042192555046566 14-15 0.019904793410672333 16-17 0.022427936237377277 18-19 0.027754571093754378 20-21 0.01948426960622151 22-23 0.013316587140942759 24-25 0.0032240158341229836 26-27 0.0025231428267049436 28-29 0.001121396811868864 30-31 0.006728380871213183 32-33 0.0012615714133524718 34-35 0.001962444420770512 36-37 0.004765936450442671 38-39 0.006588206269729575 40-41 0.002242793623737728 42-43 9.81222210385256E-4 44-45 0.0015419206163196878 46-47 0.0026633174281885516 48-49 7.008730074180399E-4 50-51 0.00210261902225412 52-53 7.008730074180399E-4 54-55 0.0025231428267049436 56-57 0.0016820952178032958 58-59 0.0026633174281885516 60-61 8.410476089016479E-4 62-63 4.2052380445082395E-4 64-65 0.0032240158341229836 66-67 0.002242793623737728 68-69 0.004765936450442671 70-71 0.007989952284565656 72-73 0.0033641904356065916 74-75 0.0025231428267049436 76-77 0.0028034920296721596 78-79 0.032240158341229834 80-81 0.020325317215123158 82-83 0.003504365037090199 84-85 0.007989952284565656 86-87 0.005887333262311535 88-89 0.004625761848959064 90-91 0.012055015727590285 92-93 0.012896063336491934 94-95 0.007709603081598439 96-97 0.003784714240057415 98-99 0.021166364824024807 100-101 0.03994976142282827 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 356698.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.47390229269578 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97685882514035 14.941210772137776 2 9.166096124880188 3.7533151293250877 3 3.8367794057236755 2.3566154001424175 4 2.1456935505956456 1.7572288041985098 5 1.4829522114199645 1.5180909340674744 6 1.0968095303300014 1.34735826946044 7 0.862659181158428 1.2363399850854224 8 0.6928659455018485 1.1348535736112901 9 0.5545666164589895 1.0218728448155021 >10 5.48541695193756 24.265905612030345 >50 1.0324524168150075 14.753937504555676 >100 0.6230316308366425 22.403545856719127 >500 0.03286320690127345 4.531003818356144 >1k 0.010954402300424484 4.9787214954947885 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3353 0.9400108775490751 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2632 0.7378791022097124 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2577 0.7224598960465156 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2351 0.6591009761759247 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2095 0.5873315802163174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.49621808925197225 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1728 0.48444342272734914 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1253 0.3512775513179216 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 951 0.2666120920218224 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 870 0.24390380658147787 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 856 0.23997891773993685 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 803 0.22512040998267444 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 792 0.22203656875003502 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 763 0.2139064418639858 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 701 0.19652479128001837 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 690 0.19344095004737902 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 682 0.1911981564236413 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 659 0.18475012475539532 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 651 0.1825073311316576 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 651 0.1825073311316576 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 649 0.18194663272572315 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 640 0.1794234898990182 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 639 0.179143140696051 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 628 0.17605929946341162 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 627 0.1757789502604444 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 624 0.17493790265154274 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 607 0.1701719662011001 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.1668077757654935 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.15755625206757537 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 512 0.1435387919192146 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 509 0.14269774431031293 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 501 0.1404549506865752 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 493 0.13821215706283746 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 489 0.1370907602509686 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 483 0.13540866503316532 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 450 0.1261571413352472 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 423 0.11858771285513235 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 415 0.11634491923139462 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 396 0.11101828437501751 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 394 0.11045758596908309 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 393 0.11017723676611586 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 390 0.10933618915721423 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 389 0.109055839954247 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 381 0.1068130463305093 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 379 0.10625234792457484 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 375 0.10513095111270598 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 369 0.10344885589490269 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.10316850669193547 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 368 0.10316850669193547 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 366 0.10260780828600105 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 359 0.10064536386523053 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 358 0.10036501466226332 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4017460148360797E-4 0.0 18-19 0.0 0.0 0.0 2.8034920296721595E-4 0.0 20-21 0.0 0.0 0.0 2.8034920296721595E-4 0.0 22-23 0.0 0.0 0.0 5.606984059344319E-4 0.0 24-25 0.0 0.0 0.0 0.0014017460148360798 0.0 26-27 0.0 0.0 0.0 0.0025231428267049436 0.0 28-29 0.0 0.0 0.0 0.005466809457860711 0.0 30-31 0.0 0.0 0.0 0.013316587140942759 0.0 32-33 0.0 0.0 0.0 0.022427936237377277 0.0 34-35 0.0 0.0 0.0 0.03195980913826262 0.0 36-37 0.0 0.0 0.0 0.04303360265546765 0.0 38-39 0.0 0.0 0.0 0.0562100151949268 0.0 40-41 0.0 0.0 0.0 0.07106852295218924 0.0 42-43 0.0 0.0 0.0 0.10499077651122238 0.0 44-45 0.0 0.0 0.0 0.14003442688212436 0.0 46-47 0.0 0.0 0.0 0.17325580743373947 0.0 48-49 0.0 0.0 0.0 0.21138329903728081 0.0 50-51 0.0 0.0 0.0 0.26170598096989606 0.0 52-53 0.0 0.0 0.0 0.3585666305950692 0.0 54-55 0.0 0.0 0.0 0.42585043930720107 0.0 56-57 0.0 0.0 0.0 0.5601377075284975 0.0 58-59 0.0 0.0 0.0 0.7122271501382123 0.0 60-61 0.0 0.0 0.0 0.8759510846710663 0.0 62-63 0.0 0.0 0.0 1.1020527168641259 0.0 64-65 0.0 0.0 0.0 1.227228635988988 0.0 66-67 0.0 0.0 0.0 1.5204739022926956 0.0 68-69 0.0 0.0 0.0 1.8559117236429696 0.0 70-71 0.0 0.0 0.0 2.06028629260607 0.0 72-73 0.0 0.0 0.0 2.441841557844451 0.0 74-75 0.0 0.0 0.0 2.8169487914145863 0.0 76-77 0.0 0.0 0.0 3.0266499952340635 0.0 78-79 0.0 0.0 0.0 3.2806463731223614 0.0 80-81 0.0 0.0 0.0 3.7268221296446855 0.0 82-83 0.0 0.0 0.0 4.094219760133222 0.0 84-85 0.0 0.0 0.0 4.674682784876842 0.0 86-87 0.0 0.0 0.0 5.512646552545851 0.0 88-89 0.0 0.0 0.0 6.590589237954797 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCAAG 25 0.005001514 57.04867 1 GATCCTC 35 2.7716867E-4 54.339695 6 TAGGCAT 75 2.3592293E-9 50.70993 5 AGGGTCT 40 5.360539E-4 47.54056 5 GTATGGT 60 1.4806301E-6 47.540558 1 CCTTTAG 75 1.3501631E-7 44.37119 1 CTTTAGG 85 3.607056E-7 39.15105 2 TACGAAC 50 0.0016086734 38.02711 8 GGATGCC 120 3.752575E-9 35.650417 8 GGTACTC 95 8.6292494E-7 35.02497 8 GGCATTC 95 8.6292494E-7 35.02497 7 GTATTAT 245 0.0 34.92776 1 TAGGGTC 55 0.0025664817 34.57495 4 GGGATTC 55 0.0025682491 34.570103 7 TGGTACT 85 1.6211241E-5 33.553333 7 GGGATGC 130 8.196366E-9 32.908077 7 CATATAG 60 0.0039308663 31.693707 1 GTCCTGA 60 0.0039308663 31.693707 1 ACCTAAC 60 0.0039335685 31.689262 8 ATTGTCG 30 0.0039524394 31.6626 32-33 >>END_MODULE