FastQCFastQC Report
Thu 26 May 2016
SRR936590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences356698
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39771.1149487802006177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35701.0008466545929608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.8873052273912385No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.6268608178346949No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19410.5441578029593661No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14950.4191220584359879No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14350.4023011062579549No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11470.3215605358033967No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC10660.29885225036305224No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10330.2896007266651341No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT10170.28511513941765865No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10170.28511513941765865No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9610.26941558405149457No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8730.24474485419037953No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8540.23941821933400245No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8080.2265221559975105No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7930.22231691795300224No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7920.22203656875003502No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7850.22007412432926451No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7830.21951342592333012No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA7390.2071780609927726No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7180.20129072773046106No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA7040.19736583888892004No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT6710.1881143151910019No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6370.17858244229011658No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG6000.16820952178032958No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC5960.1670881249684607No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG5720.16035974409724754No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA5200.1457815855429523No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5190.1455012363399851No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC5100.14297809351328014No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG5030.14101564909250963No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA4980.13961390307767355No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4950.1387728554687719No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT4870.1365300618450342No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT4580.1283999349589849No Hit
CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC4500.1261571413352472No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.1244750461174439No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4320.12111085568183728No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4310.12083050647887009No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4220.11830736365216514No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG4180.11718596684029627No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA4020.11270037959282082No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA3930.11017723676611586No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG3880.1087754907512798No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA3860.10821479234534535No Hit
ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT3850.10793444314237816No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG3810.1068130463305093No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG3770.10569164951864042No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.1054113003156732No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3750.10513095111270598No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC3680.10316850669193547No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT3680.10316850669193547No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA3600.10092571306819775No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT250.00479369457.6634981
GGGTCCT1100.047.4936947
GGGGTCC1305.456968E-1240.1869666
AGGGGTC1305.456968E-1240.1869665
GGGATGC608.285729E-539.5780757
TATAGGG1401.0913936E-1137.3217052
ATCTAGA550.00243500334.9475751
CCTTTAG550.00243500334.9475751
CATATAG1151.0341864E-733.039093
ACCCTTG1456.6211214E-1032.754277
AGGAGCC600.00394989831.6624627
GGTCCTA1657.6397555E-1131.6624628
GACATAT1251.9396612E-730.7538661
AGGCTTA3000.030.0793386
CACATTG404.4843805E-429.69604986-87
CTTTAGG650.005838528329.2309842
CTATACG650.00584253129.2268853
AGTACAC700.00838364227.1430592
ATACGTC700.0083893827.1392525
GCAGATC1109.467211E-525.9056518
Produced by FastQC (version 0.11.5)