##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936589_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 371459 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.670580064017834 31.0 31.0 34.0 30.0 34.0 2 31.740410112556162 33.0 31.0 34.0 30.0 34.0 3 31.760094653784133 33.0 31.0 34.0 30.0 34.0 4 35.41682931359854 37.0 35.0 37.0 33.0 37.0 5 35.40972489561432 37.0 35.0 37.0 33.0 37.0 6 35.54470614522733 37.0 35.0 37.0 33.0 37.0 7 35.447914843899326 37.0 35.0 37.0 33.0 37.0 8 35.40786197130774 37.0 35.0 37.0 33.0 37.0 9 36.92395123014922 39.0 37.0 39.0 33.0 39.0 10-11 36.85958342643468 39.0 37.0 39.0 33.0 39.0 12-13 36.81769993458228 39.0 37.0 39.0 33.0 39.0 14-15 37.874624117331926 40.0 37.5 41.0 33.0 41.0 16-17 37.78138502499603 40.0 37.0 41.0 32.5 41.0 18-19 37.85862908154063 40.0 37.5 41.0 33.0 41.0 20-21 37.80835570009072 40.0 37.0 41.0 32.5 41.0 22-23 37.68831149601975 40.0 37.0 41.0 32.0 41.0 24-25 37.79971544638843 40.0 37.0 41.0 33.0 41.0 26-27 37.76580322458199 40.0 37.0 41.0 33.0 41.0 28-29 37.70302779041563 40.0 37.0 41.0 33.0 41.0 30-31 37.5371656091251 39.5 37.0 41.0 33.0 41.0 32-33 37.33514600534649 39.0 36.0 41.0 32.0 41.0 34-35 37.12383735486285 39.0 36.0 41.0 31.5 41.0 36-37 36.9267456166091 39.0 35.0 41.0 31.0 41.0 38-39 36.74324218823612 39.0 35.0 41.0 31.0 41.0 40-41 36.65921811020866 39.0 35.0 41.0 31.0 41.0 42-43 36.436954011075244 39.0 35.0 40.5 30.0 41.0 44-45 36.17127731458923 39.0 35.0 40.0 30.0 41.0 46-47 35.825848882380015 39.0 35.0 40.0 29.5 41.0 48-49 35.66077817471107 39.0 35.0 40.5 28.0 41.0 50-51 34.75186494337195 38.0 34.0 40.0 26.0 40.5 52-53 34.938761747595294 38.0 34.5 39.5 27.0 40.5 54-55 35.41865993285935 39.0 35.0 40.0 27.0 41.0 56-57 35.34901429229067 39.0 35.0 41.0 27.0 41.0 58-59 35.14745234332726 39.0 35.0 40.5 26.0 41.0 60-61 34.85586700012653 38.5 35.0 40.0 26.0 41.0 62-63 34.5659251761298 38.0 34.0 40.0 25.0 41.0 64-65 34.25241951332448 38.0 34.0 40.0 23.5 41.0 66-67 33.84931984418199 37.5 34.0 40.0 22.0 41.0 68-69 33.42617220204653 37.0 33.0 39.0 21.5 41.0 70-71 32.933304886945805 36.0 33.0 39.0 19.5 41.0 72-73 32.48641976638068 36.0 32.5 39.0 18.0 40.0 74-75 32.023582683418624 35.0 32.0 38.0 14.5 40.0 76-77 31.553960194799423 35.0 31.5 37.0 9.5 39.0 78-79 31.06579999407741 35.0 31.0 37.0 7.0 39.0 80-81 30.533057484136876 35.0 31.0 36.0 2.0 38.5 82-83 29.99474369984305 34.5 30.0 36.0 2.0 37.0 84-85 29.601514568229604 34.0 30.0 35.0 2.0 37.0 86-87 29.32079045062847 34.0 30.0 35.0 2.0 36.5 88-89 28.879060676952236 34.0 29.0 35.0 2.0 36.0 90-91 28.450985169291897 34.0 29.0 35.0 2.0 36.0 92-93 28.101359773218576 34.0 29.0 35.0 2.0 35.5 94-95 27.68260696335262 34.0 28.0 35.0 2.0 35.0 96-97 27.15081206808827 34.0 26.5 35.0 2.0 35.0 98-99 26.57589262879618 34.0 25.5 35.0 2.0 35.0 100-101 24.1961373933597 31.5 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1103282534657204 1101 2 0.29682645827146814 1101 3 0.2917200918014551 1101 4 0.2782857537006791 1101 5 0.28179089274185287 1101 6 0.12486526991406777 1101 7 0.16244799593307135 1101 8 0.28277044408267216 1101 9 0.5203854820438849 1101 10-11 0.35185050529961615 1101 12-13 0.4919518052635894 1101 14-15 0.5720858067883512 1101 16-17 0.5100620895911376 1101 18-19 0.6898111821719937 1101 20-21 0.5230242332599531 1101 22-23 0.5440937076141168 1101 24-25 0.7107039238310762 1101 26-27 0.4184040419616366 1101 28-29 0.5123879129483768 1101 30-31 0.6035264077524403 1101 32-33 0.3861706592650407 1101 34-35 0.563935480893619 1101 36-37 0.6013904978954514 1101 38-39 0.8033744357457238 1101 40-41 0.6343471182392619 1101 42-43 0.7944600114575309 1101 44-45 0.8461798304840116 1101 46-47 1.1459749791920188 1101 48-49 1.0330003188836585 1101 50-51 1.0014743198111162 1101 52-53 0.963944499834831 1101 54-55 0.9383743829043283 1101 56-57 1.2977788071492782 1101 58-59 1.2117074674533228 1101 60-61 1.3020600878051667 1101 62-63 1.3498995638808466 1101 64-65 1.193191354424222 1101 66-67 1.1837056870077092 1101 68-69 1.3183131515155324 1101 70-71 1.5243440748672867 1101 72-73 1.4842078967430083 1101 74-75 1.2260182457861255 1101 76-77 1.2956607064153012 1101 78-79 1.2847566017565448 1101 80-81 1.258306135472516 1101 82-83 1.3411148440266558 1101 84-85 1.2812698006012084 1101 86-87 1.2864466273086173 1101 88-89 1.0858019053613255 1101 90-91 1.1955632770907023 1101 92-93 1.342627684584997 1101 94-95 1.1555206786451038 1101 96-97 1.0200061526922113 1101 98-99 1.260697225601671 1101 100-101 1.8591101889823598 1102 1 0.38650288565505875 1102 2 0.28151156852909054 1102 3 0.3994412060846244 1102 4 0.3360982643978261 1102 5 0.4269639982345339 1102 6 0.3684829248258552 1102 7 0.3887208707097187 1102 8 0.37958218116223463 1102 9 0.2930856812675273 1102 10-11 0.4142585098454745 1102 12-13 0.44288185791065615 1102 14-15 0.49644414919286817 1102 16-17 0.5884524857791931 1102 18-19 0.7944104414160336 1102 20-21 0.9787979535390008 1102 22-23 0.49520860694113367 1102 24-25 0.38850931450795656 1102 26-27 0.33677711733115956 1102 28-29 0.5395826249640976 1102 30-31 0.48144886799327935 1102 32-33 0.47933338595895947 1102 34-35 0.048336474957984876 1102 36-37 0.396296260319545 1102 38-39 0.4237986246453289 1102 40-41 0.5664074056416126 1102 42-43 0.7026870579499018 1102 44-45 0.5704450649312207 1102 46-47 0.5026606097588129 1102 48-49 0.6583143870852339 1102 50-51 0.7377853962349548 1102 52-53 0.7054620910772016 1102 54-55 0.6589666453592287 1102 56-57 0.7627537843857439 1102 58-59 0.8801524276428196 1102 60-61 0.6636612708239156 1102 62-63 0.7176400528345468 1102 64-65 0.944088203130633 1102 66-67 1.1789612888890346 1102 68-69 0.7968897636182035 1102 70-71 0.7048828002467431 1102 72-73 1.030142296710146 1102 74-75 0.8332413009594326 1102 76-77 1.057153719895414 1102 78-79 0.9068615262090205 1102 80-81 0.9115988025362611 1102 82-83 0.9762908249121409 1102 84-85 0.8415263951776701 1102 86-87 0.8904074808895075 1102 88-89 0.7720195315191845 1102 90-91 0.7337358280433506 1102 92-93 0.42263206613592885 1102 94-95 0.7643919500479512 1102 96-97 0.7023591140044836 1102 98-99 0.6754846176888698 1102 100-101 0.9821085390545861 1103 1 0.17442563477102624 1103 2 0.23213823807860123 1103 3 0.160215249341217 1103 4 0.19424496854144735 1103 5 0.3816150231548505 1103 6 0.2864648884601948 1103 7 0.1408150001726014 1103 8 0.14988006715510238 1103 9 0.2547671225028765 1103 10-11 0.3296197602552411 1103 12-13 0.344110785841913 1103 14-15 0.4071882015862087 1103 16-17 0.46662506576063834 1103 18-19 0.45444308263463995 1103 20-21 0.37464247532422235 1103 22-23 0.48855830738123274 1103 24-25 0.5386937931900277 1103 26-27 0.40089828041868003 1103 28-29 0.12451082125778612 1103 30-31 0.37865692786341754 1103 32-33 0.5404728992700214 1103 34-35 0.4311314839203746 1103 36-37 0.6310689226015072 1103 38-39 0.7331613536743902 1103 40-41 0.479369386991479 1103 42-43 0.596059077171951 1103 44-45 0.5221824426138255 1103 46-47 0.8885523497171093 1103 48-49 0.909806955628305 1103 50-51 0.7505751044866216 1103 52-53 1.1869706314986175 1103 54-55 1.2289133592673238 1103 56-57 1.372475008849925 1103 58-59 1.2112323777045546 1103 60-61 1.243116134574727 1103 62-63 1.378396310811766 1103 64-65 1.626521578079199 1103 66-67 1.5043342566593267 1103 68-69 1.6277848862668876 1103 70-71 1.4432177493668874 1103 72-73 1.4212161788530295 1103 74-75 1.39275747161442 1103 76-77 1.3310574840974816 1103 78-79 1.4852911209138888 1103 80-81 1.4570733207644295 1103 82-83 1.3185057547823433 1103 84-85 1.3466283292396177 1103 86-87 1.5291518401661008 1103 88-89 1.6127628167043966 1103 90-91 1.965870340552332 1103 92-93 2.1279648600986967 1103 94-95 2.151103207781059 1103 96-97 2.133911943258827 1103 98-99 2.27535244865199 1103 100-101 2.2865890345315485 1104 1 0.03803841016376808 1104 2 0.14161381698853148 1104 3 0.1926109802320184 1104 4 0.36945844214620394 1104 5 0.39424017693205826 1104 6 0.16924583351409694 1104 7 0.4646162637766693 1104 8 0.298322698732143 1104 9 0.6614030397770918 1104 10-11 0.4794958312522226 1104 12-13 0.11713926229540306 1104 14-15 0.2739908333084031 1104 16-17 0.17467518896384604 1104 18-19 0.34034674824769695 1104 20-21 0.5873812155668574 1104 22-23 0.24842454389510493 1104 24-25 0.18600056011277388 1104 26-27 0.0645172250443764 1104 28-29 0.2846895112756016 1104 30-31 0.14255131059536552 1104 32-33 0.05393089949501473 1104 34-35 0.12517381937423266 1104 36-37 0.397770306324162 1104 38-39 0.4356736830678898 1104 40-41 0.6206756676335203 1104 42-43 0.4177791318966797 1104 44-45 0.4877744530942749 1104 46-47 0.6205663139573332 1104 48-49 0.6600383018637999 1104 50-51 0.7390059782314538 1104 52-53 0.9310037131030455 1104 54-55 1.0691846603585446 1104 56-57 0.928947078554998 1104 58-59 0.7578227878133816 1104 60-61 1.0316838201325496 1104 62-63 1.045239586892194 1104 64-65 0.9324737991845069 1104 66-67 0.9982648201280284 1104 68-69 0.8443594378180848 1104 70-71 1.2941219048896286 1104 72-73 1.3679720695632511 1104 74-75 0.7135757296407697 1104 76-77 1.259205879552578 1104 78-79 1.1304059956603147 1104 80-81 1.3580190278855397 1104 82-83 1.1977266895300076 1104 84-85 1.338085756088848 1104 86-87 1.7132181630492376 1104 88-89 1.655942003889809 1104 90-91 1.5092799667331036 1104 92-93 1.4916030382153416 1104 94-95 1.508670134886131 1104 96-97 1.4910905715843157 1104 98-99 1.757813682280844 1104 100-101 2.123714026820007 1105 1 -0.03374812052024723 1105 2 0.21660942099767766 1105 3 0.12324224451898758 1105 4 0.08741166880349027 1105 5 -0.022719555791702817 1105 6 0.0363989898355328 1105 7 0.1010238600488691 1105 8 0.1823564599419214 1105 9 0.23569528524921424 1105 10-11 0.2522450839337722 1105 12-13 0.2295227685377128 1105 14-15 -0.059532992962033404 1105 16-17 -0.15966189561501665 1105 18-19 -0.047093905875740916 1105 20-21 -0.053971870418727974 1105 22-23 -0.23843144344444056 1105 24-25 0.03917446551105286 1105 26-27 0.09278344625063539 1105 28-29 -0.03834987680247082 1105 30-31 -0.1324179174686364 1105 32-33 -0.025328815644776626 1105 34-35 -0.015256108531524148 1105 36-37 -0.05877532406092456 1105 38-39 -0.05612779398504131 1105 40-41 -0.28571170708073623 1105 42-43 -0.11486110021164109 1105 44-45 -0.4298007183493766 1105 46-47 -0.1517872995471592 1105 48-49 -0.13240498985415172 1105 50-51 -0.040886759591650446 1105 52-53 -0.32954666495216145 1105 54-55 -0.13206380104465154 1105 56-57 0.12184315733114914 1105 58-59 0.19777355271579466 1105 60-61 -0.07296274220259846 1105 62-63 -0.0667043202644777 1105 64-65 0.2582606815477888 1105 66-67 0.05040127168421549 1105 68-69 0.09433745786380143 1105 70-71 0.06821193478236154 1105 72-73 0.2893937330061931 1105 74-75 0.2840654430221683 1105 76-77 0.29971493529373916 1105 78-79 0.32725846620718 1105 80-81 0.2034779693309403 1105 82-83 0.3689065734758472 1105 84-85 0.24869328202519014 1105 86-87 0.18558936051714525 1105 88-89 -0.25431384277803204 1105 90-91 -0.08453047814117198 1105 92-93 -0.20138096037794284 1105 94-95 -0.07968928132628506 1105 96-97 0.12486057334271194 1105 98-99 0.5502349940445157 1105 100-101 1.0081654071611332 1106 1 0.16919035514550274 1106 2 0.33965808950089027 1106 3 0.2756756696796856 1106 4 0.19048788551607032 1106 5 0.337932418496635 1106 6 0.1479692377694164 1106 7 0.40841596289551774 1106 8 0.3829992514937999 1106 9 0.7005713183070696 1106 10-11 0.4046785090944738 1106 12-13 0.542080160640559 1106 14-15 0.7351180630526528 1106 16-17 0.8333362678376872 1106 18-19 0.5594258370076375 1106 20-21 0.4244486989109362 1106 22-23 0.1440295299256249 1106 24-25 0.18667216982694157 1106 26-27 0.12595608169599615 1106 28-29 0.3142672030873328 1106 30-31 0.49235251411997183 1106 32-33 0.6217097518483357 1106 34-35 0.23910569128482706 1106 36-37 0.34377288530546224 1106 38-39 0.3722468616623402 1106 40-41 0.2817814058669157 1106 42-43 0.46720960685905766 1106 44-45 0.8432709069949809 1106 46-47 1.1870556756594581 1106 48-49 0.9567823379695355 1106 50-51 0.5486449208491209 1106 52-53 0.989782995286582 1106 54-55 1.0271418922519473 1106 56-57 0.9937439837774349 1106 58-59 1.104453993487141 1106 60-61 0.7066515828662645 1106 62-63 1.2262249726648164 1106 64-65 0.9030035649270403 1106 66-67 1.2020312074050565 1106 68-69 1.1956919114909823 1106 70-71 1.366306516967846 1106 72-73 1.4295452481577087 1106 74-75 0.8741710444914084 1106 76-77 1.0198710418134844 1106 78-79 1.2179839023871644 1106 80-81 1.0577020281004543 1106 82-83 1.1833005128697849 1106 84-85 0.9067511332648586 1106 86-87 1.2371274688734353 1106 88-89 1.4154519974423572 1106 90-91 1.3232978046871118 1106 92-93 1.7172370377855124 1106 94-95 1.5369314716581037 1106 96-97 1.604888121552083 1106 98-99 1.8704829279288155 1106 100-101 2.453528125251122 1107 1 0.1144197576038195 1107 2 0.08205799214175613 1107 3 -0.031601925549715304 1107 4 0.19018972316971627 1107 5 0.10694787425907748 1107 6 0.09232849297205092 1107 7 0.09982401936381535 1107 8 0.18149018492449187 1107 9 0.22603681666413422 1107 10-11 0.24397813182783068 1107 12-13 0.16826368260721125 1107 14-15 -0.18405255882881022 1107 16-17 0.027062688672550905 1107 18-19 0.21806341763317505 1107 20-21 0.17333819629440939 1107 22-23 0.2788293352073552 1107 24-25 0.3716149713360082 1107 26-27 0.1845936623215252 1107 28-29 0.3175622012618575 1107 30-31 0.18846727619859394 1107 32-33 -0.16464475709653215 1107 34-35 0.3435933388447623 1107 36-37 0.2960364851594264 1107 38-39 0.46741845713514607 1107 40-41 -0.055339806254295354 1107 42-43 -0.2464328417231485 1107 44-45 0.2766863870972074 1107 46-47 -0.01171212280713263 1107 48-49 -0.36525125093151445 1107 50-51 -0.7834288286945039 1107 52-53 -0.46431217326641416 1107 54-55 -0.3094012500140835 1107 56-57 -0.0744925989169829 1107 58-59 -0.2201991645997552 1107 60-61 -0.3194802170607076 1107 62-63 -0.4117481318745533 1107 64-65 -0.36596016879573057 1107 66-67 -0.5226645453027459 1107 68-69 -0.24159055169509003 1107 70-71 -0.02692398078071534 1107 72-73 -0.20291185297408987 1107 74-75 -0.17262827399465763 1107 76-77 -0.2927947729607645 1107 78-79 -0.5452995343902529 1107 80-81 -0.2624908646827997 1107 82-83 0.2178660477365284 1107 84-85 0.03259998321009405 1107 86-87 -0.15757254329645676 1107 88-89 -0.06326286199539766 1107 90-91 -0.02023694035365864 1107 92-93 -0.15379557752421036 1107 94-95 -0.17830523273522658 1107 96-97 -0.1517748941470778 1107 98-99 -0.03066712683569861 1107 100-101 0.2184636248251799 1108 1 -0.10888702886408552 1108 2 -0.04794677268024472 1108 3 -0.06347858472880574 1108 4 0.024891241105862605 1108 5 0.0820686965312234 1108 6 -0.08161473757726867 1108 7 -0.048355137943381976 1108 8 -0.09688433008469843 1108 9 -0.2063367453806535 1108 10-11 -0.4101434392020522 1108 12-13 0.014815993545880701 1108 14-15 -0.3397192935643645 1108 16-17 -0.03971629165554447 1108 18-19 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-3.03855569611288 2316 68-69 -3.552392379696755 2316 70-71 -3.537829672513162 2316 72-73 -3.1514369879969415 2316 74-75 -2.3249755943104056 2316 76-77 -2.29615995783821 2316 78-79 -3.205457407261047 2316 80-81 -2.165295185414003 2316 82-83 -2.221062078561772 2316 84-85 -2.205788051688117 2316 86-87 -3.2704308731676655 2316 88-89 -4.256387083017415 2316 90-91 -3.980768722655281 2316 92-93 -3.8261885978815044 2316 94-95 -4.067648813956662 2316 96-97 -5.354620760370597 2316 98-99 -5.948012369876832 2316 100-101 -5.984132968444452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 557.0 3 165.0 4 131.0 5 146.0 6 173.0 7 201.0 8 259.0 9 336.0 10 453.0 11 678.0 12 1029.0 13 1455.0 14 1754.0 15 2347.0 16 3231.0 17 4399.0 18 3628.0 19 2260.0 20 2286.0 21 2519.0 22 2707.0 23 2985.0 24 3306.0 25 3827.0 26 4462.0 27 5055.0 28 5985.0 29 7137.0 30 8859.0 31 10568.0 32 13360.0 33 17535.0 34 23354.0 35 33013.0 36 46277.0 37 70126.0 38 72375.0 39 12519.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.96178103809508 24.355432036926324 16.438031529566373 21.244755395412216 2 13.307663309817439 24.612256987452206 39.90656470461522 22.173514998115138 3 16.211791568834414 30.565667880145618 32.53559657928182 20.68694397173815 4 9.73173884938591 20.385692738633914 43.228291316526615 26.654277095453565 5 9.036548608922612 41.39589881996127 37.44151656929529 12.12603600182083 6 22.678632510861103 43.76250744049623 19.325628435758553 14.233231612884111 7 21.76526833209885 36.35122213992324 25.930644401050433 15.95286512692748 8 21.557500673309992 35.273094532722865 23.401292755184485 19.768112038782657 9 23.388382565229566 18.80857733824575 24.975761101415554 32.827278995109126 10-11 20.176771145605233 29.20898750508389 30.755836033517298 19.858405315793583 12-13 22.58152697187072 28.944111757632236 29.701578358404916 18.77278291209213 14-15 20.5359739742112 30.4572287274725 28.89929010783036 20.107507190485936 16-17 16.399334756290525 33.21330218089511 30.4074281058734 19.979934956940955 18-19 16.07786628599902 32.898977180141536 33.25895051545004 17.76420601840941 20-21 17.51710341745262 31.270781897436034 32.31879531631562 18.89331936879572 22-23 17.695026151578183 30.353332390934117 32.717138875987 19.234502581500703 24-25 18.004864869958283 31.270721801859523 32.26967586827968 18.454737459902514 26-27 17.144968741418026 30.899417906509647 33.83842463208568 18.117188719986647 28-29 16.51154713892643 30.898759941200858 34.09300171766721 18.496691202205508 30-31 17.11336847716638 31.129080893636957 32.8698044832618 18.887746145934855 32-33 16.570510922179803 32.16564453514104 32.861169591273026 18.402674951406127 34-35 17.700825729816465 30.869425919893168 32.824846740561455 18.60490160972891 36-37 17.811877816024026 30.16004641601837 32.893449122093784 19.13462664586382 38-39 16.8439587422359 31.764897515218582 32.9992111377655 18.39193260478002 40-41 16.251272028299603 31.686023033807686 33.98738470981602 18.075320228076695 42-43 16.659913878393276 31.6572957707803 31.625393893953852 20.057396456872567 44-45 16.737604690793674 31.35124148272585 32.08808160430313 19.823072222177355 46-47 17.73277804549089 31.410734428294912 32.00851237956275 18.847975146651446 48-49 17.331328601484167 31.463072866259527 31.839969632889087 19.36562889936722 50-51 16.802056762886735 31.3920165833238 32.17285961381854 19.633067039970925 52-53 17.192200782075528 32.488036667362145 31.23375443366828 19.086008116894043 54-55 17.217585612750376 31.15873357742839 31.779426017660995 19.84425479216024 56-57 17.861292142167677 31.59139648875289 30.539718610202026 20.0075927588774 58-59 17.82865696797061 30.723336775037723 31.452421690006986 19.995584566984675 60-61 17.98459463316004 32.0844629794658 31.193444776794486 18.73749761057968 62-63 17.469255123248725 31.878829648614598 31.88973304185826 18.762182186278416 64-65 17.445532346773128 31.363353694485795 31.762455614213142 19.42865834452793 66-67 17.270919793827478 31.24867911473428 30.722478808625432 20.75792228281281 68-69 16.749886579493303 31.268249931678447 31.924406073684963 20.057457415143286 70-71 18.333120200300183 30.877592090296613 31.338767103925914 19.450520605477294 72-73 17.487525255720477 30.098492262091447 32.03333957014461 20.380642912043463 74-75 16.83557883996441 31.932948440619146 31.138215431335148 20.093257288081293 76-77 17.202090812781258 30.4920517614815 32.086007088733005 20.219850337004235 78-79 16.888732140309124 30.28185129645135 32.15000121151097 20.679415351728554 80-81 17.91484595647527 31.25981029613296 30.511602736517325 20.313741010874452 82-83 17.7848075706382 30.26720691372649 31.709585795630463 20.238399720004846 84-85 17.002915985686627 30.087560345825672 31.15338946314092 21.75613420534678 86-87 17.172955852085575 30.864907139559207 31.44359386126326 20.51854314709196 88-89 17.81869676945174 29.406483172853648 31.06947718128304 21.705342876411574 90-91 17.10807045410186 28.367535115998898 32.61712624260495 21.907268187294303 92-93 18.009520783633732 29.97081298229931 31.754209769573343 20.265456464493617 94-95 17.12927626132293 28.44890832751117 32.23311269689346 22.188702714272445 96-97 16.372552846929338 28.724256346814244 32.4628156580104 22.44037514824602 98-99 16.698620251392136 29.711179087276367 31.356429671596135 22.233770989735362 100-101 16.33736893061385 28.99947619374637 32.0630021773643 22.60015269827548 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 718.0 1 493.0 2 317.5 3 371.5 4 323.5 5 334.0 6 567.5 7 992.0 8 1481.0 9 1673.0 10 1396.0 11 1129.5 12 1087.5 13 1077.0 14 1047.0 15 981.0 16 918.5 17 860.5 18 833.0 19 844.5 20 870.0 21 949.0 22 1046.5 23 1169.0 24 1251.5 25 1452.0 26 2208.5 27 3075.0 28 3361.0 29 4233.0 30 6416.0 31 8298.0 32 11414.5 33 15820.0 34 18832.5 35 20925.5 36 24110.5 37 26166.0 38 24562.0 39 22102.0 40 19239.5 41 16448.5 42 13536.0 43 12675.0 44 12686.0 45 11264.0 46 9596.5 47 9990.5 48 15973.5 49 14857.0 50 6902.0 51 3096.0 52 1571.0 53 1121.5 54 945.5 55 839.5 56 757.5 57 695.0 58 667.5 59 617.5 60 523.5 61 493.5 62 414.0 63 299.5 64 218.0 65 173.0 66 127.0 67 79.0 68 58.0 69 39.5 70 37.0 71 34.0 72 20.5 73 16.5 74 16.5 75 18.5 76 17.0 77 10.0 78 7.5 79 7.0 80 6.0 81 5.0 82 3.5 83 2.0 84 0.5 85 1.5 86 2.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03365108935306454 2 0.021267488471136787 3 0.02019065361183872 4 0.04818835995358842 5 0.05384174296490326 6 0.04845756866841293 7 0.049534403527711 8 0.04280418565709809 9 0.04172735079780003 10-11 0.050611238387009067 12-13 0.04186195515521228 14-15 0.0363431765013097 16-17 0.04482325101828196 18-19 0.04886138174064971 20-21 0.011306766022629684 22-23 0.017902379535830334 24-25 0.005518778653902584 26-27 0.011037557307805168 28-29 0.006730217870612907 30-31 0.008883887589209037 32-33 0.003499713292718712 34-35 0.00807626144473549 36-37 0.009556909376270328 38-39 0.010229931163331618 40-41 0.0013460435741225814 42-43 0.0020190653611838723 44-45 0.0021536697185961305 46-47 0.0 48-49 0.0012114392167103232 50-51 4.0381307223677444E-4 52-53 0.0030959002204819373 54-55 0.005114965581665809 56-57 0.014133457528287105 58-59 0.009287700661445812 60-61 0.00807626144473549 62-63 0.004038130722367745 64-65 0.0 66-67 0.006057196083551616 68-69 0.014402666243111623 70-71 0.004441943794604519 72-73 0.0022882740760083887 74-75 0.0036343176501309705 76-77 0.00659561351320065 78-79 0.005922591726139358 80-81 0.010229931163331618 82-83 0.006461009155788391 84-85 0.015614105459821944 86-87 0.003903526364955486 88-89 0.005518778653902584 90-91 0.0030959002204819373 92-93 0.016421731604295494 94-95 0.009018491946621295 96-97 0.00874928323179678 98-99 0.023421158189732918 100-101 0.037285407003195506 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 371459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.102083944661455 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81809537839322 14.63795465986825 2 9.287407427247524 3.733924874616041 3 3.8582582260850935 2.3267709222282944 4 2.098538924080299 1.687400224520068 5 1.489199287541348 1.4968004544243105 6 1.040564610089593 1.255051028511895 7 0.8678067790708575 1.221130730444006 8 0.6454982523335699 1.0380688043633348 9 0.591929932637838 1.0709122675719258 >10 5.579140496310482 24.32435342796917 >50 1.0392254020971996 14.671874957936136 >100 0.6441590443411767 23.47849964599054 >500 0.02812336784025927 3.9468689680422337 >1k 0.012052871931539687 5.110389033513793 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3419 0.9204245959850212 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2692 0.7247098603075979 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2669 0.718518059866634 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2343 0.6307560188338417 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2035 0.5478397346678906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1774 0.4775762600986919 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1761 0.47407654680597316 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1267 0.34108744168266214 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1023 0.2754005152654802 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 930 0.25036410478680016 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 903 0.2430954694865382 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 836 0.22505848559329564 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 808 0.21752064157820916 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 804 0.21644380671891109 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 727 0.19571473567742337 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 717 0.1930226485291782 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 701 0.18871530909198592 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 700 0.18844610037716142 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 663 0.17848537792865432 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 658 0.17713933435453172 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 655 0.17633170821005817 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 655 0.17633170821005817 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 654 0.17606249949523367 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 650 0.1749856646359356 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 648 0.17444724720628654 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 626 0.1685246554801472 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 620 0.16690940319120012 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 587 0.15802551560199107 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 580 0.15614105459821948 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 539 0.14510349729041427 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 486 0.13083543540471493 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 485 0.1305662266898904 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 482 0.12975860054541685 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 477 0.12841255697129428 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 463 0.12464363496375104 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 462 0.12437442624892653 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 454 0.1222207565303304 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 438 0.11791341709313813 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 435 0.11710579094866458 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 427 0.11495212123006845 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 421 0.11333686894112137 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 421 0.11333686894112137 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 419 0.11279845151147233 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 417 0.11226003408182329 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 399 0.10741427721498199 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 398 0.10714506850015748 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 397 0.10687585978533297 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 391 0.10526060749638587 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 390 0.10499139878156136 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 384 0.10337614649261426 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 383 0.10310693777778975 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 374 0.10068405934436911 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3460435741225816E-4 0.0 20-21 0.0 0.0 0.0 2.692087148245163E-4 0.0 22-23 0.0 0.0 0.0 6.730217870612907E-4 0.0 24-25 0.0 0.0 0.0 0.0013460435741225814 0.0 26-27 0.0 0.0 0.0 0.002961295863069679 0.0 28-29 0.0 0.0 0.0 0.006461009155788391 0.0 30-31 0.0 0.0 0.0 0.011575974737454201 0.0 32-33 0.0 0.0 0.0 0.01911381875254066 0.0 34-35 0.0 0.0 0.0 0.028805332486223245 0.0 36-37 0.0 0.0 0.0 0.04011209850885293 0.0 38-39 0.0 0.0 0.0 0.05330332553525423 0.0 40-41 0.0 0.0 0.0 0.06905203535248844 0.0 42-43 0.0 0.0 0.0 0.10404916827967556 0.0 44-45 0.0 0.0 0.0 0.13837327941980138 0.0 46-47 0.0 0.0 0.0 0.16825544676532267 0.0 48-49 0.0 0.0 0.0 0.20554085376851822 0.0 50-51 0.0 0.0 0.0 0.2636899361706137 0.0 52-53 0.0 0.0 0.0 0.37029658724112213 0.0 54-55 0.0 0.0 0.0 0.44971315811435447 0.0 56-57 0.0 0.0 0.0 0.5923937769713481 0.0 58-59 0.0 0.0 0.0 0.7728982202611863 0.0 60-61 0.0 0.0 0.0 0.9367117232319044 0.0 62-63 0.0 0.0 0.0 1.1569244519583588 0.0 64-65 0.0 0.0 0.0 1.2868176568611878 0.0 66-67 0.0 0.0 0.0 1.5747363773660081 0.0 68-69 0.0 0.0 0.0 1.9049208660982773 0.0 70-71 0.0 0.0 0.0 2.1249989904673194 0.0 72-73 0.0 0.0 0.0 2.5146786051758068 0.0 74-75 0.0 0.0 0.0 2.910146207253021 0.0 76-77 0.0 0.0 0.0 3.127397640116406 0.0 78-79 0.0 0.0 0.0 3.367935626812111 0.0 80-81 0.0 0.0 0.0 3.826128859443438 0.0 82-83 0.0 0.0 0.0 4.193464150821491 0.0 84-85 0.0 0.0 0.0 4.76930159183113 0.0 86-87 0.0 0.0 0.0 5.579619823452925 0.0 88-89 0.0 0.0 0.0 6.6719341838534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAAA 15 6.598803E-4 95.05631 1 GTATGGT 55 8.142233E-7 51.8489 1 ATGTATT 50 2.8133949E-5 47.52816 1 GGTACTC 60 1.4876005E-6 47.508957 8 TGGGATA 110 1.6079866E-9 38.876198 6 AGGGTCT 55 0.002569905 34.565933 5 AATGGTG 85 1.6226433E-5 33.549286 5 GGGTAGG 130 8.20728E-9 32.90411 1 CATGGGG 435 0.0 32.77804 4 CCCGCGC 30 0.0039490187 31.668371 88-89 CACTGAA 30 0.003954228 31.659843 42-43 CATGGGT 195 1.6370905E-11 29.248098 4 CCCAATG 65 0.005822156 29.248098 2 GGGATAG 65 0.0058298283 29.240217 7 GGATATG 100 4.924264E-5 28.516895 1 GTACATG 1740 0.0 27.315033 1 GTGGTAC 105 6.878305E-5 27.151632 6 TACAATC 105 6.878305E-5 27.151632 7 ACAATCA 105 6.8846166E-5 27.147974 8 CCGATGA 35 0.008393 27.140665 22-23 >>END_MODULE