##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 371459 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.863489644886783 31.0 31.0 34.0 30.0 34.0 2 32.0111156278351 31.0 31.0 34.0 30.0 34.0 3 32.0929577692289 33.0 31.0 34.0 30.0 34.0 4 35.85344815982383 37.0 35.0 37.0 35.0 37.0 5 35.63187054291321 37.0 35.0 37.0 33.0 37.0 6 35.76534153163606 37.0 35.0 37.0 33.0 37.0 7 35.707596262306204 37.0 35.0 37.0 33.0 37.0 8 35.70084719982555 37.0 35.0 37.0 33.0 37.0 9 37.320896788070826 39.0 37.0 39.0 34.0 39.0 10-11 37.19040863190823 39.0 37.0 39.0 33.0 39.0 12-13 37.13785370660019 39.0 37.0 39.0 33.0 39.0 14-15 38.14875262141986 40.0 37.0 41.0 33.0 41.0 16-17 38.09076506424666 40.0 37.0 41.0 33.0 41.0 18-19 38.32990451166885 40.0 38.0 41.0 33.5 41.0 20-21 38.3957543093585 40.0 38.0 41.0 34.0 41.0 22-23 38.281332529296634 40.0 38.0 41.0 34.0 41.0 24-25 38.14571056294234 40.0 37.0 41.0 33.0 41.0 26-27 37.90260163302007 40.0 37.0 41.0 33.0 41.0 28-29 37.84416180520596 40.0 37.0 41.0 33.0 41.0 30-31 37.77833219816992 39.5 37.0 41.0 33.0 41.0 32-33 37.557719694501955 39.0 36.0 41.0 33.0 41.0 34-35 37.36327966208922 39.0 36.0 41.0 33.0 41.0 36-37 37.099245138763635 39.0 35.0 41.0 31.5 41.0 38-39 36.92396603662854 39.0 35.0 41.0 31.0 41.0 40-41 36.76600378507453 39.0 35.0 41.0 31.0 41.0 42-43 36.49436815368587 39.0 35.0 40.0 31.0 41.0 44-45 36.19714961812744 39.0 35.0 40.0 30.0 41.0 46-47 35.773948134249004 39.0 35.0 40.0 29.5 41.0 48-49 35.36528257492752 39.0 35.0 40.0 27.0 41.0 50-51 35.072067172958526 38.0 35.0 40.0 26.5 41.0 52-53 34.881677385660325 38.0 34.5 40.0 26.0 41.0 54-55 34.66855292239521 38.0 34.0 40.0 24.5 41.0 56-57 34.3745473928482 38.0 34.0 40.0 23.5 41.0 58-59 34.093698093194675 38.0 33.5 40.0 23.0 41.0 60-61 33.75290139692402 38.0 33.0 40.0 22.0 41.0 62-63 33.40259221071504 37.0 33.0 40.0 21.5 41.0 64-65 33.00428849482715 37.0 32.5 39.0 19.5 40.0 66-67 32.544050622006736 36.0 31.5 39.0 18.5 40.0 68-69 32.230298633227356 36.0 31.5 39.0 16.5 40.0 70-71 31.76775364172089 35.0 31.0 38.0 12.0 40.0 72-73 31.25602960219028 35.0 31.0 37.5 8.0 39.5 74-75 30.680679428954473 35.0 30.0 37.0 6.5 39.0 76-77 29.17782043240304 33.0 28.5 35.5 2.0 38.0 78-79 29.92784802629631 34.0 29.5 36.0 2.0 38.0 80-81 29.653704715729056 34.0 29.0 36.0 2.0 37.5 82-83 29.269075456510677 34.0 29.0 35.0 2.0 37.0 84-85 28.792993304779262 34.0 29.0 35.0 2.0 36.5 86-87 28.460862437038813 34.0 29.0 35.0 2.0 36.0 88-89 28.01462072530212 33.5 28.5 35.0 2.0 36.0 90-91 27.520237765136933 33.0 26.5 35.0 2.0 35.0 92-93 27.05885575527851 33.0 26.0 35.0 2.0 35.0 94-95 26.490164459603886 32.5 25.0 34.0 2.0 35.0 96-97 25.7543537779405 32.0 24.0 34.0 2.0 35.0 98-99 24.942915638065035 32.0 19.0 34.0 2.0 35.0 100-101 21.864744157497867 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09128201941372183 1101 2 0.1492758719835976 1101 3 0.21676879747352729 1101 4 0.18849175088246994 1101 5 0.241702079093848 1101 6 0.13077093150462105 1101 7 0.10700817876066537 1101 8 0.11148451616824673 1101 9 0.2457194185370568 1101 10-11 0.1307078031959179 1101 12-13 0.10795159751423 1101 14-15 0.28421437647919134 1101 16-17 0.24980730515915894 1101 18-19 0.22760305144196025 1101 20-21 0.10063625560112399 1101 22-23 0.15473966217351887 1101 24-25 0.21196588929355187 1101 26-27 -0.15437087055723708 1101 28-29 0.08024266123615575 1101 30-31 0.36438872689529944 1101 32-33 0.4556642408454934 1101 34-35 0.37437332192368444 1101 36-37 0.46866240172284535 1101 38-39 0.49509222749995985 1101 40-41 0.6078032079698588 1101 42-43 0.9153731900709019 1101 44-45 0.9079655607906716 1101 46-47 0.7675889349740928 1101 48-49 0.7328114176726359 1101 50-51 0.9569609136379071 1101 52-53 0.8312229550028363 1101 54-55 0.8606050239502849 1101 56-57 0.8855697650918088 1101 58-59 0.8852787129548787 1101 60-61 0.9503766794221065 1101 62-63 0.9924990296209515 1101 64-65 1.0825218513896075 1101 66-67 1.0969489081625383 1101 68-69 0.9676555898970562 1101 70-71 0.7467359767726798 1101 72-73 0.8767598885322094 1101 74-75 1.389403879312166 1101 76-77 0.9988325434437719 1101 78-79 0.8124120728721245 1101 80-81 0.9126096147287797 1101 82-83 1.3017573305734054 1101 84-85 1.3424761707644848 1101 86-87 1.528754994735987 1101 88-89 0.9832031722886292 1101 90-91 0.9736606215650774 1101 92-93 0.789985625481382 1101 94-95 1.026251326288616 1101 96-97 1.413203670628409 1101 98-99 1.7841918170834354 1101 100-101 2.2472698628338144 1102 1 0.09987396694464934 1102 2 0.0816322563825338 1102 3 0.011243250977742036 1102 4 0.010275589805708307 1102 5 0.07857146095285827 1102 6 0.07947255473231962 1102 7 0.12743031380828285 1102 8 0.16353939509367166 1102 9 0.2229730076434251 1102 10-11 0.14744669699123136 1102 12-13 0.28171603438224224 1102 14-15 0.2726905552190928 1102 16-17 0.17538255487333032 1102 18-19 0.3394866391106319 1102 20-21 0.24623040075368863 1102 22-23 0.2468805974977215 1102 24-25 0.25963921728686756 1102 26-27 0.28629334517937366 1102 28-29 -0.02772149220027842 1102 30-31 0.21462697372723483 1102 32-33 0.2951796686970951 1102 34-35 0.4063090089714052 1102 36-37 0.45935160599211855 1102 38-39 0.39132135522901734 1102 40-41 0.319726503443853 1102 42-43 0.5224576102342482 1102 44-45 0.6633791723524922 1102 46-47 0.7304878101478138 1102 48-49 0.5771961863062103 1102 50-51 0.554601037690567 1102 52-53 0.6228939005278349 1102 54-55 0.5526213589198576 1102 56-57 0.6773434032167813 1102 58-59 0.8281302810708766 1102 60-61 0.9333434001735412 1102 62-63 0.7160933390816808 1102 64-65 0.716612713802057 1102 66-67 0.8897426261138861 1102 68-69 0.9931165085165929 1102 70-71 1.1446954748112468 1102 72-73 1.1346620840999968 1102 74-75 0.9209904115199876 1102 76-77 0.6427391068701489 1102 78-79 1.1836663602881714 1102 80-81 1.6030924068721113 1102 82-83 1.5934984493750513 1102 84-85 1.4483642221381707 1102 86-87 1.2326734020422236 1102 88-89 1.2105611655383015 1102 90-91 1.4169486332309553 1102 92-93 1.4908472163951387 1102 94-95 1.2055217980584167 1102 96-97 1.4496800821381974 1102 98-99 1.4153781904600358 1102 100-101 1.6398585378938897 1103 1 0.059846626436758754 1103 2 0.08964260671766766 1103 3 0.07390671861899989 1103 4 0.08199908799578992 1103 5 -0.02195372788496286 1103 6 -0.030949618977551552 1103 7 -0.09379066305690742 1103 8 0.006439581316008969 1103 9 0.044077508365298 1103 10-11 -0.1356879759339904 1103 12-13 -0.007488301041156831 1103 14-15 -0.2221639193479632 1103 16-17 -0.06554172168427641 1103 18-19 -0.22382895544079418 1103 20-21 -0.3502843819520507 1103 22-23 -0.12266437833522303 1103 24-25 0.002390844116838764 1103 26-27 0.027908109053633723 1103 28-29 0.12682909820124877 1103 30-31 -0.0036106097066550547 1103 32-33 -0.06531399486122069 1103 34-35 0.3806825137873773 1103 36-37 0.15155467900941488 1103 38-39 0.07078179867531986 1103 40-41 0.22042884761341952 1103 42-43 0.20753610957514468 1103 44-45 0.07086433384980495 1103 46-47 0.10544857791391848 1103 48-49 0.09408348343196593 1103 50-51 0.5189886312376402 1103 52-53 0.17050613958988237 1103 54-55 0.18351229679691983 1103 56-57 0.17252742854859804 1103 58-59 0.6676582388325798 1103 60-61 0.7021068699071833 1103 62-63 0.5536387612902658 1103 64-65 0.1308928982109947 1103 66-67 0.5688926989986598 1103 68-69 0.8755279134141745 1103 70-71 0.5651307608055127 1103 72-73 0.5071590675771205 1103 74-75 0.9137699368273928 1103 76-77 0.24056511801558855 1103 78-79 0.49152954615480127 1103 80-81 0.35624842213258034 1103 82-83 0.556754410930953 1103 84-85 0.7344753138753397 1103 86-87 0.5895733929752183 1103 88-89 0.5556932299558852 1103 90-91 0.771930603839909 1103 92-93 0.8924754412847662 1103 94-95 0.8102945300865692 1103 96-97 0.34518982219413274 1103 98-99 0.6531534263765373 1103 100-101 0.3812762687712272 1104 1 0.1609857571555473 1104 2 0.2675741717794864 1104 3 0.2079133451009767 1104 4 0.21290658216224045 1104 5 0.01544633961370323 1104 6 0.1112112818756188 1104 7 0.2728359640782827 1104 8 0.05892886680996412 1104 9 7.767510012328671E-4 1104 10-11 0.0839548617680066 1104 12-13 0.07240689457796634 1104 14-15 0.3729202906817548 1104 16-17 0.45597619993743166 1104 18-19 0.36107602022702423 1104 20-21 0.11149540546344383 1104 22-23 0.22490113347523533 1104 24-25 -0.1466977424393221 1104 26-27 0.03828330728560303 1104 28-29 0.17204050329848286 1104 30-31 -0.10422535562498325 1104 32-33 -0.21217258515519433 1104 34-35 -0.3702238191116862 1104 36-37 -0.3728228635310771 1104 38-39 0.03898121731583615 1104 40-41 0.20524346216338074 1104 42-43 -0.020063224147698122 1104 44-45 0.1962539934895915 1104 46-47 0.26585952140737135 1104 48-49 0.15225209219736513 1104 50-51 0.23753733733175153 1104 52-53 0.2503305704781482 1104 54-55 0.3942315204896829 1104 56-57 0.6243820088411667 1104 58-59 0.5189760958587968 1104 60-61 0.36188093082291317 1104 62-63 0.5799795714919966 1104 64-65 0.714306371089954 1104 66-67 0.39408566897626685 1104 68-69 0.5818325065895493 1104 70-71 0.5239729046502539 1104 72-73 0.4941819046259077 1104 74-75 0.5245421433442807 1104 76-77 0.20307432650300328 1104 78-79 0.35055712988399534 1104 80-81 0.7468352613442235 1104 82-83 0.8804322898760866 1104 84-85 0.9262912820275808 1104 86-87 0.6987397844457099 1104 88-89 0.7790575667089108 1104 90-91 0.8650388448464383 1104 92-93 0.9795995263330859 1104 94-95 1.3640212911304026 1104 96-97 1.289695757359123 1104 98-99 1.4871067071383273 1104 100-101 1.5343222368145994 1105 1 -0.012963601029788663 1105 2 0.12070411726919872 1105 3 -0.006698928505500135 1105 4 -0.05858981312525913 1105 5 -0.02899712794388165 1105 6 0.00874073501435646 1105 7 -0.04991768459412782 1105 8 -0.03466177852149599 1105 9 -0.10428743046526989 1105 10-11 -0.09808311959299232 1105 12-13 -0.21616792518640437 1105 14-15 -0.01900427411514727 1105 16-17 0.0612162210381868 1105 18-19 0.0918215802762603 1105 20-21 0.10474755949896064 1105 22-23 0.016201467570532202 1105 24-25 -0.015411475514753192 1105 26-27 -0.05682395489129277 1105 28-29 0.05875581893062787 1105 30-31 0.11759634298589106 1105 32-33 0.0021099891370468526 1105 34-35 0.18437438732404132 1105 36-37 -0.057388539634473545 1105 38-39 0.20835874501057816 1105 40-41 0.0734516562903309 1105 42-43 0.5455689193374482 1105 44-45 0.5576923616977822 1105 46-47 0.22124021421552698 1105 48-49 0.40510948625398413 1105 50-51 0.0281562928443293 1105 52-53 0.05941268758734708 1105 54-55 -0.1656418749736588 1105 56-57 -0.06774477153513203 1105 58-59 -0.2775334874012785 1105 60-61 0.18067818772461663 1105 62-63 0.1748670341260734 1105 64-65 0.2917417500243715 1105 66-67 0.6574275012252571 1105 68-69 0.7369450439554726 1105 70-71 0.5833187812108314 1105 72-73 0.2901200090746485 1105 74-75 -0.44422434162143176 1105 76-77 -0.1611335159446874 1105 78-79 0.5361119039300668 1105 80-81 0.7821570478749038 1105 82-83 0.5829336086733399 1105 84-85 0.47702101650973106 1105 86-87 0.7129608148659656 1105 88-89 0.8281607845742158 1105 90-91 0.8733912274734799 1105 92-93 0.699179269607221 1105 94-95 0.4332361671634608 1105 96-97 0.8914409657290889 1105 98-99 1.2788686202735455 1105 100-101 1.234273880915179 1106 1 0.2021420004394514 1106 2 0.21964810257681933 1106 3 0.3757620448645653 1106 4 0.2983890665808886 1106 5 0.061223257731043645 1106 6 0.028254049982216145 1106 7 0.16312180117263608 1106 8 0.19169267510568488 1106 9 0.2210110084695316 1106 10-11 0.2121785883959859 1106 12-13 0.23286788748576726 1106 14-15 0.4330159279050534 1106 16-17 0.30008215308593833 1106 18-19 0.15105023041400045 1106 20-21 0.24707997455865183 1106 22-23 0.07370835462287317 1106 24-25 0.1853461002428176 1106 26-27 0.30692719295077353 1106 28-29 0.30072092453209365 1106 30-31 0.2800159940419036 1106 32-33 0.2876930929019679 1106 34-35 0.2646085471036628 1106 36-37 0.37454442638939867 1106 38-39 0.49922192223738904 1106 40-41 0.40839196850336634 1106 42-43 0.5425845299894192 1106 44-45 0.5504609567391086 1106 46-47 0.9404780470897265 1106 48-49 1.0333234440133978 1106 50-51 1.0344694241574572 1106 52-53 1.2146009336846788 1106 54-55 1.1221221286994378 1106 56-57 1.0836555957743172 1106 58-59 1.2453085695316943 1106 60-61 1.1988140922241612 1106 62-63 0.8991553720507781 1106 64-65 1.2528299042943374 1106 66-67 1.3120418262941271 1106 68-69 1.4722526656267263 1106 70-71 1.3416980282264426 1106 72-73 1.059059403014043 1106 74-75 1.7760465491040058 1106 76-77 1.376860056139794 1106 78-79 1.4800972115517368 1106 80-81 1.1707015841467125 1106 82-83 1.2058674929708637 1106 84-85 1.0926087116428818 1106 86-87 1.2818313382819433 1106 88-89 1.351806330947035 1106 90-91 1.1427897582356472 1106 92-93 1.1542986451811394 1106 94-95 1.5849808870899977 1106 96-97 1.5348064386400182 1106 98-99 1.783689556912666 1106 100-101 1.7456375172788157 1107 1 0.02943906749555225 1107 2 0.03254818776606072 1107 3 0.018064588371927925 1107 4 0.0031048996099798387 1107 5 0.12467707299209252 1107 6 0.08662085051768997 1107 7 0.022839459685471297 1107 8 -0.024560215243589312 1107 9 0.04985973671585242 1107 10-11 -0.21256584387856492 1107 12-13 -0.09874036370079864 1107 14-15 0.012129351295591562 1107 16-17 -0.04088474480867177 1107 18-19 -0.023119174478296145 1107 20-21 0.015429128751179633 1107 22-23 0.04880875622930603 1107 24-25 -0.05744323893205916 1107 26-27 -0.11441879706992353 1107 28-29 -0.23413973020807788 1107 30-31 -0.28510205390747245 1107 32-33 -0.1822198923724656 1107 34-35 -0.11612347188137306 1107 36-37 -0.012628507771488273 1107 38-39 -0.26928239807416077 1107 40-41 -0.19875784286330855 1107 42-43 0.07863174916916904 1107 44-45 -0.07935832036933022 1107 46-47 0.1259350265226118 1107 48-49 0.08544404764001712 1107 50-51 0.3328112764149793 1107 52-53 0.3148990960310343 1107 54-55 0.29482810411625593 1107 56-57 -0.06228574534774367 1107 58-59 -0.034224715122071814 1107 60-61 0.25785632174831363 1107 62-63 0.36869109068288 1107 64-65 0.4459113208697332 1107 66-67 0.10198341081402162 1107 68-69 0.14010893920588785 1107 70-71 0.13111326493083553 1107 72-73 0.20238725989611694 1107 74-75 -0.09243951637001047 1107 76-77 -0.2541883800291238 1107 78-79 -0.03695889164894339 1107 80-81 0.36485395119064634 1107 82-83 0.03089487522715828 1107 84-85 0.1983194333174616 1107 86-87 0.3136403926813607 1107 88-89 0.23273611067199695 1107 90-91 0.02165369884657764 1107 92-93 0.5411338648476836 1107 94-95 0.40662808192996636 1107 96-97 0.8064140813319973 1107 98-99 1.2637187646527188 1107 100-101 1.497499510707076 1108 1 -0.14394599819092235 1108 2 0.08987580331299938 1108 3 -0.05738296455162839 1108 4 -0.11793519296370647 1108 5 -0.10261855017856192 1108 6 -0.1789022588690088 1108 7 -0.16216496479209752 1108 8 -0.07097638841083409 1108 9 -0.14010010264158268 1108 10-11 -0.013617508162816705 1108 12-13 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2316 84-85 -2.2493540191129533 2316 86-87 -2.3663139072770925 2316 88-89 -1.9356893151692987 2316 90-91 -2.655163916442163 2316 92-93 -2.5796181085631993 2316 94-95 -2.2804928154526714 2316 96-97 -2.342692690583249 2316 98-99 -2.405599889273333 2316 100-101 -2.331478470181999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 16.0 6 28.0 7 61.0 8 134.0 9 270.0 10 422.0 11 751.0 12 1093.0 13 1567.0 14 2019.0 15 2797.0 16 4046.0 17 5272.0 18 4355.0 19 2716.0 20 2429.0 21 2393.0 22 2753.0 23 3083.0 24 3452.0 25 4084.0 26 4659.0 27 5620.0 28 7013.0 29 8540.0 30 10244.0 31 12597.0 32 15670.0 33 20135.0 34 26335.0 35 35906.0 36 50403.0 37 75042.0 38 52753.0 39 2798.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.63592298370809 24.35896054934698 17.039719940756697 18.965396526188233 2 14.334327651005266 25.9767286589634 39.70961975425636 19.979323935774975 3 17.998454675414532 30.726383321263288 32.659385154653606 18.61577684866857 4 11.097082308094052 21.044909518707364 43.28053238858767 24.57747578461091 5 9.916356401952312 41.71223048697426 37.11977429419825 11.25163881687517 6 24.771239894577867 43.66942246654409 18.356534637739294 13.202803001138752 7 23.431926538325897 36.43793796892793 25.363229858476977 14.766905634269193 8 23.339318740426265 35.16323470423385 22.59387980907718 18.903566746262708 9 24.91001553359375 19.604633695245425 25.21449550818004 30.270855262980778 10-11 21.685504723984433 29.454537622945416 30.471752336417147 18.388205316653004 12-13 24.51397273697657 28.56156390200172 29.53947377276686 17.38498958825485 14-15 22.000390359467225 30.043545271602696 29.424892819404903 18.531171549525176 16-17 17.408949935925737 32.81840600467365 30.985559061392838 18.787084998007774 18-19 17.005376098035047 32.45849474639193 34.02703932331698 16.50908983225605 20-21 18.74742569166449 30.25973256806269 33.61972653778748 17.373115202485334 22-23 18.812802106314503 29.78049801523471 33.81571632595647 17.590983552494315 24-25 19.43678314091459 30.006824505223374 33.986439829266224 16.569952524595813 26-27 18.548621580874435 29.823390049536936 35.270433986646566 16.357554382942062 28-29 17.568025974655328 30.249246848069266 35.4231785030651 16.7595486742103 30-31 18.439649486478846 30.294786717098976 33.9691214278984 17.296442368523778 32-33 17.96620618454013 31.332706076183474 33.88251985080433 16.818567888472064 34-35 18.959714829562742 29.754837424312182 34.51553064262964 16.769917103495438 36-37 19.290147139991653 29.216509834013166 34.314176864154646 17.179166161840527 38-39 17.89376761767327 30.896451830891873 34.634965848300226 16.57481470313463 40-41 17.75531356422716 30.605860736832184 34.87999892315354 16.75882677578711 42-43 17.793397907704236 30.89716737827695 32.958100242826916 18.351334471191898 44-45 18.44537741601573 30.42076599089792 33.136045292344804 17.997811300741546 46-47 19.209522450660774 30.88362376466851 32.93835928056663 16.968494504104086 48-49 19.002173869150578 31.116345746148617 32.32698222542283 17.55449815927798 50-51 18.137446914477625 31.1224180749894 32.72585323830099 18.014281772231982 52-53 18.83167405221755 31.77729737296942 31.93115553808629 17.45987303672674 54-55 18.808050785856203 30.36194076060327 32.2937363033798 18.536272150160723 56-57 19.544087063621863 31.01325203087878 31.15162773167498 18.291033173824378 58-59 19.70166049276361 29.988881590282563 32.079438749138525 18.2300191678153 60-61 19.258762702031113 31.615723933325523 31.548150271977253 17.577363092666104 62-63 18.96176658257702 31.009291143125605 32.736808380605716 17.29213389369166 64-65 18.625770868474795 31.219887161413567 31.968240420768655 18.186101549342986 66-67 18.73096125552401 30.71855570048446 31.18389862335491 19.36658442063662 68-69 18.074081253272706 30.807377165033174 32.3113744082209 18.807167173473218 70-71 19.63344442709539 30.501698711563623 32.11655153476302 17.74830532657797 72-73 19.16856573281519 29.57387782352886 32.32751252172204 18.930043921933912 74-75 18.477934574394332 31.94765090010352 31.081268450675026 18.49314607482712 76-77 18.887125498225167 30.312954729319536 31.826643890659014 18.97327588179629 78-79 18.446745701520705 29.948080373789004 32.11343973917691 19.491734185513387 80-81 19.444829586832707 31.282147236868852 30.4775660915556 18.795457084742843 82-83 19.41822068521752 30.31503959701639 31.46182519159087 18.804914526175224 84-85 18.07132414903441 30.51670431385641 31.15100785879477 20.26096367831441 86-87 18.424545420280957 30.755407304505145 30.925554999165417 19.89449227604848 88-89 19.048029326200126 29.84018944100633 30.871937502776593 20.23984373001695 90-91 18.457592891760903 28.403338718362953 32.7228056004308 20.41626278944534 92-93 19.385203092694695 30.518957830806343 31.179197536181945 18.916641540317023 94-95 18.849770155943975 29.07678743294813 31.706095747043662 20.367346664064236 96-97 18.37904122459638 29.12508446559861 31.803889051912353 20.691985257892654 98-99 18.25043311109047 30.16405050068584 30.82499081279505 20.760525575428634 100-101 17.789605746346613 29.616407671846563 31.189087756706403 21.40489882510042 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1044.0 1 702.5 2 381.0 3 400.0 4 345.0 5 343.5 6 567.0 7 975.5 8 1350.0 9 1552.5 10 1450.5 11 1208.0 12 1161.5 13 1118.5 14 1014.5 15 996.5 16 1017.0 17 1025.0 18 959.5 19 910.0 20 973.5 21 1061.5 22 1152.5 23 1289.5 24 1444.5 25 1724.5 26 2393.5 27 3178.0 28 3411.5 29 4197.5 30 6262.0 31 7874.5 32 11151.5 33 15580.0 34 18388.5 35 20774.5 36 24080.5 37 25929.0 38 24278.5 39 21943.5 40 19372.5 41 16394.0 42 13121.5 43 12196.5 44 12741.0 45 11588.5 46 9556.5 47 10462.5 48 16756.5 49 15033.0 50 6797.0 51 3055.0 52 1490.5 53 1080.0 54 879.0 55 796.5 56 697.5 57 611.0 58 589.5 59 579.5 60 516.0 61 467.0 62 437.5 63 332.0 64 195.5 65 142.5 66 112.0 67 77.0 68 65.5 69 53.0 70 38.5 71 33.5 72 21.0 73 9.5 74 9.5 75 12.0 76 9.0 77 6.0 78 9.0 79 9.0 80 6.5 81 4.5 82 2.5 83 2.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02934374991587228 2 0.004038130722367745 3 0.004307339437192261 4 2.692087148245163E-4 5 5.384174296490326E-4 6 0.0 7 0.0 8 0.0 9 0.0016152522889470978 10-11 9.422305018858071E-4 12-13 9.422305018858071E-4 14-15 0.001749856646359356 16-17 0.004038130722367745 18-19 0.0 20-21 0.0 22-23 0.0012114392167103232 24-25 9.422305018858071E-4 26-27 0.005114965581665809 28-29 0.005384174296490326 30-31 0.0010768348592980652 32-33 1.3460435741225816E-4 34-35 0.008614678874384522 36-37 0.011845183452278717 38-39 0.0075378440150864565 40-41 0.0010768348592980652 42-43 2.692087148245163E-4 44-45 0.0014806479315348396 46-47 0.0 48-49 4.0381307223677444E-4 50-51 0.001749856646359356 52-53 0.003499713292718712 54-55 0.004576548152016777 56-57 0.001749856646359356 58-59 8.076261444735489E-4 60-61 0.0033651089353064537 62-63 0.00807626144473549 64-65 0.011710579094866459 66-67 0.0033651089353064537 68-69 0.004172735079780002 70-71 2.692087148245163E-4 72-73 0.0012114392167103232 74-75 0.007941657087323232 76-77 0.004172735079780002 78-79 0.005249569939078068 80-81 0.006326404798376133 82-83 0.007941657087323232 84-85 0.0075378440150864565 86-87 0.004576548152016777 88-89 0.013595040098638073 90-91 0.015883314174646463 92-93 8.076261444735489E-4 94-95 0.0030959002204819373 96-97 0.0016152522889470978 98-99 0.0049803612242535515 100-101 0.006191800440963875 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 371459.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.307000234211582 #Duplication Level Percentage of deduplicated Percentage of total 1 70.44983309559872 12.897251109812927 2 9.951031572136523 3.6434707464350033 3 4.160110583356617 2.28477436271567 4 2.4748908136405747 1.8123130681986437 5 1.574930517771275 1.4416126678852847 6 1.174948164051586 1.290586578868731 7 0.9087834360248813 1.1645969003308574 8 0.7264385394761995 1.0639128409864884 9 0.6337955678425952 1.0442606048042988 >10 6.058556240165875 24.06268255715974 >50 1.1073040895254622 14.298482470474536 >100 0.7279090628354632 24.175481008671213 >500 0.03235151390379836 4.0319389219267805 >1k 0.019116803670426306 6.788636161729827 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4114 1.10752465278806 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3732 1.0046869237250948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3367 0.9064257428141465 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.6057196083551617 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1978 0.5324948379228932 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1579 0.4250805607079112 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1543 0.4153890469742286 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1216 0.3273577972266118 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1144 0.30797476975924665 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1122 0.3020521780331073 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1093 0.2942451253031963 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1060 0.2853612377139873 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1019 0.2743236804061821 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 932 0.2509025222164492 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 847 0.22801978145636528 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 845 0.22748136402671626 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 820 0.2207511461561034 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 810 0.21805905900785819 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 798 0.21482855442996399 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 784 0.2110596324224208 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 773 0.2080983365593511 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 763 0.20540624941110594 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 763 0.20540624941110594 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 689 0.18548480451409174 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 618 0.16637098576155107 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 614 0.165294150902253 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 602 0.16206364632435882 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 585 0.15748709817234202 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 583 0.156948680742693 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 550 0.14806479315348395 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 537 0.14456507986076525 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 521 0.14025774042357297 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 518 0.13945011427909942 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 516 0.1389116968494504 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 509 0.1370272358456788 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 492 0.13245068769366203 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 484 0.13029701797506588 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 483 0.13002780926024138 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 461 0.12410521753410202 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 448 0.1206055042413833 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.11764420837831362 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 435 0.11710579094866458 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 417 0.11226003408182329 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 415 0.11172161665217427 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 415 0.11172161665217427 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 414 0.11145240793734976 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 412 0.11091399050770072 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 412 0.11091399050770072 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 407 0.10956794693357814 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 406 0.1092987382187536 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 397 0.10687585978533297 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 389 0.10472219006673684 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 388 0.10445298135191232 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 387 0.10418377263708781 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 382 0.10283772906296523 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 377 0.10149168548884263 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 372 0.10014564191472006 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.3460435741225816E-4 0.0 20-21 0.0 0.0 0.0 2.692087148245163E-4 0.0 22-23 0.0 0.0 0.0 6.730217870612907E-4 0.0 24-25 0.0 0.0 0.0 0.0016152522889470978 0.0 26-27 0.0 0.0 0.0 0.0032305045778941955 0.0 28-29 0.0 0.0 0.0 0.00659561351320065 0.0 30-31 0.0 0.0 0.0 0.011575974737454201 0.0 32-33 0.0 0.0 0.0 0.01965223618218969 0.0 34-35 0.0 0.0 0.0 0.029478354273284536 0.0 36-37 0.0 0.0 0.0 0.04186195515521228 0.0 38-39 0.0 0.0 0.0 0.055591599611262615 0.0 40-41 0.0 0.0 0.0 0.07160951814332134 0.0 42-43 0.0 0.0 0.0 0.10822190335945556 0.0 44-45 0.0 0.0 0.0 0.14294982757181815 0.0 46-47 0.0 0.0 0.0 0.17404343413404977 0.0 48-49 0.0 0.0 0.0 0.21186725856689434 0.0 50-51 0.0 0.0 0.0 0.26974713225416536 0.0 52-53 0.0 0.0 0.0 0.37783443125620864 0.0 54-55 0.0 0.0 0.0 0.45711639777202867 0.0 56-57 0.0 0.0 0.0 0.6019506863476185 0.0 58-59 0.0 0.0 0.0 0.7811090860633341 0.0 60-61 0.0 0.0 0.0 0.942768919315456 0.0 62-63 0.0 0.0 0.0 1.1615010001103756 0.0 64-65 0.0 0.0 0.0 1.2913942050132046 0.0 66-67 0.0 0.0 0.0 1.5786399037309637 0.0 68-69 0.0 0.0 0.0 1.9085551837484083 0.0 70-71 0.0 0.0 0.0 2.127960286330389 0.0 72-73 0.0 0.0 0.0 2.51965896640006 0.0 74-75 0.0 0.0 0.0 2.9182224686977563 0.0 76-77 0.0 0.0 0.0 3.1369545494926765 0.0 78-79 0.0 0.0 0.0 3.3762810969716712 0.0 80-81 0.0 0.0 0.0 3.838108647253129 0.0 82-83 0.0 0.0 0.0 4.209212860638724 0.0 84-85 0.0 0.0 0.0 4.787473180081785 0.0 86-87 0.0 0.0 0.0 5.5948301158405105 0.0 88-89 0.0 0.0 0.0 6.684856202164976 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTAGA 50 0.0016128235 38.007595 1 TTCGATA 75 6.91294E-6 37.997364 4 GGTACTC 100 3.0231604E-8 37.99736 3 CATATAG 100 3.0231604E-8 37.99736 3 GGATATG 105 4.640424E-8 36.192837 9 GTCTTCG 80 1.0741744E-5 35.632122 1 CTTCGAT 80 1.0761591E-5 35.622524 3 CTCCCGC 95 8.674433E-7 35.00228 7 GTTCGCA 95 8.674433E-7 35.00228 9 GTAAGCG 35 2.053473E-4 33.926216 94-95 TCTTCGA 85 1.6300166E-5 33.527084 2 GTGGTAC 100 1.2940327E-6 33.256645 1 TGGTACT 100 1.2967594E-6 33.247692 2 ACCCTTG 145 6.6029315E-10 32.760757 7 GACATAT 120 1.498538E-7 31.672997 1 TGTTCGC 90 2.407526E-5 31.668734 8 TACTCCC 105 1.8961291E-6 31.668732 5 TGCCCTG 30 0.003951403 31.66447 92-93 CGGTATA 30 0.003951403 31.66447 34-35 CCCTTGG 155 1.3496901E-9 30.64716 8 >>END_MODULE