FastQCFastQC Report
Thu 26 May 2016
SRR936589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371459
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.9204245959850212No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26920.7247098603075979No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.718518059866634No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23430.6307560188338417No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20350.5478397346678906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17740.4775762600986919No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17610.47407654680597316No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12670.34108744168266214No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10230.2754005152654802No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9300.25036410478680016No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9030.2430954694865382No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8360.22505848559329564No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8080.21752064157820916No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT8040.21644380671891109No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT7270.19571473567742337No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7170.1930226485291782No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7010.18871530909198592No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7000.18844610037716142No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC6630.17848537792865432No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA6580.17713933435453172No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA6550.17633170821005817No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA6550.17633170821005817No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6540.17606249949523367No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG6500.1749856646359356No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6480.17444724720628654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.1685246554801472No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT6200.16690940319120012No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.15802551560199107No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5800.15614105459821948No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT5390.14510349729041427No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC4860.13083543540471493No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4850.1305662266898904No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4820.12975860054541685No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4770.12841255697129428No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT4630.12464363496375104No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC4620.12437442624892653No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA4540.1222207565303304No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG4380.11791341709313813No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4350.11710579094866458No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4270.11495212123006845No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC4210.11333686894112137No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC4210.11333686894112137No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4190.11279845151147233No Hit
CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA4170.11226003408182329No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT3990.10741427721498199No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT3980.10714506850015748No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA3970.10687585978533297No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.10526060749638587No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG3900.10499139878156136No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG3840.10337614649261426No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG3830.10310693777778975No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC3740.10068405934436911No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAA156.598803E-495.056311
GTATGGT558.142233E-751.84891
ATGTATT502.8133949E-547.528161
GGTACTC601.4876005E-647.5089578
TGGGATA1101.6079866E-938.8761986
AGGGTCT550.00256990534.5659335
AATGGTG851.6226433E-533.5492865
GGGTAGG1308.20728E-932.904111
CATGGGG4350.032.778044
CCCGCGC300.003949018731.66837188-89
CACTGAA300.00395422831.65984342-43
CATGGGT1951.6370905E-1129.2480984
CCCAATG650.00582215629.2480982
GGGATAG650.005829828329.2402177
GGATATG1004.924264E-528.5168951
GTACATG17400.027.3150331
GTGGTAC1056.878305E-527.1516326
TACAATC1056.878305E-527.1516327
ACAATCA1056.8846166E-527.1479748
CCGATGA350.00839327.14066522-23
Produced by FastQC (version 0.11.5)