##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936587_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2367603 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.379774396298703 31.0 31.0 34.0 30.0 34.0 2 31.460031094740124 31.0 31.0 34.0 28.0 34.0 3 31.47304045483977 31.0 31.0 34.0 28.0 34.0 4 35.101684699673044 37.0 35.0 37.0 32.0 37.0 5 35.10921890198652 37.0 35.0 37.0 32.0 37.0 6 35.10616011214718 37.0 35.0 37.0 32.0 37.0 7 35.00997253340193 37.0 35.0 37.0 32.0 37.0 8 34.96877136918647 37.0 35.0 37.0 32.0 37.0 9 36.46217038920799 39.0 37.0 39.0 32.0 39.0 10-11 36.3348107769757 39.0 37.0 39.0 32.0 39.0 12-13 36.23396088786845 39.0 35.0 39.0 32.0 39.0 14-15 37.319622208621965 40.0 37.0 41.0 32.0 41.0 16-17 37.21544595947885 39.0 37.0 41.0 31.0 41.0 18-19 37.18187276329689 39.0 36.5 41.0 31.0 41.0 20-21 37.03744525581358 39.0 36.0 41.0 31.0 41.0 22-23 37.15239020224252 39.0 36.5 41.0 31.0 41.0 24-25 37.188783339098656 39.0 37.0 41.0 31.0 41.0 26-27 37.09758435007896 39.0 36.0 41.0 31.0 41.0 28-29 36.960336889250435 39.0 36.0 41.0 30.5 41.0 30-31 36.76019395988263 39.0 36.0 41.0 30.0 41.0 32-33 36.57333957593397 39.0 35.5 41.0 30.0 41.0 34-35 36.37324944257969 39.0 35.0 40.5 30.0 41.0 36-37 36.163783159592214 39.0 35.0 40.0 30.0 41.0 38-39 35.97123567591357 38.0 35.0 40.0 29.0 41.0 40-41 35.76771591352097 38.0 35.0 40.0 28.0 41.0 42-43 35.52958181755979 38.0 35.0 40.0 27.0 41.0 44-45 35.264402013344295 38.0 34.0 40.0 26.5 41.0 46-47 35.08273895581311 38.0 34.0 40.0 25.5 41.0 48-49 34.920660685089516 38.0 34.0 40.0 25.0 41.0 50-51 33.95471875985965 37.5 33.0 39.5 23.5 40.5 52-53 34.07255185941224 37.5 33.0 39.5 24.0 40.5 54-55 34.510075591220314 38.0 34.0 40.0 24.0 41.0 56-57 34.39099692811675 38.0 34.0 40.0 23.5 41.0 58-59 34.14968071082863 38.0 33.5 40.0 23.0 41.0 60-61 33.839183342815495 37.5 33.0 40.0 22.5 41.0 62-63 33.47771670334933 37.0 33.0 40.0 22.0 41.0 64-65 33.12076834672029 36.5 32.0 39.5 20.5 41.0 66-67 32.642043873064864 36.0 32.0 39.0 19.0 41.0 68-69 32.23498724237129 35.0 31.0 39.0 18.0 40.0 70-71 31.81355108943518 35.0 31.0 38.0 16.0 40.0 72-73 31.413978821618322 35.0 31.0 37.5 13.5 39.5 74-75 30.980014808225874 35.0 30.5 37.0 9.5 39.0 76-77 30.51698194334101 34.5 30.0 36.0 7.5 39.0 78-79 30.012287321818732 34.0 29.5 36.0 6.5 38.5 80-81 29.523600873964092 34.0 29.0 35.5 2.0 37.0 82-83 29.09827872324879 34.0 29.0 35.0 2.0 37.0 84-85 28.718619844627668 34.0 29.0 35.0 2.0 36.0 86-87 28.36385491993379 34.0 27.5 35.0 2.0 36.0 88-89 27.94211022709466 34.0 27.0 35.0 2.0 36.0 90-91 27.505120368575305 33.0 26.0 35.0 2.0 35.0 92-93 27.02471022380019 33.0 25.5 35.0 2.0 35.0 94-95 26.50029586885977 33.0 25.0 35.0 2.0 35.0 96-97 25.88054331743962 32.5 23.5 35.0 2.0 35.0 98-99 25.102270313055016 32.0 19.0 35.0 2.0 35.0 100-101 22.222873091476906 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.415134278778055 1101 2 0.3972123894306101 1101 3 0.4968552048725918 1101 4 0.4398294311109652 1101 5 0.3451271690218505 1101 6 0.42364389308895767 1101 7 0.4063620571843387 1101 8 0.4870459587258722 1101 9 0.395542621256908 1101 10-11 0.7139921597095977 1101 12-13 0.7225961521557096 1101 14-15 0.7912661675013126 1101 16-17 0.7473710803985867 1101 18-19 0.6598993980587409 1101 20-21 0.8653596840904711 1101 22-23 0.7479060777837603 1101 24-25 0.7518960901440721 1101 26-27 0.707135701366667 1101 28-29 0.6874124245453217 1101 30-31 0.6693473326679751 1101 32-33 0.5522968289991539 1101 34-35 0.41813927771220705 1101 36-37 0.33390598153239637 1101 38-39 0.2662032845108584 1101 40-41 0.36913239761312155 1101 42-43 0.5073204712679669 1101 44-45 0.44522555270607 1101 46-47 0.46047613559652234 1101 48-49 0.5478985900314086 1101 50-51 0.28025516409566364 1101 52-53 0.46524633243976155 1101 54-55 0.3583270003889609 1101 56-57 0.3886882880562652 1101 58-59 0.4061970713334162 1101 60-61 0.4132793229011824 1101 62-63 0.49343259726354205 1101 64-65 0.41365701134519384 1101 66-67 0.458621934652804 1101 68-69 0.4146570031013326 1101 70-71 0.41669454728106814 1101 72-73 0.3116189563687577 1101 74-75 0.2739803936021161 1101 76-77 0.3041617801565586 1101 78-79 0.25225945447476406 1101 80-81 0.3033917812338309 1101 82-83 0.22819114021676867 1101 84-85 0.26596108655184736 1101 86-87 0.2651809182780944 1101 88-89 0.23066668891993913 1101 90-91 0.3384607904676038 1101 92-93 0.37190213145364126 1101 94-95 0.5162153068768269 1101 96-97 0.2742546675276891 1101 98-99 0.2781355212781804 1101 100-101 0.5587661732830149 1102 1 0.32538573929054593 1102 2 0.3337229416568803 1102 3 0.42664702298935 1102 4 0.304246600937212 1102 5 0.3450273532102699 1102 6 0.43029482777927797 1102 7 0.403349691323605 1102 8 0.4139920845540601 1102 9 0.47840763637994854 1102 10-11 0.5637020346950052 1102 12-13 0.48195765112553346 1102 14-15 0.4798846225032207 1102 16-17 0.49692897168598904 1102 18-19 0.561131652502425 1102 20-21 0.4916445772498861 1102 22-23 0.2653465367105028 1102 24-25 0.42089414656071966 1102 26-27 0.17959783727436474 1102 28-29 0.16319249452939033 1102 30-31 0.561724700468524 1102 32-33 0.4506080584662939 1102 34-35 0.47297676627106 1102 36-37 0.13048794914687534 1102 38-39 -0.023605803624626276 1102 40-41 0.12812429558253058 1102 42-43 0.2116829872290822 1102 44-45 0.28244338470663877 1102 46-47 0.2879633384561515 1102 48-49 0.4783373276062193 1102 50-51 0.5295112538018287 1102 52-53 0.6883426754144253 1102 54-55 0.5067597038154688 1102 56-57 0.4431740671280906 1102 58-59 0.4820081218854213 1102 60-61 0.46510350394070343 1102 62-63 0.40550946297172175 1102 64-65 0.4739170409509441 1102 66-67 0.4286343457131494 1102 68-69 0.46937395935940174 1102 70-71 0.3674514524635697 1102 72-73 0.11683405002611735 1102 74-75 0.25555072231629694 1102 76-77 0.3171952768362729 1102 78-79 0.31659969079340655 1102 80-81 0.4333684469337271 1102 82-83 0.43842631003547794 1102 84-85 0.10785472432968035 1102 86-87 0.31465895691622947 1102 88-89 0.3924186824111935 1102 90-91 0.41161965957329016 1102 92-93 0.37104757627433216 1102 94-95 0.024585239341067933 1102 96-97 0.2143265725783685 1102 98-99 0.33096774784877425 1102 100-101 0.7308293284419882 1103 1 0.27570619467366697 1103 2 0.20512687913103633 1103 3 0.30154117187686325 1103 4 0.4461662626844003 1103 5 0.47385945769889304 1103 6 0.4432398237164179 1103 7 0.46766517498094373 1103 8 0.5203344050458156 1103 9 0.17543219704767665 1103 10-11 0.423318919197861 1103 12-13 0.20765783316537778 1103 14-15 0.4714669515326122 1103 16-17 0.5231526319439013 1103 18-19 0.6966640722307673 1103 20-21 0.7163020957413977 1103 22-23 0.37284947486455877 1103 24-25 0.5015084949164006 1103 26-27 0.30277576717698906 1103 28-29 0.4936482333848673 1103 30-31 0.4146323094985078 1103 32-33 0.3694610443101922 1103 34-35 0.7158183423947904 1103 36-37 0.7442092222381973 1103 38-39 0.5656817829486158 1103 40-41 0.5074014695624456 1103 42-43 0.5743992431247804 1103 44-45 0.7690497753054046 1103 46-47 0.814705020551358 1103 48-49 0.6793437757644512 1103 50-51 0.7077794386618734 1103 52-53 0.8373064846315401 1103 54-55 0.8686659278876263 1103 56-57 0.83787153488597 1103 58-59 0.8598489838651986 1103 60-61 0.9538040481344439 1103 62-63 1.0349017826343285 1103 64-65 1.0200661868401255 1103 66-67 1.1240908655799942 1103 68-69 0.9202113667012313 1103 70-71 0.9018710513553927 1103 72-73 0.7280673873358374 1103 74-75 0.9043301780797641 1103 76-77 0.8762485626741281 1103 78-79 0.8644585695285549 1103 80-81 0.9406527821459463 1103 82-83 0.6752056866279297 1103 84-85 0.8656474524719293 1103 86-87 0.8931744040907077 1103 88-89 0.7122769395468858 1103 90-91 0.7762749366691715 1103 92-93 0.7371251900813292 1103 94-95 0.7003998582690407 1103 96-97 1.0604330971794234 1103 98-99 1.1105385901267368 1103 100-101 0.9809492885783122 1104 1 0.4572048058037801 1104 2 0.5223498235351904 1104 3 0.39046537926319047 1104 4 0.46663314190060845 1104 5 0.48761405875799113 1104 6 0.5137845617468244 1104 7 0.5518367106742161 1104 8 0.586679008978443 1104 9 0.5127858183719667 1104 10-11 0.7561391460694225 1104 12-13 0.693957623808636 1104 14-15 0.5288327511248454 1104 16-17 0.44570418433098524 1104 18-19 0.49836704564383183 1104 20-21 0.31354282862682936 1104 22-23 0.46050748447034096 1104 24-25 0.6662983171684189 1104 26-27 0.38676572352679983 1104 28-29 0.49357865305618986 1104 30-31 0.6673996498468924 1104 32-33 0.5141964064426219 1104 34-35 0.5643395722630231 1104 36-37 0.36981984606655516 1104 38-39 0.3397503507022037 1104 40-41 0.1953754983797822 1104 42-43 0.49510955086551434 1104 44-45 0.4739825895799754 1104 46-47 0.39817740051331896 1104 48-49 0.5691617175003358 1104 50-51 0.40826102758375526 1104 52-53 0.6633023659139496 1104 54-55 0.5481147586370767 1104 56-57 0.4455244131990739 1104 58-59 0.39705085576076016 1104 60-61 0.415200922835119 1104 62-63 0.5614338497926852 1104 64-65 0.6838181196270341 1104 66-67 0.5960517035542949 1104 68-69 0.6159267373173947 1104 70-71 0.518152711431199 1104 72-73 0.39127499259575416 1104 74-75 0.6161009529733725 1104 76-77 0.4792649417603485 1104 78-79 0.6481905928998799 1104 80-81 0.7206843224915929 1104 82-83 0.575819799735303 1104 84-85 0.41794545058361265 1104 86-87 0.5076503964827133 1104 88-89 0.44224081524644987 1104 90-91 0.4015463478430732 1104 92-93 0.06736088745821078 1104 94-95 -0.007812262528428704 1104 96-97 0.22340377355221364 1104 98-99 0.21701092903835573 1104 100-101 0.5002363809826313 1105 1 0.25107987840529233 1105 2 0.20275463195520516 1105 3 0.256423807198491 1105 4 0.3111324994872504 1105 5 0.24080764518458864 1105 6 0.3322902906659806 1105 7 0.4553430556061926 1105 8 0.42380240514508216 1105 9 0.49198429770033414 1105 10-11 0.44756741577742787 1105 12-13 0.2921465461382198 1105 14-15 0.15952602954503448 1105 16-17 0.2886599898117339 1105 18-19 0.19341148656820195 1105 20-21 0.2519591476862644 1105 22-23 0.01557163780473303 1105 24-25 0.016717029877646894 1105 26-27 -0.20109424796060438 1105 28-29 -0.08577048540588805 1105 30-31 -0.2526802558151431 1105 32-33 -0.3577443859258622 1105 34-35 -0.1994645708631566 1105 36-37 -0.05333167548509721 1105 38-39 -0.04348811665791885 1105 40-41 -0.1844414578129019 1105 42-43 -0.01734552420004576 1105 44-45 0.11897898150493091 1105 46-47 0.20644006837680706 1105 48-49 0.14916734961747835 1105 50-51 0.21454717433560688 1105 52-53 0.09790714969345515 1105 54-55 0.06270546212370931 1105 56-57 0.015694256731812573 1105 58-59 -0.04387351546616003 1105 60-61 0.09562333162121917 1105 62-63 0.07616746825194554 1105 64-65 0.06762824687767477 1105 66-67 0.2762013066660387 1105 68-69 0.40243605671123817 1105 70-71 0.12028129970583734 1105 72-73 -0.21021399837525934 1105 74-75 -0.017581571623136227 1105 76-77 -0.107900519281241 1105 78-79 0.07385298267758955 1105 80-81 0.22401747982126352 1105 82-83 -0.0397082992491562 1105 84-85 -0.03521434314009397 1105 86-87 -0.03589889334251595 1105 88-89 -0.05007275287576718 1105 90-91 -0.20438532753577832 1105 92-93 -0.21986230278414354 1105 94-95 -0.10191613988907733 1105 96-97 -0.03952140729154863 1105 98-99 0.15255557236693562 1105 100-101 0.4426842398980959 1106 1 0.33133832431103016 1106 2 0.3569416474863374 1106 3 0.43664799272098875 1106 4 0.4296613081419238 1106 5 0.3598211481032152 1106 6 0.45772354025972817 1106 7 0.48611089931537066 1106 8 0.6738488189484784 1106 9 0.6517633290475047 1106 10-11 0.7332263078615497 1106 12-13 0.7350963002207962 1106 14-15 0.7025095391209888 1106 16-17 0.6337365563587483 1106 18-19 0.5377763026692861 1106 20-21 0.34842794801747345 1106 22-23 0.588897008303725 1106 24-25 0.5277526960185028 1106 26-27 0.4052803471357507 1106 28-29 0.39210401484331214 1106 30-31 0.5201001942725227 1106 32-33 0.48244268297934667 1106 34-35 0.453585072071526 1106 36-37 0.5098063159620665 1106 38-39 0.5316331470669695 1106 40-41 0.5088091024345189 1106 42-43 0.5277337110504945 1106 44-45 0.5707230513932231 1106 46-47 0.5984836533544637 1106 48-49 0.6045611240370263 1106 50-51 0.40699986435270574 1106 52-53 0.5044751897326591 1106 54-55 0.4962812147161344 1106 56-57 0.46580897492032847 1106 58-59 0.47878449883088336 1106 60-61 0.6542927593141386 1106 62-63 0.789383609436463 1106 64-65 0.8134431066517323 1106 66-67 0.9049567315272427 1106 68-69 0.8143883143262372 1106 70-71 0.5696595999724892 1106 72-73 0.3748634769734913 1106 74-75 0.7591240364770258 1106 76-77 0.6320578345181609 1106 78-79 0.47861099257487183 1106 80-81 0.7473861626573886 1106 82-83 0.6289692715959028 1106 84-85 0.6967100589801554 1106 86-87 0.7398622822141796 1106 88-89 0.6546130577717832 1106 90-91 0.5549758713901092 1106 92-93 0.5167487755091251 1106 94-95 0.5763292380245879 1106 96-97 0.6128666856745433 1106 98-99 0.6044438243733339 1106 100-101 0.5158001532330267 1107 1 0.26713142854245575 1107 2 0.2135518215271226 1107 3 0.331884917983281 1107 4 0.28471885546658626 1107 5 0.29502375866334773 1107 6 0.31602467345011576 1107 7 0.391365570357749 1107 8 0.358451902935883 1107 9 0.5722046301569463 1107 10-11 0.4804341089849302 1107 12-13 0.40577787650855157 1107 14-15 0.07290805008589984 1107 16-17 0.35080079112746176 1107 18-19 0.30841235313378235 1107 20-21 0.3275712366749346 1107 22-23 0.29135529952653627 1107 24-25 0.3039034618537002 1107 26-27 0.2510722022459504 1107 28-29 0.2323269571741733 1107 30-31 0.27909877068726274 1107 32-33 0.0492133742595513 1107 34-35 0.17394296078824567 1107 36-37 0.36218702746564446 1107 38-39 0.1610854641868471 1107 40-41 0.13724508910092226 1107 42-43 0.171961274097562 1107 44-45 0.241797429852177 1107 46-47 0.25547651271682525 1107 48-49 0.3721337304987955 1107 50-51 0.1472636870146573 1107 52-53 0.2598234416339551 1107 54-55 0.42238485461410136 1107 56-57 0.23823663686476237 1107 58-59 0.08782813651965427 1107 60-61 0.40027363433904384 1107 62-63 0.46827222188989026 1107 64-65 0.39756935667625015 1107 66-67 0.5322700575860821 1107 68-69 0.5418646455436331 1107 70-71 0.44637391562439177 1107 72-73 0.42272753129065066 1107 74-75 0.34778531919032574 1107 76-77 0.28132317573988885 1107 78-79 0.2603859009296343 1107 80-81 0.3160109214789202 1107 82-83 0.2967918055363903 1107 84-85 0.09307325801058752 1107 86-87 0.18628685335782436 1107 88-89 0.0019208918265469777 1107 90-91 0.0899677830720762 1107 92-93 0.10303569555814107 1107 94-95 0.19039774974309864 1107 96-97 0.16148279355732242 1107 98-99 0.3315650335226792 1107 100-101 0.28981141184138437 1108 1 0.22059851547653508 1108 2 0.303719864911308 1108 3 0.27643453524826 1108 4 0.23836819007630083 1108 5 0.13769796836709247 1108 6 0.23501282481791463 1108 7 0.3082734457223921 1108 8 0.18285157342970138 1108 9 0.32620007143177077 1108 10-11 0.399260800535437 1108 12-13 0.12640604833338642 1108 14-15 0.4087505052407536 1108 16-17 0.41824360738008437 1108 18-19 0.4176339216022882 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2316 80-81 -2.2231149950549067 2316 82-83 -2.3421728861647395 2316 84-85 -2.0885413825677652 2316 86-87 -1.8278262194487098 2316 88-89 -2.228054441359003 2316 90-91 -2.382592629847455 2316 92-93 -3.0058821374655373 2316 94-95 -2.830779221791733 2316 96-97 -3.362710109040325 2316 98-99 -4.298672479116817 2316 100-101 -5.063475402355518 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5280.0 3 2156.0 4 2244.0 5 2449.0 6 2768.0 7 3289.0 8 4140.0 9 5447.0 10 6584.0 11 8239.0 12 10198.0 13 12223.0 14 13501.0 15 15910.0 16 19233.0 17 23909.0 18 19808.0 19 16024.0 20 16257.0 21 17670.0 22 19693.0 23 22373.0 24 25260.0 25 29069.0 26 34105.0 27 40128.0 28 47458.0 29 56806.0 30 68606.0 31 83973.0 32 104699.0 33 132140.0 34 172913.0 35 233710.0 36 322556.0 37 413183.0 38 311091.0 39 42494.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81430775839965 18.264704006326113 14.169923850952824 22.751064384321413 2 15.509897184181401 19.982976674241975 40.01389745452702 24.4932286870496 3 20.1442751035681 24.202936446550748 29.392556783451745 26.260231666429405 4 12.644295801005919 16.18453302782291 37.64135982554814 33.52981134562303 5 13.716013846058297 37.276156024611275 34.04431059752425 14.963519531806178 6 31.763286044856727 35.65719037823457 17.632311106678813 14.947212470229895 7 28.686994636061936 32.455774649553085 20.5612134774699 18.29601723691508 8 26.47013344531936 33.642153950929504 19.725284985563775 20.162427618187365 9 26.185934121836453 17.1451471904876 20.70346714056287 35.965451547113084 10-11 25.22537539678767 26.124996698937853 27.119604083810007 21.53002382046447 12-13 26.521560488193884 23.617915933900534 27.472577382744216 22.38794619516137 14-15 23.072002717442395 25.136426635075836 26.495359075664 25.29621157181777 16-17 22.15393604679165 28.372592544397396 26.90315000350667 22.57032140530428 18-19 21.953778539348107 27.34549367374175 28.933025607098152 21.767702179811995 20-21 23.096258463010397 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22.94332598471891 46-47 22.95969364492301 26.653804022209183 27.99848959665346 22.38801273621435 48-49 22.831145310033087 26.66764797561736 27.875671675706815 22.625535038642735 50-51 22.126282149838563 26.98542095739486 28.27173128876439 22.616565604002183 52-53 22.738293104795535 27.26248830524833 27.378221468501785 22.62099712145435 54-55 22.260483793813282 26.68215573930966 27.81492353352759 23.24243693334947 56-57 22.758336244178135 26.804340122394866 27.476333459059305 22.960990174367698 58-59 23.25152763964917 26.586790608244833 27.44739667141085 22.714285080695152 60-61 22.732609132265992 26.803880080007485 28.225658107178013 22.23785268054851 62-63 22.42686764732591 26.93341037366673 28.062448286042645 22.577273692964706 64-65 22.184830541065253 27.33775148838719 27.643274491944197 22.83414347860336 66-67 22.43294021728225 27.042019096420976 27.235729169196908 23.28931151709986 68-69 22.259976384106235 26.873125100221774 27.785563121176914 23.081335394495074 70-71 22.757421137048077 26.72663681681812 27.53362228144487 22.98231976468893 72-73 22.623108504795244 26.525562881007893 28.01094185171683 22.840386762480037 74-75 22.138551449005178 27.60557399870927 27.417774224111252 22.838100328174296 76-77 22.466980825177295 26.894034452660335 27.40388718346502 23.23509753869735 78-79 22.566045661610318 26.971026689997057 27.472484980233897 22.99044266815873 80-81 22.848337311690283 27.06720626347107 27.191333536513458 22.893122888325188 82-83 22.66702359894222 26.923145184668083 27.681091012659405 22.728740203730293 84-85 22.478530857331506 27.185742170049426 27.373391886916753 22.962335085702314 86-87 22.40059684455617 27.260351258736637 27.39541015214509 22.943641744562104 88-89 22.34446811629653 27.21275136908783 26.916796890017142 23.525983624598503 90-91 22.491610674450225 26.831433499331137 27.251068788666743 23.4258870375519 92-93 22.81658473131803 26.92466038884102 27.448760745895086 22.809994133945864 94-95 22.428865678022305 26.700410006074478 27.60004933928446 23.270674976618754 96-97 21.991096496304852 26.772969949618908 28.051760803461367 23.184172750614874 98-99 22.123791525912203 27.12257872037606 27.174344810333274 23.57928494337846 100-101 21.991846895776362 26.756848142622264 27.354763585337093 23.89654137626428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 166.0 1 137.5 2 176.0 3 254.5 4 232.0 5 217.5 6 394.0 7 793.5 8 1304.0 9 1660.5 10 1616.0 11 1560.0 12 1696.5 13 1845.0 14 2089.5 15 2279.0 16 2386.5 17 2516.0 18 2715.0 19 3058.5 20 3453.0 21 3960.0 22 4679.5 23 5540.5 24 6682.0 25 8329.5 26 10675.5 27 13927.0 28 16968.5 29 20320.0 30 25243.5 31 30793.5 32 36412.5 33 41467.0 34 46163.0 35 50359.5 36 55700.0 37 60974.5 38 63542.0 39 65229.5 40 67058.0 41 69979.5 42 73324.0 43 76855.0 44 80631.5 45 84478.0 46 86483.5 47 94834.0 48 113566.0 49 112645.5 50 94819.0 51 85012.5 52 80398.0 53 78615.0 54 76557.5 55 71978.5 56 66059.0 57 60052.5 58 54973.0 59 48761.0 60 41322.0 61 34396.0 62 28330.0 63 22267.5 64 16554.0 65 12528.0 66 9367.0 67 7335.0 68 5497.0 69 3808.0 70 2834.5 71 2146.5 72 1678.0 73 1422.5 74 1156.0 75 821.5 76 560.5 77 355.5 78 210.0 79 135.0 80 99.5 81 72.5 82 50.5 83 29.5 84 25.0 85 19.0 86 10.5 87 9.5 88 9.0 89 9.5 90 9.5 91 6.5 92 2.5 93 2.5 94 2.5 95 1.0 96 1.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011488412542136497 2 0.011108281244786394 3 0.013515779461337057 4 0.01761275011055485 5 0.030410503788008376 6 0.047938780276929875 7 0.005321838162901466 8 0.02707379573349079 9 0.03547892108600978 10-11 0.039723720573085944 12-13 0.030220438139333325 14-15 0.028362018463399478 16-17 0.028932215409424637 18-19 0.023821561300606562 20-21 0.05389417060208152 22-23 0.012417622380103422 24-25 0.0032944712437009073 26-27 0.0014571699731754015 28-29 2.1118405408339152E-5 30-31 0.0024074982165506634 32-33 0.0016894724326671322 34-35 0.0 36-37 3.3789448653342644E-4 38-39 0.0 40-41 0.0012882227299086883 42-43 0.0035901289194176554 44-45 0.0015416435948087583 46-47 9.925650541919402E-4 48-49 7.180257838835311E-4 50-51 0.0040124970275844385 52-53 0.003716839351867691 54-55 0.005385193379126484 56-57 8.447362163335661E-4 58-59 0.0060609823521933365 60-61 0.003146642405842534 62-63 5.068417298001397E-4 64-65 9.925650541919402E-4 66-67 0.01712702678616305 68-69 0.041244245762486365 70-71 0.050832001817872334 72-73 0.015289725515637545 74-75 0.03043162219341672 76-77 0.027242742976757505 78-79 0.055097919710356845 80-81 0.07965862520025528 82-83 0.04819220114182994 84-85 0.018013999813313294 86-87 0.020590445273130673 88-89 0.021794194381406003 90-91 0.03822431378909386 92-93 0.02510978403051525 94-95 0.013558016272153736 96-97 0.018795380813421848 98-99 0.0502195680610305 100-101 0.06897271206363567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2367603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.187895089229784 #Duplication Level Percentage of deduplicated Percentage of total 1 80.21096925432704 48.277294024848715 2 11.975563335137032 14.415678992993088 3 3.480258659655329 6.284083292721553 4 1.4491133642291998 3.4887633255451163 5 0.7495895866048181 2.2558109699274955 6 0.4488290456860054 1.6208445308849049 7 0.2899891710474882 1.221768646281325 8 0.23087146882297954 1.1116534195691103 9 0.1596489094327947 0.8648038630845893 >10 0.896582700465514 9.928856175317518 >50 0.07185478860383791 2.99095491470412 >100 0.03363762353146414 3.7012943129315348 >500 0.0014757713997073762 0.5996860165437738 >1k 0.0012649469140341007 1.666457142459902 >5k 2.810993142298001E-4 1.0927414471294086 >10k+ 7.027482855745002E-5 0.47930892505788497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11332 0.4786275401745985 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7548 0.31880344804428784 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7292 0.3079908244752182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5917 0.24991520960228553 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5078 0.21447852532709244 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4742 0.20028695689268852 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3517 0.14854686364225758 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3364 0.1420846315873058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3285 0.13874792353278823 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2794 0.11800964942179919 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2549 0.10766163077171298 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2390 0.10094597785186114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.2236810816678304E-5 0.0 0.0 0.0 0.0 9 4.2236810816678304E-5 0.0 0.0 0.0 0.0 10-11 4.2236810816678304E-5 0.0 0.0 0.0 0.0 12-13 4.2236810816678304E-5 0.0 0.0 0.0 0.0 14-15 4.2236810816678304E-5 0.0 0.0 0.0 0.0 16-17 4.2236810816678304E-5 0.0 0.0 2.1118405408339152E-5 0.0 18-19 4.2236810816678304E-5 0.0 0.0 4.2236810816678304E-5 0.0 20-21 4.2236810816678304E-5 0.0 0.0 4.2236810816678304E-5 0.0 22-23 4.2236810816678304E-5 0.0 0.0 4.2236810816678304E-5 0.0 24-25 4.2236810816678304E-5 0.0 0.0 1.6894724326671322E-4 0.0 26-27 4.2236810816678304E-5 0.0 0.0 3.8013129735010474E-4 0.0 28-29 8.447362163335661E-5 0.0 0.0 7.602625947002095E-4 0.0 30-31 8.447362163335661E-5 0.0 0.0 0.0014994067839920798 0.0 32-33 8.447362163335661E-5 0.0 0.0 0.002365261405733985 0.0 34-35 1.0559202704169576E-4 0.0 0.0 0.003590128919417656 0.0 36-37 1.267104324500349E-4 0.0 0.0 0.004920588460143023 0.0 38-39 1.6894724326671322E-4 2.1118405408339152E-5 0.0 0.006082100757601676 0.0 40-41 1.6894724326671322E-4 4.2236810816678304E-5 0.0 0.00770821797404379 0.0 42-43 2.1118405408339152E-4 6.335521622501746E-5 0.0 0.010538084298761238 0.0 44-45 2.534208649000698E-4 8.447362163335661E-5 0.0 0.015543146380537616 0.0 46-47 2.534208649000698E-4 8.447362163335661E-5 0.0 0.027052677328082454 0.0 48-49 2.534208649000698E-4 8.447362163335661E-5 0.0 0.04975496314204704 0.0 50-51 2.534208649000698E-4 8.447362163335661E-5 0.0 0.06827580468516048 0.0 52-53 2.534208649000698E-4 8.447362163335661E-5 0.0 0.08728236955266572 0.0 54-55 2.9565767571674813E-4 8.447362163335661E-5 0.0 0.11306794255624782 0.0 56-57 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.13414411115377028 0.0 58-59 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.15872593504907706 0.0 60-61 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.19910432618982152 0.0 62-63 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.22932476432915483 0.0 64-65 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.26459250136108126 0.0 66-67 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.3336074502355336 0.0 68-69 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.413709561949364 0.0 70-71 3.3789448653342644E-4 8.447362163335661E-5 0.0 0.4761355683364145 0.0 72-73 3.590128919417656E-4 8.447362163335661E-5 0.0 0.5492263694546763 0.0 74-75 3.8013129735010474E-4 8.447362163335661E-5 0.0 0.6128350065445938 0.0 76-77 3.8013129735010474E-4 1.267104324500349E-4 0.0 0.6697913459308846 0.0 78-79 3.8013129735010474E-4 1.267104324500349E-4 0.0 0.7434945808059882 0.0 80-81 4.6460491898346135E-4 1.4782883785837407E-4 0.0 0.861673177471054 0.0 82-83 4.6460491898346135E-4 1.6894724326671322E-4 0.0 0.9776343415682442 0.0 84-85 4.6460491898346135E-4 1.6894724326671322E-4 0.0 1.1110815453435394 0.0 86-87 4.857233243918005E-4 1.6894724326671322E-4 0.0 1.3508599203498222 0.0 88-89 5.068417298001397E-4 1.6894724326671322E-4 0.0 1.6474256874991289 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 2240 0.0 26.508305 1 TACCTGG 2195 0.0 25.5374 2 GTACATG 10540 0.0 24.247204 1 TACATGG 10685 0.0 23.11841 2 GTGTAGC 1500 0.0 22.4847 1 ACATGGG 10465 0.0 22.288958 3 GAGTACT 7550 0.0 21.553194 12-13 CATGGGG 4990 0.0 20.46977 4 GTACTTT 7900 0.0 20.358816 14-15 AGAGTAC 13530 0.0 20.173918 10-11 AGTACTT 7885 0.0 19.552893 12-13 TAGCCCT 1625 0.0 17.249388 4 ACTTTTT 9745 0.0 17.188198 16-17 TACTTTT 9075 0.0 16.780401 14-15 GTATCAA 23950 0.0 16.700388 1 GTAGCCC 1400 0.0 16.627066 3 GTACACG 345 2.0962034E-8 16.522743 1 TACACTG 1935 0.0 16.20459 5 GAGTACA 8695 0.0 16.171194 1 AGTACAT 8130 0.0 15.951491 2 >>END_MODULE