FastQCFastQC Report
Thu 26 May 2016
SRR936587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367603
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113320.4786275401745985No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75480.31880344804428784No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72920.3079908244752182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59170.24991520960228553No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50780.21447852532709244No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC47420.20028695689268852No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35170.14854686364225758No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33640.1420846315873058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32850.13874792353278823No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27940.11800964942179919No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25490.10766163077171298No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23900.10094597785186114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG22400.026.5083051
TACCTGG21950.025.53742
GTACATG105400.024.2472041
TACATGG106850.023.118412
GTGTAGC15000.022.48471
ACATGGG104650.022.2889583
GAGTACT75500.021.55319412-13
CATGGGG49900.020.469774
GTACTTT79000.020.35881614-15
AGAGTAC135300.020.17391810-11
AGTACTT78850.019.55289312-13
TAGCCCT16250.017.2493884
ACTTTTT97450.017.18819816-17
TACTTTT90750.016.78040114-15
GTATCAA239500.016.7003881
GTAGCCC14000.016.6270663
GTACACG3452.0962034E-816.5227431
TACACTG19350.016.204595
GAGTACA86950.016.1711941
AGTACAT81300.015.9514912
Produced by FastQC (version 0.11.5)