##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936588_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 389877 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86083559686773 31.0 31.0 34.0 30.0 34.0 2 32.02728809342434 31.0 31.0 34.0 30.0 34.0 3 32.075418657679215 33.0 31.0 34.0 30.0 34.0 4 35.81079930337004 37.0 35.0 37.0 35.0 37.0 5 35.64921500883612 37.0 35.0 37.0 33.0 37.0 6 35.75477650643665 37.0 35.0 37.0 33.0 37.0 7 35.702306111927605 37.0 35.0 37.0 33.0 37.0 8 35.682887166978304 37.0 35.0 37.0 33.0 37.0 9 37.29198183016695 39.0 37.0 39.0 34.0 39.0 10-11 37.18919300189547 39.0 37.0 39.0 33.0 39.0 12-13 37.13019747253621 39.0 37.0 39.0 33.0 39.0 14-15 38.1630642484681 40.0 37.0 41.0 33.0 41.0 16-17 38.07917625302339 40.0 37.0 41.0 33.0 41.0 18-19 38.30202474113631 40.0 37.5 41.0 33.5 41.0 20-21 38.36554349192181 40.0 38.0 41.0 34.0 41.0 22-23 38.259785778591706 40.0 37.5 41.0 34.0 41.0 24-25 38.11712155372079 40.0 37.0 41.0 33.0 41.0 26-27 37.88951387232383 40.0 37.0 41.0 33.0 41.0 28-29 37.82293389966579 40.0 37.0 41.0 33.0 41.0 30-31 37.76513233660872 39.5 37.0 41.0 33.0 41.0 32-33 37.5224134791229 39.0 36.0 41.0 33.0 41.0 34-35 37.340014927784914 39.0 36.0 41.0 32.5 41.0 36-37 37.12266176255588 39.0 35.0 41.0 31.5 41.0 38-39 36.94999063807303 39.0 35.0 41.0 31.0 41.0 40-41 36.82399577302586 39.0 35.0 41.0 31.0 41.0 42-43 36.5278934125378 39.0 35.0 40.5 30.5 41.0 44-45 36.2105599971273 39.0 35.0 40.0 30.0 41.0 46-47 35.79307320000923 39.0 35.0 40.0 29.5 41.0 48-49 35.36441493086281 38.5 35.0 40.0 27.0 41.0 50-51 35.08698512607822 38.0 35.0 40.0 26.5 41.0 52-53 34.81630488589991 38.0 34.0 40.0 24.5 41.0 54-55 34.68800288296052 38.0 34.0 40.0 24.5 41.0 56-57 34.41039866419409 38.0 34.0 40.0 23.5 41.0 58-59 34.115231213946956 38.0 33.5 40.0 23.0 41.0 60-61 33.70874403978691 38.0 33.0 40.0 22.0 41.0 62-63 33.424235079268584 37.0 33.0 40.0 22.0 41.0 64-65 33.02366643838954 37.0 32.5 39.0 20.0 40.0 66-67 32.591287765115666 36.0 32.0 39.0 18.5 40.0 68-69 32.21073954093214 36.0 31.0 39.0 18.0 40.0 70-71 31.829364132790598 35.5 31.0 38.0 13.5 40.0 72-73 31.31342833765521 35.0 31.0 37.5 8.0 39.5 74-75 30.771969108205923 35.0 30.0 37.0 7.0 39.0 76-77 29.182188228595173 33.0 28.5 35.5 2.0 37.5 78-79 29.923842647809437 34.0 29.0 36.0 2.0 38.0 80-81 29.628473852009737 34.0 29.0 36.0 2.0 37.5 82-83 29.274038991784586 34.0 29.0 35.0 2.0 37.0 84-85 28.850831159570838 34.0 29.0 35.0 2.0 36.5 86-87 28.51359787830521 34.0 29.0 35.0 2.0 36.0 88-89 28.025554213251873 33.5 28.0 35.0 2.0 36.0 90-91 27.542689104512448 33.0 26.5 35.0 2.0 35.0 92-93 26.92418891086163 33.0 25.5 35.0 2.0 35.0 94-95 26.420087617376762 32.5 25.0 34.0 2.0 35.0 96-97 25.762954726747154 32.0 24.5 34.0 2.0 35.0 98-99 25.046586487533247 32.0 19.0 34.0 2.0 35.0 100-101 21.966000815641856 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2668017483407219 1101 2 0.2712410367168765 1101 3 0.24728481600562446 1101 4 0.1732951846737052 1101 5 0.32175754481682617 1101 6 0.3536026891582935 1101 7 0.15741933012132137 1101 8 0.20666419291431737 1101 9 0.08056709473126489 1101 10-11 0.2092279223880027 1101 12-13 0.20323487898784975 1101 14-15 0.27532790628414716 1101 16-17 0.42703086411480484 1101 18-19 0.3337962255638445 1101 20-21 0.25178113999218965 1101 22-23 0.19457147064961333 1101 24-25 0.4995873125533805 1101 26-27 0.3596360020087701 1101 28-29 0.3807356851963135 1101 30-31 0.6486052295570985 1101 32-33 0.44828830829451505 1101 34-35 0.3656980098587539 1101 36-37 0.1397685505579105 1101 38-39 0.2526817877175631 1101 40-41 0.12766658363290162 1101 42-43 0.2781699540296785 1101 44-45 0.4389900563466753 1101 46-47 0.5985151649630822 1101 48-49 0.5747439810732828 1101 50-51 0.6742243763766211 1101 52-53 0.7754873967131886 1101 54-55 0.7091442418952525 1101 56-57 0.5526338534614084 1101 58-59 0.5192463249642927 1101 60-61 0.7584026603344327 1101 62-63 0.5712632335206251 1101 64-65 0.7104903126288846 1101 66-67 0.8078031303032489 1101 68-69 0.5246778053592642 1101 70-71 0.6602870017091504 1101 72-73 0.7595644390009149 1101 74-75 0.9786153007949174 1101 76-77 0.8754028031368044 1101 78-79 0.9953713273569598 1101 80-81 0.8215265205195017 1101 82-83 0.34474672525797345 1101 84-85 0.3703930987924551 1101 86-87 0.47434484747408234 1101 88-89 0.5765792009802091 1101 90-91 0.689851049072356 1101 92-93 0.548992973092794 1101 94-95 0.6555455835432831 1101 96-97 0.9101530924557153 1101 98-99 0.587279318748184 1101 100-101 1.0325411162060547 1102 1 0.2473709217135216 1102 2 0.155041486875664 1102 3 0.1380072283634064 1102 4 0.2839324985207128 1102 5 0.21137305987864607 1102 6 0.20435963424508685 1102 7 0.17876795652036037 1102 8 0.20868065762618215 1102 9 0.33260976731783387 1102 10-11 0.12177841108778154 1102 12-13 0.2948514423646529 1102 14-15 0.23698270232521423 1102 16-17 0.3648796600942106 1102 18-19 0.40210165521882857 1102 20-21 0.12277056434393074 1102 22-23 0.33860994997622385 1102 24-25 0.40300666938130547 1102 26-27 0.3777178939089936 1102 28-29 0.3260938080958198 1102 30-31 0.47082796565921825 1102 32-33 0.48708367104232764 1102 34-35 0.5093695789396335 1102 36-37 0.38421864768120884 1102 38-39 0.2454129565887726 1102 40-41 0.4454201062795846 1102 42-43 0.3041419578529414 1102 44-45 0.3617415748617603 1102 46-47 0.33228478232810943 1102 48-49 0.31543864391668563 1102 50-51 0.17927016307535126 1102 52-53 0.2843734078746607 1102 54-55 0.2069520302589538 1102 56-57 0.10650953563696675 1102 58-59 -0.02803268098644196 1102 60-61 0.061468981673812095 1102 62-63 0.09093763921853082 1102 64-65 0.18500084202795364 1102 66-67 -0.042495947796219014 1102 68-69 -0.22010220265726232 1102 70-71 -0.2033426347722127 1102 72-73 0.03349531187730648 1102 74-75 -0.016200164934431882 1102 76-77 -0.11908121005963324 1102 78-79 0.014261038453682318 1102 80-81 0.05612399839697346 1102 82-83 0.2966491086329306 1102 84-85 0.14586561135681464 1102 86-87 0.058025049737210566 1102 88-89 0.30745978556997855 1102 90-91 0.17043101392296833 1102 92-93 0.29757928949891976 1102 94-95 0.32062281969164985 1102 96-97 0.7645390573679975 1102 98-99 0.5988323665698481 1102 100-101 1.0137069041381004 1103 1 0.12292934491688712 1103 2 0.23539605104440042 1103 3 0.19373586694761258 1103 4 0.22955882352762558 1103 5 0.258993424274756 1103 6 0.14845893679216715 1103 7 0.18778261224900206 1103 8 0.11787044470333541 1103 9 0.18622146732090528 1103 10-11 0.15897846024746087 1103 12-13 0.21364272881194069 1103 14-15 0.09456865494757238 1103 16-17 0.2950652378740344 1103 18-19 0.1523044388855297 1103 20-21 0.029238134319655273 1103 22-23 0.22207386370099158 1103 24-25 0.10711457487183651 1103 26-27 0.12428286149432921 1103 28-29 0.07637186751758662 1103 30-31 -0.009303843724133287 1103 32-33 0.3006993652691463 1103 34-35 0.20945560837399313 1103 36-37 -0.0035359840820845534 1103 38-39 0.1252098656741012 1103 40-41 0.32072965861927827 1103 42-43 0.5962226411724529 1103 44-45 0.5763573612629713 1103 46-47 0.6274072206480739 1103 48-49 0.705027546119652 1103 50-51 0.680795649295284 1103 52-53 0.29147698116867105 1103 54-55 0.24504770727988756 1103 56-57 0.18208792995902456 1103 58-59 0.30313762656879106 1103 60-61 0.22249303919063124 1103 62-63 0.4678110837756293 1103 64-65 0.5834185149317435 1103 66-67 0.40878220379993024 1103 68-69 0.8065530958341505 1103 70-71 0.9509388658269202 1103 72-73 0.5551532219765498 1103 74-75 0.4740800452231859 1103 76-77 0.5055047732875266 1103 78-79 0.6224015736796638 1103 80-81 0.6532973169823997 1103 82-83 0.4597532349732987 1103 84-85 1.1147690433167483 1103 86-87 1.1256503301009921 1103 88-89 0.9490903505990147 1103 90-91 1.0748968633100127 1103 92-93 1.3132458639911953 1103 94-95 0.9019344613176052 1103 96-97 1.2043737579548406 1103 98-99 1.0437950359402954 1103 100-101 0.7865323565607483 1104 1 0.12648236939747903 1104 2 0.11303719557186653 1104 3 -0.004968917036059395 1104 4 0.1520317033673635 1104 5 0.1364140796943758 1104 6 0.06203980589723557 1104 7 0.27397810419169843 1104 8 -0.02164876304780705 1104 9 0.12078974964775568 1104 10-11 0.09142374745027126 1104 12-13 0.11802132750917593 1104 14-15 0.1244969014922006 1104 16-17 0.2940100400008063 1104 18-19 0.023286812356275277 1104 20-21 0.05568107803824063 1104 22-23 0.08607303214207462 1104 24-25 0.2552753807280226 1104 26-27 0.10156618369821757 1104 28-29 0.29062113360383535 1104 30-31 0.33624826100189154 1104 32-33 0.32333572203311434 1104 34-35 0.18385209818739412 1104 36-37 0.20474117702615757 1104 38-39 0.4376854543800164 1104 40-41 0.34249823325168904 1104 42-43 0.37088119803974706 1104 44-45 0.3938664013163944 1104 46-47 0.6835279982816544 1104 48-49 0.7221406507322854 1104 50-51 0.6852148809852352 1104 52-53 0.8387151976007772 1104 54-55 0.8435764149617242 1104 56-57 0.7833554273446524 1104 58-59 0.7878388323292285 1104 60-61 0.7786878381675635 1104 62-63 0.5896313158561597 1104 64-65 0.6644057391628735 1104 66-67 0.8288753761972174 1104 68-69 0.6466559697521781 1104 70-71 0.6476891713618258 1104 72-73 0.7526799641554867 1104 74-75 0.7359581084468623 1104 76-77 0.5995512523914606 1104 78-79 0.7418231992513604 1104 80-81 0.8307579754258718 1104 82-83 0.7006879827967545 1104 84-85 0.8528547568825005 1104 86-87 1.0146860367370856 1104 88-89 1.0858865178683494 1104 90-91 0.7733087117646988 1104 92-93 0.9284451231477959 1104 94-95 1.272052202501193 1104 96-97 1.0747214377794592 1104 98-99 1.1815093828515622 1104 100-101 1.8114287266800666 1105 1 -0.10622539785251561 1105 2 -0.001641316021991912 1105 3 0.06473500671339139 1105 4 -0.056977996895909655 1105 5 0.10710210852766977 1105 6 -0.12394056831611522 1105 7 -0.125918416947556 1105 8 -0.12340297611570605 1105 9 -0.08289818063423837 1105 10-11 0.0332608843943305 1105 12-13 0.05089909574467555 1105 14-15 0.14263209979148428 1105 16-17 0.008672361024615327 1105 18-19 0.06045472968582288 1105 20-21 0.12084108310659047 1105 22-23 0.2505164285013066 1105 24-25 -0.11008771040376786 1105 26-27 -0.12331379490207439 1105 28-29 0.07048217627654907 1105 30-31 0.05431236158052144 1105 32-33 -0.01271891762110755 1105 34-35 0.4806058888925975 1105 36-37 0.22974856837335267 1105 38-39 0.41571027522784476 1105 40-41 0.478094257410838 1105 42-43 0.4315272017846965 1105 44-45 0.7742832029044777 1105 46-47 0.6857489221296618 1105 48-49 0.6973162832204238 1105 50-51 0.6481701527756272 1105 52-53 0.8581262832136929 1105 54-55 0.6807186385605348 1105 56-57 0.30873942542994115 1105 58-59 0.5839115631856373 1105 60-61 0.5355600312154749 1105 62-63 0.4970247748658565 1105 64-65 0.48873277459910014 1105 66-67 0.3271524883626711 1105 68-69 0.8102493611838533 1105 70-71 0.8688893149651449 1105 72-73 1.180983896352192 1105 74-75 1.1060111853591117 1105 76-77 0.5899653790157942 1105 78-79 0.7116260262656802 1105 80-81 0.7759440965876543 1105 82-83 0.8899663057352747 1105 84-85 0.750782011947333 1105 86-87 0.9098292176913425 1105 88-89 0.7792951960138979 1105 90-91 0.6488067477781563 1105 92-93 0.782332267835173 1105 94-95 1.0000594249033092 1105 96-97 1.0745366540995533 1105 98-99 1.1940429958828567 1105 100-101 1.8146527619332709 1106 1 -0.08476942916367136 1106 2 -0.17450237277346048 1106 3 -0.1949401400576427 1106 4 0.0205640774680802 1106 5 0.08460113005408942 1106 6 -0.25493861136894935 1106 7 -0.20786362242700562 1106 8 0.008049078678816102 1106 9 -0.10710953092777942 1106 10-11 0.00500648126321579 1106 12-13 -0.013323996231839885 1106 14-15 0.05156164969363175 1106 16-17 0.10184457237490818 1106 18-19 0.2728911289030336 1106 20-21 -0.20924697951571858 1106 22-23 -0.447495313181669 1106 24-25 -0.3083792955309761 1106 26-27 -0.10768561486293748 1106 28-29 -0.2921342620796139 1106 30-31 0.04156480776448035 1106 32-33 -0.13135492544889615 1106 34-35 -0.19650761404282235 1106 36-37 -0.2836760891608918 1106 38-39 0.07144804430807739 1106 40-41 0.11817449224449916 1106 42-43 0.15421213329154426 1106 44-45 0.1753556099494844 1106 46-47 0.3011970630298606 1106 48-49 0.07970180181141728 1106 50-51 0.2733227946542982 1106 52-53 0.001513758751855221 1106 54-55 0.21258360920632668 1106 56-57 0.08377269353170647 1106 58-59 -0.20878706695134497 1106 60-61 -0.03879417622092518 1106 62-63 0.09667448132378809 1106 64-65 0.17649207009812784 1106 66-67 0.19032861360728504 1106 68-69 0.09607323593922601 1106 70-71 -0.14360579266694984 1106 72-73 -0.006838021456026411 1106 74-75 -0.11720016493443453 1106 76-77 -0.10048471883156296 1106 78-79 -0.44749334751123016 1106 80-81 -0.1799110893223279 1106 82-83 0.07310524898380422 1106 84-85 0.3817252604796231 1106 86-87 0.5570601374565065 1106 88-89 0.5115124171489249 1106 90-91 0.2644310139229695 1106 92-93 0.24419332458663945 1106 94-95 0.13557018811270183 1106 96-97 0.5031530924557153 1106 98-99 0.2715165770961647 1106 100-101 0.38604023747143046 1107 1 -0.17090934685914405 1107 2 -0.0715558707158479 1107 3 -0.2940265598107281 1107 4 -0.24697501718212322 1107 5 -0.023324795871836557 1107 6 -0.24168758256236345 1107 7 -0.16769078292083606 1107 8 -0.2180249953952611 1107 9 -0.11724944862325515 1107 10-11 0.009097016242641587 1107 12-13 -0.21627049828945388 1107 14-15 -0.30777579886603235 1107 16-17 -0.06088382268681869 1107 18-19 -0.38540105216691956 1107 20-21 -0.057909530956045785 1107 22-23 -0.30328955186479334 1107 24-25 -0.05446571528405997 1107 26-27 -0.21125763132383923 1107 28-29 -0.3649285007627441 1107 30-31 -0.2533652333877825 1107 32-33 0.08119239965397895 1107 34-35 -0.04912078276710474 1107 36-37 -0.11318226200040016 1107 38-39 -0.3349964001363688 1107 40-41 -0.26928641310529144 1107 42-43 -0.22025700251092672 1107 44-45 0.004063428879526043 1107 46-47 -0.1764654884104715 1107 48-49 -0.4000800911926987 1107 50-51 -0.3972080695432325 1107 52-53 -0.07512410133044511 1107 54-55 -0.19988141136980886 1107 56-57 -0.34213677148887456 1107 58-59 -0.15509570892665892 1107 60-61 -0.20572833259952716 1107 62-63 -0.23901687670090865 1107 64-65 -0.22385772414055793 1107 66-67 0.21860433377189992 1107 68-69 -0.25198437722949407 1107 70-71 -0.20634241818135024 1107 72-73 -0.41156641651775416 1107 74-75 -0.587805103206037 1107 76-77 -0.6564106447574858 1107 78-79 -0.42782256561822507 1107 80-81 -0.13898104817006285 1107 82-83 -0.0883844629503514 1107 84-85 0.007009211096907109 1107 86-87 0.11856219506967491 1107 88-89 -0.04988264457946556 1107 90-91 -0.007141002537935037 1107 92-93 0.12488056738499509 1107 94-95 0.2331092827629071 1107 96-97 0.6457498002746433 1107 98-99 0.567578305491228 1107 100-101 0.7579455872656702 1108 1 -0.0771779802325554 1108 2 0.07075178399613336 1108 3 -0.11457434433317815 1108 4 -0.14585872538227562 1108 5 -0.24585492695303657 1108 6 -0.11161319569198724 1108 7 0.033974857382972345 1108 8 -0.04062550564422196 1108 9 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-0.9248466410505607 2316 84-85 -1.069037626118316 2316 86-87 -1.2884209621998473 2316 88-89 -1.7716594041569174 2316 90-91 -1.1682287798914608 2316 92-93 -0.93721904654738 2316 94-95 -1.1335913239147892 2316 96-97 -2.002218041564902 2316 98-99 -2.631607134244039 2316 100-101 -2.5569116525629347 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 0.0 5 11.0 6 31.0 7 70.0 8 127.0 9 278.0 10 489.0 11 745.0 12 1183.0 13 1527.0 14 2110.0 15 2960.0 16 4154.0 17 5430.0 18 4699.0 19 2751.0 20 2486.0 21 2640.0 22 2878.0 23 3115.0 24 3606.0 25 4241.0 26 4990.0 27 6081.0 28 7328.0 29 8898.0 30 10759.0 31 13470.0 32 16611.0 33 21065.0 34 27604.0 35 37782.0 36 53379.0 37 78106.0 38 55149.0 39 3122.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.86511100507437 24.158931970590196 17.173590694762982 18.80236632957245 2 14.280107431720063 25.824399929199725 39.79642356007378 20.099069079006437 3 17.87220398112046 30.696952595936793 32.77498460907039 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29.7382251325418 33.77860196933905 17.725718624078876 24-25 19.498457205734116 30.00664312077912 33.942884550768575 16.552015122718192 26-27 18.62267439399905 29.79558649452448 35.21839888270879 16.363340228767683 28-29 17.57550340950043 30.170712282281244 35.35415328067428 16.899631027544043 30-31 18.461700250338573 30.36753088193048 33.95114293922108 17.21962592850987 32-33 18.01332810059176 31.34873914013744 33.850836084370265 16.78709667490054 34-35 18.97242934201313 29.63621300378843 34.561560105778575 16.82979754841986 36-37 19.118539123386824 29.154948182176106 34.476262293988725 17.250250400448348 38-39 17.96734329552625 30.975648542862565 34.64375670088285 16.413251460728336 40-41 17.647254964071955 30.544672479618544 35.01442123339057 16.793651322918922 42-43 17.719195870338904 30.91166757510661 33.03164577254801 18.337490782006476 44-45 18.532459537794647 30.34652576499859 33.07717957267807 18.04383512452869 46-47 19.267585764668162 30.6953510740622 33.05662071176659 16.980442449503048 48-49 18.893443043429194 31.004857954836922 32.438775405513546 17.662923596220338 50-51 18.15433861748528 31.041624719622753 32.80923574516545 17.99480091772651 52-53 18.77055151151398 31.62534562448381 31.978536655432265 17.62556620856995 54-55 18.85968512258058 30.451231961750963 32.163871689247074 18.525211226421387 56-57 19.599679333012666 30.858297258297256 31.265287798621134 18.276735610068943 58-59 19.840131568819626 29.8236341524741 32.10992461989235 18.22630965881393 60-61 19.308685239748737 31.573897461091576 31.516944228161837 17.60047307099785 62-63 18.946509721832083 30.959408032199597 32.72561461295449 17.368467633013825 64-65 18.699196377606324 31.1700305928078 31.989045594186727 18.14172743539915 66-67 18.746569334078888 30.778267404698024 31.09029769202752 19.38486556919557 68-69 18.01763905471903 30.833462648782234 32.322535386477924 18.826362910020812 70-71 19.62010949649311 30.496530302622247 32.08049749214813 17.80286270873651 72-73 19.19338559774698 29.507805676925898 32.45861189376636 18.840196831560764 74-75 18.32560847673694 31.96987690673999 31.165243531768528 18.539271084754542 76-77 18.904797570434816 30.33660455953871 31.865086286499906 18.89351158352656 78-79 18.402539519671656 29.95953442139353 32.185205374034055 19.45272068490076 80-81 19.58292394480374 31.224522779104642 30.494036823162112 18.69851645292951 82-83 19.494152793152413 30.127641889525826 31.585178610138748 18.793026707183014 84-85 18.164895391262846 30.397857021896762 31.24252703097979 20.194720555860606 86-87 18.381916289473786 30.77239781803164 31.016221110915872 19.829464781578707 88-89 19.065227265145666 29.73333264904452 30.91988945314204 20.281550632667777 90-91 18.515724045188346 28.30263975075579 32.78811162609262 20.39352457796324 92-93 19.51952337211152 30.493818924508258 31.111413308427714 18.875244394952507 94-95 18.852250800558338 29.012413786025128 31.850033869775846 20.285301543640692 96-97 18.30014282249589 29.075349453155376 31.884448516532377 20.740059207816355 98-99 18.23746588078583 30.152594856869335 30.862248846010214 20.74769041633462 100-101 17.69971470702606 29.477450561409857 31.370291799655803 21.45254293190828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1208.0 1 806.5 2 420.0 3 427.5 4 370.0 5 372.5 6 589.0 7 1050.5 8 1491.0 9 1682.0 10 1531.0 11 1266.0 12 1183.5 13 1133.0 14 1074.5 15 1043.5 16 995.5 17 958.5 18 963.0 19 997.0 20 1041.0 21 1097.0 22 1169.0 23 1283.0 24 1428.5 25 1745.5 26 2503.5 27 3361.5 28 3682.5 29 4430.5 30 6525.5 31 8235.0 32 11488.5 33 16146.0 34 19402.0 35 21883.0 36 25191.5 37 27065.5 38 25456.5 39 23067.5 40 20276.0 41 17256.5 42 13965.5 43 13155.0 44 13363.5 45 11951.5 46 10016.0 47 10926.5 48 17679.5 49 15994.5 50 7172.5 51 3155.5 52 1494.5 53 1089.0 54 947.0 55 832.0 56 730.5 57 648.0 58 662.0 59 655.5 60 576.0 61 507.5 62 445.0 63 348.5 64 224.0 65 153.5 66 110.0 67 84.0 68 72.5 69 60.5 70 40.0 71 26.5 72 17.5 73 11.0 74 13.5 75 12.0 76 7.0 77 6.0 78 6.5 79 9.5 80 7.0 81 2.5 82 1.0 83 0.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019236836232965782 2 0.012311575189098102 3 0.009490172541596453 4 0.0 5 0.002564911497728771 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001154210173977947 14-15 2.564911497728771E-4 16-17 1.2824557488643856E-4 18-19 7.694734493186314E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.001410701323750824 28-29 0.0026931570726152097 30-31 0.0012824557488643855 32-33 0.006155787594549051 34-35 0.0015389468986372627 36-37 3.847367246593157E-4 38-39 7.694734493186314E-4 40-41 1.2824557488643856E-4 42-43 0.003719121671706718 44-45 0.0017954380484101397 46-47 5.129822995457542E-4 48-49 2.564911497728771E-4 50-51 1.2824557488643856E-4 52-53 7.694734493186314E-4 54-55 0.002821402647501648 56-57 0.01654367916035057 58-59 0.030522446822972376 60-61 0.021417011006035238 62-63 0.015004732261713309 64-65 0.020391046406943728 66-67 0.002821402647501648 68-69 8.977190242050699E-4 70-71 3.847367246593157E-4 72-73 1.2824557488643856E-4 74-75 0.002564911497728771 76-77 0.003334384947047402 78-79 0.010131400416028646 80-81 0.017441398184555642 82-83 0.019365081807852222 84-85 0.035652269818429914 86-87 0.011670347314665907 88-89 0.045655424659572126 90-91 0.05617156180026009 92-93 0.035652269818429914 94-95 0.038217181316158685 96-97 0.05963419232219393 98-99 0.06425103301810571 100-101 0.07117629406197339 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 389877.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.675574604298276 #Duplication Level Percentage of deduplicated Percentage of total 1 69.89682643332898 12.354665702259943 2 9.951678202951546 3.5180326102847816 3 4.327195159113665 2.2945698258681584 4 2.4683296330155415 1.7451657830546559 5 1.6223354084135069 1.4337855272303828 6 1.2290859489501256 1.3034880231457613 7 0.9722403610349281 1.2029434924347935 8 0.7850478139102927 1.110093696217012 9 0.6312306821644682 1.0041628513608136 >10 6.154136374849448 23.49561528379463 >50 1.1115464426160522 13.844109808991039 >100 0.7995588640749932 25.790954583112107 >500 0.03192431036234092 4.079235245987837 >1k 0.018864365214110543 6.823177566258076 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4414 1.1321519350974796 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3932 1.0085232009069527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3489 0.8948976215575681 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.6119878833580847 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2092 0.5365794853248589 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1695 0.4347524988650267 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1650 0.42321039712524716 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1292 0.3313865655065572 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1166 0.29906868063517467 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1150 0.29496482223880865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1144 0.2934258753401714 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1108 0.2841921939483478 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1084 0.2780364063537988 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 986 0.25290027367605683 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 952 0.244179574583779 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 891 0.22853361444763348 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 873 0.22391677375172167 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 872 0.22366028260194884 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 864 0.2216083534037658 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 834 0.2139136189105795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.21006625166398635 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 775 0.19878064107397975 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 771 0.19775467647488826 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 703 0.18031327829033258 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 683 0.17518345529487506 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 665 0.17056661459896327 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 665 0.17056661459896327 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 613 0.15722907481077367 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 607 0.15569012791213638 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 602 0.154407672163272 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 572 0.1467129376700857 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 564 0.1446610084719027 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 562 0.14414802617235692 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 521 0.13363188903166898 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 510 0.1308104863841673 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 493 0.12645013683802842 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 492 0.12619364568825553 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 484 0.12414171649007251 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 478 0.12260276959143523 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 477 0.12234627844166238 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 476 0.1220897872918895 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 475 0.12183329614211662 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 467 0.1197813669439336 No Hit CAATTATCTCATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTC 464 0.11901189349461498 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 459 0.11772943774575058 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 444 0.11388207049915743 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 442 0.11336908819961167 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 434 0.11131715900142866 No Hit ACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTAT 430 0.11029119440233714 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 428 0.10977821210279139 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 423 0.108495756353927 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.10644382715574399 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 413 0.10593084485619823 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 411 0.10541786255665248 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 395 0.10131400416028644 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 395 0.10131400416028644 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 393 0.10080102186074069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 2.5649114977287706E-4 0.0 22-23 0.0 0.0 0.0 7.694734493186312E-4 0.0 24-25 0.0 0.0 0.0 0.0017954380484101395 0.0 26-27 0.0 0.0 0.0 0.0033343849470474024 0.0 28-29 0.0 0.0 0.0 0.00705350661875412 0.0 30-31 0.0 0.0 0.0 0.01308104863841673 0.0 32-33 0.0 0.0 0.0 0.020006309682284413 0.0 34-35 0.0 0.0 0.0 0.029368236648994427 0.0 36-37 0.0 0.0 0.0 0.040269110514341705 0.0 38-39 0.0 0.0 0.0 0.05270893127832624 0.0 40-41 0.0 0.0 0.0 0.06296857726924132 0.0 42-43 0.0 0.0 0.0 0.09426049754153233 0.0 44-45 0.0 0.0 0.0 0.1318364509832588 0.0 46-47 0.0 0.0 0.0 0.16659100177748365 0.0 48-49 0.0 0.0 0.0 0.20814256804068976 0.0 50-51 0.0 0.0 0.0 0.2607232537441295 0.0 52-53 0.0 0.0 0.0 0.3575486627833907 0.0 54-55 0.0 0.0 0.0 0.4402670585851435 0.0 56-57 0.0 0.0 0.0 0.578131051588065 0.0 58-59 0.0 0.0 0.0 0.7443373166408893 0.0 60-61 0.0 0.0 0.0 0.9034900750749595 0.0 62-63 0.0 0.0 0.0 1.1107349240914441 0.0 64-65 0.0 0.0 0.0 1.2362873419052676 0.0 66-67 0.0 0.0 0.0 1.5049618212923561 0.0 68-69 0.0 0.0 0.0 1.8258322496582255 0.0 70-71 0.0 0.0 0.0 2.030383941602095 0.0 72-73 0.0 0.0 0.0 2.396909794627536 0.0 74-75 0.0 0.0 0.0 2.7621531919041136 0.0 76-77 0.0 0.0 0.0 2.973373653742078 0.0 78-79 0.0 0.0 0.0 3.2103714761322157 0.0 80-81 0.0 0.0 0.0 3.6597439705342967 0.0 82-83 0.0 0.0 0.0 4.010495617848706 0.0 84-85 0.0 0.0 0.0 4.577725795571424 0.0 86-87 0.0 0.0 0.0 5.379773620911211 0.0 88-89 0.0 0.0 0.0 6.417536812892271 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACCA 35 2.7880503E-4 54.27626 9 GTAAGCG 25 2.820494E-5 47.516106 94-95 AGGGTCT 75 1.3601857E-7 44.3313 5 ATGTATT 65 2.5812042E-6 43.855396 1 GTATGGT 70 4.296626E-6 40.722866 1 TGGGATA 75 6.9187918E-6 37.99338 6 CATGGGT 80 1.07612195E-5 35.623363 4 TGGGGAG 80 1.0770686E-5 35.618793 6 CATATAG 95 8.674724E-7 35.002884 3 ACCCTTG 180 0.0 34.29958 7 ATAAGCC 170 3.6379788E-12 33.532173 3 TAGGGTC 105 1.8980918E-6 31.665213 4 CCCTTGG 195 0.0 31.661152 8 TATAAGC 200 0.0 30.877544 2 GGGATGC 95 3.489136E-5 29.994774 7 CTCGTCC 210 1.8189894E-12 29.399641 9 CCTTGGC 210 1.8189894E-12 29.399641 9 TCTAAGG 65 0.005837045 29.23318 3 AAGCCTC 195 1.6370905E-11 29.229427 5 GGACAAT 265 0.0 28.67425 6 >>END_MODULE