##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 356698 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45878866716382 31.0 31.0 34.0 30.0 34.0 2 31.715728711683273 31.0 31.0 34.0 30.0 34.0 3 31.957869121778085 31.0 31.0 34.0 30.0 34.0 4 35.77706351030844 37.0 35.0 37.0 35.0 37.0 5 35.59873338230099 37.0 35.0 37.0 33.0 37.0 6 35.72599509949593 37.0 35.0 37.0 33.0 37.0 7 35.65988875743626 37.0 35.0 37.0 33.0 37.0 8 35.64820940964065 37.0 35.0 37.0 33.0 37.0 9 37.26940437008338 39.0 37.0 39.0 34.0 39.0 10-11 37.17312264156233 39.0 37.0 39.0 33.0 39.0 12-13 37.0867820957785 39.0 37.0 39.0 33.0 39.0 14-15 38.106738753791724 40.0 37.0 41.0 33.0 41.0 16-17 38.034889458309266 40.0 37.0 41.0 33.0 41.0 18-19 38.19498146891769 40.0 37.5 41.0 33.5 41.0 20-21 38.299326040516064 40.0 38.0 41.0 34.0 41.0 22-23 38.18989313088383 40.0 37.5 41.0 33.0 41.0 24-25 38.089809867170544 40.0 37.0 41.0 33.0 41.0 26-27 37.840645307795384 40.0 37.0 41.0 33.0 41.0 28-29 37.773875939870706 39.5 37.0 41.0 33.0 41.0 30-31 37.73045545531514 39.0 37.0 41.0 33.0 41.0 32-33 37.474962012682994 39.0 36.0 41.0 33.0 41.0 34-35 37.29999467336515 39.0 35.5 41.0 32.5 41.0 36-37 37.05527505060303 39.0 35.0 41.0 31.5 41.0 38-39 36.87390874072745 39.0 35.0 41.0 31.0 41.0 40-41 36.706761742426366 39.0 35.0 41.0 31.0 41.0 42-43 36.448450790304406 39.0 35.0 40.0 30.5 41.0 44-45 36.13009604763694 39.0 35.0 40.0 30.0 41.0 46-47 35.68698871314109 39.0 35.0 40.0 29.0 41.0 48-49 35.18031640211047 38.0 35.0 40.0 26.5 41.0 50-51 35.02060706816411 38.0 35.0 40.0 26.0 41.0 52-53 34.83638119641826 38.0 34.5 40.0 25.5 41.0 54-55 34.61474132179042 38.0 34.0 40.0 24.0 41.0 56-57 34.362705986576884 38.0 34.0 40.0 23.5 41.0 58-59 34.05596470964233 38.0 33.5 40.0 22.5 41.0 60-61 33.68292084620603 38.0 33.0 40.0 22.0 41.0 62-63 33.44253121688375 37.0 33.0 40.0 22.0 41.0 64-65 33.02072341308333 37.0 32.5 39.0 19.5 40.5 66-67 32.612480305468495 36.0 32.0 39.0 18.0 40.0 68-69 32.10946514979058 36.0 31.0 39.0 15.0 40.0 70-71 31.583580507880615 35.0 31.0 38.0 10.5 40.0 72-73 31.130020353352137 35.0 30.5 37.5 7.5 39.5 74-75 30.71838502038139 35.0 30.0 37.0 6.5 39.0 76-77 29.20412225468043 33.0 28.5 35.5 2.0 38.0 78-79 29.962236962360315 34.0 30.0 36.0 2.0 38.5 80-81 29.72482604331956 34.0 29.0 36.0 2.0 37.5 82-83 29.341828381431913 34.0 29.0 35.0 2.0 37.0 84-85 28.841325154612584 34.0 29.0 35.0 2.0 36.5 86-87 28.494381801972537 34.0 29.0 35.0 2.0 36.0 88-89 28.046826727371617 34.0 28.5 35.0 2.0 36.0 90-91 27.58451827596454 33.0 26.5 35.0 2.0 35.0 92-93 27.148838793601307 33.0 26.0 35.0 2.0 35.0 94-95 26.627230878782612 33.0 25.5 35.0 2.0 35.0 96-97 25.97075957813052 32.0 24.5 34.0 2.0 35.0 98-99 25.253521185989268 32.0 21.0 34.0 2.0 35.0 100-101 22.58234416789553 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.28455279425404356 1101 2 0.24831732925397532 1101 3 0.04962768121209038 1101 4 0.12436841518197639 1101 5 0.2867775886114785 1101 6 0.1541865710966519 1101 7 0.20631100177836004 1101 8 0.08564754529108143 1101 9 0.30290541178577257 1101 10-11 0.2847196837679391 1101 12-13 0.2708297540967308 1101 14-15 0.34604619416763427 1101 16-17 0.408521847372441 1101 18-19 0.21246238044029297 1101 20-21 0.044501978810664866 1101 22-23 0.014805411374574362 1101 24-25 -0.05737578008280053 1101 26-27 -0.05654207284273127 1101 28-29 0.10305054028215466 1101 30-31 0.010160232319670115 1101 32-33 0.054854552570724024 1101 34-35 0.24106295370025066 1101 36-37 0.40333676774437777 1101 38-39 0.33905941872553313 1101 40-41 0.4651181845533756 1101 42-43 0.6205715392529854 1101 44-45 0.45920377811150104 1101 46-47 0.3156663264909483 1101 48-49 0.4109613873147424 1101 50-51 0.5566031174607318 1101 52-53 0.6632222743555829 1101 54-55 0.9043400757414517 1101 56-57 0.6807946862830221 1101 58-59 0.8168481163612071 1101 60-61 1.1166704199240414 1101 62-63 0.8198329105115292 1101 64-65 0.9230355572745452 1101 66-67 0.6828164667561296 1101 68-69 0.7025724838022605 1101 70-71 0.6093586819291055 1101 72-73 0.8222213870748227 1101 74-75 0.6897643276974215 1101 76-77 1.1126375239907453 1101 78-79 1.0954453577631007 1101 80-81 0.996211239078427 1101 82-83 1.0842438156163787 1101 84-85 1.2396044088582912 1101 86-87 1.1282690188017312 1101 88-89 1.3339321243084896 1101 90-91 1.1660811608580417 1101 92-93 1.1577869653038597 1101 94-95 0.6697113101710563 1101 96-97 0.7640719448260533 1101 98-99 0.8882449313033796 1101 100-101 0.941454087859821 1102 1 0.5454637305646273 1102 2 0.4031416124308329 1102 3 0.33339449166628654 1102 4 0.24341180518451466 1102 5 0.27701166625674745 1102 6 0.3444111333904871 1102 7 0.22681658411786998 1102 8 0.05731082872168258 1102 9 0.14152187968731766 1102 10-11 0.2967153701419605 1102 12-13 0.30816640723045907 1102 14-15 0.3954086656215807 1102 16-17 0.6605153134977897 1102 18-19 0.3722489193897971 1102 20-21 0.4772078275800169 1102 22-23 0.09959987978360374 1102 24-25 0.1433695891128295 1102 26-27 0.17054496090504756 1102 28-29 0.07267753850594971 1102 30-31 0.48605467535443125 1102 32-33 0.6518239131391041 1102 34-35 0.5166085005157868 1102 36-37 0.4534969428268454 1102 38-39 0.6414826615573048 1102 40-41 0.5885846270806567 1102 42-43 0.4122027876670913 1102 44-45 0.6741254350513657 1102 46-47 0.8719765903833618 1102 48-49 0.8002613746276026 1102 50-51 0.7564365988106445 1102 52-53 0.5615348853680118 1102 54-55 0.583005071935311 1102 56-57 0.8737358814111644 1102 58-59 0.7634114886017542 1102 60-61 1.120019823628688 1102 62-63 0.8087538696589576 1102 64-65 0.7673533065767586 1102 66-67 0.9353776821836917 1102 68-69 0.8817386218382843 1102 70-71 0.6450872406705379 1102 72-73 0.9120367892570123 1102 74-75 0.2722751117623794 1102 76-77 0.6302298228996506 1102 78-79 0.7341125742056249 1102 80-81 1.0704325661527747 1102 82-83 1.17828561972701 1102 84-85 1.3658525057880055 1102 86-87 1.1367075496314705 1102 88-89 1.083121733544722 1102 90-91 1.1753047081810593 1102 92-93 1.4638482441670924 1102 94-95 1.2935345783770948 1102 96-97 1.1526075322404132 1102 98-99 1.069940566928853 1102 100-101 0.9573336235106744 1103 1 0.4001908133538663 1103 2 0.6092104293543166 1103 3 0.30834225870728815 1103 4 0.175939880399973 1103 5 0.0884213032376806 1103 6 0.09654615558949331 1103 7 0.027596813942892595 1103 8 0.08814786188960966 1103 9 -0.0377383710308834 1103 10-11 0.05253281595026493 1103 12-13 0.1690847279408274 1103 14-15 0.3672453070423174 1103 16-17 0.1844886093975049 1103 18-19 0.10044982040834327 1103 20-21 -0.06912117477046564 1103 22-23 -0.09107000791552622 1103 24-25 -0.09931453206763052 1103 26-27 0.1309654362249688 1103 28-29 0.3055843026741414 1103 30-31 0.18049186522124216 1103 32-33 0.32075672849559567 1103 34-35 0.431122733997114 1103 36-37 0.6662466816620594 1103 38-39 0.7529608542969228 1103 40-41 0.7544291687364364 1103 42-43 0.786029178369624 1103 44-45 0.3956437818782135 1103 46-47 0.5816870239169205 1103 48-49 0.5070173814178887 1103 50-51 0.279642723125086 1103 52-53 0.2539480480736742 1103 54-55 0.4529923401515603 1103 56-57 0.4551719613275935 1103 58-59 0.61130747972215 1103 60-61 0.6687792908525054 1103 62-63 0.5804860451616989 1103 64-65 0.5973725674803845 1103 66-67 0.6861366923691179 1103 68-69 0.845673395930735 1103 70-71 0.8215024274033702 1103 72-73 0.7175571601110207 1103 74-75 0.723156542945457 1103 76-77 0.7056460542916625 1103 78-79 1.081099385383478 1103 80-81 1.075445297936529 1103 82-83 0.8155750556110561 1103 84-85 1.012720160528147 1103 86-87 1.1009132821987215 1103 88-89 1.2204274922284526 1103 90-91 0.9693370926156355 1103 92-93 0.9139167999257758 1103 94-95 1.1017384174995826 1103 96-97 0.9504088511226279 1103 98-99 0.9433899009586248 1103 100-101 0.6037213879400305 1104 1 0.3726759919874141 1104 2 0.28098285014049296 1104 3 0.18306192949161826 1104 4 0.04396125573396148 1104 5 -0.004285326224454877 1104 6 -0.009594071930507653 1104 7 0.014229828767959418 1104 8 -0.11066198793534454 1104 9 0.13363437245770626 1104 10-11 -0.01587861482044417 1104 12-13 -0.1342388848458853 1104 14-15 0.20796650252152205 1104 16-17 0.025274567401780246 1104 18-19 0.12450817329379049 1104 20-21 0.01262208437585599 1104 22-23 0.10620774560199919 1104 24-25 0.10780712526089786 1104 26-27 0.15070690422488298 1104 28-29 0.3455520324330834 1104 30-31 -0.03433283187518299 1104 32-33 -0.2599928884803333 1104 34-35 -0.23661590665599164 1104 36-37 0.14030651483378875 1104 38-39 0.4447504464040364 1104 40-41 0.3836359573293535 1104 42-43 0.22322433191231994 1104 44-45 0.4838817971103566 1104 46-47 0.639099927572488 1104 48-49 0.7878517447837723 1104 50-51 0.7526663558956628 1104 52-53 0.903640148525902 1104 54-55 1.0727715554518014 1104 56-57 0.8844031746442482 1104 58-59 0.8932372549406793 1104 60-61 0.7606791642880211 1104 62-63 0.4196344364605764 1104 64-65 0.5754902354373641 1104 66-67 0.8042505855960727 1104 68-69 0.9447989168064801 1104 70-71 0.8577065292766726 1104 72-73 1.0526310634039149 1104 74-75 0.6507742442088791 1104 76-77 1.0237484752998824 1104 78-79 0.8930845233091524 1104 80-81 0.9941276210978245 1104 82-83 0.8934714207680763 1104 84-85 0.8045345474305741 1104 86-87 0.8320914420548355 1104 88-89 0.5162442031803494 1104 90-91 0.4936100870127511 1104 92-93 0.531408972912029 1104 94-95 0.6461155500370133 1104 96-97 0.7617204587875541 1104 98-99 1.0365036091497934 1104 100-101 0.5417140457200453 1105 1 0.4783333773292391 1105 2 0.4659463951754468 1105 3 0.22643137819332892 1105 4 0.1378006767068598 1105 5 0.01419906856997244 1105 6 -0.029088397712982328 1105 7 0.09413840343641056 1105 8 -0.06903334133146188 1105 9 0.21411878497021775 1105 10-11 0.17316894938547733 1105 12-13 0.04457426906228079 1105 14-15 0.07446071313642477 1105 16-17 0.29133158420739136 1105 18-19 0.255444839989984 1105 20-21 0.3132249423033713 1105 22-23 0.321442643147158 1105 24-25 0.17037412177929667 1105 26-27 0.08268938103633161 1105 28-29 0.08339963916240833 1105 30-31 0.2206592392806641 1105 32-33 0.3503332129202832 1105 34-35 0.3266819609721807 1105 36-37 0.4055307815264442 1105 38-39 0.23974774439569302 1105 40-41 0.45415394561769773 1105 42-43 0.5886465982328914 1105 44-45 0.5771830311751529 1105 46-47 0.7861822015118349 1105 48-49 0.8864152508389225 1105 50-51 0.7780585900579027 1105 52-53 0.9504845571404346 1105 54-55 1.0436939590875483 1105 56-57 1.1807869129835353 1105 58-59 1.2147341675639254 1105 60-61 1.535212071206054 1105 62-63 1.2365711563110366 1105 64-65 1.3889010558734114 1105 66-67 1.3192378728682783 1105 68-69 1.1178182560989889 1105 70-71 1.299254558582394 1105 72-73 0.9549290993539152 1105 74-75 0.9916170936317137 1105 76-77 0.974620648157547 1105 78-79 0.8717297983381656 1105 80-81 1.0361659984753757 1105 82-83 1.060043590968025 1105 84-85 1.2360209177917554 1105 86-87 1.0249949050550384 1105 88-89 0.8148914271989902 1105 90-91 0.5169270901754288 1105 92-93 0.7123438677994791 1105 94-95 0.7485947534317994 1105 96-97 0.5404924181422608 1105 98-99 1.0023608545187237 1105 100-101 1.2292799348579706 1106 1 0.3816530756613332 1106 2 0.2789008591223876 1106 3 0.0038135619072114935 1106 4 -0.10102321347853405 1106 5 -0.07710692892479187 1106 6 -0.11290987102078276 1106 7 -0.04657584886482624 1106 8 -1.2894053519119097E-4 1106 9 -0.0036808688705392 1106 10-11 -0.08788761594558281 1106 12-13 -0.18996940885369185 1106 14-15 -0.1655898074018367 1106 16-17 -0.09423315952562206 1106 18-19 -0.10248173890676782 1106 20-21 0.049465632127017045 1106 22-23 -0.005498525374179053 1106 24-25 0.05329069532253783 1106 26-27 0.10534306066751498 1106 28-29 -0.04679989617338265 1106 30-31 -0.11048417771647934 1106 32-33 0.17164279674955907 1106 34-35 -0.040899134367151646 1106 36-37 -0.06322132999297025 1106 38-39 0.0894127599624639 1106 40-41 0.5130607892794998 1106 42-43 0.2100544164421123 1106 44-45 0.15680485480026363 1106 46-47 0.244242878133619 1106 48-49 0.11732321717935434 1106 50-51 0.13281724014080964 1106 52-53 0.45286505163879553 1106 54-55 0.327887579570195 1106 56-57 0.3436480802574522 1106 58-59 0.6368951160194669 1106 60-61 0.4546312929194869 1106 62-63 0.053795437354921205 1106 64-65 0.40335814200260955 1106 66-67 0.1865123106193849 1106 68-69 0.514362900766006 1106 70-71 0.3287846448103444 1106 72-73 0.36309294806257597 1106 74-75 0.601686377456307 1106 76-77 0.301821695990931 1106 78-79 0.26671776592602114 1106 80-81 0.22786597300720146 1106 82-83 0.38210942359535593 1106 84-85 0.439722118953938 1106 86-87 0.5819760430145706 1106 88-89 0.36564845563498594 1106 90-91 0.46493823380033206 1106 92-93 0.5809800731457138 1106 94-95 0.9669609441728184 1106 96-97 0.6531059204317948 1106 98-99 0.7009424332335712 1106 100-101 1.084558343564968 1107 1 0.7466456421781942 1107 2 0.5672089300053464 1107 3 0.34601011345457167 1107 4 0.14634089048769994 1107 5 -0.11806798430851728 1107 6 -0.08341517698978151 1107 7 0.07644402502229042 1107 8 -0.10324929460822574 1107 9 0.00576905337693745 1107 10-11 0.2619517524646753 1107 12-13 0.36546985019037237 1107 14-15 0.2744040407500492 1107 16-17 0.06518514905791761 1107 18-19 0.33348350315136344 1107 20-21 0.23497375769306217 1107 22-23 0.29561735152049806 1107 24-25 0.3316660947654597 1107 26-27 0.03349460119281389 1107 28-29 0.28346890541242686 1107 30-31 0.3378018490440482 1107 32-33 0.3292070066642836 1107 34-35 0.11344817163602983 1107 36-37 0.4591688338854283 1107 38-39 0.16969437789851582 1107 40-41 0.5493438768237198 1107 42-43 0.15216296238445892 1107 44-45 -0.33816259167011253 1107 46-47 0.048421862736901744 1107 48-49 0.24642658531619333 1107 50-51 0.1200606995300646 1107 52-53 -0.14889676930824436 1107 54-55 -0.04897719939048528 1107 56-57 -0.015350706461390473 1107 58-59 -0.08361446206628642 1107 60-61 -0.02473865530245689 1107 62-63 -0.11687562674391927 1107 64-65 0.41118038776894394 1107 66-67 0.18014258454751086 1107 68-69 0.20846652522985565 1107 70-71 0.28295337633344175 1107 72-73 0.2095573442415919 1107 74-75 0.31592876027214345 1107 76-77 -0.10351246898112976 1107 78-79 -0.13802257481801306 1107 80-81 0.09000476553612202 1107 82-83 -0.07214475026270861 1107 84-85 -0.1708317182613257 1107 86-87 0.029095781902796602 1107 88-89 0.24179979109867844 1107 90-91 0.114616116197503 1107 92-93 0.022751121864935442 1107 94-95 0.24128637693824828 1107 96-97 0.18886780973270945 1107 98-99 0.8386327560347091 1107 100-101 0.692501917446954 1108 1 0.20908499302309735 1108 2 0.13515988485608332 1108 3 -0.24243607311776572 1108 4 -0.2687144406626629 1108 5 -0.47386042676650675 1108 6 -0.305671923087921 1108 7 -0.35229471156318937 1108 8 0.18912145995091834 1108 9 -0.1059448977213222 1108 10-11 -0.09954708832980685 1108 12-13 -0.18317495489578306 1108 14-15 -0.19940030447808965 1108 16-17 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-1.4678601611489341 2316 48-49 -0.9926930100965805 2316 50-51 -0.8842605959088345 2316 52-53 -0.9293892070412326 2316 54-55 -0.6807826461222035 2316 56-57 -1.0098125627189631 2316 58-59 -1.091152985702827 2316 60-61 -1.3600367534575994 2316 62-63 -1.1648847442024248 2316 64-65 -1.1235383718768048 2316 66-67 -1.029600512064306 2316 68-69 -1.3589349990952364 2316 70-71 -1.4939656824789154 2316 72-73 -1.7519012116859436 2316 74-75 -1.94162163505516 2316 76-77 -1.7471658630975213 2316 78-79 -1.6537881801555265 2316 80-81 -1.6884694847666069 2316 82-83 -1.7540546678731737 2316 84-85 -1.8102053678565042 2316 86-87 -2.0145801684260505 2316 88-89 -1.9372371773463648 2316 90-91 -2.060598167820828 2316 92-93 -2.6370180924665725 2316 94-95 -3.0413251575965035 2316 96-97 -2.6322080028845285 2316 98-99 -2.7180426956831205 2316 100-101 -2.808204448153635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 3.0 5 13.0 6 34.0 7 71.0 8 125.0 9 273.0 10 450.0 11 754.0 12 1082.0 13 1516.0 14 1953.0 15 2754.0 16 3797.0 17 5190.0 18 4106.0 19 2552.0 20 2334.0 21 2413.0 22 2662.0 23 2998.0 24 3442.0 25 3883.0 26 4597.0 27 5496.0 28 6568.0 29 8137.0 30 9959.0 31 12112.0 32 15228.0 33 19123.0 34 24816.0 35 34347.0 36 47820.0 37 70719.0 38 52131.0 39 3237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.87059677455939 24.119638442171162 17.134869689477625 18.87489509379182 2 14.211292208393031 25.92716169498084 39.74787818972503 20.113667906901107 3 17.968027184182933 30.868458385434483 32.66335462237648 18.5001598080061 4 11.119615990668933 21.181636189290582 43.21843334510932 24.480314474931166 5 9.945948673667921 41.5760671492411 37.19140561483384 11.286578562257148 6 24.674935099159512 43.6736398858418 18.29502828723458 13.356396727764103 7 23.522419525761286 36.36577721209539 25.376649154186453 14.735154107956872 8 23.37327374978273 35.08233856091147 22.659785028231163 18.884602661074634 9 24.89416342848812 19.600933043999976 25.299286198911076 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29.683805048858336 34.53725807378583 16.831069724932682 36-37 19.163159857241784 29.172776131454185 34.56658078371233 17.0974832275917 38-39 17.91910512408974 31.024888035380123 34.61672705864213 16.439279781888015 40-41 17.693722437009892 30.65761474474533 34.90942938127132 16.739233436973446 42-43 17.684878444061557 31.028483386210155 33.013922749837725 18.272715419890563 44-45 18.44161815355329 30.46801078245706 33.12101107262458 17.969359991365064 46-47 19.261354636403524 30.87510705970252 32.90584213056868 16.957696173325278 48-49 19.001242103669394 31.05259173482348 32.3598848180164 17.586281343490732 50-51 18.070824916981245 31.148189006620473 32.77716490069288 18.003821175705397 52-53 18.810478020799604 31.776236754936715 31.961271638217553 17.452013586046128 54-55 18.841660626639396 30.45729899326648 32.28627065584181 18.41476972425231 56-57 19.64660695011004 30.887197387050197 31.2065267673157 18.25966889552406 58-59 19.777373137592146 29.988323373858094 32.05535253458482 18.178950953964936 60-61 19.250993558516566 31.535512269659137 31.553315716799023 17.660178455025267 62-63 18.890617771983685 30.97130521328343 32.787894080210826 17.350182934522056 64-65 18.576091437031884 31.24079895237748 32.01123333996977 18.171876270620864 66-67 18.660338055494034 30.808103463260505 31.201445525068195 19.330112956177263 68-69 18.147054783191336 30.927089378379968 32.15810617975481 18.76774965867389 70-71 19.60801079918754 30.484856045876924 32.121975870039684 17.785157284895856 72-73 19.14168288019557 29.550488087962368 32.37542683166152 18.932402200180544 74-75 18.39188893685975 31.90233755165434 31.11749995794762 18.588273553538286 76-77 18.95061754353503 30.358807442202103 31.797429460141775 18.89314555412109 78-79 18.328218601175664 29.970881776085484 32.20823245230962 19.492667170429232 80-81 19.460922110605605 31.169777989639048 30.593125880645772 18.776174019109575 82-83 19.49156581913617 30.208835701708146 31.553874451834325 18.745724027321362 84-85 18.053661073642036 30.533316323884215 31.230049528338256 20.182973074135496 86-87 18.39247562062952 30.767915122219293 30.92212620635125 19.917483050799937 88-89 19.076366043207436 29.819048179964607 30.918585703908754 20.186000072919207 90-91 18.603663102279132 28.36226067984172 32.594515161967905 20.439561055911245 92-93 19.45826756642341 30.504445048767977 31.232109765089476 18.80517761971914 94-95 18.849927593018087 29.026297823304535 31.789118345111444 20.334656238565934 96-97 18.318302320332315 29.05665614752777 31.889954198986963 20.73508733315295 98-99 18.25113660419257 30.15271081677085 30.842734354956967 20.753418224079606 100-101 17.716587316274403 29.64984886974759 31.228865597819688 21.40469821615832 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1050.0 1 697.0 2 383.0 3 413.0 4 349.0 5 353.0 6 580.5 7 986.0 8 1340.5 9 1504.5 10 1409.5 11 1223.5 12 1129.0 13 1059.0 14 1026.0 15 979.0 16 945.5 17 970.5 18 924.5 19 902.0 20 994.0 21 1072.5 22 1157.5 23 1273.5 24 1368.5 25 1648.5 26 2341.5 27 3122.5 28 3384.0 29 4066.0 30 6031.0 31 7604.0 32 10510.0 33 14580.5 34 17554.0 35 19990.5 36 23006.5 37 24796.5 38 23187.0 39 20959.5 40 18610.5 41 15882.0 42 12765.5 43 11692.0 44 11980.5 45 10934.5 46 9231.0 47 10196.0 48 16295.0 49 14573.5 50 6499.5 51 2873.5 52 1415.0 53 1055.5 54 873.0 55 727.5 56 622.0 57 591.5 58 590.0 59 566.5 60 483.0 61 439.0 62 392.5 63 286.0 64 203.5 65 140.5 66 91.5 67 74.5 68 63.5 69 42.5 70 24.5 71 25.5 72 24.5 73 12.5 74 6.0 75 9.0 76 9.0 77 6.5 78 10.5 79 13.5 80 8.5 81 3.0 82 1.5 83 1.0 84 1.0 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.1219575102747983 2 0.01261571413352472 3 0.005606984059344319 4 0.011213968118688638 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004485587247475456 10-11 1.40174601483608E-4 12-13 1.40174601483608E-4 14-15 1.40174601483608E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.410476089016479E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004625761848959064 34-35 9.81222210385256E-4 36-37 0.0030838412326393756 38-39 1.40174601483608E-4 40-41 0.00420523804450824 42-43 0.014437983952811623 44-45 0.00210261902225412 46-47 0.0012615714133524718 48-49 0.01261571413352472 50-51 4.2052380445082395E-4 52-53 0.0023829682252213356 54-55 0.013877285546877189 56-57 0.0036445396385738076 58-59 7.008730074180399E-4 60-61 0.007148904675664007 62-63 0.0036445396385738076 64-65 0.02270828544034449 66-67 0.0030838412326393756 68-69 2.80349202967216E-4 70-71 0.0012615714133524718 72-73 0.0 74-75 0.0 76-77 7.008730074180399E-4 78-79 0.013596936343909976 80-81 0.019764618809188724 82-83 0.01513885696022966 84-85 0.009391698299401735 86-87 0.012335364930557502 88-89 0.03896853921244302 90-91 0.032240158341229834 92-93 0.0025231428267049436 94-95 0.009391698299401735 96-97 0.013036237937975542 98-99 0.012195190329073895 100-101 0.01457815855429523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 356698.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.5288451294933 #Duplication Level Percentage of deduplicated Percentage of total 1 71.20687922594352 13.905881165579848 2 9.995836862429837 3.90414300052145 3 4.104279418309192 2.4045551138498116 4 2.285418969551673 1.7852637244952314 5 1.5748144532651918 1.5377153782751796 6 1.1671140843250694 1.3675434120740795 7 0.9000990539628763 1.230452651823111 8 0.7034266928896481 1.0989688756314866 9 0.5756614364260182 1.0117802735086823 >10 5.778147834450681 24.244879421807806 >50 0.997717452159807 13.810562436570992 >100 0.6646664465467492 23.31496111556555 >500 0.028711293587332577 3.9075071909570562 >1k 0.017226776152399547 6.475786239339722 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3977 1.1149487802006177 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3570 1.0008466545929608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3165 0.8873052273912385 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2236 0.6268608178346949 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1941 0.5441578029593661 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1495 0.4191220584359879 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1435 0.4023011062579549 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1147 0.3215605358033967 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1066 0.29885225036305224 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1033 0.2896007266651341 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1017 0.28511513941765865 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1017 0.28511513941765865 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 961 0.26941558405149457 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 873 0.24474485419037953 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 854 0.23941821933400245 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 808 0.2265221559975105 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 793 0.22231691795300224 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 792 0.22203656875003502 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 785 0.22007412432926451 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 783 0.21951342592333012 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 739 0.2071780609927726 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 718 0.20129072773046106 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 704 0.19736583888892004 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 671 0.1881143151910019 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 637 0.17858244229011658 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 600 0.16820952178032958 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 596 0.1670881249684607 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 572 0.16035974409724754 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 520 0.1457815855429523 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 519 0.1455012363399851 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 510 0.14297809351328014 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 503 0.14101564909250963 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 498 0.13961390307767355 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 495 0.1387728554687719 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 487 0.1365300618450342 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 458 0.1283999349589849 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 450 0.1261571413352472 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.1244750461174439 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 432 0.12111085568183728 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 431 0.12083050647887009 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 422 0.11830736365216514 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 418 0.11718596684029627 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 402 0.11270037959282082 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 393 0.11017723676611586 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 388 0.1087754907512798 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 386 0.10821479234534535 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 385 0.10793444314237816 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 381 0.1068130463305093 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 377 0.10569164951864042 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 376 0.1054113003156732 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 375 0.10513095111270598 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 368 0.10316850669193547 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 368 0.10316850669193547 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 360 0.10092571306819775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.4017460148360797E-4 0.0 18-19 0.0 0.0 0.0 2.8034920296721595E-4 0.0 20-21 0.0 0.0 0.0 2.8034920296721595E-4 0.0 22-23 0.0 0.0 0.0 5.606984059344319E-4 0.0 24-25 0.0 0.0 0.0 0.0014017460148360798 0.0 26-27 0.0 0.0 0.0 0.0025231428267049436 0.0 28-29 0.0 0.0 0.0 0.005606984059344319 0.0 30-31 0.0 0.0 0.0 0.01387728554687719 0.0 32-33 0.0 0.0 0.0 0.022568110838860886 0.0 34-35 0.0 0.0 0.0 0.03209998373974623 0.0 36-37 0.0 0.0 0.0 0.043734475662885686 0.0 38-39 0.0 0.0 0.0 0.05775193581124649 0.0 40-41 0.0 0.0 0.0 0.07275061816999254 0.0 42-43 0.0 0.0 0.0 0.10695322093199289 0.0 44-45 0.0 0.0 0.0 0.14255756970882932 0.0 46-47 0.0 0.0 0.0 0.17661999786934607 0.0 48-49 0.0 0.0 0.0 0.21530818787882186 0.0 50-51 0.0 0.0 0.0 0.2664719174203388 0.0 52-53 0.0 0.0 0.0 0.3608094242188069 0.0 54-55 0.0 0.0 0.0 0.42963515354725845 0.0 56-57 0.0 0.0 0.0 0.5642027709715222 0.0 58-59 0.0 0.0 0.0 0.715871689776786 0.0 60-61 0.0 0.0 0.0 0.880717021121509 0.0 62-63 0.0 0.0 0.0 1.1093417961412735 0.0 64-65 0.0 0.0 0.0 1.2354989374765208 0.0 66-67 0.0 0.0 0.0 1.528604029178745 0.0 68-69 0.0 0.0 0.0 1.8648828981379206 0.0 70-71 0.0 0.0 0.0 2.0675753718832177 0.0 72-73 0.0 0.0 0.0 2.4505323831364345 0.0 74-75 0.0 0.0 0.0 2.8271815373228892 0.0 76-77 0.0 0.0 0.0 3.037022915743851 0.0 78-79 0.0 0.0 0.0 3.290458595226214 0.0 80-81 0.0 0.0 0.0 3.733690685117382 0.0 82-83 0.0 0.0 0.0 4.101368664808886 0.0 84-85 0.0 0.0 0.0 4.682392387958441 0.0 86-87 0.0 0.0 0.0 5.523580171461573 0.0 88-89 0.0 0.0 0.0 6.596897095021559 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTATT 25 0.004793694 57.663498 1 GGGTCCT 110 0.0 47.493694 7 GGGGTCC 130 5.456968E-12 40.186966 6 AGGGGTC 130 5.456968E-12 40.186966 5 GGGATGC 60 8.285729E-5 39.578075 7 TATAGGG 140 1.0913936E-11 37.321705 2 ATCTAGA 55 0.002435003 34.947575 1 CCTTTAG 55 0.002435003 34.947575 1 CATATAG 115 1.0341864E-7 33.03909 3 ACCCTTG 145 6.6211214E-10 32.75427 7 AGGAGCC 60 0.003949898 31.662462 7 GGTCCTA 165 7.6397555E-11 31.662462 8 GACATAT 125 1.9396612E-7 30.753866 1 AGGCTTA 300 0.0 30.079338 6 CACATTG 40 4.4843805E-4 29.696049 86-87 CTTTAGG 65 0.0058385283 29.230984 2 CTATACG 65 0.005842531 29.226885 3 AGTACAC 70 0.008383642 27.143059 2 ATACGTC 70 0.00838938 27.139252 5 GCAGATC 110 9.467211E-5 25.905651 8 >>END_MODULE